AT1G23230

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009426
Physical Location & Seq
Forward (+)
8244418 .. 8251937
7520 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G23230.3

Sequence Viewer

Length: 4848 bp
ATGGATCAATCGCAGCGAACAGTCGCTGCAACGCCATCGTCTTCTCGATCGTACCAATTCCACCCAGCTCGTGCTGCAATTATCGATCTTTTCAATCTCTACTTAGGGAGAGGAAGCCGTCAAAAGCCTGATGAGTCCCTCCGAGATCCCCCGAACAAATCACAGAAGCGTGTTCATGCTCCTAATAGAGACTTACCACCAAGAAACGAGCAATTCCTTTTGGATTTTGAGCTGCTTCAGAGCCAATTCAATGATCCAGAGCAATTGAGGACGATTACTGAGTCAGTTTTGATATCTCTGGTGGTGCAATGTAGCAATCATGCGCCTCGTGCTGAGTTTCTACTCTTTGCTTTGCGTACATTGTGTAGGATTAGTTATATCAATTGGGATACTTTTTTACCATCCCTTCTCTCTTCCGTTTCCGCTGCTGAGGCGTCGTTAAGCCAAGGTGTTCAAGCAGCAGCGGCAACGGCTGGTTCTTCTGCTACTTCTTCGCAGTCTGTAGTTCCTGTGTCTGTGAATCCAACGTCATTGCTGCCTTCAGCTCATGGTATTGGTTCTCCATCAGCTTCAGAGGTGAAATCTGTGGAGAATGGGCAGCAGATTGCAAGAGCTGGCCAGATTGTAAGAGAGAATGCTATGAGAAATAGTCAACGGATTAGAGCTGCTGCTGTAAACAGTCTGCGTCAGCTCAGTTGCAAGATTATCTTGATTGGCGTTGAATCTAGTCTCAAACCCGTTACTCATGCCGAGATTTTCCAGTACATGATGAATTGGCTTGTTAACTGGGACAGGAGAGATCTGGGAACTGAGGACTCTGTAGGAAAGTCGTGGAGATCTGAGAAGACTTTAGCTGAATGGTTGCGGAGTTGTTTGGATGTGATCTGGCTGTTGGTAGAAGAGGGTGAATCTCGAATTCCGTTTTATGAGTTATTGCGCAGTGGTCTACAGTTTATAGAGAACATACCTGATGATGAAGCCTTGTTCACTCTTATCATGGAGATACACCGGAGGCGAGATGCGATGGCCATGCATATGCTCATGTTGGACCAACATCTTCACTGTCCATCATTTGGTACCCATCGTATAGTCTCCCAAATAACTGCCAATGTTCCACCCGAAGCTGTGCCACACTTACGACATTCACCAATTACATATCCAAGTGTGCTTGGGGAACCTTTATATGGAGAGGATCTGGCGATGTCTATTCCAAAAGGAAGCCTGGATTGGGAGAGAGCAGTGCGTTGCATTAGACATGCTATTCGCACTACACCGTCACCGGACTGGTGGAAGCGTGTTCTTGTTGTGGCCCCTTGTTATAGACCATCTACACAAGCAGGGCCTATCCCTGGTGCTGTATTCACTTCTGACATGATTTGTGAGGCAATCATTGACAGAATTGTTGAGCTTCTTAAGCTGACCAACTCAGGCAATGATTGCTTTGGTATTGATTTGGTATCTGTTACTTTTCCCCCTCTCTATGCAGATGCAAATTGCTGGCAAGAGTGGCTGGTTTTCTCGGATATATTCTTCTTTCTAATCAAAAGTGGGTGTACTGATTTTGTTGATTTCATTGACAAGTTGGTTTTACGCCTTAACGGTGTTGATAACCATATTCTTCGGACGAATCATGTGACTTGGCTGCTTGCACAAATAATACGCGTGGAGCTTGTGATGACTGCTTTGAACTCAGATGCTAAAAAGGTGGAGACTACTAGGAAGATCTTATCATTTCACAGGGAAGACAGAAATTCTGATCCCAACAATCCTCAGAGTGTGTTGCTTGACTTCGTAAGCAGTTGTCAGAATCTACGGATTTGGTCACTAAGCACAACAACTAGGGCATACCTAAACAATGAACAGCTGTTGAAGGGAAAACAGATCGATGAGTGGTGGAGAAGCAAAGGAGAGCGGATGATGGATTATATGAATATGGATGATAGGTCGATTGGCATGTTTTGGGTTGTTTCCTACACCATGGCACAACCAGCATGTGAAACAGTTATAAATTGGCTATCTTCAGCTGGCATGGCTGAGTTACCGGGATTACAGCCAAACGACCGAGTAATGATGACGCAGGAAGTTACCCCGTTACCTATGTCATTGCTATCTGGCTTTTCAATGAATTTGTGCTTGAAATTGGCCCTTCAGATGGAAGAAGCTTTGTTTGTTAGCCAGGTTGTTCCTAGCATTGCAATGGTTGAGACATACACAAGATTGCTTCTGATTTCCCCTCATTCGATGTTCCGTTCACATTTCAGTCAATTGGCCCAGCGAAATGCTTCTCTGCTAAGCAAGCCTGGAGTGACGTTACTTGTTCTTGAGATTCTGAACTATCGGTTGCTGCCACTCTACAGATACCAAGGAAAGAGTAAAACTTTAATGTATGATGTTACTAAAATAATATCTGCATTGAAAGGAAAACGTGGTGACCACCGTATATTCAGACTAGCTGAAAACTTATGCATGAATCTCATTTTATCACTCAGAGACTTTTTCTCTGTGAAACGGGAGGGAAAAGGGCCAACTGAATTCACTGAGACGTTAAATCGCATAACCATCATGACTCTTGCTATCACTATCAAAACACGTGGTATCGCAGACCCTGATCACATGGTTTATCTGCAAACCATGTTGGAACAAATACTCGCGACGAGTCAGCATACATGGTCAGAGAAGACGATGCGGCATTTCCCATCCCTTCTCCGTGAAACTTTGAAAGGACGGGTAGACAAGAGGGGCCTATCAATTCAGGCATGGCAACAGGCTGAAACAACTGTGATAAACCAGTGCACTCAACTTCTCTCACCATCTGCTGAACCCGCTTACGTTTCAACATACCTCAGTCACAGTTTTCCTCAACACCGTCAGTATCTATGTGCTGGTGCTTGTCTGCTGATGCAAGGCCACGCTGAAAACATCAACAGCACAAATCTGGCACGAGTTCTGAGAGAAGTTTCTCCTGAAGAAGTCACTGCTAACATATACACTTTGGTCGATGTTTTGCTTCACCACGTTCATGTAGACCTTCAGCAAGGACAATCTCTAGAGGCAGTTCTAGACAAGGCAGGCGCAAATCTTGCATTTTTCTTCTGGACACATGAAATGCTTCCTCTCGACATTTTTCTTTTGGCGCTCATTGACCGTGATGATGATCCTCACGCCTTGATAATTGCAATGAGCCTACTTAAAACGCCAGACCTTCTGCTGAGGATAAAAAACTACTGCCAAAACCGTGGGTCTCCTGAGCACTGGCTTGTCACACAGGTGTTCAAACGCAATGAACTCCAGAAGGCCCTGGGTAACCATCTTTCTTGGAAGGACAGGTATCCAACGTTCTTTGATGATATTGCAGCGCGTTTGCTTCCAGTTATCCCTCTAGTGCTTTACAGGCTCATCGAGAACAATGCGATGGAGCAAGCTGATAATCTTTTGCTTGCACACTCGCATTTTCTAGCGTATCATCCTCTCAGATTCACGTTTGTCCGTGATATACTAGCCTATTTCTATGGCCATCTTCCTGGGAAACTTGTTCTGCGCATGCTCAAAGTACTTGATCTCAGCAAGATTCCATTCTCTGAATCATTCCCGCAGTACATTAGCCCTACCGGTGCACCTGTATGCCCACCTCTAGATTACTTTGCCTCTCTTCTGCTTAATCTCGTAAACAATGTTATACCTCCACTTAGCAGCAGCAGCAACTGCAGCTCGAGGTCTGGCTCAATGGCAGACATCTTAAACAGCTCAGCAAGACCTCCTCATGGAAAAACTCCAGGAACATCTCAGCCTGGACCAGCAAACGCCTCTGAGGGCCAGAAAGCATTCTATCAGATCCAGGACCCAGGAACTTACACGCAACTGGTTCTTGAGACAGCCGTGATCGAGATCCTCTCGCTTCCTGTCTCTGCTGCTCAGATTGTCTCTTCGCTTGTCCAGATAATTGTCAACATACAATCGACTCTGATTCAATCTGGAAACGGGTTCCACGGCGCTGCCAACGGGGTGGGACAAGGGTCGGTACTGCCAACGTCTCCCTCTGGAGGGAGCACAGACTCCATGAGCGCTAGCCGGTCCACTTGTCTGATTCCTGGTATCAACACAGCGAGCTTTGTCTCTAGAAGCGGTTATACATGTCAGCAACTGTCGTGTCTGTTGATTCAAGCTTGTGGACTTCTGTTGGCTCAGCTCCCTCCAGATTTTCACGTACAGCTTTACTTGGAGGCAGCACGTGTGACAAGGGAAACATGGTGGCTTAAGGACGGGAAGAGATCACAAGGTGAACTAGACTCAGCTGTTGGATACGCTTTGATGGATCCTACATGGGCTGCTCAGGATAACACCTCGACCGCCATAGGAAATATTGTCGCCTTGCTTCATGCTTTCTTCAGCAATCTCCCACAAGAATGGCTTGATGGCACGAATGCTATCATCACGAACCTCCGGCCTGTGACATCTGTGGCAATGCTCAGAGTTGTATTCCGTATCATGGGGCCACTGCTTCCGAGGCTTGCCAGTACACATACTCTCTTTAACAAGACGCTAATGCTTCTCTTAAGCGCACTGGTCGATGTTTTTGGAAAGACCGCACAGACAACAGCCCCTGTTGAAGCATCCCAGATTGCAGATCTTATTGATTTCCTACACCATATTATTCACTACGAGGGACAAGGAGGAGCTGTTCAAACTAGCAGCAAGCCGAGACCGGACATCTTGGCGTTAATCGGAAGAGCAGCAGAGACTCTCCGACCAGATGTACAACACCTTCTCGCTCACCTCAAAACCAACCCGAATTCTTCCATATACGCAGCTGCTCATCAGCAGAATACTGCAAAGACTAACACCTCTTAA

Protein Analysis

1615

Amino Acids

180.09

Weight (kDa)

6.96

Isoelectric Point (pI)

43.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med23 PF11573 496 - 1205 1.2e-32 Mediator complex subunit 23
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2006
Acc16I TGCGCA 2 cut(s) 938, 3539
Acc65I GGTACC 1 cut(s) 1076
AccB1I GGYRCC 1 cut(s) 1076
AccB7I CCANNNNNTGG 1 cut(s) 1073
AccBSI CCGCTC 1 cut(s) 1912
AccI GTMKAC 3 cut(s) 946, 2730, 3024
AccII CGCG 3 cut(s) 1662, 2651, 3358
AclI AACGTT 1 cut(s) 3335
AcoI YGGCCR 3 cut(s) 616, 1026, 3511
AcsI RAATTY 5 cut(s) 915, 1750, 2125, 2531, 4789
AcuI CTGAAG 8 cut(s) 221, 525, 555, 2004, 2132, 2985, 3014, 4369
AcvI CACGTG 2 cut(s) 2591, 4229
AcyI GRCGYC 1 cut(s) 434
AdeI CACNNNGTG 1 cut(s) 4277
AfeI AGCGCT 1 cut(s) 4063
AfiI CCNNNNNNNGG 9 cut(s) 1073, 1184, 1228, 1349, 2152, 3761, 4040, 4041, 4486
AflII CTTAAG 3 cut(s) 1412, 4253, 4552
AflIII ACRYGT 4 cut(s) 1660, 2588, 4130, 4228
AgeI ACCGGT 1 cut(s) 3608
AjuI GAANNNNNNNTTGG 2 cut(s) 1210, 1242
AleI CACNNNNGTG 1 cut(s) 3266
AloI GAACNNNNNNTCC 2 cut(s) 4662, 4694
Alw21I GWGCWC 4 cut(s) 2795, 3252, 3616, 4049
Alw44I GTGCAC 2 cut(s) 2791, 3612
AlwNI CAGNNNCTG 5 cut(s) 26, 2606, 3702, 4141, 4601
Ama87I CYCGRG 1 cut(s) 3709
Aor51HI AGCGCT 1 cut(s) 4063
ApaLI GTGCAC 2 cut(s) 2791, 3612
ApoI RAATTY 5 cut(s) 915, 1750, 2125, 2531, 4789
ArsI GACNNNNNNTTYG 2 cut(s) 1245, 1277
AsiGI ACCGGT 1 cut(s) 3608
Asp700I GAANNNNTTC 3 cut(s) 2281, 3108, 3821
Asp718I GGTACC 1 cut(s) 1076
AspLEI GCGC 9 cut(s) 325, 939, 3074, 3136, 3358, 3540, 3992, 4064, 4559
AsuC2I CCSGG 1 cut(s) 2043
AsuHPI GGTGA 9 cut(s) 589, 917, 1137, 1269, 2441, 2799, 3002, 4289, 4763
AsuNHI GCTAGC 1 cut(s) 4064
AvaI CYCGRG 1 cut(s) 3709
AvaII GGWCC 4 cut(s) 1048, 3791, 3838, 4071
BaeGI GKGCMC 2 cut(s) 2795, 3616
BalI TGGCCA 3 cut(s) 618, 1028, 3513
BamHI GGATCC 1 cut(s) 4312
BanI GGYRCC 1 cut(s) 1076
BarI GAAGNNNNNNTAC 4 cut(s) 4111, 4143, 4746, 4778
BauI CACGAG 3 cut(s) 69, 327, 2940
BbrPI CACGTG 2 cut(s) 2591, 4229
BbsI GAAGAC 4 cut(s) 33, 851, 1749, 2684
Bbv12I GWGCWC 4 cut(s) 2795, 3252, 3616, 4049
BbvCI CCTCAGC 2 cut(s) 429, 3209
BceAI ACGGC 4 cut(s) 102, 486, 3860, 4003
BcgI CGANNNNNNTGC 6 cut(s) 18, 52, 1012, 1046, 3389, 3423
BciVI GTATCC 3 cut(s) 382, 3339, 4292
BclI TGATCA 1 cut(s) 2608
BcnI CCSGG 1 cut(s) 2043
BfmI CTRYAG 5 cut(s) 501, 819, 947, 2353, 3703
BfoI RGCGCY 3 cut(s) 3137, 3993, 4065
BfrI CTTAAG 3 cut(s) 1412, 4253, 4552
BfuI GTATCC 3 cut(s) 382, 3339, 4292
BglII AGATCT 4 cut(s) 799, 836, 1722, 4624
BlpI GCTNAGC 3 cut(s) 2291, 3745, 4182
BmcAI AGTACT 1 cut(s) 3552
Bme18I GGWCC 4 cut(s) 1048, 3791, 3838, 4071
BmeT110I CYCGRG 1 cut(s) 3709
BmiI GGNNCC 8 cut(s) 1078, 1176, 1311, 2741, 3840, 3983, 4314, 4491
BmrI ACTGGG 1 cut(s) 796
BmsI GCATC 6 cut(s) 1009, 1477, 1684, 2673, 2889, 4619
BmtI GCTAGC 1 cut(s) 4068
BmuI ACTGGG 1 cut(s) 796
BoxI GACNNNNGTC 1 cut(s) 4012
BpiI GAAGAC 4 cut(s) 33, 851, 1749, 2684
BplI GAGNNNNNCTC 2 cut(s) 3875, 3907
BpmI CTGGAG 5 cut(s) 2322, 3272, 3756, 4059, 4176
Bpu10I CCTNAGC 4 cut(s) 429, 3209, 3246, 4329
Bpu1102I GCTNAGC 3 cut(s) 2291, 3745, 4182
BpuEI CTTGAG 2 cut(s) 2342, 3887
BpuMI CCSGG 1 cut(s) 2043
Bsa29I ATCGAT 2 cut(s) 84, 1884
BsaAI YACGTR 3 cut(s) 2591, 4204, 4229
BsaBI GATNNNNATC 2 cut(s) 3153, 3884
BsaHI GRCGYC 1 cut(s) 434
BsaI GGTCTC 2 cut(s) 3246, 4693
BsaWI WCCGGW 4 cut(s) 1008, 1279, 3608, 4702
BsaXI ACNNNNNCTCC 2 cut(s) 4662, 4692
Bsc4I CCNNNNNNNGG 9 cut(s) 1073, 1184, 1228, 1349, 2152, 3761, 4040, 4041, 4486
Bse118I RCCGGY 2 cut(s) 3608, 4068
Bse1I ACTGG 9 cut(s) 760, 791, 1289, 2788, 3257, 3368, 3864, 4512, 4566
Bse3DI GCAATG 9 cut(s) 314, 530, 1438, 2102, 2190, 2202, 3183, 3286, 4467
Bse8I GATNNNNATC 2 cut(s) 3153, 3884
BseCI ATCGAT 2 cut(s) 84, 1884
BseGI GGATG 7 cut(s) 401, 883, 1920, 1942, 2696, 3463, 4610
BseJI GATNNNNATC 2 cut(s) 3153, 3884
BseLI CCNNNNNNNGG 9 cut(s) 1073, 1184, 1228, 1349, 2152, 3761, 4040, 4041, 4486
BseMI GCAATG 9 cut(s) 314, 530, 1438, 2102, 2190, 2202, 3183, 3286, 4467
BseNI ACTGG 9 cut(s) 760, 791, 1289, 2788, 3257, 3368, 3864, 4512, 4566
BseRI GAGGAG 2 cut(s) 3747, 4686
BseSI GKGCMC 2 cut(s) 2795, 3616
BseYI CCCAGC 2 cut(s) 64, 2271
Bsh1236I CGCG 3 cut(s) 1662, 2651, 3358
Bsh1285I CGRYCG 3 cut(s) 50, 2062, 4347
BshNI GGYRCC 1 cut(s) 1076
BshTI ACCGGT 1 cut(s) 3608
BshVI ATCGAT 2 cut(s) 84, 1884
BsiEI CGRYCG 3 cut(s) 50, 2062, 4347
BsiHKAI GWGCWC 4 cut(s) 2795, 3252, 3616, 4049
BsiHKCI CYCGRG 1 cut(s) 3709
BsiSI CCGG 7 cut(s) 1009, 1280, 2042, 3609, 4069, 4441, 4703
BslFI GGGAC 4 cut(s) 121, 803, 4020, 4677
BslI CCNNNNNNNGG 9 cut(s) 1073, 1184, 1228, 1349, 2152, 3761, 4040, 4041, 4486
BsmBI CGTCTC 2 cut(s) 2534, 4035
BsmFI GGGAC 4 cut(s) 121, 803, 4020, 4677
BsmI GAATGC 3 cut(s) 640, 3821, 4426
Bso31I GGTCTC 2 cut(s) 3246, 4693
BsoBI CYCGRG 1 cut(s) 3709
Bsp1286I GDGCHC 4 cut(s) 2795, 3252, 3616, 4049
Bsp1407I TGTACA 1 cut(s) 4753
Bsp1720I GCTNAGC 3 cut(s) 2291, 3745, 4182
Bsp19I CCATGG 1 cut(s) 1977
Bsp68I TCGCGA 1 cut(s) 2651
BspDI ATCGAT 2 cut(s) 84, 1884
BspFNI CGCG 3 cut(s) 1662, 2651, 3358
BspHI TCATGA 1 cut(s) 2562
BspLI GGNNCC 8 cut(s) 1078, 1176, 1311, 2741, 3840, 3983, 4314, 4491
BspMAI CTGCAG 1 cut(s) 3707
BspOI GCTAGC 1 cut(s) 4068
BspQI GCTCTTC 1 cut(s) 4720
BspT107I GGYRCC 1 cut(s) 1076
BspTI CTTAAG 3 cut(s) 1412, 4253, 4552
BspTNI GGTCTC 2 cut(s) 3246, 4693
BsrBI CCGCTC 1 cut(s) 1912
BsrDI GCAATG 9 cut(s) 314, 530, 1438, 2102, 2190, 2202, 3183, 3286, 4467
BsrFI RCCGGY 2 cut(s) 3608, 4068
BsrGI TGTACA 1 cut(s) 4753
BsrI ACTGG 9 cut(s) 760, 791, 1289, 2788, 3257, 3368, 3864, 4512, 4566
BssAI RCCGGY 2 cut(s) 3608, 4068
BssNI GRCGYC 1 cut(s) 434
BssSI CACGAG 3 cut(s) 69, 327, 2940
BssT1I CCWWGG 3 cut(s) 445, 1977, 2362
Bst2BI CACGAG 3 cut(s) 69, 327, 2940
Bst6I CTCTTC 6 cut(s) 418, 894, 3654, 3928, 4259, 4720
BstACI GRCGYC 1 cut(s) 434
BstAFI CTTAAG 3 cut(s) 1412, 4253, 4552
BstAPI GCANNNNNTGC 3 cut(s) 1437, 3080, 3702
BstAUI TGTACA 1 cut(s) 4753
BstBAI YACGTR 3 cut(s) 2591, 4204, 4229
BstDSI CCRYGG 3 cut(s) 1977, 3235, 3985
BstEII GGTNACC 2 cut(s) 2429, 3302
BstF5I GGATG 7 cut(s) 401, 883, 1920, 1942, 2696, 3463, 4610
BstFNI CGCG 3 cut(s) 1662, 2651, 3358
BstH2I RGCGCY 3 cut(s) 3137, 3993, 4065
BstHHI GCGC 9 cut(s) 325, 939, 3074, 3136, 3358, 3540, 3992, 4064, 4559
BstMCI CGRYCG 3 cut(s) 50, 2062, 4347
BstNSI RCATGY 5 cut(s) 1259, 1957, 1995, 3544, 4134
BstPAI GACNNNNGTC 1 cut(s) 4012
BstPI GGTNACC 2 cut(s) 2429, 3302
BstSFI CTRYAG 5 cut(s) 501, 819, 947, 2353, 3703
BstSLI GKGCMC 2 cut(s) 2795, 3616
BstUI CGCG 3 cut(s) 1662, 2651, 3358
BstV2I GAAGAC 4 cut(s) 33, 851, 1749, 2684
BstX2I RGATCY 9 cut(s) 145, 799, 836, 1192, 1722, 3831, 3885, 4312, 4624
BstXI CCANNNNNNTGG 4 cut(s) 3236, 3521, 4003, 4404
BstYI RGATCY 9 cut(s) 145, 799, 836, 1192, 1722, 3831, 3885, 4312, 4624
Bsu15I ATCGAT 2 cut(s) 84, 1884
BsuI GTATCC 3 cut(s) 382, 3339, 4292
BsuTUI ATCGAT 2 cut(s) 84, 1884
BtgI CCRYGG 3 cut(s) 1977, 3235, 3985
BtgZI GCGATG 3 cut(s) 1037, 1214, 3425
BtsCI GGATG 7 cut(s) 401, 883, 1920, 1942, 2696, 3463, 4610
BtsI GCAGTG 4 cut(s) 946, 1245, 2973, 4493
BtsIMutI CAGTG 9 cut(s) 946, 1060, 1245, 2535, 2795, 2973, 3250, 4493, 4559
BtuMI TCGCGA 1 cut(s) 2651
CaiI CAGNNNCTG 5 cut(s) 26, 2606, 3702, 4141, 4601
CciI TCATGA 1 cut(s) 2562
CfoI GCGC 9 cut(s) 325, 939, 3074, 3136, 3358, 3540, 3992, 4064, 4559
Cfr10I RCCGGY 2 cut(s) 3608, 4068
ClaI ATCGAT 2 cut(s) 84, 1884
CseI GACGC 4 cut(s) 423, 674, 2083, 4546
CspAI ACCGGT 1 cut(s) 3608
CspCI CAANNNNNGTGG 2 cut(s) 2572, 2607
DraIII CACNNNGTG 1 cut(s) 4277
EaeI YGGCCR 3 cut(s) 616, 1026, 3511
Eam1104I CTCTTC 6 cut(s) 418, 894, 3654, 3928, 4259, 4720
EarI CTCTTC 6 cut(s) 418, 894, 3654, 3928, 4259, 4720
Eco130I CCWWGG 3 cut(s) 445, 1977, 2362
Eco31I GGTCTC 2 cut(s) 3246, 4693
Eco32I GATATC 1 cut(s) 294
Eco47I GGWCC 4 cut(s) 1048, 3791, 3838, 4071
Eco47III AGCGCT 1 cut(s) 4063
Eco57I CTGAAG 8 cut(s) 221, 525, 555, 2004, 2132, 2985, 3014, 4369
Eco72I CACGTG 2 cut(s) 2591, 4229
Eco88I CYCGRG 1 cut(s) 3709
Eco91I GGTNACC 2 cut(s) 2429, 3302
EcoO109I RGGNCCY 4 cut(s) 1340, 2740, 3295, 3838
EcoO65I GGTNACC 2 cut(s) 2429, 3302
EcoRI GAATTC 3 cut(s) 915, 2531, 4789
EcoRV GATATC 1 cut(s) 294
EcoT14I CCWWGG 3 cut(s) 445, 1977, 2362
EcoT22I ATGCAT 2 cut(s) 1035, 2468
ErhI CCWWGG 3 cut(s) 445, 1977, 2362
Esp3I CGTCTC 2 cut(s) 2534, 4035
FalI AAGNNNNNCTT 4 cut(s) 692, 724, 4392, 4424
FaqI GGGAC 4 cut(s) 121, 803, 4020, 4677
FauI CCCGC 2 cut(s) 2830, 3597
FauNDI CATATG 1 cut(s) 1035
FbaI TGATCA 1 cut(s) 2608
FblI GTMKAC 3 cut(s) 946, 2730, 3024
FokI GGATG 7 cut(s) 388, 890, 1927, 1949, 2683, 3450, 4597
FspI TGCGCA 2 cut(s) 938, 3539
GlaI GCGC 9 cut(s) 324, 938, 3073, 3135, 3357, 3539, 3991, 4063, 4558
GsaI CCCAGC 2 cut(s) 68, 2275
GsuI CTGGAG 5 cut(s) 2322, 3272, 3756, 4059, 4176
HaeII RGCGCY 3 cut(s) 3137, 3993, 4065
HapII CCGG 7 cut(s) 1009, 1280, 2042, 3609, 4069, 4441, 4703
HgaI GACGC 4 cut(s) 423, 674, 2083, 4546
HhaI GCGC 9 cut(s) 325, 939, 3074, 3136, 3358, 3540, 3992, 4064, 4559
Hin1I GRCGYC 1 cut(s) 434
Hin6I GCGC 9 cut(s) 323, 937, 3072, 3134, 3356, 3538, 3990, 4062, 4557
HinP1I GCGC 9 cut(s) 323, 937, 3072, 3134, 3356, 3538, 3990, 4062, 4557
HincII GTYRAC 3 cut(s) 653, 784, 3946
HindII GTYRAC 3 cut(s) 653, 784, 3946
HindIII AAGCTT 2 cut(s) 2160, 4161
HpaI GTTAAC 1 cut(s) 784
HpaII CCGG 7 cut(s) 1009, 1280, 2042, 3609, 4069, 4441, 4703
HphI GGTGA 9 cut(s) 589, 917, 1137, 1269, 2441, 2799, 3002, 4289, 4763
Hpy99I CGWCG 2 cut(s) 439, 2656
Hsp92I GRCGYC 1 cut(s) 434
HspAI GCGC 9 cut(s) 323, 937, 3072, 3134, 3356, 3538, 3990, 4062, 4557
KpnI GGTACC 1 cut(s) 1080
Ksp22I TGATCA 1 cut(s) 2608
KspAI GTTAAC 1 cut(s) 784
LguI GCTCTTC 1 cut(s) 4720
LmnI GCTCC 6 cut(s) 184, 1666, 3415, 4044, 4191, 4673
LweI GCATC 6 cut(s) 1009, 1477, 1684, 2673, 2889, 4619
MbiI CCGCTC 1 cut(s) 1912
MfeI CAATTG 3 cut(s) 263, 382, 2264
MflI RGATCY 9 cut(s) 145, 799, 836, 1192, 1722, 3831, 3885, 4312, 4624
MhlI GDGCHC 4 cut(s) 2795, 3252, 3616, 4049
MlsI TGGCCA 3 cut(s) 618, 1028, 3513
MluI ACGCGT 1 cut(s) 1660
MluNI TGGCCA 3 cut(s) 618, 1028, 3513
MlyI GAGTC 9 cut(s) 143, 290, 809, 2560, 2665, 3952, 4046, 4280, 4732
MmeI TCCRAC 6 cut(s) 548, 1026, 2616, 3356, 4276, 4768
Mox20I TGGCCA 3 cut(s) 618, 1028, 3513
Mph1103I ATGCAT 2 cut(s) 1035, 2468
MroXI GAANNNNTTC 3 cut(s) 2281, 3108, 3821
MscI TGGCCA 3 cut(s) 618, 1028, 3513
MslI CAYNNNNRTG 6 cut(s) 1034, 2195, 3018, 3266, 3619, 4402
Msp20I TGGCCA 3 cut(s) 618, 1028, 3513
MspA1I CMGCKG 6 cut(s) 425, 464, 1864, 2024, 4292, 4808
MspCI CTTAAG 3 cut(s) 1412, 4253, 4552
MspI CCGG 7 cut(s) 1009, 1280, 2042, 3609, 4069, 4441, 4703
MunI CAATTG 3 cut(s) 263, 382, 2264
Mva1269I GAATGC 3 cut(s) 640, 3821, 4426
MvnI CGCG 3 cut(s) 1662, 2651, 3358
NciI CCSGG 1 cut(s) 2043
NcoI CCATGG 1 cut(s) 1977
NdeI CATATG 1 cut(s) 1035
NheI GCTAGC 1 cut(s) 4064
NlaIV GGNNCC 8 cut(s) 1078, 1176, 1311, 2741, 3840, 3983, 4314, 4491
NmeAIII GCCGAG 2 cut(s) 775, 4722
NruI TCGCGA 1 cut(s) 2651
NsbI TGCGCA 2 cut(s) 938, 3539
NsiI ATGCAT 2 cut(s) 1035, 2468
NspI RCATGY 5 cut(s) 1259, 1957, 1995, 3544, 4134
OliI CACNNNNGTG 1 cut(s) 3266
PaeI GCATGC 1 cut(s) 3544
PaeR7I CTCGAG 1 cut(s) 3709
PagI TCATGA 1 cut(s) 2562
PasI CCCWGGG 1 cut(s) 3298
PciI ACATGT 1 cut(s) 4130
PciSI GCTCTTC 1 cut(s) 4720
PctI GAATGC 3 cut(s) 640, 3821, 4426
PdmI GAANNNNTTC 3 cut(s) 2281, 3108, 3821
PflMI CCANNNNNTGG 1 cut(s) 1073
PfoI TCCNGGA 2 cut(s) 3772, 3834
PinAI ACCGGT 1 cut(s) 3608
Ple19I CGATCG 1 cut(s) 50
PleI GAGTC 9 cut(s) 142, 289, 809, 2560, 2664, 3952, 4046, 4280, 4732
PmaCI CACGTG 2 cut(s) 2591, 4229
PmlI CACGTG 2 cut(s) 2591, 4229
PpsI GAGTC 9 cut(s) 142, 289, 809, 2560, 2664, 3952, 4046, 4280, 4732
Ppu21I YACGTR 3 cut(s) 2591, 4204, 4229
PpuMI RGGWCCY 1 cut(s) 3838
PscI ACATGT 1 cut(s) 4130
PshAI GACNNNNGTC 1 cut(s) 4012
PsiI TTATAA 1 cut(s) 2006
Psp1406I AACGTT 1 cut(s) 3335
Psp5II RGGWCCY 1 cut(s) 3838
PspCI CACGTG 2 cut(s) 2591, 4229
PspEI GGTNACC 2 cut(s) 2429, 3302
PspFI CCCAGC 2 cut(s) 64, 2271
PspN4I GGNNCC 8 cut(s) 1078, 1176, 1311, 2741, 3840, 3983, 4314, 4491
PspPPI RGGWCCY 1 cut(s) 3838
PspXI VCTCGAGB 1 cut(s) 3709
PsrI GAACNNNNNNTAC 2 cut(s) 3320, 3352
PstI CTGCAG 1 cut(s) 3707
PstNI CAGNNNCTG 5 cut(s) 26, 2606, 3702, 4141, 4601
PsuI RGATCY 9 cut(s) 145, 799, 836, 1192, 1722, 3831, 3885, 4312, 4624
PvuI CGATCG 1 cut(s) 50
PvuII CAGCTG 4 cut(s) 1864, 2024, 4292, 4808
RruI TCGCGA 1 cut(s) 2651
RseI CAYNNNNRTG 6 cut(s) 1034, 2195, 3018, 3266, 3619, 4402
SapI GCTCTTC 1 cut(s) 4720
ScaI AGTACT 1 cut(s) 3552
SchI GAGTC 9 cut(s) 143, 290, 809, 2560, 2665, 3952, 4046, 4280, 4732
SduI GDGCHC 4 cut(s) 2795, 3252, 3616, 4049
SfaNI GCATC 6 cut(s) 1009, 1477, 1684, 2673, 2889, 4619
SfcI CTRYAG 5 cut(s) 501, 819, 947, 2353, 3703
Sfr274I CTCGAG 1 cut(s) 3709
SinI GGWCC 4 cut(s) 1048, 3791, 3838, 4071
SlaI CTCGAG 1 cut(s) 3709
SmiMI CAYNNNNRTG 6 cut(s) 1034, 2195, 3018, 3266, 3619, 4402
SmlI CTYRAG 6 cut(s) 1412, 2321, 3709, 3866, 4253, 4552
SmoI CTYRAG 6 cut(s) 1412, 2321, 3709, 3866, 4253, 4552
SphI GCATGC 1 cut(s) 3544
SspI AATATT 1 cut(s) 4360
StyI CCWWGG 3 cut(s) 445, 1977, 2362
TaqII GACCGA 1 cut(s) 2076
TatI WGTACW 6 cut(s) 762, 1553, 3550, 3594, 4514, 4753
TauI GCSGC 2 cut(s) 467, 2689
TscAI CASTG 9 cut(s) 946, 1067, 1245, 2542, 2795, 2980, 3257, 4500, 4566
TspGWI ACGGA 8 cut(s) 406, 670, 909, 1828, 2237, 2696, 3476, 4469
TspRI CASTG 9 cut(s) 946, 1067, 1245, 2542, 2795, 2980, 3257, 4500, 4566
Van91I CCANNNNNTGG 1 cut(s) 1073
Vha464I CTTAAG 3 cut(s) 1412, 4253, 4552
VneI GTGCAC 2 cut(s) 2791, 3612
VpaK11BI GGWCC 4 cut(s) 1048, 3791, 3838, 4071
XapI RAATTY 5 cut(s) 915, 1750, 2125, 2531, 4789
XbaI TCTAGA 4 cut(s) 3046, 3058, 3631, 4115
XceI RCATGY 5 cut(s) 1259, 1957, 1995, 3544, 4134
XhoI CTCGAG 1 cut(s) 3709
XmiI GTMKAC 3 cut(s) 946, 2730, 3024
XmnI GAANNNNTTC 3 cut(s) 2281, 3108, 3821
ZrmI AGTACT 1 cut(s) 3552
Zsp2I ATGCAT 2 cut(s) 1035, 2468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.