Rw4G034580

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0009426
Physical Location & Seq
Reverse (-)
61614707 .. 61624615
9909 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G034580.1

Sequence Viewer

Length: 4809 bp
ATGGATCAAAACCAGAGATCGTCGTCGGCCTCAGCTTCGCGAGCTTACCAGTTCCACCCTGCTCGCGCTGCAATCGTCAATCTCTTCGACCTCTACTTAGGAGGAAGCAGTAGTCGCCAGAAATCAGAGGATTCAAATCGCGAACCTCCGAACAAGTCACAGAAGCGTGTGCTTGCGCTCAATAGAGAACTTCCTCCTCGAAACGAGCAGTTCCTTCTCGATTTCGAGCAGCTCCAGAGCCAGTTTCCTGACCAAGATCAACTGCGTGTTGTGACAGAGTCTGTGCTTATATCTCTAGTTGTGCAGTGCAGTAATCATGCACCACGGGCAGAGTTTCTTCTCTTTGCTTTAAGGAGTTTGTGCACAATAGGTCACATTAACTGGGATAGTTTTTTGCCGTCCCTTCTTTCTTCTGTCTCCACAGCAGAAATGTCAATGGGTCAAGGGAGTAGTCAAGCAATGACGGGTATTTCATCACAGTCCAATATGCTTCCAAGTTCAAATGCAATTCCCAATTCATCTAACTTTCAATCTTCAAATCCTGCGTCTCCATTACCTACTGTTCATGGAATTGGTTCCCCCAGCCAGACAGCTATTGAACCTATGACTCTGTCACCTGCTAAATCATCTGATATGCCCAGTAACGGACAGCAGGCTGCAGCAAGGGCCAATTCATCTATAAGAGATAATGCAATCAGCAGTCTACGTCAGTTGTGTTGCAAAATTATTTTGACTGGACTGGAATTTAATTTAAAACCAGTAACTCATGCGGACATATTTTCTTATATGCTGAATTGGCTGGTTAATTGGGATCAAAAACAACTGGGGGCTGATGAATCTGATGGCATAAAGTCTTGGAGACCTGGGAAGGCACTAATTGAATGGCTGCATAGCTGTTTGGATGTCATTTGGCTTTTGGTTGACGAGGATAAATGTCGAGTTCCCTTCTATGAATTACTGCGTAGTGGCTTGCAATTCATGGAGAACATACCTGATGATGAAGCTTTGTTTACTCTAATCTTGGAGATCCATAGGAGGCGAGATATGATGGCTATGCACATGAAAATGCTAGATCAACACTTACATTGCCCTACATTTGGGACTCATCGAATTTTCTCGCAGACTACTCCTAGCATTTCAGGTGAAGCAGTGGCAAGCTTGCGGTATTCACCAATCACATATCCAAGTGTACTTGGGGAACCATTGCATGGAGAGGATCTTGCAATTTCTATTCCAAAAGGAAGTTTAGATTGGGAGAGAGCGTTGCGTTGCATAAGGCATGCTATTTGTACTACGCCATCACCTGACTGGTGGAAACGTGTGCTGCTTGTGGCTCCTTGTTATAGGGGTCCTTCTCAAGGACCTACCCCTGGTGCTGTTTTCACTTCTGAAATGATTTGTGAGGCAACAATTGATAGAATTGTTGAATTACTGAAGTTGACAAATTCAGATGTTAATTGCTGGCAAGACTGGCTTGTCTTTTCAGATATATTCTTTTTTCTTATAAAAAGCGGCTGCGTTGATTTCGTCTATTTTGTGGACAAGCTAGTTTCACGCCTTACAGAGAGTGATCCCCATATTCTTAAGACCAATCATGTCACTTGGCTTCTAGCTCAAATTATTCGGGTTGAGCTTGTGATAAATGCTCTAAATTCAGATGCTAGAAAGGTAGAGACAACTCGGAAGATCCTATCGCTTCATAAAGAAGATAGAAGCTCAGATCCAAATAGTCCTCAGAGTATCCTGCTTGACTTTATAAGCAGTTGCCAAAATTTACGTATTTGGTCATTGAATACAACAACTAGAGAATATCTGAATAATGAGCAGCTTCAGAAAGGAAAGCAAATAGATGAATGGTGGAGACATGCAAGCAAAGGGGATCGTATGATGGATTATATGAATATGGATGATAGGTCAATTGGGATGTTTTGGGTTGTATCTTACACTATGGCGCAACCAGCTTGTGAAACTGTGATCAATTGGTTATCCTCTGCTGGACTTGCAGAATCGTTACCGGCAACAAACCTACAGTCCAATGAGAGGTTAATGGTTATGCGGGAAGTCAATCCATTGCCAATGTCATTGTTATCTGGCTTTGCAATAAACCTGTGTTTAAAACTGGCGTATCAAATGGAAGAATCTTTGTTTTGCGGGCAGGTTGTACCTAATATTGCAATGGCTGAAACATATTGCAGGTTACTGCTCATTGCACCTCATTCACTATTCCGTTCTCATTTCAACCATTTGGCACAAAGGAGTCCTTCAGTATTGAGCAAGCCCGGCGTTACTCTTTTGGTGCTTGAAATTTTGAACTATCGTTTGCTTCCACTTTACAGGTACCAGGGAAAAAGCAAAGCCTTGATGTATGATGTAACAAAAATAATCTCTGCTCTGAAAGCAAAACGTGGAGATCATCGTGTATTTAGATTGGCCGAGAACTTGTGCATGAATCTTATTCTGTCACTGAGAGATTTCTTCTTAGTGAAAAGGGAAGGGAAGGGTCCAACTGAATTCACAGAAACTTTGAATCGAGTAACTGTGGTCACTCTTGCTATCATCATCAAGACAAGAGGAATTGCTGACGCTGATCATCTGCATTATCTTCAAACTATGTTAGAGCAGATATTAGTTTCTAGTAATCATACATGGTCAGAGAAAACACTACGCTATTTCCCTTCGCTTCTTCGTGATTTCTTGAATCTTCGGATAGATAACAGGGGCATTGCAATTCAAGCATGGCAGCAGGCAGAAACCACTGTGATTAACCAATGCAACCAGCTTCTGTCATCATCAGCTGATCCTACTTATGTTATGACCTATATAAGTCATAGTTTTCCTCAACACCGACAATATCTATGTGCTGGAGCATGGATCCTGATGCAAGGGCGCCCAGAGAACATAAATAGTGTAAACCTGGCACGTGTCTTGCGAGAGTTTTCCCCTGAGGAAGTAACTGCTAATATTTACATGATGGTGGATGTGCTACTCCATAACATTCAGTTAGAACATCAGCGCGGGCATTCCTTGCAGGACCTTCTAGTGAAAGCATGTGCAAACCTAACATTTTTTATTTGGACCCATGAGTTACTTCCTTTGGATATTATGCTTCTGGCACTTATTGATCGGGATGATGATCCCCTTGCTTTGCGTATTGTGATTAGTTTACTTGATAGGCAAGAACTTCAACAAAGAGTAAAACTATATTGTATGAATCGAGGCGCCCCTGAACACTGGCTTTACCCTGGAACCTTCAAACGAGTTGAATTGCAGACAGCCCTTGGAAATCATCTTTCTTGGAAGGATAGGTATCCCGCATTCTTCGATGACATTGCAGCGCGTTTGCTTCCAGTCATTCCACTAATAATTTATAGACTAATTGAAAATGATGCCGTAGATTCAGCGGACAGAGTTCTGGCCGTGTATACTCAATTTCTTGCTTACCACCCTTTTAGATTTACATTTGTTCGTGACATTCTTGCATATTTCTACGGCCATCTTCCCCCAAAGCTTATTGTTCGAATTCTGAATGTACTTGATATCAGCAAGATCCCATTGTCTGAGTCATTTCCGCAGCACATCAATTCATCAAATCCTGTCATCTGCCCACCTCCAGATTACTTTGCAACTCTCCTACTGGGAATTGTGAATAATGTTATACCTCCATTACATAACAACTCAAAATCTGGATCAGCTAGTGATGGTTTGAATAATTCAATGCGTGCTCCTCCTAACAAGACTCCAGCAACCTCTCAATCTGGGCAAACAAATGCTTCTGATGGCCAAAAAGCATTTTATCAAATTCAGGATCCAGGCACATATACTCAGCTGGTTCTTGAAACAGCAGTCATTGAATTACTGTCTCTTCCTGTATCTGCATCCCAGATTGTATCATCTCTGGTTCAAATTGTCGTCAATATACAACCGACGCTAATTCAATCCAGCAATGGCCTGCATGGAGCTACAAATGGTGTTGGGCAAGGCTCAGTGTTACCAACATCCCCTTCAGGTGGAAGCACAGATTCCTTGGGCACAAACAGATCAAGTCCTTCAGTTTCTGGAATAAATGTTTCTAGCTTTGTTTCTCGAAGTGGTTATACATGCCAACAATTGTCTTGCTTATTGATCCAAGCATGTGGCCATCTACTGGCTCAGCTCCCTCCTGATTTTCATAAACAACTCTATATAGAGGCATCACGTATAATAAAAGAAACATGGTGGCTCAGTGATGGCAAGAGATCACTGGGGGAACTAGACTCTGCTGTTGGTTATGCTTTGTTGGACCCAACATGGGCTGCTCAAGACAACACCTCAACAGCCATTGGTAATATTGTTGCTCTGCTTCATTCATTTTTCAGTAACCTCCCACTGGAATGGCTAGAAGGGACCCATCTTATCGTCAAACATCTCAGGCCAGTGACATCAGTTGCAATGTTGAGAATAGCATTCCGTATAATGGCTCCGTTGCTCCCAAAGCTTGCCAATGCTCACAACCTCTTCAATAAGACCCTCTCATTGATTTTCAATATGATGGCGGATGTCTTTGGGAAGAATGCTCAGCCATCAACTCCTGTTGAGCCACTAGAAGTGACGGATCTTATAGACTTCTTCCATCATATCGTCCACTATGAAGGACAAGGAGGGCCTGTGCAGGCTACCAGCAAGCCGAGACCAGAGGTTTTAGTTCTGTGTGGAAGAGCTGCCGAAAGTCTACGTCCAGAGATTCAGCATCTTCTTTCGCACTTAAAACCTGACACAAATTCTTCCATTTATGCTGCTACTCATCCTAAGTTGGCACAGAACACATCGTAA

Protein Analysis

1602

Amino Acids

179.49

Weight (kDa)

6.64

Isoelectric Point (pI)

47.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med23 PF11573 491 - 1211 6.9e-27 Mediator complex subunit 23
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1505, 1757
AarI CACCTGC 1 cut(s) 625
AasI GACNNNNNNGTC 1 cut(s) 1475
Acc36I ACCTGC 3 cut(s) 625, 2146, 2184
Acc65I GGTACC 1 cut(s) 2337
AccB1I GGYRCC 3 cut(s) 2337, 2886, 3218
AccB7I CCANNNNNTGG 2 cut(s) 1208, 4144
AccI GTMKAC 3 cut(s) 703, 3422, 4708
AccII CGCG 5 cut(s) 40, 66, 141, 3015, 3337
AcoI YGGCCR 5 cut(s) 2430, 3414, 3490, 3778, 4135
AcuI CTGAAG 5 cut(s) 1454, 1814, 2247, 3987, 4032
AcvI CACGTG 1 cut(s) 2921
AcyI GRCGYC 2 cut(s) 2887, 3219
AfaI GTAC 5 cut(s) 1191, 1291, 2163, 2339, 3531
AflII CTTAAG 1 cut(s) 1583
AflIII ACRYGT 2 cut(s) 1318, 2920
AjnI CCWGG 6 cut(s) 862, 1369, 2340, 2913, 3241, 3808
AjuI GAANNNNNNNTTGG 4 cut(s) 1234, 1266, 3985, 4017
Alw21I GWGCWC 2 cut(s) 365, 3724
Alw26I GTCTC 7 cut(s) 421, 552, 853, 1667, 1855, 3864, 4660
Alw44I GTGCAC 1 cut(s) 361
AlwNI CAGNNNCTG 3 cut(s) 281, 2782, 4055
ApaLI GTGCAC 1 cut(s) 361
ArsI GACNNNNNNTTYG 4 cut(s) 530, 562, 2015, 2047
Asp700I GAANNNNTTC 1 cut(s) 574
Asp718I GGTACC 1 cut(s) 2337
AspLEI GCGC 7 cut(s) 68, 178, 1954, 2889, 3015, 3221, 3337
AspS9I GGNCC 9 cut(s) 666, 1349, 1361, 2501, 3031, 3075, 4279, 4383, 4640
AsuC2I CCSGG 1 cut(s) 2280
AsuHPI GGTGA 4 cut(s) 606, 1154, 1161, 1293
AsuII TTCGAA 1 cut(s) 3517
AvaII GGWCC 7 cut(s) 1349, 1361, 2501, 3031, 3075, 4279, 4383
AxyI CCTNAGG 1 cut(s) 2943
BaeGI GKGCMC 2 cut(s) 365, 4031
BaeI ACNNNNGTAYC 2 cut(s) 2108, 2141
BalI TGGCCA 2 cut(s) 3780, 4137
BamHI GGATCC 2 cut(s) 2871, 3805
BanI GGYRCC 3 cut(s) 2337, 2886, 3218
BarI GAAGNNNNNNTAC 2 cut(s) 4078, 4110
BbrPI CACGTG 1 cut(s) 2921
Bbv12I GWGCWC 2 cut(s) 365, 3724
BbvCI CCTCAGC 1 cut(s) 31
BceAI ACGGC 4 cut(s) 382, 3374, 3401, 3505
BcgI CGANNNNNNTGC 2 cut(s) 3311, 3345
BciT130I CCWGG 6 cut(s) 864, 1371, 2342, 2915, 3243, 3810
BciVI GTATCC 2 cut(s) 1751, 3318
BclI TGATCA 2 cut(s) 1974, 2587
BcnI CCSGG 1 cut(s) 2280
BcoDI GTCTC 7 cut(s) 421, 552, 853, 1667, 1855, 3864, 4660
BfmI CTRYAG 2 cut(s) 657, 2027
BfoI RGCGCY 2 cut(s) 2890, 3222
BfrI CTTAAG 1 cut(s) 1583
BfuAI ACCTGC 3 cut(s) 625, 2146, 2184
BfuI GTATCC 2 cut(s) 1751, 3318
BlpI GCTNAGC 2 cut(s) 4149, 4554
Bme1390I CCNGG 7 cut(s) 864, 1371, 2280, 2342, 2915, 3243, 3810
Bme18I GGWCC 7 cut(s) 1349, 1361, 2501, 3031, 3075, 4279, 4383
BmgT120I GGNCC 9 cut(s) 666, 1349, 1361, 2501, 3031, 3075, 4279, 4383, 4640
BmrFI CCNGG 7 cut(s) 864, 1371, 2280, 2342, 2915, 3243, 3810
BmrI ACTGGG 5 cut(s) 391, 633, 833, 3644, 4250
BmsI GCATC 6 cut(s) 1648, 2868, 3376, 3884, 4199, 4735
BmuI ACTGGG 5 cut(s) 391, 633, 833, 3644, 4250
BpmI CTGGAG 4 cut(s) 218, 2883, 3594, 3723
Bpu10I CCTNAGC 1 cut(s) 31
Bpu1102I GCTNAGC 2 cut(s) 4149, 4554
Bpu14I TTCGAA 1 cut(s) 3517
BpuEI CTTGAG 2 cut(s) 1341, 4281
BpuMI CCSGG 1 cut(s) 2280
BsaAI YACGTR 3 cut(s) 1778, 2921, 4196
BsaBI GATNNNNATC 3 cut(s) 135, 3132, 3306
BsaHI GRCGYC 2 cut(s) 2887, 3219
BsaI GGTCTC 2 cut(s) 853, 4660
BsaJI CCNNGG 7 cut(s) 323, 863, 1369, 2341, 3241, 3277, 4023
BsaXI ACNNNNNCTCC 4 cut(s) 1247, 1277, 4629, 4659
Bse118I RCCGGY 1 cut(s) 2014
Bse21I CCTNAGG 1 cut(s) 2943
Bse8I GATNNNNATC 3 cut(s) 135, 3132, 3306
BseBI CCWGG 6 cut(s) 864, 1371, 2342, 2915, 3243, 3810
BseDI CCNNGG 7 cut(s) 323, 863, 1369, 2341, 3241, 3277, 4023
BseGI GGATG 9 cut(s) 907, 1912, 1929, 2983, 3133, 3875, 3995, 4540, 4780
BseJI GATNNNNATC 3 cut(s) 135, 3132, 3306
BseRI GAGGAG 2 cut(s) 186, 3714
BseSI GKGCMC 2 cut(s) 365, 4031
BseYI CCCAGC 1 cut(s) 581
BsgI GTGCAG 3 cut(s) 323, 328, 4667
Bsh1236I CGCG 5 cut(s) 40, 66, 141, 3015, 3337
BshNI GGYRCC 3 cut(s) 2337, 2886, 3218
BsiHKAI GWGCWC 2 cut(s) 365, 3724
BsiSI CCGG 2 cut(s) 2015, 2280
BslFI GGGAC 3 cut(s) 385, 1114, 4396
BsmAI GTCTC 7 cut(s) 421, 552, 853, 1667, 1855, 3864, 4660
BsmBI CGTCTC 1 cut(s) 552
BsmFI GGGAC 3 cut(s) 385, 1114, 4396
BsmI GAATGC 4 cut(s) 3019, 3314, 4442, 4555
Bso31I GGTCTC 2 cut(s) 853, 4660
Bsp119I TTCGAA 1 cut(s) 3517
Bsp1286I GDGCHC 3 cut(s) 365, 3724, 4031
Bsp1720I GCTNAGC 2 cut(s) 4149, 4554
Bsp68I TCGCGA 2 cut(s) 40, 141
BspFNI CGCG 5 cut(s) 40, 66, 141, 3015, 3337
BspMAI CTGCAG 1 cut(s) 661
BspMI ACCTGC 3 cut(s) 625, 2146, 2184
BspQI GCTCTTC 1 cut(s) 4687
BspT104I TTCGAA 1 cut(s) 3517
BspT107I GGYRCC 3 cut(s) 2337, 2886, 3218
BspTI CTTAAG 1 cut(s) 1583
BspTNI GGTCTC 2 cut(s) 853, 4660
BsrFI RCCGGY 1 cut(s) 2014
BssAI RCCGGY 1 cut(s) 2014
BssECI CCNNGG 7 cut(s) 323, 863, 1369, 2341, 3241, 3277, 4023
BssNAI GTATAC 1 cut(s) 3423
BssNI GRCGYC 2 cut(s) 2887, 3219
BssT1I CCWWGG 2 cut(s) 3277, 4023
Bst1107I GTATAC 1 cut(s) 3423
Bst2UI CCWGG 6 cut(s) 864, 1371, 2342, 2915, 3243, 3810
Bst4CI ACNGT 7 cut(s) 480, 562, 1972, 2031, 2539, 2758, 3858
Bst6I CTCTTC 4 cut(s) 89, 3867, 4499, 4687
BstACI GRCGYC 2 cut(s) 2887, 3219
BstAFI CTTAAG 1 cut(s) 1583
BstAPI GCANNNNNTGC 1 cut(s) 3025
BstBAI YACGTR 3 cut(s) 1778, 2921, 4196
BstBI TTCGAA 1 cut(s) 3517
BstDSI CCRYGG 1 cut(s) 323
BstENI CCTNNNNNAGG 1 cut(s) 1356
BstF5I GGATG 9 cut(s) 907, 1912, 1929, 2983, 3133, 3875, 3995, 4540, 4780
BstFNI CGCG 5 cut(s) 40, 66, 141, 3015, 3337
BstH2I RGCGCY 2 cut(s) 2890, 3222
BstHHI GCGC 7 cut(s) 68, 178, 1954, 2889, 3015, 3221, 3337
BstMAI GTCTC 7 cut(s) 421, 552, 853, 1667, 1855, 3864, 4660
BstNI CCWGG 6 cut(s) 864, 1371, 2342, 2915, 3243, 3810
BstNSI RCATGY 5 cut(s) 1283, 1868, 3051, 4101, 4134
BstSCI CCNGG 7 cut(s) 862, 1369, 2278, 2340, 2913, 3241, 3808
BstSFI CTRYAG 2 cut(s) 657, 2027
BstSLI GKGCMC 2 cut(s) 365, 4031
BstSNI TACGTA 1 cut(s) 1778
BstUI CGCG 5 cut(s) 40, 66, 141, 3015, 3337
BstX2I RGATCY 8 cut(s) 1026, 1216, 1686, 1720, 2871, 3546, 3805, 4591
BstXI CCANNNNNNTGG 2 cut(s) 4133, 4371
BstYI RGATCY 8 cut(s) 1026, 1216, 1686, 1720, 2871, 3546, 3805, 4591
BstZ17I GTATAC 1 cut(s) 3423
Bsu36I CCTNAGG 1 cut(s) 2943
BsuI GTATCC 2 cut(s) 1751, 3318
BtgI CCRYGG 1 cut(s) 323
BtsCI GGATG 9 cut(s) 907, 1912, 1929, 2983, 3133, 3875, 3995, 4540, 4780
BtsI GCAGTG 2 cut(s) 311, 1155
BtuMI TCGCGA 2 cut(s) 40, 141
BveI ACCTGC 3 cut(s) 625, 2146, 2184
CaiI CAGNNNCTG 3 cut(s) 281, 2782, 4055
CfoI GCGC 7 cut(s) 68, 178, 1954, 2889, 3015, 3221, 3337
Cfr10I RCCGGY 1 cut(s) 2014
Cfr13I GGNCC 9 cut(s) 666, 1349, 1361, 2501, 3031, 3075, 4279, 4383, 4640
CseI GACGC 3 cut(s) 534, 2591, 3934
Csp6I GTAC 5 cut(s) 1190, 1290, 2162, 2338, 3530
CviQI GTAC 5 cut(s) 1190, 1290, 2162, 2338, 3530
DinI GGCGCC 2 cut(s) 2888, 3220
DraI TTTAAA 2 cut(s) 753, 2115
DrdI GACNNNNNNGTC 1 cut(s) 1475
DseDI GACNNNNNNGTC 1 cut(s) 1475
EaeI YGGCCR 5 cut(s) 2430, 3414, 3490, 3778, 4135
Eam1104I CTCTTC 4 cut(s) 89, 3867, 4499, 4687
EarI CTCTTC 4 cut(s) 89, 3867, 4499, 4687
EciI GGCGGA 1 cut(s) 4547
Eco105I TACGTA 1 cut(s) 1778
Eco130I CCWWGG 2 cut(s) 3277, 4023
Eco31I GGTCTC 2 cut(s) 853, 4660
Eco32I GATATC 1 cut(s) 3538
Eco47I GGWCC 7 cut(s) 1349, 1361, 2501, 3031, 3075, 4279, 4383
Eco57I CTGAAG 5 cut(s) 1454, 1814, 2247, 3987, 4032
Eco72I CACGTG 1 cut(s) 2921
Eco81I CCTNAGG 1 cut(s) 2943
EcoNI CCTNNNNNAGG 1 cut(s) 1356
EcoO109I RGGNCCY 5 cut(s) 1349, 1361, 3031, 4383, 4640
EcoRI GAATTC 2 cut(s) 2510, 3519
EcoRII CCWGG 6 cut(s) 862, 1369, 2340, 2913, 3241, 3808
EcoRV GATATC 1 cut(s) 3538
EcoT14I CCWWGG 2 cut(s) 3277, 4023
EgeI GGCGCC 2 cut(s) 2888, 3220
EheI GGCGCC 2 cut(s) 2888, 3220
ErhI CCWWGG 2 cut(s) 3277, 4023
Esp3I CGTCTC 1 cut(s) 552
FalI AAGNNNNNCTT 4 cut(s) 1458, 1490, 2245, 2277
FaqI GGGAC 3 cut(s) 385, 1114, 4396
FauI CCCGC 4 cut(s) 2049, 2144, 3008, 3319
FbaI TGATCA 2 cut(s) 1974, 2587
FblI GTMKAC 3 cut(s) 703, 3422, 4708
FokI GGATG 9 cut(s) 914, 1919, 1936, 2990, 3140, 3862, 3982, 4547, 4767
GlaI GCGC 7 cut(s) 67, 177, 1953, 2888, 3014, 3220, 3336
GsaI CCCAGC 1 cut(s) 585
GsuI CTGGAG 4 cut(s) 218, 2883, 3594, 3723
HaeII RGCGCY 2 cut(s) 2890, 3222
HapII CCGG 2 cut(s) 2015, 2280
HgaI GACGC 3 cut(s) 534, 2591, 3934
HhaI GCGC 7 cut(s) 68, 178, 1954, 2889, 3015, 3221, 3337
Hin1I GRCGYC 2 cut(s) 2887, 3219
Hin6I GCGC 7 cut(s) 66, 176, 1952, 2887, 3013, 3219, 3335
HinP1I GCGC 7 cut(s) 66, 176, 1952, 2887, 3013, 3219, 3335
HincII GTYRAC 2 cut(s) 922, 1440
HindII GTYRAC 2 cut(s) 922, 1440
HindIII AAGCTT 4 cut(s) 1002, 1156, 3506, 4472
HpaII CCGG 2 cut(s) 2015, 2280
HphI GGTGA 4 cut(s) 606, 1154, 1161, 1293
Hpy99I CGWCG 3 cut(s) 25, 28, 3928
HpyCH4III ACNGT 7 cut(s) 480, 562, 1972, 2031, 2539, 2758, 3858
HpyCH4IV ACGT 7 cut(s) 706, 1318, 1777, 2404, 2920, 4195, 4711
HpySE526I ACGT 7 cut(s) 706, 1318, 1777, 2404, 2920, 4195, 4711
Hsp92I GRCGYC 2 cut(s) 2887, 3219
HspAI GCGC 7 cut(s) 66, 176, 1952, 2887, 3013, 3219, 3335
KasI GGCGCC 2 cut(s) 2886, 3218
KflI GGGWCCC 1 cut(s) 4383
KpnI GGTACC 1 cut(s) 2341
Ksp22I TGATCA 2 cut(s) 1974, 2587
LguI GCTCTTC 1 cut(s) 4687
LmnI GCTCC 8 cut(s) 237, 1339, 2864, 3727, 3956, 4158, 4462, 4470
LweI GCATC 6 cut(s) 1648, 2868, 3376, 3884, 4199, 4735
MaeII ACGT 7 cut(s) 706, 1318, 1777, 2404, 2920, 4195, 4711
MfeI CAATTG 4 cut(s) 1410, 1917, 1978, 4106
MflI RGATCY 8 cut(s) 1026, 1216, 1686, 1720, 2871, 3546, 3805, 4591
MhlI GDGCHC 3 cut(s) 365, 3724, 4031
MlsI TGGCCA 2 cut(s) 3780, 4137
MluNI TGGCCA 2 cut(s) 3780, 4137
Mly113I GGCGCC 2 cut(s) 2887, 3219
MlyI GAGTC 7 cut(s) 287, 601, 1096, 2266, 3569, 3730, 4247
MmeI TCCRAC 2 cut(s) 2528, 4257
Mox20I TGGCCA 2 cut(s) 3780, 4137
MroXI GAANNNNTTC 1 cut(s) 574
MscI TGGCCA 2 cut(s) 3780, 4137
MslI CAYNNNNRTG 3 cut(s) 1064, 2972, 4369
Msp20I TGGCCA 2 cut(s) 3780, 4137
MspA1I CMGCKG 3 cut(s) 2795, 3401, 3826
MspCI CTTAAG 1 cut(s) 1583
MspI CCGG 2 cut(s) 2015, 2280
MspR9I CCNGG 7 cut(s) 864, 1371, 2280, 2342, 2915, 3243, 3810
MunI CAATTG 4 cut(s) 1410, 1917, 1978, 4106
Mva1269I GAATGC 4 cut(s) 3019, 3314, 4442, 4555
MvaI CCWGG 6 cut(s) 864, 1371, 2342, 2915, 3243, 3810
MvnI CGCG 5 cut(s) 40, 66, 141, 3015, 3337
NarI GGCGCC 2 cut(s) 2887, 3219
NciI CCSGG 1 cut(s) 2280
NmeAIII GCCGAG 2 cut(s) 2458, 4689
NruI TCGCGA 2 cut(s) 40, 141
NspI RCATGY 5 cut(s) 1283, 1868, 3051, 4101, 4134
NspV TTCGAA 1 cut(s) 3517
PaeI GCATGC 1 cut(s) 1283
PaqCI CACCTGC 1 cut(s) 625
PciSI GCTCTTC 1 cut(s) 4687
PcsI WCGNNNNNNNCGW 1 cut(s) 2926
PctI GAATGC 4 cut(s) 3019, 3314, 4442, 4555
PdmI GAANNNNTTC 1 cut(s) 574
PflFI GACNNNGTC 2 cut(s) 277, 610
PflMI CCANNNNNTGG 2 cut(s) 1208, 4144
PleI GAGTC 7 cut(s) 286, 601, 1096, 2265, 3568, 3730, 4247
PluTI GGCGCC 2 cut(s) 2890, 3222
PmaCI CACGTG 1 cut(s) 2921
PmlI CACGTG 1 cut(s) 2921
PpsI GAGTC 7 cut(s) 286, 601, 1096, 2265, 3568, 3730, 4247
Ppu21I YACGTR 3 cut(s) 1778, 2921, 4196
PpuMI RGGWCCY 4 cut(s) 1349, 1361, 3031, 4383
PsiI TTATAA 2 cut(s) 1505, 1757
Psp5II RGGWCCY 4 cut(s) 1349, 1361, 3031, 4383
Psp6I CCWGG 6 cut(s) 862, 1369, 2340, 2913, 3241, 3808
PspCI CACGTG 1 cut(s) 2921
PspFI CCCAGC 1 cut(s) 581
PspGI CCWGG 6 cut(s) 862, 1369, 2340, 2913, 3241, 3808
PspPI GGNCC 9 cut(s) 666, 1349, 1361, 2501, 3031, 3075, 4279, 4383, 4640
PspPPI RGGWCCY 4 cut(s) 1349, 1361, 3031, 4383
PstI CTGCAG 1 cut(s) 661
PstNI CAGNNNCTG 3 cut(s) 281, 2782, 4055
PsuI RGATCY 8 cut(s) 1026, 1216, 1686, 1720, 2871, 3546, 3805, 4591
PsyI GACNNNGTC 2 cut(s) 277, 610
PvuII CAGCTG 2 cut(s) 2795, 3826
RruI TCGCGA 2 cut(s) 40, 141
RsaI GTAC 5 cut(s) 1191, 1291, 2163, 2339, 3531
RsaNI GTAC 5 cut(s) 1190, 1290, 2162, 2338, 3530
RseI CAYNNNNRTG 3 cut(s) 1064, 2972, 4369
SapI GCTCTTC 1 cut(s) 4687
Sau96I GGNCC 9 cut(s) 666, 1349, 1361, 2501, 3031, 3075, 4279, 4383, 4640
SchI GAGTC 7 cut(s) 287, 601, 1096, 2266, 3569, 3730, 4247
ScrFI CCNGG 7 cut(s) 864, 1371, 2280, 2342, 2915, 3243, 3810
SduI GDGCHC 3 cut(s) 365, 3724, 4031
SfaNI GCATC 6 cut(s) 1648, 2868, 3376, 3884, 4199, 4735
SfcI CTRYAG 2 cut(s) 657, 2027
SfoI GGCGCC 2 cut(s) 2888, 3220
SfuI TTCGAA 1 cut(s) 3517
SinI GGWCC 7 cut(s) 1349, 1361, 2501, 3031, 3075, 4279, 4383
SmiMI CAYNNNNRTG 3 cut(s) 1064, 2972, 4369
SmlI CTYRAG 3 cut(s) 1356, 1583, 4296
SmoI CTYRAG 3 cut(s) 1356, 1583, 4296
SnaBI TACGTA 1 cut(s) 1778
SphI GCATGC 1 cut(s) 1283
SspDI GGCGCC 2 cut(s) 2886, 3218
SspI AATATT 3 cut(s) 2170, 2962, 4327
StyD4I CCNGG 7 cut(s) 862, 1369, 2278, 2340, 2913, 3241, 3808
StyI CCWWGG 2 cut(s) 3277, 4023
TaaI ACNGT 7 cut(s) 480, 562, 1972, 2031, 2539, 2758, 3858
TaiI ACGT 7 cut(s) 709, 1321, 1780, 2407, 2923, 4198, 4714
TatI WGTACW 3 cut(s) 1189, 1289, 3529
TauI GCSGC 1 cut(s) 1515
TspGWI ACGGA 5 cut(s) 660, 2216, 4436, 4449, 4604
Tth111I GACNNNGTC 2 cut(s) 277, 610
Van91I CCANNNNNTGG 2 cut(s) 1208, 4144
Vha464I CTTAAG 1 cut(s) 1583
VneI GTGCAC 1 cut(s) 361
VpaK11BI GGWCC 7 cut(s) 1349, 1361, 2501, 3031, 3075, 4279, 4383
XagI CCTNNNNNAGG 1 cut(s) 1356
XceI RCATGY 5 cut(s) 1283, 1868, 3051, 4101, 4134
XcmI CCANNNNNNNNNTGG 1 cut(s) 1305
XmiI GTMKAC 3 cut(s) 703, 3422, 4708
XmnI GAANNNNTTC 1 cut(s) 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.