RchiOBHm_Chr4g0443371

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0009426
Physical Location & Seq
Reverse (-)
64524413 .. 64534581
10169 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41107

Sequence Viewer

Length: 4809 bp
ATGGATCAAAACCAGAGATCGTCGTCGGCCTCAGCTTCGCGAGCTTACCAGTTCCACCCTGCTCGCGCTGCAATCGTCAATCTCTTCGACCTCTACTTAGGAGGAAAGAGCAGTAGTCGCCAGAAATCAGAGGATTCAAATCGCGAACCTCCGAACAAGTCACAGAAGCGTGTGCTTGCGCTCAATAGAGAACTTCCTCCTCGAAACGAGCAGTTCATTCTCGATTTCGAGCAGCTCCAGAGCCAGTTTCCTGACCAAGATCAACTGCGTGTTGTGACAGAGTCTGTGCTTATATCTCTAGTTGTGCAGTGCAGTAATCATGCACCACGGGCAGAGTTTCTTCTCTTTGCTTTAAGGAGTTTGTGCACAATAGGTCACATTAACTGGGATAGTTTTTTGCCGTCCCTTCTTTCTTCTGTCTCCACGGCAGAAATGTCAATGGGTCAAGGGAGTCAAGCAATGACGGGTATTTCATCACAGTCCAGTATGCTTCCAAGTTCAAATGCAATTCCCAATGCATCTAACTTTCAATCTTCAAATCCTGCCTCTCCATTACCTTCTGTTCATGGAATTGGTTCCCCCAGCCAGACAGCTATTGAACCTATGACTCTGTCACCTGCTAAATCATCTGATATGTCCAGTAATGGACAGCAGGCTGCAGCAAGGGCCAATTCATCTATAAGAGATAATGCAATCAGCAGTCTACGTCAGTTGTGTTGCAAAATTATTTTGACTGGACTGGAATTTAATTTAAAACCAGCAACTCATGCGGACATATTTTCTCATATGCTGAATTGGCTGGTTAATTGGGATCAAAAACAACTGGGGGCTGATGAATCTGATGGCATAAAGTCTTGGAGACCTGGGAAGGCACTAATTGAATGGCTGCATAGCTGTTTGGATGTCATTTGGCTTTTGGTTGACGAGGATAAATGCCGAGTTCCCTTCTATGAATTACTGCGCAGTGGCTTGCAATTCATGGAGAACATACCTGATGATGAAGCTTTGTTTACTCTAATCTTGGAGATCCATAGGAGGCGAGATATGATGGCTATGCACATGAAAATGCTAGATCAACACTTACATTGCCCTACATTTGGGACTCATCGAATTTTCTCGCAGACTACTCCTAGTATTTCAGGTGAAGCAGTGGCAAGCTTGCGGTATTCACCAATCACATATCCAAGTGTACTTGGGGAACCATTGCATGGAGAGGATCTTGCAATTTCTATTCCAAAAGGAAGTTTAGATTGGGAGAGAGCGTTGCGTTGCATAAGGCATGCTATTTGTACTACTCCATCACCTGACTGGTGGAAACGTGTGCTGCTTGTGGCTCCTTGTTATAGGGGTCCTTCTCAAGGACCTACTCCTGGTGCTGTTTTCACTTCTGAAATGATTTGTGAGGCAACAATTGATAGAATTGTTGAGCTACTGAAGTTGACAAATTCAGATGTTAATTGCTGGCAAGACTGGCTTGTCTTTTCAGATATATTCTTTTTTCTCATAAAAAGCGGCTGTGTTGATTTCGTCTATTTTGTGGACAAGCTAGTTTCACGCCTTACAGAGAGTGATCCCCATATTCTTAAGACCAATCATGTCACTTGGCTTCTAGCTCAAATTATTCGGGTTGAGCTTGTGATAAATGCTCTAAATTCAGATGCTAGAAAGGTAGAGACAACTCGGAAGATCCTATCGCTTCATAAAGAAGATAGAAGCTCAGATCCAAATAGTCCTCAGAGTATCCTGCTTGACTTTATAAGCAGTTGCCAAAATTTACGTATTTGGTCATTGAATACAACAACTAGAGAATATCTGAATAATGAGCAGCTTCAGAAAGGAAAGCAAATAGATGAATGGTGGAGACATGCAAGCAAAGGGGATCGTATAATGGATTATATGAATATGGATGATAGGTCAATTGGGATGTTTTGGGTTGTATCTTACACTATGGCGCAACCAGCTTGTGAAACTGTGATCAATTGGTTAACCTCTGCTGGAGTTGCAGAATCGTTACCGGCAACAAACCTACAGTCCAATGAGAGGTTAATGGTTATGCGGGAAGTCAATCCATTGCCAATGTCATTGTTATCTGGCTTTGCAATAAACCTGTGTTTAAAACTGGCGTATCAAATGGAAGAATCTTTGTTTTGCGGGCAGGTTGTACCTAATATTGCAATGGCTGAAACATATTGCAGGTTACTGCTCATTGCACCTCATTCACTATTCCGTTCTCATTTCAATCATTTGGCACAAAGGAGTCCTTCAGTATTGAGCAAGCCCGGCGTTACTCTTTTGGTGCTTGAAATTTTGAACTATCGTTTGCTTCCACTTTACAGGTACCAGGGAAAAAGCAAAGCCTTGATGTATGATGTAACAAAAATAATCTCTGCTCTGAAAGCAAAACGTGGAGACCATCGTGTATTTAGATTGGCCGAGAACTTGTGCATGAATCTTATTCTGTCACTGAGAGATTTCTTCTTAGTGAAAAGGGAAGGGAAGGGTCCAACTGAATTCACAGAAACTTTGAATCGAGTAACTGTGGTCACTCTTGCTATCATCATCAAGACAAGAGGAATTGCTGATGCTGATCATCTGCATTATCTTCAAACTATGTTAGAGCAGATATTAGTTTCTAGTAACCATACATGGTCAGAGAAAACACTACGCTATTTCCCTTCGCTTCTTCGTGATTTCTTGATTCCTCGGATAGATAACAGGGGCATTGCAATTCAAGCATGGCAGCAGGCAGAAACCACTGTGATCAACCAATGCAACCAGCTTCTGTCATCATCAGCTGATCCTACTTATGTTATGACCTATATAAGTCATAGTTTTCCTCAACACCGACAATATCTATGTGCTGGAGCATGGATCCTGATGCAAGGGCGCCCAGAGAACATAAATAGTGTAAACCTGGCACGTGTCTTGCGAGAGTTTTCCCCTGAGGAAGTAACTGCTAATATTTACATGATGGTGGATGTGCTACTCCATAACATTCAGTTAGAACATCAGCGCGGGCATTCCTTGCAGGACCTTCTAGTGAAAGCATGTGCAAACCTAACATTTTTTATTTGGACCCATGAGTTACTTCCTTTGGATATTATGCTTCTGGCACTTATTGATCGGGATGATGATCCCCATGCTTTGCGTATTGTGATTAGTTTACTTGATAGGCAAGAACTTCAACAAAGAGTAAAACTATATTGTATGAATCGAGGCGCCCCTGAACACTGGCTTTACCCTGGACCCTTCAAACGAGTTGAATTGCAGACAGCCCTTGGAAATCATCTTTCTTGGAAGGATAGGTATCCTGCATTCTTCGATGACATTGCAGCGCGTTTGCTTCCGGTCATTCCACTAATAATTTATAGACTAATTGAAAATGATGCCATAGATTCAGCGGACAGAGTTCTGGCCATGTATACTCAATTTCTTGCTTACCACCCTTTTAGATTTACATTTGTTCGTGACATTCTTGCATATTTCTACGGCCTTCTTCCCCCAAAGCTTATTGTTCGAATTCTGAATGTACTTGATATCAGCAAGATCCCATTGTCTGAGTCATTTCCGCAGCACATCAATTCATCAAATCCTGTCATCTGCCCACCTCCAGATTACTTTGCAACTCTCCTACTGGGAATAGTGAATAATGTTATACCTCCATTACATAACAACTCAAAATCTGGATCAGCTAGTGATGGTTTAAATAGTTCAATGCGTGCTCCACCTAACAAGACTCCAGCAACCTCTCAATCTGGGCAAACAAATGCTTCTGATGGCCAAAAAGCATTTTATCAAATTCAGGATCCAGGCACATATACTCAGCTGGTTCTTGAAACAGCAGTCATTGAATTACTGTCTCTTCCGGTATCTGCATCCCAGATTGTATCATCTCTGGTTCAAATTGTCGTCAATATACAACCGACGCTAATTCAATCCAGCAATGGCCTGCATGGAGCTACAAATGGTGTTGGGCAAGGCTCAGTGTTACCAACATCCCCTTCAGGTGGAAGCACAGATTCCTTGGGCACAAACAGATCAAGTCCTTCAGTTTCTGGAATAAATGTTTCTAGCTTTGTTTCTCGAAGTGGTTATACATGCCAACAATTGTCTTGCTTATTGATCCAAGCATGTGGCCATCTACTGGCTCAGCTCCCTCCCGATTTTCATAAACAACTCTATATGGAGGCATCACGTATAATAAAAGAAACATGGTGGCTCAGTGATGGCAAGAGATCACTAGGGGAACTAGACTCTGCTGTTGGTTATGCTTTGTTGGACCCAACATGGGCTGCTCAAGACAACACCTCAACAGCCATTGGTAATATTGTTGCTCTGCTTCATTCATTTTTCAGTAACCTCCCACTGGAATGGCTAGAAGGGACCCATCTTATCGTCAAACATCTCAGGCCAGTGACATCAGTTGCAATGTTGAGAATAGCATTCCGTATAATGGCTCCGTTGCTCCCAAAGCTTGCCAATGCTCACAACCTCTTCAATAAGACCCTCTCATTGATTTTCAATATGATGGCGGATGTCTTTGGGAAGAATGCTCAGCCATCAACTCCTGTTGAGCCACTAGAAGTGACAGATCTTATAGACTTCTTCCATCATATCGTCCACTATGAAGGACAAGGAGGGCCTGTGCAGGCTCCCAGCAAGCCGAGACCAGAGGTTTTAGTTCTGTGTGGAAGAGCTGCCGAAAGTCTACGTCCAGAGATTCAGCATCTTCTTTCGCACTTAAAACCCGACACAAATTCTTCCATTTATGCTGCTACTCATCCTAAGTTGGCACAGAACACATCGTAA

Protein Analysis

1602

Amino Acids

179.4

Weight (kDa)

6.7

Isoelectric Point (pI)

48.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med23 PF11573 491 - 1165 8.2e-26 Mediator complex subunit 23
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1757
AarI CACCTGC 1 cut(s) 625
AasI GACNNNNNNGTC 1 cut(s) 1475
Acc16I TGCGCA 1 cut(s) 962
Acc36I ACCTGC 3 cut(s) 625, 2146, 2184
Acc65I GGTACC 1 cut(s) 2337
AccB1I GGYRCC 3 cut(s) 2337, 2886, 3218
AccB7I CCANNNNNTGG 2 cut(s) 1208, 4144
AccI GTMKAC 3 cut(s) 703, 3422, 4708
AccII CGCG 5 cut(s) 40, 66, 144, 3015, 3337
AciI CCGC 9 cut(s) 770, 1162, 1512, 2056, 2151, 3015, 3401, 3569, 4532
AcoI YGGCCR 4 cut(s) 2430, 3414, 3778, 4135
AcuI CTGAAG 5 cut(s) 1454, 1814, 2247, 3987, 4032
AcvI CACGTG 1 cut(s) 2921
AcyI GRCGYC 2 cut(s) 2887, 3219
AfaI GTAC 5 cut(s) 1191, 1291, 2163, 2339, 3531
AflII CTTAAG 1 cut(s) 1583
AflIII ACRYGT 2 cut(s) 1318, 2920
AjnI CCWGG 6 cut(s) 862, 1369, 2340, 2913, 3241, 3808
AjuI GAANNNNNNNTTGG 4 cut(s) 1234, 1266, 3985, 4017
Alw21I GWGCWC 2 cut(s) 368, 3724
Alw26I GTCTC 7 cut(s) 424, 853, 1667, 1855, 2403, 3864, 4660
Alw44I GTGCAC 1 cut(s) 364
AlwNI CAGNNNCTG 3 cut(s) 284, 2782, 4055
ApaLI GTGCAC 1 cut(s) 364
ArsI GACNNNNNNTTYG 2 cut(s) 2015, 2047
Asp700I GAANNNNTTC 1 cut(s) 574
Asp718I GGTACC 1 cut(s) 2337
AspLEI GCGC 8 cut(s) 68, 181, 963, 1954, 2889, 3015, 3221, 3337
AsuC2I CCSGG 1 cut(s) 2280
AsuHPI GGTGA 4 cut(s) 606, 1154, 1161, 1293
AsuII TTCGAA 1 cut(s) 3517
AvaII GGWCC 8 cut(s) 1349, 1361, 2501, 3031, 3075, 3245, 4279, 4383
AxyI CCTNAGG 1 cut(s) 2943
BaeGI GKGCMC 2 cut(s) 368, 4031
BaeI ACNNNNGTAYC 2 cut(s) 2108, 2141
BalI TGGCCA 3 cut(s) 3416, 3780, 4137
BamHI GGATCC 2 cut(s) 2871, 3805
BanI GGYRCC 3 cut(s) 2337, 2886, 3218
BarI GAAGNNNNNNTAC 2 cut(s) 4078, 4110
BbrPI CACGTG 1 cut(s) 2921
Bbv12I GWGCWC 2 cut(s) 368, 3724
BbvCI CCTCAGC 1 cut(s) 31
BceAI ACGGC 3 cut(s) 385, 441, 3505
BcgI CGANNNNNNTGC 2 cut(s) 3311, 3345
BciT130I CCWGG 6 cut(s) 864, 1371, 2342, 2915, 3243, 3810
BciVI GTATCC 2 cut(s) 1751, 3318
BclI TGATCA 3 cut(s) 1974, 2587, 2760
BcnI CCSGG 1 cut(s) 2280
BcoDI GTCTC 7 cut(s) 424, 853, 1667, 1855, 2403, 3864, 4660
BfmI CTRYAG 2 cut(s) 657, 2027
BfoI RGCGCY 2 cut(s) 2890, 3222
BfrI CTTAAG 1 cut(s) 1583
BfuAI ACCTGC 3 cut(s) 625, 2146, 2184
BfuI GTATCC 2 cut(s) 1751, 3318
BglII AGATCT 1 cut(s) 4591
BlpI GCTNAGC 2 cut(s) 4149, 4554
Bme1390I CCNGG 7 cut(s) 864, 1371, 2280, 2342, 2915, 3243, 3810
Bme18I GGWCC 8 cut(s) 1349, 1361, 2501, 3031, 3075, 3245, 4279, 4383
BmrFI CCNGG 7 cut(s) 864, 1371, 2280, 2342, 2915, 3243, 3810
BmrI ACTGGG 3 cut(s) 394, 833, 3644
BmsI GCATC 8 cut(s) 527, 1648, 2572, 2868, 3376, 3884, 4199, 4735
BmuI ACTGGG 3 cut(s) 394, 833, 3644
BpmI CTGGAG 5 cut(s) 221, 2016, 2883, 3594, 3723
Bpu10I CCTNAGC 1 cut(s) 31
Bpu1102I GCTNAGC 2 cut(s) 4149, 4554
Bpu14I TTCGAA 1 cut(s) 3517
BpuEI CTTGAG 2 cut(s) 1341, 4281
BpuMI CCSGG 1 cut(s) 2280
BsaAI YACGTR 3 cut(s) 1778, 2921, 4196
BsaBI GATNNNNATC 4 cut(s) 138, 2586, 3132, 3306
BsaHI GRCGYC 2 cut(s) 2887, 3219
BsaI GGTCTC 3 cut(s) 853, 2403, 4660
BsaJI CCNNGG 8 cut(s) 326, 423, 863, 2341, 2702, 3241, 3277, 4023
BsaWI WCCGGW 2 cut(s) 3346, 3865
BsaXI ACNNNNNCTCC 4 cut(s) 1247, 1277, 4629, 4659
Bse118I RCCGGY 1 cut(s) 2014
Bse21I CCTNAGG 1 cut(s) 2943
Bse8I GATNNNNATC 4 cut(s) 138, 2586, 3132, 3306
BseBI CCWGG 6 cut(s) 864, 1371, 2342, 2915, 3243, 3810
BseDI CCNNGG 8 cut(s) 326, 423, 863, 2341, 2702, 3241, 3277, 4023
BseGI GGATG 9 cut(s) 907, 1912, 1929, 2983, 3133, 3875, 3995, 4540, 4780
BseJI GATNNNNATC 4 cut(s) 138, 2586, 3132, 3306
BseRI GAGGAG 1 cut(s) 189
BseSI GKGCMC 2 cut(s) 368, 4031
BseYI CCCAGC 2 cut(s) 581, 4655
BsgI GTGCAG 3 cut(s) 326, 331, 4667
Bsh1236I CGCG 5 cut(s) 40, 66, 144, 3015, 3337
BshNI GGYRCC 3 cut(s) 2337, 2886, 3218
BsiHKAI GWGCWC 2 cut(s) 368, 3724
BsiSI CCGG 4 cut(s) 2015, 2280, 3347, 3866
BslFI GGGAC 3 cut(s) 388, 1114, 4396
BsmAI GTCTC 7 cut(s) 424, 853, 1667, 1855, 2403, 3864, 4660
BsmFI GGGAC 3 cut(s) 388, 1114, 4396
BsmI GAATGC 4 cut(s) 3019, 3314, 4442, 4555
Bso31I GGTCTC 3 cut(s) 853, 2403, 4660
Bsp119I TTCGAA 1 cut(s) 3517
Bsp1286I GDGCHC 3 cut(s) 368, 3724, 4031
Bsp1720I GCTNAGC 2 cut(s) 4149, 4554
Bsp68I TCGCGA 2 cut(s) 40, 144
BspACI CCGC 9 cut(s) 770, 1162, 1512, 2056, 2151, 3015, 3401, 3569, 4532
BspFNI CGCG 5 cut(s) 40, 66, 144, 3015, 3337
BspMAI CTGCAG 1 cut(s) 661
BspMI ACCTGC 3 cut(s) 625, 2146, 2184
BspQI GCTCTTC 1 cut(s) 4687
BspT104I TTCGAA 1 cut(s) 3517
BspT107I GGYRCC 3 cut(s) 2337, 2886, 3218
BspTI CTTAAG 1 cut(s) 1583
BspTNI GGTCTC 3 cut(s) 853, 2403, 4660
BsrFI RCCGGY 1 cut(s) 2014
BssAI RCCGGY 1 cut(s) 2014
BssECI CCNNGG 8 cut(s) 326, 423, 863, 2341, 2702, 3241, 3277, 4023
BssNAI GTATAC 1 cut(s) 3423
BssNI GRCGYC 2 cut(s) 2887, 3219
BssT1I CCWWGG 2 cut(s) 3277, 4023
Bst1107I GTATAC 1 cut(s) 3423
Bst2UI CCWGG 6 cut(s) 864, 1371, 2342, 2915, 3243, 3810
Bst4CI ACNGT 6 cut(s) 480, 1972, 2031, 2539, 2758, 3858
Bst6I CTCTTC 4 cut(s) 89, 3867, 4499, 4687
BstACI GRCGYC 2 cut(s) 2887, 3219
BstAFI CTTAAG 1 cut(s) 1583
BstAPI GCANNNNNTGC 2 cut(s) 767, 3025
BstBAI YACGTR 3 cut(s) 1778, 2921, 4196
BstBI TTCGAA 1 cut(s) 3517
BstDSI CCRYGG 2 cut(s) 326, 423
BstENI CCTNNNNNAGG 1 cut(s) 1356
BstF5I GGATG 9 cut(s) 907, 1912, 1929, 2983, 3133, 3875, 3995, 4540, 4780
BstFNI CGCG 5 cut(s) 40, 66, 144, 3015, 3337
BstH2I RGCGCY 2 cut(s) 2890, 3222
BstHHI GCGC 8 cut(s) 68, 181, 963, 1954, 2889, 3015, 3221, 3337
BstMAI GTCTC 7 cut(s) 424, 853, 1667, 1855, 2403, 3864, 4660
BstNI CCWGG 6 cut(s) 864, 1371, 2342, 2915, 3243, 3810
BstNSI RCATGY 5 cut(s) 1283, 1868, 3051, 4101, 4134
BstSCI CCNGG 7 cut(s) 862, 1369, 2278, 2340, 2913, 3241, 3808
BstSFI CTRYAG 2 cut(s) 657, 2027
BstSLI GKGCMC 2 cut(s) 368, 4031
BstSNI TACGTA 1 cut(s) 1778
BstUI CGCG 5 cut(s) 40, 66, 144, 3015, 3337
BstX2I RGATCY 8 cut(s) 1026, 1216, 1686, 1720, 2871, 3546, 3805, 4591
BstXI CCANNNNNNTGG 2 cut(s) 4133, 4371
BstYI RGATCY 8 cut(s) 1026, 1216, 1686, 1720, 2871, 3546, 3805, 4591
BstZ17I GTATAC 1 cut(s) 3423
Bsu36I CCTNAGG 1 cut(s) 2943
BsuI GTATCC 2 cut(s) 1751, 3318
BtgI CCRYGG 2 cut(s) 326, 423
BtsCI GGATG 9 cut(s) 907, 1912, 1929, 2983, 3133, 3875, 3995, 4540, 4780
BtsI GCAGTG 3 cut(s) 314, 970, 1155
BtuMI TCGCGA 2 cut(s) 40, 144
BveI ACCTGC 3 cut(s) 625, 2146, 2184
CaiI CAGNNNCTG 3 cut(s) 284, 2782, 4055
CfoI GCGC 8 cut(s) 68, 181, 963, 1954, 2889, 3015, 3221, 3337
Cfr10I RCCGGY 1 cut(s) 2014
CseI GACGC 1 cut(s) 3934
Csp6I GTAC 5 cut(s) 1190, 1290, 2162, 2338, 3530
CviQI GTAC 5 cut(s) 1190, 1290, 2162, 2338, 3530
DinI GGCGCC 2 cut(s) 2888, 3220
DraI TTTAAA 3 cut(s) 753, 2115, 3705
DrdI GACNNNNNNGTC 1 cut(s) 1475
DseDI GACNNNNNNGTC 1 cut(s) 1475
EaeI YGGCCR 4 cut(s) 2430, 3414, 3778, 4135
Eam1104I CTCTTC 4 cut(s) 89, 3867, 4499, 4687
EarI CTCTTC 4 cut(s) 89, 3867, 4499, 4687
EciI GGCGGA 1 cut(s) 4547
Eco105I TACGTA 1 cut(s) 1778
Eco130I CCWWGG 2 cut(s) 3277, 4023
Eco31I GGTCTC 3 cut(s) 853, 2403, 4660
Eco32I GATATC 1 cut(s) 3538
Eco47I GGWCC 8 cut(s) 1349, 1361, 2501, 3031, 3075, 3245, 4279, 4383
Eco57I CTGAAG 5 cut(s) 1454, 1814, 2247, 3987, 4032
Eco72I CACGTG 1 cut(s) 2921
Eco81I CCTNAGG 1 cut(s) 2943
EcoNI CCTNNNNNAGG 1 cut(s) 1356
EcoO109I RGGNCCY 5 cut(s) 1349, 1361, 3031, 4383, 4640
EcoRI GAATTC 2 cut(s) 2510, 3519
EcoRII CCWGG 6 cut(s) 862, 1369, 2340, 2913, 3241, 3808
EcoRV GATATC 1 cut(s) 3538
EcoT14I CCWWGG 2 cut(s) 3277, 4023
EcoT22I ATGCAT 1 cut(s) 520
EgeI GGCGCC 2 cut(s) 2888, 3220
EheI GGCGCC 2 cut(s) 2888, 3220
ErhI CCWWGG 2 cut(s) 3277, 4023
FalI AAGNNNNNCTT 4 cut(s) 1458, 1490, 2245, 2277
FaqI GGGAC 3 cut(s) 388, 1114, 4396
FauI CCCGC 3 cut(s) 2049, 2144, 3008
FauNDI CATATG 1 cut(s) 786
FbaI TGATCA 3 cut(s) 1974, 2587, 2760
FblI GTMKAC 3 cut(s) 703, 3422, 4708
FokI GGATG 9 cut(s) 914, 1919, 1936, 2990, 3140, 3862, 3982, 4547, 4767
FspI TGCGCA 1 cut(s) 962
GlaI GCGC 8 cut(s) 67, 180, 962, 1953, 2888, 3014, 3220, 3336
GsaI CCCAGC 2 cut(s) 585, 4659
GsuI CTGGAG 5 cut(s) 221, 2016, 2883, 3594, 3723
HaeII RGCGCY 2 cut(s) 2890, 3222
HapII CCGG 4 cut(s) 2015, 2280, 3347, 3866
HgaI GACGC 1 cut(s) 3934
HhaI GCGC 8 cut(s) 68, 181, 963, 1954, 2889, 3015, 3221, 3337
Hin1I GRCGYC 2 cut(s) 2887, 3219
Hin6I GCGC 8 cut(s) 66, 179, 961, 1952, 2887, 3013, 3219, 3335
HinP1I GCGC 8 cut(s) 66, 179, 961, 1952, 2887, 3013, 3219, 3335
HincII GTYRAC 3 cut(s) 922, 1440, 1986
HindII GTYRAC 3 cut(s) 922, 1440, 1986
HindIII AAGCTT 4 cut(s) 1002, 1156, 3506, 4472
HpaI GTTAAC 1 cut(s) 1986
HpaII CCGG 4 cut(s) 2015, 2280, 3347, 3866
HphI GGTGA 4 cut(s) 606, 1154, 1161, 1293
Hpy99I CGWCG 3 cut(s) 25, 28, 3928
HpyCH4III ACNGT 6 cut(s) 480, 1972, 2031, 2539, 2758, 3858
HpyCH4IV ACGT 7 cut(s) 706, 1318, 1777, 2404, 2920, 4195, 4711
HpySE526I ACGT 7 cut(s) 706, 1318, 1777, 2404, 2920, 4195, 4711
Hsp92I GRCGYC 2 cut(s) 2887, 3219
HspAI GCGC 8 cut(s) 66, 179, 961, 1952, 2887, 3013, 3219, 3335
KasI GGCGCC 2 cut(s) 2886, 3218
KflI GGGWCCC 1 cut(s) 4383
KpnI GGTACC 1 cut(s) 2341
Ksp22I TGATCA 3 cut(s) 1974, 2587, 2760
KspAI GTTAAC 1 cut(s) 1986
LguI GCTCTTC 1 cut(s) 4687
LmnI GCTCC 9 cut(s) 240, 1339, 2864, 3727, 3956, 4158, 4462, 4470, 4657
LweI GCATC 8 cut(s) 527, 1648, 2572, 2868, 3376, 3884, 4199, 4735
MaeII ACGT 7 cut(s) 706, 1318, 1777, 2404, 2920, 4195, 4711
MfeI CAATTG 4 cut(s) 1410, 1917, 1978, 4106
MflI RGATCY 8 cut(s) 1026, 1216, 1686, 1720, 2871, 3546, 3805, 4591
MhlI GDGCHC 3 cut(s) 368, 3724, 4031
MlsI TGGCCA 3 cut(s) 3416, 3780, 4137
MluNI TGGCCA 3 cut(s) 3416, 3780, 4137
Mly113I GGCGCC 2 cut(s) 2887, 3219
MlyI GAGTC 8 cut(s) 290, 460, 601, 1096, 2266, 3569, 3730, 4247
MmeI TCCRAC 2 cut(s) 2528, 4257
Mox20I TGGCCA 3 cut(s) 3416, 3780, 4137
Mph1103I ATGCAT 1 cut(s) 520
MroXI GAANNNNTTC 1 cut(s) 574
MscI TGGCCA 3 cut(s) 3416, 3780, 4137
MslI CAYNNNNRTG 3 cut(s) 1064, 2972, 4369
Msp20I TGGCCA 3 cut(s) 3416, 3780, 4137
MspA1I CMGCKG 3 cut(s) 2795, 3401, 3826
MspCI CTTAAG 1 cut(s) 1583
MspI CCGG 4 cut(s) 2015, 2280, 3347, 3866
MspR9I CCNGG 7 cut(s) 864, 1371, 2280, 2342, 2915, 3243, 3810
MunI CAATTG 4 cut(s) 1410, 1917, 1978, 4106
Mva1269I GAATGC 4 cut(s) 3019, 3314, 4442, 4555
MvaI CCWGG 6 cut(s) 864, 1371, 2342, 2915, 3243, 3810
MvnI CGCG 5 cut(s) 40, 66, 144, 3015, 3337
NarI GGCGCC 2 cut(s) 2887, 3219
NciI CCSGG 1 cut(s) 2280
NdeI CATATG 1 cut(s) 786
NmeAIII GCCGAG 3 cut(s) 962, 2458, 4689
NruI TCGCGA 2 cut(s) 40, 144
NsbI TGCGCA 1 cut(s) 962
NsiI ATGCAT 1 cut(s) 520
NspI RCATGY 5 cut(s) 1283, 1868, 3051, 4101, 4134
NspV TTCGAA 1 cut(s) 3517
PaeI GCATGC 1 cut(s) 1283
PaqCI CACCTGC 1 cut(s) 625
PciSI GCTCTTC 1 cut(s) 4687
PcsI WCGNNNNNNNCGW 1 cut(s) 2926
PctI GAATGC 4 cut(s) 3019, 3314, 4442, 4555
PdmI GAANNNNTTC 1 cut(s) 574
PflFI GACNNNGTC 2 cut(s) 280, 610
PflMI CCANNNNNTGG 2 cut(s) 1208, 4144
PleI GAGTC 8 cut(s) 289, 459, 601, 1096, 2265, 3568, 3730, 4247
PluTI GGCGCC 2 cut(s) 2890, 3222
PmaCI CACGTG 1 cut(s) 2921
PmlI CACGTG 1 cut(s) 2921
PpsI GAGTC 8 cut(s) 289, 459, 601, 1096, 2265, 3568, 3730, 4247
Ppu21I YACGTR 3 cut(s) 1778, 2921, 4196
PpuMI RGGWCCY 4 cut(s) 1349, 1361, 3031, 4383
PsiI TTATAA 1 cut(s) 1757
Psp5II RGGWCCY 4 cut(s) 1349, 1361, 3031, 4383
Psp6I CCWGG 6 cut(s) 862, 1369, 2340, 2913, 3241, 3808
PspCI CACGTG 1 cut(s) 2921
PspFI CCCAGC 2 cut(s) 581, 4655
PspGI CCWGG 6 cut(s) 862, 1369, 2340, 2913, 3241, 3808
PspPPI RGGWCCY 4 cut(s) 1349, 1361, 3031, 4383
PstI CTGCAG 1 cut(s) 661
PstNI CAGNNNCTG 3 cut(s) 284, 2782, 4055
PsuI RGATCY 8 cut(s) 1026, 1216, 1686, 1720, 2871, 3546, 3805, 4591
PsyI GACNNNGTC 2 cut(s) 280, 610
PvuII CAGCTG 2 cut(s) 2795, 3826
RruI TCGCGA 2 cut(s) 40, 144
RsaI GTAC 5 cut(s) 1191, 1291, 2163, 2339, 3531
RsaNI GTAC 5 cut(s) 1190, 1290, 2162, 2338, 3530
RseI CAYNNNNRTG 3 cut(s) 1064, 2972, 4369
SapI GCTCTTC 1 cut(s) 4687
SchI GAGTC 8 cut(s) 290, 460, 601, 1096, 2266, 3569, 3730, 4247
ScrFI CCNGG 7 cut(s) 864, 1371, 2280, 2342, 2915, 3243, 3810
SduI GDGCHC 3 cut(s) 368, 3724, 4031
SfaNI GCATC 8 cut(s) 527, 1648, 2572, 2868, 3376, 3884, 4199, 4735
SfcI CTRYAG 2 cut(s) 657, 2027
SfoI GGCGCC 2 cut(s) 2888, 3220
SfuI TTCGAA 1 cut(s) 3517
SinI GGWCC 8 cut(s) 1349, 1361, 2501, 3031, 3075, 3245, 4279, 4383
SmiMI CAYNNNNRTG 3 cut(s) 1064, 2972, 4369
SmlI CTYRAG 3 cut(s) 1356, 1583, 4296
SmoI CTYRAG 3 cut(s) 1356, 1583, 4296
SnaBI TACGTA 1 cut(s) 1778
SphI GCATGC 1 cut(s) 1283
SsiI CCGC 9 cut(s) 770, 1162, 1512, 2056, 2151, 3015, 3401, 3569, 4532
SspDI GGCGCC 2 cut(s) 2886, 3218
SspI AATATT 3 cut(s) 2170, 2962, 4327
StyD4I CCNGG 7 cut(s) 862, 1369, 2278, 2340, 2913, 3241, 3808
StyI CCWWGG 2 cut(s) 3277, 4023
TaaI ACNGT 6 cut(s) 480, 1972, 2031, 2539, 2758, 3858
TaiI ACGT 7 cut(s) 709, 1321, 1780, 2407, 2923, 4198, 4714
TatI WGTACW 3 cut(s) 1189, 1289, 3529
TauI GCSGC 1 cut(s) 1515
TspGWI ACGGA 3 cut(s) 2216, 4436, 4449
Tth111I GACNNNGTC 2 cut(s) 280, 610
Van91I CCANNNNNTGG 2 cut(s) 1208, 4144
Vha464I CTTAAG 1 cut(s) 1583
VneI GTGCAC 1 cut(s) 364
VpaK11BI GGWCC 8 cut(s) 1349, 1361, 2501, 3031, 3075, 3245, 4279, 4383
XagI CCTNNNNNAGG 1 cut(s) 1356
XceI RCATGY 5 cut(s) 1283, 1868, 3051, 4101, 4134
XcmI CCANNNNNNNNNTGG 1 cut(s) 1305
XmiI GTMKAC 3 cut(s) 703, 3422, 4708
XmnI GAANNNNTTC 1 cut(s) 574
Zsp2I ATGCAT 1 cut(s) 520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.