MD13G1030100.v1.1

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009426
Physical Location & Seq
Reverse (-)
2168135 .. 2177158
9024 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1030100.v1.1.491

Sequence Viewer

Length: 4809 bp
ATGGACCAAAACCAGAGACCATCCTCCTCGGCGTCAGCTGCCTCGCGAGCTTACCAGTTTCACCCCGCACGCGCAGCAATTGTCAATCTCTTCGACCTCTACTTAGGAAGAAGTAGCCGCCAAAAATCCGATGATTCAATTCGAGAACCTCCGAACAAGTCACAGAAGCGTGTACTTGCTCTTAATCGAGAGCTTCCACCTCGAAACGAGCAGTTCCTACTAGATTTTGAACAACTGCAGAGCCAGCTTCCTGACCAAGATCAACTGCGTGTCGTGACAGAGTCTGTACTTATATCTTTAGTCGTGCAATGCAGCAATCATGCGCCGCAGGCGGAGTTTCTTCTCTTTGCTCTAAGGAGCTTGTGTACTATAGGTCACATTAACTGGGATACTTTTTTGCCATCCCTTCTTTCTTCAGTCTCTGCTGCAGAAATGTCAGTTGGTCAAGGTAGTCAAGCAATGCGTACTGTTTCATCGCAGTCCGGTATGCTTCCATCTTCAAATGCAATCCCCAACTCGTTTAATTTTCAATCTTCAAGTCCTGCCTCTCCGTTACCTTCAGTGCATGGCATTGGTTCCCCCAGCCAGTCTGCTATTGAACCATCATCTATGTCACCAGTTAAATCATCTGACATAGCCTCCAATGGACAGCAGGCTATGGCAAGGTTCAACTCATCTATAAGAGATAATGCCATAAGCAGTCTTCGTCAGCTGTGCTGCAAGATCATTTTAACTGGACTCAAATTTAATTTAAAACCAGTGACTCATGCTAACATCTTTTCCCATATGCTTAATTGGCTGGTTAATTGGGACCAAAAACAACTTGGGGTTGATGAATCTGATAGTGTAAGATCCTGGAGACCTGGAAAGGCGCTAATTGAATGGCTACATAGCTGTTTGGATGTGATTTGGCTTTTGGTTGACGAGGAAAAATGCCGAGTGCCCTTCTATGAATTACTGCGAAGTGGGTTGCAATTTATGGAGAATATACCTGATGATGAAGCCTTATTTACTCTAATCTTGGAGATCCATAGGAGGCGAGATATGATGGCCATGCACATGAAAATGTTAGATCAACACCTACATTGCCCTACATTTGGGACTCATCGGATTTTCACGCAGACAACTCCTAGTGTTTCAGGAGAAGCAGTAGCAACCTTGCGGTATTCACCAATCACATATCCAAGTGTTCTTGGAGAACCATTGCATGGAGAGGACCTTGCAACTTCAATTCCAAAAGGAAGTTTGGATTGGGAGAGAGCTTTGCGTTGCATAAGGCATGCTCTTTGTACTACTCCATCACCCGACTGGTGGAAACGGGTGCTGCTTGTAGCCCCCTGCTACAGGTCTCCTTCTCAAGGACCTACTCCTGGTGCTGTTTTCATCTCTGAAATGATTTGCGAGGCAACAATTGATAGAATTGTTGAGCTATTGAAATTGACCAATTCAGATATTAATTGCTGGCAACAGTGGCTTGTCTTTTCGGATATATTCTTTTTTCTTATTAAAAGTGGCTGTATTGATTTTGTTGATTTTGTGGACAAGCTAGTTTCACGCCTTAACGAGGGTGATCAACAAATTCTTAGGACAAATCATGTCACTTGGCTTCTTGCTCAAATTATTCGGGTTGAACATGTGATGAATGCTCTAAATCAAGATGCTAGAAAGGTGGAGACAACAAGAAAGATCCTATCGTTTCATAAAGAAGATAGGAGCTCAGATCCAAATAGTCCTCAAAGTATACTGCTGGACTTCATCAGCAGTTGCCAGAATTTACGTATTTGGTCATTGAACACGACAACTAGAGAATATCTGAATAATGAGCAGCTTCAGAAAGGAAAGCAAATAGATGAATGGTGGAGGACAGCGAGCAAAGGGGACCGAATGATGGATTATATGAATATGGATGAAAGGTCAATTGGGATGTTTTGGGTTGTGTCTTACACCATGGCGCAGCCAGCTTGTGAAACTGTGATCAATTGGTTATCCTCTGCTGGGGTTATAGAGTCGTTACCAGGAACAAACTTACAGTCCAATGAGAGGCTAATGGTTATGCGGGAAGTATGTCCATTGCCGATGTCCTTGTTATCTGGCTTTTCAATGAACCTATGTTTAAAGCTGGCGTATCAAATGGAAGAATCTTTATTTTCTGGGCAGGTTGTACCTAGTATTGCAATGGCTGAAACATATTGTAGGCTGCTACTCATTGCACCTTACTCATTATTTCGTTCTCATTTCAATCATTTGGCGCAAAGGAATCCCTCGGTATTGAGCAAGCCAGGCGTAACTCTTTTAGTGCTTGAAATATTGAATTACCGTTTGCTTCCACTTTACAGGTACCAAGGAAAAAGCAAAGCCTTGATGTATGATGTTACGAAAATAGTTTCTGCTCTAAAATCAAAACGTGGAGATCACCGTGTATTTAGGTTGGCTGAGAACTTGTGTATGAATCTTATTCTGTCTCTGAGAGACTTTTTCTTGGTGAAAAGAGAAGGGAAGGGTCCAACTGAATTCACAGAAACTTTGAATCGAGTAACAGTTGTCACTCTTGCGATCATCATTAAGACACGAGGAATTGCTGATGCTGATCATCTGCTTTATCTTCAAACCATGTTAGAGCAGATATTAGCCAATAGTAATCACACATGGTCAGAGAAAACACTACGCTATTTCCCTTCTCTTCTACGTGATGTTTTGATCCAACGGATTGATAAAAGGGGCCTGGCAATTCAAGAATGGCAGCAGGCCGAAACCACTGTGATTAACCAATGCACCCAGCTTTTGTCCCCTTCCGCTGATCCTACATATGTAATGACCTATATCAGTCACAGTTTTCCTCAACACCGACAATATCTATGTGCTGGTGCATGGATCCTGATGCAAGGGCACCCAGAAAACATTAACAGCGTAAACTTAGCACGTGTCTTGAGAGAGTTTTCTCCTGAGGAAGTAACATCTAATATTTATACAATGGTGGATGTTCTGCTTCATCATATTCAGTTGGAACTTCAGCATGGTCATTCTTTGCAGGATCTTTTATTGAAAGCTTGTGCAAACCTAGCTTTTTATATTTGGACCCACGAGTTGCTCCCTTTGGATATTTTGCTCCTGGCACTTATTGATCGTGACGATGACCCCCATGCTTTGCATATCGTGATTAGTCTACTTGATCGGCAAGAACTTCAACAGAGGGTGAAACTATATTGTGTGAATCGAGGTCCCCCTGAACACTGGCTTTTCTCTGGACCCTTTAAGCGAGTTGAATTGCAAAAGGCCCTTGGGAATCATCTATCCTGGAAGGACAGGTATCCTACTTTCTTCGATGACATTGCAGGGCGTTTGCTCCCAGTCATTCCGTTGATAATTTATAGACTAATTGAAAATGATGCCATAGATTCAGCTGAAGGAATTCTGGTCATGTATTCTACATTTCTTAAATACCACCCTTTAAGATTTACATTTGTTCGTGACATACTTGCATATTTTTATGGCCATCTTCCCGGAAAGCTCATTGTCCGAATATTGAATGGACTCGAAGTCAACAAGATCCCATTTTCTGAGTCATTCCCAGTGCATATGAATTTATCAAATTCCACCATGTGCCCACCTGCCGATTACTTTGCAACTCTCTTACTGGGACTAGTGAATAATGTCATTCCTCCATTGCATAACAACTCAAAATCTGGAGCGAATGATGCTTTAAATAATTCAATGCGTGCCCCACCTAACAAGACTCCTCCAACGTCGCAGTCTGGGCAAGCAAATGTTTCTGATGGCCAAAAAGCATTCTTTCAAATTCAGGATCCAGGCACATATACTCAGCTGGTTCTTGAAACAGCAGTCATTGAACTGCTCTCTCTTCCTGTATCTTCATCCCAGATTGTATCATCACTGGTTCAGATTGTCATCAATATACAACCAACATTAATTCAATCCAGCAATGGCCTGCATGGAGCTCCAAATGGTGTTGGGCAAGGCTCTGTTTTACCAACATCCCCTTCGGGTGGAAGCACAGATTCCTTGGGCACGACGAGCAGGTCAGCTGCTTCAGGGTCAGGAATAAATGCATCTAACTTTGTTTCTCGAAGTGGTTATACATGCCAACAGTTGTCTTGCTTATTGATCCAAGCATGTGGTCTTCTACTGGCTCAACTGCCTCCTGATTTTCATGTGCAACTCTATCTAGAGGCATCACGTATAATAAAAGAAACGTGGTGGCTCACTGATGGCAAAAGGTCAATGGGAGAATTAGACTCTGCTGTCGGTTATGCTTTGTTGGATCCAACATGGGCTGCTCAAGACAATACCTCGACCGCCATTGGTAATATTGTTGCCCTGCTCCATTCGTTTTTCAGTAACCTCCCACAAGAGTGGCTAGAAGGGACCCATCTTATCATCAAACATCTCAGGCCGGTGACTTCAGTTGCAATGTTGAGAATAGCATTCCGAATAATGGGTCCCTTGCTCCCTAAACTAGCCAATGCGCACGCCCTCTTCAGTAAGGCTCTGTCATTGATTTTAAGTATGATGGTAGATGTCTTCGGGAAGAATGTACAGCCTCCGATCCCAGTCGAGCCATTAGAAATAGCAGATATAATTGACTTCTTCCATCACGTCGTCCACTATGAAGGGCAGGGAGGTCCTGTGCAGGCTAACAGCAAGCCCAGACCAGAGGTTTTAGCTCTCTGTGGAAGAGCTGCGGAAAGTCTGCGTCCGGACATACAACACCTTCTTTCCCACTTAAAACCCGACACGAATTCTTCCATTTACGCTGCTACGCATCCCAAGTTGAACCAGAATTCGTCGTAA

Protein Analysis

1603

Amino Acids

179.61

Weight (kDa)

6.7

Isoelectric Point (pI)

47.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med23 PF11573 489 - 1215 8.9e-30 Mediator complex subunit 23
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3616
AasI GACNNNNNNGTC 2 cut(s) 4036, 4259
Acc16I TGCGCA 1 cut(s) 4485
Acc36I ACCTGC 3 cut(s) 2146, 3616, 4026
Acc65I GGTACC 1 cut(s) 2337
AccB1I GGYRCC 2 cut(s) 2337, 2886
AccB7I CCANNNNNTGG 1 cut(s) 1208
AccI GTMKAC 2 cut(s) 1741, 3163
AccII CGCG 2 cut(s) 46, 72
AccIII TCCGGA 1 cut(s) 4714
AciI CCGC 9 cut(s) 66, 118, 326, 332, 1162, 2056, 2793, 4314, 4700
AcoI YGGCCR 3 cut(s) 1050, 3490, 3775
AcuI CTGAAG 8 cut(s) 399, 543, 1814, 2993, 3423, 4032, 4404, 4480
AcvI CACGTG 1 cut(s) 2921
AcyI GRCGYC 1 cut(s) 32
AdeI CACNNNGTG 1 cut(s) 3600
AfaI GTAC 8 cut(s) 174, 288, 367, 466, 1291, 2163, 2339, 4554
AflIII ACRYGT 2 cut(s) 1633, 2920
AhdI GACNNNNNGTC 1 cut(s) 3536
AhlI ACTAGT 1 cut(s) 3640
AjiI CACGTC 1 cut(s) 4615
AjnI CCWGG 9 cut(s) 854, 862, 1369, 2014, 2278, 2721, 3108, 3293, 3805
AjuI GAANNNNNNNTTGG 8 cut(s) 423, 455, 1234, 1266, 1902, 1934, 3982, 4014
AleI CACNNNNGTG 1 cut(s) 4369
Alw21I GWGCWC 2 cut(s) 1718, 3958
Alw26I GTCTC 7 cut(s) 10, 424, 853, 1353, 1667, 2463, 2466
AlwNI CAGNNNCTG 2 cut(s) 284, 422
Aor13HI TCCGGA 1 cut(s) 4714
AoxI GGCC 8 cut(s) 1050, 2719, 2745, 3273, 3490, 3775, 3943, 4409
ArsI GACNNNNNNTTYG 4 cut(s) 2015, 2047, 4441, 4473
AseI ATTAAT 2 cut(s) 1455, 3926
Asp700I GAANNNNTTC 1 cut(s) 3408
Asp718I GGTACC 1 cut(s) 2337
AspLEI GCGC 6 cut(s) 74, 325, 874, 1954, 2251, 4486
AsuC2I CCSGG 1 cut(s) 3501
AsuHPI GGTGA 9 cut(s) 53, 606, 1161, 1293, 1580, 2405, 2494, 3205, 4426
AxyI CCTNAGG 1 cut(s) 2943
BaeGI GKGCMC 5 cut(s) 945, 2889, 3605, 3721, 4028
BalI TGGCCA 3 cut(s) 1052, 3492, 3777
BamHI GGATCC 3 cut(s) 2871, 3802, 4279
BanI GGYRCC 2 cut(s) 2337, 2886
BanII GRGCYC 2 cut(s) 1718, 3958
BarI GAAGNNNNNNTAC 2 cut(s) 4078, 4110
BauI CACGAG 2 cut(s) 2568, 3080
BbrPI CACGTG 1 cut(s) 2921
BbsI GAAGAC 3 cut(s) 695, 4130, 4531
Bbv12I GWGCWC 2 cut(s) 1718, 3958
BcgI CGANNNNNNTGC 4 cut(s) 3311, 3345, 3602, 3636
BciT130I CCWGG 9 cut(s) 856, 864, 1371, 2016, 2280, 2723, 3110, 3295, 3807
BciVI GTATCC 2 cut(s) 382, 3318
BclI TGATCA 3 cut(s) 1570, 1974, 2587
BcnI CCSGG 1 cut(s) 3501
BcoDI GTCTC 7 cut(s) 10, 424, 853, 1353, 1667, 2463, 2466
BcuI ACTAGT 1 cut(s) 3640
BfmI CTRYAG 4 cut(s) 236, 369, 426, 1342
BfoI RGCGCY 1 cut(s) 875
BfuAI ACCTGC 3 cut(s) 2146, 3616, 4026
BfuI GTATCC 2 cut(s) 382, 3318
BmeRI GACNNNNNGTC 1 cut(s) 3536
BmgBI CACGTC 1 cut(s) 4615
BmrI ACTGGG 5 cut(s) 394, 3341, 3563, 3644, 4562
BmsI GCATC 8 cut(s) 1648, 2572, 2868, 3376, 3685, 4076, 4199, 4789
BmuI ACTGGG 5 cut(s) 394, 3341, 3563, 3644, 4562
BpiI GAAGAC 3 cut(s) 695, 4130, 4531
BpmI CTGGAG 2 cut(s) 877, 3705
BpuEI CTTGAG 3 cut(s) 1341, 2947, 4281
BpuMI CCSGG 1 cut(s) 3501
BsaAI YACGTR 4 cut(s) 1778, 2687, 2921, 4196
BsaBI GATNNNNATC 2 cut(s) 2586, 3905
BsaHI GRCGYC 1 cut(s) 32
BsaI GGTCTC 3 cut(s) 10, 853, 1353
BsaJI CCNNGG 6 cut(s) 27, 1947, 2262, 2341, 3277, 4020
BsaWI WCCGGW 2 cut(s) 482, 4714
BsaXI ACNNNNNCTCC 8 cut(s) 349, 379, 623, 653, 1134, 1164, 4629, 4659
Bse118I RCCGGY 1 cut(s) 4411
Bse21I CCTNAGG 1 cut(s) 2943
Bse8I GATNNNNATC 2 cut(s) 2586, 3905
BseAI TCCGGA 1 cut(s) 4714
BseBI CCWGG 9 cut(s) 856, 864, 1371, 2016, 2280, 2723, 3110, 3295, 3807
BseDI CCNNGG 6 cut(s) 27, 1947, 2262, 2341, 3277, 4020
BseGI GGATG 9 cut(s) 20, 401, 907, 1912, 1929, 2983, 3872, 3992, 4780
BseJI GATNNNNATC 2 cut(s) 2586, 3905
BseMII CTCAG 7 cut(s) 1731, 2424, 2456, 2934, 3549, 3833, 4420
BseRI GAGGAG 2 cut(s) 16, 3726
BseSI GKGCMC 5 cut(s) 945, 2889, 3605, 3721, 4028
BseYI CCCAGC 3 cut(s) 581, 1994, 2775
BsgI GTGCAG 1 cut(s) 4667
Bsh1236I CGCG 2 cut(s) 46, 72
Bsh1285I CGRYCG 1 cut(s) 4314
BshFI GGCC 8 cut(s) 1052, 2721, 2747, 3275, 3492, 3777, 3945, 4411
BshNI GGYRCC 2 cut(s) 2337, 2886
BsiEI CGRYCG 1 cut(s) 4314
BsiHKAI GWGCWC 2 cut(s) 1718, 3958
BsiSI CCGG 4 cut(s) 483, 3501, 4412, 4715
BslFI GGGAC 8 cut(s) 824, 1114, 1892, 2770, 3204, 3651, 4396, 4443
BsmAI GTCTC 7 cut(s) 10, 424, 853, 1353, 1667, 2463, 2466
BsmFI GGGAC 8 cut(s) 824, 1114, 1892, 2770, 3204, 3651, 4396, 4443
BsmI GAATGC 3 cut(s) 1648, 3785, 4442
BsnI GGCC 8 cut(s) 1052, 2721, 2747, 3275, 3492, 3777, 3945, 4411
Bso31I GGTCTC 3 cut(s) 10, 853, 1353
Bsp1286I GDGCHC 7 cut(s) 945, 1718, 2889, 3605, 3721, 3958, 4028
Bsp13I TCCGGA 1 cut(s) 4714
Bsp1407I TGTACA 1 cut(s) 4552
Bsp19I CCATGG 1 cut(s) 1947
Bsp68I TCGCGA 1 cut(s) 46
BspACI CCGC 9 cut(s) 66, 118, 326, 332, 1162, 2056, 2793, 4314, 4700
BspANI GGCC 8 cut(s) 1052, 2721, 2747, 3275, 3492, 3777, 3945, 4411
BspCNI CTCAG 7 cut(s) 1730, 2425, 2457, 2935, 3550, 3832, 4419
BspEI TCCGGA 1 cut(s) 4714
BspFNI CGCG 2 cut(s) 46, 72
BspMAI CTGCAG 2 cut(s) 240, 430
BspMI ACCTGC 3 cut(s) 2146, 3616, 4026
BspQI GCTCTTC 1 cut(s) 4687
BspT107I GGYRCC 2 cut(s) 2337, 2886
BspTNI GGTCTC 3 cut(s) 10, 853, 1353
BsrFI RCCGGY 1 cut(s) 4411
BsrGI TGTACA 1 cut(s) 4552
BssAI RCCGGY 1 cut(s) 4411
BssECI CCNNGG 6 cut(s) 27, 1947, 2262, 2341, 3277, 4020
BssNAI GTATAC 1 cut(s) 1742
BssNI GRCGYC 1 cut(s) 32
BssSI CACGAG 2 cut(s) 2568, 3080
BssT1I CCWWGG 4 cut(s) 1947, 2341, 3277, 4020
Bst1107I GTATAC 1 cut(s) 1742
Bst2BI CACGAG 2 cut(s) 2568, 3080
Bst2UI CCWGG 9 cut(s) 856, 864, 1371, 2016, 2280, 2723, 3110, 3295, 3807
Bst6I CTCTTC 5 cut(s) 95, 2685, 3864, 4499, 4687
BstACI GRCGYC 1 cut(s) 32
BstAUI TGTACA 1 cut(s) 4552
BstBAI YACGTR 4 cut(s) 1778, 2687, 2921, 4196
BstDSI CCRYGG 1 cut(s) 1947
BstENI CCTNNNNNAGG 3 cut(s) 1342, 1356, 1562
BstF5I GGATG 9 cut(s) 20, 401, 907, 1912, 1929, 2983, 3872, 3992, 4780
BstFNI CGCG 2 cut(s) 46, 72
BstH2I RGCGCY 1 cut(s) 875
BstHHI GCGC 6 cut(s) 74, 325, 874, 1954, 2251, 4486
BstMAI GTCTC 7 cut(s) 10, 424, 853, 1353, 1667, 2463, 2466
BstMCI CGRYCG 1 cut(s) 4314
BstNI CCWGG 9 cut(s) 856, 864, 1371, 2016, 2280, 2723, 3110, 3295, 3807
BstNSI RCATGY 4 cut(s) 1283, 1637, 4101, 4134
BstSFI CTRYAG 4 cut(s) 236, 369, 426, 1342
BstSLI GKGCMC 5 cut(s) 945, 2889, 3605, 3721, 4028
BstSNI TACGTA 1 cut(s) 1778
BstUI CGCG 2 cut(s) 46, 72
BstV2I GAAGAC 3 cut(s) 695, 4130, 4531
BstX2I RGATCY 9 cut(s) 851, 1026, 1686, 1720, 2871, 3031, 3546, 3802, 4279
BstXI CCANNNNNNTGG 2 cut(s) 4133, 4371
BstYI RGATCY 9 cut(s) 851, 1026, 1686, 1720, 2871, 3031, 3546, 3802, 4279
BstZ17I GTATAC 1 cut(s) 1742
Bsu36I CCTNAGG 1 cut(s) 2943
BsuI GTATCC 2 cut(s) 382, 3318
BsuRI GGCC 8 cut(s) 1052, 2721, 2747, 3275, 3492, 3777, 3945, 4411
BtgI CCRYGG 1 cut(s) 1947
BtgZI GCGATG 1 cut(s) 459
BtrI CACGTC 1 cut(s) 4615
BtsCI GGATG 9 cut(s) 20, 401, 907, 1912, 1929, 2983, 3872, 3992, 4780
BtsIMutI CAGTG 8 cut(s) 567, 765, 1475, 2754, 3229, 3576, 3890, 4221
BtuMI TCGCGA 1 cut(s) 46
BveI ACCTGC 3 cut(s) 2146, 3616, 4026
CaiI CAGNNNCTG 2 cut(s) 284, 422
CfoI GCGC 6 cut(s) 74, 325, 874, 1954, 2251, 4486
Cfr10I RCCGGY 1 cut(s) 4411
CseI GACGC 2 cut(s) 21, 4700
Csp6I GTAC 8 cut(s) 173, 287, 366, 465, 1290, 2162, 2338, 4553
CviQI GTAC 8 cut(s) 173, 287, 366, 465, 1290, 2162, 2338, 4553
DraI TTTAAA 3 cut(s) 753, 2115, 3702
DraIII CACNNNGTG 1 cut(s) 3600
DrdI GACNNNNNNGTC 2 cut(s) 4036, 4259
DriI GACNNNNNGTC 1 cut(s) 3536
DseDI GACNNNNNNGTC 2 cut(s) 4036, 4259
EaeI YGGCCR 3 cut(s) 1050, 3490, 3775
Eam1104I CTCTTC 5 cut(s) 95, 2685, 3864, 4499, 4687
Eam1105I GACNNNNNGTC 1 cut(s) 3536
EarI CTCTTC 5 cut(s) 95, 2685, 3864, 4499, 4687
EciI GGCGGA 1 cut(s) 347
Ecl136II GAGCTC 2 cut(s) 1716, 3956
Eco105I TACGTA 1 cut(s) 1778
Eco130I CCWWGG 4 cut(s) 1947, 2341, 3277, 4020
Eco24I GRGCYC 2 cut(s) 1718, 3958
Eco31I GGTCTC 3 cut(s) 10, 853, 1353
Eco53kI GAGCTC 2 cut(s) 1716, 3956
Eco57I CTGAAG 8 cut(s) 399, 543, 1814, 2993, 3423, 4032, 4404, 4480
Eco72I CACGTG 1 cut(s) 2921
Eco81I CCTNAGG 1 cut(s) 2943
EcoICRI GAGCTC 2 cut(s) 1716, 3956
EcoNI CCTNNNNNAGG 3 cut(s) 1342, 1356, 1562
EcoO109I RGGNCCY 8 cut(s) 1216, 1361, 2719, 3218, 3274, 4383, 4457, 4640
EcoRI GAATTC 4 cut(s) 2510, 3408, 4756, 4798
EcoRII CCWGG 9 cut(s) 854, 862, 1369, 2014, 2278, 2721, 3108, 3293, 3805
EcoT14I CCWWGG 4 cut(s) 1947, 2341, 3277, 4020
EcoT22I ATGCAT 1 cut(s) 4069
EcoT38I GRGCYC 2 cut(s) 1718, 3958
ErhI CCWWGG 4 cut(s) 1947, 2341, 3277, 4020
FaqI GGGAC 8 cut(s) 824, 1114, 1892, 2770, 3204, 3651, 4396, 4443
FauI CCCGC 2 cut(s) 73, 2049
FauNDI CATATG 3 cut(s) 786, 2806, 3576
FbaI TGATCA 3 cut(s) 1570, 1974, 2587
FblI GTMKAC 2 cut(s) 1741, 3163
FokI GGATG 9 cut(s) 7, 388, 914, 1919, 1936, 2990, 3859, 3979, 4767
FriOI GRGCYC 2 cut(s) 1718, 3958
FspAI RTGCGCAY 1 cut(s) 4485
FspI TGCGCA 1 cut(s) 4485
GlaI GCGC 6 cut(s) 73, 324, 873, 1953, 2250, 4485
GsaI CCCAGC 3 cut(s) 585, 1998, 2779
GsuI CTGGAG 2 cut(s) 877, 3705
HaeII RGCGCY 1 cut(s) 875
HaeIII GGCC 8 cut(s) 1052, 2721, 2747, 3275, 3492, 3777, 3945, 4411
HapII CCGG 4 cut(s) 483, 3501, 4412, 4715
HgaI GACGC 2 cut(s) 21, 4700
HhaI GCGC 6 cut(s) 74, 325, 874, 1954, 2251, 4486
Hin1I GRCGYC 1 cut(s) 32
Hin6I GCGC 6 cut(s) 72, 323, 872, 1952, 2249, 4484
HinP1I GCGC 6 cut(s) 72, 323, 872, 1952, 2249, 4484
HincII GTYRAC 2 cut(s) 922, 3541
HindII GTYRAC 2 cut(s) 922, 3541
HindIII AAGCTT 1 cut(s) 3045
HpaII CCGG 4 cut(s) 483, 3501, 4412, 4715
HphI GGTGA 9 cut(s) 53, 606, 1161, 1293, 1580, 2405, 2494, 3205, 4426
Hpy166II GTNNAC 9 cut(s) 173, 366, 922, 1540, 1742, 2911, 3164, 3541, 4621
Hpy8I GTNNAC 9 cut(s) 173, 366, 922, 1540, 1742, 2911, 3164, 3541, 4621
Hpy99I CGWCG 4 cut(s) 3748, 4033, 4619, 4807
HpyCH4IV ACGT 8 cut(s) 1777, 2404, 2686, 2920, 3743, 4195, 4211, 4614
HpySE526I ACGT 8 cut(s) 1777, 2404, 2686, 2920, 3743, 4195, 4211, 4614
Hsp92I GRCGYC 1 cut(s) 32
HspAI GCGC 6 cut(s) 72, 323, 872, 1952, 2249, 4484
KflI GGGWCCC 2 cut(s) 4383, 4457
Kpn2I TCCGGA 1 cut(s) 4714
KpnI GGTACC 1 cut(s) 2341
Ksp22I TGATCA 3 cut(s) 1570, 1974, 2587
LguI GCTCTTC 1 cut(s) 4687
LweI GCATC 8 cut(s) 1648, 2572, 2868, 3376, 3685, 4076, 4199, 4789
MaeII ACGT 8 cut(s) 1777, 2404, 2686, 2920, 3743, 4195, 4211, 4614
MfeI CAATTG 4 cut(s) 78, 1410, 1917, 1978
MflI RGATCY 9 cut(s) 851, 1026, 1686, 1720, 2871, 3031, 3546, 3802, 4279
MhlI GDGCHC 7 cut(s) 945, 1718, 2889, 3605, 3721, 3958, 4028
MlsI TGGCCA 3 cut(s) 1052, 3492, 3777
MluNI TGGCCA 3 cut(s) 1052, 3492, 3777
MlyI GAGTC 9 cut(s) 290, 732, 757, 1096, 2015, 3525, 3569, 3727, 4247
MmeI TCCRAC 6 cut(s) 2528, 2725, 2982, 3764, 4257, 4307
Mox20I TGGCCA 3 cut(s) 1052, 3492, 3777
Mph1103I ATGCAT 1 cut(s) 4069
MroI TCCGGA 1 cut(s) 4714
MroXI GAANNNNTTC 1 cut(s) 3408
MscI TGGCCA 3 cut(s) 1052, 3492, 3777
MslI CAYNNNNRTG 3 cut(s) 1058, 1064, 4369
Msp20I TGGCCA 3 cut(s) 1052, 3492, 3777
MspA1I CMGCKG 6 cut(s) 38, 712, 2795, 3401, 3823, 4043
MspI CCGG 4 cut(s) 483, 3501, 4412, 4715
MunI CAATTG 4 cut(s) 78, 1410, 1917, 1978
Mva1269I GAATGC 3 cut(s) 1648, 3785, 4442
MvaI CCWGG 9 cut(s) 856, 864, 1371, 2016, 2280, 2723, 3110, 3295, 3807
MvnI CGCG 2 cut(s) 46, 72
NciI CCSGG 1 cut(s) 3501
NcoI CCATGG 1 cut(s) 1947
NdeI CATATG 3 cut(s) 786, 2806, 3576
NmeAIII GCCGAG 2 cut(s) 8, 962
NruI TCGCGA 1 cut(s) 46
NsbI TGCGCA 1 cut(s) 4485
NsiI ATGCAT 1 cut(s) 4069
NspI RCATGY 4 cut(s) 1283, 1637, 4101, 4134
OliI CACNNNNGTG 1 cut(s) 4369
PaeI GCATGC 1 cut(s) 1283
PaqCI CACCTGC 1 cut(s) 3616
PciI ACATGT 1 cut(s) 1633
PciSI GCTCTTC 1 cut(s) 4687
PctI GAATGC 3 cut(s) 1648, 3785, 4442
PdmI GAANNNNTTC 1 cut(s) 3408
PflFI GACNNNGTC 1 cut(s) 280
PflMI CCANNNNNTGG 1 cut(s) 1208
PfoI TCCNGGA 3 cut(s) 854, 3293, 3499
PleI GAGTC 9 cut(s) 289, 732, 757, 1096, 2014, 3525, 3568, 3727, 4247
PmaCI CACGTG 1 cut(s) 2921
PmlI CACGTG 1 cut(s) 2921
PpsI GAGTC 9 cut(s) 289, 732, 757, 1096, 2014, 3525, 3568, 3727, 4247
Ppu21I YACGTR 4 cut(s) 1778, 2687, 2921, 4196
PpuMI RGGWCCY 6 cut(s) 1216, 1361, 3218, 4383, 4457, 4640
PscI ACATGT 1 cut(s) 1633
PshBI ATTAAT 2 cut(s) 1455, 3926
Psp124BI GAGCTC 2 cut(s) 1718, 3958
Psp5II RGGWCCY 6 cut(s) 1216, 1361, 3218, 4383, 4457, 4640
Psp6I CCWGG 9 cut(s) 854, 862, 1369, 2014, 2278, 2721, 3108, 3293, 3805
PspCI CACGTG 1 cut(s) 2921
PspFI CCCAGC 3 cut(s) 581, 1994, 2775
PspGI CCWGG 9 cut(s) 854, 862, 1369, 2014, 2278, 2721, 3108, 3293, 3805
PspPPI RGGWCCY 6 cut(s) 1216, 1361, 3218, 4383, 4457, 4640
PstI CTGCAG 2 cut(s) 240, 430
PstNI CAGNNNCTG 2 cut(s) 284, 422
PsuI RGATCY 9 cut(s) 851, 1026, 1686, 1720, 2871, 3031, 3546, 3802, 4279
PsyI GACNNNGTC 1 cut(s) 280
PvuII CAGCTG 5 cut(s) 38, 712, 3401, 3823, 4043
RruI TCGCGA 1 cut(s) 46
RsaI GTAC 8 cut(s) 174, 288, 367, 466, 1291, 2163, 2339, 4554
RsaNI GTAC 8 cut(s) 173, 287, 366, 465, 1290, 2162, 2338, 4553
RseI CAYNNNNRTG 3 cut(s) 1058, 1064, 4369
SacI GAGCTC 2 cut(s) 1718, 3958
SapI GCTCTTC 1 cut(s) 4687
SchI GAGTC 9 cut(s) 290, 732, 757, 1096, 2015, 3525, 3569, 3727, 4247
SduI GDGCHC 7 cut(s) 945, 1718, 2889, 3605, 3721, 3958, 4028
SfaNI GCATC 8 cut(s) 1648, 2572, 2868, 3376, 3685, 4076, 4199, 4789
SfcI CTRYAG 4 cut(s) 236, 369, 426, 1342
SmiMI CAYNNNNRTG 3 cut(s) 1058, 1064, 4369
SmlI CTYRAG 3 cut(s) 1356, 2926, 4296
SmoI CTYRAG 3 cut(s) 1356, 2926, 4296
SnaBI TACGTA 1 cut(s) 1778
SpeI ACTAGT 1 cut(s) 3640
SphI GCATGC 1 cut(s) 1283
SsiI CCGC 9 cut(s) 66, 118, 326, 332, 1162, 2056, 2793, 4314, 4700
SspI AATATT 4 cut(s) 2307, 2962, 3522, 4327
SstI GAGCTC 2 cut(s) 1718, 3958
StyI CCWWGG 4 cut(s) 1947, 2341, 3277, 4020
TaiI ACGT 8 cut(s) 1780, 2407, 2689, 2923, 3746, 4198, 4214, 4617
TaqII GACCGA 1 cut(s) 1896
TatI WGTACW 5 cut(s) 172, 286, 365, 1289, 4552
TauI GCSGC 2 cut(s) 120, 328
TscAI CASTG 8 cut(s) 567, 765, 1475, 2761, 3236, 3576, 3897, 4228
TspGWI ACGGA 3 cut(s) 540, 2719, 3345
TspRI CASTG 8 cut(s) 567, 765, 1475, 2761, 3236, 3576, 3897, 4228
Tth111I GACNNNGTC 1 cut(s) 280
Van91I CCANNNNNTGG 1 cut(s) 1208
VspI ATTAAT 2 cut(s) 1455, 3926
XagI CCTNNNNNAGG 3 cut(s) 1342, 1356, 1562
XbaI TCTAGA 1 cut(s) 4183
XceI RCATGY 4 cut(s) 1283, 1637, 4101, 4134
XcmI CCANNNNNNNNNTGG 2 cut(s) 821, 1305
XmiI GTMKAC 2 cut(s) 1741, 3163
XmnI GAANNNNTTC 1 cut(s) 3408
Zsp2I ATGCAT 1 cut(s) 4069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.