MD16G1020900.v1.1

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009426
Physical Location & Seq
Reverse (-)
1506010 .. 1515181
9172 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1020900.v1.1.491

Sequence Viewer

Length: 4797 bp
ATGGATCAAAACCAGCGACCATCGTCCTCGGCGTCAGCTGCCACGCGAGCTTACCAGTTCCACCCCGCACGTGCCGCAATTGTCAATCTCTTCGATCTCTACTTAGGAAGAAGTAGCCGCCAAAAACCTGATGATTCAGTTCGAGAGCCTCCGAACAAGTCACAGAAGCGTGTACTTGCTCTTAATCGAGAGCTTCCACCTCGAAACGAGCAGTTCCTACTAGATTTTGAACAACTGCAGAGCCAGTTTCCTGACCAAGATCAACTGCGCGTCGTGACAGAATCTGTACTTATATCTCTAGTTGTGCAATGTAGTAATCATGCGCCACGGGCGGAGTTTCTTCTCTTTGCTCTAAGGAGCTTGTGTACTATAGGTCACATTAACTGGGATACTTTTTTGCCGTCCCTTCTTTCTTCGGTTTCCACAGCAGAAATGTCAGTTGGTCAAGGGAGTCAGGCAATGCCTGCTATTTCACCGCAGTCTGGTATGCTTCCATCTTCGAATGCAATCCCCAATTCTTCTAATTTTCAATCTTCAACTCCCGCCTCTCCATTACCTTCAGTGCATGGCATTGGTTCCCCCAGCCAGTCTGCTATCGAACCATCATCTATGTCACCAGTTAAATCATCTGATGTAGCCTCCAATGGACAGCAAGCTACGGCAAGGTTCAATTCATCTATAAGAGATAATGCCATAAGCAGTCTTCGTCAGCTGTGCTGCAAGATCATTTTAACTGGACTTGCATTTAATTTAAAACCAGTTACTCATGCTAACATCTTTTCCCATATGCTTAATTGGCTGGTTAATTGGGACCAAAAACAACTTGGGGTTGATGAACCTGATGGTGTAAGATCCTGGAGACCTGGAAAGGCGCTAATTGAATGGCTACATAGCTGTTTGGATGTGATTTGGCTTTTGGTTGACGAGGATAAATGCCGAGTGCCCTTCTATGAATTACTGCGGAGTGGGTTGCAATTTATGGAGAATATACCTGATGATGAAGCCTTATTTACTCTAATCTTGGAGATCCATAGGAGGCGAGATATGATGGCCATGCACATGAAAATGTTAGATCAACACCTACATTGCCCTACATTTGGGACTCATCGGATTTTCACACAGACAACTCCTAGTGTTTCAGGAGAAGCAGTAGCGAGCTTGAGGTATTCACCAATCACATATCCAAGTGTTCTGGGAGAACCATTGCATGGAGAGGAACTTGCAACTTCAATTCCAAAAGGAAGTTTGGATTGGGAGAGAGCTCTGCGTTGCATAAGGCATGCTCTATGTACTACTCCGTCACCTGACTGGTGGAAACGGGTGCTGCTTGTTGCCCCTTGCTACAGGTCTCCTTCTCAAGGGCCTACTCCTGGTGCTGTTTTCATCTCTGAAATGATTTGCGAGGCAACAATTGATAGAATTGTTGAGCTATTAAAGTTGACCAATTCAGATATTAATTGCTGGCAAGAGTGGCTTGTCTTTTCGGATATATTCTTTTTTCTTATTAAAAGTGGCTGTATTGATTTTGTTGATTTTGTGGACAAGCTAGTTTCACGCCTTACCGAGGGTGACCAACAAATTCTTAGGACAAATCACGTCACTTGGCTTCTTGCTCAAATTATACGGGTTGAACTTGTGATGAATGCTCTAAATCAAGATGCTAGAAAGGTGGAGACAACACGTAAGATCCTATCGTTTCATAAAGAAGATAGGAGCTCAGATCCAAATAGTCCTCAAAGTATCCTGCTGGACTTCATAAGCAGTTGCCAGAATTTACGTATTTGGTCGTTGAACACAACAACTAGAGAATATCTGAATAACGAGCAGCTTCAGAAAGGAAAGCAAATAGATGAATGGTGGAGGACAGCAAGCAAAGGGGATCGAATAATGGATTATATGAATATGGATGAAAGGTCAATTGGGATGTTTTGGGTTGTGTCTTACACCATGGCGCAGCCAGCTTGTGAAACTGTGATAAATTGGTTATCCTCTGCTGGAGTTGCAGAGTCATTACCGGGAACAAACCTACAGTCCAATGAGAGGTTAATGGTTATGCAGGAAGTATCTCCACTGCCGATGTCCTTGTTATCTGGCTTTTCAATGAACCTATGTTTAAAGCTGGCGTATCAAATGGAAGAATCTTTATTTTCTGGGCAGGTTGTACCTAGTATTGCAATGGCTGAAACATATTGTAGGCTGCTACTCATTGCACCTCACTCATTATTTCGTTCTCATTTCAATCATTTGGCGCAAAGGAATCCCTCAGTATTGAGCAAGCCAGGTGTAACTCTTTTAGTGCTTGAAATTTTGAATTACCGTTTGCTTCCACTTTACAGGTACCAAGGAAAAAGCAAAGCCTTGATGTATGATGTTACAAAAATAGTTTCTGCTCTAAAATCAAAACGTGGAGACCATCGTGTATTTAGGTTGGCTGAGAACTTGTGTATGAATCTTATTCTGTCTTTGAGAGACTTTTTCTTTGTGAAAAGGGAAGGGAAGGGTCCAACTGAATTCACAGAAACTTTGAATCGAGTAACTATTGTCACTCTTGCGATCATCATTAAGACACGAGGAATTGCTGATGCTGATCATCTGCTTTATCTTCAAACCATGTTAGAGCAGATATTAGCCACTAGTAATCACACATGGTCAGAGAAAACACTACGCTATTTCCCTTCTCTTCTTCGTGATTTTTTGATTCAACGGATTGATAAAAGGGGCCTGGCAATTCAAGAATGGCAGCAGGCGGAAACCACTGTAATTAACCAATGCACCCAGCTTTTGTCACCTTCCGCTGATCCTACATATGTAATGACCTATATCAGTCATAGTTTTCCTCAACACCGGCAATATCTATGTGCTGGTGCATGGATTCTGATGCAAGGGCACCCAGAAAACATCAACAGCCTAAACTTAGCACGCGTCTTGAGAGAGTTTTCTCCTGAGGAAGTAACATCTAATATCTATACAATGGTGGATGTTCTGCTTCATCATATTCAGTTGGAACTGCAGCACGGCCATTCTTTGCAGGACCTTTTATTGAAAGCATGTGCAAACCTAGCATTTTATATTTGGACCCATGAGTTGATCCCTTTGGATATTTTGCTCCTGGCACTGATTGATCGTGACGATGACCCCCATGCTCTGCATATCGTGATGAGTCTACTTGATAGGCAAGAACTTCAACAAAGGGTGAAACAATATTGTTCGAATCGAGGTCCCCCTGAACACTGGCTTGTCTCTACATCCTTTAAGCGAGTTGAATTGCAGAAGGCCCTCGGGAATCATCTATCTTGGAAGGACAGGTATCCCACCTTCTTCGATGATATTGCAGGGCGTTTGATCCCAGTCATTCCATTGATAATTTATAGACTAATTGAAAATGATGCCATAGATTCAGCTGACAGAATTCTGGTCATGTATTCTCCATTTCTTAAATACCACCCTTTAAGATTTACTTTTGTTCGTGACATACTTGCATATTTTTATGGCCATCTTCCCGGAAAGCTTATTGTCCGAATATTGAATGTACTCGAAGTCAACAAGATCCCATTTTCTGAGTCATTCCCGGTGCATATGAATAATGCCATGTGCCCACCTGCCGATTACTTTGCAACTCTCTTACTGGGACTAGTGAATAATGTCATTCCCCCATTGCATAACAACTCAAAATCTGGAACGAGTGATGCTTTAAATAATTCAATGCGTGCCCCACCTAACAAGACTCCACCACCTTCTCAGTCTGGGCAAGCAAATGTTTCCGATGGCCAAAAAGCATTCTACCAAATTCAGGATCCAGGCACATATACTCAGCTGGTTCTTGAAACAGCAGTAATTGAATTGCTCTCTCTTCCTGTATCTGCATCCCAGATTGTATCATCACTTGTTCAGATTGTCGTCAATATACAACCAACATTAATTCAATCCAGCAATGGCCTGCACGGAGCTCCAAGTGGTGTTGGGCAAGGCTCCGTTTTACCAACATCCCCTTCGGGCGGAAGCACAGATTCCTTGGGCACGAGCAGGTCACCTGCTTCAGTATCAGGAATAAATGCATCTAACTTTGTTTCTCGAAGTGGTTATACATGCCAACAATTGTCTTGCTTATTGATCCAAGCATGTGGTCTACTACTGGCTCAGCTGCCTCCTGATTTTCATGTGCAACTCTATCTGGAGGCGTCGCGTATAATAAAAGAAACGTGGTGGCTCACTGATGGCAAACGGTCAATGGGGGAACTGGACTCTGCTGTCGGTTATGCTTTGTTGGATCCAACATGGGCTGCTCAAGACAATACCTCAACCGCCATTGGTAATATTGTTGCGCTGCTTCATTCGTTTTTCAGTAACCTCCCACAGGAATGGCTAGAAGGGACCCATCTTATCATCAAACATCTCAGGCCAGTGACTTCAGTTGCAATGTTGAGAATAGCATTCCGCATCATGGGTCCGTTGCTCCCTAAACTAGCCAATGCGCACGCCCTCTTCAGTAAGACCCTGTCATTGATTTTAAGTATGATGGTGGATGTCTTTGGTAAGAACGTACAGCCACCGACTCCTGTTGAGCCATTAGAAATAGCAGATCTTATTGACTTCTTCCATCATGTGGTCCATTACGAAGGGCAGGGAGGTCCGGTGCAAGCTAACAGCAAGCCCAGACCAGAGGTTTTAGCTCTCTGTGGAAGAGCTGCAGAAAGTCTGCGTCCGGACATACAACACCTTCTTTCCCACTTAAAACCCGACACGAATTCTTCCATCTATGCTGCTACGCATCCCAAGTTGAACCAGAATTCATCGTAA

Protein Analysis

1599

Amino Acids

179.15

Weight (kDa)

6.6

Isoelectric Point (pI)

48.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med23 PF11573 488 - 1165 4.5e-29 Mediator complex subunit 23
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 3607, 4039
AasI GACNNNNNNGTC 1 cut(s) 4247
Acc16I TGCGCA 1 cut(s) 4473
Acc36I ACCTGC 4 cut(s) 2146, 3607, 4014, 4039
Acc65I GGTACC 1 cut(s) 2337
AccB1I GGYRCC 2 cut(s) 2337, 2886
AccB7I CCANNNNNTGG 2 cut(s) 1208, 4603
AccI GTMKAC 2 cut(s) 3163, 4126
AccII CGCG 4 cut(s) 46, 270, 2922, 4183
AccIII TCCGGA 1 cut(s) 4702
AcoI YGGCCR 4 cut(s) 1050, 3016, 3490, 3766
AcsI RAATTY 8 cut(s) 1578, 1771, 2304, 2510, 3408, 3786, 4744, 4786
AcuI CTGAAG 5 cut(s) 543, 1814, 4020, 4392, 4468
AcvI CACGTG 1 cut(s) 71
AcyI GRCGYC 2 cut(s) 32, 4178
AfaI GTAC 8 cut(s) 174, 288, 367, 1291, 2163, 2339, 3531, 4542
AflIII ACRYGT 2 cut(s) 1679, 2920
AhlI ACTAGT 2 cut(s) 2633, 3631
AjiI CACGTC 1 cut(s) 1597
AjnI CCWGG 7 cut(s) 854, 862, 1369, 2278, 2721, 3108, 3796
AjuI GAANNNNNNNTTGG 8 cut(s) 423, 455, 1234, 1266, 1902, 1934, 3973, 4005
Alw21I GWGCWC 3 cut(s) 1264, 1718, 3949
Alw26I GTCTC 6 cut(s) 853, 1353, 1667, 2403, 2463, 3244
AlwNI CAGNNNCTG 1 cut(s) 284
Ama87I CYCGRG 1 cut(s) 3278
Aor13HI TCCGGA 1 cut(s) 4702
AoxI GGCC 9 cut(s) 1050, 1361, 2719, 3016, 3273, 3490, 3766, 3934, 4397
ApoI RAATTY 8 cut(s) 1578, 1771, 2304, 2510, 3408, 3786, 4744, 4786
ArsI GACNNNNNNTTYG 4 cut(s) 2015, 2047, 2462, 2494
AseI ATTAAT 2 cut(s) 1455, 3917
Asp718I GGTACC 1 cut(s) 2337
AspLEI GCGC 7 cut(s) 270, 325, 874, 1954, 2251, 4324, 4474
AsuC2I CCSGG 3 cut(s) 2016, 3501, 3569
AsuHPI GGTGA 8 cut(s) 465, 606, 1161, 1293, 1580, 2778, 3205, 4020
AsuII TTCGAA 2 cut(s) 500, 3209
AvaI CYCGRG 1 cut(s) 3278
AvaII GGWCC 9 cut(s) 811, 2501, 3031, 3075, 3218, 4371, 4445, 4606, 4628
AxyI CCTNAGG 1 cut(s) 2943
BaeGI GKGCMC 5 cut(s) 945, 2889, 3596, 3712, 4019
BalI TGGCCA 3 cut(s) 1052, 3492, 3768
BamHI GGATCC 2 cut(s) 3793, 4267
BanI GGYRCC 2 cut(s) 2337, 2886
BanII GRGCYC 3 cut(s) 1264, 1718, 3949
BarI GAAGNNNNNNTAC 4 cut(s) 3299, 3331, 4066, 4098
BauI CACGAG 2 cut(s) 2568, 4018
BbrPI CACGTG 1 cut(s) 71
BbsI GAAGAC 1 cut(s) 695
Bbv12I GWGCWC 3 cut(s) 1264, 1718, 3949
BceAI ACGGC 3 cut(s) 385, 675, 3031
BcgI CGANNNNNNTGC 4 cut(s) 3311, 3345, 3593, 3627
BciT130I CCWGG 7 cut(s) 856, 864, 1371, 2280, 2723, 3110, 3798
BciVI GTATCC 3 cut(s) 382, 1751, 3318
BclI TGATCA 1 cut(s) 2587
BcnI CCSGG 3 cut(s) 2016, 3501, 3569
BcoDI GTCTC 6 cut(s) 853, 1353, 1667, 2403, 2463, 3244
BcuI ACTAGT 2 cut(s) 2633, 3631
BfmI CTRYAG 6 cut(s) 236, 369, 1342, 2027, 3008, 4686
BfoI RGCGCY 1 cut(s) 875
BfuAI ACCTGC 4 cut(s) 2146, 3607, 4014, 4039
BfuI GTATCC 3 cut(s) 382, 1751, 3318
BglII AGATCT 1 cut(s) 4579
BlpI GCTNAGC 1 cut(s) 4137
Bme18I GGWCC 9 cut(s) 811, 2501, 3031, 3075, 3218, 4371, 4445, 4606, 4628
BmeT110I CYCGRG 1 cut(s) 3278
BmgBI CACGTC 1 cut(s) 1597
BmrI ACTGGG 3 cut(s) 394, 3341, 3635
BmsI GCATC 9 cut(s) 1648, 2572, 2868, 3376, 3676, 3872, 4064, 4446, 4777
BmuI ACTGGG 3 cut(s) 394, 3341, 3635
BoxI GACNNNNGTC 1 cut(s) 22
BpiI GAAGAC 1 cut(s) 695
BpmI CTGGAG 3 cut(s) 877, 2016, 4193
Bpu1102I GCTNAGC 1 cut(s) 4137
Bpu14I TTCGAA 2 cut(s) 500, 3209
BpuEI CTTGAG 4 cut(s) 1180, 1341, 2947, 4269
BpuMI CCSGG 3 cut(s) 2016, 3501, 3569
BsaAI YACGTR 3 cut(s) 71, 1682, 1778
BsaBI GATNNNNATC 1 cut(s) 2586
BsaHI GRCGYC 2 cut(s) 32, 4178
BsaI GGTCTC 3 cut(s) 853, 1353, 2403
BsaJI CCNNGG 7 cut(s) 27, 326, 1563, 1947, 2341, 3277, 4011
BsaWI WCCGGW 2 cut(s) 4630, 4702
BsaXI ACNNNNNCTCC 6 cut(s) 349, 379, 1134, 1164, 4617, 4647
Bse118I RCCGGY 1 cut(s) 2844
Bse21I CCTNAGG 1 cut(s) 2943
Bse3DI GCAATG 9 cut(s) 314, 465, 1084, 1202, 2181, 2205, 3653, 3937, 4422
Bse8I GATNNNNATC 1 cut(s) 2586
BseAI TCCGGA 1 cut(s) 4702
BseBI CCWGG 7 cut(s) 856, 864, 1371, 2280, 2723, 3110, 3798
BseDI CCNNGG 7 cut(s) 27, 326, 1563, 1947, 2341, 3277, 4011
BseGI GGATG 9 cut(s) 907, 1912, 1929, 2983, 3245, 3863, 3983, 4528, 4768
BseJI GATNNNNATC 1 cut(s) 2586
BseMI GCAATG 9 cut(s) 314, 465, 1084, 1202, 2181, 2205, 3653, 3937, 4422
BseMII CTCAG 9 cut(s) 1731, 2277, 2424, 2934, 3549, 3752, 3824, 4151, 4408
BseSI GKGCMC 5 cut(s) 945, 2889, 3596, 3712, 4019
BseYI CCCAGC 2 cut(s) 581, 2775
BsgI GTGCAG 1 cut(s) 3923
Bsh1236I CGCG 4 cut(s) 46, 270, 2922, 4183
BshFI GGCC 9 cut(s) 1052, 1363, 2721, 3018, 3275, 3492, 3768, 3936, 4399
BshNI GGYRCC 2 cut(s) 2337, 2886
BsiHKAI GWGCWC 3 cut(s) 1264, 1718, 3949
BsiHKCI CYCGRG 1 cut(s) 3278
BsiSI CCGG 6 cut(s) 2015, 2845, 3501, 3569, 4631, 4703
BslFI GGGAC 6 cut(s) 388, 824, 1114, 3204, 3642, 4384
BsmAI GTCTC 6 cut(s) 853, 1353, 1667, 2403, 2463, 3244
BsmFI GGGAC 6 cut(s) 388, 824, 1114, 3204, 3642, 4384
BsmI GAATGC 4 cut(s) 508, 1648, 3776, 4430
BsnI GGCC 9 cut(s) 1052, 1363, 2721, 3018, 3275, 3492, 3768, 3936, 4399
Bso31I GGTCTC 3 cut(s) 853, 1353, 2403
BsoBI CYCGRG 1 cut(s) 3278
Bsp119I TTCGAA 2 cut(s) 500, 3209
Bsp1286I GDGCHC 8 cut(s) 945, 1264, 1718, 2889, 3596, 3712, 3949, 4019
Bsp13I TCCGGA 1 cut(s) 4702
Bsp1720I GCTNAGC 1 cut(s) 4137
Bsp19I CCATGG 1 cut(s) 1947
BspANI GGCC 9 cut(s) 1052, 1363, 2721, 3018, 3275, 3492, 3768, 3936, 4399
BspCNI CTCAG 9 cut(s) 1730, 2276, 2425, 2935, 3550, 3751, 3823, 4150, 4407
BspEI TCCGGA 1 cut(s) 4702
BspFNI CGCG 4 cut(s) 46, 270, 2922, 4183
BspMAI CTGCAG 3 cut(s) 240, 3012, 4690
BspMI ACCTGC 4 cut(s) 2146, 3607, 4014, 4039
BspQI GCTCTTC 1 cut(s) 4675
BspT104I TTCGAA 2 cut(s) 500, 3209
BspT107I GGYRCC 2 cut(s) 2337, 2886
BspTNI GGTCTC 3 cut(s) 853, 1353, 2403
BsrDI GCAATG 9 cut(s) 314, 465, 1084, 1202, 2181, 2205, 3653, 3937, 4422
BsrFI RCCGGY 1 cut(s) 2844
BssAI RCCGGY 1 cut(s) 2844
BssECI CCNNGG 7 cut(s) 27, 326, 1563, 1947, 2341, 3277, 4011
BssNI GRCGYC 2 cut(s) 32, 4178
BssSI CACGAG 2 cut(s) 2568, 4018
BssT1I CCWWGG 3 cut(s) 1947, 2341, 4011
Bst2BI CACGAG 2 cut(s) 2568, 4018
Bst2UI CCWGG 7 cut(s) 856, 864, 1371, 2280, 2723, 3110, 3798
Bst4CI ACNGT 5 cut(s) 1972, 2031, 2318, 2758, 4224
Bst6I CTCTTC 5 cut(s) 95, 2685, 3855, 4487, 4675
BstACI GRCGYC 2 cut(s) 32, 4178
BstAPI GCANNNNNTGC 1 cut(s) 464
BstBAI YACGTR 3 cut(s) 71, 1682, 1778
BstBI TTCGAA 2 cut(s) 500, 3209
BstDSI CCRYGG 2 cut(s) 326, 1947
BstEII GGTNACC 2 cut(s) 1568, 4026
BstENI CCTNNNNNAGG 3 cut(s) 1356, 1562, 4352
BstF5I GGATG 9 cut(s) 907, 1912, 1929, 2983, 3245, 3863, 3983, 4528, 4768
BstFNI CGCG 4 cut(s) 46, 270, 2922, 4183
BstH2I RGCGCY 1 cut(s) 875
BstHHI GCGC 7 cut(s) 270, 325, 874, 1954, 2251, 4324, 4474
BstMAI GTCTC 6 cut(s) 853, 1353, 1667, 2403, 2463, 3244
BstNI CCWGG 7 cut(s) 856, 864, 1371, 2280, 2723, 3110, 3798
BstNSI RCATGY 4 cut(s) 1283, 3051, 4089, 4122
BstPAI GACNNNNGTC 1 cut(s) 22
BstPI GGTNACC 2 cut(s) 1568, 4026
BstSFI CTRYAG 6 cut(s) 236, 369, 1342, 2027, 3008, 4686
BstSLI GKGCMC 5 cut(s) 945, 2889, 3596, 3712, 4019
BstSNI TACGTA 1 cut(s) 1778
BstUI CGCG 4 cut(s) 46, 270, 2922, 4183
BstV2I GAAGAC 1 cut(s) 695
BstX2I RGATCY 8 cut(s) 851, 1026, 1686, 1720, 3546, 3793, 4267, 4579
BstXI CCANNNNNNTGG 2 cut(s) 4121, 4359
BstYI RGATCY 8 cut(s) 851, 1026, 1686, 1720, 3546, 3793, 4267, 4579
Bsu36I CCTNAGG 1 cut(s) 2943
BsuI GTATCC 3 cut(s) 382, 1751, 3318
BsuRI GGCC 9 cut(s) 1052, 1363, 2721, 3018, 3275, 3492, 3768, 3936, 4399
BtgI CCRYGG 2 cut(s) 326, 1947
BtrI CACGTC 1 cut(s) 1597
BtsCI GGATG 9 cut(s) 907, 1912, 1929, 2983, 3245, 3863, 3983, 4528, 4768
BtsI GCAGTG 1 cut(s) 2069
BtsIMutI CAGTG 7 cut(s) 567, 2069, 2754, 3113, 3229, 4209, 4407
BveI ACCTGC 4 cut(s) 2146, 3607, 4014, 4039
CaiI CAGNNNCTG 1 cut(s) 284
CfoI GCGC 7 cut(s) 270, 325, 874, 1954, 2251, 4324, 4474
Cfr10I RCCGGY 1 cut(s) 2844
CseI GACGC 5 cut(s) 21, 259, 2911, 4167, 4688
Csp6I GTAC 8 cut(s) 173, 287, 366, 1290, 2162, 2338, 3530, 4541
CviQI GTAC 8 cut(s) 173, 287, 366, 1290, 2162, 2338, 3530, 4541
DraI TTTAAA 3 cut(s) 753, 2115, 3693
DrdI GACNNNNNNGTC 1 cut(s) 4247
DseDI GACNNNNNNGTC 1 cut(s) 4247
EaeI YGGCCR 4 cut(s) 1050, 3016, 3490, 3766
Eam1104I CTCTTC 5 cut(s) 95, 2685, 3855, 4487, 4675
EarI CTCTTC 5 cut(s) 95, 2685, 3855, 4487, 4675
EciI GGCGGA 3 cut(s) 347, 2762, 4011
Ecl136II GAGCTC 3 cut(s) 1262, 1716, 3947
Eco105I TACGTA 1 cut(s) 1778
Eco130I CCWWGG 3 cut(s) 1947, 2341, 4011
Eco24I GRGCYC 3 cut(s) 1264, 1718, 3949
Eco31I GGTCTC 3 cut(s) 853, 1353, 2403
Eco47I GGWCC 9 cut(s) 811, 2501, 3031, 3075, 3218, 4371, 4445, 4606, 4628
Eco53kI GAGCTC 3 cut(s) 1262, 1716, 3947
Eco57I CTGAAG 5 cut(s) 543, 1814, 4020, 4392, 4468
Eco72I CACGTG 1 cut(s) 71
Eco81I CCTNAGG 1 cut(s) 2943
Eco88I CYCGRG 1 cut(s) 3278
Eco91I GGTNACC 2 cut(s) 1568, 4026
EcoICRI GAGCTC 3 cut(s) 1262, 1716, 3947
EcoNI CCTNNNNNAGG 3 cut(s) 1356, 1562, 4352
EcoO109I RGGNCCY 6 cut(s) 1361, 2719, 3031, 3218, 3274, 4371
EcoO65I GGTNACC 2 cut(s) 1568, 4026
EcoRI GAATTC 4 cut(s) 2510, 3408, 4744, 4786
EcoRII CCWGG 7 cut(s) 854, 862, 1369, 2278, 2721, 3108, 3796
EcoT14I CCWWGG 3 cut(s) 1947, 2341, 4011
EcoT22I ATGCAT 1 cut(s) 4057
EcoT38I GRGCYC 3 cut(s) 1264, 1718, 3949
ErhI CCWWGG 3 cut(s) 1947, 2341, 4011
FalI AAGNNNNNCTT 4 cut(s) 1458, 1490, 3442, 3474
FaqI GGGAC 6 cut(s) 388, 824, 1114, 3204, 3642, 4384
FauI CCCGC 2 cut(s) 73, 550
FauNDI CATATG 3 cut(s) 786, 2806, 3576
FbaI TGATCA 1 cut(s) 2587
FblI GTMKAC 2 cut(s) 3163, 4126
FokI GGATG 9 cut(s) 914, 1919, 1936, 2990, 3232, 3850, 3970, 4535, 4755
FriOI GRGCYC 3 cut(s) 1264, 1718, 3949
FspAI RTGCGCAY 1 cut(s) 4473
FspI TGCGCA 1 cut(s) 4473
GlaI GCGC 7 cut(s) 269, 324, 873, 1953, 2250, 4323, 4473
GsaI CCCAGC 2 cut(s) 585, 2779
GsuI CTGGAG 3 cut(s) 877, 2016, 4193
HaeII RGCGCY 1 cut(s) 875
HaeIII GGCC 9 cut(s) 1052, 1363, 2721, 3018, 3275, 3492, 3768, 3936, 4399
HapII CCGG 6 cut(s) 2015, 2845, 3501, 3569, 4631, 4703
HgaI GACGC 5 cut(s) 21, 259, 2911, 4167, 4688
HhaI GCGC 7 cut(s) 270, 325, 874, 1954, 2251, 4324, 4474
Hin1I GRCGYC 2 cut(s) 32, 4178
Hin6I GCGC 7 cut(s) 268, 323, 872, 1952, 2249, 4322, 4472
HinP1I GCGC 7 cut(s) 268, 323, 872, 1952, 2249, 4322, 4472
HincII GTYRAC 3 cut(s) 922, 1440, 3541
HindII GTYRAC 3 cut(s) 922, 1440, 3541
HindIII AAGCTT 1 cut(s) 3506
HpaII CCGG 6 cut(s) 2015, 2845, 3501, 3569, 4631, 4703
HphI GGTGA 8 cut(s) 465, 606, 1161, 1293, 1580, 2778, 3205, 4020
Hpy166II GTNNAC 8 cut(s) 173, 366, 922, 1440, 1540, 3164, 3541, 4127
Hpy8I GTNNAC 8 cut(s) 173, 366, 922, 1440, 1540, 3164, 3541, 4127
Hpy99I CGWCG 2 cut(s) 275, 4183
HpyCH4III ACNGT 5 cut(s) 1972, 2031, 2318, 2758, 4224
HpyCH4IV ACGT 7 cut(s) 70, 1596, 1681, 1777, 2404, 4199, 4539
HpySE526I ACGT 7 cut(s) 70, 1596, 1681, 1777, 2404, 4199, 4539
Hsp92I GRCGYC 2 cut(s) 32, 4178
HspAI GCGC 7 cut(s) 268, 323, 872, 1952, 2249, 4322, 4472
KflI GGGWCCC 1 cut(s) 4371
Kpn2I TCCGGA 1 cut(s) 4702
KpnI GGTACC 1 cut(s) 2341
Ksp22I TGATCA 1 cut(s) 2587
LguI GCTCTTC 1 cut(s) 4675
LmnI GCTCC 7 cut(s) 357, 1713, 3111, 3944, 3952, 3974, 4458
LweI GCATC 9 cut(s) 1648, 2572, 2868, 3376, 3676, 3872, 4064, 4446, 4777
MaeII ACGT 7 cut(s) 70, 1596, 1681, 1777, 2404, 4199, 4539
MfeI CAATTG 4 cut(s) 78, 1410, 1917, 4094
MflI RGATCY 8 cut(s) 851, 1026, 1686, 1720, 3546, 3793, 4267, 4579
MhlI GDGCHC 8 cut(s) 945, 1264, 1718, 2889, 3596, 3712, 3949, 4019
MlsI TGGCCA 3 cut(s) 1052, 3492, 3768
MluI ACGCGT 1 cut(s) 2920
MluNI TGGCCA 3 cut(s) 1052, 3492, 3768
MlyI GAGTC 8 cut(s) 460, 1096, 2015, 3169, 3569, 3718, 4235, 4546
MmeI TCCRAC 4 cut(s) 2528, 2982, 4245, 4295
Mox20I TGGCCA 3 cut(s) 1052, 3492, 3768
Mph1103I ATGCAT 1 cut(s) 4057
MroI TCCGGA 1 cut(s) 4702
MscI TGGCCA 3 cut(s) 1052, 3492, 3768
MslI CAYNNNNRTG 3 cut(s) 1058, 1064, 4357
Msp20I TGGCCA 3 cut(s) 1052, 3492, 3768
MspA1I CMGCKG 6 cut(s) 38, 712, 2795, 3401, 3814, 4141
MspI CCGG 6 cut(s) 2015, 2845, 3501, 3569, 4631, 4703
MunI CAATTG 4 cut(s) 78, 1410, 1917, 4094
Mva1269I GAATGC 4 cut(s) 508, 1648, 3776, 4430
MvaI CCWGG 7 cut(s) 856, 864, 1371, 2280, 2723, 3110, 3798
MvnI CGCG 4 cut(s) 46, 270, 2922, 4183
NciI CCSGG 3 cut(s) 2016, 3501, 3569
NcoI CCATGG 1 cut(s) 1947
NdeI CATATG 3 cut(s) 786, 2806, 3576
NmeAIII GCCGAG 2 cut(s) 8, 962
NsbI TGCGCA 1 cut(s) 4473
NsiI ATGCAT 1 cut(s) 4057
NspI RCATGY 4 cut(s) 1283, 3051, 4089, 4122
NspV TTCGAA 2 cut(s) 500, 3209
PaeI GCATGC 1 cut(s) 1283
PaqCI CACCTGC 2 cut(s) 3607, 4039
PciSI GCTCTTC 1 cut(s) 4675
PcsI WCGNNNNNNNCGW 1 cut(s) 29
PctI GAATGC 4 cut(s) 508, 1648, 3776, 4430
PflFI GACNNNGTC 1 cut(s) 4495
PflMI CCANNNNNTGG 2 cut(s) 1208, 4603
PfoI TCCNGGA 2 cut(s) 854, 3499
PleI GAGTC 8 cut(s) 459, 1096, 2014, 3168, 3568, 3718, 4235, 4546
PmaCI CACGTG 1 cut(s) 71
PmlI CACGTG 1 cut(s) 71
PpsI GAGTC 8 cut(s) 459, 1096, 2014, 3168, 3568, 3718, 4235, 4546
Ppu21I YACGTR 3 cut(s) 71, 1682, 1778
PpuMI RGGWCCY 3 cut(s) 3031, 3218, 4371
PshAI GACNNNNGTC 1 cut(s) 22
PshBI ATTAAT 2 cut(s) 1455, 3917
Psp124BI GAGCTC 3 cut(s) 1264, 1718, 3949
Psp5II RGGWCCY 3 cut(s) 3031, 3218, 4371
Psp6I CCWGG 7 cut(s) 854, 862, 1369, 2278, 2721, 3108, 3796
PspCI CACGTG 1 cut(s) 71
PspEI GGTNACC 2 cut(s) 1568, 4026
PspFI CCCAGC 2 cut(s) 581, 2775
PspGI CCWGG 7 cut(s) 854, 862, 1369, 2278, 2721, 3108, 3796
PspPPI RGGWCCY 3 cut(s) 3031, 3218, 4371
PstI CTGCAG 3 cut(s) 240, 3012, 4690
PstNI CAGNNNCTG 1 cut(s) 284
PsuI RGATCY 8 cut(s) 851, 1026, 1686, 1720, 3546, 3793, 4267, 4579
PsyI GACNNNGTC 1 cut(s) 4495
PvuII CAGCTG 5 cut(s) 38, 712, 3401, 3814, 4141
RsaI GTAC 8 cut(s) 174, 288, 367, 1291, 2163, 2339, 3531, 4542
RsaNI GTAC 8 cut(s) 173, 287, 366, 1290, 2162, 2338, 3530, 4541
RseI CAYNNNNRTG 3 cut(s) 1058, 1064, 4357
SacI GAGCTC 3 cut(s) 1264, 1718, 3949
SapI GCTCTTC 1 cut(s) 4675
SchI GAGTC 8 cut(s) 460, 1096, 2015, 3169, 3569, 3718, 4235, 4546
SduI GDGCHC 8 cut(s) 945, 1264, 1718, 2889, 3596, 3712, 3949, 4019
SfaNI GCATC 9 cut(s) 1648, 2572, 2868, 3376, 3676, 3872, 4064, 4446, 4777
SfcI CTRYAG 6 cut(s) 236, 369, 1342, 2027, 3008, 4686
SfuI TTCGAA 2 cut(s) 500, 3209
SinI GGWCC 9 cut(s) 811, 2501, 3031, 3075, 3218, 4371, 4445, 4606, 4628
SmiMI CAYNNNNRTG 3 cut(s) 1058, 1064, 4357
SmlI CTYRAG 4 cut(s) 1159, 1356, 2926, 4284
SmoI CTYRAG 4 cut(s) 1159, 1356, 2926, 4284
SnaBI TACGTA 1 cut(s) 1778
SpeI ACTAGT 2 cut(s) 2633, 3631
SphI GCATGC 1 cut(s) 1283
SspI AATATT 3 cut(s) 3203, 3522, 4315
SstI GAGCTC 3 cut(s) 1264, 1718, 3949
StyI CCWWGG 3 cut(s) 1947, 2341, 4011
TaaI ACNGT 5 cut(s) 1972, 2031, 2318, 2758, 4224
TaiI ACGT 7 cut(s) 73, 1599, 1684, 1780, 2407, 4202, 4542
TatI WGTACW 5 cut(s) 172, 286, 365, 1289, 3529
TauI GCSGC 2 cut(s) 77, 120
TscAI CASTG 7 cut(s) 567, 2076, 2761, 3120, 3236, 4216, 4407
TspGWI ACGGA 5 cut(s) 1287, 2719, 3957, 3961, 4437
TspRI CASTG 7 cut(s) 567, 2076, 2761, 3120, 3236, 4216, 4407
Tth111I GACNNNGTC 1 cut(s) 4495
Van91I CCANNNNNTGG 2 cut(s) 1208, 4603
VpaK11BI GGWCC 9 cut(s) 811, 2501, 3031, 3075, 3218, 4371, 4445, 4606, 4628
VspI ATTAAT 2 cut(s) 1455, 3917
XagI CCTNNNNNAGG 3 cut(s) 1356, 1562, 4352
XapI RAATTY 8 cut(s) 1578, 1771, 2304, 2510, 3408, 3786, 4744, 4786
XceI RCATGY 4 cut(s) 1283, 3051, 4089, 4122
XcmI CCANNNNNNNNNTGG 1 cut(s) 821
XmiI GTMKAC 2 cut(s) 3163, 4126
Zsp2I ATGCAT 1 cut(s) 4057
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.