FvH4_4g33860

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009426
Physical Location & Seq
Reverse (-)
31910781 .. 31921230
10450 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g33860.t2

Sequence Viewer

Length: 4806 bp
ATGGATCAAAACCAGAGATCATCATCGTCCGCTTCAGCTTCCCGAGCTTACCAGTTCCACCCTGCTCGTGCCGCCATCGTCCATCTCTTCGACCTCTACTTAGGAAAGAGTAACCGCCTGAAATCGGAGGATTCAATTCCCGAGCCTCCGAACAAGTCACAGAAGCGTGTGCTTGCTCTCAACAGAGAGCTTCCTCCTCGAAACGAGCAGTTCCTTCTCGATTTCGAGCAGCTCCAGAGCCAGTTTCCTGATCAAGATCAGCTGCGTGTTGTGACAGAGTCGGTGCTTATATCTCTAGTTGTGCAATGCAGCAATCATGCACCACGCGCGGAGTTTCTTCTGTTTGCTTTAAGGAGTTTGTGCACTATAGGTCACATTAACTGGGACACTTTTTTGCCGGCCCTGCTTTCGTCAGTCTCAACTGCAGAGATGTCGATGGGTCAAGGGAGCCAGGCAATGGCTGGTGTTTCATCACAGTCCAGCATGCTTCCAACTTCAAATACGATTCAGAATTCATCTAATTTTCAGTCTTCAAATCCTGCCTCTCCATTACCTTCTGTTCATGGTATTGGCTCCCCCGGCCAGTCAGCTATGGAAACTATGACTGTCTCGCCTGCTAAATCATCTGATATGCCCAGTAGCGGACAGCAGGCTGCAGCAAGGGCCAATACATCTATAAGAGATAATGCTATCAGCAGTTTACGTCAGTTGTGTTGCAAAATTATTTTGACTGGACTTGGATTTAATTTAAAACCTGTAACTCATGCCGACATTTTTTCTCATATGCTGAATTGGCTGGTTAATTGGGATCAAAAACAACCGGGGACTGATGAATCTGATGGCGTAAAATCTTGGAGATCTGGGAAGGCACTAATTGAATGGCTGCATAGCTGTTTGGATGTTATTTGGCTTTTGGTTGACGAGGAAAAATGCCGAGTTCCCTTCTATGAATTACTCCGCAGTGGCTTGCAATTTATGGAGAACATACCTGATGATGAAGCCCTGTTTACTCTAATCTTAGAGATCCACAGGAGGCGAGATATGATGGCTATGCACATGAAAATGCTTGATCAACACTTGCATTGCCCTTCATTTGGGACTCATCGAATTTTCCCGCAGACTACTCCTAGTATATCAGGTGAAGCAGTGGCAAGCTTGCGGTATTCACCAATCACATATCCAAGTGTACTTGGAGAACCATTGCATGGAGAGGACCTTGCAATTTCTATTCCAAAAGGAAGTTTAGATTGGGAGAGAGCATTGCGGTGTATAAGGCATGCTATTTGTACTACTCCATCACCTGACTGGTGGAAACGTGTGCTGCTTGTGGCTCCTTGTTATAGGGGTCCTTCTCAAGGTCCTACTCCTGGTGCTGTTTTCACTTCTGAAATGATTTGTGAGGCAACAATTGATAGAATTGTTGAGCTACTGAAGTTGACAAATTCAGATGTTAATTGCTGGCAAGACTGGCTTGTCTTTTCAGATATATTCTTTTTTCTTATAAAAAGCGGCTGTGTTGATTTCGTCTATTTTGTCGGCAAACTAGTTTCACGCCTTACAGAGAGTGATCCCCATATTCTTAGGACCAATCATGTCACTTGGCTTCTAGCTCAAATTATTCGGGTTGAGCTTGTGATAAATGCTCTAAATTCAGATGCTAGAAAGGTAGAAACAACTCGGAAGATCCTATCGCTTCATAAAGAAGATAGAAACTCAGATCCAAATAGTCCTCAAAGTATACTGCTTGACTTTATAAGCAGTTGCCAAAATTTACGTATTTGGTCCCTGAACACAACAACTAGAGAATATCTGAATAATGAGCAGCTTCAGAAAGGAAAGGCAATAGACGAATGGTGGAGACATGCAAGCAAAGGGGATCGTATGATGGATTATATGAATATGGATGATAAGTCAATTGGGATGTTTTGGGTTGTATCTTACACCATGGCGCAACCAGCTTGTGAAACTGTGATCAATTGGTTATCCTCTGCTGGAGTTGCAGAATCGTTACCAGCAACAAACCTACAGTCCAACGAGAGGTTAATGGTTATGCGTGAAGTAAATCCATTGCCGATGTCATTGTTATCTGGCTTTGCAATAAACCTATGTTTAAAGCTGGCATATCAAATGGAAGACTCTCTGTTTTGCGGGCAGGTTGTACCTAATATTGCAATGGCTGAAACTTATTGCAGGTTACTGCTCATTGCACCTCATTCACTATTCCGTTCTCATTTCAATCATTTGGCACAAAGGAGTCCTAATGTATTGAGCAAGCCTGGTGTAACTCTTTTGGTGCTTGAAATTTTGAACTATCGGTTGCTTCCACTTTACAGGTACCAGGGGAAAAGCAAAGCTTTGATGTATGATGTTACAAAAATAATATCTGCTCTGCAAAAAAAACGTGGAGACCATCGTGTATTTAGATTGGCTGAGAATTTGTGTATGAATCTTATTCTGTCACTGCGAGATTTTTTCTTAGTGAAAAGGGAAGGGAAGGGTCCAACTGAATTCACAGAAACTCTGAATCGAGCAACTGTGGTCACTCTTGCTATCATCATTAAGACAAGAGGAATTGCTGATGCTGATCATCTGCATTATCTTCAAACCATGTTAGAGCAGATATTAGAGAATAGTAATCATACATGGTCAGAGAAAACACTACGCTATTTCCCTTCGCTTCTTCGTGACCTGTTGATTCCTCGGATAGATAACAGGGGCATTGCAATTCAAGCATGGCAGCAGGCAGAAACCACTGTGATCAACCAATGCACTCAGCTTCTGTCGTCATCACCTGATCCTACTTATGTTATGACCTACATAAATAATAGTTTTTTTCAACACCGTAAATATCTATGTGCTGGAGCATGGATCCTGATGCAAGGGCACCCTGAGAATGTAAACAGTGTAAACTTGGCACGTGTCTTGCGAGAATTTTCCCCTGAGGAAGTAACAGCTAATATTTACATGATGGTGGATGTATTGCTTCATCACATTCGATTGGAACTTCAGCACGGGCATTCCTTGCAGGACCTTTTACTGAAAGCATGTGCAAACCTAACATTTTTCATTTGGACCCACGAGTTACTTCCTTTGGATATTATGCTTTTGGCACTTATTGATCGGGATGATGATCCCCATGCTTTGCGTATTGTGATTAGTTTACTTGATAGGCAAGAACTTCAACAAAGAGTGAAACTATATTGTATGAATCGAGGTGCCCCTGAGCACTGGCTTTACCCCGGACCCTTCGTACGAGTTGAATTGCAAAAGGCCCTTGGAAATCATCTTTCCTGGAAGGACAAGTATCCCACATTTTTCGATGACATTGCAGCGCGTTTGCTCCCGGTCATTCCATTAATAATCTATAGACTAATTGAAAATGATGCCATGGATTCAGCGGACAGAGTACTGGCCATCTATACTCCATTTCTTGCTTACCACCCTTTTAGATTTACATTTGTTCGTGACATTCTTGCATATTTCTACGGCCATCTTCCTGGAAAGCTTATTGTTCGAATCCTGAATGTACTCGATATCAGCAAGATTCCATTGTCTGAGTCATTTCCGCAGCACATCAATTCATCAAATCCTGTTATATGCCCACCTCCGGATTACTTTGCAACTCTCCTGCTGGGAATAGTGAATAATGTTATACCTCCGTTGCATAACAACTCAAAATCTGGATCAGCTAGTGATGCTTTAAACAACTCAATGCGTGCTCCACCTAACAAGACTCCAGCAACCTCTCAATCTAAGCAAACAAATGCTTCTGAGGGCCAAAAGTCATTTTATCAAATTCAGGATCCTGGCACATATACTCAGCTCGTTCTTGAAACAGCAGTCATTGAATTACTGTCTCTTCCGGTATCTGCATCCCAGATTGTATCTTCTCTTGTTCAAATTGTCATCAATATACAACCAACACTAATTCAATCCAGCAATGGCCTGCATGGAGCTACAAATGGTGTTGGGCAAGGCTCAGTATTACCAACATCCCCTTCAGGTGGCAGCACAGATTCCTTAGGCACAAACAGATCAAGTCCGTCAGTTTCAGGAATAAATGTTTCTAGCTTTGTTTCTCGGAGTGGTTATACGTGCCAACAATTGTCTTGCTTATTAATCCAAGCATGTGGTCATCTACTGGCTCAGCTCCCTCCTGATTTTCATGTACAACTCTATATAGAGGCATCACGTATAATAAAAGAAACTTGGTGGCTCACTGATGGCAAGAGATCACCGGGGGAACTAGACTCTGCTGTTGGTTATGCTTTGTTGGATCCAACGTGGGCTGCTCAAGACAACACCTCCACAGCTATTGGTAATATTGTTTCTCTGCTTCATTCATTTTTCAGTAACCTACCAATGGAATGGCTGGAAGGGACCCATCTTATCATCAAACATCTCAGGCCAGTGACATCAGTTGCAATGTTGAGAATAGTGTTCCGTATAATGGCTCCATTGCTCCCAAAGCTTGCCAATGCTCACAACCTCTTCAATAAGATCCTCTCATTGATTTTTAGTATGATGGTCGACGTCTTTGGGAAGAATGCACAGCCATCAACTCTTGTTGAGCCACTAGAAGTGACAGACCTTATAGACTTCTTCCATCATATTGTCCACTATGAAGGACAAGGAGGGCCTGTGCAGGCTAACAGCAAGCCGAGACCAGAGGTTTTAGTTCTGTGTGGAAGAGCTGCAGAAAGTCTACGCCCAGAGATACAGCATCTTCTTTTGCACTTGAAACCCGACACAAATTCTTCCATTTATGCTGCTACTCATCCTAAGTTGGCACAGAACACATCATAA

Protein Analysis

1602

Amino Acids

179.53

Weight (kDa)

6.73

Isoelectric Point (pI)

46.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med23 PF11573 490 - 1210 5.4e-25 Mediator complex subunit 23
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1502, 1754
AasI GACNNNNNNGTC 1 cut(s) 1472
AatII GACGTC 1 cut(s) 4536
Acc36I ACCTGC 2 cut(s) 2143, 2181
Acc65I GGTACC 1 cut(s) 2334
AccB1I GGYRCC 3 cut(s) 2334, 2883, 3215
AccB7I CCANNNNNTGG 2 cut(s) 457, 1205
AccI GTMKAC 3 cut(s) 1738, 4530, 4705
AccII CGCG 3 cut(s) 327, 329, 3334
AccIII TCCGGA 1 cut(s) 3607
AcoI YGGCCR 3 cut(s) 580, 3411, 3487
AcuI CTGAAG 5 cut(s) 18, 1451, 1811, 2990, 3984
AcvI CACGTG 1 cut(s) 2918
AcyI GRCGYC 1 cut(s) 4533
AfaI GTAC 8 cut(s) 1188, 1288, 2160, 2336, 3252, 3408, 3528, 4170
AflIII ACRYGT 2 cut(s) 1315, 2917
AhlI ACTAGT 1 cut(s) 1543
AjnI CCWGG 7 cut(s) 450, 1366, 2275, 2337, 3290, 3496, 3805
AjuI GAANNNNNNNTTGG 4 cut(s) 1231, 1263, 3982, 4014
Alw21I GWGCWC 3 cut(s) 365, 3228, 3721
Alw26I GTCTC 6 cut(s) 421, 613, 1852, 2400, 3861, 4657
Alw44I GTGCAC 1 cut(s) 361
AlwNI CAGNNNCTG 1 cut(s) 2779
Ama87I CYCGRG 2 cut(s) 42, 140
Aor13HI TCCGGA 1 cut(s) 3607
ApaLI GTGCAC 1 cut(s) 361
ArsI GACNNNNNNTTYG 2 cut(s) 2012, 2044
AseI ATTAAT 2 cut(s) 3356, 4118
Asp718I GGTACC 1 cut(s) 2334
AspLEI GCGC 3 cut(s) 329, 1951, 3334
AsuC2I CCSGG 5 cut(s) 579, 822, 3240, 3344, 4239
AsuHPI GGTGA 5 cut(s) 1151, 1158, 1290, 2781, 4227
AsuII TTCGAA 1 cut(s) 3514
AvaI CYCGRG 2 cut(s) 42, 140
AxyI CCTNAGG 2 cut(s) 2940, 4021
BaeGI GKGCMC 3 cut(s) 365, 2886, 3220
BalI TGGCCA 1 cut(s) 3413
BamHI GGATCC 3 cut(s) 2868, 3802, 4276
BanI GGYRCC 3 cut(s) 2334, 2883, 3215
BauI CACGAG 2 cut(s) 66, 3077
BbrPI CACGTG 1 cut(s) 2918
BbsI GAAGAC 2 cut(s) 522, 2139
Bbv12I GWGCWC 3 cut(s) 365, 3228, 3721
BceAI ACGGC 1 cut(s) 3502
BcgI CGANNNNNNTGC 4 cut(s) 414, 448, 3308, 3342
BciT130I CCWGG 7 cut(s) 452, 1368, 2277, 2339, 3292, 3498, 3807
BciVI GTATCC 1 cut(s) 3315
BclI TGATCA 5 cut(s) 250, 1069, 1971, 2584, 2757
BcnI CCSGG 5 cut(s) 579, 822, 3240, 3344, 4239
BcoDI GTCTC 6 cut(s) 421, 613, 1852, 2400, 3861, 4657
BcuI ACTAGT 1 cut(s) 1543
BfmI CTRYAG 6 cut(s) 366, 423, 654, 2024, 3364, 4695
BfuAI ACCTGC 2 cut(s) 2143, 2181
BfuI GTATCC 1 cut(s) 3315
BglII AGATCT 1 cut(s) 857
BlpI GCTNAGC 1 cut(s) 4146
BmcAI AGTACT 1 cut(s) 3408
BmeT110I CYCGRG 2 cut(s) 42, 140
BmrI ACTGGG 2 cut(s) 391, 630
BmsI GCATC 8 cut(s) 1645, 2569, 2865, 3373, 3685, 3881, 4196, 4732
BmuI ACTGGG 2 cut(s) 391, 630
BpiI GAAGAC 2 cut(s) 522, 2139
BpmI CTGGAG 4 cut(s) 218, 2013, 2880, 3720
Bpu10I CCTNAGC 1 cut(s) 3222
Bpu1102I GCTNAGC 1 cut(s) 4146
Bpu14I TTCGAA 1 cut(s) 3514
BpuEI CTTGAG 2 cut(s) 1338, 4278
BpuMI CCSGG 5 cut(s) 579, 822, 3240, 3344, 4239
BsaAI YACGTR 4 cut(s) 1775, 2918, 4095, 4193
BsaBI GATNNNNATC 4 cut(s) 22, 255, 2583, 3129
BsaHI GRCGYC 1 cut(s) 4533
BsaI GGTCTC 2 cut(s) 2400, 4657
BsaJI CCNNGG 9 cut(s) 577, 821, 1944, 2338, 2699, 3238, 3274, 3387, 4238
BsaWI WCCGGW 2 cut(s) 3607, 3862
BsaXI ACNNNNNCTCC 4 cut(s) 4289, 4319, 4626, 4656
Bse118I RCCGGY 1 cut(s) 397
Bse21I CCTNAGG 2 cut(s) 2940, 4021
Bse8I GATNNNNATC 4 cut(s) 22, 255, 2583, 3129
BseAI TCCGGA 1 cut(s) 3607
BseBI CCWGG 7 cut(s) 452, 1368, 2277, 2339, 3292, 3498, 3807
BseDI CCNNGG 9 cut(s) 577, 821, 1944, 2338, 2699, 3238, 3274, 3387, 4238
BseGI GGATG 8 cut(s) 904, 1909, 1926, 2980, 3130, 3872, 3992, 4777
BseJI GATNNNNATC 4 cut(s) 22, 255, 2583, 3129
BseRI GAGGAG 1 cut(s) 186
BseSI GKGCMC 3 cut(s) 365, 2886, 3220
BseYI CCCAGC 1 cut(s) 3631
BsgI GTGCAG 1 cut(s) 4664
Bsh1236I CGCG 3 cut(s) 327, 329, 3334
BshNI GGYRCC 3 cut(s) 2334, 2883, 3215
BsiHKAI GWGCWC 3 cut(s) 365, 3228, 3721
BsiHKCI CYCGRG 2 cut(s) 42, 140
BsiSI CCGG 8 cut(s) 398, 579, 821, 3240, 3344, 3608, 3863, 4238
BsiWI CGTACG 1 cut(s) 3250
BslFI GGGAC 5 cut(s) 398, 838, 1111, 1768, 4393
BsmAI GTCTC 6 cut(s) 421, 613, 1852, 2400, 3861, 4657
BsmFI GGGAC 5 cut(s) 398, 838, 1111, 1768, 4393
BsmI GAATGC 2 cut(s) 3016, 4552
Bso31I GGTCTC 2 cut(s) 2400, 4657
BsoBI CYCGRG 2 cut(s) 42, 140
Bsp119I TTCGAA 1 cut(s) 3514
Bsp1286I GDGCHC 5 cut(s) 365, 2886, 3220, 3228, 3721
Bsp13I TCCGGA 1 cut(s) 3607
Bsp1407I TGTACA 1 cut(s) 4168
Bsp1720I GCTNAGC 1 cut(s) 4146
Bsp19I CCATGG 2 cut(s) 1944, 3387
BspEI TCCGGA 1 cut(s) 3607
BspFNI CGCG 3 cut(s) 327, 329, 3334
BspMAI CTGCAG 3 cut(s) 427, 658, 4699
BspMI ACCTGC 2 cut(s) 2143, 2181
BspQI GCTCTTC 1 cut(s) 4684
BspT104I TTCGAA 1 cut(s) 3514
BspT107I GGYRCC 3 cut(s) 2334, 2883, 3215
BspTNI GGTCTC 2 cut(s) 2400, 4657
BsrFI RCCGGY 1 cut(s) 397
BsrGI TGTACA 1 cut(s) 4168
BssAI RCCGGY 1 cut(s) 397
BssECI CCNNGG 9 cut(s) 577, 821, 1944, 2338, 2699, 3238, 3274, 3387, 4238
BssNAI GTATAC 1 cut(s) 1739
BssNI GRCGYC 1 cut(s) 4533
BssSI CACGAG 2 cut(s) 66, 3077
BssT1I CCWWGG 3 cut(s) 1944, 3274, 3387
Bst1107I GTATAC 1 cut(s) 1739
Bst2BI CACGAG 2 cut(s) 66, 3077
Bst2UI CCWGG 7 cut(s) 452, 1368, 2277, 2339, 3292, 3498, 3807
Bst4CI ACNGT 9 cut(s) 477, 607, 1969, 2028, 2536, 2755, 2843, 2903, 3855
Bst6I CTCTTC 4 cut(s) 92, 3864, 4496, 4684
BstACI GRCGYC 1 cut(s) 4533
BstAPI GCANNNNNTGC 1 cut(s) 3022
BstAUI TGTACA 1 cut(s) 4168
BstBAI YACGTR 4 cut(s) 1775, 2918, 4095, 4193
BstBI TTCGAA 1 cut(s) 3514
BstDSI CCRYGG 2 cut(s) 1944, 3387
BstENI CCTNNNNNAGG 1 cut(s) 1353
BstF5I GGATG 8 cut(s) 904, 1909, 1926, 2980, 3130, 3872, 3992, 4777
BstFNI CGCG 3 cut(s) 327, 329, 3334
BstHHI GCGC 3 cut(s) 329, 1951, 3334
BstMAI GTCTC 6 cut(s) 421, 613, 1852, 2400, 3861, 4657
BstNI CCWGG 7 cut(s) 452, 1368, 2277, 2339, 3292, 3498, 3807
BstNSI RCATGY 5 cut(s) 487, 1280, 1865, 3048, 4131
BstSFI CTRYAG 6 cut(s) 366, 423, 654, 2024, 3364, 4695
BstSLI GKGCMC 3 cut(s) 365, 2886, 3220
BstSNI TACGTA 1 cut(s) 1775
BstUI CGCG 3 cut(s) 327, 329, 3334
BstV2I GAAGAC 2 cut(s) 522, 2139
BstX2I RGATCY 8 cut(s) 857, 1023, 1683, 1717, 2868, 3802, 4276, 4500
BstXI CCANNNNNNTGG 3 cut(s) 3497, 4130, 4368
BstYI RGATCY 8 cut(s) 857, 1023, 1683, 1717, 2868, 3802, 4276, 4500
BstZ17I GTATAC 1 cut(s) 1739
Bsu36I CCTNAGG 2 cut(s) 2940, 4021
BsuI GTATCC 1 cut(s) 3315
BtgI CCRYGG 2 cut(s) 1944, 3387
BtsCI GGATG 8 cut(s) 904, 1909, 1926, 2980, 3130, 3872, 3992, 4777
BtsI GCAGTG 3 cut(s) 967, 1152, 2459
BtsIMutI CAGTG 8 cut(s) 967, 1152, 2459, 2751, 2908, 3226, 4218, 4416
BveI ACCTGC 2 cut(s) 2143, 2181
CaiI CAGNNNCTG 1 cut(s) 2779
CfoI GCGC 3 cut(s) 329, 1951, 3334
Cfr10I RCCGGY 1 cut(s) 397
Csp6I GTAC 8 cut(s) 1187, 1287, 2159, 2335, 3251, 3407, 3527, 4169
CspCI CAANNNNNGTGG 2 cut(s) 4297, 4332
CviQI GTAC 8 cut(s) 1187, 1287, 2159, 2335, 3251, 3407, 3527, 4169
DraI TTTAAA 3 cut(s) 750, 2112, 3702
DrdI GACNNNNNNGTC 1 cut(s) 1472
DseDI GACNNNNNNGTC 1 cut(s) 1472
EaeI YGGCCR 3 cut(s) 580, 3411, 3487
Eam1104I CTCTTC 4 cut(s) 92, 3864, 4496, 4684
EarI CTCTTC 4 cut(s) 92, 3864, 4496, 4684
Eco105I TACGTA 1 cut(s) 1775
Eco130I CCWWGG 3 cut(s) 1944, 3274, 3387
Eco31I GGTCTC 2 cut(s) 2400, 4657
Eco32I GATATC 1 cut(s) 3535
Eco57I CTGAAG 5 cut(s) 18, 1451, 1811, 2990, 3984
Eco72I CACGTG 1 cut(s) 2918
Eco81I CCTNAGG 2 cut(s) 2940, 4021
Eco88I CYCGRG 2 cut(s) 42, 140
EcoNI CCTNNNNNAGG 1 cut(s) 1353
EcoO109I RGGNCCY 7 cut(s) 1213, 1346, 1358, 3028, 3271, 4380, 4637
EcoRI GAATTC 2 cut(s) 511, 2507
EcoRII CCWGG 7 cut(s) 450, 1366, 2275, 2337, 3290, 3496, 3805
EcoRV GATATC 1 cut(s) 3535
EcoT14I CCWWGG 3 cut(s) 1944, 3274, 3387
ErhI CCWWGG 3 cut(s) 1944, 3274, 3387
FalI AAGNNNNNCTT 4 cut(s) 1455, 1487, 2338, 2370
FaqI GGGAC 5 cut(s) 398, 838, 1111, 1768, 4393
FauI CCCGC 2 cut(s) 1122, 2141
FauNDI CATATG 1 cut(s) 783
FbaI TGATCA 5 cut(s) 250, 1069, 1971, 2584, 2757
FblI GTMKAC 3 cut(s) 1738, 4530, 4705
FokI GGATG 8 cut(s) 911, 1916, 1933, 2987, 3137, 3859, 3979, 4764
GlaI GCGC 3 cut(s) 328, 1950, 3333
GsaI CCCAGC 1 cut(s) 3635
GsuI CTGGAG 4 cut(s) 218, 2013, 2880, 3720
HapII CCGG 8 cut(s) 398, 579, 821, 3240, 3344, 3608, 3863, 4238
HhaI GCGC 3 cut(s) 329, 1951, 3334
Hin1I GRCGYC 1 cut(s) 4533
Hin6I GCGC 3 cut(s) 327, 1949, 3332
HinP1I GCGC 3 cut(s) 327, 1949, 3332
HincII GTYRAC 3 cut(s) 919, 1437, 4531
HindII GTYRAC 3 cut(s) 919, 1437, 4531
HindIII AAGCTT 4 cut(s) 1153, 2352, 3503, 4469
HpaII CCGG 8 cut(s) 398, 579, 821, 3240, 3344, 3608, 3863, 4238
HphI GGTGA 5 cut(s) 1151, 1158, 1290, 2781, 4227
Hpy99I CGWCG 1 cut(s) 4535
HpyCH4III ACNGT 9 cut(s) 477, 607, 1969, 2028, 2536, 2755, 2843, 2903, 3855
HpyCH4IV ACGT 9 cut(s) 703, 1315, 1774, 2401, 2917, 4094, 4192, 4283, 4533
HpySE526I ACGT 9 cut(s) 703, 1315, 1774, 2401, 2917, 4094, 4192, 4283, 4533
Hsp92I GRCGYC 1 cut(s) 4533
HspAI GCGC 3 cut(s) 327, 1949, 3332
KflI GGGWCCC 1 cut(s) 4380
Kpn2I TCCGGA 1 cut(s) 3607
KpnI GGTACC 1 cut(s) 2338
KroI GCCGGC 1 cut(s) 397
KroNI GCCGGC 1 cut(s) 399
Ksp22I TGATCA 5 cut(s) 250, 1069, 1971, 2584, 2757
LguI GCTCTTC 1 cut(s) 4684
LweI GCATC 8 cut(s) 1645, 2569, 2865, 3373, 3685, 3881, 4196, 4732
MaeII ACGT 9 cut(s) 703, 1315, 1774, 2401, 2917, 4094, 4192, 4283, 4533
MfeI CAATTG 4 cut(s) 1407, 1914, 1975, 4103
MflI RGATCY 8 cut(s) 857, 1023, 1683, 1717, 2868, 3802, 4276, 4500
MhlI GDGCHC 5 cut(s) 365, 2886, 3220, 3228, 3721
MlsI TGGCCA 1 cut(s) 3413
MluNI TGGCCA 1 cut(s) 3413
MlyI GAGTC 7 cut(s) 287, 1093, 2129, 2263, 3566, 3727, 4244
MmeI TCCRAC 5 cut(s) 515, 2055, 2525, 4254, 4304
Mox20I TGGCCA 1 cut(s) 3413
MroI TCCGGA 1 cut(s) 3607
MroNI GCCGGC 1 cut(s) 397
MscI TGGCCA 1 cut(s) 3413
MslI CAYNNNNRTG 3 cut(s) 1061, 1264, 2969
Msp20I TGGCCA 1 cut(s) 3413
MspA1I CMGCKG 2 cut(s) 262, 3398
MspI CCGG 8 cut(s) 398, 579, 821, 3240, 3344, 3608, 3863, 4238
MunI CAATTG 4 cut(s) 1407, 1914, 1975, 4103
Mva1269I GAATGC 2 cut(s) 3016, 4552
MvaI CCWGG 7 cut(s) 452, 1368, 2277, 2339, 3292, 3498, 3807
MvnI CGCG 3 cut(s) 327, 329, 3334
NaeI GCCGGC 1 cut(s) 399
NciI CCSGG 5 cut(s) 579, 822, 3240, 3344, 4239
NcoI CCATGG 2 cut(s) 1944, 3387
NdeI CATATG 1 cut(s) 783
NgoMIV GCCGGC 1 cut(s) 397
NmeAIII GCCGAG 2 cut(s) 959, 4686
NspI RCATGY 5 cut(s) 487, 1280, 1865, 3048, 4131
NspV TTCGAA 1 cut(s) 3514
PaeI GCATGC 2 cut(s) 487, 1280
PciSI GCTCTTC 1 cut(s) 4684
PcsI WCGNNNNNNNCGW 1 cut(s) 2923
PctI GAATGC 2 cut(s) 3016, 4552
PdiI GCCGGC 1 cut(s) 399
Pfl23II CGTACG 1 cut(s) 3250
PflFI GACNNNGTC 1 cut(s) 277
PflMI CCANNNNNTGG 2 cut(s) 457, 1205
PfoI TCCNGGA 2 cut(s) 3290, 3496
PleI GAGTC 7 cut(s) 286, 1093, 2129, 2262, 3565, 3727, 4244
PmaCI CACGTG 1 cut(s) 2918
PmlI CACGTG 1 cut(s) 2918
PpsI GAGTC 7 cut(s) 286, 1093, 2129, 2262, 3565, 3727, 4244
Ppu21I YACGTR 4 cut(s) 1775, 2918, 4095, 4193
PpuMI RGGWCCY 5 cut(s) 1213, 1346, 1358, 3028, 4380
PshBI ATTAAT 2 cut(s) 3356, 4118
PsiI TTATAA 2 cut(s) 1502, 1754
Psp5II RGGWCCY 5 cut(s) 1213, 1346, 1358, 3028, 4380
Psp6I CCWGG 7 cut(s) 450, 1366, 2275, 2337, 3290, 3496, 3805
PspCI CACGTG 1 cut(s) 2918
PspFI CCCAGC 1 cut(s) 3631
PspGI CCWGG 7 cut(s) 450, 1366, 2275, 2337, 3290, 3496, 3805
PspLI CGTACG 1 cut(s) 3250
PspPPI RGGWCCY 5 cut(s) 1213, 1346, 1358, 3028, 4380
PstI CTGCAG 3 cut(s) 427, 658, 4699
PstNI CAGNNNCTG 1 cut(s) 2779
PsuI RGATCY 8 cut(s) 857, 1023, 1683, 1717, 2868, 3802, 4276, 4500
PsyI GACNNNGTC 1 cut(s) 277
PvuII CAGCTG 1 cut(s) 262
RsaI GTAC 8 cut(s) 1188, 1288, 2160, 2336, 3252, 3408, 3528, 4170
RsaNI GTAC 8 cut(s) 1187, 1287, 2159, 2335, 3251, 3407, 3527, 4169
RseI CAYNNNNRTG 3 cut(s) 1061, 1264, 2969
SalI GTCGAC 1 cut(s) 4529
SapI GCTCTTC 1 cut(s) 4684
ScaI AGTACT 1 cut(s) 3408
SchI GAGTC 7 cut(s) 287, 1093, 2129, 2263, 3566, 3727, 4244
SduI GDGCHC 5 cut(s) 365, 2886, 3220, 3228, 3721
SfaNI GCATC 8 cut(s) 1645, 2569, 2865, 3373, 3685, 3881, 4196, 4732
SfcI CTRYAG 6 cut(s) 366, 423, 654, 2024, 3364, 4695
SfuI TTCGAA 1 cut(s) 3514
SmiMI CAYNNNNRTG 3 cut(s) 1061, 1264, 2969
SmlI CTYRAG 2 cut(s) 1353, 4293
SmoI CTYRAG 2 cut(s) 1353, 4293
SnaBI TACGTA 1 cut(s) 1775
SpeI ACTAGT 1 cut(s) 1543
SphI GCATGC 2 cut(s) 487, 1280
SspI AATATT 3 cut(s) 2167, 2959, 4324
StyI CCWWGG 3 cut(s) 1944, 3274, 3387
TaaI ACNGT 9 cut(s) 477, 607, 1969, 2028, 2536, 2755, 2843, 2903, 3855
TaiI ACGT 9 cut(s) 706, 1318, 1777, 2404, 2920, 4097, 4195, 4286, 4536
TatI WGTACW 5 cut(s) 1186, 1286, 3406, 3526, 4168
TauI GCSGC 2 cut(s) 74, 1512
TscAI CASTG 8 cut(s) 967, 1152, 2466, 2758, 2908, 3233, 4225, 4416
TspGWI ACGGA 4 cut(s) 2213, 3648, 4032, 4433
TspRI CASTG 8 cut(s) 967, 1152, 2466, 2758, 2908, 3233, 4225, 4416
Tth111I GACNNNGTC 1 cut(s) 277
Van91I CCANNNNNTGG 2 cut(s) 457, 1205
VneI GTGCAC 1 cut(s) 361
VspI ATTAAT 2 cut(s) 3356, 4118
XagI CCTNNNNNAGG 1 cut(s) 1353
XceI RCATGY 5 cut(s) 487, 1280, 1865, 3048, 4131
XcmI CCANNNNNNNNNTGG 2 cut(s) 458, 1302
XmiI GTMKAC 3 cut(s) 1738, 4530, 4705
ZraI GACGTC 1 cut(s) 4534
ZrmI AGTACT 1 cut(s) 3408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.