AT1G25280

Tubby-like F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007029
Physical Location & Seq
Forward (+)
8863768 .. 8866894
3127 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G25280.2

Sequence Viewer

Length: 1338 bp
ATGTCGTTTCGAGGCATTGTTCAAGATTTGAGAGATGGGTTTGGGAGCTTGTCAAGGAGGAGTTTCGATTTTAGGCTCTCGAGTCTTCATAAAGGGAAAGCTCAGGGTTCTTCGTTCCGTGAGTATTCGTCATCCCGTGATCTCTTGTCGCCTGTGATAGTTCAGACAAGTAGATGGGCTAATCTTCCTCCAGAGTTACTCTTTGATGTGATCAAAAGATTAGAGGAAAGTGAGAGTAATTGGCCTGCAAGAAAACATGTTGTGGCTTGTGCTTCGGTTTGTCGGTCTTGGAGAGCTATGTGCCAAGAGATTGTTTTGGGGCCTGAAATCTGTGGGAAACTCACTTTCCCTGTTTCCCTCAAACAGCCAGGGCCTCGTGATGCAATGATTCAGTGTTTCATCAAAAGGGATAAATCAAAGCTAACATTTCACCTTTTTCTTTGTTTAAGTCCCGCTCTATTAGTGGAGAATGGGAAATTTCTTCTTTCAGCTAAAAGAACTCGTAGAACTACTCGAACCGAGTACATTATCTCCATGGATGCTGATAACATCTCAAGATCCAGCAACTCTTACCTCGGAAAGCTCAGATCAAACTTCCTTGGGACAAAGTTCTTGGTGTACGACACGCAACCACCACCAAACACATCTTCGAGCGCACTTATCACTGATCGAACAAGCCGAAGCAGGTTTCACTCCAGACGAGTTTCTCCTAAAGTACCATCCGGAAGCTACAACATTGCTCAAATCACCTATGAGCTCAACGTGTTGGGCACACGCGGGCCACGACGAATGCACTGCATCATGAACTCCATCCCAATTTCATCGCTCGAACCAGGCGGTTCAGTCCCTAACCAACCCGAGAAACTCGTCCCTGCACCATACTCTCTCGACGACTCATTCCGCAGTAACATCTCCTTCTCCAAATCATCATTTGACCACCGCTCCCTCGATTTCAGCAGTTCTAGATTCTCCGAAATGGGAATATCCTGCGACGACAACGAAGAAGAAGCGAGTTTCAGACCGTTGATTCTAAAGAACAAGCAGCCAAGGTGGCACGAGCAGTTGCAATGCTGGTGTTTGAATTTCCGCGGACGTGTGACAGTTGCATCGGTTAAGAATTTCCAGCTTGTAGCAGCAAGACAGCCGCAGCCTCAAGGGACAGGTGCAGCAGCAGCACCAACAAGTGCACCTGCTCACCCTGAGCAAGACAAGGTGATTCTCCAGTTTGGTAAAGTAGGGAAAGATATGTTCACAATGGACTATAGGTATCCATTATCGGCGTTTCAGGCGTTTGCGATATGCTTAAGCAGCTTTGACACCAAGCTTGCTTGTGAATAG

Protein Analysis

445

Amino Acids

50.01

Weight (kDa)

9.61

Isoelectric Point (pI)

66.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tub PF01167 1 - 262 4.1e-83 Tub family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1198
Acc36I ACCTGC 2 cut(s) 675, 1198
AccBSI CCGCTC 2 cut(s) 455, 942
AccII CGCG 2 cut(s) 777, 1089
AccIII TCCGGA 1 cut(s) 722
AciI CCGC 8 cut(s) 453, 777, 837, 901, 940, 1087, 1089, 1145
AclWI GGATC 1 cut(s) 552
AcsI RAATTY 3 cut(s) 476, 1081, 1117
AfaI GTAC 3 cut(s) 524, 620, 717
AflII CTTAAG 1 cut(s) 1303
AflIII ACRYGT 3 cut(s) 256, 762, 1093
AgsI TTSAA 2 cut(s) 23, 1081
AjiI CACGTC 1 cut(s) 1094
AjnI CCWGG 2 cut(s) 367, 832
AjuI GAANNNNNNNTTGG 2 cut(s) 631, 663
Alw21I GWGCWC 2 cut(s) 759, 1189
Alw44I GTGCAC 1 cut(s) 1185
AlwI GGATC 1 cut(s) 552
Ama87I CYCGRG 2 cut(s) 79, 857
Aor13HI TCCGGA 1 cut(s) 722
AoxI GGCC 4 cut(s) 242, 320, 371, 779
ApaLI GTGCAC 1 cut(s) 1185
ApeKI GCWGC 7 cut(s) 1042, 1133, 1147, 1166, 1169, 1172, 1308
ApoI RAATTY 3 cut(s) 476, 1081, 1117
AspLEI GCGC 1 cut(s) 656
AspS9I GGNCC 3 cut(s) 320, 371, 779
AsuHPI GGTGA 4 cut(s) 422, 739, 1187, 1225
AvaI CYCGRG 2 cut(s) 79, 857
BaeGI GKGCMC 2 cut(s) 773, 1189
BanII GRGCYC 1 cut(s) 759
BauI CACGAG 2 cut(s) 375, 1055
BbsI GAAGAC 1 cut(s) 77
Bbv12I GWGCWC 2 cut(s) 759, 1189
BbvI GCAGC 7 cut(s) 1054, 1145, 1159, 1178, 1181, 1184, 1320
BccI CCATC 4 cut(s) 29, 168, 727, 818
BcgI CGANNNNNNTGC 2 cut(s) 777, 811
BciT130I CCWGG 2 cut(s) 369, 834
BciVI GTATCC 1 cut(s) 1278
BclI TGATCA 1 cut(s) 210
BfaI CTAG 1 cut(s) 963
BfmI CTRYAG 1 cut(s) 1261
BfrI CTTAAG 1 cut(s) 1303
BfuAI ACCTGC 2 cut(s) 675, 1198
BfuI GTATCC 1 cut(s) 1278
BglI GCCNNNNNGGC 1 cut(s) 1051
BisI GCNGC 8 cut(s) 1043, 1134, 1145, 1148, 1167, 1170, 1173, 1309
BlsI GCNGC 8 cut(s) 1044, 1135, 1146, 1149, 1168, 1171, 1174, 1310
Bme1390I CCNGG 2 cut(s) 369, 834
BmeT110I CYCGRG 2 cut(s) 79, 857
BmgBI CACGTC 1 cut(s) 1094
BmgT120I GGNCC 3 cut(s) 320, 371, 779
BmiI GGNNCC 1 cut(s) 321
BmrFI CCNGG 2 cut(s) 369, 834
BmsI GCATC 4 cut(s) 370, 529, 807, 1115
BpiI GAAGAC 1 cut(s) 77
BpmI CTGGAG 3 cut(s) 174, 679, 1205
Bpu10I CCTNAGC 2 cut(s) 102, 1200
BpuEI CTTGAG 2 cut(s) 538, 1137
BsaJI CCNNGG 6 cut(s) 368, 534, 574, 598, 1046, 1087
BsaWI WCCGGW 1 cut(s) 722
BsaXI ACNNNNNCTCC 4 cut(s) 515, 545, 926, 956
Bse1I ACTGG 1 cut(s) 1222
Bse3DI GCAATG 3 cut(s) 390, 735, 1073
BseAI TCCGGA 1 cut(s) 722
BseBI CCWGG 2 cut(s) 369, 834
BseDI CCNNGG 6 cut(s) 368, 534, 574, 598, 1046, 1087
BseGI GGATG 4 cut(s) 131, 544, 719, 810
BseMI GCAATG 3 cut(s) 390, 735, 1073
BseMII CTCAG 3 cut(s) 116, 598, 1191
BseNI ACTGG 1 cut(s) 1222
BseRI GAGGAG 1 cut(s) 73
BseSI GKGCMC 2 cut(s) 773, 1189
BseXI GCAGC 7 cut(s) 1054, 1145, 1159, 1178, 1181, 1184, 1320
BsgI GTGCAG 2 cut(s) 858, 1185
Bsh1236I CGCG 2 cut(s) 777, 1089
BshFI GGCC 4 cut(s) 244, 322, 373, 781
BsiHKAI GWGCWC 2 cut(s) 759, 1189
BsiHKCI CYCGRG 2 cut(s) 79, 857
BsiSI CCGG 1 cut(s) 723
BslFI GGGAC 5 cut(s) 435, 616, 830, 854, 1171
BsmFI GGGAC 5 cut(s) 435, 616, 830, 854, 1171
BsmI GAATGC 1 cut(s) 795
BsnI GGCC 4 cut(s) 244, 322, 373, 781
BsoBI CYCGRG 2 cut(s) 79, 857
Bsp1286I GDGCHC 3 cut(s) 759, 773, 1189
Bsp13I TCCGGA 1 cut(s) 722
Bsp143I GATC 5 cut(s) 139, 210, 557, 587, 667
Bsp19I CCATGG 1 cut(s) 534
BspACI CCGC 8 cut(s) 453, 777, 837, 901, 940, 1087, 1089, 1145
BspANI GGCC 4 cut(s) 244, 322, 373, 781
BspCNI CTCAG 3 cut(s) 115, 597, 1192
BspEI TCCGGA 1 cut(s) 722
BspFNI CGCG 2 cut(s) 777, 1089
BspHI TCATGA 1 cut(s) 801
BspLI GGNNCC 1 cut(s) 321
BspMI ACCTGC 2 cut(s) 675, 1198
BspPI GGATC 1 cut(s) 552
BspTI CTTAAG 1 cut(s) 1303
BsrBI CCGCTC 2 cut(s) 455, 942
BsrDI GCAATG 3 cut(s) 390, 735, 1073
BsrI ACTGG 1 cut(s) 1222
BssECI CCNNGG 6 cut(s) 368, 534, 574, 598, 1046, 1087
BssMI GATC 5 cut(s) 139, 210, 557, 587, 667
BssSI CACGAG 2 cut(s) 375, 1055
BssT1I CCWWGG 3 cut(s) 534, 598, 1046
Bst2BI CACGAG 2 cut(s) 375, 1055
Bst2UI CCWGG 2 cut(s) 369, 834
Bst4CI ACNGT 2 cut(s) 1023, 1102
BstAFI CTTAAG 1 cut(s) 1303
BstC8I GCNNGC 3 cut(s) 246, 779, 1326
BstDEI CTNAG 3 cut(s) 102, 584, 1200
BstDSI CCRYGG 2 cut(s) 534, 1087
BstF5I GGATG 4 cut(s) 131, 544, 719, 810
BstFNI CGCG 2 cut(s) 777, 1089
BstHHI GCGC 1 cut(s) 656
BstKTI GATC 5 cut(s) 142, 213, 560, 590, 670
BstMBI GATC 5 cut(s) 139, 210, 557, 587, 667
BstMWI GCNNNNNNNGC 4 cut(s) 1051, 1172, 1286, 1308
BstNI CCWGG 2 cut(s) 369, 834
BstNSI RCATGY 1 cut(s) 260
BstSCI CCNGG 2 cut(s) 367, 832
BstSFI CTRYAG 1 cut(s) 1261
BstSLI GKGCMC 2 cut(s) 773, 1189
BstUI CGCG 2 cut(s) 777, 1089
BstV1I GCAGC 7 cut(s) 1054, 1145, 1159, 1178, 1181, 1184, 1320
BstV2I GAAGAC 1 cut(s) 77
BstX2I RGATCY 1 cut(s) 557
BstYI RGATCY 1 cut(s) 557
BsuI GTATCC 1 cut(s) 1278
BsuRI GGCC 4 cut(s) 244, 322, 373, 781
BtgI CCRYGG 2 cut(s) 534, 1087
BtgZI GCGATG 1 cut(s) 807
BtrI CACGTC 1 cut(s) 1094
BtsCI GGATG 4 cut(s) 131, 544, 719, 810
BtsI GCAGTG 1 cut(s) 793
BtsIMutI CAGTG 3 cut(s) 398, 663, 793
BveI ACCTGC 2 cut(s) 675, 1198
Cac8I GCNNGC 3 cut(s) 246, 779, 1326
CciI TCATGA 1 cut(s) 801
CfoI GCGC 1 cut(s) 656
Cfr13I GGNCC 3 cut(s) 320, 371, 779
Cfr42I CCGCGG 1 cut(s) 1090
Csp6I GTAC 3 cut(s) 523, 619, 716
CviAII CATG 3 cut(s) 257, 535, 802
CviQI GTAC 3 cut(s) 523, 619, 716
DdeI CTNAG 3 cut(s) 102, 584, 1200
DpnI GATC 5 cut(s) 141, 212, 559, 589, 669
DpnII GATC 5 cut(s) 139, 210, 557, 587, 667
Ecl136II GAGCTC 1 cut(s) 757
Eco130I CCWWGG 3 cut(s) 534, 598, 1046
Eco24I GRGCYC 1 cut(s) 759
Eco53kI GAGCTC 1 cut(s) 757
Eco88I CYCGRG 2 cut(s) 79, 857
EcoICRI GAGCTC 1 cut(s) 757
EcoO109I RGGNCCY 2 cut(s) 320, 371
EcoRII CCWGG 2 cut(s) 367, 832
EcoT14I CCWWGG 3 cut(s) 534, 598, 1046
EcoT38I GRGCYC 1 cut(s) 759
ErhI CCWWGG 3 cut(s) 534, 598, 1046
FaeI CATG 3 cut(s) 260, 538, 805
FaqI GGGAC 5 cut(s) 435, 616, 830, 854, 1171
FatI CATG 3 cut(s) 256, 534, 801
FauI CCCGC 2 cut(s) 460, 770
FbaI TGATCA 1 cut(s) 210
Fnu4HI GCNGC 8 cut(s) 1043, 1134, 1145, 1148, 1167, 1170, 1173, 1309
FokI GGATG 4 cut(s) 118, 551, 706, 797
FriOI GRGCYC 1 cut(s) 759
Fsp4HI GCNGC 8 cut(s) 1043, 1134, 1145, 1148, 1167, 1170, 1173, 1309
FspBI CTAG 1 cut(s) 963
GlaI GCGC 1 cut(s) 655
GluI GCNGC 8 cut(s) 1043, 1134, 1145, 1148, 1167, 1170, 1173, 1309
GsuI CTGGAG 3 cut(s) 174, 679, 1205
HaeIII GGCC 4 cut(s) 244, 322, 373, 781
HapII CCGG 1 cut(s) 723
HhaI GCGC 1 cut(s) 656
Hin1II CATG 3 cut(s) 260, 538, 805
Hin6I GCGC 1 cut(s) 654
HinP1I GCGC 1 cut(s) 654
HindIII AAGCTT 1 cut(s) 1322
HinfI GANTC 6 cut(s) 82, 388, 893, 966, 1027, 1216
HpaII CCGG 1 cut(s) 723
HphI GGTGA 4 cut(s) 422, 739, 1187, 1225
Hpy166II GTNNAC 3 cut(s) 619, 1187, 1251
Hpy188I TCNGA 5 cut(s) 165, 578, 587, 973, 1019
Hpy8I GTNNAC 3 cut(s) 619, 1187, 1251
Hpy99I CGWCG 3 cut(s) 789, 893, 995
HpyAV CCTTC 1 cut(s) 925
HpyCH4III ACNGT 2 cut(s) 1023, 1102
HpyCH4IV ACGT 2 cut(s) 762, 1093
HpyCH4V TGCA 9 cut(s) 248, 383, 793, 798, 875, 1066, 1106, 1166, 1187
HpyF10VI GCNNNNNNNGC 4 cut(s) 1051, 1172, 1286, 1308
HpyF3I CTNAG 3 cut(s) 102, 584, 1200
HpySE526I ACGT 2 cut(s) 762, 1093
Hsp92II CATG 3 cut(s) 260, 538, 805
HspAI GCGC 1 cut(s) 654
Kpn2I TCCGGA 1 cut(s) 722
Ksp22I TGATCA 1 cut(s) 210
KspI CCGCGG 1 cut(s) 1090
Kzo9I GATC 5 cut(s) 139, 210, 557, 587, 667
LmnI GCTCC 2 cut(s) 45, 947
Lsp1109I GCAGC 7 cut(s) 1054, 1145, 1159, 1178, 1181, 1184, 1320
LweI GCATC 4 cut(s) 370, 529, 807, 1115
MaeI CTAG 1 cut(s) 963
MaeII ACGT 2 cut(s) 762, 1093
MaeIII GTNAC 3 cut(s) 195, 905, 1096
MalI GATC 5 cut(s) 141, 212, 559, 589, 669
MbiI CCGCTC 2 cut(s) 455, 942
MboI GATC 5 cut(s) 139, 210, 557, 587, 667
MboII GAAGA 7 cut(s) 77, 102, 176, 473, 639, 1013, 1016
MflI RGATCY 1 cut(s) 557
MhlI GDGCHC 3 cut(s) 759, 773, 1189
MluCI AATT 5 cut(s) 238, 476, 816, 1081, 1117
MlyI GAGTC 2 cut(s) 91, 887
MnlI CCTC 9 cut(s) 5, 51, 198, 217, 368, 384, 584, 956, 1161
MroI TCCGGA 1 cut(s) 722
MseI TTAA 3 cut(s) 446, 1113, 1304
MspA1I CMGCKG 1 cut(s) 1089
MspCI CTTAAG 1 cut(s) 1303
MspI CCGG 1 cut(s) 723
MspR9I CCNGG 2 cut(s) 369, 834
Mva1269I GAATGC 1 cut(s) 795
MvaI CCWGG 2 cut(s) 369, 834
MvnI CGCG 2 cut(s) 777, 1089
MwoI GCNNNNNNNGC 4 cut(s) 1051, 1172, 1286, 1308
NcoI CCATGG 1 cut(s) 534
NdeII GATC 5 cut(s) 139, 210, 557, 587, 667
NlaIII CATG 3 cut(s) 260, 538, 805
NlaIV GGNNCC 1 cut(s) 321
NmuCI GTSAC 1 cut(s) 1096
NspI RCATGY 1 cut(s) 260
PaeR7I CTCGAG 1 cut(s) 79
PagI TCATGA 1 cut(s) 801
PaqCI CACCTGC 1 cut(s) 1198
PciI ACATGT 1 cut(s) 256
PcsI WCGNNNNNNNCGW 2 cut(s) 676, 781
PctI GAATGC 1 cut(s) 795
PfeI GAWTC 4 cut(s) 388, 966, 1027, 1216
PkrI GCNGC 8 cut(s) 1044, 1135, 1146, 1149, 1168, 1171, 1174, 1310
PleI GAGTC 2 cut(s) 90, 887
PpsI GAGTC 2 cut(s) 90, 887
PscI ACATGT 1 cut(s) 256
Psp124BI GAGCTC 1 cut(s) 759
Psp6I CCWGG 2 cut(s) 367, 832
PspGI CCWGG 2 cut(s) 367, 832
PspN4I GGNNCC 1 cut(s) 321
PspPI GGNCC 3 cut(s) 320, 371, 779
PsuI RGATCY 1 cut(s) 557
RsaI GTAC 3 cut(s) 524, 620, 717
RsaNI GTAC 3 cut(s) 523, 619, 716
SacI GAGCTC 1 cut(s) 759
SacII CCGCGG 1 cut(s) 1090
SaqAI TTAA 3 cut(s) 446, 1113, 1304
SatI GCNGC 8 cut(s) 1043, 1134, 1145, 1148, 1167, 1170, 1173, 1309
Sau3AI GATC 5 cut(s) 139, 210, 557, 587, 667
Sau96I GGNCC 3 cut(s) 320, 371, 779
SchI GAGTC 2 cut(s) 91, 887
ScrFI CCNGG 2 cut(s) 369, 834
SduI GDGCHC 3 cut(s) 759, 773, 1189
SfaNI GCATC 4 cut(s) 370, 529, 807, 1115
SfcI CTRYAG 1 cut(s) 1261
Sfr274I CTCGAG 1 cut(s) 79
Sfr303I CCGCGG 1 cut(s) 1090
SgrBI CCGCGG 1 cut(s) 1090
SlaI CTCGAG 1 cut(s) 79
SmlI CTYRAG 4 cut(s) 79, 553, 1152, 1303
SmoI CTYRAG 4 cut(s) 79, 553, 1152, 1303
Sse9I AATT 5 cut(s) 238, 476, 816, 1081, 1117
SsiI CCGC 8 cut(s) 453, 777, 837, 901, 940, 1087, 1089, 1145
SspMI CTAG 1 cut(s) 963
SstI GAGCTC 1 cut(s) 759
StyD4I CCNGG 2 cut(s) 367, 832
StyI CCWWGG 3 cut(s) 534, 598, 1046
TaaI ACNGT 2 cut(s) 1023, 1102
TaiI ACGT 2 cut(s) 765, 1096
TaqI TCGA 9 cut(s) 10, 66, 80, 514, 650, 670, 828, 888, 948
TaqII GACCGA 1 cut(s) 273
TasI AATT 5 cut(s) 238, 476, 816, 1081, 1117
TatI WGTACW 1 cut(s) 522
TauI GCSGC 1 cut(s) 1147
TfiI GAWTC 4 cut(s) 388, 966, 1027, 1216
Tru1I TTAA 3 cut(s) 446, 1113, 1304
Tru9I TTAA 3 cut(s) 446, 1113, 1304
TscAI CASTG 3 cut(s) 398, 670, 800
TseFI GTSAC 1 cut(s) 1096
TseI GCWGC 7 cut(s) 1042, 1133, 1147, 1166, 1169, 1172, 1308
Tsp45I GTSAC 1 cut(s) 1096
TspDTI ATGAA 4 cut(s) 77, 388, 810, 818
TspGWI ACGGA 1 cut(s) 107
TspRI CASTG 3 cut(s) 398, 670, 800
Vha464I CTTAAG 1 cut(s) 1303
VneI GTGCAC 1 cut(s) 1185
XapI RAATTY 3 cut(s) 476, 1081, 1117
XbaI TCTAGA 1 cut(s) 962
XceI RCATGY 1 cut(s) 260
XhoI CTCGAG 1 cut(s) 79
XspI CTAG 1 cut(s) 963
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.