Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0007029

Physical Location & Seq

Genomic Coordinates Forward (+)

28953614 .. 28959910

Gene Structure 6297 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1224600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1275 bp

ATGTCGTTCCGTAGCATAGTTCGTGATGTTAGGGATGGCTTTGGGAGCTTATCTAGACGGGGTTTTGAAGTCCGGCTGACGAGCCATCATAGAGGAAAATCTCATGGCTCGTTAAATGATTTACATGACCAGCCTCTCATTGTTCAGAACAGTCGTTGGGCTAGCCTCCCCGCAGAACTATTATATGATGTAATCAGGAGGCTGGAGGAGAGTGAGAGCACATGGCCTTCTCGAAAGCATGTTGTTGCATGTGCCTCAGTTTGCCGTTCTTGGAGGATTATGTGCAAAGAAATCGTTAAGAAGCCTGAGATCTGCGGGAAGCTTACTTTTCCTGTGTCGCTGAAGCAGCCAGGTTCACGGGATGGACTCGTTCAATGCTTCATTAAAAGGGATAAATCCAATTTAACATATCATCTATTTCTGTGTCTTAGCCCTGCTTTGCTAGTTGAGAATGGGAAATTCCTTCTTTCTGCAAAGAGGACTCGAAGAACTACTTGTACAGAATATGTTATCTCTATGGATGCTGACAACATCTCTAGATCAAGCAGCAGTTACATTGGAAAGTTAAGGTCAAACTTTCTTGGCACAAAATTCATTGTATACGATACGCAGCCTGCATACACTTCTGAACATGTTCCCCCACCAGGGCGGTCAAGCCGTAGATTTAATTCCAAAAAAGTCTCACCAAAAGTCCCGACTGGCAGCTATAACATTGCTCAGATAACATATGAGCTAAATGTGCTGGGCACTCGCGGTCCTAGAAGGATGCATTGCATCATGCATTCTATCCCGGTCTCATCTCTTGATGCAGGAGGTACTGTCCCTGGCCAACCAGAACTCCTTACCCGAGCCCTGGAAGACTCATTCCGAAGTATCTCTTTCTCAAAGTCTCTTGACCACTCAGTAGAATTCAGCAGTACACGATTTTCTGAAATTGATGTACCCCGAGATGATGAGGATGGCAAGACAAGACCCTTGGTTTTGAAAAACAAGCCCCCACGATGGCATGAACAATTACAGTGTTGGTGCCTGAACTTCCGAGGCAGGGTAACTGTTGCTTCTGTTAAGAACTTTCAGTTGATTGCTGCTACTCAGCCTGCTGCTGGTGCACCTACTCCATCACAGCCAGCGCCGCCAGAGCTCGACAAGATACTCTTGCAGTTTGGCAAGGTTGGTAAAGATATGTTCACGATGGATTATCGCTATCCCTTATCTGCATTTCAGGCTTTTGCTATCTGCTTGAGCAGCTTCGACACGAAATTGGCTTGTGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006355 GO:0008150 GO:0008289 GO:0009536 GO:0009605 GO:0009607 GO:0009620 GO:0009889 GO:0010468 GO:0010556 GO:0016020 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0035091 GO:0043167 GO:0043168 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0071944 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box Tub

Protein Analysis

425

Amino Acids

47.69

Weight (kDa)

9.57

Isoelectric Point (pI)

60.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tub	PF01167	114 - 419	1.5e-107	Tub family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007029)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G25280 AT1G25280 AT1G25280
fragaria_vesca	FvH4_5g30230 FvH4_5g30230 FvH4_5g30230 FvH4_5g30230
malus_domestica	MD08G1224600.v1.1 MD15G1417700.v1.1
prunus_persica	Prupe.1G561700_v2.0.a1 Prupe.1G561700_v2.0.a1 Prupe.1G561700_v2.0.a1
pyrus_communis	pycom08g19550 pycom15g37090
rosa_chinensis	RchiOBHm_Chr7g0224501
rosa_laevigata	RLG00000001890
rosa_multiflora	Rmu_sc0008421.1_g000006
rosa_roxburghii	Rroxscaffold_3G00234680
rosa_rugosa	Rorug07G0223800 Rorug07G0223800
rosa_samantha	Rh7AG379500 Rh7BG359900 Rh7CG398900 Rh7DG369400
rosa_wichuraiana	Rw7G031210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1026
AccI	GTMKAC	1 cut(s)	600
AccII	CGCG	1 cut(s)	753
AciI	CCGC	5 cut(s)	171, 315, 649, 753, 1133
AcoI	YGGCCR	1 cut(s)	826
AcsI	RAATTY	3 cut(s)	458, 590, 908
AcuI	CTGAAG	1 cut(s)	362
AfaI	GTAC	4 cut(s)	499, 817, 919, 942
AfiI	CCNNNNNNNGG	6 cut(s)	644, 645, 853, 1002, 1045, 1103
AflIII	ACRYGT	1 cut(s)	631
AgsI	TTSAA	3 cut(s)	68, 374, 985
AjnI	CCWGG	4 cut(s)	349, 643, 823, 852
AluBI	AGCT	6 cut(s)	48, 322, 705, 733, 1141, 1248
AluI	AGCT	6 cut(s)	48, 322, 705, 733, 1141, 1248
Alw21I	GWGCWC	3 cut(s)	221, 1111, 1143
Alw26I	GTCTC	3 cut(s)	685, 799, 894
Alw44I	GTGCAC	1 cut(s)	1107
Ama87I	CYCGRG	2 cut(s)	846, 945
AoxI	GGCC	2 cut(s)	224, 826
ApaLI	GTGCAC	1 cut(s)	1107
ApeKI	GCWGC	7 cut(s)	346, 546, 610, 702, 1085, 1100, 1245
ApoI	RAATTY	3 cut(s)	458, 590, 908
Asp700I	GAANNNNTTC	1 cut(s)	633
AspLEI	GCGC	1 cut(s)	1132
AspS9I	GGNCC	1 cut(s)	755
AsuC2I	CCSGG	1 cut(s)	791
AsuHPI	GGTGA	1 cut(s)	675
AsuNHI	GCTAGC	1 cut(s)	161
AvaI	CYCGRG	2 cut(s)	846, 945
AvaII	GGWCC	1 cut(s)	755
BaeGI	GKGCMC	2 cut(s)	749, 1111
BalI	TGGCCA	1 cut(s)	828
BanI	GGYRCC	1 cut(s)	1026
BanII	GRGCYC	2 cut(s)	853, 1143
BbsI	GAAGAC	1 cut(s)	864
Bbv12I	GWGCWC	3 cut(s)	221, 1111, 1143
BbvI	GCAGC	7 cut(s)	358, 558, 622, 714, 1072, 1087, 1257
BccI	CCATC	7 cut(s)	29, 93, 356, 953, 996, 1126, 1186
BceAI	ACGGC	2 cut(s)	249, 642
BcgI	CGANNNNNNTGC	2 cut(s)	274, 308
BciT130I	CCWGG	4 cut(s)	351, 645, 825, 854
BcnI	CCSGG	1 cut(s)	791
BcoDI	GTCTC	3 cut(s)	685, 799, 894
BfaI	CTAG	5 cut(s)	54, 162, 443, 537, 759
BfoI	RGCGCY	1 cut(s)	1133
BglII	AGATCT	1 cut(s)	309
BisI	GCNGC	8 cut(s)	347, 547, 611, 703, 1086, 1101, 1133, 1246
BlsI	GCNGC	8 cut(s)	348, 548, 612, 704, 1087, 1102, 1134, 1247
Bme1390I	CCNGG	5 cut(s)	351, 645, 791, 825, 854
Bme18I	GGWCC	1 cut(s)	755
BmeT110I	CYCGRG	2 cut(s)	846, 945
BmgT120I	GGNCC	1 cut(s)	755
BmiI	GGNNCC	1 cut(s)	1028
BmrFI	CCNGG	5 cut(s)	351, 645, 791, 825, 854
BmsI	GCATC	4 cut(s)	511, 756, 783, 796
BmtI	GCTAGC	1 cut(s)	165
BpiI	GAAGAC	1 cut(s)	864
BpmI	CTGGAG	1 cut(s)	224
BpuEI	CTTGAG	1 cut(s)	1261
BpuMI	CCSGG	1 cut(s)	791
BsaI	GGTCTC	1 cut(s)	799
BsaJI	CCNNGG	5 cut(s)	644, 823, 852, 975, 1039
BsaXI	ACNNNNNCTCC	4 cut(s)	804, 822, 834, 852
Bsc4I	CCNNNNNNNGG	6 cut(s)	644, 645, 853, 1002, 1045, 1103
Bse1I	ACTGG	1 cut(s)	703
Bse3DI	GCAATG	2 cut(s)	711, 769
BseBI	CCWGG	4 cut(s)	351, 645, 825, 854
BseDI	CCNNGG	5 cut(s)	644, 823, 852, 975, 1039
BseGI	GGATG	5 cut(s)	40, 367, 526, 771, 964
BseLI	CCNNNNNNNGG	6 cut(s)	644, 645, 853, 1002, 1045, 1103
BseMI	GCAATG	2 cut(s)	711, 769
BseMII	CTCAG	5 cut(s)	270, 297, 731, 915, 1106
BseNI	ACTGG	1 cut(s)	703
BseRI	GAGGAG	1 cut(s)	221
BseSI	GKGCMC	2 cut(s)	749, 1111
BseXI	GCAGC	7 cut(s)	358, 558, 622, 714, 1072, 1087, 1257
BseYI	CCCAGC	1 cut(s)	742
Bsh1236I	CGCG	1 cut(s)	753
BshFI	GGCC	2 cut(s)	226, 828
BshNI	GGYRCC	1 cut(s)	1026
BsiHKAI	GWGCWC	3 cut(s)	221, 1111, 1143
BsiHKCI	CYCGRG	2 cut(s)	846, 945
BsiSI	CCGG	2 cut(s)	73, 791
BslFI	GGGAC	2 cut(s)	677, 806
BslI	CCNNNNNNNGG	6 cut(s)	644, 645, 853, 1002, 1045, 1103
BsmAI	GTCTC	3 cut(s)	685, 799, 894
BsmFI	GGGAC	2 cut(s)	677, 806
BsmI	GAATGC	1 cut(s)	781
BsnI	GGCC	2 cut(s)	226, 828
Bso31I	GGTCTC	1 cut(s)	799
BsoBI	CYCGRG	2 cut(s)	846, 945
Bsp1286I	GDGCHC	5 cut(s)	221, 749, 853, 1111, 1143
Bsp1407I	TGTACA	1 cut(s)	497
Bsp143I	GATC	2 cut(s)	309, 539
BspACI	CCGC	5 cut(s)	171, 315, 649, 753, 1133
BspANI	GGCC	2 cut(s)	226, 828
BspCNI	CTCAG	5 cut(s)	269, 298, 730, 914, 1105
BspFNI	CGCG	1 cut(s)	753
BspLI	GGNNCC	1 cut(s)	1028
BspOI	GCTAGC	1 cut(s)	165
BspT107I	GGYRCC	1 cut(s)	1026
BspTNI	GGTCTC	1 cut(s)	799
BsrDI	GCAATG	2 cut(s)	711, 769
BsrGI	TGTACA	1 cut(s)	497
BsrI	ACTGG	1 cut(s)	703
BssECI	CCNNGG	5 cut(s)	644, 823, 852, 975, 1039
BssMI	GATC	2 cut(s)	309, 539
BssNAI	GTATAC	1 cut(s)	601
BssT1I	CCWWGG	1 cut(s)	975
Bst1107I	GTATAC	1 cut(s)	601
Bst2UI	CCWGG	4 cut(s)	351, 645, 825, 854
Bst4CI	ACNGT	4 cut(s)	152, 820, 1020, 1054
BstAUI	TGTACA	1 cut(s)	497
BstC8I	GCNNGC	4 cut(s)	163, 615, 1098, 1128
BstDEI	CTNAG	6 cut(s)	256, 306, 428, 717, 901, 1092
BstF5I	GGATG	5 cut(s)	40, 367, 526, 771, 964
BstFNI	CGCG	1 cut(s)	753
BstH2I	RGCGCY	1 cut(s)	1133
BstHHI	GCGC	1 cut(s)	1132
BstKTI	GATC	2 cut(s)	312, 542
BstMAI	GTCTC	3 cut(s)	685, 799, 894
BstMBI	GATC	2 cut(s)	309, 539
BstMWI	GCNNNNNNNGC	8 cut(s)	45, 346, 739, 1106, 1132, 1138, 1223, 1245
BstNI	CCWGG	4 cut(s)	351, 645, 825, 854
BstNSI	RCATGY	3 cut(s)	242, 252, 635
BstSCI	CCNGG	5 cut(s)	349, 643, 789, 823, 852
BstSLI	GKGCMC	2 cut(s)	749, 1111
BstUI	CGCG	1 cut(s)	753
BstV1I	GCAGC	7 cut(s)	358, 558, 622, 714, 1072, 1087, 1257
BstV2I	GAAGAC	1 cut(s)	864
BstX2I	RGATCY	1 cut(s)	309
BstYI	RGATCY	1 cut(s)	309
BstZ17I	GTATAC	1 cut(s)	601
BsuRI	GGCC	2 cut(s)	226, 828
BtsCI	GGATG	5 cut(s)	40, 367, 526, 771, 964
BtsIMutI	CAGTG	1 cut(s)	1025
Cac8I	GCNNGC	4 cut(s)	163, 615, 1098, 1128
CfoI	GCGC	1 cut(s)	1132
Cfr13I	GGNCC	1 cut(s)	755
Csp6I	GTAC	4 cut(s)	498, 816, 918, 941
CviAII	CATG	8 cut(s)	104, 125, 222, 239, 249, 632, 778, 1007
CviQI	GTAC	4 cut(s)	498, 816, 918, 941
DdeI	CTNAG	6 cut(s)	256, 306, 428, 717, 901, 1092
DpnI	GATC	2 cut(s)	311, 541
DpnII	GATC	2 cut(s)	309, 539
EaeI	YGGCCR	1 cut(s)	826
Ecl136II	GAGCTC	1 cut(s)	1141
Eco130I	CCWWGG	1 cut(s)	975
Eco24I	GRGCYC	2 cut(s)	853, 1143
Eco31I	GGTCTC	1 cut(s)	799
Eco47I	GGWCC	1 cut(s)	755
Eco53kI	GAGCTC	1 cut(s)	1141
Eco57I	CTGAAG	1 cut(s)	362
Eco88I	CYCGRG	2 cut(s)	846, 945
EcoICRI	GAGCTC	1 cut(s)	1141
EcoRI	GAATTC	1 cut(s)	908
EcoRII	CCWGG	4 cut(s)	349, 643, 823, 852
EcoT14I	CCWWGG	1 cut(s)	975
EcoT22I	ATGCAT	2 cut(s)	771, 783
EcoT38I	GRGCYC	2 cut(s)	853, 1143
ErhI	CCWWGG	1 cut(s)	975
FaeI	CATG	8 cut(s)	107, 128, 225, 242, 252, 635, 781, 1010
FalI	AAGNNNNNCTT	6 cut(s)	478, 510, 862, 894, 1139, 1171
FaqI	GGGAC	2 cut(s)	677, 806
FatI	CATG	8 cut(s)	103, 124, 221, 238, 248, 631, 777, 1006
FauI	CCCGC	2 cut(s)	178, 308
FauNDI	CATATG	1 cut(s)	727
FblI	GTMKAC	1 cut(s)	600
Fnu4HI	GCNGC	8 cut(s)	347, 547, 611, 703, 1086, 1101, 1133, 1246
FokI	GGATG	5 cut(s)	47, 374, 533, 778, 971
FriOI	GRGCYC	2 cut(s)	853, 1143
Fsp4HI	GCNGC	8 cut(s)	347, 547, 611, 703, 1086, 1101, 1133, 1246
FspBI	CTAG	5 cut(s)	54, 162, 443, 537, 759
GlaI	GCGC	1 cut(s)	1131
GluI	GCNGC	8 cut(s)	347, 547, 611, 703, 1086, 1101, 1133, 1246
GsaI	CCCAGC	1 cut(s)	746
GsuI	CTGGAG	1 cut(s)	224
HaeII	RGCGCY	1 cut(s)	1133
HaeIII	GGCC	2 cut(s)	226, 828
HapII	CCGG	2 cut(s)	73, 791
HhaI	GCGC	1 cut(s)	1132
Hin1II	CATG	8 cut(s)	107, 128, 225, 242, 252, 635, 781, 1010
Hin6I	GCGC	1 cut(s)	1130
HinP1I	GCGC	1 cut(s)	1130
HindIII	AAGCTT	1 cut(s)	320
HinfI	GANTC	3 cut(s)	366, 481, 860
HpaII	CCGG	2 cut(s)	73, 791
HphI	GGTGA	1 cut(s)	675
Hpy166II	GTNNAC	5 cut(s)	356, 601, 920, 1109, 1188
Hpy188I	TCNGA	6 cut(s)	147, 628, 720, 869, 931, 1040
Hpy188III	TCNNGA	9 cut(s)	23, 54, 196, 231, 537, 694, 803, 893, 1189
Hpy8I	GTNNAC	5 cut(s)	356, 601, 920, 1109, 1188
HpyAV	CCTTC	3 cut(s)	237, 473, 756
HpyCH4III	ACNGT	4 cut(s)	152, 820, 1020, 1054
HpyF10VI	GCNNNNNNNGC	8 cut(s)	45, 346, 739, 1106, 1132, 1138, 1223, 1245
HpyF3I	CTNAG	6 cut(s)	256, 306, 428, 717, 901, 1092
Hsp92II	CATG	8 cut(s)	107, 128, 225, 242, 252, 635, 781, 1010
HspAI	GCGC	1 cut(s)	1130
Kzo9I	GATC	2 cut(s)	309, 539
LmnI	GCTCC	1 cut(s)	45
Lsp1109I	GCAGC	7 cut(s)	358, 558, 622, 714, 1072, 1087, 1257
LweI	GCATC	4 cut(s)	511, 756, 783, 796
MaeI	CTAG	5 cut(s)	54, 162, 443, 537, 759
MaeIII	GTNAC	2 cut(s)	551, 1048
MalI	GATC	2 cut(s)	311, 541
MboI	GATC	2 cut(s)	309, 539
MboII	GAAGA	2 cut(s)	498, 869
MflI	RGATCY	1 cut(s)	309
MhlI	GDGCHC	5 cut(s)	221, 749, 853, 1111, 1143
MlsI	TGGCCA	1 cut(s)	828
MluCI	AATT	8 cut(s)	400, 458, 590, 667, 908, 933, 1013, 1259
MluNI	TGGCCA	1 cut(s)	828
MlyI	GAGTC	3 cut(s)	360, 475, 854
Mox20I	TGGCCA	1 cut(s)	828
Mph1103I	ATGCAT	2 cut(s)	771, 783
MroXI	GAANNNNTTC	1 cut(s)	633
MscI	TGGCCA	1 cut(s)	828
MseI	TTAA	7 cut(s)	113, 297, 384, 404, 566, 666, 1065
Msp20I	TGGCCA	1 cut(s)	828
MspI	CCGG	2 cut(s)	73, 791
MspR9I	CCNGG	5 cut(s)	351, 645, 791, 825, 854
Mva1269I	GAATGC	1 cut(s)	781
MvaI	CCWGG	4 cut(s)	351, 645, 825, 854
MvnI	CGCG	1 cut(s)	753
MwoI	GCNNNNNNNGC	8 cut(s)	45, 346, 739, 1106, 1132, 1138, 1223, 1245
NciI	CCSGG	1 cut(s)	791
NdeI	CATATG	1 cut(s)	727
NdeII	GATC	2 cut(s)	309, 539
NheI	GCTAGC	1 cut(s)	161
NlaIII	CATG	8 cut(s)	107, 128, 225, 242, 252, 635, 781, 1010
NlaIV	GGNNCC	1 cut(s)	1028
NsiI	ATGCAT	2 cut(s)	771, 783
NspI	RCATGY	3 cut(s)	242, 252, 635
PciI	ACATGT	1 cut(s)	631
PctI	GAATGC	1 cut(s)	781
PdmI	GAANNNNTTC	1 cut(s)	633
PkrI	GCNGC	8 cut(s)	348, 548, 612, 704, 1087, 1102, 1134, 1247
PleI	GAGTC	3 cut(s)	360, 475, 854
PpsI	GAGTC	3 cut(s)	360, 475, 854
PscI	ACATGT	1 cut(s)	631
Psp124BI	GAGCTC	1 cut(s)	1143
Psp6I	CCWGG	4 cut(s)	349, 643, 823, 852
PspFI	CCCAGC	1 cut(s)	742
PspGI	CCWGG	4 cut(s)	349, 643, 823, 852
PspN4I	GGNNCC	1 cut(s)	1028
PspPI	GGNCC	1 cut(s)	755
PsrI	GAACNNNNNNTAC	2 cut(s)	481, 513
PsuI	RGATCY	1 cut(s)	309
RsaI	GTAC	4 cut(s)	499, 817, 919, 942
RsaNI	GTAC	4 cut(s)	498, 816, 918, 941
SacI	GAGCTC	1 cut(s)	1143
SaqAI	TTAA	7 cut(s)	113, 297, 384, 404, 566, 666, 1065
SatI	GCNGC	8 cut(s)	347, 547, 611, 703, 1086, 1101, 1133, 1246
Sau3AI	GATC	2 cut(s)	309, 539
Sau96I	GGNCC	1 cut(s)	755
SchI	GAGTC	3 cut(s)	360, 475, 854
ScrFI	CCNGG	5 cut(s)	351, 645, 791, 825, 854
SduI	GDGCHC	5 cut(s)	221, 749, 853, 1111, 1143
SfaNI	GCATC	4 cut(s)	511, 756, 783, 796
SinI	GGWCC	1 cut(s)	755
SmlI	CTYRAG	1 cut(s)	1240
SmoI	CTYRAG	1 cut(s)	1240
Sse9I	AATT	8 cut(s)	400, 458, 590, 667, 908, 933, 1013, 1259
SsiI	CCGC	5 cut(s)	171, 315, 649, 753, 1133
SspMI	CTAG	5 cut(s)	54, 162, 443, 537, 759
SstI	GAGCTC	1 cut(s)	1143
StyD4I	CCNGG	5 cut(s)	349, 643, 789, 823, 852
StyI	CCWWGG	1 cut(s)	975
TaaI	ACNGT	4 cut(s)	152, 820, 1020, 1054
TaqI	TCGA	4 cut(s)	232, 484, 1143, 1251
TasI	AATT	8 cut(s)	400, 458, 590, 667, 908, 933, 1013, 1259
TatI	WGTACW	2 cut(s)	497, 917
TauI	GCSGC	1 cut(s)	1135
Tru1I	TTAA	7 cut(s)	113, 297, 384, 404, 566, 666, 1065
Tru9I	TTAA	7 cut(s)	113, 297, 384, 404, 566, 666, 1065
TscAI	CASTG	1 cut(s)	1025
TseI	GCWGC	7 cut(s)	346, 546, 610, 702, 1085, 1100, 1245
TspDTI	ATGAA	3 cut(s)	370, 583, 1023
TspRI	CASTG	1 cut(s)	1025
VneI	GTGCAC	1 cut(s)	1107
VpaK11BI	GGWCC	1 cut(s)	755
XapI	RAATTY	3 cut(s)	458, 590, 908
XbaI	TCTAGA	2 cut(s)	53, 536
XceI	RCATGY	3 cut(s)	242, 252, 635
XmiI	GTMKAC	1 cut(s)	600
XmnI	GAANNNNTTC	1 cut(s)	633
XspI	CTAG	5 cut(s)	54, 162, 443, 537, 759
Zsp2I	ATGCAT	2 cut(s)	771, 783

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.