pycom08g19550

Tubby-like F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0007029
Physical Location & Seq
Forward (+)
19596257 .. 19596766
510 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g19550.1

Sequence Viewer

Length: 510 bp
ATGCATTGCATCATGCATTCTATCCCGGTCTCATCTCTTGATGCAGGAGGTACTGTCCCTGGCCAACCAGAACTCCTTACCCGAGCCCTGGAAGACTCAGTCCGAAGTATCTCTTTCTCAAAGTCTCTTGACCACTCAGTAGAATTCAGCAGTACACGGTTTTCTGAAATTGATGTACCCCGAGATGATGAGGATGGCAAGACAAGACCCTTGGTTTTGAAAAACAAACCCCCACGATGGCATGAACAATTACAGTGTTGGTGCCTGAACTTCCGAGGCAGGGTAACTGTTGCTTCTGTTAAGAACTTTCAGTTGATTGCTGCTACTCAGCCTGCTGCTGGTGCACCTACTCCATCACAGCCAGCGCCGCCAGAGCACGACAAGATACTCTTGCAGTTTGGCAAGGTTGGTAAAGATATGTTCACGATGGATTATCGCTATCCTTTATCTGCATTTCAGGCTTTCGCTATCTGCTTGAGCAGCTTTGACACGAAATTGGCTTGTGAATAG

Protein Analysis

170

Amino Acids

18.75

Weight (kDa)

6.51

Isoelectric Point (pI)

54.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tub PF01167 1 - 164 9.2e-54 Tub family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 261
AciI CCGC 1 cut(s) 368
AcoI YGGCCR 1 cut(s) 61
AcsI RAATTY 1 cut(s) 143
AfaI GTAC 3 cut(s) 52, 154, 177
AfiI CCNNNNNNNGG 4 cut(s) 88, 237, 280, 338
AgsI TTSAA 1 cut(s) 220
AjnI CCWGG 2 cut(s) 58, 87
AluBI AGCT 1 cut(s) 483
AluI AGCT 1 cut(s) 483
Alw21I GWGCWC 2 cut(s) 346, 378
Alw26I GTCTC 2 cut(s) 34, 129
Alw44I GTGCAC 1 cut(s) 342
Ama87I CYCGRG 2 cut(s) 81, 180
AoxI GGCC 1 cut(s) 61
ApaLI GTGCAC 1 cut(s) 342
ApeKI GCWGC 3 cut(s) 320, 335, 480
ApoI RAATTY 1 cut(s) 143
AspLEI GCGC 1 cut(s) 367
AsuC2I CCSGG 1 cut(s) 26
AvaI CYCGRG 2 cut(s) 81, 180
BaeGI GKGCMC 1 cut(s) 346
BalI TGGCCA 1 cut(s) 63
BanI GGYRCC 1 cut(s) 261
BanII GRGCYC 1 cut(s) 88
BbsI GAAGAC 1 cut(s) 99
Bbv12I GWGCWC 2 cut(s) 346, 378
BbvI GCAGC 3 cut(s) 307, 322, 492
BccI CCATC 4 cut(s) 188, 231, 361, 421
BciT130I CCWGG 2 cut(s) 60, 89
BcnI CCSGG 1 cut(s) 26
BcoDI GTCTC 2 cut(s) 34, 129
BfoI RGCGCY 1 cut(s) 368
BisI GCNGC 4 cut(s) 321, 336, 368, 481
BlsI GCNGC 4 cut(s) 322, 337, 369, 482
Bme1390I CCNGG 3 cut(s) 26, 60, 89
BmeT110I CYCGRG 2 cut(s) 81, 180
BmiI GGNNCC 1 cut(s) 263
BmrFI CCNGG 3 cut(s) 26, 60, 89
BmsI GCATC 2 cut(s) 18, 31
BpiI GAAGAC 1 cut(s) 99
BpuEI CTTGAG 1 cut(s) 496
BpuMI CCSGG 1 cut(s) 26
BsaI GGTCTC 1 cut(s) 34
BsaJI CCNNGG 4 cut(s) 58, 87, 210, 274
BsaXI ACNNNNNCTCC 4 cut(s) 39, 57, 69, 87
Bsc4I CCNNNNNNNGG 4 cut(s) 88, 237, 280, 338
Bse3DI GCAATG 1 cut(s) 4
BseBI CCWGG 2 cut(s) 60, 89
BseDI CCNNGG 4 cut(s) 58, 87, 210, 274
BseGI GGATG 1 cut(s) 199
BseLI CCNNNNNNNGG 4 cut(s) 88, 237, 280, 338
BseMI GCAATG 1 cut(s) 4
BseMII CTCAG 3 cut(s) 111, 150, 341
BseSI GKGCMC 1 cut(s) 346
BseXI GCAGC 3 cut(s) 307, 322, 492
BshFI GGCC 1 cut(s) 63
BshNI GGYRCC 1 cut(s) 261
BsiHKAI GWGCWC 2 cut(s) 346, 378
BsiHKCI CYCGRG 2 cut(s) 81, 180
BsiSI CCGG 1 cut(s) 26
BslFI GGGAC 1 cut(s) 41
BslI CCNNNNNNNGG 4 cut(s) 88, 237, 280, 338
BsmAI GTCTC 2 cut(s) 34, 129
BsmFI GGGAC 1 cut(s) 41
BsmI GAATGC 1 cut(s) 16
BsnI GGCC 1 cut(s) 63
Bso31I GGTCTC 1 cut(s) 34
BsoBI CYCGRG 2 cut(s) 81, 180
Bsp1286I GDGCHC 3 cut(s) 88, 346, 378
BspACI CCGC 1 cut(s) 368
BspANI GGCC 1 cut(s) 63
BspCNI CTCAG 3 cut(s) 110, 149, 340
BspLI GGNNCC 1 cut(s) 263
BspT107I GGYRCC 1 cut(s) 261
BspTNI GGTCTC 1 cut(s) 34
BsrDI GCAATG 1 cut(s) 4
BssECI CCNNGG 4 cut(s) 58, 87, 210, 274
BssT1I CCWWGG 1 cut(s) 210
Bst2UI CCWGG 2 cut(s) 60, 89
Bst4CI ACNGT 4 cut(s) 55, 159, 255, 289
BstC8I GCNNGC 2 cut(s) 333, 363
BstDEI CTNAG 3 cut(s) 97, 136, 327
BstF5I GGATG 1 cut(s) 199
BstH2I RGCGCY 1 cut(s) 368
BstHHI GCGC 1 cut(s) 367
BstMAI GTCTC 2 cut(s) 34, 129
BstMWI GCNNNNNNNGC 5 cut(s) 341, 367, 373, 458, 480
BstNI CCWGG 2 cut(s) 60, 89
BstSCI CCNGG 3 cut(s) 24, 58, 87
BstSLI GKGCMC 1 cut(s) 346
BstV1I GCAGC 3 cut(s) 307, 322, 492
BstV2I GAAGAC 1 cut(s) 99
BsuRI GGCC 1 cut(s) 63
BtsCI GGATG 1 cut(s) 199
BtsIMutI CAGTG 1 cut(s) 260
Cac8I GCNNGC 2 cut(s) 333, 363
CfoI GCGC 1 cut(s) 367
Csp6I GTAC 3 cut(s) 51, 153, 176
CviAII CATG 2 cut(s) 13, 242
CviJI RGCY 7 cut(s) 63, 86, 331, 361, 461, 483, 500
CviKI_1 RGCY 7 cut(s) 63, 86, 331, 361, 461, 483, 500
CviQI GTAC 3 cut(s) 51, 153, 176
DdeI CTNAG 3 cut(s) 97, 136, 327
EaeI YGGCCR 1 cut(s) 61
Eco130I CCWWGG 1 cut(s) 210
Eco24I GRGCYC 1 cut(s) 88
Eco31I GGTCTC 1 cut(s) 34
Eco88I CYCGRG 2 cut(s) 81, 180
EcoRI GAATTC 1 cut(s) 143
EcoRII CCWGG 2 cut(s) 58, 87
EcoT14I CCWWGG 1 cut(s) 210
EcoT22I ATGCAT 2 cut(s) 6, 18
EcoT38I GRGCYC 1 cut(s) 88
ErhI CCWWGG 1 cut(s) 210
FaeI CATG 2 cut(s) 16, 245
FaiI YATR 3 cut(s) 14, 243, 419
FalI AAGNNNNNCTT 4 cut(s) 97, 129, 374, 406
FaqI GGGAC 1 cut(s) 41
FatI CATG 2 cut(s) 12, 241
Fnu4HI GCNGC 4 cut(s) 321, 336, 368, 481
FokI GGATG 1 cut(s) 206
FriOI GRGCYC 1 cut(s) 88
Fsp4HI GCNGC 4 cut(s) 321, 336, 368, 481
GlaI GCGC 1 cut(s) 366
GluI GCNGC 4 cut(s) 321, 336, 368, 481
HaeII RGCGCY 1 cut(s) 368
HaeIII GGCC 1 cut(s) 63
HapII CCGG 1 cut(s) 26
HhaI GCGC 1 cut(s) 367
Hin1II CATG 2 cut(s) 16, 245
Hin6I GCGC 1 cut(s) 365
HinP1I GCGC 1 cut(s) 365
HinfI GANTC 1 cut(s) 95
HpaII CCGG 1 cut(s) 26
Hpy166II GTNNAC 3 cut(s) 155, 344, 423
Hpy188I TCNGA 3 cut(s) 104, 166, 275
Hpy188III TCNNGA 3 cut(s) 38, 128, 424
Hpy8I GTNNAC 3 cut(s) 155, 344, 423
HpyCH4III ACNGT 4 cut(s) 55, 159, 255, 289
HpyCH4V TGCA 7 cut(s) 4, 9, 16, 44, 344, 394, 452
HpyF10VI GCNNNNNNNGC 5 cut(s) 341, 367, 373, 458, 480
HpyF3I CTNAG 3 cut(s) 97, 136, 327
Hsp92II CATG 2 cut(s) 16, 245
HspAI GCGC 1 cut(s) 365
Lsp1109I GCAGC 3 cut(s) 307, 322, 492
LweI GCATC 2 cut(s) 18, 31
MaeIII GTNAC 1 cut(s) 283
MboII GAAGA 1 cut(s) 104
MhlI GDGCHC 3 cut(s) 88, 346, 378
MlsI TGGCCA 1 cut(s) 63
MluCI AATT 4 cut(s) 143, 168, 248, 494
MluNI TGGCCA 1 cut(s) 63
MlyI GAGTC 1 cut(s) 89
MnlI CCTC 3 cut(s) 41, 184, 269
Mox20I TGGCCA 1 cut(s) 63
Mph1103I ATGCAT 2 cut(s) 6, 18
MscI TGGCCA 1 cut(s) 63
MseI TTAA 1 cut(s) 300
Msp20I TGGCCA 1 cut(s) 63
MspI CCGG 1 cut(s) 26
MspR9I CCNGG 3 cut(s) 26, 60, 89
Mva1269I GAATGC 1 cut(s) 16
MvaI CCWGG 2 cut(s) 60, 89
MwoI GCNNNNNNNGC 5 cut(s) 341, 367, 373, 458, 480
NciI CCSGG 1 cut(s) 26
NlaIII CATG 2 cut(s) 16, 245
NlaIV GGNNCC 1 cut(s) 263
NsiI ATGCAT 2 cut(s) 6, 18
PctI GAATGC 1 cut(s) 16
PflFI GACNNNGTC 1 cut(s) 98
PkrI GCNGC 4 cut(s) 322, 337, 369, 482
PleI GAGTC 1 cut(s) 89
PpsI GAGTC 1 cut(s) 89
Psp6I CCWGG 2 cut(s) 58, 87
PspGI CCWGG 2 cut(s) 58, 87
PspN4I GGNNCC 1 cut(s) 263
PsyI GACNNNGTC 1 cut(s) 98
RsaI GTAC 3 cut(s) 52, 154, 177
RsaNI GTAC 3 cut(s) 51, 153, 176
SaqAI TTAA 1 cut(s) 300
SatI GCNGC 4 cut(s) 321, 336, 368, 481
SchI GAGTC 1 cut(s) 89
ScrFI CCNGG 3 cut(s) 26, 60, 89
SduI GDGCHC 3 cut(s) 88, 346, 378
SetI ASST 4 cut(s) 52, 349, 408, 485
SfaNI GCATC 2 cut(s) 18, 31
SmlI CTYRAG 1 cut(s) 475
SmoI CTYRAG 1 cut(s) 475
Sse9I AATT 4 cut(s) 143, 168, 248, 494
SsiI CCGC 1 cut(s) 368
StyD4I CCNGG 3 cut(s) 24, 58, 87
StyI CCWWGG 1 cut(s) 210
TaaI ACNGT 4 cut(s) 55, 159, 255, 289
TasI AATT 4 cut(s) 143, 168, 248, 494
TatI WGTACW 1 cut(s) 152
TauI GCSGC 1 cut(s) 370
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TscAI CASTG 1 cut(s) 260
TseI GCWGC 3 cut(s) 320, 335, 480
TspDTI ATGAA 1 cut(s) 258
TspRI CASTG 1 cut(s) 260
Tth111I GACNNNGTC 1 cut(s) 98
VneI GTGCAC 1 cut(s) 342
XapI RAATTY 1 cut(s) 143
Zsp2I ATGCAT 2 cut(s) 6, 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.