AT1G25540

Mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005569
Physical Location & Seq
Reverse (-)
8969065 .. 8974815
5751 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G25540.2

Sequence Viewer

Length: 2511 bp
ATGTCGTCGGAGGTGAAACAGCTGATCGTCGTTGCTGAAGGCACCGCCGCTTTGGGTCCTTATTGGCAAACCATCGTCTCCGACTATCTCGAGAAAATCATCAGGTCTTTCTGTGGCAGTGAGTTAAATGGAGAGAGGAACCCTGTTTCTACTGTTGAGCTATCACTGGTGATCTTCAATTCTCATGGTTCATATTGTGCTTGCTTGGTACAACGGAGTGGCTGGACAAGGGATGTTGATATTTTCTTGCATTGGCTTTCTTCCATACAATTTGGTGGTGGTGGTTTCAATGAGGTTGCCACAGCTGAAGGGCTTGCCGAAGCATTGATGATGTTTTCTCCTCCTTCAGGCCAAGCTCAACCAAGTAACGATCTGAAAAGACACTGTATCCTAATCACAGCCAGCAATCCTCACATATTGCCAACACCTGTATATCGTCCACGATTGCAAAATGTGGAACGGAATGAGAATGGTGATGCGCAAGCTGAGAGTCGATTATCAGATGCTGAGACAGTGGCTTCATATTTTGCTAAGTGTTCTGTTTCTTTGTCTGTTGTATGTCCAAAGCAGCTTCCAACAATTAGAGCACTATACAATGCGGGAAAGCCCAATCAACAAAGTGCGGACTTGTCAATTGACACGGCTAAGAACACATTCTATCTTGTCCTGATCTCGGAGAATTTTGTGGAGGCATGTGCTGCCTTAAGTCATTCTGCTACAAATTTGCCACAGACTCAGAGCCCTGTGAAAGTGGACAGGGCCACTGTTGCTCCATCTATTCCAGTCACTGGGCAACCTCCAGCTCCTGTGTCATCAGCCAATGGACCTATTCAGAATCGGCAACCAGTTTCTGTTGGACCAGTTCCAACTGCTACTGTGAAAGTTGAGCCTAGCACCGTAACTTCTATGGCACCAGTTCCTAGTTTTCCCCATATCCCGGCTGTAGCTCGGCCTGCTACACAAGCAATTCCTTCGATTCAAACATCTTCAGCATCACCAGTTTCTCAGGATATGGTCAGCAACGCCGAGAATGCACCAGATATTAAGCCTGTGGTGGTCAGTGGAATGACGCCACCATTGCGTACTGGTCCTCCTGGTGGAGCCAATGTAAATCTGCTTAATAATCTTTCTCAAGTCCGACAAGTCATGAGCTCTGCAGCTCTGGCAGGTGCAGCCTCATCGGTTGGGCAAAGTGCGGTTGCAATGCATATGTCAAATATGATATCAACAGGAATGGCTACATCTTTGCCTCCTTCACAAACTGTGTTTTCAACTGGACAGCAGGGAATTACTTCAATGGCTGGTTCGGGTGCACTAATGGGATCTGCACAAACGGGACAAAGCCCGGGTCCTAATAATGCCTTTAGTCCTCAAACAACGTCAAATGTCGCTTCAAACCTTGGTGTTTCACAACCAATGCAAGGGATGAACCAAGGAAGTCATTCTGGAGCAATGATGCAAGGTGGAATTTCCATGAACCAAAACATGATGAGTGGTCTTGGTCAAGGAAATGTCTCCTCTGGAACAGGTGGAATGATGCCTACTCCAGGAGTTGGCCAACAAGCGCAATCAGGAATACAACAACTTGGTGGCAGTAACAGCTCAGCTCCTAATATGCAGCTATCACAGCCATCATCGGGGGCTATGCAGACTTCACAATCCAAATATGTGAAAGTCTGGGAGGGAAATTTATCTGGGCAAAGGCAAGGGCAGCCTGTTCTTATCACCAGACTTGAGGGTTACCGAAGTGCTTCTGCCTCTGATTCGTTGGCAGCAAACTGGCCACCAACTATGCAGATTGTTCGTCTCATATCCCAGGACCATATGAATAACAAGCAATATGTTGGCAAAGCTGACTTCCTTGTGTTTCGGGCCATGAGTCAACATGGGTTCTTAGGACAACTTCAGGATAAAAAGCTTTGTGCAGTCATCCAGTTGCCATCACAGACGCTTCTTCTCTCTGTCTCTGACAAGGCTTGCCGCTTGATTGGAATGCTTTTCCCAGGGGATATGGTTGTGTTTAAACCACAAATTCCAAATCAGCAACAGCAGCAGCAACAACAACTCCACCAGCAACAACAACAACAACAGCAGATCCAGCAGCAGCAGCAACAACAACAACACCTCCAACAGCAACAGATGCCACAACTCCAGCAACAACAACAACAACACCAGCAGCAACAGCAACAGCAGCATCAATTGTCACAGCTCCAACATCATCAGCAGCAACAACAACAACAGCAGCAACAACAGCAGCAGCATCAATTGACACAGCTTCAACACCATCATCAGCAGCAGCAGCAGGCGTCGCCGCTGAATCAGATGCAGCAGCAGACTTCGCCGCTCAATCAGATGCAGCAACAGACTTCGCCTCTGAATCAGATGCAGCAGCAACAGCAGCCTCAACAGATGGTAATGGGTGGTCAAGCTTTTGCACAAGCCCCTGGAAGATCACAACAAGGTGGTGGTGGAGGGCAGCCTAACATGCCTGGAGCTGGCTTCATGGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000902 GO:0000904 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003712 GO:0003713 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006355 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007602 GO:0008150 GO:0009314 GO:0009416 GO:0009581 GO:0009582 GO:0009583 GO:0009585 GO:0009605 GO:0009607 GO:0009620 GO:0009628 GO:0009639 GO:0009653 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009867 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009909 GO:0009911 GO:0009987 GO:0010017 GO:0010026 GO:0010033 GO:0010090 GO:0010091 GO:0010114 GO:0010218 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010927 GO:0016043 GO:0016592 GO:0019219 GO:0019222 GO:0022607 GO:0023052 GO:0030030 GO:0030031 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031349 GO:0031974 GO:0031981 GO:0032502 GO:0032870 GO:0032989 GO:0032991 GO:0042221 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045893 GO:0045935 GO:0048468 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048583 GO:0048584 GO:0048646 GO:0048831 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051094 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051606 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0071214 GO:0071229 GO:0071310 GO:0071395 GO:0071478 GO:0071482 GO:0071489 GO:0071495 GO:0071840 GO:0080090 GO:0080134 GO:0090558 GO:0090626 GO:0097159 GO:0098542 GO:0104004 GO:0140110 GO:1901363 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:1905499 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med25_VWA

Protein Analysis

836

Amino Acids

89.56

Weight (kDa)

8.51

Isoelectric Point (pI)

61.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med25_VWA PF11265 11 - 117 1.4e-14 Mediator complex subunit 25 von Willebrand factor type A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1157
Acc16I TGCGCA 1 cut(s) 480
Acc36I ACCTGC 1 cut(s) 1157
AccB1I GGYRCC 2 cut(s) 41, 910
AccB7I CCANNNNNTGG 2 cut(s) 788, 1553
AccBSI CCGCTC 1 cut(s) 2344
AciI CCGC 8 cut(s) 45, 48, 599, 623, 1196, 1981, 2312, 2342
AclWI GGATC 2 cut(s) 1330, 2089
AcoI YGGCCR 2 cut(s) 1555, 1781
AcsI RAATTY 5 cut(s) 679, 721, 1467, 1687, 2031
AcuI CTGAAG 5 cut(s) 57, 327, 330, 972, 1889
AcyI GRCGYC 2 cut(s) 1070, 2306
AfaI GTAC 2 cut(s) 210, 1084
AflII CTTAAG 1 cut(s) 703
AgsI TTSAA 7 cut(s) 178, 289, 980, 1272, 1296, 1395, 2279
AjnI CCWGG 6 cut(s) 1093, 1546, 1815, 2002, 2443, 2488
Alw21I GWGCWC 3 cut(s) 589, 1154, 1315
Alw26I GTCTC 5 cut(s) 82, 503, 1519, 1811, 1969
Alw44I GTGCAC 1 cut(s) 1311
AlwI GGATC 2 cut(s) 1330, 2089
AlwNI CAGNNNCTG 4 cut(s) 506, 788, 806, 851
Ama87I CYCGRG 2 cut(s) 89, 1345
AoxI GGCC 6 cut(s) 349, 759, 950, 1555, 1781, 1872
ApaLI GTGCAC 1 cut(s) 1311
ApoI RAATTY 5 cut(s) 679, 721, 1467, 1687, 2031
ArsI GACNNNNNNTTYG 2 cut(s) 1333, 1365
Asp700I GAANNNNTTC 4 cut(s) 653, 1291, 1441, 1750
AspLEI GCGC 2 cut(s) 481, 1567
AspS9I GGNCC 8 cut(s) 56, 759, 824, 857, 1088, 1349, 1819, 1872
AsuC2I CCSGG 3 cut(s) 938, 1346, 1347
AsuHPI GGTGA 5 cut(s) 25, 181, 485, 987, 1717
AvaI CYCGRG 2 cut(s) 89, 1345
AvaII GGWCC 6 cut(s) 56, 824, 857, 1088, 1349, 1819
BaeGI GKGCMC 1 cut(s) 1315
BalI TGGCCA 2 cut(s) 1557, 1783
BanI GGYRCC 2 cut(s) 41, 910
BanII GRGCYC 2 cut(s) 743, 1154
Bbv12I GWGCWC 3 cut(s) 589, 1154, 1315
BccI CCATC 6 cut(s) 80, 781, 1639, 1948, 2292, 2404
BceAI ACGGC 1 cut(s) 657
BcgI CGANNNNNNTGC 6 cut(s) 954, 988, 1161, 1195, 1784, 1818
BciT130I CCWGG 6 cut(s) 1095, 1548, 1817, 2004, 2445, 2490
BciVI GTATCC 1 cut(s) 398
BcnI CCSGG 3 cut(s) 938, 1346, 1347
BcoDI GTCTC 5 cut(s) 82, 503, 1519, 1811, 1969
BfaI CTAG 2 cut(s) 891, 921
BfmI CTRYAG 2 cut(s) 942, 1155
BfrI CTTAAG 1 cut(s) 703
BfuAI ACCTGC 1 cut(s) 1157
BfuI GTATCC 1 cut(s) 398
BlpI GCTNAGC 1 cut(s) 1603
Bme1390I CCNGG 9 cut(s) 938, 1095, 1346, 1347, 1548, 1817, 2004, 2445, 2490
Bme18I GGWCC 6 cut(s) 56, 824, 857, 1088, 1349, 1819
BmeT110I CYCGRG 2 cut(s) 89, 1345
BmgT120I GGNCC 8 cut(s) 56, 759, 824, 857, 1088, 1349, 1819, 1872
BmiI GGNNCC 6 cut(s) 43, 57, 140, 912, 1102, 1350
BmrFI CCNGG 9 cut(s) 938, 1095, 1346, 1347, 1548, 1817, 2004, 2445, 2490
BmrI ACTGGG 1 cut(s) 798
BmuI ACTGGG 1 cut(s) 798
BpmI CTGGAG 5 cut(s) 783, 1467, 1530, 2135, 2511
Bpu1102I GCTNAGC 1 cut(s) 1603
BpuEI CTTGAG 2 cut(s) 1116, 1754
BpuMI CCSGG 3 cut(s) 938, 1346, 1347
BsaHI GRCGYC 2 cut(s) 1070, 2306
BsaJI CCNNGG 7 cut(s) 1345, 1399, 1432, 1815, 2002, 2003, 2443
Bse3DI GCAATG 3 cut(s) 1076, 1209, 1458
BseBI CCWGG 6 cut(s) 1095, 1548, 1817, 2004, 2445, 2490
BseDI CCNNGG 7 cut(s) 1345, 1399, 1432, 1815, 2002, 2003, 2443
BseGI GGATG 3 cut(s) 238, 1431, 1929
BseMI GCAATG 3 cut(s) 1076, 1209, 1458
BseMII CTCAG 5 cut(s) 477, 498, 749, 1019, 1617
BseRI GAGGAG 2 cut(s) 330, 1507
BseSI GKGCMC 1 cut(s) 1315
BsgI GTGCAG 3 cut(s) 1191, 1311, 1944
BshFI GGCC 6 cut(s) 351, 761, 952, 1557, 1783, 1874
BshNI GGYRCC 2 cut(s) 41, 910
BsiHKAI GWGCWC 3 cut(s) 589, 1154, 1315
BsiHKCI CYCGRG 2 cut(s) 89, 1345
BsiSI CCGG 2 cut(s) 938, 1346
BslFI GGGAC 1 cut(s) 1350
BsmAI GTCTC 5 cut(s) 82, 503, 1519, 1811, 1969
BsmBI CGTCTC 2 cut(s) 82, 1811
BsmFI GGGAC 1 cut(s) 1350
BsmI GAATGC 2 cut(s) 1036, 1998
BsnI GGCC 6 cut(s) 351, 761, 952, 1557, 1783, 1874
BsoBI CYCGRG 2 cut(s) 89, 1345
Bsp1286I GDGCHC 4 cut(s) 589, 743, 1154, 1315
Bsp143I GATC 7 cut(s) 24, 171, 370, 669, 1322, 2094, 2450
Bsp1720I GCTNAGC 1 cut(s) 1603
BspACI CCGC 8 cut(s) 45, 48, 599, 623, 1196, 1981, 2312, 2342
BspANI GGCC 6 cut(s) 351, 761, 952, 1557, 1783, 1874
BspCNI CTCAG 5 cut(s) 478, 499, 748, 1018, 1616
BspHI TCATGA 1 cut(s) 1146
BspLI GGNNCC 6 cut(s) 43, 57, 140, 912, 1102, 1350
BspMAI CTGCAG 1 cut(s) 1159
BspMI ACCTGC 1 cut(s) 1157
BspPI GGATC 2 cut(s) 1330, 2089
BspT107I GGYRCC 2 cut(s) 41, 910
BspTI CTTAAG 1 cut(s) 703
BsrBI CCGCTC 1 cut(s) 2344
BsrDI GCAATG 3 cut(s) 1076, 1209, 1458
BssECI CCNNGG 7 cut(s) 1345, 1399, 1432, 1815, 2002, 2003, 2443
BssMI GATC 7 cut(s) 24, 171, 370, 669, 1322, 2094, 2450
BssNI GRCGYC 2 cut(s) 1070, 2306
BssT1I CCWWGG 2 cut(s) 1399, 1432
Bst2UI CCWGG 6 cut(s) 1095, 1548, 1817, 2004, 2445, 2490
Bst4CI ACNGT 7 cut(s) 154, 386, 514, 766, 877, 898, 1264
BstACI GRCGYC 2 cut(s) 1070, 2306
BstAFI CTTAAG 1 cut(s) 703
BstAPI GCANNNNNTGC 2 cut(s) 698, 2140
BstC8I GCNNGC 8 cut(s) 202, 315, 403, 483, 954, 1978, 2304, 2497
BstDEI CTNAG 8 cut(s) 486, 507, 531, 645, 735, 1005, 1603, 1894
BstEII GGTNACC 1 cut(s) 1739
BstENI CCTNNNNNAGG 2 cut(s) 345, 1545
BstF5I GGATG 3 cut(s) 238, 1431, 1929
BstHHI GCGC 2 cut(s) 481, 1567
BstKTI GATC 7 cut(s) 27, 174, 373, 672, 1325, 2097, 2453
BstMAI GTCTC 5 cut(s) 82, 503, 1519, 1811, 1969
BstMBI GATC 7 cut(s) 24, 171, 370, 669, 1322, 2094, 2450
BstNI CCWGG 6 cut(s) 1095, 1548, 1817, 2004, 2445, 2490
BstNSI RCATGY 2 cut(s) 696, 2488
BstPI GGTNACC 1 cut(s) 1739
BstSCI CCNGG 9 cut(s) 936, 1093, 1344, 1345, 1546, 1815, 2002, 2443, 2488
BstSFI CTRYAG 2 cut(s) 942, 1155
BstSLI GKGCMC 1 cut(s) 1315
BstX2I RGATCY 2 cut(s) 1322, 2094
BstYI RGATCY 2 cut(s) 1322, 2094
BsuI GTATCC 1 cut(s) 398
BsuRI GGCC 6 cut(s) 351, 761, 952, 1557, 1783, 1874
BtsCI GGATG 3 cut(s) 238, 1431, 1929
BtsI GCAGTG 1 cut(s) 124
BtsIMutI CAGTG 7 cut(s) 124, 164, 382, 519, 762, 786, 1066
BveI ACCTGC 1 cut(s) 1157
Cac8I GCNNGC 8 cut(s) 202, 315, 403, 483, 954, 1978, 2304, 2497
CaiI CAGNNNCTG 4 cut(s) 506, 788, 806, 851
CciI TCATGA 1 cut(s) 1146
CfoI GCGC 2 cut(s) 481, 1567
Cfr13I GGNCC 8 cut(s) 56, 759, 824, 857, 1088, 1349, 1819, 1872
Cfr9I CCCGGG 1 cut(s) 1345
CseI GACGC 3 cut(s) 1078, 1957, 2295
Csp6I GTAC 2 cut(s) 209, 1083
CviAII CATG 9 cut(s) 185, 693, 1147, 1474, 1486, 1876, 1886, 2485, 2503
CviQI GTAC 2 cut(s) 209, 1083
DdeI CTNAG 8 cut(s) 486, 507, 531, 645, 735, 1005, 1603, 1894
DpnI GATC 7 cut(s) 26, 173, 372, 671, 1324, 2096, 2452
DpnII GATC 7 cut(s) 24, 171, 370, 669, 1322, 2094, 2450
DraI TTTAAA 1 cut(s) 2023
EaeI YGGCCR 2 cut(s) 1555, 1781
Ecl136II GAGCTC 1 cut(s) 1152
Eco130I CCWWGG 2 cut(s) 1399, 1432
Eco24I GRGCYC 2 cut(s) 743, 1154
Eco32I GATATC 1 cut(s) 1224
Eco47I GGWCC 6 cut(s) 56, 824, 857, 1088, 1349, 1819
Eco53kI GAGCTC 1 cut(s) 1152
Eco57I CTGAAG 5 cut(s) 57, 327, 330, 972, 1889
Eco88I CYCGRG 2 cut(s) 89, 1345
Eco91I GGTNACC 1 cut(s) 1739
EcoICRI GAGCTC 1 cut(s) 1152
EcoNI CCTNNNNNAGG 2 cut(s) 345, 1545
EcoO109I RGGNCCY 2 cut(s) 56, 1349
EcoO65I GGTNACC 1 cut(s) 1739
EcoRII CCWGG 6 cut(s) 1093, 1546, 1815, 2002, 2443, 2488
EcoRV GATATC 1 cut(s) 1224
EcoT14I CCWWGG 2 cut(s) 1399, 1432
EcoT22I ATGCAT 1 cut(s) 1209
EcoT38I GRGCYC 2 cut(s) 743, 1154
ErhI CCWWGG 2 cut(s) 1399, 1432
Esp3I CGTCTC 2 cut(s) 82, 1811
FaeI CATG 9 cut(s) 188, 696, 1150, 1477, 1489, 1879, 1889, 2488, 2506
FaqI GGGAC 1 cut(s) 1350
FatI CATG 9 cut(s) 184, 692, 1146, 1473, 1485, 1875, 1885, 2484, 2502
FauI CCCGC 1 cut(s) 592
FauNDI CATATG 2 cut(s) 1209, 1824
FokI GGATG 3 cut(s) 245, 1438, 1916
FriOI GRGCYC 2 cut(s) 743, 1154
FspBI CTAG 2 cut(s) 891, 921
FspI TGCGCA 1 cut(s) 480
GlaI GCGC 2 cut(s) 480, 1566
GsuI CTGGAG 5 cut(s) 783, 1467, 1530, 2135, 2511
HaeIII GGCC 6 cut(s) 351, 761, 952, 1557, 1783, 1874
HapII CCGG 2 cut(s) 938, 1346
HgaI GACGC 3 cut(s) 1078, 1957, 2295
HhaI GCGC 2 cut(s) 481, 1567
Hin1I GRCGYC 2 cut(s) 1070, 2306
Hin1II CATG 9 cut(s) 188, 696, 1150, 1477, 1489, 1879, 1889, 2488, 2506
Hin6I GCGC 2 cut(s) 479, 1565
HinP1I GCGC 2 cut(s) 479, 1565
HincII GTYRAC 1 cut(s) 1883
HindII GTYRAC 1 cut(s) 1883
HindIII AAGCTT 2 cut(s) 1916, 2427
HinfI GANTC 8 cut(s) 490, 733, 835, 976, 1763, 1879, 2317, 2377
HpaII CCGG 2 cut(s) 938, 1346
HphI GGTGA 5 cut(s) 25, 181, 485, 987, 1717
Hpy166II GTNNAC 4 cut(s) 440, 754, 1313, 1883
Hpy188III TCNNGA 9 cut(s) 89, 91, 667, 1007, 1147, 1446, 1521, 1572, 1907
Hpy8I GTNNAC 4 cut(s) 440, 754, 1313, 1883
Hpy99I CGWCG 3 cut(s) 10, 32, 2311
HpyAV CCTTC 5 cut(s) 32, 302, 354, 981, 1263
HpyCH4III ACNGT 7 cut(s) 154, 386, 514, 766, 877, 898, 1264
HpyCH4IV ACGT 1 cut(s) 1379
HpyF3I CTNAG 8 cut(s) 486, 507, 531, 645, 735, 1005, 1603, 1894
HpySE526I ACGT 1 cut(s) 1379
Hsp92I GRCGYC 2 cut(s) 1070, 2306
Hsp92II CATG 9 cut(s) 188, 696, 1150, 1477, 1489, 1879, 1889, 2488, 2506
HspAI GCGC 2 cut(s) 479, 1565
Kzo9I GATC 7 cut(s) 24, 171, 370, 669, 1322, 2094, 2450
LmnI GCTCC 7 cut(s) 775, 808, 1100, 1448, 1612, 2214, 2492
MaeI CTAG 2 cut(s) 891, 921
MaeII ACGT 1 cut(s) 1379
MaeIII GTNAC 6 cut(s) 365, 784, 898, 1595, 1739, 2202
MalI GATC 7 cut(s) 26, 173, 372, 671, 1324, 2096, 2452
MbiI CCGCTC 1 cut(s) 2344
MboI GATC 7 cut(s) 24, 171, 370, 669, 1322, 2094, 2450
MboII GAAGA 5 cut(s) 166, 252, 978, 1946, 2460
MfeI CAATTG 3 cut(s) 633, 2198, 2264
MflI RGATCY 2 cut(s) 1322, 2094
MhlI GDGCHC 4 cut(s) 589, 743, 1154, 1315
MlsI TGGCCA 2 cut(s) 1557, 1783
MluNI TGGCCA 2 cut(s) 1557, 1783
MlyI GAGTC 3 cut(s) 499, 727, 1888
MmeI TCCRAC 7 cut(s) 105, 599, 835, 890, 1162, 2152, 2236
Mox20I TGGCCA 2 cut(s) 1557, 1783
Mph1103I ATGCAT 1 cut(s) 1209
MroXI GAANNNNTTC 4 cut(s) 653, 1291, 1441, 1750
MscI TGGCCA 2 cut(s) 1557, 1783
MseI TTAA 5 cut(s) 125, 704, 1044, 1119, 2022
Msp20I TGGCCA 2 cut(s) 1557, 1783
MspA1I CMGCKG 3 cut(s) 22, 305, 2314
MspCI CTTAAG 1 cut(s) 703
MspI CCGG 2 cut(s) 938, 1346
MspR9I CCNGG 9 cut(s) 938, 1095, 1346, 1347, 1548, 1817, 2004, 2445, 2490
MssI GTTTAAAC 1 cut(s) 2023
MunI CAATTG 3 cut(s) 633, 2198, 2264
Mva1269I GAATGC 2 cut(s) 1036, 1998
MvaI CCWGG 6 cut(s) 1095, 1548, 1817, 2004, 2445, 2490
NciI CCSGG 3 cut(s) 938, 1346, 1347
NdeI CATATG 2 cut(s) 1209, 1824
NdeII GATC 7 cut(s) 24, 171, 370, 669, 1322, 2094, 2450
NlaIII CATG 9 cut(s) 188, 696, 1150, 1477, 1489, 1879, 1889, 2488, 2506
NlaIV GGNNCC 6 cut(s) 43, 57, 140, 912, 1102, 1350
NmeAIII GCCGAG 2 cut(s) 928, 1051
NmuCI GTSAC 2 cut(s) 784, 2202
NsbI TGCGCA 1 cut(s) 480
NsiI ATGCAT 1 cut(s) 1209
NspI RCATGY 2 cut(s) 696, 2488
PaeR7I CTCGAG 1 cut(s) 89
PagI TCATGA 1 cut(s) 1146
PaqCI CACCTGC 1 cut(s) 1157
PasI CCCWGGG 1 cut(s) 2003
PctI GAATGC 2 cut(s) 1036, 1998
PdmI GAANNNNTTC 4 cut(s) 653, 1291, 1441, 1750
PfeI GAWTC 5 cut(s) 835, 976, 1763, 2317, 2377
PflMI CCANNNNNTGG 2 cut(s) 788, 1553
PfoI TCCNGGA 1 cut(s) 1546
PleI GAGTC 3 cut(s) 498, 727, 1887
PmeI GTTTAAAC 1 cut(s) 2023
PpsI GAGTC 3 cut(s) 498, 727, 1887
PpuMI RGGWCCY 2 cut(s) 56, 1349
Psp124BI GAGCTC 1 cut(s) 1154
Psp5II RGGWCCY 2 cut(s) 56, 1349
Psp6I CCWGG 6 cut(s) 1093, 1546, 1815, 2002, 2443, 2488
PspEI GGTNACC 1 cut(s) 1739
PspGI CCWGG 6 cut(s) 1093, 1546, 1815, 2002, 2443, 2488
PspN4I GGNNCC 6 cut(s) 43, 57, 140, 912, 1102, 1350
PspPI GGNCC 8 cut(s) 56, 759, 824, 857, 1088, 1349, 1819, 1872
PspPPI RGGWCCY 2 cut(s) 56, 1349
PstI CTGCAG 1 cut(s) 1159
PstNI CAGNNNCTG 4 cut(s) 506, 788, 806, 851
PsuI RGATCY 2 cut(s) 1322, 2094
PvuII CAGCTG 2 cut(s) 22, 305
RsaI GTAC 2 cut(s) 210, 1084
RsaNI GTAC 2 cut(s) 209, 1083
SacI GAGCTC 1 cut(s) 1154
SaqAI TTAA 5 cut(s) 125, 704, 1044, 1119, 2022
Sau3AI GATC 7 cut(s) 24, 171, 370, 669, 1322, 2094, 2450
Sau96I GGNCC 8 cut(s) 56, 759, 824, 857, 1088, 1349, 1819, 1872
SchI GAGTC 3 cut(s) 499, 727, 1888
ScrFI CCNGG 9 cut(s) 938, 1095, 1346, 1347, 1548, 1817, 2004, 2445, 2490
SduI GDGCHC 4 cut(s) 589, 743, 1154, 1315
SfcI CTRYAG 2 cut(s) 942, 1155
Sfr274I CTCGAG 1 cut(s) 89
SinI GGWCC 6 cut(s) 56, 824, 857, 1088, 1349, 1819
SlaI CTCGAG 1 cut(s) 89
SmaI CCCGGG 1 cut(s) 1347
SmlI CTYRAG 4 cut(s) 89, 703, 1131, 1733
SmoI CTYRAG 4 cut(s) 89, 703, 1131, 1733
SsiI CCGC 8 cut(s) 45, 48, 599, 623, 1196, 1981, 2312, 2342
SspMI CTAG 2 cut(s) 891, 921
SstI GAGCTC 1 cut(s) 1154
StyD4I CCNGG 9 cut(s) 936, 1093, 1344, 1345, 1546, 1815, 2002, 2443, 2488
StyI CCWWGG 2 cut(s) 1399, 1432
TaaI ACNGT 7 cut(s) 154, 386, 514, 766, 877, 898, 1264
TaiI ACGT 1 cut(s) 1382
TaqI TCGA 3 cut(s) 90, 493, 974
TauI GCSGC 4 cut(s) 50, 1983, 2314, 2344
TfiI GAWTC 5 cut(s) 835, 976, 1763, 2317, 2377
Tru1I TTAA 5 cut(s) 125, 704, 1044, 1119, 2022
Tru9I TTAA 5 cut(s) 125, 704, 1044, 1119, 2022
TscAI CASTG 7 cut(s) 124, 171, 389, 519, 769, 793, 1066
TseFI GTSAC 2 cut(s) 784, 2202
Tsp45I GTSAC 2 cut(s) 784, 2202
TspDTI ATGAA 6 cut(s) 180, 510, 1442, 1490, 1841, 2491
TspGWI ACGGA 2 cut(s) 229, 475
TspMI CCCGGG 1 cut(s) 1345
TspRI CASTG 7 cut(s) 124, 171, 389, 519, 769, 793, 1066
Van91I CCANNNNNTGG 2 cut(s) 788, 1553
Vha464I CTTAAG 1 cut(s) 703
VneI GTGCAC 1 cut(s) 1311
VpaK11BI GGWCC 6 cut(s) 56, 824, 857, 1088, 1349, 1819
XagI CCTNNNNNAGG 2 cut(s) 345, 1545
XapI RAATTY 5 cut(s) 679, 721, 1467, 1687, 2031
XceI RCATGY 2 cut(s) 696, 2488
XhoI CTCGAG 1 cut(s) 89
XmaI CCCGGG 1 cut(s) 1345
XmnI GAANNNNTTC 4 cut(s) 653, 1291, 1441, 1750
XspI CTAG 2 cut(s) 891, 921
Zsp2I ATGCAT 1 cut(s) 1209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.