MD16G1046800.v1.1

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0005569
Physical Location & Seq
Reverse (-)
3240515 .. 3249120
8606 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1046800.v1.1.491

Sequence Viewer

Length: 2025 bp
ATGGCGGAGAAGCAGCTGATCGTGGCCGTTGAAGGCACCGCCGCCATGGGGCCCTGCTGGGCAACCGTCGTCTCCGATTACCTTGATAAGATCATCAGGAGTTTCTGTGGAAACGAGCCAAGTGGGCAGAAGCTGTCGACTTCTACTGTTGAGCTCTCCCTGGTCACGTTCAATACTCATGGATCTTATTGTGGTTGCCTGGTACAACGGAGTGGCTGGACAAGGGATGTGGATCTCTTCTTACATAGGCTTTCAGCTATACCCTTTTCTGGGGGTGGTTTTAGTGATGCTGCAATTGCGGAAGGGCTTTCGGAAGCTCTAATGATGTTCCCTACAGTTCAAAATGGAAGCCAAAATCAACAAAATGTGGATTGTCAGAAGCATTGTGTTCTTATTGCTGCAAGTAAACCTCACCCTCTGCCCACACCAGTGTATCGGCCACAAATTCAAAATTTGGAGCAAAATGAAAATATTGATGCACAAACAGAAAATCGTCTTTATGATGCTGAGGCTGTTGCTAAATCATTTCCACAGAGCTCTGTTTCACTGTCAGTCATTTGTCCAATACAGCTACCTGAGCTCAGAGCAATATACAATGCGGGAAAGCGCAACCCTAGAGCGGCAGATCCACCAATTGATAATGCGAAAAATCCCCGCTTTCTTGTTTTAATCTCAGAGAATTTTATGGAGGCCCGTGCTACTTTAAGTCGTCCAGGAATTACAACTTTGCCCTCAAATCAGAGTCCTGTAAAAATGGACATAGCTCCTGTTGCTTCAGTTACTGGGCCGCCTCCAACTTCTATGCCCTCTGTGAATGGACCTGTTATGAATCGGCAACCAATTTCTGTTGGAAATGTGCCTCCTGCTACTGTAAAAGTTGAGCCAAGTACCGTAAGTTCCATGGGACCTTCTTTTGCACACATTCCATCTGTCCGTCCTCCTTCCCAAGGAGTCCCGAGCTTGCAGACTTCTTCACCATCTTCTGCTTCTCAAGAGATGGCCACAAACAATGATAATGTGCCAGATTTGAAACCTGTAGTGAGTGGTGTGACACATCCCTCGCGTCCTGTAGGTAGCATCCTCAATAACATCTCTCAGGCACGTGTGATGAACGCAGCAGCCTTAACTGGAGGAACTTCTATGGGACTCCCAACTATGGGTCAAAATCCTATGGCCATGCATGTATCAAATATGATATCTAGTGGAATGGCATCTTCAGTTGGTGCTGCTCAAAATGTATTTACATCTTCAGGGTCCGGCACTCTTACACAGGTTGCGCAGAACCCAGGTCTTAATTCATTCACTTCAGGTACCTCTAATGTCTCTGGCAACAATAACCTTGGGATGTCACAACCAATGAGCAACCTCCAAGGTGGTGTTAGTATGGGTCAAGCTGTGCCTGGTATGAGCCAAGGAAATCTCTCGGGACCGCAAATGGTTCAAAGTGGAATTGGCATGAACCCGAACATGATGAGTGCCCTTGGTTCAACAGGTGCCTCTTCTGGAACTGGTACAATGATTCCTACTCCAGGAATGGCTCAACAGGTGCAATCTGGGATGCAGTCACTTGGTGCAAACAATAATTCAGCATCTAATGTGCCGTTTTCACAACAGACATCAAGTGCTTTGGCATCTGCACAATCCAAATATGTGAAAGTCTGGGAGGGAAATTTATCTGGGCAGAGACAAGGGCAGCCTGTGTTTATCACTAGATTGGAGGGTTACAGGAGTGCTTCAGCTTCTGAGACGCTTGCAGCTAACTGGCCACCAACAATGCAAATAGTGAGGCTCATATCTCAGGACCACATGAATAACAAGCAATATGTTGGAAAGGCAGACTTTTTAGTTTTTCGGGCAATGAATCAGCATGGGTTTCTTGGGCAATTGCAAGAGAAGAAACTTTGTGCAGTGATCCAATTGCCCTCTCAGACACTACTGCTCTCTGTTTCAGACAAAGCATGCCGCTTGATTGGAATGCTGTTTCCAGGGGTGAGCTTGATATGGTTGTGTTTAAGCCACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med25_VWA

Protein Analysis

675

Amino Acids

70.92

Weight (kDa)

8.75

Isoelectric Point (pI)

45.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med25_VWA PF11265 3 - 226 8.3e-42 Mediator complex subunit 25 von Willebrand factor type A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1278
Acc65I GGTACC 1 cut(s) 1310
AccB1I GGYRCC 3 cut(s) 35, 1310, 1493
AccBSI CCGCTC 1 cut(s) 620
AccI GTMKAC 1 cut(s) 137
AccII CGCG 1 cut(s) 1063
AclWI GGATC 4 cut(s) 190, 240, 620, 1906
AcoI YGGCCR 5 cut(s) 24, 437, 999, 1173, 1763
AcsI RAATTY 4 cut(s) 444, 451, 679, 1669
AcuI CTGAAG 5 cut(s) 759, 1200, 1233, 1290, 1719
AcvI CACGTG 1 cut(s) 1103
AdeI CACNNNGTG 1 cut(s) 1571
AfaI GTAC 4 cut(s) 204, 889, 1312, 1513
AfiI CCNNNNNNNGG 8 cut(s) 48, 269, 270, 619, 947, 1156, 1157, 1529
AflIII ACRYGT 1 cut(s) 1102
AgsI TTSAA 7 cut(s) 32, 172, 341, 449, 1030, 1442, 1488
AjnI CCWGG 7 cut(s) 159, 198, 712, 1285, 1399, 1528, 1984
AleI CACNNNNGTG 1 cut(s) 428
Alw21I GWGCWC 3 cut(s) 156, 539, 582
Alw26I GTCTC 4 cut(s) 76, 1327, 1678, 1739
AlwI GGATC 4 cut(s) 190, 240, 620, 1906
AlwNI CAGNNNCTG 3 cut(s) 133, 782, 1742
Ama87I CYCGRG 2 cut(s) 955, 1423
AoxI GGCC 8 cut(s) 24, 50, 437, 690, 785, 999, 1173, 1763
ApaI GGGCCC 1 cut(s) 54
ApeKI GCWGC 8 cut(s) 13, 290, 398, 1115, 1118, 1226, 1693, 1754
ApoI RAATTY 4 cut(s) 444, 451, 679, 1669
Asp718I GGTACC 1 cut(s) 1310
AspLEI GCGC 2 cut(s) 609, 1279
AspS9I GGNCC 9 cut(s) 50, 51, 691, 785, 818, 905, 1254, 1427, 1801
AsuHPI GGTGA 3 cut(s) 404, 966, 2002
AvaI CYCGRG 2 cut(s) 955, 1423
AvaII GGWCC 5 cut(s) 818, 905, 1254, 1427, 1801
BaeGI GKGCMC 2 cut(s) 54, 1480
BaeI ACNNNNGTAYC 2 cut(s) 416, 449
BalI TGGCCA 3 cut(s) 1001, 1175, 1765
BanI GGYRCC 3 cut(s) 35, 1310, 1493
BanII GRGCYC 4 cut(s) 54, 156, 539, 582
BbrPI CACGTG 1 cut(s) 1103
Bbv12I GWGCWC 3 cut(s) 156, 539, 582
BbvCI CCTCAGC 1 cut(s) 507
BbvI GCAGC 8 cut(s) 25, 277, 385, 1127, 1130, 1213, 1705, 1766
BccI CCATC 3 cut(s) 934, 985, 991
BceAI ACGGC 2 cut(s) 11, 1585
BciT130I CCWGG 7 cut(s) 161, 200, 714, 1287, 1401, 1530, 1986
BcoDI GTCTC 4 cut(s) 76, 1327, 1678, 1739
BfaI CTAG 3 cut(s) 615, 1200, 1710
BfmI CTRYAG 3 cut(s) 333, 1035, 1068
BglI GCCNNNNNGGC 1 cut(s) 124
Bme1390I CCNGG 7 cut(s) 161, 200, 714, 1287, 1401, 1530, 1986
Bme18I GGWCC 5 cut(s) 818, 905, 1254, 1427, 1801
BmeT110I CYCGRG 2 cut(s) 955, 1423
BmgT120I GGNCC 9 cut(s) 50, 51, 691, 785, 818, 905, 1254, 1427, 1801
BmiI GGNNCC 8 cut(s) 37, 51, 52, 906, 1255, 1312, 1428, 1495
BmrFI CCNGG 7 cut(s) 161, 200, 714, 1287, 1401, 1530, 1986
BmrI ACTGGG 1 cut(s) 792
BmsI GCATC 8 cut(s) 277, 466, 493, 1086, 1220, 1548, 1598, 1640
BmuI ACTGGG 1 cut(s) 792
BpmI CTGGAG 2 cut(s) 1149, 1512
Bpu10I CCTNAGC 2 cut(s) 507, 576
BpuEI CTTGAG 1 cut(s) 975
BsaAI YACGTR 1 cut(s) 1103
BsaBI GATNNNNATC 1 cut(s) 231
BsaXI ACNNNNNCTCC 2 cut(s) 91, 121
Bsc4I CCNNNNNNNGG 8 cut(s) 48, 269, 270, 619, 947, 1156, 1157, 1529
Bse1I ACTGG 5 cut(s) 428, 787, 1132, 1513, 1766
Bse3DI GCAATG 1 cut(s) 1863
Bse8I GATNNNNATC 1 cut(s) 231
BseBI CCWGG 7 cut(s) 161, 200, 714, 1287, 1401, 1530, 1986
BseGI GGATG 5 cut(s) 232, 1054, 1077, 1350, 1563
BseJI GATNNNNATC 1 cut(s) 231
BseLI CCNNNNNNNGG 8 cut(s) 48, 269, 270, 619, 947, 1156, 1157, 1529
BseMI GCAATG 1 cut(s) 1863
BseMII CTCAG 8 cut(s) 498, 567, 595, 687, 1109, 1734, 1811, 1940
BseNI ACTGG 5 cut(s) 428, 787, 1132, 1513, 1766
BseSI GKGCMC 2 cut(s) 54, 1480
BseXI GCAGC 8 cut(s) 25, 277, 385, 1127, 1130, 1213, 1705, 1766
BseYI CCCAGC 1 cut(s) 57
BsgI GTGCAG 2 cut(s) 1620, 1926
Bsh1236I CGCG 1 cut(s) 1063
BshFI GGCC 8 cut(s) 26, 52, 439, 692, 787, 1001, 1175, 1765
BshNI GGYRCC 3 cut(s) 35, 1310, 1493
BsiHKAI GWGCWC 3 cut(s) 156, 539, 582
BsiHKCI CYCGRG 2 cut(s) 955, 1423
BsiSI CCGG 1 cut(s) 1257
BslFI GGGAC 4 cut(s) 918, 938, 1158, 1440
BslI CCNNNNNNNGG 8 cut(s) 48, 269, 270, 619, 947, 1156, 1157, 1529
BsmAI GTCTC 4 cut(s) 76, 1327, 1678, 1739
BsmBI CGTCTC 2 cut(s) 76, 1739
BsmFI GGGAC 4 cut(s) 918, 938, 1158, 1440
BsmI GAATGC 1 cut(s) 1980
BsnI GGCC 8 cut(s) 26, 52, 439, 692, 787, 1001, 1175, 1765
BsoBI CYCGRG 2 cut(s) 955, 1423
Bsp120I GGGCCC 1 cut(s) 50
Bsp1286I GDGCHC 5 cut(s) 54, 156, 539, 582, 1480
Bsp143I GATC 6 cut(s) 18, 90, 182, 232, 625, 1911
Bsp19I CCATGG 2 cut(s) 45, 900
BspANI GGCC 8 cut(s) 26, 52, 439, 692, 787, 1001, 1175, 1765
BspCNI CTCAG 8 cut(s) 499, 568, 594, 686, 1108, 1735, 1810, 1939
BspFNI CGCG 1 cut(s) 1063
BspLI GGNNCC 8 cut(s) 37, 51, 52, 906, 1255, 1312, 1428, 1495
BspPI GGATC 4 cut(s) 190, 240, 620, 1906
BspT107I GGYRCC 3 cut(s) 35, 1310, 1493
BsrBI CCGCTC 1 cut(s) 620
BsrDI GCAATG 1 cut(s) 1863
BsrI ACTGG 5 cut(s) 428, 787, 1132, 1513, 1766
BssMI GATC 6 cut(s) 18, 90, 182, 232, 625, 1911
BssT1I CCWWGG 7 cut(s) 45, 900, 946, 1339, 1369, 1411, 1480
Bst2UI CCWGG 7 cut(s) 161, 200, 714, 1287, 1401, 1530, 1986
Bst4CI ACNGT 6 cut(s) 67, 148, 337, 549, 871, 892
Bst6I CTCTTC 2 cut(s) 242, 1504
BstBAI YACGTR 1 cut(s) 1103
BstC8I GCNNGC 3 cut(s) 962, 1752, 1960
BstDEI CTNAG 8 cut(s) 507, 576, 581, 673, 1095, 1743, 1797, 1926
BstDSI CCRYGG 2 cut(s) 45, 900
BstENI CCTNNNNNAGG 2 cut(s) 945, 1527
BstF5I GGATG 5 cut(s) 232, 1054, 1077, 1350, 1563
BstFNI CGCG 1 cut(s) 1063
BstHHI GCGC 2 cut(s) 609, 1279
BstKTI GATC 6 cut(s) 21, 93, 185, 235, 628, 1914
BstMAI GTCTC 4 cut(s) 76, 1327, 1678, 1739
BstMBI GATC 6 cut(s) 18, 90, 182, 232, 625, 1911
BstMWI GCNNNNNNNGC 4 cut(s) 124, 296, 577, 770
BstNI CCWGG 7 cut(s) 161, 200, 714, 1287, 1401, 1530, 1986
BstNSI RCATGY 2 cut(s) 1184, 1962
BstSCI CCNGG 7 cut(s) 159, 198, 712, 1285, 1399, 1528, 1984
BstSFI CTRYAG 3 cut(s) 333, 1035, 1068
BstSLI GKGCMC 2 cut(s) 54, 1480
BstUI CGCG 1 cut(s) 1063
BstV1I GCAGC 8 cut(s) 25, 277, 385, 1127, 1130, 1213, 1705, 1766
BstX2I RGATCY 3 cut(s) 182, 232, 625
BstYI RGATCY 3 cut(s) 182, 232, 625
BsuRI GGCC 8 cut(s) 26, 52, 439, 692, 787, 1001, 1175, 1765
BtgI CCRYGG 2 cut(s) 45, 900
BtsCI GGATG 5 cut(s) 232, 1054, 1077, 1350, 1563
BtsI GCAGTG 1 cut(s) 1914
BtsIMutI CAGTG 3 cut(s) 435, 545, 1914
Cac8I GCNNGC 3 cut(s) 962, 1752, 1960
CaiI CAGNNNCTG 3 cut(s) 133, 782, 1742
CfoI GCGC 2 cut(s) 609, 1279
Cfr13I GGNCC 9 cut(s) 50, 51, 691, 785, 818, 905, 1254, 1427, 1801
CseI GACGC 2 cut(s) 1052, 1756
Csp6I GTAC 4 cut(s) 203, 888, 1311, 1512
CspCI CAANNNNNGTGG 2 cut(s) 210, 245
CviQI GTAC 4 cut(s) 203, 888, 1311, 1512
DdeI CTNAG 8 cut(s) 507, 576, 581, 673, 1095, 1743, 1797, 1926
DpnI GATC 6 cut(s) 20, 92, 184, 234, 627, 1913
DpnII GATC 6 cut(s) 18, 90, 182, 232, 625, 1911
DraIII CACNNNGTG 1 cut(s) 1571
EaeI YGGCCR 5 cut(s) 24, 437, 999, 1173, 1763
Eam1104I CTCTTC 2 cut(s) 242, 1504
EarI CTCTTC 2 cut(s) 242, 1504
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 3 cut(s) 154, 537, 580
Eco130I CCWWGG 7 cut(s) 45, 900, 946, 1339, 1369, 1411, 1480
Eco24I GRGCYC 4 cut(s) 54, 156, 539, 582
Eco32I GATATC 1 cut(s) 1197
Eco47I GGWCC 5 cut(s) 818, 905, 1254, 1427, 1801
Eco53kI GAGCTC 3 cut(s) 154, 537, 580
Eco57I CTGAAG 5 cut(s) 759, 1200, 1233, 1290, 1719
Eco72I CACGTG 1 cut(s) 1103
Eco88I CYCGRG 2 cut(s) 955, 1423
EcoICRI GAGCTC 3 cut(s) 154, 537, 580
EcoNI CCTNNNNNAGG 2 cut(s) 945, 1527
EcoO109I RGGNCCY 3 cut(s) 50, 51, 905
EcoRII CCWGG 7 cut(s) 159, 198, 712, 1285, 1399, 1528, 1984
EcoRV GATATC 1 cut(s) 1197
EcoT14I CCWWGG 7 cut(s) 45, 900, 946, 1339, 1369, 1411, 1480
EcoT22I ATGCAT 1 cut(s) 1182
EcoT38I GRGCYC 4 cut(s) 54, 156, 539, 582
ErhI CCWWGG 7 cut(s) 45, 900, 946, 1339, 1369, 1411, 1480
Esp3I CGTCTC 2 cut(s) 76, 1739
FalI AAGNNNNNCTT 2 cut(s) 1823, 1855
FaqI GGGAC 4 cut(s) 918, 938, 1158, 1440
FauI CCCGC 2 cut(s) 592, 662
FblI GTMKAC 1 cut(s) 137
FokI GGATG 5 cut(s) 239, 1041, 1064, 1357, 1570
FriOI GRGCYC 4 cut(s) 54, 156, 539, 582
FspBI CTAG 3 cut(s) 615, 1200, 1710
FspI TGCGCA 1 cut(s) 1278
GlaI GCGC 2 cut(s) 608, 1278
GsaI CCCAGC 1 cut(s) 61
GsuI CTGGAG 2 cut(s) 1149, 1512
HaeIII GGCC 8 cut(s) 26, 52, 439, 692, 787, 1001, 1175, 1765
HapII CCGG 1 cut(s) 1257
HgaI GACGC 2 cut(s) 1052, 1756
HhaI GCGC 2 cut(s) 609, 1279
Hin6I GCGC 2 cut(s) 607, 1277
HinP1I GCGC 2 cut(s) 607, 1277
HincII GTYRAC 1 cut(s) 138
HindII GTYRAC 1 cut(s) 138
HinfI GANTC 6 cut(s) 742, 829, 951, 1146, 1519, 1861
HpaII CCGG 1 cut(s) 1257
HphI GGTGA 3 cut(s) 404, 966, 2002
Hpy166II GTNNAC 2 cut(s) 138, 407
Hpy188I TCNGA 9 cut(s) 76, 313, 378, 584, 676, 741, 1744, 1929, 1951
Hpy188III TCNNGA 6 cut(s) 97, 955, 992, 1425, 1503, 1799
Hpy8I GTNNAC 2 cut(s) 138, 407
Hpy99I CGWCG 1 cut(s) 71
HpyAV CCTTC 4 cut(s) 26, 296, 918, 951
HpyCH4III ACNGT 6 cut(s) 67, 148, 337, 549, 871, 892
HpyCH4IV ACGT 2 cut(s) 167, 1102
HpyF10VI GCNNNNNNNGC 4 cut(s) 124, 296, 577, 770
HpyF3I CTNAG 8 cut(s) 507, 576, 581, 673, 1095, 1743, 1797, 1926
HpySE526I ACGT 2 cut(s) 167, 1102
HspAI GCGC 2 cut(s) 607, 1277
KpnI GGTACC 1 cut(s) 1314
Kzo9I GATC 6 cut(s) 18, 90, 182, 232, 625, 1911
LmnI GCTCC 2 cut(s) 457, 769
Lsp1109I GCAGC 8 cut(s) 25, 277, 385, 1127, 1130, 1213, 1705, 1766
LweI GCATC 8 cut(s) 277, 466, 493, 1086, 1220, 1548, 1598, 1640
MaeI CTAG 3 cut(s) 615, 1200, 1710
MaeII ACGT 2 cut(s) 167, 1102
MaeIII GTNAC 6 cut(s) 163, 778, 1048, 1347, 1563, 1721
MalI GATC 6 cut(s) 20, 92, 184, 234, 627, 1913
MbiI CCGCTC 1 cut(s) 620
MboI GATC 6 cut(s) 18, 90, 182, 232, 625, 1911
MboII GAAGA 7 cut(s) 229, 963, 972, 1206, 1239, 1491, 1906
MfeI CAATTG 4 cut(s) 294, 633, 1883, 1916
MflI RGATCY 3 cut(s) 182, 232, 625
MhlI GDGCHC 5 cut(s) 54, 156, 539, 582, 1480
MlsI TGGCCA 3 cut(s) 1001, 1175, 1765
MluNI TGGCCA 3 cut(s) 1001, 1175, 1765
MlyI GAGTC 3 cut(s) 751, 960, 1140
MmeI TCCRAC 3 cut(s) 818, 829, 1807
Mox20I TGGCCA 3 cut(s) 1001, 1175, 1765
Mph1103I ATGCAT 1 cut(s) 1182
MscI TGGCCA 3 cut(s) 1001, 1175, 1765
MseI TTAA 5 cut(s) 668, 704, 1124, 1293, 2012
MslI CAYNNNNRTG 1 cut(s) 428
Msp20I TGGCCA 3 cut(s) 1001, 1175, 1765
MspA1I CMGCKG 1 cut(s) 16
MspI CCGG 1 cut(s) 1257
MspR9I CCNGG 7 cut(s) 161, 200, 714, 1287, 1401, 1530, 1986
MunI CAATTG 4 cut(s) 294, 633, 1883, 1916
Mva1269I GAATGC 1 cut(s) 1980
MvaI CCWGG 7 cut(s) 161, 200, 714, 1287, 1401, 1530, 1986
MvnI CGCG 1 cut(s) 1063
MwoI GCNNNNNNNGC 4 cut(s) 124, 296, 577, 770
NcoI CCATGG 2 cut(s) 45, 900
NdeII GATC 6 cut(s) 18, 90, 182, 232, 625, 1911
NlaIV GGNNCC 8 cut(s) 37, 51, 52, 906, 1255, 1312, 1428, 1495
NmuCI GTSAC 4 cut(s) 163, 1048, 1347, 1563
NsbI TGCGCA 1 cut(s) 1278
NsiI ATGCAT 1 cut(s) 1182
NspI RCATGY 2 cut(s) 1184, 1962
OliI CACNNNNGTG 1 cut(s) 428
PaeI GCATGC 1 cut(s) 1962
PctI GAATGC 1 cut(s) 1980
PfeI GAWTC 3 cut(s) 829, 1519, 1861
PfoI TCCNGGA 2 cut(s) 712, 1528
PleI GAGTC 3 cut(s) 750, 959, 1140
PmaCI CACGTG 1 cut(s) 1103
PmlI CACGTG 1 cut(s) 1103
PpsI GAGTC 3 cut(s) 750, 959, 1140
Ppu21I YACGTR 1 cut(s) 1103
PpuMI RGGWCCY 1 cut(s) 905
Psp124BI GAGCTC 3 cut(s) 156, 539, 582
Psp5II RGGWCCY 1 cut(s) 905
Psp6I CCWGG 7 cut(s) 159, 198, 712, 1285, 1399, 1528, 1984
PspCI CACGTG 1 cut(s) 1103
PspFI CCCAGC 1 cut(s) 57
PspGI CCWGG 7 cut(s) 159, 198, 712, 1285, 1399, 1528, 1984
PspN4I GGNNCC 8 cut(s) 37, 51, 52, 906, 1255, 1312, 1428, 1495
PspOMI GGGCCC 1 cut(s) 50
PspPI GGNCC 9 cut(s) 50, 51, 691, 785, 818, 905, 1254, 1427, 1801
PspPPI RGGWCCY 1 cut(s) 905
PsrI GAACNNNNNNTAC 2 cut(s) 880, 912
PstNI CAGNNNCTG 3 cut(s) 133, 782, 1742
PsuI RGATCY 3 cut(s) 182, 232, 625
PvuII CAGCTG 1 cut(s) 16
RsaI GTAC 4 cut(s) 204, 889, 1312, 1513
RsaNI GTAC 4 cut(s) 203, 888, 1311, 1512
RseI CAYNNNNRTG 1 cut(s) 428
SacI GAGCTC 3 cut(s) 156, 539, 582
SalI GTCGAC 1 cut(s) 136
SaqAI TTAA 5 cut(s) 668, 704, 1124, 1293, 2012
Sau3AI GATC 6 cut(s) 18, 90, 182, 232, 625, 1911
Sau96I GGNCC 9 cut(s) 50, 51, 691, 785, 818, 905, 1254, 1427, 1801
SchI GAGTC 3 cut(s) 751, 960, 1140
ScrFI CCNGG 7 cut(s) 161, 200, 714, 1287, 1401, 1530, 1986
SduI GDGCHC 5 cut(s) 54, 156, 539, 582, 1480
SfaNI GCATC 8 cut(s) 277, 466, 493, 1086, 1220, 1548, 1598, 1640
SfcI CTRYAG 3 cut(s) 333, 1035, 1068
SinI GGWCC 5 cut(s) 818, 905, 1254, 1427, 1801
SmiMI CAYNNNNRTG 1 cut(s) 428
SmlI CTYRAG 1 cut(s) 990
SmoI CTYRAG 1 cut(s) 990
SphI GCATGC 1 cut(s) 1962
SspI AATATT 1 cut(s) 472
SspMI CTAG 3 cut(s) 615, 1200, 1710
SstI GAGCTC 3 cut(s) 156, 539, 582
StyD4I CCNGG 7 cut(s) 159, 198, 712, 1285, 1399, 1528, 1984
StyI CCWWGG 7 cut(s) 45, 900, 946, 1339, 1369, 1411, 1480
TaaI ACNGT 6 cut(s) 67, 148, 337, 549, 871, 892
TaiI ACGT 2 cut(s) 170, 1105
TaqI TCGA 1 cut(s) 137
TauI GCSGC 4 cut(s) 44, 623, 790, 1965
TfiI GAWTC 3 cut(s) 829, 1519, 1861
Tru1I TTAA 5 cut(s) 668, 704, 1124, 1293, 2012
Tru9I TTAA 5 cut(s) 668, 704, 1124, 1293, 2012
TscAI CASTG 3 cut(s) 435, 552, 1914
TseFI GTSAC 4 cut(s) 163, 1048, 1347, 1563
TseI GCWGC 8 cut(s) 13, 290, 398, 1115, 1118, 1226, 1693, 1754
Tsp45I GTSAC 4 cut(s) 163, 1048, 1347, 1563
TspDTI ATGAA 7 cut(s) 480, 842, 1124, 1287, 1472, 1823, 1874
TspGWI ACGGA 2 cut(s) 223, 923
TspRI CASTG 3 cut(s) 435, 552, 1914
VpaK11BI GGWCC 5 cut(s) 818, 905, 1254, 1427, 1801
XagI CCTNNNNNAGG 2 cut(s) 945, 1527
XapI RAATTY 4 cut(s) 444, 451, 679, 1669
XceI RCATGY 2 cut(s) 1184, 1962
XmiI GTMKAC 1 cut(s) 137
XspI CTAG 3 cut(s) 615, 1200, 1710
Zsp2I ATGCAT 1 cut(s) 1182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.