FvH4_4g27961

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0005569
Physical Location & Seq
Reverse (-)
28753564 .. 28762942
9379 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27961.t8

Sequence Viewer

Length: 2589 bp
ATGGCGGAGAAGCAGCTGATCGTCGTCGTCGAAGGCACGGCGGCCATGGGGCCTTACTGGCCCGCCGTCGTCTCCGATTACGTCGAGAAGGTCATCAGGTCTTTATCTGGAAATGAGTTAAGTGGTCAGAAGCCCTCCTCTTCTACTGTTGAGATGTCTCTCGTCACATTCAATACTCATGGATCTTATTCTGGTTGCTTGGTACAACGGACTGGTTGGACAAGAGATATTGAACTCTTCTTACAATGGCTATCAGCTATTCCTCTAGCAGGTGGTGGTTTTAGTGATGCTGCAATTGCAGAAGGGCTTTCTGAAGCCTTAATGATGTTCCCTACCATTCAAAATGGAGGCCAAAATCAACAAAATGTGGATTTTCAAAAGCATTGCATTCTTGTTGCTGCTAGTAACCCTCATCCCCTACCCACACCAGTCTATAGACCACAAATGCAAAATGTGGAGCAGAGTGAAAATTTTGATGCACAAACTGAAAGCCGTCTGTATGATGCTGAGGCTGTTGCTAAATCATTTCCACAGTGCTCTGTGTCTCTGTCAGTCATCTGTCCAAAACAGCTTCCTAAACTCAGAGCTATTTACAATGCGGGAAAGCGCAACCCCCGGGCAGAAGATCCGCCGATTGATAATGTGAAAAACCCCCACTTTCTCGTTCTAATCTCAGAGAATTTTATTGAGGCCCGGGCTACTTTAAGTCGTCCTGGAAGTACAACTTTGCCCTCAATTCCAAGTCCTGTAAAGATGGATATAGCTCCTGTTGCATCAGTAACAGGGCCACCTCCAACTTCTATGCCATCTGTGAATGGATCTGTTATGAATCGCCCATCATTTTCTGTTGGAAATGTGCCTCCTGCTACAGTAAAAGTGGAGCCAAGCACTGTAAGTTCAATGGTAGCTGGACCTGCTTTTGCACATGTTCCATCTGTTCGTCCTCCTTCCCAAGGAGTTCCAACCTTGCAAACTTCTTCACCATCTTCTGGTGAGATGAATACAAATAATGAGAGTGTGCCAGATTTGAAACCTATAGTGAGTGGTGTGACACATCAACTACGTCCGGTGGGCAGCATCCTCAATAATATCTCTCAAGCACGAGTGATGAATTCTGCAGCCTTATCTGGAGGAACTTCTATAGGTCTCCAAAATATGGGTCAAAATCCAATGGCCATGCATGTATCAAACATGATATCGAGTGGAATGGCATCTTCAGTGGCTGCTGCCCAAAACGTATTTTCATCTTCAGCGCCTCACACTCTTACACAGGTTCCTCAGAGCTCAGGTCTTAATACATTCACTTCAGCTACTTCCAATGTGTCTGGAAATTCTAACCTTGGCATGTCGCAACCAATGAGTAACCTCCAAGGTGGTGTTAGTATTGGGCAATCTGTTCCTGGCATGAACCAAAATCTCTCAGGACCCCAAATGGTTCAAAATGGCATTGGAATGAATCCAAACATGATGAGTGGCCATGGTTCAGCAGGTTCGTCCTCTGGAACTGGTACAATGATTCCTACTCCTGGAATCTCTCAACAGGCCCAATCTGGGATACAGTCACTTGGTGCAAACAATAGTTCAATATCTAATCCGTTGTCACAACAGACATCAAGTGCTCTGCAATCTGCACAATCCAAATATGTAAAAGTCTGGGAGGGAAATTTATCTGGGCAGAGACAAGGACAACCTGTGTTTATTACTAGATTGGAAGGATACAGAAGTGCTTCAGCATCTGAGACGCTTGCAGCAAACTGGCCACCGACAATGCAAATAGTCAGGCTTATATCTCAGGATCACATGAATAACAAGCAATATGTGGGAAGGGCCGACTTTCTAGTATTTCGGGCAATGAATCAGCATGGATTTCTTGGGCAGTTGCAAGAAAAGAAGCTTTGTGCTGTCATCCAATTGCCGTCTCAGACACTACTGCTATCTGTTTCGGACAAAGCTTGCCGCTTGATTGGGATGCTTTTCCCAGGGCTAAGTCTACCATCGGTTCAGGATATGGTCGTGTTTAAGCCACAAGTAGGTGGTCAGCAACAAATGCAACAAATGCAACAACAGCAACCTCAGCTACAACAAATGCAACAACAGCAGCCGCAGCTACAACAGCTGCAGCAACAGCAGCCTCAGCTACAACAAATGCAGCAACAACAGCCGCAGCTACAACAAATGCAGCAACAGCAGCCGCAGCTACAACAGATGCAGCAACAGCAACCACAGCTACAGCAGCAACAATTGCAACCACAGCTACAGCAACAACAGCAACCCCAACTGCAGCAGCAGCATCCACAGATGCAACAACAACAACAACAACAACAGCAACAGCAGCAGCAGCATCCGCAGATGCAACAACAGCAGCAACATCCTCAGATGCAGCAACCACAGCAGCAGCTGTCGCAGCTCCAGTCGCAACAGCAAATTCCACAACTGCAGCAACTACAGCAACAACATCAACATCAACAACAACAGTTGGTTGGGGGTGGAATGGGTCAAGCTTATGGTCAAGGCCGGTCACAATTAGTTTCACAAGGACAAGTTTCATCACAGCAAGGGCCTAATATGCCAGGAGGCTTTATGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med25_VWA

Protein Analysis

863

Amino Acids

93.31

Weight (kDa)

8.8

Isoelectric Point (pI)

66.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med25_VWA PF11265 5 - 119 1.3e-13 Mediator complex subunit 25 von Willebrand factor type A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 260
Acc36I ACCTGC 3 cut(s) 260, 922, 1478
AccB7I CCANNNNNTGG 2 cut(s) 989, 1156
AccI GTMKAC 1 cut(s) 1990
AclWI GGATC 4 cut(s) 190, 620, 826, 1803
AcoI YGGCCR 4 cut(s) 42, 1173, 1474, 1757
AcsI RAATTY 6 cut(s) 469, 679, 1111, 1330, 1663, 2424
AcuI CTGAAG 5 cut(s) 333, 1200, 1233, 1290, 1713
AdeI CACNNNGTG 1 cut(s) 1568
AfaI GTAC 3 cut(s) 204, 721, 1510
AfiI CCNNNNNNNGG 7 cut(s) 269, 953, 989, 1156, 1526, 1551, 2030
AflIII ACRYGT 1 cut(s) 925
AgsI TTSAA 8 cut(s) 172, 233, 341, 377, 900, 1030, 1439, 1584
AjnI CCWGG 5 cut(s) 712, 1399, 1525, 1978, 2569
Alw21I GWGCWC 3 cut(s) 539, 1286, 1621
Alw26I GTCTC 7 cut(s) 76, 162, 549, 1151, 1672, 1733, 1923
AlwI GGATC 4 cut(s) 190, 620, 826, 1803
AlwNI CAGNNNCTG 3 cut(s) 1223, 1736, 2398
Ama87I CYCGRG 2 cut(s) 615, 693
ApoI RAATTY 6 cut(s) 469, 679, 1111, 1330, 1663, 2424
ArsI GACNNNNNNTTYG 2 cut(s) 1144, 1176
Asp700I GAANNNNTTC 1 cut(s) 1726
AspLEI GCGC 2 cut(s) 609, 1255
AspS9I GGNCC 9 cut(s) 50, 60, 691, 785, 911, 1424, 1543, 1827, 2558
AsuC2I CCSGG 4 cut(s) 616, 617, 694, 695
AsuHPI GGTGA 2 cut(s) 972, 1004
AvaI CYCGRG 2 cut(s) 615, 693
AvaII GGWCC 2 cut(s) 911, 1424
BaeI ACNNNNGTAYC 2 cut(s) 1708, 1741
BalI TGGCCA 3 cut(s) 1175, 1476, 1759
BanII GRGCYC 1 cut(s) 1286
BauI CACGAG 1 cut(s) 1101
Bbv12I GWGCWC 3 cut(s) 539, 1286, 1621
BbvCI CCTCAGC 3 cut(s) 507, 2073, 2133
BccI CCATC 6 cut(s) 748, 814, 844, 940, 991, 2002
BceAI ACGGC 4 cut(s) 50, 54, 477, 1900
BciT130I CCWGG 5 cut(s) 714, 1401, 1527, 1980, 2571
BciVI GTATCC 2 cut(s) 1548, 1709
BcnI CCSGG 4 cut(s) 616, 617, 694, 695
BcoDI GTCTC 7 cut(s) 76, 162, 549, 1151, 1672, 1733, 1923
BfaI CTAG 4 cut(s) 266, 402, 1704, 1838
BfoI RGCGCY 1 cut(s) 1256
BfuAI ACCTGC 3 cut(s) 260, 922, 1478
BfuI GTATCC 2 cut(s) 1548, 1709
BglI GCCNNNNNGGC 1 cut(s) 58
Bme1390I CCNGG 9 cut(s) 616, 617, 694, 695, 714, 1401, 1527, 1980, 2571
Bme18I GGWCC 2 cut(s) 911, 1424
BmeT110I CYCGRG 2 cut(s) 615, 693
BmgT120I GGNCC 9 cut(s) 50, 60, 691, 785, 911, 1424, 1543, 1827, 2558
BmiI GGNNCC 4 cut(s) 51, 882, 1275, 1426
BmrFI CCNGG 9 cut(s) 616, 617, 694, 695, 714, 1401, 1527, 1980, 2571
BpmI CTGGAG 2 cut(s) 1149, 2393
Bpu10I CCTNAGC 4 cut(s) 507, 1285, 2073, 2133
BpuEI CTTGAG 1 cut(s) 1080
BpuMI CCSGG 4 cut(s) 616, 617, 694, 695
BsaI GGTCTC 1 cut(s) 1151
BsaWI WCCGGW 1 cut(s) 1066
Bsc4I CCNNNNNNNGG 7 cut(s) 269, 953, 989, 1156, 1526, 1551, 2030
Bse118I RCCGGY 1 cut(s) 2514
Bse1I ACTGG 6 cut(s) 62, 217, 428, 1510, 1760, 2410
Bse3DI GCAATG 2 cut(s) 382, 1857
BseBI CCWGG 5 cut(s) 714, 1401, 1527, 1980, 2571
BseGI GGATG 7 cut(s) 412, 1077, 1905, 1974, 2290, 2341, 2368
BseLI CCNNNNNNNGG 7 cut(s) 269, 953, 989, 1156, 1526, 1551, 2030
BseMI GCAATG 2 cut(s) 382, 1857
BseNI ACTGG 6 cut(s) 62, 217, 428, 1510, 1760, 2410
BseRI GAGGAG 1 cut(s) 127
BsgI GTGCAG 1 cut(s) 1614
BsiHKAI GWGCWC 3 cut(s) 539, 1286, 1621
BsiHKCI CYCGRG 2 cut(s) 615, 693
BsiSI CCGG 4 cut(s) 616, 694, 1067, 2515
BslI CCNNNNNNNGG 7 cut(s) 269, 953, 989, 1156, 1526, 1551, 2030
BsmAI GTCTC 7 cut(s) 76, 162, 549, 1151, 1672, 1733, 1923
BsmBI CGTCTC 3 cut(s) 76, 1733, 1923
BsmI GAATGC 1 cut(s) 387
Bso31I GGTCTC 1 cut(s) 1151
BsoBI CYCGRG 2 cut(s) 615, 693
Bsp1286I GDGCHC 3 cut(s) 539, 1286, 1621
Bsp143I GATC 5 cut(s) 18, 182, 625, 818, 1795
Bsp19I CCATGG 2 cut(s) 45, 1477
BspLI GGNNCC 4 cut(s) 51, 882, 1275, 1426
BspMAI CTGCAG 4 cut(s) 1120, 2121, 2283, 2439
BspMI ACCTGC 3 cut(s) 260, 922, 1478
BspPI GGATC 4 cut(s) 190, 620, 826, 1803
BspTNI GGTCTC 1 cut(s) 1151
BsrDI GCAATG 2 cut(s) 382, 1857
BsrFI RCCGGY 1 cut(s) 2514
BsrI ACTGG 6 cut(s) 62, 217, 428, 1510, 1760, 2410
BssAI RCCGGY 1 cut(s) 2514
BssMI GATC 5 cut(s) 18, 182, 625, 818, 1795
BssSI CACGAG 1 cut(s) 1101
BssT1I CCWWGG 5 cut(s) 45, 952, 1339, 1369, 1477
Bst2BI CACGAG 1 cut(s) 1101
Bst2UI CCWGG 5 cut(s) 714, 1401, 1527, 1980, 2571
Bst4CI ACNGT 6 cut(s) 148, 534, 871, 892, 1560, 2475
Bst6I CTCTTC 2 cut(s) 145, 242
BstAPI GCANNNNNTGC 3 cut(s) 2047, 2056, 2242
BstC8I GCNNGC 3 cut(s) 63, 1746, 1954
BstDSI CCRYGG 2 cut(s) 45, 1477
BstENI CCTNNNNNAGG 2 cut(s) 267, 951
BstF5I GGATG 7 cut(s) 412, 1077, 1905, 1974, 2290, 2341, 2368
BstH2I RGCGCY 1 cut(s) 1256
BstHHI GCGC 2 cut(s) 609, 1255
BstKTI GATC 5 cut(s) 21, 185, 628, 821, 1798
BstMAI GTCTC 7 cut(s) 76, 162, 549, 1151, 1672, 1733, 1923
BstMBI GATC 5 cut(s) 18, 182, 625, 818, 1795
BstNI CCWGG 5 cut(s) 714, 1401, 1527, 1980, 2571
BstNSI RCATGY 3 cut(s) 929, 1184, 1348
BstSCI CCNGG 9 cut(s) 614, 615, 692, 693, 712, 1399, 1525, 1978, 2569
BstX2I RGATCY 3 cut(s) 182, 625, 818
BstYI RGATCY 3 cut(s) 182, 625, 818
BsuI GTATCC 2 cut(s) 1548, 1709
BtgI CCRYGG 2 cut(s) 45, 1477
BtsCI GGATG 7 cut(s) 412, 1077, 1905, 1974, 2290, 2341, 2368
BtsIMutI CAGTG 3 cut(s) 539, 888, 1224
BveI ACCTGC 3 cut(s) 260, 922, 1478
Cac8I GCNNGC 3 cut(s) 63, 1746, 1954
CaiI CAGNNNCTG 3 cut(s) 1223, 1736, 2398
CfoI GCGC 2 cut(s) 609, 1255
Cfr10I RCCGGY 1 cut(s) 2514
Cfr13I GGNCC 9 cut(s) 50, 60, 691, 785, 911, 1424, 1543, 1827, 2558
Cfr9I CCCGGG 2 cut(s) 615, 693
CseI GACGC 1 cut(s) 1750
Csp6I GTAC 3 cut(s) 203, 720, 1509
CviQI GTAC 3 cut(s) 203, 720, 1509
DpnI GATC 5 cut(s) 20, 184, 627, 820, 1797
DpnII GATC 5 cut(s) 18, 182, 625, 818, 1795
DraIII CACNNNGTG 1 cut(s) 1568
EaeI YGGCCR 4 cut(s) 42, 1173, 1474, 1757
Eam1104I CTCTTC 2 cut(s) 145, 242
EarI CTCTTC 2 cut(s) 145, 242
EciI GGCGGA 2 cut(s) 20, 618
Ecl136II GAGCTC 1 cut(s) 1284
Eco130I CCWWGG 5 cut(s) 45, 952, 1339, 1369, 1477
Eco24I GRGCYC 1 cut(s) 1286
Eco31I GGTCTC 1 cut(s) 1151
Eco32I GATATC 1 cut(s) 1197
Eco47I GGWCC 2 cut(s) 911, 1424
Eco53kI GAGCTC 1 cut(s) 1284
Eco57I CTGAAG 5 cut(s) 333, 1200, 1233, 1290, 1713
Eco88I CYCGRG 2 cut(s) 615, 693
EcoICRI GAGCTC 1 cut(s) 1284
EcoNI CCTNNNNNAGG 2 cut(s) 267, 951
EcoO109I RGGNCCY 3 cut(s) 50, 1424, 2558
EcoRI GAATTC 1 cut(s) 1111
EcoRII CCWGG 5 cut(s) 712, 1399, 1525, 1978, 2569
EcoRV GATATC 1 cut(s) 1197
EcoT14I CCWWGG 5 cut(s) 45, 952, 1339, 1369, 1477
EcoT22I ATGCAT 1 cut(s) 1182
EcoT38I GRGCYC 1 cut(s) 1286
ErhI CCWWGG 5 cut(s) 45, 952, 1339, 1369, 1477
Esp3I CGTCTC 3 cut(s) 76, 1733, 1923
FalI AAGNNNNNCTT 2 cut(s) 709, 741
FauI CCCGC 2 cut(s) 70, 592
FblI GTMKAC 1 cut(s) 1990
FokI GGATG 7 cut(s) 399, 1064, 1892, 1981, 2277, 2328, 2355
FriOI GRGCYC 1 cut(s) 1286
FspBI CTAG 4 cut(s) 266, 402, 1704, 1838
GlaI GCGC 2 cut(s) 608, 1254
GsuI CTGGAG 2 cut(s) 1149, 2393
HaeII RGCGCY 1 cut(s) 1256
HapII CCGG 4 cut(s) 616, 694, 1067, 2515
HgaI GACGC 1 cut(s) 1750
HhaI GCGC 2 cut(s) 609, 1255
Hin6I GCGC 2 cut(s) 607, 1253
HinP1I GCGC 2 cut(s) 607, 1253
HindIII AAGCTT 3 cut(s) 1892, 1950, 2499
HinfI GANTC 5 cut(s) 829, 1456, 1516, 1530, 1855
HpaII CCGG 4 cut(s) 616, 694, 1067, 2515
HphI GGTGA 2 cut(s) 972, 1004
Hpy166II GTNNAC 1 cut(s) 1991
Hpy188III TCNNGA 8 cut(s) 85, 108, 1128, 1326, 1422, 1500, 1793, 2003
Hpy8I GTNNAC 1 cut(s) 1991
Hpy99I CGWCG 5 cut(s) 26, 29, 32, 71, 86
HpyAV CCTTC 6 cut(s) 26, 82, 296, 957, 1706, 1818
HpyCH4III ACNGT 6 cut(s) 148, 534, 871, 892, 1560, 2475
HpyCH4IV ACGT 3 cut(s) 81, 1063, 1236
HpySE526I ACGT 3 cut(s) 81, 1063, 1236
HspAI GCGC 2 cut(s) 607, 1253
Kzo9I GATC 5 cut(s) 18, 182, 625, 818, 1795
LmnI GCTCC 4 cut(s) 457, 769, 880, 2412
MaeI CTAG 4 cut(s) 266, 402, 1704, 1838
MaeII ACGT 3 cut(s) 81, 1063, 1236
MaeIII GTNAC 8 cut(s) 163, 404, 778, 1048, 1361, 1560, 1599, 2517
MalI GATC 5 cut(s) 20, 184, 627, 820, 1797
MboI GATC 5 cut(s) 18, 182, 625, 818, 1795
MboII GAAGA 7 cut(s) 132, 229, 635, 969, 978, 1206, 1239
MfeI CAATTG 3 cut(s) 294, 1910, 2240
MflI RGATCY 3 cut(s) 182, 625, 818
MhlI GDGCHC 3 cut(s) 539, 1286, 1621
MlsI TGGCCA 3 cut(s) 1175, 1476, 1759
MluNI TGGCCA 3 cut(s) 1175, 1476, 1759
MmeI TCCRAC 4 cut(s) 197, 818, 829, 986
Mox20I TGGCCA 3 cut(s) 1175, 1476, 1759
Mph1103I ATGCAT 1 cut(s) 1182
MroXI GAANNNNTTC 1 cut(s) 1726
MscI TGGCCA 3 cut(s) 1175, 1476, 1759
MseI TTAA 6 cut(s) 119, 320, 704, 1293, 2019, 2587
MslI CAYNNNNRTG 1 cut(s) 1451
Msp20I TGGCCA 3 cut(s) 1175, 1476, 1759
MspA1I CMGCKG 3 cut(s) 16, 2116, 2398
MspI CCGG 4 cut(s) 616, 694, 1067, 2515
MspR9I CCNGG 9 cut(s) 616, 617, 694, 695, 714, 1401, 1527, 1980, 2571
MunI CAATTG 3 cut(s) 294, 1910, 2240
Mva1269I GAATGC 1 cut(s) 387
MvaI CCWGG 5 cut(s) 714, 1401, 1527, 1980, 2571
NciI CCSGG 4 cut(s) 616, 617, 694, 695
NcoI CCATGG 2 cut(s) 45, 1477
NdeII GATC 5 cut(s) 18, 182, 625, 818, 1795
NlaIV GGNNCC 4 cut(s) 51, 882, 1275, 1426
NmuCI GTSAC 5 cut(s) 163, 1048, 1560, 1599, 2517
NsiI ATGCAT 1 cut(s) 1182
NspI RCATGY 3 cut(s) 929, 1184, 1348
PaqCI CACCTGC 1 cut(s) 260
PasI CCCWGGG 1 cut(s) 1979
PciI ACATGT 1 cut(s) 925
PcsI WCGNNNNNNNCGW 1 cut(s) 27
PctI GAATGC 1 cut(s) 387
PdmI GAANNNNTTC 1 cut(s) 1726
PfeI GAWTC 5 cut(s) 829, 1456, 1516, 1530, 1855
PflMI CCANNNNNTGG 2 cut(s) 989, 1156
PfoI TCCNGGA 2 cut(s) 712, 1525
PpuMI RGGWCCY 1 cut(s) 1424
PscI ACATGT 1 cut(s) 925
Psp124BI GAGCTC 1 cut(s) 1286
Psp5II RGGWCCY 1 cut(s) 1424
Psp6I CCWGG 5 cut(s) 712, 1399, 1525, 1978, 2569
PspGI CCWGG 5 cut(s) 712, 1399, 1525, 1978, 2569
PspN4I GGNNCC 4 cut(s) 51, 882, 1275, 1426
PspPI GGNCC 9 cut(s) 50, 60, 691, 785, 911, 1424, 1543, 1827, 2558
PspPPI RGGWCCY 1 cut(s) 1424
PsrI GAACNNNNNNTAC 2 cut(s) 225, 257
PstI CTGCAG 4 cut(s) 1120, 2121, 2283, 2439
PstNI CAGNNNCTG 3 cut(s) 1223, 1736, 2398
PsuI RGATCY 3 cut(s) 182, 625, 818
PvuII CAGCTG 3 cut(s) 16, 2116, 2398
RsaI GTAC 3 cut(s) 204, 721, 1510
RsaNI GTAC 3 cut(s) 203, 720, 1509
RseI CAYNNNNRTG 1 cut(s) 1451
SacI GAGCTC 1 cut(s) 1286
SaqAI TTAA 6 cut(s) 119, 320, 704, 1293, 2019, 2587
Sau3AI GATC 5 cut(s) 18, 182, 625, 818, 1795
Sau96I GGNCC 9 cut(s) 50, 60, 691, 785, 911, 1424, 1543, 1827, 2558
ScrFI CCNGG 9 cut(s) 616, 617, 694, 695, 714, 1401, 1527, 1980, 2571
SduI GDGCHC 3 cut(s) 539, 1286, 1621
SfiI GGCCNNNNNGGCC 1 cut(s) 58
SinI GGWCC 2 cut(s) 911, 1424
SmaI CCCGGG 2 cut(s) 617, 695
SmiMI CAYNNNNRTG 1 cut(s) 1451
SmlI CTYRAG 1 cut(s) 1095
SmoI CTYRAG 1 cut(s) 1095
SrfI GCCCGGGC 1 cut(s) 695
SspMI CTAG 4 cut(s) 266, 402, 1704, 1838
SstI GAGCTC 1 cut(s) 1286
StyD4I CCNGG 9 cut(s) 614, 615, 692, 693, 712, 1399, 1525, 1978, 2569
StyI CCWWGG 5 cut(s) 45, 952, 1339, 1369, 1477
TaaI ACNGT 6 cut(s) 148, 534, 871, 892, 1560, 2475
TaiI ACGT 3 cut(s) 84, 1066, 1239
TaqI TCGA 3 cut(s) 30, 84, 1199
TatI WGTACW 1 cut(s) 719
TauI GCSGC 5 cut(s) 44, 1959, 2104, 2164, 2194
TfiI GAWTC 5 cut(s) 829, 1456, 1516, 1530, 1855
Tru1I TTAA 6 cut(s) 119, 320, 704, 1293, 2019, 2587
Tru9I TTAA 6 cut(s) 119, 320, 704, 1293, 2019, 2587
TscAI CASTG 3 cut(s) 539, 895, 1224
TseFI GTSAC 5 cut(s) 163, 1048, 1560, 1599, 2517
Tsp45I GTSAC 5 cut(s) 163, 1048, 1560, 1599, 2517
TspDTI ATGAA 9 cut(s) 842, 1013, 1124, 1233, 1421, 1469, 1817, 1868, 2535
TspGWI ACGGA 2 cut(s) 223, 1584
TspMI CCCGGG 2 cut(s) 615, 693
TspRI CASTG 3 cut(s) 539, 895, 1224
Van91I CCANNNNNTGG 2 cut(s) 989, 1156
VpaK11BI GGWCC 2 cut(s) 911, 1424
XagI CCTNNNNNAGG 2 cut(s) 267, 951
XapI RAATTY 6 cut(s) 469, 679, 1111, 1330, 1663, 2424
XceI RCATGY 3 cut(s) 929, 1184, 1348
XmaI CCCGGG 2 cut(s) 615, 693
XmiI GTMKAC 1 cut(s) 1990
XmnI GAANNNNTTC 1 cut(s) 1726
XspI CTAG 4 cut(s) 266, 402, 1704, 1838
Zsp2I ATGCAT 1 cut(s) 1182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.