Rh4DG345600

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0005569
Physical Location & Seq
Reverse (-)
56711889 .. 56723808
11920 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG345600.1

Sequence Viewer

Length: 2367 bp
ATGTCTTTGGTCACATTCAATACTCATGGATCTTATTCCGGTTGCTTGGTACAACGGAGTGGTTGGACAAGAGATGTTGATCGTTTCTTACAATGGCTGTCAGCTATTCCCTTAGCAGGTGGTGGTTTTAGTGATGCTGCAATTGCAGAAGGGCTTTCTGAAGCCCTAATGATGTTCACTAACATTCAAAATGGAAGCCAAAATCAACAAAATGTGGATTTTCAAAAGCATTGCATTCTTGTTGCCGCTAGTAACCCTCATCCCCTGCCCACTCCAGTCTATAGACCACAAATTCAAAATGTGGAGAAGAGTGAAAATATTGATGCACAAACCGAAAGCCGTCTGTATGATGCTGAGGCTGTTGCTAAATCATTTGCACAGTGCCTTGTTTCTCTGTCAGTCATCTGTCCGAAACAGCTTCCTAAACTCAGAGCCATTTACAATGCGGGAAAGCGCAACCCCCGGGCAGCAGATCCACCGATTGATAATGTGAAAAACCCCCACTTTCTCGTTCTAATCTCAGAGAATTTTATGGAGGCGCGGGCTACTTTAAGTCGTCCCGGAAGTACAACTTTGCCCTCAAATCCGAGTACTGTAAAGATGGACATAGCTCCTGTTGCATCAGTGACAGGGCCACCTCCAACTTCTATGCCATCTGTGAATGGATCTGTTATGAATCGGCCATCAGTTTCTGTTAACAATGTGCCTCCTGCGACAGTGAAAGTGGAGCCAAGCACTGTAAGTTCAATGGTAGCTGGACCTCCTTTTGCACACGTTCCATCTGTTCGTCCTCCTTCCCAAGGAGTTCCAACCTTGCAAACTTCTTCACCATCTTCTGGTGAGATGATTACAAACAATGAGAGTGTGCCAGATTTGAAACCTATAGTGAGTAATGTGACACATCAACTACGTCCTGTGGGTAGCATCCTCAATAATATCTCTCAAGCACGCGTGATGAATTCTGCAGCCTTATCTGGAGGATCTTCTATAGGTCTCCAAAACATGGGTCAAAATCCTATGGCCATGCATGTATCAAACATGATATCGAGTGGAATGGCATCTTCGGTGGCTGCTACTCAAAATGTATTTTCATCTTCAGGGCCTCACACTCTTGCACAGGTTGCTCAGAGCACAGGTCTTAGTTCATTTACTTCAGGTACTTCCAATGTGTCTGGAAATTCTAACCTTGGGATGTCGCAACCAATGAGTAACCTCCAAGGTGGTGTTAGCATTGGCCAATCTGTTCCTGGCATGAGTCAAGGAAATCTCTCAGGACCCCAGATGGTTCAAAGTGGCATTGGAATGACTCCAAACATGATGAGTGGCCTTGGTTCATCAGGTACCTCCTCTGGAACTGGGACAATGATTCCCACTCCAGGCATGTCTCAACAGGCACAATCTGGGATGCAGTCACTTGGTGCAAACAATAGTTCAGTTTCTAATGTGCCGTTGTCACAACAGACGTCAAGTGCTCTGCAGTCTGCACAATCCAAATATGTGAAAGTGTGGGAGGGAAACTTATCTGGGCAGAGACAAGGGCAACCTGTGTTTATTACTAGATTGGAGGGATACAGAAGTGCTACAGCATCTGAGACGCTTGCAGCAAACTGGCCACCGACAATGCAAATAGTCAGGCTCATATCTCAGGATCACATGAATAACAAGCAATATGTGGGAAGGGCAGACTTTCTAGTATTTCGGGCAATGAATCAGCATGGATTTCTTGGGCAGTTGCAAGAAAAGAAGCTTTGTGCTGTCATCCAATTGCCATCTCAGACACTACTGCTCTCTGTTTCGGACAAAGCTTGCCGCTTGATTGGAATGCTTTTCCCAGGGGATATGGTTGTGTTTAAGCCACAAGTAGGCGGTCAGCAGCAGCAGATGCAACAAATGCAACAACAGCAGCCGCAGCTACAACAGATGCAGCAACAGCAACCACAGCTCCAGCAACAACAGCAACCGCAACTGCAGCAGCAGCAGCATCCACAAATGCAACAACAGCAACAGCAACCGCAACTGCAGCAGCAGCAGCAGCAGCATCCGCAGATGCAACAACAGCAGCAGCATCCACAGATGCAACAGCAGCAGCAGCAGCAGCATCCACAGATGCAACAGCAGCAGCAGCAGCTTCCGCAGATGCAGCAGCAACAGCAGCATCCACAGATGCAACAGCAGCAGCTCTCGCAGCTCCAGTCACAACAGCAAATTCCACAACTGCAGCAACTGCAGCAACAACACCAACAGCAACAGTTGGTTGGGGGTGGAATGGGTCAAGCTTATGGTCAAGGCCGGTCACAATTACTTTCACAAGGACAAGTTTCATCACAACAAGGGCCTAATATGCCCGGAGGAGGCTTTATGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med25_VWA

Protein Analysis

788

Amino Acids

85.04

Weight (kDa)

9.37

Isoelectric Point (pI)

65.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med25_VWA PF11265 1 - 175 1.1e-33 Mediator complex subunit 25 von Willebrand factor type A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 107
AatII GACGTC 1 cut(s) 1466
Acc36I ACCTGC 1 cut(s) 107
Acc65I GGTACC 1 cut(s) 1340
AccB1I GGYRCC 1 cut(s) 1340
AccB7I CCANNNNNTGG 2 cut(s) 836, 1003
AccII CGCG 2 cut(s) 541, 951
AclWI GGATC 5 cut(s) 37, 467, 673, 988, 1656
AcoI YGGCCR 4 cut(s) 680, 1020, 1234, 1610
AcsI RAATTY 5 cut(s) 291, 526, 958, 1177, 2205
AcuI CTGAAG 3 cut(s) 180, 1080, 1137
AcyI GRCGYC 1 cut(s) 1463
AdeI CACNNNGTG 1 cut(s) 1418
AfaI GTAC 5 cut(s) 51, 568, 592, 1159, 1342
AfiI CCNNNNNNNGG 7 cut(s) 116, 800, 836, 1003, 1376, 1862, 2351
AflIII ACRYGT 2 cut(s) 772, 949
AgsI TTSAA 7 cut(s) 19, 188, 224, 296, 747, 877, 1289
AjnI CCWGG 3 cut(s) 1246, 1375, 1831
Alw21I GWGCWC 2 cut(s) 1133, 1474
Alw26I GTCTC 4 cut(s) 998, 1389, 1525, 1586
AlwI GGATC 5 cut(s) 37, 467, 673, 988, 1656
AlwNI CAGNNNCTG 3 cut(s) 692, 1589, 2224
Ama87I CYCGRG 1 cut(s) 462
AoxI GGCC 9 cut(s) 632, 680, 1020, 1100, 1234, 1324, 1610, 2287, 2333
ApoI RAATTY 5 cut(s) 291, 526, 958, 1177, 2205
ArsI GACNNNNNNTTYG 2 cut(s) 991, 1023
Asp718I GGTACC 1 cut(s) 1340
AspLEI GCGC 2 cut(s) 456, 541
AspS9I GGNCC 5 cut(s) 632, 758, 1100, 1274, 2333
AsuC2I CCSGG 4 cut(s) 463, 464, 561, 2346
AsuHPI GGTGA 2 cut(s) 819, 851
AvaI CYCGRG 1 cut(s) 462
AvaII GGWCC 2 cut(s) 758, 1274
BaeI ACNNNNGTAYC 2 cut(s) 1561, 1594
BalI TGGCCA 3 cut(s) 1022, 1236, 1612
BanI GGYRCC 1 cut(s) 1340
Bbv12I GWGCWC 2 cut(s) 1133, 1474
BbvCI CCTCAGC 1 cut(s) 354
BccI CCATC 7 cut(s) 595, 661, 691, 787, 838, 1276, 1777
BceAI ACGGC 2 cut(s) 324, 1432
BciT130I CCWGG 3 cut(s) 1248, 1377, 1833
BciVI GTATCC 1 cut(s) 1562
BcnI CCSGG 4 cut(s) 463, 464, 561, 2346
BcoDI GTCTC 4 cut(s) 998, 1389, 1525, 1586
BfaI CTAG 3 cut(s) 249, 1557, 1691
BfuAI ACCTGC 1 cut(s) 107
BfuI GTATCC 1 cut(s) 1562
BmcAI AGTACT 1 cut(s) 592
Bme1390I CCNGG 7 cut(s) 463, 464, 561, 1248, 1377, 1833, 2346
Bme18I GGWCC 2 cut(s) 758, 1274
BmeT110I CYCGRG 1 cut(s) 462
BmgT120I GGNCC 5 cut(s) 632, 758, 1100, 1274, 2333
BmiI GGNNCC 3 cut(s) 729, 1276, 1342
BmrFI CCNGG 7 cut(s) 463, 464, 561, 1248, 1377, 1833, 2346
BmrI ACTGGG 1 cut(s) 1365
BmuI ACTGGG 1 cut(s) 1365
BpmI CTGGAG 5 cut(s) 258, 996, 1359, 1928, 2174
Bpu10I CCTNAGC 2 cut(s) 112, 354
BpuEI CTTGAG 1 cut(s) 927
BpuMI CCSGG 4 cut(s) 463, 464, 561, 2346
BsaBI GATNNNNATC 1 cut(s) 78
BsaHI GRCGYC 1 cut(s) 1463
BsaI GGTCTC 1 cut(s) 998
BsaJI CCNNGG 8 cut(s) 461, 462, 799, 1186, 1216, 1327, 1831, 1832
BsaWI WCCGGW 1 cut(s) 38
BsaXI ACNNNNNCTCC 2 cut(s) 1926, 1956
Bsc4I CCNNNNNNNGG 7 cut(s) 116, 800, 836, 1003, 1376, 1862, 2351
Bse118I RCCGGY 1 cut(s) 2289
Bse1I ACTGG 4 cut(s) 275, 1360, 1613, 2191
Bse3DI GCAATG 2 cut(s) 229, 1710
Bse8I GATNNNNATC 1 cut(s) 78
BseBI CCWGG 3 cut(s) 1248, 1377, 1833
BseDI CCNNGG 8 cut(s) 461, 462, 799, 1186, 1216, 1327, 1831, 1832
BseJI GATNNNNATC 1 cut(s) 78
BseLI CCNNNNNNNGG 7 cut(s) 116, 800, 836, 1003, 1376, 1862, 2351
BseMI GCAATG 2 cut(s) 229, 1710
BseMII CTCAG 8 cut(s) 345, 442, 534, 1139, 1284, 1581, 1658, 1787
BseNI ACTGG 4 cut(s) 275, 1360, 1613, 2191
BseRI GAGGAG 2 cut(s) 1336, 2364
BsgI GTGCAG 1 cut(s) 1467
Bsh1236I CGCG 2 cut(s) 541, 951
BshFI GGCC 9 cut(s) 634, 682, 1022, 1102, 1236, 1326, 1612, 2289, 2335
BshNI GGYRCC 1 cut(s) 1340
BsiHKAI GWGCWC 2 cut(s) 1133, 1474
BsiHKCI CYCGRG 1 cut(s) 462
BsiSI CCGG 5 cut(s) 39, 463, 561, 2290, 2346
BslFI GGGAC 2 cut(s) 543, 1372
BslI CCNNNNNNNGG 7 cut(s) 116, 800, 836, 1003, 1376, 1862, 2351
BsmAI GTCTC 4 cut(s) 998, 1389, 1525, 1586
BsmBI CGTCTC 1 cut(s) 1586
BsmFI GGGAC 2 cut(s) 543, 1372
BsmI GAATGC 2 cut(s) 234, 1827
BsnI GGCC 9 cut(s) 634, 682, 1022, 1102, 1236, 1326, 1612, 2289, 2335
Bso31I GGTCTC 1 cut(s) 998
BsoBI CYCGRG 1 cut(s) 462
Bsp1286I GDGCHC 2 cut(s) 1133, 1474
Bsp143I GATC 6 cut(s) 29, 79, 472, 665, 980, 1648
BspANI GGCC 9 cut(s) 634, 682, 1022, 1102, 1236, 1326, 1612, 2289, 2335
BspCNI CTCAG 8 cut(s) 346, 441, 533, 1138, 1283, 1582, 1657, 1786
BspFNI CGCG 2 cut(s) 541, 951
BspLI GGNNCC 3 cut(s) 729, 1276, 1342
BspMAI CTGCAG 6 cut(s) 967, 1479, 1971, 2022, 2220, 2229
BspMI ACCTGC 1 cut(s) 107
BspPI GGATC 5 cut(s) 37, 467, 673, 988, 1656
BspT107I GGYRCC 1 cut(s) 1340
BspTNI GGTCTC 1 cut(s) 998
BsrDI GCAATG 2 cut(s) 229, 1710
BsrFI RCCGGY 1 cut(s) 2289
BsrI ACTGG 4 cut(s) 275, 1360, 1613, 2191
BssAI RCCGGY 1 cut(s) 2289
BssECI CCNNGG 8 cut(s) 461, 462, 799, 1186, 1216, 1327, 1831, 1832
BssMI GATC 6 cut(s) 29, 79, 472, 665, 980, 1648
BssNI GRCGYC 1 cut(s) 1463
BssT1I CCWWGG 4 cut(s) 799, 1186, 1216, 1327
Bst2UI CCWGG 3 cut(s) 1248, 1377, 1833
Bst4CI ACNGT 5 cut(s) 381, 595, 718, 739, 2250
Bst6I CTCTTC 1 cut(s) 302
BstACI GRCGYC 1 cut(s) 1463
BstAPI GCANNNNNTGC 4 cut(s) 1121, 1882, 1891, 2224
BstC8I GCNNGC 4 cut(s) 543, 949, 1599, 1807
BstENI CCTNNNNNAGG 1 cut(s) 798
BstFNI CGCG 2 cut(s) 541, 951
BstHHI GCGC 2 cut(s) 456, 541
BstKTI GATC 6 cut(s) 32, 82, 475, 668, 983, 1651
BstMAI GTCTC 4 cut(s) 998, 1389, 1525, 1586
BstMBI GATC 6 cut(s) 29, 79, 472, 665, 980, 1648
BstNI CCWGG 3 cut(s) 1248, 1377, 1833
BstNSI RCATGY 2 cut(s) 1031, 1384
BstSCI CCNGG 7 cut(s) 461, 462, 559, 1246, 1375, 1831, 2344
BstUI CGCG 2 cut(s) 541, 951
BstX2I RGATCY 4 cut(s) 29, 472, 665, 980
BstYI RGATCY 4 cut(s) 29, 472, 665, 980
BsuI GTATCC 1 cut(s) 1562
BsuRI GGCC 9 cut(s) 634, 682, 1022, 1102, 1236, 1326, 1612, 2289, 2335
BtsIMutI CAGTG 4 cut(s) 386, 630, 723, 735
BveI ACCTGC 1 cut(s) 107
Cac8I GCNNGC 4 cut(s) 543, 949, 1599, 1807
CaiI CAGNNNCTG 3 cut(s) 692, 1589, 2224
CfoI GCGC 2 cut(s) 456, 541
Cfr10I RCCGGY 1 cut(s) 2289
Cfr13I GGNCC 5 cut(s) 632, 758, 1100, 1274, 2333
Cfr9I CCCGGG 1 cut(s) 462
CseI GACGC 1 cut(s) 1603
Csp6I GTAC 5 cut(s) 50, 567, 591, 1158, 1341
CviQI GTAC 5 cut(s) 50, 567, 591, 1158, 1341
DpnI GATC 6 cut(s) 31, 81, 474, 667, 982, 1650
DpnII GATC 6 cut(s) 29, 79, 472, 665, 980, 1648
DraIII CACNNNGTG 1 cut(s) 1418
EaeI YGGCCR 4 cut(s) 680, 1020, 1234, 1610
Eam1104I CTCTTC 1 cut(s) 302
EarI CTCTTC 1 cut(s) 302
Eco130I CCWWGG 4 cut(s) 799, 1186, 1216, 1327
Eco31I GGTCTC 1 cut(s) 998
Eco32I GATATC 1 cut(s) 1044
Eco47I GGWCC 2 cut(s) 758, 1274
Eco57I CTGAAG 3 cut(s) 180, 1080, 1137
Eco88I CYCGRG 1 cut(s) 462
EcoNI CCTNNNNNAGG 1 cut(s) 798
EcoO109I RGGNCCY 3 cut(s) 1100, 1274, 2333
EcoRI GAATTC 1 cut(s) 958
EcoRII CCWGG 3 cut(s) 1246, 1375, 1831
EcoRV GATATC 1 cut(s) 1044
EcoT14I CCWWGG 4 cut(s) 799, 1186, 1216, 1327
EcoT22I ATGCAT 1 cut(s) 1029
ErhI CCWWGG 4 cut(s) 799, 1186, 1216, 1327
Esp3I CGTCTC 1 cut(s) 1586
FalI AAGNNNNNCTT 2 cut(s) 556, 588
FaqI GGGAC 2 cut(s) 543, 1372
FauI CCCGC 2 cut(s) 439, 534
FspBI CTAG 3 cut(s) 249, 1557, 1691
GlaI GCGC 2 cut(s) 455, 540
GsuI CTGGAG 5 cut(s) 258, 996, 1359, 1928, 2174
HaeIII GGCC 9 cut(s) 634, 682, 1022, 1102, 1236, 1326, 1612, 2289, 2335
HapII CCGG 5 cut(s) 39, 463, 561, 2290, 2346
HgaI GACGC 1 cut(s) 1603
HhaI GCGC 2 cut(s) 456, 541
Hin1I GRCGYC 1 cut(s) 1463
Hin6I GCGC 2 cut(s) 454, 539
HinP1I GCGC 2 cut(s) 454, 539
HincII GTYRAC 1 cut(s) 697
HindII GTYRAC 1 cut(s) 697
HindIII AAGCTT 3 cut(s) 1745, 1803, 2274
HinfI GANTC 5 cut(s) 676, 1255, 1306, 1366, 1708
HpaI GTTAAC 1 cut(s) 697
HpaII CCGG 5 cut(s) 39, 463, 561, 2290, 2346
HphI GGTGA 2 cut(s) 819, 851
Hpy166II GTNNAC 2 cut(s) 177, 697
Hpy188I TCNGA 9 cut(s) 160, 411, 431, 523, 588, 1128, 1591, 1776, 1798
Hpy188III TCNNGA 5 cut(s) 975, 1173, 1272, 1350, 1646
Hpy8I GTNNAC 2 cut(s) 177, 697
HpyAV CCTTC 3 cut(s) 143, 804, 1671
HpyCH4III ACNGT 5 cut(s) 381, 595, 718, 739, 2250
HpyCH4IV ACGT 3 cut(s) 774, 910, 1463
HpySE526I ACGT 3 cut(s) 774, 910, 1463
Hsp92I GRCGYC 1 cut(s) 1463
HspAI GCGC 2 cut(s) 454, 539
KpnI GGTACC 1 cut(s) 1344
KspAI GTTAAC 1 cut(s) 697
Kzo9I GATC 6 cut(s) 29, 79, 472, 665, 980, 1648
LmnI GCTCC 4 cut(s) 616, 727, 1947, 2193
MaeI CTAG 3 cut(s) 249, 1557, 1691
MaeII ACGT 3 cut(s) 774, 910, 1463
MaeIII GTNAC 9 cut(s) 10, 251, 625, 895, 1208, 1410, 1452, 2193, 2292
MalI GATC 6 cut(s) 31, 81, 474, 667, 982, 1650
MboI GATC 6 cut(s) 29, 79, 472, 665, 980, 1648
MboII GAAGA 6 cut(s) 319, 816, 825, 975, 1053, 1086
MfeI CAATTG 2 cut(s) 141, 1763
MflI RGATCY 4 cut(s) 29, 472, 665, 980
MhlI GDGCHC 2 cut(s) 1133, 1474
MlsI TGGCCA 3 cut(s) 1022, 1236, 1612
MluCI AATT 8 cut(s) 141, 291, 526, 958, 1177, 1763, 2205, 2297
MluI ACGCGT 1 cut(s) 949
MluNI TGGCCA 3 cut(s) 1022, 1236, 1612
MlyI GAGTC 2 cut(s) 1264, 1300
MmeI TCCRAC 3 cut(s) 44, 665, 833
Mox20I TGGCCA 3 cut(s) 1022, 1236, 1612
Mph1103I ATGCAT 1 cut(s) 1029
MscI TGGCCA 3 cut(s) 1022, 1236, 1612
MseI TTAA 4 cut(s) 551, 696, 1851, 2365
MslI CAYNNNNRTG 1 cut(s) 1301
Msp20I TGGCCA 3 cut(s) 1022, 1236, 1612
MspI CCGG 5 cut(s) 39, 463, 561, 2290, 2346
MspR9I CCNGG 7 cut(s) 463, 464, 561, 1248, 1377, 1833, 2346
MunI CAATTG 2 cut(s) 141, 1763
Mva1269I GAATGC 2 cut(s) 234, 1827
MvaI CCWGG 3 cut(s) 1248, 1377, 1833
MvnI CGCG 2 cut(s) 541, 951
NciI CCSGG 4 cut(s) 463, 464, 561, 2346
NdeII GATC 6 cut(s) 29, 79, 472, 665, 980, 1648
NlaIV GGNNCC 3 cut(s) 729, 1276, 1342
NmuCI GTSAC 7 cut(s) 10, 625, 895, 1410, 1452, 2193, 2292
NsiI ATGCAT 1 cut(s) 1029
NspI RCATGY 2 cut(s) 1031, 1384
PaqCI CACCTGC 1 cut(s) 107
PasI CCCWGGG 1 cut(s) 1832
PctI GAATGC 2 cut(s) 234, 1827
PfeI GAWTC 3 cut(s) 676, 1366, 1708
PflMI CCANNNNNTGG 2 cut(s) 836, 1003
PfoI TCCNGGA 1 cut(s) 559
PleI GAGTC 2 cut(s) 1263, 1300
PpsI GAGTC 2 cut(s) 1263, 1300
PpuMI RGGWCCY 1 cut(s) 1274
Psp5II RGGWCCY 1 cut(s) 1274
Psp6I CCWGG 3 cut(s) 1246, 1375, 1831
PspGI CCWGG 3 cut(s) 1246, 1375, 1831
PspN4I GGNNCC 3 cut(s) 729, 1276, 1342
PspPI GGNCC 5 cut(s) 632, 758, 1100, 1274, 2333
PspPPI RGGWCCY 1 cut(s) 1274
PstI CTGCAG 6 cut(s) 967, 1479, 1971, 2022, 2220, 2229
PstNI CAGNNNCTG 3 cut(s) 692, 1589, 2224
PsuI RGATCY 4 cut(s) 29, 472, 665, 980
RsaI GTAC 5 cut(s) 51, 568, 592, 1159, 1342
RsaNI GTAC 5 cut(s) 50, 567, 591, 1158, 1341
RseI CAYNNNNRTG 1 cut(s) 1301
SaqAI TTAA 4 cut(s) 551, 696, 1851, 2365
Sau3AI GATC 6 cut(s) 29, 79, 472, 665, 980, 1648
Sau96I GGNCC 5 cut(s) 632, 758, 1100, 1274, 2333
ScaI AGTACT 1 cut(s) 592
SchI GAGTC 2 cut(s) 1264, 1300
ScrFI CCNGG 7 cut(s) 463, 464, 561, 1248, 1377, 1833, 2346
SduI GDGCHC 2 cut(s) 1133, 1474
SinI GGWCC 2 cut(s) 758, 1274
SmaI CCCGGG 1 cut(s) 464
SmiMI CAYNNNNRTG 1 cut(s) 1301
SmlI CTYRAG 1 cut(s) 942
SmoI CTYRAG 1 cut(s) 942
Sse9I AATT 8 cut(s) 141, 291, 526, 958, 1177, 1763, 2205, 2297
SspI AATATT 1 cut(s) 319
SspMI CTAG 3 cut(s) 249, 1557, 1691
StyD4I CCNGG 7 cut(s) 461, 462, 559, 1246, 1375, 1831, 2344
StyI CCWWGG 4 cut(s) 799, 1186, 1216, 1327
TaaI ACNGT 5 cut(s) 381, 595, 718, 739, 2250
TaiI ACGT 3 cut(s) 777, 913, 1466
TaqI TCGA 1 cut(s) 1046
TasI AATT 8 cut(s) 141, 291, 526, 958, 1177, 1763, 2205, 2297
TatI WGTACW 2 cut(s) 566, 590
TauI GCSGC 3 cut(s) 248, 1812, 1909
TfiI GAWTC 3 cut(s) 676, 1366, 1708
Tru1I TTAA 4 cut(s) 551, 696, 1851, 2365
Tru9I TTAA 4 cut(s) 551, 696, 1851, 2365
TscAI CASTG 4 cut(s) 386, 630, 723, 742
TseFI GTSAC 7 cut(s) 10, 625, 895, 1410, 1452, 2193, 2292
Tsp45I GTSAC 7 cut(s) 10, 625, 895, 1410, 1452, 2193, 2292
TspDTI ATGAA 8 cut(s) 689, 971, 1080, 1134, 1323, 1670, 1721, 2310
TspGWI ACGGA 1 cut(s) 70
TspMI CCCGGG 1 cut(s) 462
TspRI CASTG 4 cut(s) 386, 630, 723, 742
Van91I CCANNNNNTGG 2 cut(s) 836, 1003
VpaK11BI GGWCC 2 cut(s) 758, 1274
XagI CCTNNNNNAGG 1 cut(s) 798
XapI RAATTY 5 cut(s) 291, 526, 958, 1177, 2205
XceI RCATGY 2 cut(s) 1031, 1384
XcmI CCANNNNNNNNNTGG 1 cut(s) 1244
XmaI CCCGGG 1 cut(s) 462
XspI CTAG 3 cut(s) 249, 1557, 1691
ZraI GACGTC 1 cut(s) 1464
ZrmI AGTACT 1 cut(s) 592
Zsp2I ATGCAT 1 cut(s) 1029
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.