Rh4BG351300

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0005569
Physical Location & Seq
Reverse (-)
51225138 .. 51235238
10101 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG351300.1

Sequence Viewer

Length: 2499 bp
ATGACGGAGAAGCAGTTGATCGTGGTCGTTGAAGGCACGGCGGCCATCGGGCCTCACTGGTCAGCCGTCGTCTCCGATTACGTCGAGAAGATCATCAGGTCTTTCTCTGGAAACGAGTTAAGTGGTCAGAAGCCCTCCACTTCTATTGTTGAGATGTCTTTGGTCACATTCAATACTCATGGATCTTATTCCGGTTGCTTGGTACAACGGAGTGGTTGGACAAGAGATGTTGATCGTTTCTTACAATGGCTGTCAGCTATTCCCTTAGCAGGTGGTGGTTTTAGTGATGCTGCAATTGCAGAAGGGCTTTCTGAAGCCCTAATGATGTTCCCTACCGTTCAAAATGGAAGCCAAAATCAACAAAATGTGGATTTTCAAAAGCATTGCATTCTTGTTGCTGCTAGTAACCCTCATCCCCTGCCCACTCCAGTCTATAGGCCACAAATTCAAAATGTGGAGAAGAGTGAAAATATTGATGCACAAACCGAAAGCCGTCTGTATGATGCTGAGGCTGTTGCTAAATCATTTGCACAGTGCCTTGTTTCTCTGTCAGTCATCTGTCCGAAACAGCTTCCTAAACTCAGAGCCATTTACAATGCGGGAAAGCGCAACCCCCGGGCAGCAGATCCACCGATTGATAATGTGAAAAACCCCCACTTTCTCGTTCTAATCTCAGAGAATTTTATGGAGGCCCGGGCTACTTTAAGTCGTTCCGGAAGTACAACTTTGCCCTCAAATCCGAGTACTGTAAAGATGGACATAGCTCCTGTTGCATCAGTGACAGGGCCGCCTCCAACTTCTATGCCATCCGTGAATGGATCTGTTATGAATCGGCCATCAGTTTCTGTTAACAATGTGCCTCCTGCGACAGTGAAAGTGGAGCCAACCACTGTAAGTTCAATGGTAGCTGGACCTGCTTTTGCACATGTTCGTCCTCCTTCCCAAGGAGTTCCAACCTTGCAAACTTCTTCACCATCTTCTGGTGAGATGATTACAAACAATGAGAGTGTGCCAGATTTGAAACCTATAGTGAGTAATGTGACACATCAACTACGTCCTGTGGGTAGCATCCTCAATAATATCTCTCAAGCACGCGTGATGAATTCTGCGGCCTTATCTGGAGGATCTTCTATAGGTCTCCAAAACATGGGTCAAAATCCTATGGCCATGCATGTATCAAACATGATATCGAGTGGAATGGCATCTTCGGTGGCTGCTGCTCAAAATGTATTTTCATCTTCAGGGCCTCACACTCTTGCACAGGTTGCTCAGAGCACAGGTCTTGGTTCATTTACTTCAGGTACTTCCAATGTGTCTGGAAATTCTAACCTTGGGATGTCGCAACCAATGAGTAACCTCCAAGGTGGTGTTAGCATTGGCCAATCTGTTCCTGGCATGAGTCAAGGAAATCTCTCAGGTCCCCAGATGGTTCAAAGTGGCATTGGAATGACTCCAAACATGATGAGTGGCCTTGGTTCATCAGGTACCTCCTCTGGAACTGGGACAATGATTCCCACTCCAGGCATGTCTCAACAGGCACAATCTGGGATGCAGTCACTTGGTGCAAACAATAGTTCAGTTTCTAATGTGCCGTTGTCACAACAGACGTCAAGTGCTCTGCAGTCTGCACAATCCAAATATGTGAAAGTGTGGGAGGGAAACTTATCTGGGCAGAGACAAGGGCAACCTGTGTTTATTACTAGATTGGAGGGATACAGAAGTGCTACAGCATCTGAGACGCTTGCAGCAAACTGGCCACCGACAATGCAAATAGTCAGGCTCATATCTCAGGATCACATGAATAACAAGCAATATGTGGGAAGGGCAGACTTTCTAGTATTTCGGGCAATGAATCAGCATGGATTTCTTGGGCAGTTGCAAGAAAAGAAGCTTTGTGCTGTCATCCAATTGCCGTCTCAGACACTACTGCTCTCTGTTTCGGACAAAGCTTGCCGCTTGATTGGAATGCTTTTCCCAGGGGATATGGTTGTGTTTAAGCCACAAGTAGGCGGTCAGCAGCAGCAGATGCAACAAATGCAACAACAGCAGCCGCAGCTACAACAGATGCAGCAACAGCAACCGCAGCTCCAGCAACAACCGCAACTGCAGCAGCAGCAGCATCCGCAAATGCAACAACAGCAACAGCAACCGCAACTGCAGCAGCAGCAGCATCCGCAGATGCAGCAACAGCAGCAGCATCCGCAGATGCAGCAACAGCAGCAGCATCCACAGATGCAACAGCAGCAGCAGCAGCAGCAGCTTCCGCAGATGCAACAACAGCAGCAACATCCTCAGATGCAACAGCAGCAGCTCTCGCAGCTCCAGTCACAACAGCAAATTCCACAACTGCAGCAACTGCAGCAACAACACCAACAGCAACAGTTGGTTGGGGGTGGAATGGGTCAAGCTTATGGTCAAGGCCGGTCACAATTACTTTCACAAGGACAAGTTTCATCACAACAAGGGCCTAATATGCCAGGAGGAGGCTTTATGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med25_VWA

Protein Analysis

832

Amino Acids

89.6

Weight (kDa)

9.14

Isoelectric Point (pI)

61.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med25_VWA PF11265 3 - 226 2.6e-43 Mediator complex subunit 25 von Willebrand factor type A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 260
AatII GACGTC 1 cut(s) 1610
Acc36I ACCTGC 2 cut(s) 260, 922
Acc65I GGTACC 1 cut(s) 1484
AccB1I GGYRCC 1 cut(s) 1484
AccB7I CCANNNNNTGG 2 cut(s) 980, 1147
AccII CGCG 1 cut(s) 1095
AccIII TCCGGA 1 cut(s) 713
AclWI GGATC 5 cut(s) 190, 620, 826, 1132, 1800
AcoI YGGCCR 5 cut(s) 42, 833, 1164, 1378, 1754
AcsI RAATTY 5 cut(s) 444, 679, 1102, 1321, 2337
AcuI CTGAAG 3 cut(s) 333, 1224, 1281
AcyI GRCGYC 1 cut(s) 1607
AdeI CACNNNGTG 1 cut(s) 1562
AfaI GTAC 5 cut(s) 204, 721, 745, 1303, 1486
AfiI CCNNNNNNNGG 7 cut(s) 269, 944, 980, 1147, 1520, 2006, 2483
AflIII ACRYGT 2 cut(s) 925, 1093
AgsI TTSAA 8 cut(s) 32, 172, 341, 377, 449, 900, 1021, 1433
AjnI CCWGG 4 cut(s) 1390, 1519, 1975, 2476
Alw21I GWGCWC 2 cut(s) 1277, 1618
Alw26I GTCTC 6 cut(s) 76, 1142, 1533, 1669, 1730, 1920
AlwI GGATC 5 cut(s) 190, 620, 826, 1132, 1800
AlwNI CAGNNNCTG 3 cut(s) 845, 1733, 2356
Ama87I CYCGRG 2 cut(s) 615, 693
Aor13HI TCCGGA 1 cut(s) 713
ApoI RAATTY 5 cut(s) 444, 679, 1102, 1321, 2337
ArsI GACNNNNNNTTYG 2 cut(s) 1135, 1167
Asp718I GGTACC 1 cut(s) 1484
AspLEI GCGC 1 cut(s) 609
AspS9I GGNCC 7 cut(s) 50, 691, 785, 911, 1244, 1418, 2465
AsuC2I CCSGG 4 cut(s) 616, 617, 694, 695
AsuHPI GGTGA 2 cut(s) 963, 995
AvaI CYCGRG 2 cut(s) 615, 693
AvaII GGWCC 2 cut(s) 911, 1418
BaeI ACNNNNGTAYC 2 cut(s) 1705, 1738
BalI TGGCCA 3 cut(s) 1166, 1380, 1756
BanI GGYRCC 1 cut(s) 1484
Bbv12I GWGCWC 2 cut(s) 1277, 1618
BbvCI CCTCAGC 1 cut(s) 507
BccI CCATC 6 cut(s) 53, 748, 814, 844, 982, 1420
BceAI ACGGC 5 cut(s) 50, 54, 477, 1576, 1897
BciT130I CCWGG 4 cut(s) 1392, 1521, 1977, 2478
BciVI GTATCC 1 cut(s) 1706
BcnI CCSGG 4 cut(s) 616, 617, 694, 695
BcoDI GTCTC 6 cut(s) 76, 1142, 1533, 1669, 1730, 1920
BfaI CTAG 3 cut(s) 402, 1701, 1835
BfmI CTRYAG 9 cut(s) 433, 1026, 1131, 1619, 1725, 2105, 2156, 2348, 2357
BfuAI ACCTGC 2 cut(s) 260, 922
BfuI GTATCC 1 cut(s) 1706
BmcAI AGTACT 1 cut(s) 745
Bme1390I CCNGG 8 cut(s) 616, 617, 694, 695, 1392, 1521, 1977, 2478
Bme18I GGWCC 2 cut(s) 911, 1418
BmeT110I CYCGRG 2 cut(s) 615, 693
BmgT120I GGNCC 7 cut(s) 50, 691, 785, 911, 1244, 1418, 2465
BmiI GGNNCC 3 cut(s) 882, 1420, 1486
BmrFI CCNGG 8 cut(s) 616, 617, 694, 695, 1392, 1521, 1977, 2478
BmrI ACTGGG 1 cut(s) 1509
BmuI ACTGGG 1 cut(s) 1509
BpmI CTGGAG 5 cut(s) 411, 1140, 1503, 2072, 2306
Bpu10I CCTNAGC 2 cut(s) 265, 507
BpuEI CTTGAG 1 cut(s) 1071
BpuMI CCSGG 4 cut(s) 616, 617, 694, 695
BsaBI GATNNNNATC 1 cut(s) 231
BsaHI GRCGYC 1 cut(s) 1607
BsaI GGTCTC 1 cut(s) 1142
BsaJI CCNNGG 9 cut(s) 614, 615, 693, 943, 1330, 1360, 1471, 1975, 1976
BsaWI WCCGGW 2 cut(s) 191, 713
BsaXI ACNNNNNCTCC 3 cut(s) 29, 2070, 2100
Bsc4I CCNNNNNNNGG 7 cut(s) 269, 944, 980, 1147, 1520, 2006, 2483
Bse118I RCCGGY 1 cut(s) 2421
Bse1I ACTGG 5 cut(s) 62, 428, 1504, 1757, 2323
Bse3DI GCAATG 2 cut(s) 382, 1854
Bse8I GATNNNNATC 1 cut(s) 231
BseAI TCCGGA 1 cut(s) 713
BseBI CCWGG 4 cut(s) 1392, 1521, 1977, 2478
BseDI CCNNGG 9 cut(s) 614, 615, 693, 943, 1330, 1360, 1471, 1975, 1976
BseJI GATNNNNATC 1 cut(s) 231
BseLI CCNNNNNNNGG 7 cut(s) 269, 944, 980, 1147, 1520, 2006, 2483
BseMI GCAATG 2 cut(s) 382, 1854
BseMII CTCAG 9 cut(s) 498, 595, 687, 1283, 1428, 1725, 1802, 1931, 2306
BseNI ACTGG 5 cut(s) 62, 428, 1504, 1757, 2323
BseRI GAGGAG 2 cut(s) 1480, 2496
BsgI GTGCAG 1 cut(s) 1611
Bsh1236I CGCG 1 cut(s) 1095
BshNI GGYRCC 1 cut(s) 1484
BsiHKAI GWGCWC 2 cut(s) 1277, 1618
BsiHKCI CYCGRG 2 cut(s) 615, 693
BsiSI CCGG 5 cut(s) 192, 616, 694, 714, 2422
BslFI GGGAC 2 cut(s) 1404, 1516
BslI CCNNNNNNNGG 7 cut(s) 269, 944, 980, 1147, 1520, 2006, 2483
BsmAI GTCTC 6 cut(s) 76, 1142, 1533, 1669, 1730, 1920
BsmBI CGTCTC 3 cut(s) 76, 1730, 1920
BsmFI GGGAC 2 cut(s) 1404, 1516
BsmI GAATGC 2 cut(s) 387, 1971
Bso31I GGTCTC 1 cut(s) 1142
BsoBI CYCGRG 2 cut(s) 615, 693
Bsp1286I GDGCHC 2 cut(s) 1277, 1618
Bsp13I TCCGGA 1 cut(s) 713
Bsp143I GATC 8 cut(s) 18, 90, 182, 232, 625, 818, 1124, 1792
BspCNI CTCAG 9 cut(s) 499, 594, 686, 1282, 1427, 1726, 1801, 1930, 2305
BspEI TCCGGA 1 cut(s) 713
BspFNI CGCG 1 cut(s) 1095
BspLI GGNNCC 3 cut(s) 882, 1420, 1486
BspMAI CTGCAG 5 cut(s) 1623, 2109, 2160, 2352, 2361
BspMI ACCTGC 2 cut(s) 260, 922
BspPI GGATC 5 cut(s) 190, 620, 826, 1132, 1800
BspT107I GGYRCC 1 cut(s) 1484
BspTNI GGTCTC 1 cut(s) 1142
BsrDI GCAATG 2 cut(s) 382, 1854
BsrFI RCCGGY 1 cut(s) 2421
BsrI ACTGG 5 cut(s) 62, 428, 1504, 1757, 2323
BssAI RCCGGY 1 cut(s) 2421
BssECI CCNNGG 9 cut(s) 614, 615, 693, 943, 1330, 1360, 1471, 1975, 1976
BssMI GATC 8 cut(s) 18, 90, 182, 232, 625, 818, 1124, 1792
BssNI GRCGYC 1 cut(s) 1607
BssT1I CCWWGG 4 cut(s) 943, 1330, 1360, 1471
Bst2UI CCWGG 4 cut(s) 1392, 1521, 1977, 2478
Bst4CI ACNGT 6 cut(s) 337, 534, 748, 871, 892, 2382
Bst6I CTCTTC 1 cut(s) 455
BstACI GRCGYC 1 cut(s) 1607
BstAPI GCANNNNNTGC 4 cut(s) 1265, 2026, 2035, 2356
BstC8I GCNNGC 3 cut(s) 1093, 1743, 1951
BstENI CCTNNNNNAGG 1 cut(s) 942
BstFNI CGCG 1 cut(s) 1095
BstHHI GCGC 1 cut(s) 609
BstKTI GATC 8 cut(s) 21, 93, 185, 235, 628, 821, 1127, 1795
BstMAI GTCTC 6 cut(s) 76, 1142, 1533, 1669, 1730, 1920
BstMBI GATC 8 cut(s) 18, 90, 182, 232, 625, 818, 1124, 1792
BstNI CCWGG 4 cut(s) 1392, 1521, 1977, 2478
BstNSI RCATGY 3 cut(s) 929, 1175, 1528
BstSCI CCNGG 8 cut(s) 614, 615, 692, 693, 1390, 1519, 1975, 2476
BstSFI CTRYAG 9 cut(s) 433, 1026, 1131, 1619, 1725, 2105, 2156, 2348, 2357
BstUI CGCG 1 cut(s) 1095
BstX2I RGATCY 4 cut(s) 182, 625, 818, 1124
BstYI RGATCY 4 cut(s) 182, 625, 818, 1124
BsuI GTATCC 1 cut(s) 1706
BtsIMutI CAGTG 5 cut(s) 55, 539, 783, 876, 888
BveI ACCTGC 2 cut(s) 260, 922
Cac8I GCNNGC 3 cut(s) 1093, 1743, 1951
CaiI CAGNNNCTG 3 cut(s) 845, 1733, 2356
CfoI GCGC 1 cut(s) 609
Cfr10I RCCGGY 1 cut(s) 2421
Cfr13I GGNCC 7 cut(s) 50, 691, 785, 911, 1244, 1418, 2465
Cfr9I CCCGGG 2 cut(s) 615, 693
CseI GACGC 1 cut(s) 1747
Csp6I GTAC 5 cut(s) 203, 720, 744, 1302, 1485
CspCI CAANNNNNGTGG 2 cut(s) 127, 162
CviQI GTAC 5 cut(s) 203, 720, 744, 1302, 1485
DpnI GATC 8 cut(s) 20, 92, 184, 234, 627, 820, 1126, 1794
DpnII GATC 8 cut(s) 18, 90, 182, 232, 625, 818, 1124, 1792
DraIII CACNNNGTG 1 cut(s) 1562
EaeI YGGCCR 5 cut(s) 42, 833, 1164, 1378, 1754
Eam1104I CTCTTC 1 cut(s) 455
EarI CTCTTC 1 cut(s) 455
Eco130I CCWWGG 4 cut(s) 943, 1330, 1360, 1471
Eco31I GGTCTC 1 cut(s) 1142
Eco32I GATATC 1 cut(s) 1188
Eco47I GGWCC 2 cut(s) 911, 1418
Eco57I CTGAAG 3 cut(s) 333, 1224, 1281
Eco88I CYCGRG 2 cut(s) 615, 693
EcoNI CCTNNNNNAGG 1 cut(s) 942
EcoO109I RGGNCCY 3 cut(s) 1244, 1418, 2465
EcoRI GAATTC 1 cut(s) 1102
EcoRII CCWGG 4 cut(s) 1390, 1519, 1975, 2476
EcoRV GATATC 1 cut(s) 1188
EcoT14I CCWWGG 4 cut(s) 943, 1330, 1360, 1471
EcoT22I ATGCAT 1 cut(s) 1173
ErhI CCWWGG 4 cut(s) 943, 1330, 1360, 1471
Esp3I CGTCTC 3 cut(s) 76, 1730, 1920
FalI AAGNNNNNCTT 2 cut(s) 709, 741
FaqI GGGAC 2 cut(s) 1404, 1516
FauI CCCGC 1 cut(s) 592
FspBI CTAG 3 cut(s) 402, 1701, 1835
GlaI GCGC 1 cut(s) 608
GsuI CTGGAG 5 cut(s) 411, 1140, 1503, 2072, 2306
HapII CCGG 5 cut(s) 192, 616, 694, 714, 2422
HgaI GACGC 1 cut(s) 1747
HhaI GCGC 1 cut(s) 609
Hin1I GRCGYC 1 cut(s) 1607
Hin6I GCGC 1 cut(s) 607
HinP1I GCGC 1 cut(s) 607
HincII GTYRAC 1 cut(s) 850
HindII GTYRAC 1 cut(s) 850
HindIII AAGCTT 3 cut(s) 1889, 1947, 2406
HinfI GANTC 5 cut(s) 829, 1399, 1450, 1510, 1852
HpaI GTTAAC 1 cut(s) 850
HpaII CCGG 5 cut(s) 192, 616, 694, 714, 2422
HphI GGTGA 2 cut(s) 963, 995
Hpy166II GTNNAC 1 cut(s) 850
Hpy188III TCNNGA 7 cut(s) 85, 108, 714, 1119, 1317, 1494, 1790
Hpy8I GTNNAC 1 cut(s) 850
Hpy99I CGWCG 2 cut(s) 71, 86
HpyAV CCTTC 4 cut(s) 26, 296, 948, 1815
HpyCH4III ACNGT 6 cut(s) 337, 534, 748, 871, 892, 2382
HpyCH4IV ACGT 3 cut(s) 81, 1054, 1607
HpySE526I ACGT 3 cut(s) 81, 1054, 1607
Hsp92I GRCGYC 1 cut(s) 1607
HspAI GCGC 1 cut(s) 607
Kpn2I TCCGGA 1 cut(s) 713
KpnI GGTACC 1 cut(s) 1488
KspAI GTTAAC 1 cut(s) 850
Kzo9I GATC 8 cut(s) 18, 90, 182, 232, 625, 818, 1124, 1792
LmnI GCTCC 4 cut(s) 769, 880, 2091, 2325
MaeI CTAG 3 cut(s) 402, 1701, 1835
MaeII ACGT 3 cut(s) 81, 1054, 1607
MaeIII GTNAC 9 cut(s) 163, 404, 778, 1039, 1352, 1554, 1596, 2325, 2424
MalI GATC 8 cut(s) 20, 92, 184, 234, 627, 820, 1126, 1794
MboI GATC 8 cut(s) 18, 90, 182, 232, 625, 818, 1124, 1792
MboII GAAGA 7 cut(s) 100, 472, 960, 969, 1119, 1197, 1230
MfeI CAATTG 2 cut(s) 294, 1907
MflI RGATCY 4 cut(s) 182, 625, 818, 1124
MhlI GDGCHC 2 cut(s) 1277, 1618
MlsI TGGCCA 3 cut(s) 1166, 1380, 1756
MluCI AATT 8 cut(s) 294, 444, 679, 1102, 1321, 1907, 2337, 2429
MluI ACGCGT 1 cut(s) 1093
MluNI TGGCCA 3 cut(s) 1166, 1380, 1756
MlyI GAGTC 2 cut(s) 1408, 1444
MmeI TCCRAC 3 cut(s) 197, 818, 977
Mox20I TGGCCA 3 cut(s) 1166, 1380, 1756
Mph1103I ATGCAT 1 cut(s) 1173
MroI TCCGGA 1 cut(s) 713
MscI TGGCCA 3 cut(s) 1166, 1380, 1756
MseI TTAA 5 cut(s) 119, 704, 849, 1995, 2497
MslI CAYNNNNRTG 1 cut(s) 1445
Msp20I TGGCCA 3 cut(s) 1166, 1380, 1756
MspI CCGG 5 cut(s) 192, 616, 694, 714, 2422
MspR9I CCNGG 8 cut(s) 616, 617, 694, 695, 1392, 1521, 1977, 2478
MunI CAATTG 2 cut(s) 294, 1907
Mva1269I GAATGC 2 cut(s) 387, 1971
MvaI CCWGG 4 cut(s) 1392, 1521, 1977, 2478
MvnI CGCG 1 cut(s) 1095
NciI CCSGG 4 cut(s) 616, 617, 694, 695
NdeII GATC 8 cut(s) 18, 90, 182, 232, 625, 818, 1124, 1792
NlaIV GGNNCC 3 cut(s) 882, 1420, 1486
NmuCI GTSAC 7 cut(s) 163, 778, 1039, 1554, 1596, 2325, 2424
NsiI ATGCAT 1 cut(s) 1173
NspI RCATGY 3 cut(s) 929, 1175, 1528
PaqCI CACCTGC 1 cut(s) 260
PasI CCCWGGG 1 cut(s) 1976
PciI ACATGT 1 cut(s) 925
PctI GAATGC 2 cut(s) 387, 1971
PfeI GAWTC 3 cut(s) 829, 1510, 1852
PflMI CCANNNNNTGG 2 cut(s) 980, 1147
PleI GAGTC 2 cut(s) 1407, 1444
PpsI GAGTC 2 cut(s) 1407, 1444
PpuMI RGGWCCY 1 cut(s) 1418
PscI ACATGT 1 cut(s) 925
Psp5II RGGWCCY 1 cut(s) 1418
Psp6I CCWGG 4 cut(s) 1390, 1519, 1975, 2476
PspGI CCWGG 4 cut(s) 1390, 1519, 1975, 2476
PspN4I GGNNCC 3 cut(s) 882, 1420, 1486
PspPI GGNCC 7 cut(s) 50, 691, 785, 911, 1244, 1418, 2465
PspPPI RGGWCCY 1 cut(s) 1418
PstI CTGCAG 5 cut(s) 1623, 2109, 2160, 2352, 2361
PstNI CAGNNNCTG 3 cut(s) 845, 1733, 2356
PsuI RGATCY 4 cut(s) 182, 625, 818, 1124
RsaI GTAC 5 cut(s) 204, 721, 745, 1303, 1486
RsaNI GTAC 5 cut(s) 203, 720, 744, 1302, 1485
RseI CAYNNNNRTG 1 cut(s) 1445
SaqAI TTAA 5 cut(s) 119, 704, 849, 1995, 2497
Sau3AI GATC 8 cut(s) 18, 90, 182, 232, 625, 818, 1124, 1792
Sau96I GGNCC 7 cut(s) 50, 691, 785, 911, 1244, 1418, 2465
ScaI AGTACT 1 cut(s) 745
SchI GAGTC 2 cut(s) 1408, 1444
ScrFI CCNGG 8 cut(s) 616, 617, 694, 695, 1392, 1521, 1977, 2478
SduI GDGCHC 2 cut(s) 1277, 1618
SfcI CTRYAG 9 cut(s) 433, 1026, 1131, 1619, 1725, 2105, 2156, 2348, 2357
SinI GGWCC 2 cut(s) 911, 1418
SmaI CCCGGG 2 cut(s) 617, 695
SmiMI CAYNNNNRTG 1 cut(s) 1445
SmlI CTYRAG 1 cut(s) 1086
SmoI CTYRAG 1 cut(s) 1086
SrfI GCCCGGGC 1 cut(s) 695
Sse9I AATT 8 cut(s) 294, 444, 679, 1102, 1321, 1907, 2337, 2429
SspI AATATT 1 cut(s) 472
SspMI CTAG 3 cut(s) 402, 1701, 1835
StyD4I CCNGG 8 cut(s) 614, 615, 692, 693, 1390, 1519, 1975, 2476
StyI CCWWGG 4 cut(s) 943, 1330, 1360, 1471
TaaI ACNGT 6 cut(s) 337, 534, 748, 871, 892, 2382
TaiI ACGT 3 cut(s) 84, 1057, 1610
TaqI TCGA 2 cut(s) 84, 1190
TasI AATT 8 cut(s) 294, 444, 679, 1102, 1321, 1907, 2337, 2429
TatI WGTACW 2 cut(s) 719, 743
TauI GCSGC 5 cut(s) 44, 790, 1112, 1956, 2053
TfiI GAWTC 3 cut(s) 829, 1510, 1852
Tru1I TTAA 5 cut(s) 119, 704, 849, 1995, 2497
Tru9I TTAA 5 cut(s) 119, 704, 849, 1995, 2497
TscAI CASTG 5 cut(s) 62, 539, 783, 876, 895
TseFI GTSAC 7 cut(s) 163, 778, 1039, 1554, 1596, 2325, 2424
Tsp45I GTSAC 7 cut(s) 163, 778, 1039, 1554, 1596, 2325, 2424
TspDTI ATGAA 8 cut(s) 842, 1115, 1224, 1278, 1467, 1814, 1865, 2442
TspGWI ACGGA 3 cut(s) 20, 223, 799
TspMI CCCGGG 2 cut(s) 615, 693
TspRI CASTG 5 cut(s) 62, 539, 783, 876, 895
Van91I CCANNNNNTGG 2 cut(s) 980, 1147
VpaK11BI GGWCC 2 cut(s) 911, 1418
XagI CCTNNNNNAGG 1 cut(s) 942
XapI RAATTY 5 cut(s) 444, 679, 1102, 1321, 2337
XceI RCATGY 3 cut(s) 929, 1175, 1528
XcmI CCANNNNNNNNNTGG 1 cut(s) 1388
XmaI CCCGGG 2 cut(s) 615, 693
XspI CTAG 3 cut(s) 402, 1701, 1835
ZraI GACGTC 1 cut(s) 1608
ZrmI AGTACT 1 cut(s) 745
Zsp2I ATGCAT 1 cut(s) 1173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.