Rw4G029940

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0005569
Physical Location & Seq
Reverse (-)
56581530 .. 56591242
9713 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G029940.1

Sequence Viewer

Length: 2520 bp
ATGACGGAGAAGCAGTTGATCGTGGTCGTTGAAGGCACGGCGGCCATCGGGCCTTACTGGTCAGCCGTCGTCTCCGATTACGTCGAGAAGATCATCAGGTCTTTCTCTGGAAACGAGTTAAGTGGTCAGAAGCCCTCCACTTCTATTGTTGAGATGTCTTTGGTCACATTCAATACTCATGGATCTTATTCCGGTTGCTTGGTACAACGGAGTGGTTGGACAAGAGATGTTGATCGTTTCTTACAATGGCTGTCAGCTATTCCCTTAGCAGGTGGTGGTTTTAGTGATGCTGCAATTGCAGAAGGGCTTTCTGAAGCCCTAATGATGTTCACTAACGTTCAAAATGGAAGCCAAAATCAACAAAATGTGGATTTTCAAAAGCATTGCATTCTTGTTGCCGCTAGTAACCCTCATCCCCTGCCCACTCCAGTCTATAGACCACAAATTCAAAATGTGGAGAAGAGTGAAAATATTGATGCACAAACCGAAAGCCGTCTGTATGATGCTGAGGCTGTTGCTAAATCATTTGCACAGTGCCTTGTTTCTCTGTCAGTCATCTGTCCGAAACAGCTTCCTAAACTCAGAGCCATTTACAATGCGGGAAAGCGCAACCCCCGGGCAGCAGATCCACCGATTGATAATGTGAAAAACCCCCACTTTCTCGTTCTAATCTCAGAGAATTTTATGGAGGCCCGGGCTACTTTAAGTCGTCCCGGAAGTACAACTTTGCCCTCAAATCCAAGTACTGTAAAGATGGACATAGCTCCTGTTGCATCAGTGACAGGGCCACCTCCAACTTCTATGCCATCTGTGAATGGATCTGTTATGAATCGGCCATCAGTTTCTGTTAACAATGTGCCTCCTGCGACAGTGAAAGTGGAGCCAAGCACTGTAAGTTCAATGGTAGCTGGACCTCCTTTTGCACACGTTCCATCTGTTCGTCCTCCTTCCCAAGGAGTTCCAACCTTGCAAACTTCTTCACCATCTTCTGGTGAGATGAATACAAACAATGAGAGTGTGCCAGATTTGAAACCTATAGTGAGTAATGTGACACATCAACTACGTCCTGTGGGTAGCATCCTCAATAATATCTCTCAAGCACGCGTGATGAATTCTGCAGCCTTATCTGGAGGATCTTCTATAGGTCTCCAAAACATGGGTCAAAATCCTATGGCCATGCATGTATCAAACATGATATCGAGTGGAATGGCATCTTCGGTGGCTGCTGCTCAAAATGTATTTTCATCTTCAGGGCCTCACACTCTTGCACAGGTTGCTCAGAGCACAGGTCTTGGTTCATTTACTTCAGGTACTTCCAATGTGTCTGGAAATTGTAACCTTGGGATGTCGCAACCAATGAGTAACCTCCAAGGTGGTGTTAGCATTGGCCAATCTGTTCCTGGCATGAGTCAAGGAAATCTCTCAGGACCCCAGATGGTTCAAAGTGGCATTGGAATGACTCCAAACATGATGAGTGGCCTTGGTTCATCAGGTACCTCCTCTGGAACTGGGACAATGATTCCCACTCCAGGCATGTCTCAACAGGCACAATCTGGGATGCAGTCACTTGGTGCAAACAATAGTTCAGTTTCTAATGTGCCGTTGTCACAACAGACGTCAAGTGCTCTGCAGTCTGCACAGTCCAAATATGTGAAAGTGTGGGAGGGAAACTTATCTGGGCAGAGACAAGGGCAACCTGTGTTTATTACTAGATTGGAGGGATACAGAAGTGCTACAGCATCTGAGACGCTTGCAGCAAACTGGCCACCGACAATGCAAATAGTCAGGCTCATATCTCAGGATCACATGAATAACAAGCAATATGTGGGAAGGGCAGACTTTCTAGTATTTCGGGCAATGAATCAGCATGGATTTCTTGGGCAGTTGCAAGAAAAGAAGCTTTGTGCTGTCATCCAATTGCCGTCTCAGACACTACTGCTCTCTGTTTCGGACAAAGCTTGCCGCTTGATTGGAATGCTCTTCCCAGGGGATATGGTTGTGTTTAAGCCACAAGTAGGCGGTCAGCAGCAGCAGATGCAACAAATGCAACAACAGCAGCCGCAGCTACAACAGATGCAGCAACAGCAACCGCAGCTCCAGCAACAACAGCAACCGCAACTGCAGCAGCAGCAGCATCCGCAAATGCAACAACAGCAACAGCAACCGCAACTGCAGCAGCAGCAGCATCCGCAGATGCAACAACAGCAGCAGCATCCACAGATGCAACAGCAGCAGCATCCACAGATGCAACAGCAGCAGCAACAGCAGCAGCAGCAGCAGCTTCCGCAGATGCAACAACAGCAGCAACATCCTCAGATGCAACAGCAGCAGCTCTCGCAGCTCCAGTCACAACAGCAAATTCCACAACTGCAGCAACTGCAGCAACAACACCAACAGCAACAGTTGGTTGGGGGTGGAATGGGTCAAGCTTATGGTCAAGGCCGGTCACAATTACTTTCACAAGGACAAGTTTCATCACAACAAGGGCCTAATATGCCCGGAGGAGGCTTTATGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med25_VWA

Protein Analysis

839

Amino Acids

90.48

Weight (kDa)

9.08

Isoelectric Point (pI)

61.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med25_VWA PF11265 3 - 226 2e-44 Mediator complex subunit 25 von Willebrand factor type A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 260
AatII GACGTC 1 cut(s) 1619
Acc36I ACCTGC 1 cut(s) 260
Acc65I GGTACC 1 cut(s) 1493
AccB1I GGYRCC 1 cut(s) 1493
AccB7I CCANNNNNTGG 2 cut(s) 989, 1156
AccII CGCG 1 cut(s) 1104
AclI AACGTT 1 cut(s) 336
AclWI GGATC 5 cut(s) 190, 620, 826, 1141, 1809
AcoI YGGCCR 5 cut(s) 42, 833, 1173, 1387, 1763
AcsI RAATTY 4 cut(s) 444, 679, 1111, 2358
AcuI CTGAAG 3 cut(s) 333, 1233, 1290
AcyI GRCGYC 1 cut(s) 1616
AdeI CACNNNGTG 1 cut(s) 1571
AfaI GTAC 5 cut(s) 204, 721, 745, 1312, 1495
AfiI CCNNNNNNNGG 7 cut(s) 269, 953, 989, 1156, 1529, 2015, 2504
AflIII ACRYGT 2 cut(s) 925, 1102
AgsI TTSAA 8 cut(s) 32, 172, 341, 377, 449, 900, 1030, 1442
AjnI CCWGG 3 cut(s) 1399, 1528, 1984
Alw21I GWGCWC 2 cut(s) 1286, 1627
Alw26I GTCTC 6 cut(s) 76, 1151, 1542, 1678, 1739, 1929
AlwI GGATC 5 cut(s) 190, 620, 826, 1141, 1809
AlwNI CAGNNNCTG 3 cut(s) 845, 1742, 2377
Ama87I CYCGRG 2 cut(s) 615, 693
ApoI RAATTY 4 cut(s) 444, 679, 1111, 2358
ArsI GACNNNNNNTTYG 2 cut(s) 1144, 1176
Asp718I GGTACC 1 cut(s) 1493
AspLEI GCGC 1 cut(s) 609
AspS9I GGNCC 7 cut(s) 50, 691, 785, 911, 1253, 1427, 2486
AsuC2I CCSGG 6 cut(s) 616, 617, 694, 695, 714, 2499
AsuHPI GGTGA 2 cut(s) 972, 1004
AvaI CYCGRG 2 cut(s) 615, 693
AvaII GGWCC 2 cut(s) 911, 1427
BaeI ACNNNNGTAYC 2 cut(s) 1714, 1747
BalI TGGCCA 3 cut(s) 1175, 1389, 1765
BanI GGYRCC 1 cut(s) 1493
Bbv12I GWGCWC 2 cut(s) 1286, 1627
BbvCI CCTCAGC 1 cut(s) 507
BccI CCATC 7 cut(s) 53, 748, 814, 844, 940, 991, 1429
BceAI ACGGC 5 cut(s) 50, 54, 477, 1585, 1906
BciT130I CCWGG 3 cut(s) 1401, 1530, 1986
BciVI GTATCC 1 cut(s) 1715
BcnI CCSGG 6 cut(s) 616, 617, 694, 695, 714, 2499
BcoDI GTCTC 6 cut(s) 76, 1151, 1542, 1678, 1739, 1929
BfaI CTAG 3 cut(s) 402, 1710, 1844
BfuAI ACCTGC 1 cut(s) 260
BfuI GTATCC 1 cut(s) 1715
BmcAI AGTACT 1 cut(s) 745
Bme1390I CCNGG 9 cut(s) 616, 617, 694, 695, 714, 1401, 1530, 1986, 2499
Bme18I GGWCC 2 cut(s) 911, 1427
BmeT110I CYCGRG 2 cut(s) 615, 693
BmgT120I GGNCC 7 cut(s) 50, 691, 785, 911, 1253, 1427, 2486
BmiI GGNNCC 3 cut(s) 882, 1429, 1495
BmrFI CCNGG 9 cut(s) 616, 617, 694, 695, 714, 1401, 1530, 1986, 2499
BmrI ACTGGG 1 cut(s) 1518
BmuI ACTGGG 1 cut(s) 1518
BpmI CTGGAG 5 cut(s) 411, 1149, 1512, 2081, 2327
Bpu10I CCTNAGC 2 cut(s) 265, 507
BpuEI CTTGAG 1 cut(s) 1080
BpuMI CCSGG 6 cut(s) 616, 617, 694, 695, 714, 2499
BsaBI GATNNNNATC 1 cut(s) 231
BsaHI GRCGYC 1 cut(s) 1616
BsaI GGTCTC 1 cut(s) 1151
BsaJI CCNNGG 9 cut(s) 614, 615, 693, 952, 1339, 1369, 1480, 1984, 1985
BsaWI WCCGGW 1 cut(s) 191
BsaXI ACNNNNNCTCC 3 cut(s) 29, 2079, 2109
Bsc4I CCNNNNNNNGG 7 cut(s) 269, 953, 989, 1156, 1529, 2015, 2504
Bse118I RCCGGY 1 cut(s) 2442
Bse1I ACTGG 5 cut(s) 62, 428, 1513, 1766, 2344
Bse3DI GCAATG 2 cut(s) 382, 1863
Bse8I GATNNNNATC 1 cut(s) 231
BseBI CCWGG 3 cut(s) 1401, 1530, 1986
BseDI CCNNGG 9 cut(s) 614, 615, 693, 952, 1339, 1369, 1480, 1984, 1985
BseJI GATNNNNATC 1 cut(s) 231
BseLI CCNNNNNNNGG 7 cut(s) 269, 953, 989, 1156, 1529, 2015, 2504
BseMI GCAATG 2 cut(s) 382, 1863
BseMII CTCAG 9 cut(s) 498, 595, 687, 1292, 1437, 1734, 1811, 1940, 2327
BseNI ACTGG 5 cut(s) 62, 428, 1513, 1766, 2344
BseRI GAGGAG 2 cut(s) 1489, 2517
BsgI GTGCAG 1 cut(s) 1620
Bsh1236I CGCG 1 cut(s) 1104
BshNI GGYRCC 1 cut(s) 1493
BsiHKAI GWGCWC 2 cut(s) 1286, 1627
BsiHKCI CYCGRG 2 cut(s) 615, 693
BsiSI CCGG 6 cut(s) 192, 616, 694, 714, 2443, 2499
BslFI GGGAC 2 cut(s) 696, 1525
BslI CCNNNNNNNGG 7 cut(s) 269, 953, 989, 1156, 1529, 2015, 2504
BsmAI GTCTC 6 cut(s) 76, 1151, 1542, 1678, 1739, 1929
BsmBI CGTCTC 3 cut(s) 76, 1739, 1929
BsmFI GGGAC 2 cut(s) 696, 1525
BsmI GAATGC 2 cut(s) 387, 1980
Bso31I GGTCTC 1 cut(s) 1151
BsoBI CYCGRG 2 cut(s) 615, 693
Bsp1286I GDGCHC 2 cut(s) 1286, 1627
Bsp143I GATC 8 cut(s) 18, 90, 182, 232, 625, 818, 1133, 1801
BspCNI CTCAG 9 cut(s) 499, 594, 686, 1291, 1436, 1735, 1810, 1939, 2326
BspFNI CGCG 1 cut(s) 1104
BspLI GGNNCC 3 cut(s) 882, 1429, 1495
BspMAI CTGCAG 6 cut(s) 1120, 1632, 2124, 2175, 2373, 2382
BspMI ACCTGC 1 cut(s) 260
BspPI GGATC 5 cut(s) 190, 620, 826, 1141, 1809
BspQI GCTCTTC 1 cut(s) 1985
BspT107I GGYRCC 1 cut(s) 1493
BspTNI GGTCTC 1 cut(s) 1151
BsrDI GCAATG 2 cut(s) 382, 1863
BsrFI RCCGGY 1 cut(s) 2442
BsrI ACTGG 5 cut(s) 62, 428, 1513, 1766, 2344
BssAI RCCGGY 1 cut(s) 2442
BssECI CCNNGG 9 cut(s) 614, 615, 693, 952, 1339, 1369, 1480, 1984, 1985
BssMI GATC 8 cut(s) 18, 90, 182, 232, 625, 818, 1133, 1801
BssNI GRCGYC 1 cut(s) 1616
BssT1I CCWWGG 4 cut(s) 952, 1339, 1369, 1480
Bst2UI CCWGG 3 cut(s) 1401, 1530, 1986
Bst4CI ACNGT 6 cut(s) 534, 748, 871, 892, 1641, 2403
Bst6I CTCTTC 2 cut(s) 455, 1985
BstACI GRCGYC 1 cut(s) 1616
BstAPI GCANNNNNTGC 4 cut(s) 1274, 2035, 2044, 2377
BstC8I GCNNGC 3 cut(s) 1102, 1752, 1960
BstENI CCTNNNNNAGG 1 cut(s) 951
BstFNI CGCG 1 cut(s) 1104
BstHHI GCGC 1 cut(s) 609
BstKTI GATC 8 cut(s) 21, 93, 185, 235, 628, 821, 1136, 1804
BstMAI GTCTC 6 cut(s) 76, 1151, 1542, 1678, 1739, 1929
BstMBI GATC 8 cut(s) 18, 90, 182, 232, 625, 818, 1133, 1801
BstNI CCWGG 3 cut(s) 1401, 1530, 1986
BstNSI RCATGY 2 cut(s) 1184, 1537
BstSCI CCNGG 9 cut(s) 614, 615, 692, 693, 712, 1399, 1528, 1984, 2497
BstUI CGCG 1 cut(s) 1104
BstX2I RGATCY 4 cut(s) 182, 625, 818, 1133
BstYI RGATCY 4 cut(s) 182, 625, 818, 1133
BsuI GTATCC 1 cut(s) 1715
BtsIMutI CAGTG 4 cut(s) 539, 783, 876, 888
BveI ACCTGC 1 cut(s) 260
Cac8I GCNNGC 3 cut(s) 1102, 1752, 1960
CaiI CAGNNNCTG 3 cut(s) 845, 1742, 2377
CfoI GCGC 1 cut(s) 609
Cfr10I RCCGGY 1 cut(s) 2442
Cfr13I GGNCC 7 cut(s) 50, 691, 785, 911, 1253, 1427, 2486
Cfr9I CCCGGG 2 cut(s) 615, 693
CseI GACGC 1 cut(s) 1756
Csp6I GTAC 5 cut(s) 203, 720, 744, 1311, 1494
CspCI CAANNNNNGTGG 2 cut(s) 127, 162
CviQI GTAC 5 cut(s) 203, 720, 744, 1311, 1494
DpnI GATC 8 cut(s) 20, 92, 184, 234, 627, 820, 1135, 1803
DpnII GATC 8 cut(s) 18, 90, 182, 232, 625, 818, 1133, 1801
DraIII CACNNNGTG 1 cut(s) 1571
EaeI YGGCCR 5 cut(s) 42, 833, 1173, 1387, 1763
Eam1104I CTCTTC 2 cut(s) 455, 1985
EarI CTCTTC 2 cut(s) 455, 1985
Eco130I CCWWGG 4 cut(s) 952, 1339, 1369, 1480
Eco31I GGTCTC 1 cut(s) 1151
Eco32I GATATC 1 cut(s) 1197
Eco47I GGWCC 2 cut(s) 911, 1427
Eco57I CTGAAG 3 cut(s) 333, 1233, 1290
Eco88I CYCGRG 2 cut(s) 615, 693
EcoNI CCTNNNNNAGG 1 cut(s) 951
EcoO109I RGGNCCY 3 cut(s) 1253, 1427, 2486
EcoRI GAATTC 1 cut(s) 1111
EcoRII CCWGG 3 cut(s) 1399, 1528, 1984
EcoRV GATATC 1 cut(s) 1197
EcoT14I CCWWGG 4 cut(s) 952, 1339, 1369, 1480
EcoT22I ATGCAT 1 cut(s) 1182
ErhI CCWWGG 4 cut(s) 952, 1339, 1369, 1480
Esp3I CGTCTC 3 cut(s) 76, 1739, 1929
FalI AAGNNNNNCTT 2 cut(s) 709, 741
FaqI GGGAC 2 cut(s) 696, 1525
FauI CCCGC 1 cut(s) 592
FspBI CTAG 3 cut(s) 402, 1710, 1844
GlaI GCGC 1 cut(s) 608
GsuI CTGGAG 5 cut(s) 411, 1149, 1512, 2081, 2327
HapII CCGG 6 cut(s) 192, 616, 694, 714, 2443, 2499
HgaI GACGC 1 cut(s) 1756
HhaI GCGC 1 cut(s) 609
Hin1I GRCGYC 1 cut(s) 1616
Hin6I GCGC 1 cut(s) 607
HinP1I GCGC 1 cut(s) 607
HincII GTYRAC 1 cut(s) 850
HindII GTYRAC 1 cut(s) 850
HindIII AAGCTT 3 cut(s) 1898, 1956, 2427
HinfI GANTC 5 cut(s) 829, 1408, 1459, 1519, 1861
HpaI GTTAAC 1 cut(s) 850
HpaII CCGG 6 cut(s) 192, 616, 694, 714, 2443, 2499
HphI GGTGA 2 cut(s) 972, 1004
Hpy166II GTNNAC 2 cut(s) 330, 850
Hpy188III TCNNGA 7 cut(s) 85, 108, 1128, 1326, 1425, 1503, 1799
Hpy8I GTNNAC 2 cut(s) 330, 850
Hpy99I CGWCG 2 cut(s) 71, 86
HpyAV CCTTC 4 cut(s) 26, 296, 957, 1824
HpyCH4III ACNGT 6 cut(s) 534, 748, 871, 892, 1641, 2403
HpyCH4IV ACGT 5 cut(s) 81, 336, 927, 1063, 1616
HpySE526I ACGT 5 cut(s) 81, 336, 927, 1063, 1616
Hsp92I GRCGYC 1 cut(s) 1616
HspAI GCGC 1 cut(s) 607
KpnI GGTACC 1 cut(s) 1497
KspAI GTTAAC 1 cut(s) 850
Kzo9I GATC 8 cut(s) 18, 90, 182, 232, 625, 818, 1133, 1801
LguI GCTCTTC 1 cut(s) 1985
LmnI GCTCC 4 cut(s) 769, 880, 2100, 2346
MaeI CTAG 3 cut(s) 402, 1710, 1844
MaeII ACGT 5 cut(s) 81, 336, 927, 1063, 1616
MalI GATC 8 cut(s) 20, 92, 184, 234, 627, 820, 1135, 1803
MboI GATC 8 cut(s) 18, 90, 182, 232, 625, 818, 1133, 1801
MboII GAAGA 8 cut(s) 100, 472, 969, 978, 1128, 1206, 1239, 1972
MfeI CAATTG 2 cut(s) 294, 1916
MflI RGATCY 4 cut(s) 182, 625, 818, 1133
MhlI GDGCHC 2 cut(s) 1286, 1627
MlsI TGGCCA 3 cut(s) 1175, 1389, 1765
MluCI AATT 8 cut(s) 294, 444, 679, 1111, 1330, 1916, 2358, 2450
MluI ACGCGT 1 cut(s) 1102
MluNI TGGCCA 3 cut(s) 1175, 1389, 1765
MlyI GAGTC 2 cut(s) 1417, 1453
MmeI TCCRAC 3 cut(s) 197, 818, 986
Mox20I TGGCCA 3 cut(s) 1175, 1389, 1765
Mph1103I ATGCAT 1 cut(s) 1182
MscI TGGCCA 3 cut(s) 1175, 1389, 1765
MseI TTAA 5 cut(s) 119, 704, 849, 2004, 2518
MslI CAYNNNNRTG 1 cut(s) 1454
Msp20I TGGCCA 3 cut(s) 1175, 1389, 1765
MspI CCGG 6 cut(s) 192, 616, 694, 714, 2443, 2499
MspR9I CCNGG 9 cut(s) 616, 617, 694, 695, 714, 1401, 1530, 1986, 2499
MunI CAATTG 2 cut(s) 294, 1916
Mva1269I GAATGC 2 cut(s) 387, 1980
MvaI CCWGG 3 cut(s) 1401, 1530, 1986
MvnI CGCG 1 cut(s) 1104
NciI CCSGG 6 cut(s) 616, 617, 694, 695, 714, 2499
NdeII GATC 8 cut(s) 18, 90, 182, 232, 625, 818, 1133, 1801
NlaIV GGNNCC 3 cut(s) 882, 1429, 1495
NmuCI GTSAC 7 cut(s) 163, 778, 1048, 1563, 1605, 2346, 2445
NsiI ATGCAT 1 cut(s) 1182
NspI RCATGY 2 cut(s) 1184, 1537
PaqCI CACCTGC 1 cut(s) 260
PasI CCCWGGG 1 cut(s) 1985
PciSI GCTCTTC 1 cut(s) 1985
PctI GAATGC 2 cut(s) 387, 1980
PfeI GAWTC 3 cut(s) 829, 1519, 1861
PflMI CCANNNNNTGG 2 cut(s) 989, 1156
PfoI TCCNGGA 1 cut(s) 712
PleI GAGTC 2 cut(s) 1416, 1453
PpsI GAGTC 2 cut(s) 1416, 1453
PpuMI RGGWCCY 1 cut(s) 1427
Psp1406I AACGTT 1 cut(s) 336
Psp5II RGGWCCY 1 cut(s) 1427
Psp6I CCWGG 3 cut(s) 1399, 1528, 1984
PspGI CCWGG 3 cut(s) 1399, 1528, 1984
PspN4I GGNNCC 3 cut(s) 882, 1429, 1495
PspPI GGNCC 7 cut(s) 50, 691, 785, 911, 1253, 1427, 2486
PspPPI RGGWCCY 1 cut(s) 1427
PstI CTGCAG 6 cut(s) 1120, 1632, 2124, 2175, 2373, 2382
PstNI CAGNNNCTG 3 cut(s) 845, 1742, 2377
PsuI RGATCY 4 cut(s) 182, 625, 818, 1133
RsaI GTAC 5 cut(s) 204, 721, 745, 1312, 1495
RsaNI GTAC 5 cut(s) 203, 720, 744, 1311, 1494
RseI CAYNNNNRTG 1 cut(s) 1454
SapI GCTCTTC 1 cut(s) 1985
SaqAI TTAA 5 cut(s) 119, 704, 849, 2004, 2518
Sau3AI GATC 8 cut(s) 18, 90, 182, 232, 625, 818, 1133, 1801
Sau96I GGNCC 7 cut(s) 50, 691, 785, 911, 1253, 1427, 2486
ScaI AGTACT 1 cut(s) 745
SchI GAGTC 2 cut(s) 1417, 1453
ScrFI CCNGG 9 cut(s) 616, 617, 694, 695, 714, 1401, 1530, 1986, 2499
SduI GDGCHC 2 cut(s) 1286, 1627
SinI GGWCC 2 cut(s) 911, 1427
SmaI CCCGGG 2 cut(s) 617, 695
SmiMI CAYNNNNRTG 1 cut(s) 1454
SmlI CTYRAG 1 cut(s) 1095
SmoI CTYRAG 1 cut(s) 1095
SrfI GCCCGGGC 1 cut(s) 695
Sse9I AATT 8 cut(s) 294, 444, 679, 1111, 1330, 1916, 2358, 2450
SspI AATATT 1 cut(s) 472
SspMI CTAG 3 cut(s) 402, 1710, 1844
StyD4I CCNGG 9 cut(s) 614, 615, 692, 693, 712, 1399, 1528, 1984, 2497
StyI CCWWGG 4 cut(s) 952, 1339, 1369, 1480
TaaI ACNGT 6 cut(s) 534, 748, 871, 892, 1641, 2403
TaiI ACGT 5 cut(s) 84, 339, 930, 1066, 1619
TaqI TCGA 2 cut(s) 84, 1199
TasI AATT 8 cut(s) 294, 444, 679, 1111, 1330, 1916, 2358, 2450
TatI WGTACW 2 cut(s) 719, 743
TauI GCSGC 4 cut(s) 44, 401, 1965, 2062
TfiI GAWTC 3 cut(s) 829, 1519, 1861
Tru1I TTAA 5 cut(s) 119, 704, 849, 2004, 2518
Tru9I TTAA 5 cut(s) 119, 704, 849, 2004, 2518
TscAI CASTG 4 cut(s) 539, 783, 876, 895
TseFI GTSAC 7 cut(s) 163, 778, 1048, 1563, 1605, 2346, 2445
Tsp45I GTSAC 7 cut(s) 163, 778, 1048, 1563, 1605, 2346, 2445
TspDTI ATGAA 9 cut(s) 842, 1013, 1124, 1233, 1287, 1476, 1823, 1874, 2463
TspGWI ACGGA 2 cut(s) 20, 223
TspMI CCCGGG 2 cut(s) 615, 693
TspRI CASTG 4 cut(s) 539, 783, 876, 895
Van91I CCANNNNNTGG 2 cut(s) 989, 1156
VpaK11BI GGWCC 2 cut(s) 911, 1427
XagI CCTNNNNNAGG 1 cut(s) 951
XapI RAATTY 4 cut(s) 444, 679, 1111, 2358
XceI RCATGY 2 cut(s) 1184, 1537
XcmI CCANNNNNNNNNTGG 1 cut(s) 1397
XmaI CCCGGG 2 cut(s) 615, 693
XspI CTAG 3 cut(s) 402, 1710, 1844
ZraI GACGTC 1 cut(s) 1617
ZrmI AGTACT 1 cut(s) 745
Zsp2I ATGCAT 1 cut(s) 1182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.