MD16G1047200.v1.1

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0005569
Physical Location & Seq
Reverse (-)
3272501 .. 3281132
8632 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1047200.v1.1.491

Sequence Viewer

Length: 2571 bp
ATGGCGGAGAAGCAGCTGATCGTGGCCGTTGAAGGTACCGCCGCCATGGGGCCCTGCTGGGCAACCGTCGTCTCCGATTACCTTGATAAGATCATCAGGAGTTTCTGTGGAAACGAGCCAAGTGGGCAGAAGCTGTCGACTTCTACTGTTGAGCTCTCCCTGGTCACGTTCAATACTCATGGATCTTATTGTGGTTGCCTGGTACAACGGAGTGGCTGGACAAGGGATGTGAATCTCTTCTTACATAGGCTTTCAGCTATACCCTTTTCTGGGGGTGGTTTTAGTGAAGCTGCAATTGCGGAAGGGCTTTCGGAAGCTCTAATGATGTTCCCTACAGTTCAAAATGGAAGCCAAAATCAACAAAATGTGGATTGTCAGAAGCATTGTGTTCTTATTGCTGCGAGTAAACCTCACCCTCTGCCCACACCAGTGTATCGGCCACAAATTCAAAATTTGGAGCAAAATGAAAATATTGATGCACAAACAGAAAATCGTCTTTATGATGCTGAGGCTGTTGCTAAATCATTTCCACAGAGCTCTGTTTCGCTGTCAGTCATTTGTCCAATAGAGCTACCTGAGCTCAGAGCAATATACAATGCGGGAAAGCGCAACCCTAGAGCGGCAGATCCACCAATTGATAATGCGAAAAATCCCCACTTTCTTGTTTTAATCTCAGAGAATTTTATGGAGGCCCGTGCTACTTTAAGTCGTCCAGGAATTACAACTTTGCACTCAAATCAGAGTCCTGTAAAAATGGACATAGCTCCTGTTGCTTCAGTTACTGGGCCGCCTCCAACTTCTATGCCCTCTGTGAATGGACCTGTAATGAATCGGCAACCAATTTCTGTTGGAAATGTGCCTCCTGCTACTGTAAAAGTTGAGCCAAGCACTGTAAGTTCCATGGGACCTTCTTTTGCACACATTCCATCTGTCCGTCCTCCTTCCCAAGGAGTCCCGAGCTTGCAGACTTCTTCACCATCTTCTGCTTCTCAAGAGATGGCCACAAACAATGATAATGTGCCAGATTTGAAACCTGTAGTGAGTGGTGTGACACATCCCTCGCGTCCTGTAGGTAGCATCCTCAATAACATCTCTCAAGCACGCGTGATGAACGCAGCAGCCTTAACTGGAGGAACTTCTATGGGACTCCCAACTATGGGTCAAAATCCTATGGCCATGCATGTATCAAACATGATATCTAGTGGAATGGCATCTTCAGTTGGTGCTGCTCAAAATGTATTTTCATCTTCCGGGTCCGGCACTCTTACACAGGTTGCGCAGAACCCAGGTCTTAATTCATTCACTTCAGGTACCTCTAATGTCTCTGGCAACAATAACCTTGGGATGTCACAACCAATGAGCAACAATAACCTTGGGATGTCACAACCAATGAGCAACCTTCAAGGTGGTGTTAGTATGGGTCAAGCTGTGCCTGGTATGAGCCAAGGAAATCTCTCGGGACCGCAAATGGTTCAAAGTGGAATTGGCATGAACCCAAACATGATGAGTGCCCTTGGTTCAACAGGTGCCTCTTCTGGAACTGGTACAATGATTCCTACTCCAGGAATGGCTCAACAGGTGCAATCTGGGATGCAGTCACTTGGTGCAAACAATAATTCAGCATCTAATGTGCCATTTTCACAACAGACATCAAGTGCTCTGGCATCTGCACAATCCAAATATGTGAAAGTCTGGGAGGGAGCTTTATCTGGGCAGAGACAAGGGCAGCCTGTGTTTATCACTAGATTGGAGGGTTACAGGAGTGCTTCAGCTTCTGAGACGCTTGCGGCGAACTGGCCACCAACAATGCAAATAGTGAGGCTCATATCTCAGGACCACATGAATAACAAGCAATATGTTGGAAAGGCAGACTTTTTAGTTTTTCGGGCAATGAATCAGCATGGGTTTCTTGGGCAATTGCAAGAGAAGAAACTTTGTGCAGTGATCCAATTGCCCTCTCAGACACTACTGCTCTCTGTTTCAGACAAAGCATATCGCTTGATTGGAATGCTGTTTCCAGGGGATATGGTTGTGTTTAAACCACAAGTAGGTGGTCAGCAACAGCAGCAGCAGCAGCAGCAGCAGCAAATGCAACAGCTACAACAGCAACAAATTCAAGTAGGCGGTCAGCAACAACACATGCAAGTTCCTGGTCAGCAACAGCAGCAACAAATTCAAGTAGGCGGTCAGCAACAACACATGCAAGTTCCCGGTCAGCAACAGCAGCAACAAATGCAAGTACCGGGTCAGCAACAGCAGCAACAAATGCAAGTACCCGGTCAGCAACAACAGCATCCTCAGATTCAACAACAACATCCACAGATTCAACATCAGCACCCTCAGATTCAACAACAGCAGCAACTCTCGCAGCAGCAGCTCTCACAACTTCAATCACAACAGCAACTGCCGCAGCTTCAGCAATTGCAGCAACAGCATCAACAGCATCAACAACAACAGTTGGTTGGGACGGGGATGACTCAAGCTTATGTTCAAGGCCGGTCGCAATTAGTTTCACAGGGACAGGTTTCGTCACAAGGGCCTAATATGCCTGGAGGGGGCTTTATGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med25_VWA

Protein Analysis

857

Amino Acids

91.67

Weight (kDa)

8.7

Isoelectric Point (pI)

56.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med25_VWA PF11265 3 - 226 5.7e-41 Mediator complex subunit 25 von Willebrand factor type A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1278
Acc65I GGTACC 2 cut(s) 35, 1310
AccB1I GGYRCC 3 cut(s) 35, 1310, 1526
AccBSI CCGCTC 1 cut(s) 620
AccI GTMKAC 1 cut(s) 137
AccII CGCG 2 cut(s) 1063, 1104
AclWI GGATC 3 cut(s) 190, 620, 1939
AcoI YGGCCR 5 cut(s) 24, 437, 999, 1173, 1796
AcsI RAATTY 5 cut(s) 444, 451, 679, 2112, 2172
AcuI CTGAAG 5 cut(s) 759, 1200, 1290, 1752, 2399
AdeI CACNNNGTG 1 cut(s) 1604
AfaI GTAC 6 cut(s) 37, 204, 1312, 1546, 2241, 2274
AflIII ACRYGT 1 cut(s) 1102
AjnI CCWGG 9 cut(s) 159, 198, 712, 1285, 1432, 1561, 2017, 2149, 2548
AleI CACNNNNGTG 1 cut(s) 428
Alw21I GWGCWC 4 cut(s) 156, 539, 582, 1660
Alw26I GTCTC 4 cut(s) 76, 1327, 1711, 1772
AlwI GGATC 3 cut(s) 190, 620, 1939
AlwNI CAGNNNCTG 4 cut(s) 133, 782, 1775, 2404
Ama87I CYCGRG 2 cut(s) 955, 1456
ApaI GGGCCC 1 cut(s) 54
ApoI RAATTY 5 cut(s) 444, 451, 679, 2112, 2172
Asp700I GAANNNNTTC 1 cut(s) 236
Asp718I GGTACC 2 cut(s) 35, 1310
AspLEI GCGC 2 cut(s) 609, 1279
AsuC2I CCSGG 4 cut(s) 1252, 2211, 2244, 2277
AsuHPI GGTGA 2 cut(s) 404, 966
AvaI CYCGRG 2 cut(s) 955, 1456
AvaII GGWCC 5 cut(s) 818, 905, 1254, 1460, 1834
BaeGI GKGCMC 2 cut(s) 54, 1513
BaeI ACNNNNGTAYC 2 cut(s) 416, 449
BalI TGGCCA 3 cut(s) 1001, 1175, 1798
BanI GGYRCC 3 cut(s) 35, 1310, 1526
BanII GRGCYC 4 cut(s) 54, 156, 539, 582
Bbv12I GWGCWC 4 cut(s) 156, 539, 582, 1660
BbvCI CCTCAGC 1 cut(s) 507
BccI CCATC 3 cut(s) 934, 985, 991
BceAI ACGGC 1 cut(s) 11
BciT130I CCWGG 9 cut(s) 161, 200, 714, 1287, 1434, 1563, 2019, 2151, 2550
BcnI CCSGG 4 cut(s) 1252, 2211, 2244, 2277
BcoDI GTCTC 4 cut(s) 76, 1327, 1711, 1772
BfaI CTAG 3 cut(s) 615, 1200, 1743
BfmI CTRYAG 3 cut(s) 333, 1035, 1068
BglI GCCNNNNNGGC 1 cut(s) 124
Bme18I GGWCC 5 cut(s) 818, 905, 1254, 1460, 1834
BmeT110I CYCGRG 2 cut(s) 955, 1456
BmiI GGNNCC 8 cut(s) 37, 51, 52, 906, 1255, 1312, 1461, 1528
BmrI ACTGGG 1 cut(s) 792
BmuI ACTGGG 1 cut(s) 792
BplI GAGNNNNNCTC 2 cut(s) 394, 426
BpmI CTGGAG 3 cut(s) 1149, 1545, 2571
Bpu10I CCTNAGC 2 cut(s) 507, 576
BpuEI CTTGAG 3 cut(s) 975, 1080, 2463
BpuMI CCSGG 4 cut(s) 1252, 2211, 2244, 2277
BsaBI GATNNNNATC 1 cut(s) 231
BsaXI ACNNNNNCTCC 2 cut(s) 91, 121
Bse118I RCCGGY 1 cut(s) 2496
Bse1I ACTGG 5 cut(s) 428, 787, 1132, 1546, 1799
Bse3DI GCAATG 1 cut(s) 1896
Bse8I GATNNNNATC 1 cut(s) 231
BseBI CCWGG 9 cut(s) 161, 200, 714, 1287, 1434, 1563, 2019, 2151, 2550
BseGI GGATG 9 cut(s) 232, 1054, 1077, 1350, 1383, 1596, 2293, 2314, 2478
BseJI GATNNNNATC 1 cut(s) 231
BseMI GCAATG 1 cut(s) 1896
BseMII CTCAG 9 cut(s) 498, 567, 595, 687, 1767, 1844, 1973, 2312, 2354
BseNI ACTGG 5 cut(s) 428, 787, 1132, 1546, 1799
BseSI GKGCMC 2 cut(s) 54, 1513
BseYI CCCAGC 1 cut(s) 57
BsgI GTGCAG 2 cut(s) 1653, 1959
Bsh1236I CGCG 2 cut(s) 1063, 1104
Bsh1285I CGRYCG 1 cut(s) 2501
BshNI GGYRCC 3 cut(s) 35, 1310, 1526
BsiEI CGRYCG 1 cut(s) 2501
BsiHKAI GWGCWC 4 cut(s) 156, 539, 582, 1660
BsiHKCI CYCGRG 2 cut(s) 955, 1456
BsiSI CCGG 6 cut(s) 1251, 1257, 2211, 2243, 2277, 2497
BslFI GGGAC 6 cut(s) 918, 938, 1158, 1473, 2479, 2532
BsmAI GTCTC 4 cut(s) 76, 1327, 1711, 1772
BsmBI CGTCTC 2 cut(s) 76, 1772
BsmFI GGGAC 6 cut(s) 918, 938, 1158, 1473, 2479, 2532
BsmI GAATGC 1 cut(s) 2013
BsoBI CYCGRG 2 cut(s) 955, 1456
Bsp120I GGGCCC 1 cut(s) 50
Bsp1286I GDGCHC 6 cut(s) 54, 156, 539, 582, 1513, 1660
Bsp143I GATC 5 cut(s) 18, 90, 182, 625, 1944
Bsp19I CCATGG 2 cut(s) 45, 900
BspCNI CTCAG 9 cut(s) 499, 568, 594, 686, 1768, 1843, 1972, 2311, 2353
BspFNI CGCG 2 cut(s) 1063, 1104
BspLI GGNNCC 8 cut(s) 37, 51, 52, 906, 1255, 1312, 1461, 1528
BspPI GGATC 3 cut(s) 190, 620, 1939
BspT107I GGYRCC 3 cut(s) 35, 1310, 1526
BsrBI CCGCTC 1 cut(s) 620
BsrDI GCAATG 1 cut(s) 1896
BsrFI RCCGGY 1 cut(s) 2496
BsrI ACTGG 5 cut(s) 428, 787, 1132, 1546, 1799
BssAI RCCGGY 1 cut(s) 2496
BssMI GATC 5 cut(s) 18, 90, 182, 625, 1944
BssT1I CCWWGG 7 cut(s) 45, 900, 946, 1339, 1372, 1444, 1513
Bst2UI CCWGG 9 cut(s) 161, 200, 714, 1287, 1434, 1563, 2019, 2151, 2550
Bst4CI ACNGT 6 cut(s) 67, 148, 337, 871, 892, 2457
Bst6I CTCTTC 2 cut(s) 242, 1537
BstAPI GCANNNNNTGC 3 cut(s) 2089, 2233, 2266
BstC8I GCNNGC 3 cut(s) 962, 1102, 1785
BstDEI CTNAG 9 cut(s) 507, 576, 581, 673, 1776, 1830, 1959, 2298, 2340
BstDSI CCRYGG 2 cut(s) 45, 900
BstENI CCTNNNNNAGG 2 cut(s) 945, 1560
BstF5I GGATG 9 cut(s) 232, 1054, 1077, 1350, 1383, 1596, 2293, 2314, 2478
BstFNI CGCG 2 cut(s) 1063, 1104
BstHHI GCGC 2 cut(s) 609, 1279
BstKTI GATC 5 cut(s) 21, 93, 185, 628, 1947
BstMAI GTCTC 4 cut(s) 76, 1327, 1711, 1772
BstMBI GATC 5 cut(s) 18, 90, 182, 625, 1944
BstMCI CGRYCG 1 cut(s) 2501
BstNI CCWGG 9 cut(s) 161, 200, 714, 1287, 1434, 1563, 2019, 2151, 2550
BstNSI RCATGY 3 cut(s) 1184, 2143, 2203
BstSFI CTRYAG 3 cut(s) 333, 1035, 1068
BstSLI GKGCMC 2 cut(s) 54, 1513
BstUI CGCG 2 cut(s) 1063, 1104
BstX2I RGATCY 2 cut(s) 182, 625
BstYI RGATCY 2 cut(s) 182, 625
BtgI CCRYGG 2 cut(s) 45, 900
BtsCI GGATG 9 cut(s) 232, 1054, 1077, 1350, 1383, 1596, 2293, 2314, 2478
BtsI GCAGTG 1 cut(s) 1947
BtsIMutI CAGTG 3 cut(s) 435, 888, 1947
Cac8I GCNNGC 3 cut(s) 962, 1102, 1785
CaiI CAGNNNCTG 4 cut(s) 133, 782, 1775, 2404
CfoI GCGC 2 cut(s) 609, 1279
Cfr10I RCCGGY 1 cut(s) 2496
CseI GACGC 2 cut(s) 1052, 1789
Csp6I GTAC 6 cut(s) 36, 203, 1311, 1545, 2240, 2273
CviQI GTAC 6 cut(s) 36, 203, 1311, 1545, 2240, 2273
DdeI CTNAG 9 cut(s) 507, 576, 581, 673, 1776, 1830, 1959, 2298, 2340
DpnI GATC 5 cut(s) 20, 92, 184, 627, 1946
DpnII GATC 5 cut(s) 18, 90, 182, 625, 1944
DraI TTTAAA 1 cut(s) 2038
DraIII CACNNNGTG 1 cut(s) 1604
EaeI YGGCCR 5 cut(s) 24, 437, 999, 1173, 1796
Eam1104I CTCTTC 2 cut(s) 242, 1537
EarI CTCTTC 2 cut(s) 242, 1537
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 3 cut(s) 154, 537, 580
Eco130I CCWWGG 7 cut(s) 45, 900, 946, 1339, 1372, 1444, 1513
Eco24I GRGCYC 4 cut(s) 54, 156, 539, 582
Eco32I GATATC 1 cut(s) 1197
Eco47I GGWCC 5 cut(s) 818, 905, 1254, 1460, 1834
Eco53kI GAGCTC 3 cut(s) 154, 537, 580
Eco57I CTGAAG 5 cut(s) 759, 1200, 1290, 1752, 2399
Eco88I CYCGRG 2 cut(s) 955, 1456
EcoICRI GAGCTC 3 cut(s) 154, 537, 580
EcoNI CCTNNNNNAGG 2 cut(s) 945, 1560
EcoO109I RGGNCCY 4 cut(s) 50, 51, 905, 2537
EcoRII CCWGG 9 cut(s) 159, 198, 712, 1285, 1432, 1561, 2017, 2149, 2548
EcoRV GATATC 1 cut(s) 1197
EcoT14I CCWWGG 7 cut(s) 45, 900, 946, 1339, 1372, 1444, 1513
EcoT22I ATGCAT 1 cut(s) 1182
EcoT38I GRGCYC 4 cut(s) 54, 156, 539, 582
ErhI CCWWGG 7 cut(s) 45, 900, 946, 1339, 1372, 1444, 1513
Esp3I CGTCTC 2 cut(s) 76, 1772
FalI AAGNNNNNCTT 2 cut(s) 1856, 1888
FaqI GGGAC 6 cut(s) 918, 938, 1158, 1473, 2479, 2532
FauI CCCGC 1 cut(s) 592
FblI GTMKAC 1 cut(s) 137
FokI GGATG 9 cut(s) 239, 1041, 1064, 1357, 1390, 1603, 2280, 2301, 2485
FriOI GRGCYC 4 cut(s) 54, 156, 539, 582
FspBI CTAG 3 cut(s) 615, 1200, 1743
FspI TGCGCA 1 cut(s) 1278
GlaI GCGC 2 cut(s) 608, 1278
GsaI CCCAGC 1 cut(s) 61
GsuI CTGGAG 3 cut(s) 1149, 1545, 2571
HapII CCGG 6 cut(s) 1251, 1257, 2211, 2243, 2277, 2497
HgaI GACGC 2 cut(s) 1052, 1789
HhaI GCGC 2 cut(s) 609, 1279
Hin6I GCGC 2 cut(s) 607, 1277
HinP1I GCGC 2 cut(s) 607, 1277
HincII GTYRAC 1 cut(s) 138
HindII GTYRAC 1 cut(s) 138
HindIII AAGCTT 1 cut(s) 2481
HpaII CCGG 6 cut(s) 1251, 1257, 2211, 2243, 2277, 2497
HphI GGTGA 2 cut(s) 404, 966
Hpy166II GTNNAC 2 cut(s) 138, 407
Hpy188III TCNNGA 6 cut(s) 97, 955, 992, 1458, 1536, 1832
Hpy8I GTNNAC 2 cut(s) 138, 407
Hpy99I CGWCG 1 cut(s) 71
HpyAV CCTTC 5 cut(s) 26, 296, 918, 951, 1409
HpyCH4III ACNGT 6 cut(s) 67, 148, 337, 871, 892, 2457
HpyCH4IV ACGT 1 cut(s) 167
HpyF3I CTNAG 9 cut(s) 507, 576, 581, 673, 1776, 1830, 1959, 2298, 2340
HpySE526I ACGT 1 cut(s) 167
HspAI GCGC 2 cut(s) 607, 1277
KpnI GGTACC 2 cut(s) 39, 1314
Kzo9I GATC 5 cut(s) 18, 90, 182, 625, 1944
LmnI GCTCC 3 cut(s) 457, 769, 1700
MaeI CTAG 3 cut(s) 615, 1200, 1743
MaeII ACGT 1 cut(s) 167
MaeIII GTNAC 8 cut(s) 163, 778, 1048, 1347, 1380, 1596, 1754, 2529
MalI GATC 5 cut(s) 20, 92, 184, 627, 1946
MbiI CCGCTC 1 cut(s) 620
MboI GATC 5 cut(s) 18, 90, 182, 625, 1944
MboII GAAGA 7 cut(s) 229, 963, 972, 1206, 1239, 1524, 1939
MfeI CAATTG 5 cut(s) 294, 633, 1916, 1949, 2420
MflI RGATCY 2 cut(s) 182, 625
MhlI GDGCHC 6 cut(s) 54, 156, 539, 582, 1513, 1660
MlsI TGGCCA 3 cut(s) 1001, 1175, 1798
MluI ACGCGT 1 cut(s) 1102
MluNI TGGCCA 3 cut(s) 1001, 1175, 1798
MlyI GAGTC 4 cut(s) 751, 960, 1140, 2470
MmeI TCCRAC 3 cut(s) 818, 829, 1840
Mox20I TGGCCA 3 cut(s) 1001, 1175, 1798
Mph1103I ATGCAT 1 cut(s) 1182
MroXI GAANNNNTTC 1 cut(s) 236
MscI TGGCCA 3 cut(s) 1001, 1175, 1798
MseI TTAA 6 cut(s) 668, 704, 1124, 1293, 2037, 2569
MslI CAYNNNNRTG 1 cut(s) 428
Msp20I TGGCCA 3 cut(s) 1001, 1175, 1798
MspA1I CMGCKG 1 cut(s) 16
MspI CCGG 6 cut(s) 1251, 1257, 2211, 2243, 2277, 2497
MssI GTTTAAAC 1 cut(s) 2038
MunI CAATTG 5 cut(s) 294, 633, 1916, 1949, 2420
Mva1269I GAATGC 1 cut(s) 2013
MvaI CCWGG 9 cut(s) 161, 200, 714, 1287, 1434, 1563, 2019, 2151, 2550
MvnI CGCG 2 cut(s) 1063, 1104
NciI CCSGG 4 cut(s) 1252, 2211, 2244, 2277
NcoI CCATGG 2 cut(s) 45, 900
NdeII GATC 5 cut(s) 18, 90, 182, 625, 1944
NlaIV GGNNCC 8 cut(s) 37, 51, 52, 906, 1255, 1312, 1461, 1528
NmuCI GTSAC 6 cut(s) 163, 1048, 1347, 1380, 1596, 2529
NsbI TGCGCA 1 cut(s) 1278
NsiI ATGCAT 1 cut(s) 1182
NspI RCATGY 3 cut(s) 1184, 2143, 2203
OliI CACNNNNGTG 1 cut(s) 428
PcsI WCGNNNNNNNCGW 1 cut(s) 1787
PctI GAATGC 1 cut(s) 2013
PdmI GAANNNNTTC 1 cut(s) 236
PfeI GAWTC 7 cut(s) 232, 829, 1552, 1894, 2302, 2323, 2344
PfoI TCCNGGA 2 cut(s) 712, 1561
PleI GAGTC 4 cut(s) 750, 959, 1140, 2470
PmeI GTTTAAAC 1 cut(s) 2038
PpsI GAGTC 4 cut(s) 750, 959, 1140, 2470
PpuMI RGGWCCY 1 cut(s) 905
Psp124BI GAGCTC 3 cut(s) 156, 539, 582
Psp5II RGGWCCY 1 cut(s) 905
Psp6I CCWGG 9 cut(s) 159, 198, 712, 1285, 1432, 1561, 2017, 2149, 2548
PspFI CCCAGC 1 cut(s) 57
PspGI CCWGG 9 cut(s) 159, 198, 712, 1285, 1432, 1561, 2017, 2149, 2548
PspN4I GGNNCC 8 cut(s) 37, 51, 52, 906, 1255, 1312, 1461, 1528
PspOMI GGGCCC 1 cut(s) 50
PspPPI RGGWCCY 1 cut(s) 905
PstNI CAGNNNCTG 4 cut(s) 133, 782, 1775, 2404
PsuI RGATCY 2 cut(s) 182, 625
PvuII CAGCTG 1 cut(s) 16
RsaI GTAC 6 cut(s) 37, 204, 1312, 1546, 2241, 2274
RsaNI GTAC 6 cut(s) 36, 203, 1311, 1545, 2240, 2273
RseI CAYNNNNRTG 1 cut(s) 428
SacI GAGCTC 3 cut(s) 156, 539, 582
SalI GTCGAC 1 cut(s) 136
SaqAI TTAA 6 cut(s) 668, 704, 1124, 1293, 2037, 2569
Sau3AI GATC 5 cut(s) 18, 90, 182, 625, 1944
SchI GAGTC 4 cut(s) 751, 960, 1140, 2470
SduI GDGCHC 6 cut(s) 54, 156, 539, 582, 1513, 1660
SfcI CTRYAG 3 cut(s) 333, 1035, 1068
SinI GGWCC 5 cut(s) 818, 905, 1254, 1460, 1834
SmiMI CAYNNNNRTG 1 cut(s) 428
SmlI CTYRAG 3 cut(s) 990, 1095, 2478
SmoI CTYRAG 3 cut(s) 990, 1095, 2478
SspI AATATT 1 cut(s) 472
SspMI CTAG 3 cut(s) 615, 1200, 1743
SstI GAGCTC 3 cut(s) 156, 539, 582
StyI CCWWGG 7 cut(s) 45, 900, 946, 1339, 1372, 1444, 1513
TaaI ACNGT 6 cut(s) 67, 148, 337, 871, 892, 2457
TaiI ACGT 1 cut(s) 170
TaqI TCGA 1 cut(s) 137
TauI GCSGC 5 cut(s) 44, 623, 790, 1790, 2410
TfiI GAWTC 7 cut(s) 232, 829, 1552, 1894, 2302, 2323, 2344
Tru1I TTAA 6 cut(s) 668, 704, 1124, 1293, 2037, 2569
Tru9I TTAA 6 cut(s) 668, 704, 1124, 1293, 2037, 2569
TscAI CASTG 3 cut(s) 435, 895, 1947
TseFI GTSAC 6 cut(s) 163, 1048, 1347, 1380, 1596, 2529
Tsp45I GTSAC 6 cut(s) 163, 1048, 1347, 1380, 1596, 2529
TspDTI ATGAA 8 cut(s) 480, 842, 1124, 1233, 1287, 1505, 1856, 1907
TspGWI ACGGA 2 cut(s) 223, 923
TspRI CASTG 3 cut(s) 435, 895, 1947
VpaK11BI GGWCC 5 cut(s) 818, 905, 1254, 1460, 1834
XagI CCTNNNNNAGG 2 cut(s) 945, 1560
XapI RAATTY 5 cut(s) 444, 451, 679, 2112, 2172
XceI RCATGY 3 cut(s) 1184, 2143, 2203
XmiI GTMKAC 1 cut(s) 137
XmnI GAANNNNTTC 1 cut(s) 236
XspI CTAG 3 cut(s) 615, 1200, 1743
Zsp2I ATGCAT 1 cut(s) 1182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.