RLG00000006568

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0005569
Physical Location & Seq
Forward (+)
8568906 .. 8579856
10951 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006568

Sequence Viewer

Length: 2484 bp
ATGTACAGATACATTGCATTAGCAAAGCCTCCACAGCCGAAACGCAGCGTTTTGGTCTCGGTCCTGCAAGACTTTACCGTTTATAGAGACTCCCAGGTTGGTCTCTCGCAAAGCAATGCGTCCCTGCTCTACATTTTCACCACCAGAAGAAGAAGAGGAAGAAGAGCCCCAACCTGTACTATCTCACTACTAGTTGGTGGGAAATTCAAACGTCTCCGCCTCATACGGCGTCGTTTCGTCTTCCGATTCCGTCTCCGGCTCTCTCAGCTCTCTCGCATTTCCGCATTTCCGAGACGGTTGACGGTCTTCGCCAACCGCTCGATGACGGAGAAGCAGTTGATCGTGGTCGTTGAAGGCACGGCGGCCATCGGGCCTTACTGGTCAGCCGTCGTCTCTGATTACGTCGAGAAGATCATCAGGTCTTTCTCTGGAAACGAGTTAAGTGGTCAGAAGCCCTCCACTTCTGTTGTTGAGATGTCTTTGGTCACATTCAATACTCATGGATCTTATTCCGGTTGCTTGGTACAACGGAGTGGTTGGACAAGAGATGTTGATCGTTTCTTACAATGGCTGTCAGCTATTCCCTTAGCAGGTGGTGGTTTTAGTGATGCTGCAATTGCAGAAGGGCTTTCTGAAGCCCTAATGATGTTCACTACCGTTCAAAATGGAAGCCAAAATCAACAAAATGTGGATTTTCAAAAGCATTGCATTCTTGTTGCTGCTAGTAACCCTCATCCCCTGCCCACTCCAGTCTATAGACCACAAATTCAAAATGTGGAGAAGAGTGAAAATATTGATGCACAAACCGAAAGCCGTCTGTATGATGCTGAGGCTGTTGCTAAATCATTTGCACAGTGCCTTGTTTCTCTGTCAGTCATCTGTCCGAAACATCTTCCTAAACTCAGAGCCATTTACAATGCGGGAAAGCGCAACCCCCGGGCAGCAGATCCACCGATTGATAATGTGAAAAACCCCCACTTTCTCGTTCTAATCTCAGAGAATTTTATGGAGGCCCGGGCTACTTTAAGTCGTCCTGGAAGTACAACTTTGCCCTCAAATCCGAGTACTGTAAAGATGGACATAGCTCCTGTTGCATCAGTGACAGGGCCGCCTCCAACTTCTATGCCATCTGTGAATGGATCTGTTATGAATCGGCCATCAGTTTCTGTTAACAATGTGCCTCCTGCGACAGTGAAAGTGGAGCCAAGCACTGTAAGTTCAATGGTAGCTGGACCTCCTTTTGCACACGTTCCATCTGTTCGTCCTCCTTCCCAAGGAGTTCCAACCTTGCAAACTTCTTCACCATCTTCTGGTGAGATGATTACAAACAATGAGAGTGTGCCAGATTTGAAACCTATAGTGAGTAATGTGACACATCAACTACGTCCTGTGGGTAGCATCCTCAATAATATCTCTCAAGCACGCGTGATGAATTCTGCAGCCTTATCTGGAGGATCTTCTATAGGTCTCCAAAACATGGGTCAAAATCCTATGGCCATGCATGTATCAAACATGATATCGAGTGGAATGGCATCTTCGGTGGCTGCTGCTCAAAATGTATTTTCATCTTCAGGGCCTCACACTCTTGCACAGGTTGCTCAGAGCACAGGTCTTAGTTCATTTACTTCAGGTACTTCCAATGTGTCTGGAAATTCTAACCTTGGGATGTCGCAACCAATGAGTAACCTCCAAGGTGGTGTTAGCATTGGCCAATCTGTTCCTGGCATCAGTCAAGGAAATCTCTCAGGACCCCAGATGGTTCAAAGTGGCATTGGAATGACTCCAAACATGATGAGTGGCCTTGGTTCATCAGGTACCTCCTCTGGAACTGGGACAATGATTCCCACTCCAGGCATGTCTCAACAGGCACAATCTGGGATGCAGTCACTTGGTGCAAACAATAGTTCAGTTTCTAATGTGCCGTTGTCACAACAGACGTCAAGTGCTTTGCAGTCTGCACAATCCAAATATGTGAAAGTGTGGGAGGGAAACTTATCTGGGCAGAGACAAGGGCAACCTGTGTTTATTACTAGATTGGAGGGATACAGAAGTGCTACAGCATCTGAGACGCTTGCAGCAAACTGGCCACCAACAATGCAAATAGTCAGGCTCATATCTCAGGATCACATGAATAACAAGCAATATGTGGGAAGGGCAGACTTTCTAGTATTTCGGGCAATGAATCAGCATGGATTTCTTGGGCAGTTGCAAGAAAAGAAGCTTTGTGCTGTAATCCAATTGCCGTCTCAGACACTACTGCTCTCTGTTTCGGACAAAGCTTGCCGCTTGATTGGAATGCTTTTCCCAGGGTCACAACAGCAAATCCCACAACTGCAGCAACTGCAGCAACAACACCAACAGCAACAGTTGGTTGGGGGTGGAATGGGTCAAGCTTATGGTCAAGGCCGGTCACAATTACTTTCACAAGGACAAGTTTCATCACAACAAGGGCCTAATATGCCCGGAGGAGGCTTTATGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med25_VWA

Protein Analysis

828

Amino Acids

88.45

Weight (kDa)

10.2

Isoelectric Point (pI)

55.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med25_VWA PF11265 109 - 333 4.3e-44 Mediator complex subunit 25 von Willebrand factor type A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 581
AatII GACGTC 1 cut(s) 1940
Acc36I ACCTGC 1 cut(s) 581
Acc65I GGTACC 1 cut(s) 1814
AccB1I GGYRCC 1 cut(s) 1814
AccB7I CCANNNNNTGG 2 cut(s) 1310, 1477
AccBSI CCGCTC 1 cut(s) 318
AccII CGCG 1 cut(s) 1425
AciI CCGC 7 cut(s) 217, 282, 316, 362, 920, 1109, 2284
AclWI GGATC 5 cut(s) 511, 941, 1147, 1462, 2130
AcoI YGGCCR 5 cut(s) 363, 1154, 1494, 1708, 2084
AcsI RAATTY 5 cut(s) 203, 765, 1000, 1432, 1651
AcuI CTGAAG 3 cut(s) 654, 1554, 1611
AcyI GRCGYC 2 cut(s) 229, 1937
AdeI CACNNNGTG 1 cut(s) 1892
AfaI GTAC 7 cut(s) 5, 178, 525, 1042, 1066, 1633, 1816
AfiI CCNNNNNNNGG 6 cut(s) 590, 1274, 1310, 1477, 1850, 2468
AflIII ACRYGT 2 cut(s) 1246, 1423
AgsI TTSAA 9 cut(s) 208, 353, 493, 662, 698, 770, 1221, 1351, 1763
AhlI ACTAGT 1 cut(s) 190
AjnI CCWGG 5 cut(s) 93, 1033, 1720, 1849, 2305
AluBI AGCT 7 cut(s) 268, 578, 1085, 1229, 2221, 2279, 2393
AluI AGCT 7 cut(s) 268, 578, 1085, 1229, 2221, 2279, 2393
Alw21I GWGCWC 1 cut(s) 1607
AlwI GGATC 5 cut(s) 511, 941, 1147, 1462, 2130
AlwNI CAGNNNCTG 3 cut(s) 1166, 2063, 2341
Ama87I CYCGRG 2 cut(s) 936, 1014
ApoI RAATTY 5 cut(s) 203, 765, 1000, 1432, 1651
ArsI GACNNNNNNTTYG 2 cut(s) 1465, 1497
Asp718I GGTACC 1 cut(s) 1814
AspLEI GCGC 1 cut(s) 930
AspS9I GGNCC 8 cut(s) 61, 371, 1012, 1106, 1232, 1574, 1748, 2450
AsuC2I CCSGG 5 cut(s) 937, 938, 1015, 1016, 2463
AsuHPI GGTGA 3 cut(s) 130, 1293, 1325
AvaI CYCGRG 2 cut(s) 936, 1014
AvaII GGWCC 3 cut(s) 61, 1232, 1748
BaeI ACNNNNGTAYC 2 cut(s) 2035, 2068
BalI TGGCCA 3 cut(s) 1496, 1710, 2086
BanI GGYRCC 1 cut(s) 1814
BanII GRGCYC 1 cut(s) 169
BbsI GAAGAC 2 cut(s) 232, 298
Bbv12I GWGCWC 1 cut(s) 1607
BbvCI CCTCAGC 1 cut(s) 828
BccI CCATC 7 cut(s) 374, 1069, 1135, 1165, 1261, 1312, 1750
BceAI ACGGC 6 cut(s) 242, 371, 375, 798, 1906, 2227
BciT130I CCWGG 5 cut(s) 95, 1035, 1722, 1851, 2307
BciVI GTATCC 1 cut(s) 2036
BcnI CCSGG 5 cut(s) 937, 938, 1015, 1016, 2463
BcuI ACTAGT 1 cut(s) 190
BfaI CTAG 4 cut(s) 191, 723, 2031, 2165
BfmI CTRYAG 7 cut(s) 754, 1356, 1437, 1461, 2055, 2333, 2342
BfuAI ACCTGC 1 cut(s) 581
BfuI GTATCC 1 cut(s) 2036
BmcAI AGTACT 1 cut(s) 1066
Bme18I GGWCC 3 cut(s) 61, 1232, 1748
BmeT110I CYCGRG 2 cut(s) 936, 1014
BmgT120I GGNCC 8 cut(s) 61, 371, 1012, 1106, 1232, 1574, 1748, 2450
BmiI GGNNCC 3 cut(s) 1203, 1750, 1816
BmrI ACTGGG 1 cut(s) 1839
BmsI GCATC 9 cut(s) 598, 787, 814, 1103, 1407, 1541, 1734, 1869, 2069
BmuI ACTGGG 1 cut(s) 1839
BpiI GAAGAC 2 cut(s) 232, 298
BpmI CTGGAG 3 cut(s) 732, 1470, 1833
Bpu10I CCTNAGC 2 cut(s) 586, 828
BpuEI CTTGAG 1 cut(s) 1401
BpuMI CCSGG 5 cut(s) 937, 938, 1015, 1016, 2463
BsaBI GATNNNNATC 1 cut(s) 552
BsaHI GRCGYC 2 cut(s) 229, 1937
BsaI GGTCTC 3 cut(s) 61, 107, 1472
BsaWI WCCGGW 1 cut(s) 512
BsaXI ACNNNNNCTCC 2 cut(s) 320, 350
Bsc4I CCNNNNNNNGG 6 cut(s) 590, 1274, 1310, 1477, 1850, 2468
Bse118I RCCGGY 1 cut(s) 2406
Bse1I ACTGG 4 cut(s) 383, 749, 1834, 2087
Bse3DI GCAATG 4 cut(s) 12, 121, 703, 2184
Bse8I GATNNNNATC 1 cut(s) 552
BseBI CCWGG 5 cut(s) 95, 1035, 1722, 1851, 2307
BseGI GGATG 4 cut(s) 733, 1398, 1671, 1884
BseJI GATNNNNATC 1 cut(s) 552
BseLI CCNNNNNNNGG 6 cut(s) 590, 1274, 1310, 1477, 1850, 2468
BseMI GCAATG 4 cut(s) 12, 121, 703, 2184
BseMII CTCAG 9 cut(s) 278, 819, 916, 1008, 1613, 1758, 2055, 2132, 2261
BseNI ACTGG 4 cut(s) 383, 749, 1834, 2087
BseRI GAGGAG 2 cut(s) 1810, 2481
BsgI GTGCAG 1 cut(s) 1941
Bsh1236I CGCG 1 cut(s) 1425
BshNI GGYRCC 1 cut(s) 1814
BsiHKAI GWGCWC 1 cut(s) 1607
BsiHKCI CYCGRG 2 cut(s) 936, 1014
BsiSI CCGG 6 cut(s) 256, 513, 937, 1015, 2407, 2463
BslFI GGGAC 2 cut(s) 106, 1846
BslI CCNNNNNNNGG 6 cut(s) 590, 1274, 1310, 1477, 1850, 2468
BsmBI CGTCTC 6 cut(s) 218, 257, 286, 397, 2060, 2250
BsmFI GGGAC 2 cut(s) 106, 1846
BsmI GAATGC 2 cut(s) 708, 2301
Bso31I GGTCTC 3 cut(s) 61, 107, 1472
BsoBI CYCGRG 2 cut(s) 936, 1014
Bsp1286I GDGCHC 2 cut(s) 169, 1607
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 8 cut(s) 339, 411, 503, 553, 946, 1139, 1454, 2122
BspACI CCGC 7 cut(s) 217, 282, 316, 362, 920, 1109, 2284
BspCNI CTCAG 9 cut(s) 277, 820, 915, 1007, 1612, 1757, 2056, 2131, 2260
BspFNI CGCG 1 cut(s) 1425
BspLI GGNNCC 3 cut(s) 1203, 1750, 1816
BspMAI CTGCAG 3 cut(s) 1441, 2337, 2346
BspMI ACCTGC 1 cut(s) 581
BspPI GGATC 5 cut(s) 511, 941, 1147, 1462, 2130
BspQI GCTCTTC 1 cut(s) 157
BspT107I GGYRCC 1 cut(s) 1814
BspTNI GGTCTC 3 cut(s) 61, 107, 1472
BsrBI CCGCTC 1 cut(s) 318
BsrDI GCAATG 4 cut(s) 12, 121, 703, 2184
BsrFI RCCGGY 1 cut(s) 2406
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 4 cut(s) 383, 749, 1834, 2087
BssAI RCCGGY 1 cut(s) 2406
BssMI GATC 8 cut(s) 339, 411, 503, 553, 946, 1139, 1454, 2122
BssNI GRCGYC 2 cut(s) 229, 1937
BssT1I CCWWGG 4 cut(s) 1273, 1660, 1690, 1801
Bst2UI CCWGG 5 cut(s) 95, 1035, 1722, 1851, 2307
Bst4CI ACNGT 9 cut(s) 79, 297, 304, 658, 855, 1069, 1192, 1213, 2367
Bst6I CTCTTC 3 cut(s) 148, 157, 776
BstACI GRCGYC 2 cut(s) 229, 1937
BstAPI GCANNNNNTGC 2 cut(s) 1595, 2341
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 3 cut(s) 1423, 2073, 2281
BstENI CCTNNNNNAGG 1 cut(s) 1272
BstF5I GGATG 4 cut(s) 733, 1398, 1671, 1884
BstFNI CGCG 1 cut(s) 1425
BstHHI GCGC 1 cut(s) 930
BstKTI GATC 8 cut(s) 342, 414, 506, 556, 949, 1142, 1457, 2125
BstMBI GATC 8 cut(s) 339, 411, 503, 553, 946, 1139, 1454, 2122
BstMWI GCNNNNNNNGC 8 cut(s) 34, 265, 617, 1091, 1595, 2341, 2344, 2458
BstNI CCWGG 5 cut(s) 95, 1035, 1722, 1851, 2307
BstNSI RCATGY 2 cut(s) 1505, 1858
BstSFI CTRYAG 7 cut(s) 754, 1356, 1437, 1461, 2055, 2333, 2342
BstUI CGCG 1 cut(s) 1425
BstV2I GAAGAC 2 cut(s) 232, 298
BstX2I RGATCY 4 cut(s) 503, 946, 1139, 1454
BstYI RGATCY 4 cut(s) 503, 946, 1139, 1454
BsuI GTATCC 1 cut(s) 2036
BtsCI GGATG 4 cut(s) 733, 1398, 1671, 1884
BtsIMutI CAGTG 4 cut(s) 860, 1104, 1197, 1209
BveI ACCTGC 1 cut(s) 581
Cac8I GCNNGC 3 cut(s) 1423, 2073, 2281
CaiI CAGNNNCTG 3 cut(s) 1166, 2063, 2341
CfoI GCGC 1 cut(s) 930
Cfr10I RCCGGY 1 cut(s) 2406
Cfr13I GGNCC 8 cut(s) 61, 371, 1012, 1106, 1232, 1574, 1748, 2450
Cfr9I CCCGGG 2 cut(s) 936, 1014
CseI GACGC 3 cut(s) 108, 218, 2077
Csp6I GTAC 7 cut(s) 4, 177, 524, 1041, 1065, 1632, 1815
CspCI CAANNNNNGTGG 2 cut(s) 448, 483
CviAII CATG 9 cut(s) 500, 1477, 1498, 1502, 1513, 1789, 1855, 2128, 2189
CviQI GTAC 7 cut(s) 4, 177, 524, 1041, 1065, 1632, 1815
DpnI GATC 8 cut(s) 341, 413, 505, 555, 948, 1141, 1456, 2124
DpnII GATC 8 cut(s) 339, 411, 503, 553, 946, 1139, 1454, 2122
DraIII CACNNNGTG 1 cut(s) 1892
EaeI YGGCCR 5 cut(s) 363, 1154, 1494, 1708, 2084
Eam1104I CTCTTC 3 cut(s) 148, 157, 776
EarI CTCTTC 3 cut(s) 148, 157, 776
EciI GGCGGA 1 cut(s) 206
Eco130I CCWWGG 4 cut(s) 1273, 1660, 1690, 1801
Eco24I GRGCYC 1 cut(s) 169
Eco31I GGTCTC 3 cut(s) 61, 107, 1472
Eco32I GATATC 1 cut(s) 1518
Eco47I GGWCC 3 cut(s) 61, 1232, 1748
Eco57I CTGAAG 3 cut(s) 654, 1554, 1611
Eco88I CYCGRG 2 cut(s) 936, 1014
EcoNI CCTNNNNNAGG 1 cut(s) 1272
EcoO109I RGGNCCY 3 cut(s) 1574, 1748, 2450
EcoRI GAATTC 1 cut(s) 1432
EcoRII CCWGG 5 cut(s) 93, 1033, 1720, 1849, 2305
EcoRV GATATC 1 cut(s) 1518
EcoT14I CCWWGG 4 cut(s) 1273, 1660, 1690, 1801
EcoT22I ATGCAT 1 cut(s) 1503
EcoT38I GRGCYC 1 cut(s) 169
ErhI CCWWGG 4 cut(s) 1273, 1660, 1690, 1801
Esp3I CGTCTC 6 cut(s) 218, 257, 286, 397, 2060, 2250
FaeI CATG 9 cut(s) 503, 1480, 1501, 1505, 1516, 1792, 1858, 2131, 2192
FalI AAGNNNNNCTT 2 cut(s) 1030, 1062
FaqI GGGAC 2 cut(s) 106, 1846
FatI CATG 9 cut(s) 499, 1476, 1497, 1501, 1512, 1788, 1854, 2127, 2188
FauI CCCGC 1 cut(s) 913
FokI GGATG 4 cut(s) 720, 1385, 1678, 1891
FriOI GRGCYC 1 cut(s) 169
FspBI CTAG 4 cut(s) 191, 723, 2031, 2165
GlaI GCGC 1 cut(s) 929
GsuI CTGGAG 3 cut(s) 732, 1470, 1833
HapII CCGG 6 cut(s) 256, 513, 937, 1015, 2407, 2463
HgaI GACGC 3 cut(s) 108, 218, 2077
HhaI GCGC 1 cut(s) 930
Hin1I GRCGYC 2 cut(s) 229, 1937
Hin1II CATG 9 cut(s) 503, 1480, 1501, 1505, 1516, 1792, 1858, 2131, 2192
Hin6I GCGC 1 cut(s) 928
HinP1I GCGC 1 cut(s) 928
HincII GTYRAC 2 cut(s) 300, 1171
HindII GTYRAC 2 cut(s) 300, 1171
HindIII AAGCTT 3 cut(s) 2219, 2277, 2391
HinfI GANTC 6 cut(s) 89, 246, 1150, 1780, 1840, 2182
HpaI GTTAAC 1 cut(s) 1171
HpaII CCGG 6 cut(s) 256, 513, 937, 1015, 2407, 2463
HphI GGTGA 3 cut(s) 130, 1293, 1325
Hpy166II GTNNAC 3 cut(s) 300, 651, 1171
Hpy188III TCNNGA 7 cut(s) 406, 429, 1449, 1647, 1746, 1824, 2120
Hpy8I GTNNAC 3 cut(s) 300, 651, 1171
Hpy99I CGWCG 3 cut(s) 234, 392, 407
HpyAV CCTTC 4 cut(s) 347, 617, 1278, 2145
HpyCH4III ACNGT 9 cut(s) 79, 297, 304, 658, 855, 1069, 1192, 1213, 2367
HpyCH4IV ACGT 5 cut(s) 211, 402, 1248, 1384, 1937
HpyF10VI GCNNNNNNNGC 8 cut(s) 34, 265, 617, 1091, 1595, 2341, 2344, 2458
HpySE526I ACGT 5 cut(s) 211, 402, 1248, 1384, 1937
Hsp92I GRCGYC 2 cut(s) 229, 1937
Hsp92II CATG 9 cut(s) 503, 1480, 1501, 1505, 1516, 1792, 1858, 2131, 2192
HspAI GCGC 1 cut(s) 928
KpnI GGTACC 1 cut(s) 1818
KspAI GTTAAC 1 cut(s) 1171
Kzo9I GATC 8 cut(s) 339, 411, 503, 553, 946, 1139, 1454, 2122
LguI GCTCTTC 1 cut(s) 157
LmnI GCTCC 2 cut(s) 1090, 1201
LweI GCATC 9 cut(s) 598, 787, 814, 1103, 1407, 1541, 1734, 1869, 2069
MaeI CTAG 4 cut(s) 191, 723, 2031, 2165
MaeII ACGT 5 cut(s) 211, 402, 1248, 1384, 1937
MaeIII GTNAC 9 cut(s) 484, 725, 1099, 1369, 1682, 1884, 1926, 2310, 2409
MalI GATC 8 cut(s) 341, 413, 505, 555, 948, 1141, 1456, 2124
MbiI CCGCTC 1 cut(s) 318
MboI GATC 8 cut(s) 339, 411, 503, 553, 946, 1139, 1454, 2122
MfeI CAATTG 2 cut(s) 615, 2237
MflI RGATCY 4 cut(s) 503, 946, 1139, 1454
MhlI GDGCHC 2 cut(s) 169, 1607
MlsI TGGCCA 3 cut(s) 1496, 1710, 2086
MluCI AATT 8 cut(s) 203, 615, 765, 1000, 1432, 1651, 2237, 2414
MluI ACGCGT 1 cut(s) 1423
MluNI TGGCCA 3 cut(s) 1496, 1710, 2086
MlyI GAGTC 2 cut(s) 83, 1774
MmeI TCCRAC 3 cut(s) 518, 1139, 1307
Mox20I TGGCCA 3 cut(s) 1496, 1710, 2086
Mph1103I ATGCAT 1 cut(s) 1503
MscI TGGCCA 3 cut(s) 1496, 1710, 2086
MseI TTAA 4 cut(s) 440, 1025, 1170, 2482
MslI CAYNNNNRTG 1 cut(s) 1775
Msp20I TGGCCA 3 cut(s) 1496, 1710, 2086
MspI CCGG 6 cut(s) 256, 513, 937, 1015, 2407, 2463
MunI CAATTG 2 cut(s) 615, 2237
Mva1269I GAATGC 2 cut(s) 708, 2301
MvaI CCWGG 5 cut(s) 95, 1035, 1722, 1851, 2307
MvnI CGCG 1 cut(s) 1425
MwoI GCNNNNNNNGC 8 cut(s) 34, 265, 617, 1091, 1595, 2341, 2344, 2458
NciI CCSGG 5 cut(s) 937, 938, 1015, 1016, 2463
NdeII GATC 8 cut(s) 339, 411, 503, 553, 946, 1139, 1454, 2122
NlaIII CATG 9 cut(s) 503, 1480, 1501, 1505, 1516, 1792, 1858, 2131, 2192
NlaIV GGNNCC 3 cut(s) 1203, 1750, 1816
NmuCI GTSAC 7 cut(s) 484, 1099, 1369, 1884, 1926, 2310, 2409
NsiI ATGCAT 1 cut(s) 1503
NspI RCATGY 2 cut(s) 1505, 1858
PaqCI CACCTGC 1 cut(s) 581
PasI CCCWGGG 1 cut(s) 2306
PciSI GCTCTTC 1 cut(s) 157
PctI GAATGC 2 cut(s) 708, 2301
PfeI GAWTC 4 cut(s) 246, 1150, 1840, 2182
PflMI CCANNNNNTGG 2 cut(s) 1310, 1477
PfoI TCCNGGA 1 cut(s) 1033
PleI GAGTC 2 cut(s) 83, 1774
PpsI GAGTC 2 cut(s) 83, 1774
PpuMI RGGWCCY 1 cut(s) 1748
Psp5II RGGWCCY 1 cut(s) 1748
Psp6I CCWGG 5 cut(s) 93, 1033, 1720, 1849, 2305
PspGI CCWGG 5 cut(s) 93, 1033, 1720, 1849, 2305
PspN4I GGNNCC 3 cut(s) 1203, 1750, 1816
PspPI GGNCC 8 cut(s) 61, 371, 1012, 1106, 1232, 1574, 1748, 2450
PspPPI RGGWCCY 1 cut(s) 1748
PstI CTGCAG 3 cut(s) 1441, 2337, 2346
PstNI CAGNNNCTG 3 cut(s) 1166, 2063, 2341
PsuI RGATCY 4 cut(s) 503, 946, 1139, 1454
RsaI GTAC 7 cut(s) 5, 178, 525, 1042, 1066, 1633, 1816
RsaNI GTAC 7 cut(s) 4, 177, 524, 1041, 1065, 1632, 1815
RseI CAYNNNNRTG 1 cut(s) 1775
SapI GCTCTTC 1 cut(s) 157
SaqAI TTAA 4 cut(s) 440, 1025, 1170, 2482
Sau3AI GATC 8 cut(s) 339, 411, 503, 553, 946, 1139, 1454, 2122
Sau96I GGNCC 8 cut(s) 61, 371, 1012, 1106, 1232, 1574, 1748, 2450
ScaI AGTACT 1 cut(s) 1066
SchI GAGTC 2 cut(s) 83, 1774
SduI GDGCHC 2 cut(s) 169, 1607
SfaNI GCATC 9 cut(s) 598, 787, 814, 1103, 1407, 1541, 1734, 1869, 2069
SfcI CTRYAG 7 cut(s) 754, 1356, 1437, 1461, 2055, 2333, 2342
SinI GGWCC 3 cut(s) 61, 1232, 1748
SmaI CCCGGG 2 cut(s) 938, 1016
SmiMI CAYNNNNRTG 1 cut(s) 1775
SmlI CTYRAG 1 cut(s) 1416
SmoI CTYRAG 1 cut(s) 1416
SpeI ACTAGT 1 cut(s) 190
SrfI GCCCGGGC 1 cut(s) 1016
Sse9I AATT 8 cut(s) 203, 615, 765, 1000, 1432, 1651, 2237, 2414
SsiI CCGC 7 cut(s) 217, 282, 316, 362, 920, 1109, 2284
SspI AATATT 1 cut(s) 793
SspMI CTAG 4 cut(s) 191, 723, 2031, 2165
StyI CCWWGG 4 cut(s) 1273, 1660, 1690, 1801
TaaI ACNGT 9 cut(s) 79, 297, 304, 658, 855, 1069, 1192, 1213, 2367
TaiI ACGT 5 cut(s) 214, 405, 1251, 1387, 1940
TaqI TCGA 3 cut(s) 320, 405, 1520
TaqII GACCGA 1 cut(s) 49
TasI AATT 8 cut(s) 203, 615, 765, 1000, 1432, 1651, 2237, 2414
TatI WGTACW 4 cut(s) 3, 176, 1040, 1064
TauI GCSGC 3 cut(s) 365, 1111, 2286
TfiI GAWTC 4 cut(s) 246, 1150, 1840, 2182
Tru1I TTAA 4 cut(s) 440, 1025, 1170, 2482
Tru9I TTAA 4 cut(s) 440, 1025, 1170, 2482
TscAI CASTG 4 cut(s) 860, 1104, 1197, 1216
TseFI GTSAC 7 cut(s) 484, 1099, 1369, 1884, 1926, 2310, 2409
Tsp45I GTSAC 7 cut(s) 484, 1099, 1369, 1884, 1926, 2310, 2409
TspDTI ATGAA 8 cut(s) 1163, 1445, 1554, 1608, 1797, 2144, 2195, 2427
TspGWI ACGGA 3 cut(s) 239, 341, 544
TspMI CCCGGG 2 cut(s) 936, 1014
TspRI CASTG 4 cut(s) 860, 1104, 1197, 1216
Van91I CCANNNNNTGG 2 cut(s) 1310, 1477
VpaK11BI GGWCC 3 cut(s) 61, 1232, 1748
XagI CCTNNNNNAGG 1 cut(s) 1272
XapI RAATTY 5 cut(s) 203, 765, 1000, 1432, 1651
XceI RCATGY 2 cut(s) 1505, 1858
XcmI CCANNNNNNNNNTGG 1 cut(s) 1718
XmaI CCCGGG 2 cut(s) 936, 1014
XspI CTAG 4 cut(s) 191, 723, 2031, 2165
ZraI GACGTC 1 cut(s) 1938
ZrmI AGTACT 1 cut(s) 1066
Zsp2I ATGCAT 1 cut(s) 1503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.