AT1G27700

Syntaxin 6, N-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010278
Physical Location & Seq
Reverse (-)
9639859 .. 9641902
2044 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G27700.1

Sequence Viewer

Length: 894 bp
ATGACGACGAGTTTGGATCGGTGGGAGAAAGATCCTTTCTTTCCCGCTGCTGAAGAAGTTCAGGAATCTGCCGACAGGATGGAGTCAGCGTACAGGACATGGCTCAATGGGAAACGAGACTCTTCAAAAGTATGGGACTCTGAGCAGCTTCATAGAGATCTTCACGCAGCTCTTGGAACCACCAAATGGCAGTTAGATGAATTCCAAAAAGCTGTCAAATCTAGCTATGACAATCGTTTGAGTGATGAAACTAGAGATAGGCATCGTGAGTTTACTTTCGTAATGGAAGCTCAAGTGTCGAAAATCGAGAAATCTCTCAAGGAAGCTGCGCAATCCGATGGGAAAGGAACACCGAGATGGGTTCGTTTGGATGAAGATGATCGTAATGAGCTTGCTCTCTTCTTGACTGGTCCATCTGAATCTGCTAAAGCAAATCCTAATGGACATAGAAGGACTGCAAGTGCTGCTGAATTCAGTGCTTGGAACATTGCGGTTTCTGATGATGGTTTAGTGAAGAAATCTTGTGATGAGCCAGTGGTTCGACCTCCGAGGAAAGTACCTAGCTTCTCTGGATTCTTGAATTATATGGATCCTGTGTCCAAGCATTGCAATAGAAAGTGGAAGGGTTTAGATCGTCAAGGAGACTCTGATGCTGCTTTATTACCTATCCAAGCTAACCAACAAGATATGAATGGATGTTCAGAGAGGGGCAAGAGTTGTATGGAGTGTGAGGAGGACTGTTATGAAAAGCAGCTTCATGGTTGGTATGGAGCTCTACAGAGGCAACTCCAACGGTCTCAGTATCGAATGCGATATAGTAAATCGGTTCACGCTGCAATTTGGATTATTCTTTTAGTTTTTCTTATTGTGGTAGTTGCAGTGCATTCAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Syntaxin-6_N

Protein Analysis

297

Amino Acids

34.05

Weight (kDa)

6.47

Isoelectric Point (pI)

47.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
STX6_10_61_N PF09177 11 - 103 2.6e-20 Syntaxin 6/10/61, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 330
AccB7I CCANNNNNTGG 1 cut(s) 186
AciI CCGC 2 cut(s) 45, 491
AclWI GGATC 4 cut(s) 24, 26, 584, 597
AcsI RAATTY 2 cut(s) 200, 470
AcuI CTGAAG 1 cut(s) 72
AfaI GTAC 2 cut(s) 92, 558
AfiI CCNNNNNNNGG 1 cut(s) 186
AgsI TTSAA 3 cut(s) 126, 580, 888
Alw21I GWGCWC 1 cut(s) 775
Alw26I GTCTC 3 cut(s) 111, 636, 801
AlwI GGATC 4 cut(s) 24, 26, 584, 597
ApeKI GCWGC 8 cut(s) 47, 145, 167, 326, 464, 653, 751, 833
ApoI RAATTY 2 cut(s) 200, 470
ArsI GACNNNNNNTTYG 1 cut(s) 27
Asp700I GAANNNNTTC 1 cut(s) 57
AspLEI GCGC 1 cut(s) 331
AspS9I GGNCC 1 cut(s) 410
AvaII GGWCC 1 cut(s) 410
BamHI GGATCC 1 cut(s) 589
BanII GRGCYC 1 cut(s) 775
Bbv12I GWGCWC 1 cut(s) 775
BbvI GCAGC 8 cut(s) 34, 157, 179, 313, 451, 640, 763, 820
BccI CCATC 5 cut(s) 73, 332, 351, 421, 497
BcoDI GTCTC 3 cut(s) 111, 636, 801
BfaI CTAG 3 cut(s) 222, 252, 561
BfmI CTRYAG 1 cut(s) 776
BglII AGATCT 1 cut(s) 157
BisI GCNGC 8 cut(s) 48, 146, 168, 327, 465, 654, 752, 834
BlsI GCNGC 8 cut(s) 49, 147, 169, 328, 466, 655, 753, 835
Bme18I GGWCC 1 cut(s) 410
BmgT120I GGNCC 1 cut(s) 410
BmiI GGNNCC 2 cut(s) 178, 591
BmsI GCATC 2 cut(s) 271, 640
BpuEI CTTGAG 2 cut(s) 276, 302
BsaBI GATNNNNATC 1 cut(s) 261
BsaI GGTCTC 1 cut(s) 801
BsaJI CCNNGG 1 cut(s) 548
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bsc4I CCNNNNNNNGG 1 cut(s) 186
Bse1I ACTGG 2 cut(s) 412, 533
Bse3DI GCAATG 2 cut(s) 486, 604
Bse8I GATNNNNATC 1 cut(s) 261
BseDI CCNNGG 1 cut(s) 548
BseGI GGATG 3 cut(s) 84, 376, 701
BseJI GATNNNNATC 1 cut(s) 261
BseLI CCNNNNNNNGG 1 cut(s) 186
BseMI GCAATG 2 cut(s) 486, 604
BseMII CTCAG 2 cut(s) 132, 812
BseNI ACTGG 2 cut(s) 412, 533
BseRI GAGGAG 1 cut(s) 746
BseXI GCAGC 8 cut(s) 34, 157, 179, 313, 451, 640, 763, 820
BsiHKAI GWGCWC 1 cut(s) 775
BslFI GGGAC 1 cut(s) 149
BslI CCNNNNNNNGG 1 cut(s) 186
BsmAI GTCTC 3 cut(s) 111, 636, 801
BsmFI GGGAC 1 cut(s) 149
BsmI GAATGC 2 cut(s) 813, 883
Bso31I GGTCTC 1 cut(s) 801
Bsp1286I GDGCHC 1 cut(s) 775
Bsp143I GATC 6 cut(s) 16, 31, 157, 379, 589, 631
BspACI CCGC 2 cut(s) 45, 491
BspCNI CTCAG 2 cut(s) 133, 811
BspLI GGNNCC 2 cut(s) 178, 591
BspPI GGATC 4 cut(s) 24, 26, 584, 597
BspTNI GGTCTC 1 cut(s) 801
BsrDI GCAATG 2 cut(s) 486, 604
BsrI ACTGG 2 cut(s) 412, 533
BssECI CCNNGG 1 cut(s) 548
BssMI GATC 6 cut(s) 16, 31, 157, 379, 589, 631
Bst4CI ACNGT 2 cut(s) 740, 795
Bst6I CTCTTC 2 cut(s) 127, 404
BstAPI GCANNNNNTGC 1 cut(s) 464
BstC8I GCNNGC 1 cut(s) 393
BstDEI CTNAG 2 cut(s) 141, 798
BstF5I GGATG 3 cut(s) 84, 376, 701
BstHHI GCGC 1 cut(s) 331
BstKTI GATC 6 cut(s) 19, 34, 160, 382, 592, 634
BstMAI GTCTC 3 cut(s) 111, 636, 801
BstMBI GATC 6 cut(s) 16, 31, 157, 379, 589, 631
BstMWI GCNNNNNNNGC 1 cut(s) 464
BstSFI CTRYAG 1 cut(s) 776
BstV1I GCAGC 8 cut(s) 34, 157, 179, 313, 451, 640, 763, 820
BstX2I RGATCY 3 cut(s) 31, 157, 589
BstYI RGATCY 3 cut(s) 31, 157, 589
BtsCI GGATG 3 cut(s) 84, 376, 701
BtsI GCAGTG 1 cut(s) 885
BtsIMutI CAGTG 3 cut(s) 481, 540, 885
Cac8I GCNNGC 1 cut(s) 393
CfoI GCGC 1 cut(s) 331
Cfr13I GGNCC 1 cut(s) 410
Csp6I GTAC 2 cut(s) 91, 557
CviAII CATG 2 cut(s) 99, 758
CviQI GTAC 2 cut(s) 91, 557
DdeI CTNAG 2 cut(s) 141, 798
DpnI GATC 6 cut(s) 18, 33, 159, 381, 591, 633
DpnII GATC 6 cut(s) 16, 31, 157, 379, 589, 631
Eam1104I CTCTTC 2 cut(s) 127, 404
EarI CTCTTC 2 cut(s) 127, 404
Ecl136II GAGCTC 1 cut(s) 773
Eco24I GRGCYC 1 cut(s) 775
Eco31I GGTCTC 1 cut(s) 801
Eco47I GGWCC 1 cut(s) 410
Eco53kI GAGCTC 1 cut(s) 773
Eco57I CTGAAG 1 cut(s) 72
EcoICRI GAGCTC 1 cut(s) 773
EcoRI GAATTC 2 cut(s) 200, 470
EcoT38I GRGCYC 1 cut(s) 775
FaeI CATG 2 cut(s) 102, 761
FaqI GGGAC 1 cut(s) 149
FatI CATG 2 cut(s) 98, 757
FauI CCCGC 1 cut(s) 52
Fnu4HI GCNGC 8 cut(s) 48, 146, 168, 327, 465, 654, 752, 834
FokI GGATG 3 cut(s) 91, 383, 708
FriOI GRGCYC 1 cut(s) 775
Fsp4HI GCNGC 8 cut(s) 48, 146, 168, 327, 465, 654, 752, 834
FspBI CTAG 3 cut(s) 222, 252, 561
FspI TGCGCA 1 cut(s) 330
GlaI GCGC 1 cut(s) 330
GluI GCNGC 8 cut(s) 48, 146, 168, 327, 465, 654, 752, 834
HhaI GCGC 1 cut(s) 331
Hin1II CATG 2 cut(s) 102, 761
Hin6I GCGC 1 cut(s) 329
HinP1I GCGC 1 cut(s) 329
HinfI GANTC 7 cut(s) 65, 83, 119, 137, 419, 573, 644
Hpy166II GTNNAC 2 cut(s) 273, 829
Hpy188I TCNGA 7 cut(s) 142, 337, 418, 499, 549, 649, 703
Hpy188III TCNNGA 6 cut(s) 62, 266, 307, 403, 570, 577
Hpy8I GTNNAC 2 cut(s) 273, 829
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 2 cut(s) 444, 616
HpyCH4III ACNGT 2 cut(s) 740, 795
HpyCH4V TGCA 5 cut(s) 458, 609, 836, 878, 883
HpyF10VI GCNNNNNNNGC 1 cut(s) 464
HpyF3I CTNAG 2 cut(s) 141, 798
Hsp92II CATG 2 cut(s) 102, 761
HspAI GCGC 1 cut(s) 329
Kzo9I GATC 6 cut(s) 16, 31, 157, 379, 589, 631
LmnI GCTCC 1 cut(s) 770
LpnPI CCDG 7 cut(s) 47, 61, 79, 393, 546, 555, 606
Lsp1109I GCAGC 8 cut(s) 34, 157, 179, 313, 451, 640, 763, 820
LweI GCATC 2 cut(s) 271, 640
MaeI CTAG 3 cut(s) 222, 252, 561
MalI GATC 6 cut(s) 18, 33, 159, 381, 591, 633
MboI GATC 6 cut(s) 16, 31, 157, 379, 589, 631
MboII GAAGA 6 cut(s) 65, 114, 152, 386, 391, 526
MflI RGATCY 3 cut(s) 31, 157, 589
MhlI GDGCHC 1 cut(s) 775
MluCI AATT 4 cut(s) 200, 470, 580, 837
MlyI GAGTC 4 cut(s) 92, 113, 131, 638
MmeI TCCRAC 1 cut(s) 814
MnlI CCTC 6 cut(s) 543, 555, 699, 724, 727, 774
MroXI GAANNNNTTC 1 cut(s) 57
MslI CAYNNNNRTG 1 cut(s) 355
MspA1I CMGCKG 1 cut(s) 47
Mva1269I GAATGC 2 cut(s) 813, 883
MwoI GCNNNNNNNGC 1 cut(s) 464
NdeII GATC 6 cut(s) 16, 31, 157, 379, 589, 631
NlaIII CATG 2 cut(s) 102, 761
NlaIV GGNNCC 2 cut(s) 178, 591
NsbI TGCGCA 1 cut(s) 330
PctI GAATGC 2 cut(s) 813, 883
PdmI GAANNNNTTC 1 cut(s) 57
PfeI GAWTC 3 cut(s) 65, 419, 573
PflMI CCANNNNNTGG 1 cut(s) 186
PkrI GCNGC 8 cut(s) 49, 147, 169, 328, 466, 655, 753, 835
PleI GAGTC 4 cut(s) 91, 113, 131, 638
PpsI GAGTC 4 cut(s) 91, 113, 131, 638
Psp124BI GAGCTC 1 cut(s) 775
PspN4I GGNNCC 2 cut(s) 178, 591
PspPI GGNCC 1 cut(s) 410
PsuI RGATCY 3 cut(s) 31, 157, 589
RsaI GTAC 2 cut(s) 92, 558
RsaNI GTAC 2 cut(s) 91, 557
RseI CAYNNNNRTG 1 cut(s) 355
SacI GAGCTC 1 cut(s) 775
SatI GCNGC 8 cut(s) 48, 146, 168, 327, 465, 654, 752, 834
Sau3AI GATC 6 cut(s) 16, 31, 157, 379, 589, 631
Sau96I GGNCC 1 cut(s) 410
SchI GAGTC 4 cut(s) 92, 113, 131, 638
SduI GDGCHC 1 cut(s) 775
SfaNI GCATC 2 cut(s) 271, 640
SfcI CTRYAG 1 cut(s) 776
SinI GGWCC 1 cut(s) 410
SmiMI CAYNNNNRTG 1 cut(s) 355
SmlI CTYRAG 2 cut(s) 291, 317
SmoI CTYRAG 2 cut(s) 291, 317
Sse9I AATT 4 cut(s) 200, 470, 580, 837
SsiI CCGC 2 cut(s) 45, 491
SspMI CTAG 3 cut(s) 222, 252, 561
SstI GAGCTC 1 cut(s) 775
TaaI ACNGT 2 cut(s) 740, 795
TaqI TCGA 4 cut(s) 299, 306, 541, 805
TasI AATT 4 cut(s) 200, 470, 580, 837
TfiI GAWTC 3 cut(s) 65, 419, 573
TscAI CASTG 3 cut(s) 481, 540, 885
TseI GCWGC 8 cut(s) 47, 145, 167, 326, 464, 653, 751, 833
TspDTI ATGAA 7 cut(s) 140, 213, 261, 387, 704, 746, 759
TspRI CASTG 3 cut(s) 481, 540, 885
Van91I CCANNNNNTGG 1 cut(s) 186
VpaK11BI GGWCC 1 cut(s) 410
XapI RAATTY 2 cut(s) 200, 470
XmnI GAANNNNTTC 1 cut(s) 57
XspI CTAG 3 cut(s) 222, 252, 561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.