Rroxscaffold_7G00159550

Syntaxin 6, N-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010278
Physical Location & Seq
Forward (+)
2726487 .. 2729114
2628 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00159550.1

Sequence Viewer

Length: 960 bp
ATGGCGTCGAGTTTTGATCGGTGGGAGAAGGATCCATATTTCTCGGCGGCCGAGCAAGTTCAAGAATCTGCCGACAGGATGGAATCGACATATAGAACATGGATTCACGCCAAGAAAGACAGTTCCAGTATGTGGAATCCGGAAGAACTCTGCAGGGATCTCCGCACTACTCTTGGAACTACCAAATGGCAGTTAGATGAGTTTGCACGAGCAGTCAGGGCAAGTTATGCTAGAGGCCCGAGTGAAGACGCAAGAGACAGGCACCGCGAATTCATTGTTGCTATGGAGGAACAGATATCTAAAGTTGAAAATGCATTACGGGAATCTGCTTTTTCTGAGGGCAAGACTTCTCTGCCTTGGGTGCGTTTGGACGAAGGAGAGTGTAACGAACTAGCTTCTTTTCTTTCTGGGCTGACTGCTTCTCAAGAGAAAGTTCTTGCTAAGAGTAAGGATATTGAAAACCTACAAGTGACGGATAGAGAATCAGCACCAGATTGTTCAAAGAACTCATGTGGTTCAGGGGGTTCCCGGGAGGCTAGGGAGGAGAAGTTACATGGGCATAGGAGGACAGCTAGTGCTAGTGCTGATATTGTTTCCTGGAAGATTGCGGTATATGATGATGGTCACTTGCCATCTTCGTCTTCTATGATGATTGAACAACCTGTGCGAAAGATACCCAGTTTCTCTGGGTTTATTAGTTCCATGGAATCTGCATCGAAGTTGAAGTGGTCAAAGAATGGTTTTAGGAAGTGGAAGGCAGTGGGCCGTCATGAAGACACTGATGCTGCATTGTTACCATCTACTCAGTTGACTAGGGCATTGATGCTTGCTATGAAAGAAGCAAGAGCTGTTTGGATAGTGATTGCTATGATAAACAATTACATGGCTGGTATGGAGCTATACAGAGACAACTTCAACGATCTCAGTACTATGTGCAGTATAGTTGGCCTGCTCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Syntaxin-6_N

Protein Analysis

319

Amino Acids

35.95

Weight (kDa)

5.97

Isoelectric Point (pI)

40.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
STX6_10_61_N PF09177 11 - 103 2.5e-20 Syntaxin 6/10/61, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 261
AccB7I CCANNNNNTGG 1 cut(s) 132
AccII CGCG 1 cut(s) 267
AccIII TCCGGA 1 cut(s) 139
AciI CCGC 4 cut(s) 47, 163, 265, 608
AclWI GGATC 3 cut(s) 26, 39, 165
AcoI YGGCCR 1 cut(s) 48
AcsI RAATTY 1 cut(s) 269
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 928
AfiI CCNNNNNNNGG 1 cut(s) 132
AgsI TTSAA 7 cut(s) 62, 308, 458, 501, 656, 724, 916
AjnI CCWGG 1 cut(s) 596
AluBI AGCT 4 cut(s) 395, 572, 848, 898
AluI AGCT 4 cut(s) 395, 572, 848, 898
Alw26I GTCTC 2 cut(s) 249, 900
AlwI GGATC 3 cut(s) 26, 39, 165
Ama87I CYCGRG 2 cut(s) 238, 528
Aor13HI TCCGGA 1 cut(s) 139
AoxI GGCC 4 cut(s) 48, 235, 763, 946
ApeKI GCWGC 1 cut(s) 785
ApoI RAATTY 1 cut(s) 269
AspS9I GGNCC 2 cut(s) 236, 763
AsuC2I CCSGG 2 cut(s) 529, 530
AvaI CYCGRG 2 cut(s) 238, 528
BamHI GGATCC 1 cut(s) 31
BanI GGYRCC 1 cut(s) 261
BauI CACGAG 1 cut(s) 207
BbsI GAAGAC 3 cut(s) 252, 633, 780
BbvI GCAGC 1 cut(s) 772
BccI CCATC 4 cut(s) 73, 614, 640, 805
BceAI ACGGC 1 cut(s) 750
BciT130I CCWGG 1 cut(s) 598
BcnI CCSGG 2 cut(s) 529, 530
BcoDI GTCTC 2 cut(s) 249, 900
BfaI CTAG 6 cut(s) 231, 392, 537, 573, 579, 813
BfmI CTRYAG 1 cut(s) 151
BisI GCNGC 2 cut(s) 48, 786
BlsI GCNGC 2 cut(s) 49, 787
BmcAI AGTACT 1 cut(s) 928
Bme1390I CCNGG 3 cut(s) 529, 530, 598
BmeT110I CYCGRG 2 cut(s) 238, 528
BmgT120I GGNCC 2 cut(s) 236, 763
BmiI GGNNCC 3 cut(s) 33, 263, 526
BmrFI CCNGG 3 cut(s) 529, 530, 598
BmrI ACTGGG 1 cut(s) 672
BmsI GCATC 3 cut(s) 722, 772, 813
BmuI ACTGGG 1 cut(s) 672
BpiI GAAGAC 3 cut(s) 252, 633, 780
BpuEI CTTGAG 1 cut(s) 408
BpuMI CCSGG 2 cut(s) 529, 530
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 356, 528, 702
BsaWI WCCGGW 1 cut(s) 139
BsaXI ACNNNNNCTCC 2 cut(s) 533, 563
Bsc4I CCNNNNNNNGG 1 cut(s) 132
Bse1I ACTGG 2 cut(s) 126, 678
BseAI TCCGGA 1 cut(s) 139
BseBI CCWGG 1 cut(s) 598
BseDI CCNNGG 3 cut(s) 356, 528, 702
BseGI GGATG 1 cut(s) 84
BseLI CCNNNNNNNGG 1 cut(s) 132
BseMII CTCAG 3 cut(s) 327, 818, 937
BseNI ACTGG 2 cut(s) 126, 678
BseRI GAGGAG 1 cut(s) 557
BseX3I CGGCCG 1 cut(s) 48
BseXI GCAGC 1 cut(s) 772
BsgI GTGCAG 1 cut(s) 955
Bsh1236I CGCG 1 cut(s) 267
Bsh1285I CGRYCG 1 cut(s) 51
BshFI GGCC 4 cut(s) 50, 237, 765, 948
BshNI GGYRCC 1 cut(s) 261
BsiEI CGRYCG 1 cut(s) 51
BsiHKCI CYCGRG 2 cut(s) 238, 528
BsiSI CCGG 2 cut(s) 140, 529
BslI CCNNNNNNNGG 1 cut(s) 132
BsmAI GTCTC 2 cut(s) 249, 900
BsnI GGCC 4 cut(s) 50, 237, 765, 948
BsoBI CYCGRG 2 cut(s) 238, 528
Bsp13I TCCGGA 1 cut(s) 139
Bsp143I GATC 4 cut(s) 16, 31, 157, 919
Bsp19I CCATGG 1 cut(s) 702
BspACI CCGC 4 cut(s) 47, 163, 265, 608
BspANI GGCC 4 cut(s) 50, 237, 765, 948
BspCNI CTCAG 3 cut(s) 328, 817, 936
BspEI TCCGGA 1 cut(s) 139
BspFNI CGCG 1 cut(s) 267
BspHI TCATGA 1 cut(s) 769
BspLI GGNNCC 3 cut(s) 33, 263, 526
BspMAI CTGCAG 1 cut(s) 155
BspPI GGATC 3 cut(s) 26, 39, 165
BspT107I GGYRCC 1 cut(s) 261
BsrI ACTGG 2 cut(s) 126, 678
BssECI CCNNGG 3 cut(s) 356, 528, 702
BssMI GATC 4 cut(s) 16, 31, 157, 919
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 207
BssT1I CCWWGG 2 cut(s) 356, 702
Bst2BI CACGAG 1 cut(s) 207
Bst2UI CCWGG 1 cut(s) 598
Bst4CI ACNGT 1 cut(s) 122
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 227
BstC8I GCNNGC 2 cut(s) 828, 950
BstDEI CTNAG 4 cut(s) 336, 441, 804, 923
BstDSI CCRYGG 1 cut(s) 702
BstF5I GGATG 1 cut(s) 84
BstFNI CGCG 1 cut(s) 267
BstKTI GATC 4 cut(s) 19, 34, 160, 922
BstMAI GTCTC 2 cut(s) 249, 900
BstMBI GATC 4 cut(s) 16, 31, 157, 919
BstMCI CGRYCG 1 cut(s) 51
BstMWI GCNNNNNNNGC 3 cut(s) 218, 227, 361
BstNI CCWGG 1 cut(s) 598
BstSCI CCNGG 3 cut(s) 527, 528, 596
BstSFI CTRYAG 1 cut(s) 151
BstUI CGCG 1 cut(s) 267
BstV1I GCAGC 1 cut(s) 772
BstV2I GAAGAC 3 cut(s) 252, 633, 780
BstX2I RGATCY 2 cut(s) 31, 157
BstYI RGATCY 2 cut(s) 31, 157
BstZI CGGCCG 1 cut(s) 48
BsuRI GGCC 4 cut(s) 50, 237, 765, 948
BtgI CCRYGG 1 cut(s) 702
BtsCI GGATG 1 cut(s) 84
BtsI GCAGTG 1 cut(s) 765
BtsIMutI CAGTG 2 cut(s) 765, 777
Cac8I GCNNGC 2 cut(s) 828, 950
CciI TCATGA 1 cut(s) 769
Cfr13I GGNCC 2 cut(s) 236, 763
Cfr9I CCCGGG 1 cut(s) 528
CseI GACGC 1 cut(s) 257
Csp6I GTAC 1 cut(s) 927
CviAII CATG 7 cut(s) 99, 510, 554, 703, 770, 883, 955
CviQI GTAC 1 cut(s) 927
DdeI CTNAG 4 cut(s) 336, 441, 804, 923
DpnI GATC 4 cut(s) 18, 33, 159, 921
DpnII GATC 4 cut(s) 16, 31, 157, 919
EaeI YGGCCR 1 cut(s) 48
EagI CGGCCG 1 cut(s) 48
EclXI CGGCCG 1 cut(s) 48
Eco130I CCWWGG 2 cut(s) 356, 702
Eco32I GATATC 1 cut(s) 297
Eco52I CGGCCG 1 cut(s) 48
Eco88I CYCGRG 2 cut(s) 238, 528
EcoRI GAATTC 1 cut(s) 269
EcoRII CCWGG 1 cut(s) 596
EcoRV GATATC 1 cut(s) 297
EcoT14I CCWWGG 2 cut(s) 356, 702
EcoT22I ATGCAT 1 cut(s) 316
ErhI CCWWGG 2 cut(s) 356, 702
FaeI CATG 7 cut(s) 102, 513, 557, 706, 773, 886, 958
FatI CATG 7 cut(s) 98, 509, 553, 702, 769, 882, 954
Fnu4HI GCNGC 2 cut(s) 48, 786
FokI GGATG 1 cut(s) 91
Fsp4HI GCNGC 2 cut(s) 48, 786
FspBI CTAG 6 cut(s) 231, 392, 537, 573, 579, 813
GluI GCNGC 2 cut(s) 48, 786
HaeIII GGCC 4 cut(s) 50, 237, 765, 948
HapII CCGG 2 cut(s) 140, 529
HgaI GACGC 1 cut(s) 257
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 7 cut(s) 102, 513, 557, 706, 773, 886, 958
HincII GTYRAC 1 cut(s) 810
HindII GTYRAC 1 cut(s) 810
HinfI GANTC 7 cut(s) 65, 83, 103, 136, 323, 482, 707
HpaII CCGG 2 cut(s) 140, 529
Hpy166II GTNNAC 1 cut(s) 810
Hpy188I TCNGA 1 cut(s) 337
Hpy188III TCNNGA 4 cut(s) 62, 140, 425, 770
Hpy8I GTNNAC 1 cut(s) 810
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 3 cut(s) 22, 368, 748
HpyCH4III ACNGT 1 cut(s) 122
HpyCH4V TGCA 6 cut(s) 153, 206, 314, 713, 788, 936
HpyF10VI GCNNNNNNNGC 3 cut(s) 218, 227, 361
HpyF3I CTNAG 4 cut(s) 336, 441, 804, 923
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 7 cut(s) 102, 513, 557, 706, 773, 886, 958
Kpn2I TCCGGA 1 cut(s) 139
Kzo9I GATC 4 cut(s) 16, 31, 157, 919
LmnI GCTCC 1 cut(s) 895
Lsp1109I GCAGC 1 cut(s) 772
LweI GCATC 3 cut(s) 722, 772, 813
MaeI CTAG 6 cut(s) 231, 392, 537, 573, 579, 813
MaeIII GTNAC 5 cut(s) 383, 469, 549, 623, 792
MalI GATC 4 cut(s) 18, 33, 159, 921
MboI GATC 4 cut(s) 16, 31, 157, 919
MboII GAAGA 6 cut(s) 155, 257, 613, 627, 633, 785
MflI RGATCY 2 cut(s) 31, 157
MluCI AATT 2 cut(s) 269, 877
MnlI CCTC 6 cut(s) 227, 280, 331, 526, 535, 558
Mph1103I ATGCAT 1 cut(s) 316
MroI TCCGGA 1 cut(s) 139
MspI CCGG 2 cut(s) 140, 529
MspR9I CCNGG 3 cut(s) 529, 530, 598
MvaI CCWGG 1 cut(s) 598
MvnI CGCG 1 cut(s) 267
MwoI GCNNNNNNNGC 3 cut(s) 218, 227, 361
NciI CCSGG 2 cut(s) 529, 530
NcoI CCATGG 1 cut(s) 702
NdeII GATC 4 cut(s) 16, 31, 157, 919
NlaIII CATG 7 cut(s) 102, 513, 557, 706, 773, 886, 958
NlaIV GGNNCC 3 cut(s) 33, 263, 526
NmeAIII GCCGAG 2 cut(s) 23, 76
NmuCI GTSAC 2 cut(s) 469, 623
NsiI ATGCAT 1 cut(s) 316
PagI TCATGA 1 cut(s) 769
PfeI GAWTC 7 cut(s) 65, 83, 103, 136, 323, 482, 707
PflMI CCANNNNNTGG 1 cut(s) 132
PfoI TCCNGGA 1 cut(s) 596
PkrI GCNGC 2 cut(s) 49, 787
Psp6I CCWGG 1 cut(s) 596
PspGI CCWGG 1 cut(s) 596
PspN4I GGNNCC 3 cut(s) 33, 263, 526
PspPI GGNCC 2 cut(s) 236, 763
PstI CTGCAG 1 cut(s) 155
PsuI RGATCY 2 cut(s) 31, 157
RsaI GTAC 1 cut(s) 928
RsaNI GTAC 1 cut(s) 927
SatI GCNGC 2 cut(s) 48, 786
Sau3AI GATC 4 cut(s) 16, 31, 157, 919
Sau96I GGNCC 2 cut(s) 236, 763
ScaI AGTACT 1 cut(s) 928
ScrFI CCNGG 3 cut(s) 529, 530, 598
SetI ASST 6 cut(s) 397, 465, 574, 664, 850, 900
SfaNI GCATC 3 cut(s) 722, 772, 813
SfcI CTRYAG 1 cut(s) 151
SmaI CCCGGG 1 cut(s) 530
SmlI CTYRAG 1 cut(s) 423
SmoI CTYRAG 1 cut(s) 423
Sse9I AATT 2 cut(s) 269, 877
SsiI CCGC 4 cut(s) 47, 163, 265, 608
SspMI CTAG 6 cut(s) 231, 392, 537, 573, 579, 813
StyD4I CCNGG 3 cut(s) 527, 528, 596
StyI CCWWGG 2 cut(s) 356, 702
TaaI ACNGT 1 cut(s) 122
TaqI TCGA 3 cut(s) 8, 86, 716
TasI AATT 2 cut(s) 269, 877
TatI WGTACW 1 cut(s) 926
TauI GCSGC 1 cut(s) 50
TfiI GAWTC 7 cut(s) 65, 83, 103, 136, 323, 482, 707
TscAI CASTG 2 cut(s) 765, 784
TseFI GTSAC 2 cut(s) 469, 623
TseI GCWGC 1 cut(s) 785
Tsp45I GTSAC 2 cut(s) 469, 623
TspDTI ATGAA 3 cut(s) 262, 786, 848
TspGWI ACGGA 1 cut(s) 488
TspMI CCCGGG 1 cut(s) 528
TspRI CASTG 2 cut(s) 765, 784
Van91I CCANNNNNTGG 1 cut(s) 132
XapI RAATTY 1 cut(s) 269
XmaI CCCGGG 1 cut(s) 528
XspI CTAG 6 cut(s) 231, 392, 537, 573, 579, 813
ZrmI AGTACT 1 cut(s) 928
Zsp2I ATGCAT 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.