MD15G1073000.v1.1

Syntaxin 6, N-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010278
Physical Location & Seq
Forward (+)
4975212 .. 4977716
2505 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1073000.v1.1.491

Sequence Viewer

Length: 1038 bp
ATGACGTCAGGTTTTGATCGGTGGGAGAAAGATCCATTTTTCCCGGCGGCCGAGGAAGTTCAAGATTCCGCCGACAGGATGGAATCTACACATCGAACATGGATCCACGCCAGGAAAGACGCTTCCAGTATGTGGGATCCCGAGGAACTTGGCAGGGATCTTCGTACAACTCTTGGAACTACCAAATGGCAGTTGGAGGAGTTTGTACGAGCAGTCAGGTCAAGTTATGCGAGAAGGTCATGTGAAGACGCAAGAGACAGGCACCATCAATTCATTGTTGCCATCGAGGAGCAGGTTTCCAAAATTGAAAAATCGTTAAAGGAGGCTGCCCTTTCTGAGGGCAAGGCTACTCAACCTTGGGTGCGTTTGGATGAAGGGGAGTGCAATGAACTAGCGTTTTTTCTTTCTGGGCCATCTGCATCTGAAGAGAAAGTTATTGCCAAGGGTAACGACAGGGATTCTGAAAACCCACGAGTGACAGATGGAGATACGGCACCAGGTAGTTCGAAGAACTCATGTGGTTCAAACGAGCGGGGCTCCCAGAAGACTAGGGAGGAGAAACCACATGGGCATAGGAGGACAGCTAGCGCTAGTGCTGACATTGGTGCCTTGAAGATTGCAGTTTTCGACGATGGATACGTACCAAGTTCTTCTGCTATGATGGTTGAGCATCCTGTACGAAAGATTCCAAGTATGTCTGGGTTTCTCAGTTCCATGGAAACCGCATCAAAGTTGAAGTGGTCAAAGAATGGTTTTAGGAAGTGGAAGGCAGTGGACCGTCATGAAGACGCTGATGCTGCATTGTTGCCATCTACTCAGTTGACCAGGGGCGTCAATGCTTGCTTTGAAAAAAATAAGAGTTGTTTGGATAGTTGCGATGAATGTTACGATAAACCATTGTATGGCTGGTACGGAGCTATTCAAAGACAACTTCAAAGGTCTCAGTACCACATGCAATATAGTTGGCCTGCCCGCATAGCTCTTTGGATTGTTATTCTCCTTTTCTTGATTGTTTTAATTGCATTGCGTGCGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Syntaxin-6_N

Protein Analysis

346

Amino Acids

38.98

Weight (kDa)

6.53

Isoelectric Point (pI)

51.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
STX6_10_61_N PF09177 11 - 103 8.8e-20 Syntaxin 6/10/61, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 8
Acc36I ACCTGC 1 cut(s) 283
AccB1I GGYRCC 3 cut(s) 261, 493, 605
AccB7I CCANNNNNTGG 2 cut(s) 132, 902
AccBSI CCGCTC 1 cut(s) 532
AciI CCGC 5 cut(s) 47, 69, 532, 723, 973
AclWI GGATC 6 cut(s) 26, 97, 110, 131, 144, 165
AcoI YGGCCR 1 cut(s) 48
AcuI CTGAAG 1 cut(s) 444
AcyI GRCGYC 2 cut(s) 5, 831
AfaI GTAC 6 cut(s) 166, 207, 642, 678, 911, 947
AfeI AGCGCT 1 cut(s) 589
AfiI CCNNNNNNNGG 4 cut(s) 75, 132, 337, 902
AgsI TTSAA 8 cut(s) 62, 308, 525, 613, 736, 848, 923, 935
AjnI CCWGG 3 cut(s) 110, 496, 824
AluBI AGCT 3 cut(s) 584, 917, 980
AluI AGCT 3 cut(s) 584, 917, 980
Alw26I GTCTC 2 cut(s) 249, 945
AlwI GGATC 6 cut(s) 26, 97, 110, 131, 144, 165
Ama87I CYCGRG 1 cut(s) 140
Aor51HI AGCGCT 1 cut(s) 589
AoxI GGCC 3 cut(s) 48, 410, 965
ApeKI GCWGC 2 cut(s) 326, 797
AspLEI GCGC 1 cut(s) 590
AspS9I GGNCC 2 cut(s) 410, 775
AsuC2I CCSGG 1 cut(s) 44
AsuII TTCGAA 1 cut(s) 506
AsuNHI GCTAGC 1 cut(s) 584
AvaI CYCGRG 1 cut(s) 140
AvaII GGWCC 1 cut(s) 775
BamHI GGATCC 2 cut(s) 102, 136
BanI GGYRCC 3 cut(s) 261, 493, 605
BanII GRGCYC 1 cut(s) 539
BauI CACGAG 1 cut(s) 471
BbsI GAAGAC 3 cut(s) 252, 551, 792
BbvI GCAGC 2 cut(s) 313, 784
BccI CCATC 8 cut(s) 73, 273, 290, 421, 476, 626, 655, 817
BceAI ACGGC 1 cut(s) 507
BciT130I CCWGG 3 cut(s) 112, 498, 826
BciVI GTATCC 1 cut(s) 629
BcnI CCSGG 1 cut(s) 44
BcoDI GTCTC 2 cut(s) 249, 945
BfaI CTAG 4 cut(s) 392, 549, 585, 591
BfoI RGCGCY 1 cut(s) 591
BfuAI ACCTGC 1 cut(s) 283
BfuI GTATCC 1 cut(s) 629
BisI GCNGC 3 cut(s) 48, 327, 798
BlsI GCNGC 3 cut(s) 49, 328, 799
Bme1390I CCNGG 4 cut(s) 44, 112, 498, 826
Bme18I GGWCC 1 cut(s) 775
BmeT110I CYCGRG 1 cut(s) 140
BmgT120I GGNCC 2 cut(s) 410, 775
BmiI GGNNCC 6 cut(s) 104, 138, 263, 495, 538, 607
BmrFI CCNGG 4 cut(s) 44, 112, 498, 826
BmsI GCATC 4 cut(s) 428, 679, 734, 784
BmtI GCTAGC 1 cut(s) 588
BpiI GAAGAC 3 cut(s) 252, 551, 792
BplI GAGNNNNNCTC 2 cut(s) 521, 553
Bpu14I TTCGAA 1 cut(s) 506
BpuMI CCSGG 1 cut(s) 44
BsaAI YACGTR 1 cut(s) 640
BsaHI GRCGYC 2 cut(s) 5, 831
BsaI GGTCTC 1 cut(s) 945
BsaJI CCNNGG 6 cut(s) 51, 141, 356, 441, 714, 825
Bsc4I CCNNNNNNNGG 4 cut(s) 75, 132, 337, 902
Bse1I ACTGG 1 cut(s) 126
Bse3DI GCAATG 2 cut(s) 391, 1022
BseBI CCWGG 3 cut(s) 112, 498, 826
BseDI CCNNGG 6 cut(s) 51, 141, 356, 441, 714, 825
BseGI GGATG 3 cut(s) 84, 376, 670
BseLI CCNNNNNNNGG 4 cut(s) 75, 132, 337, 902
BseMI GCAATG 2 cut(s) 391, 1022
BseMII CTCAG 4 cut(s) 327, 721, 830, 956
BseNI ACTGG 1 cut(s) 126
BseRI GAGGAG 3 cut(s) 212, 302, 569
BseX3I CGGCCG 1 cut(s) 48
BseXI GCAGC 2 cut(s) 313, 784
Bsh1285I CGRYCG 1 cut(s) 51
BshFI GGCC 3 cut(s) 50, 412, 967
BshNI GGYRCC 3 cut(s) 261, 493, 605
BsiEI CGRYCG 1 cut(s) 51
BsiHKCI CYCGRG 1 cut(s) 140
BsiSI CCGG 1 cut(s) 44
BslI CCNNNNNNNGG 4 cut(s) 75, 132, 337, 902
BsmAI GTCTC 2 cut(s) 249, 945
BsnI GGCC 3 cut(s) 50, 412, 967
Bso31I GGTCTC 1 cut(s) 945
BsoBI CYCGRG 1 cut(s) 140
Bsp119I TTCGAA 1 cut(s) 506
Bsp1286I GDGCHC 1 cut(s) 539
Bsp143I GATC 6 cut(s) 16, 31, 102, 136, 157, 1032
Bsp19I CCATGG 1 cut(s) 714
BspACI CCGC 5 cut(s) 47, 69, 532, 723, 973
BspANI GGCC 3 cut(s) 50, 412, 967
BspCNI CTCAG 4 cut(s) 328, 720, 829, 955
BspHI TCATGA 1 cut(s) 781
BspLI GGNNCC 6 cut(s) 104, 138, 263, 495, 538, 607
BspMI ACCTGC 1 cut(s) 283
BspOI GCTAGC 1 cut(s) 588
BspPI GGATC 6 cut(s) 26, 97, 110, 131, 144, 165
BspT104I TTCGAA 1 cut(s) 506
BspT107I GGYRCC 3 cut(s) 261, 493, 605
BspTNI GGTCTC 1 cut(s) 945
BsrBI CCGCTC 1 cut(s) 532
BsrDI GCAATG 2 cut(s) 391, 1022
BsrI ACTGG 1 cut(s) 126
BssECI CCNNGG 6 cut(s) 51, 141, 356, 441, 714, 825
BssMI GATC 6 cut(s) 16, 31, 102, 136, 157, 1032
BssNI GRCGYC 2 cut(s) 5, 831
BssSI CACGAG 1 cut(s) 471
BssT1I CCWWGG 3 cut(s) 356, 441, 714
Bst2BI CACGAG 1 cut(s) 471
Bst2UI CCWGG 3 cut(s) 112, 498, 826
Bst4CI ACNGT 1 cut(s) 779
Bst6I CTCTTC 1 cut(s) 420
BstACI GRCGYC 2 cut(s) 5, 831
BstAPI GCANNNNNTGC 1 cut(s) 1028
BstBAI YACGTR 1 cut(s) 640
BstBI TTCGAA 1 cut(s) 506
BstC8I GCNNGC 5 cut(s) 586, 841, 969, 973, 1029
BstDEI CTNAG 4 cut(s) 336, 707, 816, 942
BstDSI CCRYGG 1 cut(s) 714
BstENI CCTNNNNNAGG 1 cut(s) 335
BstF5I GGATG 3 cut(s) 84, 376, 670
BstH2I RGCGCY 1 cut(s) 591
BstHHI GCGC 1 cut(s) 590
BstKTI GATC 6 cut(s) 19, 34, 105, 139, 160, 1035
BstMAI GTCTC 2 cut(s) 249, 945
BstMBI GATC 6 cut(s) 16, 31, 102, 136, 157, 1032
BstMCI CGRYCG 1 cut(s) 51
BstMWI GCNNNNNNNGC 3 cut(s) 797, 977, 1028
BstNI CCWGG 3 cut(s) 112, 498, 826
BstNSI RCATGY 1 cut(s) 955
BstSCI CCNGG 4 cut(s) 42, 110, 496, 824
BstSNI TACGTA 1 cut(s) 640
BstV1I GCAGC 2 cut(s) 313, 784
BstV2I GAAGAC 3 cut(s) 252, 551, 792
BstX2I RGATCY 4 cut(s) 31, 102, 136, 157
BstYI RGATCY 4 cut(s) 31, 102, 136, 157
BstZI CGGCCG 1 cut(s) 48
BsuI GTATCC 1 cut(s) 629
BsuRI GGCC 3 cut(s) 50, 412, 967
BtgI CCRYGG 1 cut(s) 714
BtgZI GCGATG 1 cut(s) 891
BtsCI GGATG 3 cut(s) 84, 376, 670
BtsI GCAGTG 1 cut(s) 777
BtsIMutI CAGTG 1 cut(s) 777
BveI ACCTGC 1 cut(s) 283
Cac8I GCNNGC 5 cut(s) 586, 841, 969, 973, 1029
CciI TCATGA 1 cut(s) 781
CfoI GCGC 1 cut(s) 590
Cfr13I GGNCC 2 cut(s) 410, 775
CseI GACGC 4 cut(s) 128, 257, 797, 820
CsiI ACCWGGT 1 cut(s) 496
Csp6I GTAC 6 cut(s) 165, 206, 641, 677, 910, 946
CviAII CATG 7 cut(s) 99, 240, 516, 566, 715, 782, 952
CviQI GTAC 6 cut(s) 165, 206, 641, 677, 910, 946
DdeI CTNAG 4 cut(s) 336, 707, 816, 942
DpnI GATC 6 cut(s) 18, 33, 104, 138, 159, 1034
DpnII GATC 6 cut(s) 16, 31, 102, 136, 157, 1032
EaeI YGGCCR 1 cut(s) 48
EagI CGGCCG 1 cut(s) 48
Eam1104I CTCTTC 1 cut(s) 420
EarI CTCTTC 1 cut(s) 420
EciI GGCGGA 1 cut(s) 58
EclXI CGGCCG 1 cut(s) 48
Eco105I TACGTA 1 cut(s) 640
Eco130I CCWWGG 3 cut(s) 356, 441, 714
Eco24I GRGCYC 1 cut(s) 539
Eco31I GGTCTC 1 cut(s) 945
Eco47I GGWCC 1 cut(s) 775
Eco47III AGCGCT 1 cut(s) 589
Eco52I CGGCCG 1 cut(s) 48
Eco57I CTGAAG 1 cut(s) 444
Eco88I CYCGRG 1 cut(s) 140
EcoNI CCTNNNNNAGG 1 cut(s) 335
EcoRII CCWGG 3 cut(s) 110, 496, 824
EcoT14I CCWWGG 3 cut(s) 356, 441, 714
EcoT38I GRGCYC 1 cut(s) 539
ErhI CCWWGG 3 cut(s) 356, 441, 714
FaeI CATG 7 cut(s) 102, 243, 519, 569, 718, 785, 955
FatI CATG 7 cut(s) 98, 239, 515, 565, 714, 781, 951
FauI CCCGC 2 cut(s) 525, 980
Fnu4HI GCNGC 3 cut(s) 48, 327, 798
FokI GGATG 3 cut(s) 91, 383, 657
FriOI GRGCYC 1 cut(s) 539
Fsp4HI GCNGC 3 cut(s) 48, 327, 798
FspBI CTAG 4 cut(s) 392, 549, 585, 591
GlaI GCGC 1 cut(s) 589
GluI GCNGC 3 cut(s) 48, 327, 798
HaeII RGCGCY 1 cut(s) 591
HaeIII GGCC 3 cut(s) 50, 412, 967
HapII CCGG 1 cut(s) 44
HgaI GACGC 4 cut(s) 128, 257, 797, 820
HhaI GCGC 1 cut(s) 590
Hin1I GRCGYC 2 cut(s) 5, 831
Hin1II CATG 7 cut(s) 102, 243, 519, 569, 718, 785, 955
Hin6I GCGC 1 cut(s) 588
HinP1I GCGC 1 cut(s) 588
HincII GTYRAC 1 cut(s) 822
HindII GTYRAC 1 cut(s) 822
HinfI GANTC 4 cut(s) 65, 83, 458, 685
HpaII CCGG 1 cut(s) 44
Hpy166II GTNNAC 2 cut(s) 775, 822
Hpy188I TCNGA 4 cut(s) 337, 424, 463, 1037
Hpy188III TCNNGA 4 cut(s) 62, 140, 782, 1006
Hpy8I GTNNAC 2 cut(s) 775, 822
Hpy99I CGWCG 1 cut(s) 632
HpyAV CCTTC 3 cut(s) 228, 368, 760
HpyCH4III ACNGT 1 cut(s) 779
HpyCH4IV ACGT 2 cut(s) 5, 639
HpyCH4V TGCA 6 cut(s) 384, 419, 620, 800, 955, 1022
HpyF10VI GCNNNNNNNGC 3 cut(s) 797, 977, 1028
HpyF3I CTNAG 4 cut(s) 336, 707, 816, 942
HpySE526I ACGT 2 cut(s) 5, 639
Hsp92I GRCGYC 2 cut(s) 5, 831
Hsp92II CATG 7 cut(s) 102, 243, 519, 569, 718, 785, 955
HspAI GCGC 1 cut(s) 588
Kzo9I GATC 6 cut(s) 16, 31, 102, 136, 157, 1032
LmnI GCTCC 3 cut(s) 289, 542, 914
Lsp1109I GCAGC 2 cut(s) 313, 784
LweI GCATC 4 cut(s) 428, 679, 734, 784
MabI ACCWGGT 1 cut(s) 496
MaeI CTAG 4 cut(s) 392, 549, 585, 591
MaeII ACGT 2 cut(s) 5, 639
MaeIII GTNAC 3 cut(s) 446, 475, 884
MalI GATC 6 cut(s) 18, 33, 104, 138, 159, 1034
MbiI CCGCTC 1 cut(s) 532
MboI GATC 6 cut(s) 16, 31, 102, 136, 157, 1032
MboII GAAGA 8 cut(s) 152, 257, 437, 520, 556, 625, 642, 797
MflI RGATCY 4 cut(s) 31, 102, 136, 157
MhlI GDGCHC 1 cut(s) 539
MluCI AATT 3 cut(s) 269, 303, 1017
MmeI TCCRAC 1 cut(s) 174
MnlI CCTC 8 cut(s) 46, 136, 190, 280, 316, 331, 547, 570
MseI TTAA 2 cut(s) 317, 1016
MspI CCGG 1 cut(s) 44
MspR9I CCNGG 4 cut(s) 44, 112, 498, 826
MvaI CCWGG 3 cut(s) 112, 498, 826
MwoI GCNNNNNNNGC 3 cut(s) 797, 977, 1028
NciI CCSGG 1 cut(s) 44
NcoI CCATGG 1 cut(s) 714
NdeII GATC 6 cut(s) 16, 31, 102, 136, 157, 1032
NheI GCTAGC 1 cut(s) 584
NlaIII CATG 7 cut(s) 102, 243, 519, 569, 718, 785, 955
NlaIV GGNNCC 6 cut(s) 104, 138, 263, 495, 538, 607
NmeAIII GCCGAG 1 cut(s) 76
NmuCI GTSAC 1 cut(s) 475
NspI RCATGY 1 cut(s) 955
NspV TTCGAA 1 cut(s) 506
PagI TCATGA 1 cut(s) 781
PcsI WCGNNNNNNNCGW 1 cut(s) 636
PfeI GAWTC 4 cut(s) 65, 83, 458, 685
PflMI CCANNNNNTGG 2 cut(s) 132, 902
PkrI GCNGC 3 cut(s) 49, 328, 799
Ppu21I YACGTR 1 cut(s) 640
Psp6I CCWGG 3 cut(s) 110, 496, 824
PspGI CCWGG 3 cut(s) 110, 496, 824
PspN4I GGNNCC 6 cut(s) 104, 138, 263, 495, 538, 607
PspPI GGNCC 2 cut(s) 410, 775
PsuI RGATCY 4 cut(s) 31, 102, 136, 157
RsaI GTAC 6 cut(s) 166, 207, 642, 678, 911, 947
RsaNI GTAC 6 cut(s) 165, 206, 641, 677, 910, 946
SaqAI TTAA 2 cut(s) 317, 1016
SatI GCNGC 3 cut(s) 48, 327, 798
Sau3AI GATC 6 cut(s) 16, 31, 102, 136, 157, 1032
Sau96I GGNCC 2 cut(s) 410, 775
ScrFI CCNGG 4 cut(s) 44, 112, 498, 826
SduI GDGCHC 1 cut(s) 539
SexAI ACCWGGT 1 cut(s) 496
SfaNI GCATC 4 cut(s) 428, 679, 734, 784
SfuI TTCGAA 1 cut(s) 506
SinI GGWCC 1 cut(s) 775
SnaBI TACGTA 1 cut(s) 640
Sse9I AATT 3 cut(s) 269, 303, 1017
SsiI CCGC 5 cut(s) 47, 69, 532, 723, 973
SspMI CTAG 4 cut(s) 392, 549, 585, 591
StyD4I CCNGG 4 cut(s) 42, 110, 496, 824
StyI CCWWGG 3 cut(s) 356, 441, 714
TaaI ACNGT 1 cut(s) 779
TaiI ACGT 2 cut(s) 8, 642
TaqI TCGA 4 cut(s) 94, 285, 506, 627
TasI AATT 3 cut(s) 269, 303, 1017
TauI GCSGC 1 cut(s) 50
TfiI GAWTC 4 cut(s) 65, 83, 458, 685
Tru1I TTAA 2 cut(s) 317, 1016
Tru9I TTAA 2 cut(s) 317, 1016
TscAI CASTG 1 cut(s) 777
TseFI GTSAC 1 cut(s) 475
TseI GCWGC 2 cut(s) 326, 797
Tsp45I GTSAC 1 cut(s) 475
TspDTI ATGAA 5 cut(s) 262, 387, 402, 798, 894
TspGWI ACGGA 1 cut(s) 927
TspRI CASTG 1 cut(s) 777
Van91I CCANNNNNTGG 2 cut(s) 132, 902
VpaK11BI GGWCC 1 cut(s) 775
XagI CCTNNNNNAGG 1 cut(s) 335
XceI RCATGY 1 cut(s) 955
XcmI CCANNNNNNNNNTGG 2 cut(s) 190, 903
XspI CTAG 4 cut(s) 392, 549, 585, 591
ZraI GACGTC 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.