MD08G1088200.v1.1

Syntaxin 6, N-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010278
Physical Location & Seq
Forward (+)
7276398 .. 7278864
2467 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1088200.v1.1.491

Sequence Viewer

Length: 1035 bp
ATGGCGTCAAGTTTTGATCGGTGGGAGAAAGATCCATTTTTTCCGGCGGCCGAAGAAGTTCAAGAATCCGCCGACAGGATGGAATCTACTTATCGGACATGGATCCACGCCAGGAAGGACGCTTCCTGTATGTGGGATCCCGAGGAACTTTGCAGGGATCTTCGTACAGCTCTTGGAACTACCAAATGGCAGTTAGAGGAGTTTGCACGAGCAGTCAGGTCGAGTTATGCGAGAAGCTCATGTGAAGACGCAAGAGAGAGGCACCGTGCATTCATTGTTGCGATCGAGGAGCAGGTTTCCAAAATTGAAAAATCGTTAAAGGAAGCTGCCCTTTCTGAGGGCAAGGCTTCTCAGCCTTGGGTGCGTTTGGATGAAGGGGAGTGCAATGAACTTGCATTTTTTCTTTCTGGACCATCTGCTTCCGAAGAGAAAGTTCTTGTTAAAAGTAACGACAAGGATGTTGAAAAACCACGAGTCACAGATGGAGATACGGCGCCAGGTTGTTCAAAGAACTCATGTGGTTCGACCGAGTTGGGCTCCCAGAAGACTAGGGAGGAGAAGTCACATGGGCATAGGAGGACAGCTAGTGATAGTGCTGACGTTGGTGCCTGGAAGATTGCAGTTTTCGATGATGGATACGTACCAAGTTCTTCTGCTATGATGGTTGAGCATCCTGTACGAAAGATTCCTAGTATGTCTGGGTTTCTTAGTTCCATGGAAACTGCGTCAAAGTTGAAGTGGTCAAAGAATGGTTTTAGGAAGTGGAAGGCCGTCGACCGTCATGAAGATGCTGATACTGCATTGTTGCCACCTAGTCAGTTGACTAGGGGCATCAATGCTTGCTTGGAAAAAAATAAGAGTTGTTTGGATAGTTGCGATGAATGCTACGATAAACCGTTGTATGGCTGGTACGGAGCTATCCAGAGACAACTTCAACGGTCTCAGTACTATATGCAATATAGTTGGCCTGCACGCGTAGCACTTTGGATCGGTGTACTCCTTTTCTTGATTGTCTTTGTGTTGCGTGCGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Syntaxin-6_N

Protein Analysis

345

Amino Acids

38.94

Weight (kDa)

6.16

Isoelectric Point (pI)

55.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
STX6_10_61_N PF09177 11 - 103 1.9e-20 Syntaxin 6/10/61, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 283
AccB1I GGYRCC 3 cut(s) 261, 493, 605
AccI GTMKAC 1 cut(s) 774
AccII CGCG 1 cut(s) 975
AciI CCGC 2 cut(s) 47, 69
AclWI GGATC 7 cut(s) 26, 97, 110, 131, 144, 165, 995
AcoI YGGCCR 1 cut(s) 48
AcyI GRCGYC 2 cut(s) 5, 494
AfaI GTAC 6 cut(s) 166, 642, 678, 911, 947, 996
AfiI CCNNNNNNNGG 4 cut(s) 75, 132, 337, 902
AflIII ACRYGT 1 cut(s) 973
AgsI TTSAA 6 cut(s) 62, 308, 464, 507, 736, 935
AjnI CCWGG 3 cut(s) 110, 496, 608
AluBI AGCT 5 cut(s) 170, 237, 326, 584, 917
AluI AGCT 5 cut(s) 170, 237, 326, 584, 917
Alw26I GTCTC 2 cut(s) 919, 945
AlwI GGATC 7 cut(s) 26, 97, 110, 131, 144, 165, 995
Ama87I CYCGRG 1 cut(s) 140
AoxI GGCC 3 cut(s) 48, 768, 965
ApeKI GCWGC 1 cut(s) 326
Asp700I GAANNNNTTC 1 cut(s) 57
AspLEI GCGC 1 cut(s) 496
AspS9I GGNCC 1 cut(s) 410
AvaI CYCGRG 1 cut(s) 140
AvaII GGWCC 1 cut(s) 410
BamHI GGATCC 2 cut(s) 102, 136
BanI GGYRCC 3 cut(s) 261, 493, 605
BanII GRGCYC 1 cut(s) 539
BauI CACGAG 2 cut(s) 207, 471
BbsI GAAGAC 2 cut(s) 252, 551
BbvI GCAGC 1 cut(s) 313
BccI CCATC 5 cut(s) 73, 421, 476, 626, 655
BceAI ACGGC 2 cut(s) 507, 755
BcgI CGANNNNNNTGC 2 cut(s) 201, 235
BciT130I CCWGG 3 cut(s) 112, 498, 610
BciVI GTATCC 1 cut(s) 629
BcoDI GTCTC 2 cut(s) 919, 945
BfaI CTAG 5 cut(s) 549, 585, 690, 813, 825
BfoI RGCGCY 1 cut(s) 497
BfuAI ACCTGC 1 cut(s) 283
BfuI GTATCC 1 cut(s) 629
BisI GCNGC 2 cut(s) 48, 327
BlsI GCNGC 2 cut(s) 49, 328
BmcAI AGTACT 1 cut(s) 947
Bme1390I CCNGG 3 cut(s) 112, 498, 610
Bme18I GGWCC 1 cut(s) 410
BmeT110I CYCGRG 1 cut(s) 140
BmgT120I GGNCC 1 cut(s) 410
BmiI GGNNCC 6 cut(s) 104, 138, 263, 495, 538, 607
BmrFI CCNGG 3 cut(s) 112, 498, 610
BmsI GCATC 3 cut(s) 679, 778, 840
BpiI GAAGAC 2 cut(s) 252, 551
BplI GAGNNNNNCTC 2 cut(s) 521, 553
BsaAI YACGTR 1 cut(s) 640
BsaHI GRCGYC 2 cut(s) 5, 494
BsaI GGTCTC 1 cut(s) 945
BsaJI CCNNGG 3 cut(s) 141, 356, 714
BsaXI ACNNNNNCTCC 2 cut(s) 545, 575
Bsc4I CCNNNNNNNGG 4 cut(s) 75, 132, 337, 902
Bse3DI GCAATG 1 cut(s) 391
BseBI CCWGG 3 cut(s) 112, 498, 610
BseDI CCNNGG 3 cut(s) 141, 356, 714
BseGI GGATG 4 cut(s) 84, 376, 463, 670
BseLI CCNNNNNNNGG 4 cut(s) 75, 132, 337, 902
BseMI GCAATG 1 cut(s) 391
BseMII CTCAG 3 cut(s) 327, 365, 956
BseRI GAGGAG 3 cut(s) 212, 302, 569
BseX3I CGGCCG 1 cut(s) 48
BseXI GCAGC 1 cut(s) 313
BsgI GTGCAG 1 cut(s) 954
Bsh1236I CGCG 1 cut(s) 975
Bsh1285I CGRYCG 4 cut(s) 51, 285, 528, 778
BshFI GGCC 3 cut(s) 50, 770, 967
BshNI GGYRCC 3 cut(s) 261, 493, 605
BsiEI CGRYCG 4 cut(s) 51, 285, 528, 778
BsiHKCI CYCGRG 1 cut(s) 140
BsiSI CCGG 1 cut(s) 44
BslI CCNNNNNNNGG 4 cut(s) 75, 132, 337, 902
BsmAI GTCTC 2 cut(s) 919, 945
BsmI GAATGC 2 cut(s) 269, 887
BsnI GGCC 3 cut(s) 50, 770, 967
Bso31I GGTCTC 1 cut(s) 945
BsoBI CYCGRG 1 cut(s) 140
Bsp1286I GDGCHC 1 cut(s) 539
Bsp143I GATC 8 cut(s) 16, 31, 102, 136, 157, 282, 987, 1029
Bsp19I CCATGG 1 cut(s) 714
BspACI CCGC 2 cut(s) 47, 69
BspANI GGCC 3 cut(s) 50, 770, 967
BspCNI CTCAG 3 cut(s) 328, 364, 955
BspFNI CGCG 1 cut(s) 975
BspHI TCATGA 1 cut(s) 781
BspLI GGNNCC 6 cut(s) 104, 138, 263, 495, 538, 607
BspMI ACCTGC 1 cut(s) 283
BspPI GGATC 7 cut(s) 26, 97, 110, 131, 144, 165, 995
BspT107I GGYRCC 3 cut(s) 261, 493, 605
BspTNI GGTCTC 1 cut(s) 945
BsrDI GCAATG 1 cut(s) 391
BssECI CCNNGG 3 cut(s) 141, 356, 714
BssMI GATC 8 cut(s) 16, 31, 102, 136, 157, 282, 987, 1029
BssNI GRCGYC 2 cut(s) 5, 494
BssSI CACGAG 2 cut(s) 207, 471
BssT1I CCWWGG 2 cut(s) 356, 714
Bst2BI CACGAG 2 cut(s) 207, 471
Bst2UI CCWGG 3 cut(s) 112, 498, 610
Bst4CI ACNGT 4 cut(s) 266, 779, 897, 939
Bst6I CTCTTC 1 cut(s) 420
BstACI GRCGYC 2 cut(s) 5, 494
BstBAI YACGTR 1 cut(s) 640
BstC8I GCNNGC 4 cut(s) 841, 969, 973, 1026
BstDEI CTNAG 4 cut(s) 336, 351, 707, 942
BstDSI CCRYGG 1 cut(s) 714
BstENI CCTNNNNNAGG 1 cut(s) 335
BstF5I GGATG 4 cut(s) 84, 376, 463, 670
BstFNI CGCG 1 cut(s) 975
BstH2I RGCGCY 1 cut(s) 497
BstHHI GCGC 1 cut(s) 496
BstKTI GATC 8 cut(s) 19, 34, 105, 139, 160, 285, 990, 1032
BstMAI GTCTC 2 cut(s) 919, 945
BstMBI GATC 8 cut(s) 16, 31, 102, 136, 157, 282, 987, 1029
BstMCI CGRYCG 4 cut(s) 51, 285, 528, 778
BstMWI GCNNNNNNNGC 4 cut(s) 361, 797, 882, 977
BstNI CCWGG 3 cut(s) 112, 498, 610
BstSCI CCNGG 3 cut(s) 110, 496, 608
BstSNI TACGTA 1 cut(s) 640
BstUI CGCG 1 cut(s) 975
BstV1I GCAGC 1 cut(s) 313
BstV2I GAAGAC 2 cut(s) 252, 551
BstX2I RGATCY 4 cut(s) 31, 102, 136, 157
BstYI RGATCY 4 cut(s) 31, 102, 136, 157
BstZI CGGCCG 1 cut(s) 48
BsuI GTATCC 1 cut(s) 629
BsuRI GGCC 3 cut(s) 50, 770, 967
BtgI CCRYGG 1 cut(s) 714
BtgZI GCGATG 1 cut(s) 891
BtsCI GGATG 4 cut(s) 84, 376, 463, 670
BveI ACCTGC 1 cut(s) 283
Cac8I GCNNGC 4 cut(s) 841, 969, 973, 1026
CciI TCATGA 1 cut(s) 781
CfoI GCGC 1 cut(s) 496
Cfr13I GGNCC 1 cut(s) 410
CseI GACGC 3 cut(s) 128, 257, 714
Csp6I GTAC 6 cut(s) 165, 641, 677, 910, 946, 995
CviAII CATG 6 cut(s) 99, 240, 516, 566, 715, 782
CviQI GTAC 6 cut(s) 165, 641, 677, 910, 946, 995
DdeI CTNAG 4 cut(s) 336, 351, 707, 942
DinI GGCGCC 1 cut(s) 495
DpnI GATC 8 cut(s) 18, 33, 104, 138, 159, 284, 989, 1031
DpnII GATC 8 cut(s) 16, 31, 102, 136, 157, 282, 987, 1029
EaeI YGGCCR 1 cut(s) 48
EagI CGGCCG 1 cut(s) 48
Eam1104I CTCTTC 1 cut(s) 420
EarI CTCTTC 1 cut(s) 420
EciI GGCGGA 1 cut(s) 58
EclXI CGGCCG 1 cut(s) 48
Eco105I TACGTA 1 cut(s) 640
Eco130I CCWWGG 2 cut(s) 356, 714
Eco24I GRGCYC 1 cut(s) 539
Eco31I GGTCTC 1 cut(s) 945
Eco47I GGWCC 1 cut(s) 410
Eco52I CGGCCG 1 cut(s) 48
Eco88I CYCGRG 1 cut(s) 140
EcoNI CCTNNNNNAGG 1 cut(s) 335
EcoRII CCWGG 3 cut(s) 110, 496, 608
EcoT14I CCWWGG 2 cut(s) 356, 714
EcoT38I GRGCYC 1 cut(s) 539
EgeI GGCGCC 1 cut(s) 495
EheI GGCGCC 1 cut(s) 495
ErhI CCWWGG 2 cut(s) 356, 714
FaeI CATG 6 cut(s) 102, 243, 519, 569, 718, 785
FalI AAGNNNNNCTT 2 cut(s) 315, 347
FatI CATG 6 cut(s) 98, 239, 515, 565, 714, 781
FblI GTMKAC 1 cut(s) 774
Fnu4HI GCNGC 2 cut(s) 48, 327
FokI GGATG 4 cut(s) 91, 383, 470, 657
FriOI GRGCYC 1 cut(s) 539
Fsp4HI GCNGC 2 cut(s) 48, 327
FspBI CTAG 5 cut(s) 549, 585, 690, 813, 825
GlaI GCGC 1 cut(s) 495
GluI GCNGC 2 cut(s) 48, 327
HaeII RGCGCY 1 cut(s) 497
HaeIII GGCC 3 cut(s) 50, 770, 967
HapII CCGG 1 cut(s) 44
HgaI GACGC 3 cut(s) 128, 257, 714
HhaI GCGC 1 cut(s) 496
Hin1I GRCGYC 2 cut(s) 5, 494
Hin1II CATG 6 cut(s) 102, 243, 519, 569, 718, 785
Hin6I GCGC 1 cut(s) 494
HinP1I GCGC 1 cut(s) 494
HincII GTYRAC 2 cut(s) 775, 822
HindII GTYRAC 2 cut(s) 775, 822
HinfI GANTC 4 cut(s) 65, 83, 474, 685
HpaII CCGG 1 cut(s) 44
Hpy166II GTNNAC 3 cut(s) 775, 822, 995
Hpy188I TCNGA 4 cut(s) 96, 337, 424, 1034
Hpy188III TCNNGA 6 cut(s) 62, 140, 408, 782, 922, 1006
Hpy8I GTNNAC 3 cut(s) 775, 822, 995
Hpy99I CGWCG 1 cut(s) 776
HpyAV CCTTC 3 cut(s) 109, 368, 760
HpyCH4III ACNGT 4 cut(s) 266, 779, 897, 939
HpyCH4IV ACGT 2 cut(s) 600, 639
HpyCH4V TGCA 9 cut(s) 153, 206, 269, 384, 395, 620, 800, 955, 971
HpyF10VI GCNNNNNNNGC 4 cut(s) 361, 797, 882, 977
HpyF3I CTNAG 4 cut(s) 336, 351, 707, 942
HpySE526I ACGT 2 cut(s) 600, 639
Hsp92I GRCGYC 2 cut(s) 5, 494
Hsp92II CATG 6 cut(s) 102, 243, 519, 569, 718, 785
HspAI GCGC 1 cut(s) 494
KasI GGCGCC 1 cut(s) 493
Kzo9I GATC 8 cut(s) 16, 31, 102, 136, 157, 282, 987, 1029
LmnI GCTCC 3 cut(s) 289, 542, 914
Lsp1109I GCAGC 1 cut(s) 313
LweI GCATC 3 cut(s) 679, 778, 840
MaeI CTAG 5 cut(s) 549, 585, 690, 813, 825
MaeII ACGT 2 cut(s) 600, 639
MaeIII GTNAC 3 cut(s) 446, 475, 561
MalI GATC 8 cut(s) 18, 33, 104, 138, 159, 284, 989, 1031
MboI GATC 8 cut(s) 16, 31, 102, 136, 157, 282, 987, 1029
MboII GAAGA 8 cut(s) 65, 152, 257, 437, 556, 625, 642, 797
MflI RGATCY 4 cut(s) 31, 102, 136, 157
MhlI GDGCHC 1 cut(s) 539
MluCI AATT 1 cut(s) 303
MluI ACGCGT 1 cut(s) 973
Mly113I GGCGCC 1 cut(s) 494
MlyI GAGTC 1 cut(s) 483
MnlI CCTC 7 cut(s) 136, 190, 252, 280, 331, 547, 570
MroXI GAANNNNTTC 1 cut(s) 57
MseI TTAA 2 cut(s) 317, 441
MslI CAYNNNNRTG 1 cut(s) 786
MspI CCGG 1 cut(s) 44
MspR9I CCNGG 3 cut(s) 112, 498, 610
Mva1269I GAATGC 2 cut(s) 269, 887
MvaI CCWGG 3 cut(s) 112, 498, 610
MvnI CGCG 1 cut(s) 975
MwoI GCNNNNNNNGC 4 cut(s) 361, 797, 882, 977
NarI GGCGCC 1 cut(s) 494
NcoI CCATGG 1 cut(s) 714
NdeII GATC 8 cut(s) 16, 31, 102, 136, 157, 282, 987, 1029
NlaIII CATG 6 cut(s) 102, 243, 519, 569, 718, 785
NlaIV GGNNCC 6 cut(s) 104, 138, 263, 495, 538, 607
NmuCI GTSAC 2 cut(s) 475, 561
PagI TCATGA 1 cut(s) 781
PcsI WCGNNNNNNNCGW 1 cut(s) 227
PctI GAATGC 2 cut(s) 269, 887
PdmI GAANNNNTTC 1 cut(s) 57
PfeI GAWTC 3 cut(s) 65, 83, 685
PkrI GCNGC 2 cut(s) 49, 328
Ple19I CGATCG 1 cut(s) 285
PleI GAGTC 1 cut(s) 482
PluTI GGCGCC 1 cut(s) 497
PpsI GAGTC 1 cut(s) 482
Ppu21I YACGTR 1 cut(s) 640
Psp6I CCWGG 3 cut(s) 110, 496, 608
PspGI CCWGG 3 cut(s) 110, 496, 608
PspN4I GGNNCC 6 cut(s) 104, 138, 263, 495, 538, 607
PspPI GGNCC 1 cut(s) 410
PsuI RGATCY 4 cut(s) 31, 102, 136, 157
PvuI CGATCG 1 cut(s) 285
RsaI GTAC 6 cut(s) 166, 642, 678, 911, 947, 996
RsaNI GTAC 6 cut(s) 165, 641, 677, 910, 946, 995
RseI CAYNNNNRTG 1 cut(s) 786
SalI GTCGAC 1 cut(s) 773
SaqAI TTAA 2 cut(s) 317, 441
SatI GCNGC 2 cut(s) 48, 327
Sau3AI GATC 8 cut(s) 16, 31, 102, 136, 157, 282, 987, 1029
Sau96I GGNCC 1 cut(s) 410
ScaI AGTACT 1 cut(s) 947
SchI GAGTC 1 cut(s) 483
ScrFI CCNGG 3 cut(s) 112, 498, 610
SduI GDGCHC 1 cut(s) 539
SfaNI GCATC 3 cut(s) 679, 778, 840
SfoI GGCGCC 1 cut(s) 495
SinI GGWCC 1 cut(s) 410
SmiMI CAYNNNNRTG 1 cut(s) 786
SnaBI TACGTA 1 cut(s) 640
Sse9I AATT 1 cut(s) 303
SsiI CCGC 2 cut(s) 47, 69
SspDI GGCGCC 1 cut(s) 493
SspMI CTAG 5 cut(s) 549, 585, 690, 813, 825
StyD4I CCNGG 3 cut(s) 110, 496, 608
StyI CCWWGG 2 cut(s) 356, 714
TaaI ACNGT 4 cut(s) 266, 779, 897, 939
TaiI ACGT 2 cut(s) 603, 642
TaqI TCGA 5 cut(s) 221, 285, 524, 627, 774
TaqII GACCGA 1 cut(s) 542
TasI AATT 1 cut(s) 303
TatI WGTACW 2 cut(s) 945, 994
TauI GCSGC 1 cut(s) 50
TfiI GAWTC 3 cut(s) 65, 83, 685
Tru1I TTAA 2 cut(s) 317, 441
Tru9I TTAA 2 cut(s) 317, 441
TseFI GTSAC 2 cut(s) 475, 561
TseI GCWGC 1 cut(s) 326
Tsp45I GTSAC 2 cut(s) 475, 561
TspDTI ATGAA 5 cut(s) 262, 387, 402, 798, 894
TspGWI ACGGA 1 cut(s) 927
VpaK11BI GGWCC 1 cut(s) 410
XagI CCTNNNNNAGG 1 cut(s) 335
XmiI GTMKAC 1 cut(s) 774
XmnI GAANNNNTTC 1 cut(s) 57
XspI CTAG 5 cut(s) 549, 585, 690, 813, 825
ZrmI AGTACT 1 cut(s) 947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.