AT1G27970

nuclear transport factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009057
Physical Location & Seq
Forward (+)
9746709 .. 9748467
1759 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G27970.2

Sequence Viewer

Length: 381 bp
ATGTCTCAGATGGATCCCGACGCTGTATCTAAGGCCTTCGTCGAACATTACTATTCCACCTTCGACACCAACCGTGTCGGTCTCGCTGGTCTCTATCAGGAAGCTTCTATGCTCACCTTCGAAGGTCAAAAGATCCAAGGAGTCCAGAGCATCGTCGCTAAGCTCACCTCTCTTCCTTTCCAGCAGTGCAAGCATCACATCTCCACCGTTGATTGTCAGCCTTCTGGTCCTGCTTCCGGTATGCTCGTTTTCGTCTCCGGTAATCTCCAGCTCGCCGGAGAAGAACACGCTCTCAAGTTCAGCCAGATGTTTCATTTGATGCCGACGCCACAAGGAAGCTTTTACGTGTTCAACGATATATTCAGGTTGAACTATGCCTGA

Protein Analysis

126

Amino Acids

14.0

Weight (kDa)

5.87

Isoelectric Point (pI)

44.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NTF2 PF02136 9 - 122 2.5e-37 Nuclear transport factor 2 (NTF2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 8, 21, 127
AcyI GRCGYC 1 cut(s) 326
AfiI CCNNNNNNNGG 1 cut(s) 236
AflIII ACRYGT 1 cut(s) 345
AgsI TTSAA 2 cut(s) 352, 370
AluBI AGCT 4 cut(s) 104, 163, 271, 339
AluI AGCT 4 cut(s) 104, 163, 271, 339
Alw26I GTCTC 4 cut(s) 9, 86, 95, 259
AlwI GGATC 3 cut(s) 8, 21, 127
AoxI GGCC 1 cut(s) 33
AspS9I GGNCC 1 cut(s) 227
AsuHPI GGTGA 2 cut(s) 106, 157
AsuII TTCGAA 1 cut(s) 120
AvaII GGWCC 1 cut(s) 227
BamHI GGATCC 1 cut(s) 13
BccI CCATC 1 cut(s) 4
BcoDI GTCTC 4 cut(s) 9, 86, 95, 259
BlpI GCTNAGC 1 cut(s) 159
Bme18I GGWCC 1 cut(s) 227
BmgT120I GGNCC 1 cut(s) 227
BmiI GGNNCC 1 cut(s) 15
BmsI GCATC 3 cut(s) 159, 202, 309
BpmI CTGGAG 1 cut(s) 251
Bpu1102I GCTNAGC 1 cut(s) 159
Bpu14I TTCGAA 1 cut(s) 120
BpuEI CTTGAG 1 cut(s) 278
BsaAI YACGTR 1 cut(s) 346
BsaHI GRCGYC 1 cut(s) 326
BsaI GGTCTC 2 cut(s) 86, 95
BsaJI CCNNGG 1 cut(s) 136
BsaWI WCCGGW 2 cut(s) 236, 257
Bsc4I CCNNNNNNNGG 1 cut(s) 236
BseDI CCNNGG 1 cut(s) 136
BseLI CCNNNNNNNGG 1 cut(s) 236
BseMII CTCAG 1 cut(s) 20
BshFI GGCC 1 cut(s) 35
BsiSI CCGG 3 cut(s) 237, 258, 276
BslI CCNNNNNNNGG 1 cut(s) 236
BsmAI GTCTC 4 cut(s) 9, 86, 95, 259
BsmBI CGTCTC 1 cut(s) 259
BsnI GGCC 1 cut(s) 35
Bso31I GGTCTC 2 cut(s) 86, 95
Bsp119I TTCGAA 1 cut(s) 120
Bsp143I GATC 2 cut(s) 13, 132
Bsp1720I GCTNAGC 1 cut(s) 159
BspANI GGCC 1 cut(s) 35
BspCNI CTCAG 1 cut(s) 19
BspLI GGNNCC 1 cut(s) 15
BspPI GGATC 3 cut(s) 8, 21, 127
BspT104I TTCGAA 1 cut(s) 120
BspTNI GGTCTC 2 cut(s) 86, 95
BssECI CCNNGG 1 cut(s) 136
BssMI GATC 2 cut(s) 13, 132
BssNI GRCGYC 1 cut(s) 326
BssT1I CCWWGG 1 cut(s) 136
Bst4CI ACNGT 2 cut(s) 74, 208
Bst6I CTCTTC 1 cut(s) 177
BstACI GRCGYC 1 cut(s) 326
BstBAI YACGTR 1 cut(s) 346
BstBI TTCGAA 1 cut(s) 120
BstC8I GCNNGC 2 cut(s) 191, 273
BstDEI CTNAG 3 cut(s) 6, 30, 159
BstKTI GATC 2 cut(s) 16, 135
BstMAI GTCTC 4 cut(s) 9, 86, 95, 259
BstMBI GATC 2 cut(s) 13, 132
BstMWI GCNNNNNNNGC 1 cut(s) 190
BstX2I RGATCY 2 cut(s) 13, 132
BstYI RGATCY 2 cut(s) 13, 132
BsuRI GGCC 1 cut(s) 35
BtsI GCAGTG 1 cut(s) 191
BtsIMutI CAGTG 1 cut(s) 191
Cac8I GCNNGC 2 cut(s) 191, 273
Cfr13I GGNCC 1 cut(s) 227
CseI GACGC 2 cut(s) 29, 334
CviJI RGCY 7 cut(s) 35, 104, 163, 220, 271, 303, 339
CviKI_1 RGCY 7 cut(s) 35, 104, 163, 220, 271, 303, 339
DdeI CTNAG 3 cut(s) 6, 30, 159
DpnI GATC 2 cut(s) 15, 134
DpnII GATC 2 cut(s) 13, 132
Eam1104I CTCTTC 1 cut(s) 177
EarI CTCTTC 1 cut(s) 177
Eco130I CCWWGG 1 cut(s) 136
Eco147I AGGCCT 1 cut(s) 35
Eco31I GGTCTC 2 cut(s) 86, 95
Eco47I GGWCC 1 cut(s) 227
EcoT14I CCWWGG 1 cut(s) 136
ErhI CCWWGG 1 cut(s) 136
Esp3I CGTCTC 1 cut(s) 259
FaiI YATR 4 cut(s) 110, 242, 359, 375
GsuI CTGGAG 1 cut(s) 251
HaeIII GGCC 1 cut(s) 35
HapII CCGG 3 cut(s) 237, 258, 276
HgaI GACGC 2 cut(s) 29, 334
Hin1I GRCGYC 1 cut(s) 326
HindIII AAGCTT 2 cut(s) 102, 337
HinfI GANTC 1 cut(s) 141
HpaII CCGG 3 cut(s) 237, 258, 276
HphI GGTGA 2 cut(s) 106, 157
Hpy188I TCNGA 1 cut(s) 9
Hpy188III TCNNGA 3 cut(s) 17, 98, 145
Hpy99I CGWCG 4 cut(s) 23, 44, 158, 328
HpyAV CCTTC 5 cut(s) 46, 70, 116, 127, 231
HpyCH4III ACNGT 2 cut(s) 74, 208
HpyCH4IV ACGT 1 cut(s) 345
HpyCH4V TGCA 1 cut(s) 189
HpyF10VI GCNNNNNNNGC 1 cut(s) 190
HpyF3I CTNAG 3 cut(s) 6, 30, 159
HpySE526I ACGT 1 cut(s) 345
Hsp92I GRCGYC 1 cut(s) 326
Kzo9I GATC 2 cut(s) 13, 132
LweI GCATC 3 cut(s) 159, 202, 309
MaeII ACGT 1 cut(s) 345
MalI GATC 2 cut(s) 15, 134
MboI GATC 2 cut(s) 13, 132
MboII GAAGA 2 cut(s) 164, 293
MflI RGATCY 2 cut(s) 13, 132
MlyI GAGTC 1 cut(s) 150
MnlI CCTC 1 cut(s) 178
MspI CCGG 3 cut(s) 237, 258, 276
MwoI GCNNNNNNNGC 1 cut(s) 190
NdeII GATC 2 cut(s) 13, 132
NlaIV GGNNCC 1 cut(s) 15
NspV TTCGAA 1 cut(s) 120
PceI AGGCCT 1 cut(s) 35
PcsI WCGNNNNNNNCGW 1 cut(s) 351
PleI GAGTC 1 cut(s) 149
PpsI GAGTC 1 cut(s) 149
Ppu21I YACGTR 1 cut(s) 346
PspN4I GGNNCC 1 cut(s) 15
PspPI GGNCC 1 cut(s) 227
PsuI RGATCY 2 cut(s) 13, 132
Sau3AI GATC 2 cut(s) 13, 132
Sau96I GGNCC 1 cut(s) 227
SchI GAGTC 1 cut(s) 150
SfaNI GCATC 3 cut(s) 159, 202, 309
SfuI TTCGAA 1 cut(s) 120
SinI GGWCC 1 cut(s) 227
SmlI CTYRAG 1 cut(s) 293
SmoI CTYRAG 1 cut(s) 293
SseBI AGGCCT 1 cut(s) 35
StuI AGGCCT 1 cut(s) 35
StyI CCWWGG 1 cut(s) 136
TaaI ACNGT 2 cut(s) 74, 208
TaiI ACGT 1 cut(s) 348
TaqI TCGA 3 cut(s) 42, 63, 120
TaqII GACCGA 1 cut(s) 68
TscAI CASTG 1 cut(s) 191
TspDTI ATGAA 1 cut(s) 302
TspRI CASTG 1 cut(s) 191
VpaK11BI GGWCC 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.