RchiOBHm_Chr5g0003881

Nuclear transport factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0009057
Physical Location & Seq
Reverse (-)
2442970 .. 2445709
2740 bp
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UTR
Exon/CDS
Intron
PRQ28517

Sequence Viewer

Length: 489 bp
ATGAGTATGCTTTGCACACTGCTATTTCTTCTCTTGTTGAACGCTGTTTTACTACTCATGCTTCTGAGGCGAAGAAGCAGATCAAACAGAGAGGGAGCCGAGCGAAAAGAAATGGAAGAACAAGTCGAAATGGTGGGGAGGGCATTTGTGGATCATTATTACCTTCTTTTCGACAGTGATAGAGCTTCCCTGTCTACTCTATATCAACCAACCTCTATGTTGAGCTTTGAGGGCCAAAAGATTTTTGGGGTTGACGATATTTCTGTGAAGCTTAATCAGTTACCATTCGATCAATGCAAGCACCTGATCAGTACTATCGATTCCCAGCCATCATCGACAACCGGTGGCATTGTGGTCTTTGTCAGCGGCAGCCTCCAATTACCGGGGGAAGAACACCTTCTAAGATTTAGCCAGATGTTCCACTTGATTCCAACTCCACAAGGAAGCTTCTTTGTCCAGAATGATATATTTCGTCTGAATTATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTF2

Protein Analysis

162

Amino Acids

18.47

Weight (kDa)

5.77

Isoelectric Point (pI)

48.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NTF2 PF02136 45 - 159 1.4e-32 Nuclear transport factor 2 (NTF2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 194
AciI CCGC 1 cut(s) 366
AclWI GGATC 1 cut(s) 159
AfaI GTAC 1 cut(s) 313
AfiI CCNNNNNNNGG 1 cut(s) 382
AgeI ACCGGT 1 cut(s) 341
AgsI TTSAA 1 cut(s) 40
AluBI AGCT 4 cut(s) 185, 225, 271, 447
AluI AGCT 4 cut(s) 185, 225, 271, 447
AlwI GGATC 1 cut(s) 159
AoxI GGCC 1 cut(s) 232
ApeKI GCWGC 1 cut(s) 369
AsiGI ACCGGT 1 cut(s) 341
Asp700I GAANNNNTTC 1 cut(s) 396
AspS9I GGNCC 1 cut(s) 232
AsuC2I CCSGG 1 cut(s) 384
BbvI GCAGC 1 cut(s) 381
BccI CCATC 1 cut(s) 337
BclI TGATCA 1 cut(s) 306
BcnI CCSGG 1 cut(s) 384
BisI GCNGC 2 cut(s) 367, 370
BlsI GCNGC 2 cut(s) 368, 371
BmcAI AGTACT 1 cut(s) 313
Bme1390I CCNGG 1 cut(s) 384
BmgT120I GGNCC 1 cut(s) 232
BmiI GGNNCC 1 cut(s) 97
BmrFI CCNGG 1 cut(s) 384
BpuMI CCSGG 1 cut(s) 384
Bsa29I ATCGAT 1 cut(s) 318
BsaJI CCNNGG 1 cut(s) 383
BsaWI WCCGGW 1 cut(s) 341
Bsc4I CCNNNNNNNGG 1 cut(s) 382
Bse118I RCCGGY 1 cut(s) 341
BseCI ATCGAT 1 cut(s) 318
BseDI CCNNGG 1 cut(s) 383
BseLI CCNNNNNNNGG 1 cut(s) 382
BseMII CTCAG 1 cut(s) 56
BseXI GCAGC 1 cut(s) 381
BseYI CCCAGC 1 cut(s) 324
BshFI GGCC 1 cut(s) 234
BshTI ACCGGT 1 cut(s) 341
BshVI ATCGAT 1 cut(s) 318
BsiSI CCGG 2 cut(s) 342, 383
BslI CCNNNNNNNGG 1 cut(s) 382
BsnI GGCC 1 cut(s) 234
Bsp143I GATC 4 cut(s) 80, 151, 289, 306
BspACI CCGC 1 cut(s) 366
BspANI GGCC 1 cut(s) 234
BspCNI CTCAG 1 cut(s) 57
BspDI ATCGAT 1 cut(s) 318
BspLI GGNNCC 1 cut(s) 97
BspPI GGATC 1 cut(s) 159
BsrFI RCCGGY 1 cut(s) 341
BssAI RCCGGY 1 cut(s) 341
BssECI CCNNGG 1 cut(s) 383
BssMI GATC 4 cut(s) 80, 151, 289, 306
Bst4CI ACNGT 1 cut(s) 176
BstC8I GCNNGC 1 cut(s) 299
BstDEI CTNAG 2 cut(s) 65, 401
BstKTI GATC 4 cut(s) 83, 154, 292, 309
BstMBI GATC 4 cut(s) 80, 151, 289, 306
BstMWI GCNNNNNNNGC 2 cut(s) 67, 231
BstSCI CCNGG 1 cut(s) 382
BstV1I GCAGC 1 cut(s) 381
Bsu15I ATCGAT 1 cut(s) 318
BsuRI GGCC 1 cut(s) 234
BsuTUI ATCGAT 1 cut(s) 318
BtsI GCAGTG 1 cut(s) 17
BtsIMutI CAGTG 2 cut(s) 17, 181
Cac8I GCNNGC 1 cut(s) 299
Cfr10I RCCGGY 1 cut(s) 341
Cfr13I GGNCC 1 cut(s) 232
ClaI ATCGAT 1 cut(s) 318
Csp6I GTAC 1 cut(s) 312
CspAI ACCGGT 1 cut(s) 341
CviAII CATG 1 cut(s) 58
CviJI RGCY 9 cut(s) 98, 185, 225, 234, 271, 328, 372, 411, 447
CviKI_1 RGCY 9 cut(s) 98, 185, 225, 234, 271, 328, 372, 411, 447
CviQI GTAC 1 cut(s) 312
DdeI CTNAG 2 cut(s) 65, 401
DpnI GATC 4 cut(s) 82, 153, 291, 308
DpnII GATC 4 cut(s) 80, 151, 289, 306
FaeI CATG 1 cut(s) 61
FaiI YATR 6 cut(s) 8, 59, 202, 218, 467, 483
FalI AAGNNNNNCTT 2 cut(s) 381, 413
FatI CATG 1 cut(s) 57
FbaI TGATCA 1 cut(s) 306
FblI GTMKAC 1 cut(s) 194
Fnu4HI GCNGC 2 cut(s) 367, 370
Fsp4HI GCNGC 2 cut(s) 367, 370
GluI GCNGC 2 cut(s) 367, 370
GsaI CCCAGC 1 cut(s) 328
HaeIII GGCC 1 cut(s) 234
HapII CCGG 2 cut(s) 342, 383
Hin1II CATG 1 cut(s) 61
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HindIII AAGCTT 2 cut(s) 269, 445
HinfI GANTC 2 cut(s) 320, 427
HpaII CCGG 2 cut(s) 342, 383
Hpy166II GTNNAC 2 cut(s) 195, 253
Hpy188I TCNGA 2 cut(s) 66, 477
Hpy188III TCNNGA 1 cut(s) 457
Hpy8I GTNNAC 2 cut(s) 195, 253
HpyAV CCTTC 2 cut(s) 173, 407
HpyCH4III ACNGT 1 cut(s) 176
HpyCH4V TGCA 2 cut(s) 15, 297
HpyF10VI GCNNNNNNNGC 2 cut(s) 67, 231
HpyF3I CTNAG 2 cut(s) 65, 401
Hsp92II CATG 1 cut(s) 61
Ksp22I TGATCA 1 cut(s) 306
Kzo9I GATC 4 cut(s) 80, 151, 289, 306
LmnI GCTCC 1 cut(s) 95
LpnPI CCDG 7 cut(s) 203, 317, 338, 355, 396, 425, 470
Lsp1109I GCAGC 1 cut(s) 381
MaeIII GTNAC 1 cut(s) 279
MalI GATC 4 cut(s) 82, 153, 291, 308
MboI GATC 4 cut(s) 80, 151, 289, 306
MboII GAAGA 4 cut(s) 20, 84, 128, 401
MluCI AATT 2 cut(s) 377, 478
MmeI TCCRAC 1 cut(s) 455
MnlI CCTC 6 cut(s) 60, 85, 132, 223, 223, 383
MroXI GAANNNNTTC 1 cut(s) 396
MseI TTAA 2 cut(s) 273, 487
MspA1I CMGCKG 1 cut(s) 366
MspI CCGG 2 cut(s) 342, 383
MspR9I CCNGG 1 cut(s) 384
MwoI GCNNNNNNNGC 2 cut(s) 67, 231
NciI CCSGG 1 cut(s) 384
NdeII GATC 4 cut(s) 80, 151, 289, 306
NlaIII CATG 1 cut(s) 61
NlaIV GGNNCC 1 cut(s) 97
NmeAIII GCCGAG 1 cut(s) 124
PdmI GAANNNNTTC 1 cut(s) 396
PfeI GAWTC 2 cut(s) 320, 427
PinAI ACCGGT 1 cut(s) 341
PkrI GCNGC 2 cut(s) 368, 371
PspFI CCCAGC 1 cut(s) 324
PspN4I GGNNCC 1 cut(s) 97
PspPI GGNCC 1 cut(s) 232
RsaI GTAC 1 cut(s) 313
RsaNI GTAC 1 cut(s) 312
SaqAI TTAA 2 cut(s) 273, 487
SatI GCNGC 2 cut(s) 367, 370
Sau3AI GATC 4 cut(s) 80, 151, 289, 306
Sau96I GGNCC 1 cut(s) 232
ScaI AGTACT 1 cut(s) 313
ScrFI CCNGG 1 cut(s) 384
SetI ASST 8 cut(s) 165, 187, 215, 227, 273, 306, 399, 449
Sse9I AATT 2 cut(s) 377, 478
SsiI CCGC 1 cut(s) 366
StyD4I CCNGG 1 cut(s) 382
TaaI ACNGT 1 cut(s) 176
TaqI TCGA 5 cut(s) 126, 171, 288, 318, 335
TasI AATT 2 cut(s) 377, 478
TatI WGTACW 1 cut(s) 311
TauI GCSGC 1 cut(s) 369
TfiI GAWTC 2 cut(s) 320, 427
Tru1I TTAA 2 cut(s) 273, 487
Tru9I TTAA 2 cut(s) 273, 487
TscAI CASTG 2 cut(s) 24, 181
TseI GCWGC 1 cut(s) 369
TspRI CASTG 2 cut(s) 24, 181
XcmI CCANNNNNNNNNTGG 1 cut(s) 242
XmiI GTMKAC 1 cut(s) 194
XmnI GAANNNNTTC 1 cut(s) 396
ZrmI AGTACT 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.