MD05G1341700.v1.1

Nuclear transport factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009057
Physical Location & Seq
Forward (+)
46227237 .. 46227605
369 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1341700.v1.1.491

Sequence Viewer

Length: 369 bp
ATGGAAGATCGAAAAATGGAAGAGCAGGTTGCAATGGTGGGGAGGGCATTTGTGGATCATTCCATTTATTTGATACTGATAGAGCTTCCCCTCTCTACCCGTTACCAGCCTACCTCTATGCTGAGCTTTGAGGGGCAGAAGTTATTTGGCGCCGACGATATATCTTCCAAGCTTAAACAGCTGCCGTTTGATCAAAGCAAGCATTTGATCAGCACCATTGATTCCCAGCCATCGTCCCCAACCGGCGGCATTGTGGTTTTCGTCAGCGGTAGCCTCCAGTTACCGGGGGAAGAACACCTCCTGAGATTCAGCCAGGTAATTTATTTATTTAATTTTCTGCAGATAACATGGCGAAATACTAAAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTF2

Protein Analysis

123

Amino Acids

13.89

Weight (kDa)

5.86

Isoelectric Point (pI)

52.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NTF2 PF02136 34 - 109 9.8e-15 Nuclear transport factor 2 (NTF2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 16
AccB1I GGYRCC 1 cut(s) 149
AciI CCGC 2 cut(s) 246, 267
AclWI GGATC 1 cut(s) 63
AcyI GRCGYC 1 cut(s) 150
AfiI CCNNNNNNNGG 2 cut(s) 245, 283
AjnI CCWGG 1 cut(s) 312
AluBI AGCT 4 cut(s) 85, 126, 172, 181
AluI AGCT 4 cut(s) 85, 126, 172, 181
AlwI GGATC 1 cut(s) 63
ApeKI GCWGC 1 cut(s) 181
AspLEI GCGC 1 cut(s) 152
AsuC2I CCSGG 1 cut(s) 285
BanI GGYRCC 1 cut(s) 149
BbvI GCAGC 1 cut(s) 168
BccI CCATC 1 cut(s) 238
BceAI ACGGC 1 cut(s) 169
BciT130I CCWGG 1 cut(s) 314
BclI TGATCA 2 cut(s) 190, 207
BcnI CCSGG 1 cut(s) 285
BfmI CTRYAG 1 cut(s) 338
BfoI RGCGCY 1 cut(s) 153
BfuAI ACCTGC 1 cut(s) 16
BisI GCNGC 2 cut(s) 182, 247
BlpI GCTNAGC 1 cut(s) 122
BlsI GCNGC 2 cut(s) 183, 248
Bme1390I CCNGG 2 cut(s) 285, 314
BmiI GGNNCC 1 cut(s) 151
BmrFI CCNGG 2 cut(s) 285, 314
BpmI CTGGAG 1 cut(s) 260
Bpu1102I GCTNAGC 1 cut(s) 122
BpuMI CCSGG 1 cut(s) 285
BsaHI GRCGYC 1 cut(s) 150
BsaJI CCNNGG 1 cut(s) 284
Bsc4I CCNNNNNNNGG 2 cut(s) 245, 283
Bse118I RCCGGY 1 cut(s) 242
Bse1I ACTGG 1 cut(s) 277
Bse3DI GCAATG 1 cut(s) 39
BseBI CCWGG 1 cut(s) 314
BseDI CCNNGG 1 cut(s) 284
BseLI CCNNNNNNNGG 2 cut(s) 245, 283
BseMI GCAATG 1 cut(s) 39
BseMII CTCAG 2 cut(s) 113, 293
BseNI ACTGG 1 cut(s) 277
BseXI GCAGC 1 cut(s) 168
BseYI CCCAGC 1 cut(s) 225
BshNI GGYRCC 1 cut(s) 149
BsiSI CCGG 2 cut(s) 243, 284
BslFI GGGAC 1 cut(s) 220
BslI CCNNNNNNNGG 2 cut(s) 245, 283
BsmFI GGGAC 1 cut(s) 220
Bsp143I GATC 4 cut(s) 7, 55, 190, 207
Bsp1720I GCTNAGC 1 cut(s) 122
BspACI CCGC 2 cut(s) 246, 267
BspCNI CTCAG 2 cut(s) 114, 294
BspLI GGNNCC 1 cut(s) 151
BspMAI CTGCAG 1 cut(s) 342
BspMI ACCTGC 1 cut(s) 16
BspPI GGATC 1 cut(s) 63
BspQI GCTCTTC 1 cut(s) 15
BspT107I GGYRCC 1 cut(s) 149
BsrDI GCAATG 1 cut(s) 39
BsrFI RCCGGY 1 cut(s) 242
BsrI ACTGG 1 cut(s) 277
BssAI RCCGGY 1 cut(s) 242
BssECI CCNNGG 1 cut(s) 284
BssMI GATC 4 cut(s) 7, 55, 190, 207
BssNI GRCGYC 1 cut(s) 150
Bst2UI CCWGG 1 cut(s) 314
Bst6I CTCTTC 1 cut(s) 15
BstACI GRCGYC 1 cut(s) 150
BstC8I GCNNGC 1 cut(s) 200
BstDEI CTNAG 2 cut(s) 122, 302
BstH2I RGCGCY 1 cut(s) 153
BstHHI GCGC 1 cut(s) 152
BstKTI GATC 4 cut(s) 10, 58, 193, 210
BstMBI GATC 4 cut(s) 7, 55, 190, 207
BstMWI GCNNNNNNNGC 1 cut(s) 178
BstNI CCWGG 1 cut(s) 314
BstSCI CCNGG 2 cut(s) 283, 312
BstSFI CTRYAG 1 cut(s) 338
BstV1I GCAGC 1 cut(s) 168
BveI ACCTGC 1 cut(s) 16
Cac8I GCNNGC 1 cut(s) 200
CfoI GCGC 1 cut(s) 152
Cfr10I RCCGGY 1 cut(s) 242
CviAII CATG 1 cut(s) 348
CviJI RGCY 8 cut(s) 85, 109, 126, 172, 181, 229, 273, 312
CviKI_1 RGCY 8 cut(s) 85, 109, 126, 172, 181, 229, 273, 312
DdeI CTNAG 2 cut(s) 122, 302
DinI GGCGCC 1 cut(s) 151
DpnI GATC 4 cut(s) 9, 57, 192, 209
DpnII GATC 4 cut(s) 7, 55, 190, 207
Eam1104I CTCTTC 1 cut(s) 15
EarI CTCTTC 1 cut(s) 15
EcoRII CCWGG 1 cut(s) 312
EgeI GGCGCC 1 cut(s) 151
EheI GGCGCC 1 cut(s) 151
FaeI CATG 1 cut(s) 351
FaiI YATR 3 cut(s) 119, 161, 349
FaqI GGGAC 1 cut(s) 220
FatI CATG 1 cut(s) 347
FbaI TGATCA 2 cut(s) 190, 207
Fnu4HI GCNGC 2 cut(s) 182, 247
Fsp4HI GCNGC 2 cut(s) 182, 247
GlaI GCGC 1 cut(s) 151
GluI GCNGC 2 cut(s) 182, 247
GsaI CCCAGC 1 cut(s) 229
GsuI CTGGAG 1 cut(s) 260
HaeII RGCGCY 1 cut(s) 153
HapII CCGG 2 cut(s) 243, 284
HhaI GCGC 1 cut(s) 152
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 1 cut(s) 351
Hin6I GCGC 1 cut(s) 150
HinP1I GCGC 1 cut(s) 150
HindIII AAGCTT 1 cut(s) 170
HinfI GANTC 2 cut(s) 221, 306
HpaII CCGG 2 cut(s) 243, 284
Hpy188III TCNNGA 1 cut(s) 301
Hpy99I CGWCG 1 cut(s) 158
HpyCH4V TGCA 2 cut(s) 32, 340
HpyF10VI GCNNNNNNNGC 1 cut(s) 178
HpyF3I CTNAG 2 cut(s) 122, 302
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 1 cut(s) 351
HspAI GCGC 1 cut(s) 150
KasI GGCGCC 1 cut(s) 149
Ksp22I TGATCA 2 cut(s) 190, 207
Kzo9I GATC 4 cut(s) 7, 55, 190, 207
LguI GCTCTTC 1 cut(s) 15
LpnPI CCDG 9 cut(s) 11, 119, 239, 256, 290, 297, 299, 314, 326
Lsp1109I GCAGC 1 cut(s) 168
MaeIII GTNAC 2 cut(s) 101, 279
MalI GATC 4 cut(s) 9, 57, 192, 209
MboI GATC 4 cut(s) 7, 55, 190, 207
MboII GAAGA 4 cut(s) 17, 32, 156, 302
MluCI AATT 2 cut(s) 318, 331
Mly113I GGCGCC 1 cut(s) 150
MnlI CCTC 6 cut(s) 36, 101, 124, 124, 284, 308
MseI TTAA 2 cut(s) 174, 330
MspA1I CMGCKG 2 cut(s) 181, 267
MspI CCGG 2 cut(s) 243, 284
MspR9I CCNGG 2 cut(s) 285, 314
MvaI CCWGG 1 cut(s) 314
MwoI GCNNNNNNNGC 1 cut(s) 178
NarI GGCGCC 1 cut(s) 150
NciI CCSGG 1 cut(s) 285
NdeII GATC 4 cut(s) 7, 55, 190, 207
NlaIII CATG 1 cut(s) 351
NlaIV GGNNCC 1 cut(s) 151
PciSI GCTCTTC 1 cut(s) 15
PfeI GAWTC 2 cut(s) 221, 306
PkrI GCNGC 2 cut(s) 183, 248
PluTI GGCGCC 1 cut(s) 153
Psp6I CCWGG 1 cut(s) 312
PspFI CCCAGC 1 cut(s) 225
PspGI CCWGG 1 cut(s) 312
PspN4I GGNNCC 1 cut(s) 151
PstI CTGCAG 1 cut(s) 342
PvuII CAGCTG 1 cut(s) 181
SapI GCTCTTC 1 cut(s) 15
SaqAI TTAA 2 cut(s) 174, 330
SatI GCNGC 2 cut(s) 182, 247
Sau3AI GATC 4 cut(s) 7, 55, 190, 207
ScrFI CCNGG 2 cut(s) 285, 314
SetI ASST 8 cut(s) 30, 87, 116, 128, 174, 183, 300, 318
SfcI CTRYAG 1 cut(s) 338
SfoI GGCGCC 1 cut(s) 151
Sse9I AATT 2 cut(s) 318, 331
SsiI CCGC 2 cut(s) 246, 267
SspDI GGCGCC 1 cut(s) 149
StyD4I CCNGG 2 cut(s) 283, 312
TaqI TCGA 1 cut(s) 10
TasI AATT 2 cut(s) 318, 331
TauI GCSGC 1 cut(s) 249
TfiI GAWTC 2 cut(s) 221, 306
Tru1I TTAA 2 cut(s) 174, 330
Tru9I TTAA 2 cut(s) 174, 330
TseI GCWGC 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.