AT1G29320

Transducin family protein WD-40 repeat family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007269
Physical Location & Seq
Forward (+)
10255250 .. 10258343
3094 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G29320.2

Sequence Viewer

Length: 1407 bp
ATGCCTCGAGTCATCCCTCCGGAGAATCTCGGTTGCCCACCATTCCGTGCTTTAACTTTTGACTCTCTCGGCTTAATAAAAGTGACTGAAGCTCGTGGTCAAGAGAGAGGGATTCCGACTGTAGTGAACACATGGGGAGAGATGAATGCATCTAGAAGTGTTCTTGCTGCTTCCATTGATGACCGGTTAAGGAATCCGCTTTTGGCTGTTGCAAGAAAAGATGGCAATGTTGAGGTTATTAACCCTTGTAATGGTGATCTTCACTTCTCATACTCTGTATTTGGTGATGATGGTTGTTCCCCTGAGGATAACGAAATTTCTGCCCTGCACTTATTCAGGAAAAAGATAGATGATCAGACAGAAAGATCTTGCACATTGCTTACATGTACGAAAAAGGGAGATGTGAGTCTCAGATCAGTTAAATTTCCAGATGCACATGGTAATTCCACGGATGATGCTTCTCCTAAAACATGGAAAGCATGTGGTTCTGGTGAAATATTGGTTGGTAAAGTGGATGGGAGCGAAAACTTCAGCTTATTTGGAGGGAAACGGGTTGAGGCCAATATATGGGATCTTGAACAATGTACAAAGATCTGGAGCGCGAAATGCCCTCCTAAGAACAATCTTGGAATCTTCACGCCTACCTGGTTCACTAGTGCTACGTTCTTGAGCAAGGATGATCACCGTAAATTTGTCACAGGAACCAAGTCTCACCAGGTCCGTCTTTATGACATTTCTACTCAACGTAGACCGGTCCTGTCCTTTGATTTCCGTGAGACCGCTATTACATCAATTGCGGAAGACCCAGACGGTCATACTATCTATGTCGGGAATGCTTCTGCTGACCTAGCTTCCTTTGATATACGAACAGGAAAGCTGTTGGGAAGCTTTTTAGGAAAATGCTCTGGAAGCATAAGATCTGTGGTTAGACATCCACAGCATCAAGTGATAGCTTCTTGCGGGCTGGACCGATATTTACGTGTTTATGACGTTAAGACACGCCAGCTCATTTCTGCGGTTTTCTTGAAGCAGCATCTTACAGGTCTTGTATTCGACTCGGGCTTTTCTGGAGAAGAGACAGCTGTAGCAAACACAGTGGCTGAGGCTGCCACAGAGGAGAAGATGACGATCATGGACCAGGAAGATGATGAAACAGAGAAGGCTCCTGTGAAAAGAAAGAAGTCAAAGAAAGAGAAACGCAGTAGAGAAATAGTCTTTGAGGGTGAAGACGATGAGGAGAATGAAGACGAGATTGAGAAGGCTCCGGTCAAAACAAAAAAGTCAAAGAAAGAGAAACGCAGTAGAGAAAAAGTCTCTGAAGGTGAAGAGAAAGACGAGCTAAGAAGCAAGAAGACACGAGAGCACAAGAAGAAGACCAAGATAGTGAAACACAATCAAGAAGACTAG

Protein Analysis

468

Amino Acids

52.27

Weight (kDa)

7.56

Isoelectric Point (pI)

44.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 175 - 363 2.4e-08 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 188 - 341 1.3e-11 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 218 - 336 7e-08 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 226 - 343 8.8e-08 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 228 - 338 1.3e-06 WDHD1 first WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 567
AccI GTMKAC 1 cut(s) 748
AccII CGCG 1 cut(s) 602
AccIII TCCGGA 1 cut(s) 19
AciI CCGC 5 cut(s) 197, 780, 797, 960, 1016
AclWI GGATC 1 cut(s) 579
AcsI RAATTY 3 cut(s) 315, 422, 689
AcuI CTGAAG 3 cut(s) 108, 514, 1338
AfaI GTAC 2 cut(s) 388, 586
AfiI CCNNNNNNNGG 2 cut(s) 251, 567
AflIII ACRYGT 2 cut(s) 383, 979
AgeI ACCGGT 2 cut(s) 183, 751
AgsI TTSAA 2 cut(s) 578, 1027
AhlI ACTAGT 1 cut(s) 653
AjnI CCWGG 3 cut(s) 644, 714, 1137
AjuI GAANNNNNNNTTGG 4 cut(s) 185, 217, 486, 518
AluBI AGCT 9 cut(s) 92, 534, 851, 877, 888, 953, 1006, 1082, 1339
AluI AGCT 9 cut(s) 92, 534, 851, 877, 888, 953, 1006, 1082, 1339
Alw21I GWGCWC 1 cut(s) 1365
Alw26I GTCTC 5 cut(s) 413, 714, 770, 1070, 1318
AlwI GGATC 1 cut(s) 579
AlwNI CAGNNNCTG 1 cut(s) 1100
Ama87I CYCGRG 2 cut(s) 6, 1057
Aor13HI TCCGGA 1 cut(s) 19
AoxI GGCC 1 cut(s) 558
ApeKI GCWGC 3 cut(s) 167, 1030, 1106
ApoI RAATTY 3 cut(s) 315, 422, 689
AsiGI ACCGGT 2 cut(s) 183, 751
AspLEI GCGC 1 cut(s) 602
AspS9I GGNCC 4 cut(s) 718, 754, 967, 1135
AsuHPI GGTGA 7 cut(s) 266, 296, 503, 674, 704, 1235, 1334
AvaI CYCGRG 2 cut(s) 6, 1057
AvaII GGWCC 4 cut(s) 718, 754, 967, 1135
AxyI CCTNAGG 1 cut(s) 303
BauI CACGAG 2 cut(s) 93, 1356
BbsI GAAGAC 5 cut(s) 807, 1233, 1251, 1358, 1379
Bbv12I GWGCWC 1 cut(s) 1365
BbvCI CCTCAGC 1 cut(s) 1101
BbvI GCAGC 3 cut(s) 154, 1042, 1093
BccI CCATC 3 cut(s) 215, 284, 509
BcgI CGANNNNNNTGC 2 cut(s) 302, 336
BciT130I CCWGG 3 cut(s) 646, 716, 1139
BclI TGATCA 2 cut(s) 352, 679
BcoDI GTCTC 5 cut(s) 413, 714, 770, 1070, 1318
BcuI ACTAGT 1 cut(s) 653
BfaI CTAG 4 cut(s) 153, 654, 848, 1405
BfmI CTRYAG 2 cut(s) 120, 1083
BglII AGATCT 3 cut(s) 365, 591, 917
BisI GCNGC 3 cut(s) 168, 1031, 1107
BlsI GCNGC 3 cut(s) 169, 1032, 1108
Bme1390I CCNGG 3 cut(s) 646, 716, 1139
Bme18I GGWCC 4 cut(s) 718, 754, 967, 1135
BmeT110I CYCGRG 2 cut(s) 6, 1057
BmgT120I GGNCC 4 cut(s) 718, 754, 967, 1135
BmiI GGNNCC 3 cut(s) 703, 1164, 1263
BmrFI CCNGG 3 cut(s) 646, 716, 1139
BmsI GCATC 5 cut(s) 158, 421, 445, 949, 1042
BpiI GAAGAC 5 cut(s) 807, 1233, 1251, 1358, 1379
BpmI CTGGAG 2 cut(s) 616, 1089
Bpu10I CCTNAGC 1 cut(s) 1101
BpuEI CTTGAG 1 cut(s) 688
BsaAI YACGTR 1 cut(s) 980
BsaBI GATNNNNATC 1 cut(s) 1127
BsaI GGTCTC 1 cut(s) 770
BsaJI CCNNGG 1 cut(s) 447
BsaWI WCCGGW 4 cut(s) 19, 183, 751, 1264
Bsc4I CCNNNNNNNGG 2 cut(s) 251, 567
Bse118I RCCGGY 2 cut(s) 183, 751
Bse21I CCTNAGG 1 cut(s) 303
Bse3DI GCAATG 2 cut(s) 232, 374
Bse8I GATNNNNATC 1 cut(s) 1127
BseAI TCCGGA 1 cut(s) 19
BseBI CCWGG 3 cut(s) 646, 716, 1139
BseDI CCNNGG 1 cut(s) 447
BseGI GGATG 5 cut(s) 12, 457, 520, 682, 931
BseJI GATNNNNATC 1 cut(s) 1127
BseLI CCNNNNNNNGG 2 cut(s) 251, 567
BseMI GCAATG 2 cut(s) 232, 374
BseMII CTCAG 3 cut(s) 294, 424, 1092
BseRI GAGGAG 2 cut(s) 1130, 1250
BseXI GCAGC 3 cut(s) 154, 1042, 1093
BsgI GTGCAG 1 cut(s) 311
Bsh1236I CGCG 1 cut(s) 602
BshFI GGCC 1 cut(s) 560
BshTI ACCGGT 2 cut(s) 183, 751
BsiHKAI GWGCWC 1 cut(s) 1365
BsiHKCI CYCGRG 2 cut(s) 6, 1057
BsiSI CCGG 4 cut(s) 20, 184, 752, 1265
BslI CCNNNNNNNGG 2 cut(s) 251, 567
BsmAI GTCTC 5 cut(s) 413, 714, 770, 1070, 1318
BsmI GAATGC 2 cut(s) 151, 838
BsnI GGCC 1 cut(s) 560
Bso31I GGTCTC 1 cut(s) 770
BsoBI CYCGRG 2 cut(s) 6, 1057
Bsp1286I GDGCHC 1 cut(s) 1365
Bsp13I TCCGGA 1 cut(s) 19
Bsp1407I TGTACA 1 cut(s) 584
Bsp143I GATC 9 cut(s) 256, 352, 365, 413, 571, 591, 679, 917, 1128
BspACI CCGC 5 cut(s) 197, 780, 797, 960, 1016
BspANI GGCC 1 cut(s) 560
BspCNI CTCAG 3 cut(s) 295, 423, 1093
BspEI TCCGGA 1 cut(s) 19
BspFNI CGCG 1 cut(s) 602
BspLI GGNNCC 3 cut(s) 703, 1164, 1263
BspPI GGATC 1 cut(s) 579
BspTNI GGTCTC 1 cut(s) 770
BsrDI GCAATG 2 cut(s) 232, 374
BsrFI RCCGGY 2 cut(s) 183, 751
BsrGI TGTACA 1 cut(s) 584
BssAI RCCGGY 2 cut(s) 183, 751
BssECI CCNNGG 1 cut(s) 447
BssMI GATC 9 cut(s) 256, 352, 365, 413, 571, 591, 679, 917, 1128
BssSI CACGAG 2 cut(s) 93, 1356
Bst2BI CACGAG 2 cut(s) 93, 1356
Bst2UI CCWGG 3 cut(s) 646, 716, 1139
Bst4CI ACNGT 4 cut(s) 121, 686, 812, 1096
Bst6I CTCTTC 2 cut(s) 1068, 1320
BstAUI TGTACA 1 cut(s) 584
BstBAI YACGTR 1 cut(s) 980
BstC8I GCNNGC 2 cut(s) 962, 1004
BstDEI CTNAG 5 cut(s) 303, 410, 615, 1101, 1340
BstDSI CCRYGG 1 cut(s) 447
BstF5I GGATG 5 cut(s) 12, 457, 520, 682, 931
BstFNI CGCG 1 cut(s) 602
BstHHI GCGC 1 cut(s) 602
BstKTI GATC 9 cut(s) 259, 355, 368, 416, 574, 594, 682, 920, 1131
BstMAI GTCTC 5 cut(s) 413, 714, 770, 1070, 1318
BstMBI GATC 9 cut(s) 256, 352, 365, 413, 571, 591, 679, 917, 1128
BstMWI GCNNNNNNNGC 4 cut(s) 606, 848, 909, 1106
BstNI CCWGG 3 cut(s) 646, 716, 1139
BstNSI RCATGY 2 cut(s) 387, 483
BstSCI CCNGG 3 cut(s) 644, 714, 1137
BstSFI CTRYAG 2 cut(s) 120, 1083
BstUI CGCG 1 cut(s) 602
BstV1I GCAGC 3 cut(s) 154, 1042, 1093
BstV2I GAAGAC 5 cut(s) 807, 1233, 1251, 1358, 1379
BstX2I RGATCY 4 cut(s) 365, 571, 591, 917
BstYI RGATCY 4 cut(s) 365, 571, 591, 917
Bsu36I CCTNAGG 1 cut(s) 303
BsuRI GGCC 1 cut(s) 560
BtgI CCRYGG 1 cut(s) 447
BtsCI GGATG 5 cut(s) 12, 457, 520, 682, 931
BtsIMutI CAGTG 1 cut(s) 1101
Cac8I GCNNGC 2 cut(s) 962, 1004
CaiI CAGNNNCTG 1 cut(s) 1100
CfoI GCGC 1 cut(s) 602
Cfr10I RCCGGY 2 cut(s) 183, 751
Cfr13I GGNCC 4 cut(s) 718, 754, 967, 1135
CsiI ACCWGGT 2 cut(s) 644, 714
Csp6I GTAC 2 cut(s) 387, 585
CspAI ACCGGT 2 cut(s) 183, 751
CspCI CAANNNNNGTGG 2 cut(s) 1077, 1112
CviAII CATG 6 cut(s) 132, 384, 437, 471, 480, 1132
CviQI GTAC 2 cut(s) 387, 585
DdeI CTNAG 5 cut(s) 303, 410, 615, 1101, 1340
DpnI GATC 9 cut(s) 258, 354, 367, 415, 573, 593, 681, 919, 1130
DpnII GATC 9 cut(s) 256, 352, 365, 413, 571, 591, 679, 917, 1128
Eam1104I CTCTTC 2 cut(s) 1068, 1320
EarI CTCTTC 2 cut(s) 1068, 1320
Eco31I GGTCTC 1 cut(s) 770
Eco47I GGWCC 4 cut(s) 718, 754, 967, 1135
Eco57I CTGAAG 3 cut(s) 108, 514, 1338
Eco81I CCTNAGG 1 cut(s) 303
Eco88I CYCGRG 2 cut(s) 6, 1057
EcoRII CCWGG 3 cut(s) 644, 714, 1137
EcoT22I ATGCAT 1 cut(s) 151
FaeI CATG 6 cut(s) 135, 387, 440, 474, 483, 1135
FatI CATG 6 cut(s) 131, 383, 436, 470, 479, 1131
FauI CCCGC 1 cut(s) 953
FbaI TGATCA 2 cut(s) 352, 679
FblI GTMKAC 1 cut(s) 748
Fnu4HI GCNGC 3 cut(s) 168, 1031, 1107
FokI GGATG 4 cut(s) 464, 527, 689, 918
Fsp4HI GCNGC 3 cut(s) 168, 1031, 1107
FspBI CTAG 4 cut(s) 153, 654, 848, 1405
GlaI GCGC 1 cut(s) 601
GluI GCNGC 3 cut(s) 168, 1031, 1107
GsuI CTGGAG 2 cut(s) 616, 1089
HaeIII GGCC 1 cut(s) 560
HapII CCGG 4 cut(s) 20, 184, 752, 1265
HhaI GCGC 1 cut(s) 602
Hin1II CATG 6 cut(s) 135, 387, 440, 474, 483, 1135
Hin6I GCGC 1 cut(s) 600
HinP1I GCGC 1 cut(s) 600
HindIII AAGCTT 1 cut(s) 886
HinfI GANTC 8 cut(s) 9, 25, 62, 112, 193, 406, 630, 1055
HpaII CCGG 4 cut(s) 20, 184, 752, 1265
HphI GGTGA 7 cut(s) 266, 296, 503, 674, 704, 1235, 1334
Hpy166II GTNNAC 3 cut(s) 127, 651, 749
Hpy188I TCNGA 4 cut(s) 117, 357, 413, 1318
Hpy8I GTNNAC 3 cut(s) 127, 651, 749
HpyAV CCTTC 3 cut(s) 1153, 1252, 1313
HpyCH4III ACNGT 4 cut(s) 121, 686, 812, 1096
HpyCH4IV ACGT 4 cut(s) 662, 745, 979, 990
HpyCH4V TGCA 5 cut(s) 149, 212, 328, 372, 434
HpyF10VI GCNNNNNNNGC 4 cut(s) 606, 848, 909, 1106
HpyF3I CTNAG 5 cut(s) 303, 410, 615, 1101, 1340
HpySE526I ACGT 4 cut(s) 662, 745, 979, 990
Hsp92II CATG 6 cut(s) 135, 387, 440, 474, 483, 1135
HspAI GCGC 1 cut(s) 600
Kpn2I TCCGGA 1 cut(s) 19
Ksp22I TGATCA 2 cut(s) 352, 679
Kzo9I GATC 9 cut(s) 256, 352, 365, 413, 571, 591, 679, 917, 1128
LmnI GCTCC 4 cut(s) 519, 597, 1168, 1267
Lsp1109I GCAGC 3 cut(s) 154, 1042, 1093
LweI GCATC 5 cut(s) 158, 421, 445, 949, 1042
MabI ACCWGGT 2 cut(s) 644, 714
MaeI CTAG 4 cut(s) 153, 654, 848, 1405
MaeII ACGT 4 cut(s) 662, 745, 979, 990
MaeIII GTNAC 2 cut(s) 82, 694
MalI GATC 9 cut(s) 258, 354, 367, 415, 573, 593, 681, 919, 1130
MboI GATC 9 cut(s) 256, 352, 365, 413, 571, 591, 679, 917, 1128
MfeI CAATTG 1 cut(s) 792
MflI RGATCY 4 cut(s) 365, 571, 591, 917
MhlI GDGCHC 1 cut(s) 1365
MluCI AATT 5 cut(s) 315, 422, 442, 689, 792
MlyI GAGTC 4 cut(s) 18, 56, 415, 1049
MmeI TCCRAC 1 cut(s) 140
Mph1103I ATGCAT 1 cut(s) 151
MroI TCCGGA 1 cut(s) 19
MseI TTAA 6 cut(s) 53, 74, 188, 240, 420, 993
MspA1I CMGCKG 1 cut(s) 1082
MspI CCGG 4 cut(s) 20, 184, 752, 1265
MspR9I CCNGG 3 cut(s) 646, 716, 1139
MunI CAATTG 1 cut(s) 792
Mva1269I GAATGC 2 cut(s) 151, 838
MvaI CCWGG 3 cut(s) 646, 716, 1139
MvnI CGCG 1 cut(s) 602
MwoI GCNNNNNNNGC 4 cut(s) 606, 848, 909, 1106
NdeII GATC 9 cut(s) 256, 352, 365, 413, 571, 591, 679, 917, 1128
NlaIII CATG 6 cut(s) 135, 387, 440, 474, 483, 1135
NlaIV GGNNCC 3 cut(s) 703, 1164, 1263
NmeAIII GCCGAG 1 cut(s) 48
NmuCI GTSAC 2 cut(s) 82, 694
NsiI ATGCAT 1 cut(s) 151
NspI RCATGY 2 cut(s) 387, 483
PaeR7I CTCGAG 1 cut(s) 6
PciI ACATGT 1 cut(s) 383
PctI GAATGC 2 cut(s) 151, 838
PfeI GAWTC 4 cut(s) 25, 112, 193, 630
PflMI CCANNNNNTGG 1 cut(s) 567
PinAI ACCGGT 2 cut(s) 183, 751
PkrI GCNGC 3 cut(s) 169, 1032, 1108
PleI GAGTC 4 cut(s) 17, 56, 414, 1049
PpsI GAGTC 4 cut(s) 17, 56, 414, 1049
Ppu21I YACGTR 1 cut(s) 980
PscI ACATGT 1 cut(s) 383
Psp6I CCWGG 3 cut(s) 644, 714, 1137
PspGI CCWGG 3 cut(s) 644, 714, 1137
PspN4I GGNNCC 3 cut(s) 703, 1164, 1263
PspPI GGNCC 4 cut(s) 718, 754, 967, 1135
PspXI VCTCGAGB 1 cut(s) 6
PstNI CAGNNNCTG 1 cut(s) 1100
PsuI RGATCY 4 cut(s) 365, 571, 591, 917
PvuII CAGCTG 1 cut(s) 1082
RsaI GTAC 2 cut(s) 388, 586
RsaNI GTAC 2 cut(s) 387, 585
SaqAI TTAA 6 cut(s) 53, 74, 188, 240, 420, 993
SatI GCNGC 3 cut(s) 168, 1031, 1107
Sau3AI GATC 9 cut(s) 256, 352, 365, 413, 571, 591, 679, 917, 1128
Sau96I GGNCC 4 cut(s) 718, 754, 967, 1135
SchI GAGTC 4 cut(s) 18, 56, 415, 1049
ScrFI CCNGG 3 cut(s) 646, 716, 1139
SduI GDGCHC 1 cut(s) 1365
SexAI ACCWGGT 2 cut(s) 644, 714
SfaNI GCATC 5 cut(s) 158, 421, 445, 949, 1042
SfcI CTRYAG 2 cut(s) 120, 1083
Sfr274I CTCGAG 1 cut(s) 6
SinI GGWCC 4 cut(s) 718, 754, 967, 1135
SlaI CTCGAG 1 cut(s) 6
SmlI CTYRAG 2 cut(s) 6, 667
SmoI CTYRAG 2 cut(s) 6, 667
SpeI ACTAGT 1 cut(s) 653
Sse9I AATT 5 cut(s) 315, 422, 442, 689, 792
SsiI CCGC 5 cut(s) 197, 780, 797, 960, 1016
SspI AATATT 1 cut(s) 498
SspMI CTAG 4 cut(s) 153, 654, 848, 1405
StyD4I CCNGG 3 cut(s) 644, 714, 1137
TaaI ACNGT 4 cut(s) 121, 686, 812, 1096
TaiI ACGT 4 cut(s) 665, 748, 982, 993
TaqI TCGA 2 cut(s) 7, 1053
TaqII GACCGA 1 cut(s) 984
TasI AATT 5 cut(s) 315, 422, 442, 689, 792
TatI WGTACW 1 cut(s) 584
TfiI GAWTC 4 cut(s) 25, 112, 193, 630
Tru1I TTAA 6 cut(s) 53, 74, 188, 240, 420, 993
Tru9I TTAA 6 cut(s) 53, 74, 188, 240, 420, 993
TscAI CASTG 1 cut(s) 1101
TseFI GTSAC 2 cut(s) 82, 694
TseI GCWGC 3 cut(s) 167, 1030, 1106
Tsp45I GTSAC 2 cut(s) 82, 694
TspDTI ATGAA 3 cut(s) 158, 1164, 1257
TspGWI ACGGA 4 cut(s) 35, 464, 710, 761
TspRI CASTG 1 cut(s) 1101
Van91I CCANNNNNTGG 1 cut(s) 567
VpaK11BI GGWCC 4 cut(s) 718, 754, 967, 1135
XapI RAATTY 3 cut(s) 315, 422, 689
XbaI TCTAGA 1 cut(s) 152
XceI RCATGY 2 cut(s) 387, 483
XhoI CTCGAG 1 cut(s) 6
XmiI GTMKAC 1 cut(s) 748
XspI CTAG 4 cut(s) 153, 654, 848, 1405
Zsp2I ATGCAT 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.