MD14G1161100.v1.1

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007269
Physical Location & Seq
Reverse (-)
25546472 .. 25550520
4049 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1161100.v1.1.491

Sequence Viewer

Length: 1404 bp
ATGCCTCGTACGACCACTCTCGAGTGCCCAGGTTGCCCTCCACTTCGAGCTCTAACCTTCGACGAGCTTGGTCTCATCAAAGTTATTGAAGCTCGGGGTCAGCAAGGAAGAGCAGAACCTAAGGTGGTTGAGAGATGGGGCGAGCCCGATTCGTCTAAAAGCGTGCTCGCCGCCTCTATCGATGACCGAAAATCCGACCCGTTACTAGCTGTTGCCAGAAGAAATGGGACGATTGAGGTTCTAAATCCTCTTAATGGTGGTCTCCGCTTCGCAGTCACCAATTCTAGTGATAACGGTGGGGACGACCCTGTTGCTGGATTACATTTGTTTGCCAAAAAGAATTTGGAATTGGCTTCTAGGTCTTGCACTTTGCTTACATGTACAACAAATGGAAATGCAAACATAAAATCCTTTGAATTGACTAAACCAGCAGCAGATGTTACTTCTTCTGGGTCTTCAAGAACATGGAGTGTGGGTGCCGGTGGCAGTATCTCATGTTCTAAAGTGGATGGGAGTGAAAACTATTTCTTATTTGGAGGGAAGCGTGTTGAGATGAATGTCTGGGATCTTGACAGCTGTGCCAAGATTTGGACAGCCAAACCTCCTCCTAAAAACAGCCTTGGCATCTTTACGCCAACTTGGTTCACATCTGCCACATTTTTGAGTAAAGATGATCATCGTAAATTTGTTGCTGGCACCAACAGTCATGAGGTTCGTCTCTATGACATTTCTGCTCAGCGAAGACCTGTCATATCATTTGATTTTCAGGAGACTGCTATTAAATCTGTAGCGGAAGATATAGATGGCTACACAATCTATGTAGGGAATGGATCTGGCGACCTAGCTTCTTTTGACATACGCACAGGGAAATTGTTAGGATGCTTTTTGGGCAAGTGTTCAGGTAGTATAAGATCCATTGCAAGGCACCCCGACTTTCCAGTGATAGCATCATGTGGATTGGATAGCTACCTACGCCTTTGGAATGTGAAGACGCGGCAATTACTATCTGCGGTTTACCTGAAGCAGCACTTGTCAAACGTTCTTTTTGACTCCAATTTTGCCGATGAAGAAGCAGAAATTGTCAATCCCCCAACCGATCGGCTACAGCATGATGAGCTTAGTGTTGCTGAGAGAGATGAGGGGGAAACACGACCTGTGAAAAAGAAGAAATCTAAGGAAAAGAATGGAAGCAAGAAGGAAGCCAAAGCAAAAGGCAGAAACAAGGAAGAAGTATCCAGTTTGGGTAGGAGAGATGACGGTGAAACACCACCTGTGAAAAGGCAGAAGTCCAAGGAAAATAAAGGAAGCGAGAAGGAAGCTTCTGAAGAGAGTGGATGCAAGAAGTTGAAATCGAAAAAAAGTAGCAAAAGGCTAAAACGTGAAGATGTTGATAGCACATCGTGA

Protein Analysis

468

Amino Acids

51.08

Weight (kDa)

8.97

Isoelectric Point (pI)

42.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR36-Utp21_1st PF25171 174 - 328 3.1e-06 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st PF25173 185 - 339 1.2e-08 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 224 - 347 4.9e-08 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 224 - 350 5.9e-06 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 476, 695, 924
AccB7I CCANNNNNTGG 1 cut(s) 588
AccII CGCG 1 cut(s) 994
AciI CCGC 5 cut(s) 171, 265, 791, 994, 1010
AclI AACGTT 1 cut(s) 1038
AclWI GGATC 3 cut(s) 573, 838, 906
AcsI RAATTY 2 cut(s) 340, 683
AcuI CTGAAG 2 cut(s) 1040, 1344
AdeI CACNNNGTG 1 cut(s) 1401
AfaI GTAC 2 cut(s) 10, 382
AfiI CCNNNNNNNGG 4 cut(s) 254, 314, 588, 921
AflIII ACRYGT 1 cut(s) 377
AgsI TTSAA 4 cut(s) 89, 416, 459, 1348
AjnI CCWGG 1 cut(s) 28
AjuI GAANNNNNNNTTGG 2 cut(s) 332, 364
AluBI AGCT 9 cut(s) 50, 67, 92, 209, 576, 845, 966, 1117, 1319
AluI AGCT 9 cut(s) 50, 67, 92, 209, 576, 845, 966, 1117, 1319
Alw21I GWGCWC 2 cut(s) 52, 168
Alw26I GTCTC 4 cut(s) 77, 266, 722, 764
AlwI GGATC 3 cut(s) 573, 838, 906
Ama87I CYCGRG 2 cut(s) 20, 93
ApeKI GCWGC 2 cut(s) 431, 1024
ApoI RAATTY 2 cut(s) 340, 683
ArsI GACNNNNNNTTYG 2 cut(s) 1040, 1072
AsuHPI GGTGA 2 cut(s) 268, 1271
AvaI CYCGRG 2 cut(s) 20, 93
AxyI CCTNAGG 1 cut(s) 120
BaeGI GKGCMC 1 cut(s) 29
BanI GGYRCC 3 cut(s) 476, 695, 924
BanII GRGCYC 2 cut(s) 52, 147
BbsI GAAGAC 3 cut(s) 447, 748, 995
Bbv12I GWGCWC 2 cut(s) 52, 168
BbvI GCAGC 2 cut(s) 443, 1036
BccI CCATC 3 cut(s) 129, 503, 797
BcgI CGANNNNNNTGC 2 cut(s) 293, 327
BciT130I CCWGG 1 cut(s) 30
BciVI GTATCC 1 cut(s) 1243
BclI TGATCA 1 cut(s) 673
BcoDI GTCTC 4 cut(s) 77, 266, 722, 764
BfaI CTAG 4 cut(s) 206, 285, 357, 842
BfmI CTRYAG 2 cut(s) 786, 1103
BfuI GTATCC 1 cut(s) 1243
BisI GCNGC 4 cut(s) 171, 432, 995, 1025
BlpI GCTNAGC 1 cut(s) 735
BlsI GCNGC 4 cut(s) 172, 433, 996, 1026
Bme1390I CCNGG 1 cut(s) 30
BmeT110I CYCGRG 2 cut(s) 20, 93
BmiI GGNNCC 3 cut(s) 478, 697, 926
BmrFI CCNGG 1 cut(s) 30
BmsI GCATC 4 cut(s) 633, 869, 956, 1325
BpiI GAAGAC 3 cut(s) 447, 748, 995
Bpu1102I GCTNAGC 1 cut(s) 735
Bsa29I ATCGAT 1 cut(s) 180
BsaBI GATNNNNATC 1 cut(s) 675
BsaI GGTCTC 2 cut(s) 77, 266
BsaJI CCNNGG 3 cut(s) 28, 619, 1290
BsaXI ACNNNNNCTCC 2 cut(s) 460, 490
Bsc4I CCNNNNNNNGG 4 cut(s) 254, 314, 588, 921
Bse118I RCCGGY 1 cut(s) 479
Bse1I ACTGG 2 cut(s) 938, 1236
Bse21I CCTNAGG 1 cut(s) 120
Bse3DI GCAATG 1 cut(s) 915
Bse8I GATNNNNATC 1 cut(s) 675
BseBI CCWGG 1 cut(s) 30
BseCI ATCGAT 1 cut(s) 180
BseDI CCNNGG 3 cut(s) 28, 619, 1290
BseGI GGATG 3 cut(s) 514, 884, 1340
BseJI GATNNNNATC 1 cut(s) 675
BseLI CCNNNNNNNGG 4 cut(s) 254, 314, 588, 921
BseMI GCAATG 1 cut(s) 915
BseMII CTCAG 2 cut(s) 749, 1119
BseNI ACTGG 2 cut(s) 938, 1236
BseRI GAGGAG 1 cut(s) 594
BseSI GKGCMC 1 cut(s) 29
BseXI GCAGC 2 cut(s) 443, 1036
Bsh1236I CGCG 1 cut(s) 994
Bsh1285I CGRYCG 1 cut(s) 1099
BshNI GGYRCC 3 cut(s) 476, 695, 924
BshVI ATCGAT 1 cut(s) 180
BsiEI CGRYCG 1 cut(s) 1099
BsiHKAI GWGCWC 2 cut(s) 52, 168
BsiHKCI CYCGRG 2 cut(s) 20, 93
BsiSI CCGG 1 cut(s) 480
BsiWI CGTACG 1 cut(s) 8
BslFI GGGAC 2 cut(s) 241, 314
BslI CCNNNNNNNGG 4 cut(s) 254, 314, 588, 921
BsmAI GTCTC 4 cut(s) 77, 266, 722, 764
BsmBI CGTCTC 1 cut(s) 722
BsmFI GGGAC 2 cut(s) 241, 314
Bso31I GGTCTC 2 cut(s) 77, 266
BsoBI CYCGRG 2 cut(s) 20, 93
Bsp1286I GDGCHC 4 cut(s) 29, 52, 147, 168
Bsp1407I TGTACA 1 cut(s) 380
Bsp143I GATC 5 cut(s) 565, 673, 830, 911, 1096
Bsp1720I GCTNAGC 1 cut(s) 735
BspACI CCGC 5 cut(s) 171, 265, 791, 994, 1010
BspCNI CTCAG 2 cut(s) 748, 1120
BspDI ATCGAT 1 cut(s) 180
BspFNI CGCG 1 cut(s) 994
BspHI TCATGA 1 cut(s) 706
BspLI GGNNCC 3 cut(s) 478, 697, 926
BspPI GGATC 3 cut(s) 573, 838, 906
BspQI GCTCTTC 1 cut(s) 103
BspT107I GGYRCC 3 cut(s) 476, 695, 924
BspTNI GGTCTC 2 cut(s) 77, 266
BsrDI GCAATG 1 cut(s) 915
BsrFI RCCGGY 1 cut(s) 479
BsrGI TGTACA 1 cut(s) 380
BsrI ACTGG 2 cut(s) 938, 1236
BssAI RCCGGY 1 cut(s) 479
BssECI CCNNGG 3 cut(s) 28, 619, 1290
BssMI GATC 5 cut(s) 565, 673, 830, 911, 1096
BssT1I CCWWGG 2 cut(s) 619, 1290
Bst2UI CCWGG 1 cut(s) 30
Bst4CI ACNGT 3 cut(s) 296, 704, 1259
Bst6I CTCTTC 2 cut(s) 103, 1320
BstAUI TGTACA 1 cut(s) 380
BstC8I GCNNGC 4 cut(s) 143, 164, 168, 694
BstDEI CTNAG 5 cut(s) 120, 735, 1118, 1128, 1173
BstF5I GGATG 3 cut(s) 514, 884, 1340
BstFNI CGCG 1 cut(s) 994
BstKTI GATC 5 cut(s) 568, 676, 833, 914, 1099
BstMAI GTCTC 4 cut(s) 77, 266, 722, 764
BstMBI GATC 5 cut(s) 565, 673, 830, 911, 1096
BstMCI CGRYCG 1 cut(s) 1099
BstMWI GCNNNNNNNGC 4 cut(s) 33, 888, 972, 1114
BstNI CCWGG 1 cut(s) 30
BstNSI RCATGY 1 cut(s) 381
BstSCI CCNGG 1 cut(s) 28
BstSFI CTRYAG 2 cut(s) 786, 1103
BstSLI GKGCMC 1 cut(s) 29
BstUI CGCG 1 cut(s) 994
BstV1I GCAGC 2 cut(s) 443, 1036
BstV2I GAAGAC 3 cut(s) 447, 748, 995
BstX2I RGATCY 3 cut(s) 565, 830, 911
BstYI RGATCY 3 cut(s) 565, 830, 911
Bsu15I ATCGAT 1 cut(s) 180
Bsu36I CCTNAGG 1 cut(s) 120
BsuI GTATCC 1 cut(s) 1243
BsuTUI ATCGAT 1 cut(s) 180
BtsCI GGATG 3 cut(s) 514, 884, 1340
BtsIMutI CAGTG 1 cut(s) 945
Cac8I GCNNGC 4 cut(s) 143, 164, 168, 694
CciI TCATGA 1 cut(s) 706
Cfr10I RCCGGY 1 cut(s) 479
ClaI ATCGAT 1 cut(s) 180
CseI GACGC 1 cut(s) 1000
Csp6I GTAC 2 cut(s) 9, 381
CviAII CATG 6 cut(s) 378, 465, 495, 707, 951, 1109
CviQI GTAC 2 cut(s) 9, 381
DdeI CTNAG 5 cut(s) 120, 735, 1118, 1128, 1173
DpnI GATC 5 cut(s) 567, 675, 832, 913, 1098
DpnII GATC 5 cut(s) 565, 673, 830, 911, 1096
DraIII CACNNNGTG 1 cut(s) 1401
Eam1104I CTCTTC 2 cut(s) 103, 1320
EarI CTCTTC 2 cut(s) 103, 1320
Ecl136II GAGCTC 1 cut(s) 50
Eco130I CCWWGG 2 cut(s) 619, 1290
Eco24I GRGCYC 2 cut(s) 52, 147
Eco31I GGTCTC 2 cut(s) 77, 266
Eco53kI GAGCTC 1 cut(s) 50
Eco57I CTGAAG 2 cut(s) 1040, 1344
Eco81I CCTNAGG 1 cut(s) 120
Eco88I CYCGRG 2 cut(s) 20, 93
EcoICRI GAGCTC 1 cut(s) 50
EcoRII CCWGG 1 cut(s) 28
EcoT14I CCWWGG 2 cut(s) 619, 1290
EcoT38I GRGCYC 2 cut(s) 52, 147
ErhI CCWWGG 2 cut(s) 619, 1290
Esp3I CGTCTC 1 cut(s) 722
FaeI CATG 6 cut(s) 381, 468, 498, 710, 954, 1112
FalI AAGNNNNNCTT 2 cut(s) 1013, 1045
FaqI GGGAC 2 cut(s) 241, 314
FatI CATG 6 cut(s) 377, 464, 494, 706, 950, 1108
FbaI TGATCA 1 cut(s) 673
Fnu4HI GCNGC 4 cut(s) 171, 432, 995, 1025
FokI GGATG 3 cut(s) 521, 891, 1347
FriOI GRGCYC 2 cut(s) 52, 147
Fsp4HI GCNGC 4 cut(s) 171, 432, 995, 1025
FspBI CTAG 4 cut(s) 206, 285, 357, 842
GluI GCNGC 4 cut(s) 171, 432, 995, 1025
HapII CCGG 1 cut(s) 480
HgaI GACGC 1 cut(s) 1000
Hin1II CATG 6 cut(s) 381, 468, 498, 710, 954, 1112
HindIII AAGCTT 1 cut(s) 1317
HinfI GANTC 2 cut(s) 149, 1049
HpaII CCGG 1 cut(s) 480
HphI GGTGA 2 cut(s) 268, 1271
Hpy166II GTNNAC 2 cut(s) 645, 1015
Hpy188I TCNGA 2 cut(s) 196, 1324
Hpy188III TCNNGA 6 cut(s) 20, 459, 569, 707, 767, 1401
Hpy8I GTNNAC 2 cut(s) 645, 1015
Hpy99I CGWCG 1 cut(s) 65
HpyAV CCTTC 3 cut(s) 67, 1189, 1306
HpyCH4III ACNGT 3 cut(s) 296, 704, 1259
HpyCH4IV ACGT 2 cut(s) 1038, 1378
HpyCH4V TGCA 4 cut(s) 366, 398, 920, 1338
HpyF10VI GCNNNNNNNGC 4 cut(s) 33, 888, 972, 1114
HpyF3I CTNAG 5 cut(s) 120, 735, 1118, 1128, 1173
HpySE526I ACGT 2 cut(s) 1038, 1378
Hsp92II CATG 6 cut(s) 381, 468, 498, 710, 954, 1112
Ksp22I TGATCA 1 cut(s) 673
Kzo9I GATC 5 cut(s) 565, 673, 830, 911, 1096
LguI GCTCTTC 1 cut(s) 103
Lsp1109I GCAGC 2 cut(s) 443, 1036
LweI GCATC 4 cut(s) 633, 869, 956, 1325
MaeI CTAG 4 cut(s) 206, 285, 357, 842
MaeII ACGT 2 cut(s) 1038, 1378
MaeIII GTNAC 3 cut(s) 201, 274, 439
MalI GATC 5 cut(s) 567, 675, 832, 913, 1098
MboI GATC 5 cut(s) 565, 673, 830, 911, 1096
MflI RGATCY 3 cut(s) 565, 830, 911
MhlI GDGCHC 4 cut(s) 29, 52, 147, 168
MluCI AATT 9 cut(s) 280, 340, 347, 416, 683, 869, 998, 1054, 1077
MlyI GAGTC 1 cut(s) 1043
MmeI TCCRAC 1 cut(s) 219
MseI TTAA 2 cut(s) 252, 780
MspA1I CMGCKG 1 cut(s) 576
MspI CCGG 1 cut(s) 480
MspR9I CCNGG 1 cut(s) 30
MvaI CCWGG 1 cut(s) 30
MvnI CGCG 1 cut(s) 994
MwoI GCNNNNNNNGC 4 cut(s) 33, 888, 972, 1114
NdeII GATC 5 cut(s) 565, 673, 830, 911, 1096
NlaIII CATG 6 cut(s) 381, 468, 498, 710, 954, 1112
NlaIV GGNNCC 3 cut(s) 478, 697, 926
NmuCI GTSAC 1 cut(s) 274
NspI RCATGY 1 cut(s) 381
PaeR7I CTCGAG 1 cut(s) 20
PagI TCATGA 1 cut(s) 706
PciI ACATGT 1 cut(s) 377
PciSI GCTCTTC 1 cut(s) 103
PcsI WCGNNNNNNNCGW 1 cut(s) 300
PfeI GAWTC 1 cut(s) 149
Pfl23II CGTACG 1 cut(s) 8
PflMI CCANNNNNTGG 1 cut(s) 588
PkrI GCNGC 4 cut(s) 172, 433, 996, 1026
Ple19I CGATCG 1 cut(s) 1099
PleI GAGTC 1 cut(s) 1043
PpsI GAGTC 1 cut(s) 1043
PscI ACATGT 1 cut(s) 377
Psp124BI GAGCTC 1 cut(s) 52
Psp1406I AACGTT 1 cut(s) 1038
Psp6I CCWGG 1 cut(s) 28
PspGI CCWGG 1 cut(s) 28
PspLI CGTACG 1 cut(s) 8
PspN4I GGNNCC 3 cut(s) 478, 697, 926
PsrI GAACNNNNNNTAC 2 cut(s) 481, 513
PsuI RGATCY 3 cut(s) 565, 830, 911
PvuI CGATCG 1 cut(s) 1099
PvuII CAGCTG 1 cut(s) 576
RsaI GTAC 2 cut(s) 10, 382
RsaNI GTAC 2 cut(s) 9, 381
SacI GAGCTC 1 cut(s) 52
SapI GCTCTTC 1 cut(s) 103
SaqAI TTAA 2 cut(s) 252, 780
SatI GCNGC 4 cut(s) 171, 432, 995, 1025
Sau3AI GATC 5 cut(s) 565, 673, 830, 911, 1096
SchI GAGTC 1 cut(s) 1043
ScrFI CCNGG 1 cut(s) 30
SduI GDGCHC 4 cut(s) 29, 52, 147, 168
SfaNI GCATC 4 cut(s) 633, 869, 956, 1325
SfcI CTRYAG 2 cut(s) 786, 1103
Sfr274I CTCGAG 1 cut(s) 20
SlaI CTCGAG 1 cut(s) 20
SmlI CTYRAG 1 cut(s) 20
SmoI CTYRAG 1 cut(s) 20
Sse9I AATT 9 cut(s) 280, 340, 347, 416, 683, 869, 998, 1054, 1077
SsiI CCGC 5 cut(s) 171, 265, 791, 994, 1010
SspMI CTAG 4 cut(s) 206, 285, 357, 842
SstI GAGCTC 1 cut(s) 52
StyD4I CCNGG 1 cut(s) 28
StyI CCWWGG 2 cut(s) 619, 1290
TaaI ACNGT 3 cut(s) 296, 704, 1259
TaiI ACGT 2 cut(s) 1041, 1381
TaqI TCGA 5 cut(s) 21, 46, 60, 180, 1352
TaqII GACCGA 1 cut(s) 201
TasI AATT 9 cut(s) 280, 340, 347, 416, 683, 869, 998, 1054, 1077
TatI WGTACW 1 cut(s) 380
TauI GCSGC 2 cut(s) 173, 997
TfiI GAWTC 1 cut(s) 149
Tru1I TTAA 2 cut(s) 252, 780
Tru9I TTAA 2 cut(s) 252, 780
TscAI CASTG 1 cut(s) 945
TseFI GTSAC 1 cut(s) 274
TseI GCWGC 2 cut(s) 431, 1024
Tsp45I GTSAC 1 cut(s) 274
TspDTI ATGAA 2 cut(s) 569, 1080
TspRI CASTG 1 cut(s) 945
Van91I CCANNNNNTGG 1 cut(s) 588
XapI RAATTY 2 cut(s) 340, 683
XceI RCATGY 1 cut(s) 381
XcmI CCANNNNNNNNNTGG 1 cut(s) 340
XhoI CTCGAG 1 cut(s) 20
XspI CTAG 4 cut(s) 206, 285, 357, 842
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.