AT1G30280

Heat shock protein binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006648
Physical Location & Seq
Reverse (-)
10662608 .. 10664668
2061 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G30280.2

Sequence Viewer

Length: 1368 bp
ATGGACGAATCTTGGCGTATGAAGATGGGTTTAAGCGTTGATCCATTCTTCTCCATTGCTCGTAAATCAATGGATGCTCGTATCGACGCGGAAGACTTCGCTGATGTTTTCGGCGGTCCGCCGCGAAGCGTTCTCACCCGAAAATTTTCCGGCGACTTCTCTCGTTCCGACTGTTTCTACGACGAGATTTTTCAGCCTCCGGGAACTTTCTCCTCCGGCGGCTCTCTTGCGTCTTCTAAATCTCACGGAAGAAACTTACCGGCATTCAGAATTCCGTCCGGCGGGGAAGGATTTTACGACGGCGTGTTTGGTGGTCGCGGCGGTTCTGCGAAGGAAGGATCGAAAAAACAGAGCTCGATGACGAAACCAAGATCGAATTCATCGTCGATGCTCTCTTCCGAGGAGGCCAGTCCTCACTATCCTCCACCAGCGGCAGCTTCCGGCGACGACTCCGGATTCTCTTCTTTCACTTCTAGACTTAGACCGTTAAACGTCCCGTCGAGAAGTCATAAACGAGAGTCAAAGAAACAGAGTTTCTCGGCGGTTCCAAAAGCAAAAGATTCATTTTCCGGCCAAAATAACACACCGGAAAAAGCTGATTTCTACTACAAAAAAACACATTTCGGCGGATCAAGAAGAGCCTCGCCAGAAACCATAAGCTTGGATCCGAATTCCTTCAGAAGAATGGATGACTACGGACCAAGCTCCCCTGCATCTTCCCCTGTTTCTTCCTTCATCTGCGAAGAAGAAGACGACAACACTCATGCTAAACAGAGAACAAACAGAGACTGTAAAGTCGAAGAGGTAGTAGTAGAAGATGAAGAAGACGAAGAAGAAGAAGAGATGAGCTCTTACGTCATCGAGATAAACTCAGATCGATTCGACCGATACAGAGAAGAAGGAGGAGGAGGAGGAGGAAATTCAGATTCAAACGACATGGATGAAGCAATAGCTTGGGCTAAAGAAAGATCTCAAAGACCAGAAGCAAAGCAAACAGAAGAAGATGTAATCGATTCGAGAAGAAGTGAAGAAGAACCCAAATCAGAAGAAGAGATGGAGATGGAGATGAAAGATGAAGAGATAAGAATCTGGTTAACCGGAAAAGAGACAAACATTAGACTTCTCCTCTCAACTTTACATCATGTTCTTTGGTCAAATAGCAATTGGGATTCGATTCCTTTAGCAAATCTTCGAGATGGATCACAAGTGAAGAAAGCTTATCAAAGAGCTCGGCTTTGTTTACATCCAGATAAGCTACAACAAAGAGGAGGAACTTCACCGATTCAAAAATCTGTCGCGAGTAGAGTTTTCGCCATTCTTCAGGAAGCATGGGCTGTGTACGTAACAAATGAAGGACTCTCGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

455

Amino Acids

50.71

Weight (kDa)

5.18

Isoelectric Point (pI)

75.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 794
AccII CGCG 4 cut(s) 89, 124, 318, 1298
AccIII TCCGGA 1 cut(s) 452
AclWI GGATC 6 cut(s) 35, 346, 637, 659, 672, 1207
AcoI YGGCCR 1 cut(s) 571
AcsI RAATTY 5 cut(s) 143, 270, 376, 670, 919
AcuI CTGAAG 2 cut(s) 661, 1304
AfaI GTAC 1 cut(s) 1340
AfiI CCNNNNNNNGG 1 cut(s) 281
AgsI TTSAA 2 cut(s) 930, 1286
AjuI GAANNNNNNNTTGG 2 cut(s) 361, 393
Alw21I GWGCWC 3 cut(s) 356, 851, 1231
Alw26I GTCTC 2 cut(s) 780, 1100
AlwI GGATC 6 cut(s) 35, 346, 637, 659, 672, 1207
AlwNI CAGNNNCTG 1 cut(s) 789
Ama87I CYCGRG 1 cut(s) 1360
Aor13HI TCCGGA 1 cut(s) 452
AoxI GGCC 2 cut(s) 405, 571
ApeKI GCWGC 1 cut(s) 434
ApoI RAATTY 5 cut(s) 143, 270, 376, 670, 919
ArsI GACNNNNNNTTYG 4 cut(s) 290, 322, 368, 400
Asp700I GAANNNNTTC 2 cut(s) 145, 674
AspS9I GGNCC 2 cut(s) 116, 698
AsuC2I CCSGG 1 cut(s) 201
AsuHPI GGTGA 2 cut(s) 127, 1269
AvaI CYCGRG 1 cut(s) 1360
AvaII GGWCC 2 cut(s) 116, 698
BamHI GGATCC 1 cut(s) 664
BanII GRGCYC 3 cut(s) 356, 851, 1231
BbsI GAAGAC 4 cut(s) 99, 225, 756, 831
Bbv12I GWGCWC 3 cut(s) 356, 851, 1231
BbvI GCAGC 1 cut(s) 446
BccI CCATC 4 cut(s) 19, 1048, 1054, 1190
BceAI ACGGC 1 cut(s) 316
BcnI CCSGG 1 cut(s) 201
BcoDI GTCTC 2 cut(s) 780, 1100
BfaI CTAG 1 cut(s) 474
BglII AGATCT 1 cut(s) 968
BisI GCNGC 5 cut(s) 122, 220, 319, 432, 435
BlsI GCNGC 5 cut(s) 123, 221, 320, 433, 436
Bme1390I CCNGG 1 cut(s) 201
Bme18I GGWCC 2 cut(s) 116, 698
BmeT110I CYCGRG 1 cut(s) 1360
BmgT120I GGNCC 2 cut(s) 116, 698
BmiI GGNNCC 2 cut(s) 546, 666
BmrFI CCNGG 1 cut(s) 201
BmsI GCATC 3 cut(s) 64, 378, 722
BpiI GAAGAC 4 cut(s) 99, 225, 756, 831
BplI GAGNNNNNCTC 4 cut(s) 833, 865, 854, 886
BpuMI CCSGG 1 cut(s) 201
Bsa29I ATCGAT 2 cut(s) 877, 1011
BsaAI YACGTR 1 cut(s) 1342
BsaBI GATNNNNATC 1 cut(s) 1085
BsaJI CCNNGG 1 cut(s) 399
BsaWI WCCGGW 3 cut(s) 452, 586, 1097
Bsc4I CCNNNNNNNGG 1 cut(s) 281
Bse118I RCCGGY 1 cut(s) 259
Bse1I ACTGG 1 cut(s) 408
Bse3DI GCAATG 1 cut(s) 54
Bse8I GATNNNNATC 1 cut(s) 1085
BseAI TCCGGA 1 cut(s) 452
BseCI ATCGAT 2 cut(s) 877, 1011
BseDI CCNNGG 1 cut(s) 399
BseGI GGATG 4 cut(s) 79, 694, 946, 1243
BseJI GATNNNNATC 1 cut(s) 1085
BseLI CCNNNNNNNGG 1 cut(s) 281
BseMI GCAATG 1 cut(s) 54
BseMII CTCAG 1 cut(s) 885
BseNI ACTGG 1 cut(s) 408
BseRI GAGGAG 8 cut(s) 202, 416, 918, 921, 924, 927, 1115, 1281
BseXI GCAGC 1 cut(s) 446
Bsh1236I CGCG 4 cut(s) 89, 124, 318, 1298
Bsh1285I CGRYCG 1 cut(s) 886
BshFI GGCC 2 cut(s) 407, 573
BshVI ATCGAT 2 cut(s) 877, 1011
BsiEI CGRYCG 1 cut(s) 886
BsiHKAI GWGCWC 3 cut(s) 356, 851, 1231
BsiHKCI CYCGRG 1 cut(s) 1360
BslFI GGGAC 1 cut(s) 479
BslI CCNNNNNNNGG 1 cut(s) 281
BsmAI GTCTC 2 cut(s) 780, 1100
BsmFI GGGAC 1 cut(s) 479
BsmI GAATGC 1 cut(s) 263
BsnI GGCC 2 cut(s) 407, 573
BsoBI CYCGRG 1 cut(s) 1360
Bsp1286I GDGCHC 3 cut(s) 356, 851, 1231
Bsp13I TCCGGA 1 cut(s) 452
Bsp143I GATC 8 cut(s) 40, 338, 371, 629, 664, 874, 968, 1199
Bsp68I TCGCGA 1 cut(s) 1298
BspANI GGCC 2 cut(s) 407, 573
BspCNI CTCAG 1 cut(s) 884
BspDI ATCGAT 2 cut(s) 877, 1011
BspEI TCCGGA 1 cut(s) 452
BspFNI CGCG 4 cut(s) 89, 124, 318, 1298
BspLI GGNNCC 2 cut(s) 546, 666
BspPI GGATC 6 cut(s) 35, 346, 637, 659, 672, 1207
BspQI GCTCTTC 1 cut(s) 631
BsrDI GCAATG 1 cut(s) 54
BsrFI RCCGGY 1 cut(s) 259
BsrI ACTGG 1 cut(s) 408
BssAI RCCGGY 1 cut(s) 259
BssECI CCNNGG 1 cut(s) 399
BssMI GATC 8 cut(s) 40, 338, 371, 629, 664, 874, 968, 1199
Bst4CI ACNGT 3 cut(s) 173, 486, 791
Bst6I CTCTTC 7 cut(s) 400, 466, 631, 795, 834, 1044, 1071
BstBAI YACGTR 1 cut(s) 1342
BstDEI CTNAG 2 cut(s) 479, 871
BstF5I GGATG 4 cut(s) 79, 694, 946, 1243
BstFNI CGCG 4 cut(s) 89, 124, 318, 1298
BstKTI GATC 8 cut(s) 43, 341, 374, 632, 667, 877, 971, 1202
BstMAI GTCTC 2 cut(s) 780, 1100
BstMBI GATC 8 cut(s) 40, 338, 371, 629, 664, 874, 968, 1199
BstMCI CGRYCG 1 cut(s) 886
BstSCI CCNGG 1 cut(s) 199
BstSNI TACGTA 1 cut(s) 1342
BstUI CGCG 4 cut(s) 89, 124, 318, 1298
BstV1I GCAGC 1 cut(s) 446
BstV2I GAAGAC 4 cut(s) 99, 225, 756, 831
BstX2I RGATCY 2 cut(s) 664, 968
BstXI CCANNNNNNTGG 1 cut(s) 661
BstYI RGATCY 2 cut(s) 664, 968
Bsu15I ATCGAT 2 cut(s) 877, 1011
BsuRI GGCC 2 cut(s) 407, 573
BsuTUI ATCGAT 2 cut(s) 877, 1011
BtsCI GGATG 4 cut(s) 79, 694, 946, 1243
BtuMI TCGCGA 1 cut(s) 1298
CaiI CAGNNNCTG 1 cut(s) 789
Cfr10I RCCGGY 1 cut(s) 259
Cfr13I GGNCC 2 cut(s) 116, 698
ClaI ATCGAT 2 cut(s) 877, 1011
CpoI CGGWCCG 1 cut(s) 116
CseI GACGC 2 cut(s) 95, 219
Csp6I GTAC 1 cut(s) 1339
CspI CGGWCCG 1 cut(s) 116
CviAII CATG 4 cut(s) 764, 937, 1142, 1329
CviQI GTAC 1 cut(s) 1339
DdeI CTNAG 2 cut(s) 479, 871
DpnI GATC 8 cut(s) 42, 340, 373, 631, 666, 876, 970, 1201
DpnII GATC 8 cut(s) 40, 338, 371, 629, 664, 874, 968, 1199
DrdI GACNNNNNNGTC 1 cut(s) 794
DseDI GACNNNNNNGTC 1 cut(s) 794
EaeI YGGCCR 1 cut(s) 571
Eam1104I CTCTTC 7 cut(s) 400, 466, 631, 795, 834, 1044, 1071
EarI CTCTTC 7 cut(s) 400, 466, 631, 795, 834, 1044, 1071
EciI GGCGGA 2 cut(s) 108, 642
Ecl136II GAGCTC 3 cut(s) 354, 849, 1229
Eco105I TACGTA 1 cut(s) 1342
Eco24I GRGCYC 3 cut(s) 356, 851, 1231
Eco47I GGWCC 2 cut(s) 116, 698
Eco53kI GAGCTC 3 cut(s) 354, 849, 1229
Eco57I CTGAAG 2 cut(s) 661, 1304
Eco88I CYCGRG 1 cut(s) 1360
EcoICRI GAGCTC 3 cut(s) 354, 849, 1229
EcoRI GAATTC 3 cut(s) 270, 376, 670
EcoT38I GRGCYC 3 cut(s) 356, 851, 1231
FaeI CATG 4 cut(s) 767, 940, 1145, 1332
FaiI YATR 7 cut(s) 20, 510, 656, 765, 938, 1143, 1330
FaqI GGGAC 1 cut(s) 479
FatI CATG 4 cut(s) 763, 936, 1141, 1328
FauI CCCGC 1 cut(s) 275
Fnu4HI GCNGC 5 cut(s) 122, 220, 319, 432, 435
FokI GGATG 4 cut(s) 86, 701, 953, 1230
FriOI GRGCYC 3 cut(s) 356, 851, 1231
Fsp4HI GCNGC 5 cut(s) 122, 220, 319, 432, 435
FspBI CTAG 1 cut(s) 474
GluI GCNGC 5 cut(s) 122, 220, 319, 432, 435
HaeIII GGCC 2 cut(s) 407, 573
HgaI GACGC 2 cut(s) 95, 219
Hin1II CATG 4 cut(s) 767, 940, 1145, 1332
HincII GTYRAC 1 cut(s) 1095
HindII GTYRAC 1 cut(s) 1095
HindIII AAGCTT 2 cut(s) 658, 1215
HpaI GTTAAC 1 cut(s) 1095
HphI GGTGA 2 cut(s) 127, 1269
Hpy166II GTNNAC 3 cut(s) 1095, 1241, 1339
Hpy188I TCNGA 8 cut(s) 169, 269, 400, 669, 680, 874, 925, 1045
Hpy8I GTNNAC 3 cut(s) 1095, 1241, 1339
Hpy99I CGWCG 6 cut(s) 89, 185, 302, 388, 449, 502
HpyAV CCTTC 7 cut(s) 281, 325, 329, 685, 742, 893, 1346
HpyCH4III ACNGT 3 cut(s) 173, 486, 791
HpyCH4IV ACGT 3 cut(s) 492, 855, 1341
HpyCH4V TGCA 1 cut(s) 713
HpyF3I CTNAG 2 cut(s) 479, 871
HpySE526I ACGT 3 cut(s) 492, 855, 1341
Hsp92II CATG 4 cut(s) 767, 940, 1145, 1332
Kpn2I TCCGGA 1 cut(s) 452
KspAI GTTAAC 1 cut(s) 1095
Kzo9I GATC 8 cut(s) 40, 338, 371, 629, 664, 874, 968, 1199
LguI GCTCTTC 1 cut(s) 631
LmnI GCTCC 1 cut(s) 710
Lsp1109I GCAGC 1 cut(s) 446
LweI GCATC 3 cut(s) 64, 378, 722
MaeI CTAG 1 cut(s) 474
MaeII ACGT 3 cut(s) 492, 855, 1341
MaeIII GTNAC 1 cut(s) 1342
MalI GATC 8 cut(s) 42, 340, 373, 631, 666, 876, 970, 1201
MboI GATC 8 cut(s) 40, 338, 371, 629, 664, 874, 968, 1199
MfeI CAATTG 1 cut(s) 1162
MflI RGATCY 2 cut(s) 664, 968
MhlI GDGCHC 3 cut(s) 356, 851, 1231
MluCI AATT 6 cut(s) 143, 270, 376, 670, 919, 1162
MlyI GAGTC 3 cut(s) 443, 527, 1350
MmeI TCCRAC 1 cut(s) 192
MroI TCCGGA 1 cut(s) 452
MroXI GAANNNNTTC 2 cut(s) 145, 674
MseI TTAA 3 cut(s) 32, 488, 1094
MspA1I CMGCKG 1 cut(s) 431
MspR9I CCNGG 1 cut(s) 201
MunI CAATTG 1 cut(s) 1162
Mva1269I GAATGC 1 cut(s) 263
MvnI CGCG 4 cut(s) 89, 124, 318, 1298
NciI CCSGG 1 cut(s) 201
NdeII GATC 8 cut(s) 40, 338, 371, 629, 664, 874, 968, 1199
NlaIII CATG 4 cut(s) 767, 940, 1145, 1332
NlaIV GGNNCC 2 cut(s) 546, 666
NmeAIII GCCGAG 2 cut(s) 518, 1210
NruI TCGCGA 1 cut(s) 1298
PaeR7I CTCGAG 1 cut(s) 1360
PciSI GCTCTTC 1 cut(s) 631
PcsI WCGNNNNNNNCGW 2 cut(s) 380, 883
PctI GAATGC 1 cut(s) 263
PdmI GAANNNNTTC 2 cut(s) 145, 674
PfoI TCCNGGA 1 cut(s) 199
PkrI GCNGC 5 cut(s) 123, 221, 320, 433, 436
PleI GAGTC 3 cut(s) 443, 526, 1350
PpsI GAGTC 3 cut(s) 443, 526, 1350
Ppu21I YACGTR 1 cut(s) 1342
Psp124BI GAGCTC 3 cut(s) 356, 851, 1231
PspN4I GGNNCC 2 cut(s) 546, 666
PspPI GGNCC 2 cut(s) 116, 698
PstNI CAGNNNCTG 1 cut(s) 789
PsuI RGATCY 2 cut(s) 664, 968
RruI TCGCGA 1 cut(s) 1298
RsaI GTAC 1 cut(s) 1340
RsaNI GTAC 1 cut(s) 1339
Rsr2I CGGWCCG 1 cut(s) 116
RsrII CGGWCCG 1 cut(s) 116
SacI GAGCTC 3 cut(s) 356, 851, 1231
SapI GCTCTTC 1 cut(s) 631
SaqAI TTAA 3 cut(s) 32, 488, 1094
SatI GCNGC 5 cut(s) 122, 220, 319, 432, 435
Sau3AI GATC 8 cut(s) 40, 338, 371, 629, 664, 874, 968, 1199
Sau96I GGNCC 2 cut(s) 116, 698
SchI GAGTC 3 cut(s) 443, 527, 1350
ScrFI CCNGG 1 cut(s) 201
SduI GDGCHC 3 cut(s) 356, 851, 1231
SfaNI GCATC 3 cut(s) 64, 378, 722
Sfr274I CTCGAG 1 cut(s) 1360
SinI GGWCC 2 cut(s) 116, 698
SlaI CTCGAG 1 cut(s) 1360
SmlI CTYRAG 1 cut(s) 1360
SmoI CTYRAG 1 cut(s) 1360
SnaBI TACGTA 1 cut(s) 1342
Sse9I AATT 6 cut(s) 143, 270, 376, 670, 919, 1162
SspMI CTAG 1 cut(s) 474
SstI GAGCTC 3 cut(s) 356, 851, 1231
StyD4I CCNGG 1 cut(s) 199
TaaI ACNGT 3 cut(s) 173, 486, 791
TaiI ACGT 3 cut(s) 495, 858, 1344
TaqII GACCGA 1 cut(s) 900
TasI AATT 6 cut(s) 143, 270, 376, 670, 919, 1162
TauI GCSGC 4 cut(s) 124, 222, 321, 434
Tru1I TTAA 3 cut(s) 32, 488, 1094
Tru9I TTAA 3 cut(s) 32, 488, 1094
TseI GCWGC 1 cut(s) 434
TspDTI ATGAA 9 cut(s) 35, 369, 552, 724, 834, 957, 1082, 1089, 1365
TspGWI ACGGA 3 cut(s) 261, 264, 711
VpaK11BI GGWCC 2 cut(s) 116, 698
XapI RAATTY 5 cut(s) 143, 270, 376, 670, 919
XbaI TCTAGA 1 cut(s) 473
XhoI CTCGAG 1 cut(s) 1360
XmnI GAANNNNTTC 2 cut(s) 145, 674
XspI CTAG 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.