Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0012999

Physical Location & Seq

Genomic Coordinates Forward (+)

11607854 .. 11609968

Gene Structure 2115 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G32220.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 891 bp

ATGACTTCGTTTCTTAGTTTCTCCGCCATTTCAGCTCATCCACCTACTTTTTCCGGCGCTTCATTTCGTCCTCGCTCTTTCTCTCCCCGATTGTTCAAGTCTTGTGTTAAGTGCACTTATGCTGAAGCTGGATTAAGCAGCGCTTCATGGTCAGCCCCTATTGACATTGTGGCAGATGTCAAATCCGAAAGGGTTGTAGTTCTTGGTGGGAATGGTTTCGTTGGCTCAGCTATCTGCAAAGCAGCAATCTCCAATGGTATTGAGGTTGTGAGTGTTAGCAGGTCAGGTCGTCCTAACTTCGAAGATTCATGGTTGGATCAGGTTACATGGGTTACTGGTGATGTTTTCTATTTGAATTGGGATGAAGTACTTCTTGGTGCTACTGCTGTAGTTTCAACCATTGGTGGTTTTGGAAATGAAGAACAGATGAAAAGAATCAATGGTGAAGCTAACGTTACCGCTGTGAATGCTGCTAAGGATTTTGGGGTCCCTAAGTTCGTCTTGATCACGGTTCACGATTACAATCTTCCGCCATTTATTCTCTCCAACGGATATTTCACTGGAAAACGTAACGCGGAGGCAGAACTTCTTTCCAAGTATCCCACCTCAGGAGTTGTGCTAAGACCCGGTTTCATATACGGGAAACGAAAAGTGAACGGAATCGAGGTCCCGCTTGATCTAGTCGGGGAGCCACTAGACAAGATCTATGATTCAGCAGAGAGGTTCATTAGACCATTGAGGTCTCTCCCTGCATCTGATCTCATCTTGGCTCCACCGGTTAACGTCGATGATTTAGCACTTGCTGTGATCAACGCTGTTAAAGATGACGACTTCTTTGGCATTTTCACTATTGAGCAGATCAAAGAAGCAGCTGCAAAAATGAGAGCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003954 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006732 GO:0006733 GO:0006743 GO:0006744 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009987 GO:0010287 GO:0016491 GO:0016651 GO:0042180 GO:0042181 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051186 GO:0051188 GO:0055114 GO:0071704 GO:1901004 GO:1901006 GO:1901576 GO:1901661 GO:1901663

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NAD_binding_10

Protein Analysis

296

Amino Acids

31.98

Weight (kDa)

5.56

Isoelectric Point (pI)

36.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Epimerase	PF01370	65 - 169	1.1e-07	NAD dependent epimerase/dehydratase family
NAD_binding_10	PF13460	69 - 213	4.1e-10	NAD(P)H-binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012999)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G32220
fragaria_vesca	FvH4_5g23050 FvH4_5g23050
malus_domestica	MD04G1103200.v1.1
pyrus_communis	pycom04g09830
rosa_chinensis	RchiOBHm_Chr7g0211771
rosa_laevigata	RLG00000002970
rosa_multiflora	Rmu_sc0014977.1_g000002 Rmu_sc0015845.1_g000001 Rmu_sc0016160.1_g000001
rosa_roxburghii	Rroxscaffold_3G00247460
rosa_rugosa	Rorug07G0128500 Rorug07G0128500
rosa_samantha	Rh7AG261200 Rh7BG254700 Rh7CG277800 Rh7DG267800
rosa_wichuraiana	Rw7G022260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	270
AccII	CGCG	1 cut(s)	575
AciI	CCGC	5 cut(s)	24, 459, 530, 575, 671
AclI	AACGTT	1 cut(s)	453
AclWI	GGATC	1 cut(s)	324
AcuI	CTGAAG	1 cut(s)	144
AfaI	GTAC	1 cut(s)	369
AfeI	AGCGCT	1 cut(s)	142
AfiI	CCNNNNNNNGG	1 cut(s)	608
AgeI	ACCGGT	1 cut(s)	775
AgsI	TTSAA	3 cut(s)	97, 355, 396
AjuI	GAANNNNNNNTTGG	4 cut(s)	357, 389, 539, 571
AluBI	AGCT	5 cut(s)	35, 128, 230, 449, 872
AluI	AGCT	5 cut(s)	35, 128, 230, 449, 872
Alw21I	GWGCWC	1 cut(s)	116
Alw26I	GTCTC	1 cut(s)	747
Alw44I	GTGCAC	1 cut(s)	112
AlwI	GGATC	1 cut(s)	324
Aor51HI	AGCGCT	1 cut(s)	142
ApaLI	GTGCAC	1 cut(s)	112
ApeKI	GCWGC	5 cut(s)	138, 242, 470, 869, 872
ArsI	GACNNNNNNTTYG	2 cut(s)	818, 850
AsiGI	ACCGGT	1 cut(s)	775
Asp700I	GAANNNNTTC	2 cut(s)	215, 369
AspLEI	GCGC	2 cut(s)	59, 143
AspS9I	GGNCC	2 cut(s)	487, 667
AsuC2I	CCSGG	1 cut(s)	627
AsuHPI	GGTGA	2 cut(s)	350, 455
AsuII	TTCGAA	1 cut(s)	300
AvaII	GGWCC	2 cut(s)	487, 667
AxyI	CCTNAGG	1 cut(s)	607
BaeGI	GKGCMC	1 cut(s)	116
Bbv12I	GWGCWC	1 cut(s)	116
BbvI	GCAGC	5 cut(s)	150, 254, 457, 859, 881
BciVI	GTATCC	1 cut(s)	609
BclI	TGATCA	2 cut(s)	504, 807
BcnI	CCSGG	1 cut(s)	627
BcoDI	GTCTC	1 cut(s)	747
BfaI	CTAG	2 cut(s)	680, 695
BfmI	CTRYAG	1 cut(s)	387
BfoI	RGCGCY	2 cut(s)	60, 144
BfuAI	ACCTGC	1 cut(s)	270
BfuI	GTATCC	1 cut(s)	609
BglII	AGATCT	1 cut(s)	702
BisI	GCNGC	5 cut(s)	139, 243, 471, 870, 873
BlpI	GCTNAGC	1 cut(s)	226
BlsI	GCNGC	5 cut(s)	140, 244, 472, 871, 874
BmcAI	AGTACT	1 cut(s)	369
Bme1390I	CCNGG	1 cut(s)	627
Bme18I	GGWCC	2 cut(s)	487, 667
BmgT120I	GGNCC	2 cut(s)	487, 667
BmiI	GGNNCC	5 cut(s)	488, 489, 669, 690, 771
BmrFI	CCNGG	1 cut(s)	627
BmsI	GCATC	1 cut(s)	761
Bpu10I	CCTNAGC	1 cut(s)	474
Bpu1102I	GCTNAGC	1 cut(s)	226
Bpu14I	TTCGAA	1 cut(s)	300
BpuMI	CCSGG	1 cut(s)	627
BsaBI	GATNNNNATC	1 cut(s)	522
BsaI	GGTCTC	1 cut(s)	747
BsaWI	WCCGGW	1 cut(s)	775
Bsc4I	CCNNNNNNNGG	1 cut(s)	608
Bse118I	RCCGGY	1 cut(s)	775
Bse1I	ACTGG	2 cut(s)	340, 565
Bse21I	CCTNAGG	1 cut(s)	607
Bse8I	GATNNNNATC	1 cut(s)	522
BseGI	GGATG	2 cut(s)	37, 367
BseJI	GATNNNNATC	1 cut(s)	522
BseLI	CCNNNNNNNGG	1 cut(s)	608
BseMII	CTCAG	2 cut(s)	240, 621
BseNI	ACTGG	2 cut(s)	340, 565
BseSI	GKGCMC	1 cut(s)	116
BseXI	GCAGC	5 cut(s)	150, 254, 457, 859, 881
Bsh1236I	CGCG	1 cut(s)	575
BshTI	ACCGGT	1 cut(s)	775
BsiHKAI	GWGCWC	1 cut(s)	116
BsiSI	CCGG	3 cut(s)	54, 627, 776
BslFI	GGGAC	2 cut(s)	473, 653
BslI	CCNNNNNNNGG	1 cut(s)	608
BsmAI	GTCTC	1 cut(s)	747
BsmFI	GGGAC	2 cut(s)	473, 653
BsmI	GAATGC	1 cut(s)	472
Bso31I	GGTCTC	1 cut(s)	747
Bsp119I	TTCGAA	1 cut(s)	300
Bsp1286I	GDGCHC	1 cut(s)	116
Bsp143I	GATC	7 cut(s)	316, 504, 676, 702, 757, 807, 858
Bsp1720I	GCTNAGC	1 cut(s)	226
BspACI	CCGC	5 cut(s)	24, 459, 530, 575, 671
BspCNI	CTCAG	2 cut(s)	239, 620
BspFNI	CGCG	1 cut(s)	575
BspLI	GGNNCC	5 cut(s)	488, 489, 669, 690, 771
BspMI	ACCTGC	1 cut(s)	270
BspPI	GGATC	1 cut(s)	324
BspT104I	TTCGAA	1 cut(s)	300
BspTNI	GGTCTC	1 cut(s)	747
BsrFI	RCCGGY	1 cut(s)	775
BsrI	ACTGG	2 cut(s)	340, 565
BssAI	RCCGGY	1 cut(s)	775
BssMI	GATC	7 cut(s)	316, 504, 676, 702, 757, 807, 858
Bst4CI	ACNGT	1 cut(s)	511
BstBI	TTCGAA	1 cut(s)	300
BstDEI	CTNAG	6 cut(s)	14, 226, 474, 492, 607, 620
BstF5I	GGATG	2 cut(s)	37, 367
BstFNI	CGCG	1 cut(s)	575
BstH2I	RGCGCY	2 cut(s)	60, 144
BstHHI	GCGC	2 cut(s)	59, 143
BstKTI	GATC	7 cut(s)	319, 507, 679, 705, 760, 810, 861
BstMAI	GTCTC	1 cut(s)	747
BstMBI	GATC	7 cut(s)	316, 504, 676, 702, 757, 807, 858
BstMWI	GCNNNNNNNGC	2 cut(s)	32, 467
BstSCI	CCNGG	1 cut(s)	625
BstSFI	CTRYAG	1 cut(s)	387
BstSLI	GKGCMC	1 cut(s)	116
BstUI	CGCG	1 cut(s)	575
BstV1I	GCAGC	5 cut(s)	150, 254, 457, 859, 881
BstX2I	RGATCY	1 cut(s)	702
BstYI	RGATCY	1 cut(s)	702
Bsu36I	CCTNAGG	1 cut(s)	607
BsuI	GTATCC	1 cut(s)	609
BtsCI	GGATG	2 cut(s)	37, 367
BtsIMutI	CAGTG	1 cut(s)	558
BveI	ACCTGC	1 cut(s)	270
CfoI	GCGC	2 cut(s)	59, 143
Cfr10I	RCCGGY	1 cut(s)	775
Cfr13I	GGNCC	2 cut(s)	487, 667
Csp6I	GTAC	1 cut(s)	368
CspAI	ACCGGT	1 cut(s)	775
CviAII	CATG	3 cut(s)	147, 309, 327
CviJI	RGCY	9 cut(s)	35, 128, 155, 225, 230, 449, 691, 770, 872
CviKI_1	RGCY	9 cut(s)	35, 128, 155, 225, 230, 449, 691, 770, 872
CviQI	GTAC	1 cut(s)	368
DdeI	CTNAG	6 cut(s)	14, 226, 474, 492, 607, 620
DpnI	GATC	7 cut(s)	318, 506, 678, 704, 759, 809, 860
DpnII	GATC	7 cut(s)	316, 504, 676, 702, 757, 807, 858
EciI	GGCGGA	2 cut(s)	13, 519
Eco31I	GGTCTC	1 cut(s)	747
Eco47I	GGWCC	2 cut(s)	487, 667
Eco47III	AGCGCT	1 cut(s)	142
Eco57I	CTGAAG	1 cut(s)	144
Eco81I	CCTNAGG	1 cut(s)	607
EcoO109I	RGGNCCY	2 cut(s)	487, 667
FaeI	CATG	3 cut(s)	150, 312, 330
FaiI	YATR	7 cut(s)	120, 148, 310, 328, 635, 637, 708
FalI	AAGNNNNNCTT	6 cut(s)	127, 159, 357, 389, 485, 517
FaqI	GGGAC	2 cut(s)	473, 653
FatI	CATG	3 cut(s)	146, 308, 326
FauI	CCCGC	1 cut(s)	678
FbaI	TGATCA	2 cut(s)	504, 807
Fnu4HI	GCNGC	5 cut(s)	139, 243, 471, 870, 873
FokI	GGATG	2 cut(s)	24, 374
Fsp4HI	GCNGC	5 cut(s)	139, 243, 471, 870, 873
FspBI	CTAG	2 cut(s)	680, 695
GlaI	GCGC	2 cut(s)	58, 142
GluI	GCNGC	5 cut(s)	139, 243, 471, 870, 873
HaeII	RGCGCY	2 cut(s)	60, 144
HapII	CCGG	3 cut(s)	54, 627, 776
HhaI	GCGC	2 cut(s)	59, 143
Hin1II	CATG	3 cut(s)	150, 312, 330
Hin6I	GCGC	2 cut(s)	57, 141
HinP1I	GCGC	2 cut(s)	57, 141
HincII	GTYRAC	1 cut(s)	781
HindII	GTYRAC	1 cut(s)	781
HinfI	GANTC	4 cut(s)	305, 435, 660, 710
HpaI	GTTAAC	1 cut(s)	781
HpaII	CCGG	3 cut(s)	54, 627, 776
HphI	GGTGA	2 cut(s)	350, 455
Hpy166II	GTNNAC	4 cut(s)	114, 514, 655, 781
Hpy188I	TCNGA	2 cut(s)	187, 757
Hpy188III	TCNNGA	3 cut(s)	502, 515, 609
Hpy8I	GTNNAC	4 cut(s)	114, 514, 655, 781
Hpy99I	CGWCG	1 cut(s)	788
HpyCH4III	ACNGT	1 cut(s)	511
HpyCH4IV	ACGT	3 cut(s)	453, 568, 783
HpyCH4V	TGCA	4 cut(s)	114, 237, 752, 875
HpyF10VI	GCNNNNNNNGC	2 cut(s)	32, 467
HpyF3I	CTNAG	6 cut(s)	14, 226, 474, 492, 607, 620
HpySE526I	ACGT	3 cut(s)	453, 568, 783
Hsp92II	CATG	3 cut(s)	150, 312, 330
HspAI	GCGC	2 cut(s)	57, 141
KflI	GGGWCCC	1 cut(s)	487
Ksp22I	TGATCA	2 cut(s)	504, 807
KspAI	GTTAAC	1 cut(s)	781
Kzo9I	GATC	7 cut(s)	316, 504, 676, 702, 757, 807, 858
LmnI	GCTCC	2 cut(s)	688, 775
Lsp1109I	GCAGC	5 cut(s)	150, 254, 457, 859, 881
LweI	GCATC	1 cut(s)	761
MaeI	CTAG	2 cut(s)	680, 695
MaeII	ACGT	3 cut(s)	453, 568, 783
MaeIII	GTNAC	4 cut(s)	322, 331, 454, 569
MalI	GATC	7 cut(s)	318, 506, 678, 704, 759, 809, 860
MboI	GATC	7 cut(s)	316, 504, 676, 702, 757, 807, 858
MboII	GAAGA	3 cut(s)	314, 431, 518
MflI	RGATCY	1 cut(s)	702
MhlI	GDGCHC	1 cut(s)	116
MluCI	AATT	1 cut(s)	355
MmeI	TCCRAC	2 cut(s)	294, 570
MnlI	CCTC	7 cut(s)	81, 256, 571, 616, 658, 714, 732
MroXI	GAANNNNTTC	2 cut(s)	215, 369
MseI	TTAA	4 cut(s)	108, 134, 780, 819
MspA1I	CMGCKG	2 cut(s)	461, 872
MspI	CCGG	3 cut(s)	54, 627, 776
MspR9I	CCNGG	1 cut(s)	627
Mva1269I	GAATGC	1 cut(s)	472
MvnI	CGCG	1 cut(s)	575
MwoI	GCNNNNNNNGC	2 cut(s)	32, 467
NciI	CCSGG	1 cut(s)	627
NdeII	GATC	7 cut(s)	316, 504, 676, 702, 757, 807, 858
NlaIII	CATG	3 cut(s)	150, 312, 330
NlaIV	GGNNCC	5 cut(s)	488, 489, 669, 690, 771
NspV	TTCGAA	1 cut(s)	300
PctI	GAATGC	1 cut(s)	472
PdmI	GAANNNNTTC	2 cut(s)	215, 369
PfeI	GAWTC	4 cut(s)	305, 435, 660, 710
PinAI	ACCGGT	1 cut(s)	775
PkrI	GCNGC	5 cut(s)	140, 244, 472, 871, 874
PpuMI	RGGWCCY	2 cut(s)	487, 667
Psp1406I	AACGTT	1 cut(s)	453
Psp5II	RGGWCCY	2 cut(s)	487, 667
PspN4I	GGNNCC	5 cut(s)	488, 489, 669, 690, 771
PspPI	GGNCC	2 cut(s)	487, 667
PspPPI	RGGWCCY	2 cut(s)	487, 667
PsuI	RGATCY	1 cut(s)	702
PvuII	CAGCTG	1 cut(s)	872
RsaI	GTAC	1 cut(s)	369
RsaNI	GTAC	1 cut(s)	368
SaqAI	TTAA	4 cut(s)	108, 134, 780, 819
SatI	GCNGC	5 cut(s)	139, 243, 471, 870, 873
Sau3AI	GATC	7 cut(s)	316, 504, 676, 702, 757, 807, 858
Sau96I	GGNCC	2 cut(s)	487, 667
ScaI	AGTACT	1 cut(s)	369
ScrFI	CCNGG	1 cut(s)	627
SduI	GDGCHC	1 cut(s)	116
SfaNI	GCATC	1 cut(s)	761
SfcI	CTRYAG	1 cut(s)	387
SfuI	TTCGAA	1 cut(s)	300
SinI	GGWCC	2 cut(s)	487, 667
Sse9I	AATT	1 cut(s)	355
SsiI	CCGC	5 cut(s)	24, 459, 530, 575, 671
SspMI	CTAG	2 cut(s)	680, 695
StyD4I	CCNGG	1 cut(s)	625
TaaI	ACNGT	1 cut(s)	511
TaiI	ACGT	3 cut(s)	456, 571, 786
TaqI	TCGA	3 cut(s)	300, 663, 786
TasI	AATT	1 cut(s)	355
TatI	WGTACW	1 cut(s)	367
TfiI	GAWTC	4 cut(s)	305, 435, 660, 710
Tru1I	TTAA	4 cut(s)	108, 134, 780, 819
Tru9I	TTAA	4 cut(s)	108, 134, 780, 819
TscAI	CASTG	1 cut(s)	565
TseI	GCWGC	5 cut(s)	138, 242, 470, 869, 872
TspDTI	ATGAA	8 cut(s)	51, 135, 297, 378, 432, 443, 622, 715
TspGWI	ACGGA	2 cut(s)	564, 672
TspRI	CASTG	1 cut(s)	565
VneI	GTGCAC	1 cut(s)	112
VpaK11BI	GGWCC	2 cut(s)	487, 667
XmnI	GAANNNNTTC	2 cut(s)	215, 369
XspI	CTAG	2 cut(s)	680, 695
ZrmI	AGTACT	1 cut(s)	369

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.