Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0012999

Physical Location & Seq

Genomic Coordinates Reverse (-)

19065561 .. 19069501

Gene Structure 3941 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1103200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 891 bp

ATGTCGTCGTTTTTGCACTTCCCTGCCGTTTCTACGCTCCCCTCTTCTTTTCCCGGAGCTTTCTTCCCCGCCCGGCCCAAACCCACCTCGCTTTTCCACCACCGTGTTAAATGCAGATATGGAGAGGTGAATGTAAAGACGGATTTCAATTCCGCCACAATAGACGTTGTGGCCGATGTTAAACCTGAACGGGTTGTTGTGTTAGGAGGCACCGGTTTTGTTGGTTCTGCTATATGCAAGGCTGCGGTATCCAGAGGAATAGAGGTTGTTGGCGTGAGCAGGTCAGGGCGTCCTACTTACTCCGGTGCGTGGATAGATCAGGTTAACTGGGTGCCAGGAGATGTTTTCTATGTAAACTGGGATGAAGTACTTATCGGGGCCACCACTGTGGTTTCAACCATTGGTGGTTTTGGCAGCGAGGAACAGATGCAAAGGATTAATGGGGAGGCAAATGTTGTTGCTGTTAATGCTGCGAAGGATTACGGAGTTCCCAAGTTTATCTTGATCTCAGTGCATGATTACAATCTACCTCCATTTTTGCTTTCATCTGGGTACTTCACAGGAAAGAGAAAAGCTGAATCAGAGGTTCTCTCCAAATATCCCAATTCTGGTATCGTGCTTAGACCTGGTTTCATATACGGGAAACGGAGGGTGGATGGTTTTGAGATTCCTTTGGATTTGATAGGTGAACCATTGGAAAGATTTATTAATGCTACCGAAAACTTTACCAAACCGTTGAGTTCTCTTCCAGCATCTGATCTGCTCTTGGCTCCACCTGTTAGTGTCGATGATGTTGCACTTGCCGCAATCAATGGAATCAGAGATGATGATTTCTTCGGCATTTTTACCATTGCACAAATTAAGGAAGCTGCGGAGAAGGTGAGGGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003954 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006732 GO:0006733 GO:0006743 GO:0006744 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009987 GO:0010287 GO:0016491 GO:0016651 GO:0042180 GO:0042181 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051186 GO:0051188 GO:0055114 GO:0071704 GO:1901004 GO:1901006 GO:1901576 GO:1901661 GO:1901663

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NAD_binding_10

Protein Analysis

297

Amino Acids

32.06

Weight (kDa)

6.12

Isoelectric Point (pI)

31.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Epimerase	PF01370	65 - 216	3.5e-08	NAD dependent epimerase/dehydratase family
NAD_binding_10	PF13460	69 - 195	6.3e-09	NAD(P)H-binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012999)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G32220
fragaria_vesca	FvH4_5g23050 FvH4_5g23050
malus_domestica	MD04G1103200.v1.1
pyrus_communis	pycom04g09830
rosa_chinensis	RchiOBHm_Chr7g0211771
rosa_laevigata	RLG00000002970
rosa_multiflora	Rmu_sc0014977.1_g000002 Rmu_sc0015845.1_g000001 Rmu_sc0016160.1_g000001
rosa_roxburghii	Rroxscaffold_3G00247460
rosa_rugosa	Rorug07G0128500 Rorug07G0128500
rosa_samantha	Rh7AG261200 Rh7BG254700 Rh7CG277800 Rh7DG267800
rosa_wichuraiana	Rw7G022260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	270
AccB1I	GGYRCC	2 cut(s)	209, 331
AciI	CCGC	5 cut(s)	69, 153, 245, 804, 872
AcoI	YGGCCR	1 cut(s)	171
AcyI	GRCGYC	1 cut(s)	289
AfaI	GTAC	2 cut(s)	369, 554
AfiI	CCNNNNNNNGG	2 cut(s)	309, 608
AgeI	ACCGGT	1 cut(s)	212
AgsI	TTSAA	2 cut(s)	148, 396
AjnI	CCWGG	2 cut(s)	334, 625
AleI	CACNNNNGTG	2 cut(s)	102, 386
AluBI	AGCT	3 cut(s)	59, 575, 869
AluI	AGCT	3 cut(s)	59, 575, 869
AlwNI	CAGNNNCTG	1 cut(s)	755
AoxI	GGCC	3 cut(s)	74, 171, 378
ApeKI	GCWGC	4 cut(s)	242, 414, 470, 869
AseI	ATTAAT	2 cut(s)	438, 708
AsiGI	ACCGGT	1 cut(s)	212
AspS9I	GGNCC	2 cut(s)	75, 378
AsuC2I	CCSGG	2 cut(s)	54, 73
AsuHPI	GGTGA	2 cut(s)	139, 698
BanI	GGYRCC	2 cut(s)	209, 331
BbvI	GCAGC	4 cut(s)	229, 426, 457, 856
BccI	CCATC	1 cut(s)	650
BceAI	ACGGC	1 cut(s)	11
BcgI	CGANNNNNNTGC	2 cut(s)	776, 810
BciT130I	CCWGG	2 cut(s)	336, 627
BciVI	GTATCC	1 cut(s)	259
BcnI	CCSGG	2 cut(s)	54, 73
BfuAI	ACCTGC	1 cut(s)	270
BfuI	GTATCC	1 cut(s)	259
BisI	GCNGC	5 cut(s)	243, 415, 471, 804, 870
BlsI	GCNGC	5 cut(s)	244, 416, 472, 805, 871
BmcAI	AGTACT	1 cut(s)	369
Bme1390I	CCNGG	4 cut(s)	54, 73, 336, 627
BmgT120I	GGNCC	2 cut(s)	75, 378
BmiI	GGNNCC	4 cut(s)	211, 333, 379, 771
BmrFI	CCNGG	4 cut(s)	54, 73, 336, 627
BmrI	ACTGGG	2 cut(s)	337, 367
BmsI	GCATC	2 cut(s)	417, 761
BmuI	ACTGGG	2 cut(s)	337, 367
BplI	GAGNNNNNCTC	2 cut(s)	575, 607
BpuMI	CCSGG	2 cut(s)	54, 73
BsaBI	GATNNNNATC	1 cut(s)	522
BsaHI	GRCGYC	1 cut(s)	289
BsaWI	WCCGGW	2 cut(s)	212, 302
Bsc4I	CCNNNNNNNGG	2 cut(s)	309, 608
Bse118I	RCCGGY	1 cut(s)	212
Bse1I	ACTGG	2 cut(s)	332, 362
Bse3DI	GCAATG	1 cut(s)	849
Bse8I	GATNNNNATC	1 cut(s)	522
BseBI	CCWGG	2 cut(s)	336, 627
BseGI	GGATG	2 cut(s)	367, 661
BseJI	GATNNNNATC	1 cut(s)	522
BseLI	CCNNNNNNNGG	2 cut(s)	309, 608
BseMI	GCAATG	1 cut(s)	849
BseMII	CTCAG	1 cut(s)	522
BseNI	ACTGG	2 cut(s)	332, 362
BseXI	GCAGC	4 cut(s)	229, 426, 457, 856
BshFI	GGCC	3 cut(s)	76, 173, 380
BshNI	GGYRCC	2 cut(s)	209, 331
BshTI	ACCGGT	1 cut(s)	212
BsiSI	CCGG	4 cut(s)	54, 73, 213, 303
BslI	CCNNNNNNNGG	2 cut(s)	309, 608
BsnI	GGCC	3 cut(s)	76, 173, 380
Bsp143I	GATC	3 cut(s)	316, 504, 757
BspACI	CCGC	5 cut(s)	69, 153, 245, 804, 872
BspANI	GGCC	3 cut(s)	76, 173, 380
BspCNI	CTCAG	1 cut(s)	521
BspLI	GGNNCC	4 cut(s)	211, 333, 379, 771
BspMI	ACCTGC	1 cut(s)	270
BspT107I	GGYRCC	2 cut(s)	209, 331
BsrDI	GCAATG	1 cut(s)	849
BsrFI	RCCGGY	1 cut(s)	212
BsrI	ACTGG	2 cut(s)	332, 362
BssAI	RCCGGY	1 cut(s)	212
BssMI	GATC	3 cut(s)	316, 504, 757
BssNI	GRCGYC	1 cut(s)	289
Bst2UI	CCWGG	2 cut(s)	336, 627
Bst4CI	ACNGT	3 cut(s)	104, 388, 735
Bst6I	CTCTTC	2 cut(s)	49, 750
BstACI	GRCGYC	1 cut(s)	289
BstDEI	CTNAG	2 cut(s)	508, 620
BstF5I	GGATG	2 cut(s)	367, 661
BstKTI	GATC	3 cut(s)	319, 507, 760
BstMBI	GATC	3 cut(s)	316, 504, 757
BstMWI	GCNNNNNNNGC	2 cut(s)	467, 803
BstNI	CCWGG	2 cut(s)	336, 627
BstSCI	CCNGG	4 cut(s)	52, 71, 334, 625
BstV1I	GCAGC	4 cut(s)	229, 426, 457, 856
BstXI	CCANNNNNNTGG	1 cut(s)	388
BsuI	GTATCC	1 cut(s)	259
BsuRI	GGCC	3 cut(s)	76, 173, 380
BtsCI	GGATG	2 cut(s)	367, 661
BtsIMutI	CAGTG	2 cut(s)	384, 516
BveI	ACCTGC	1 cut(s)	270
CaiI	CAGNNNCTG	1 cut(s)	755
Cfr10I	RCCGGY	1 cut(s)	212
Cfr13I	GGNCC	2 cut(s)	75, 378
CseI	GACGC	1 cut(s)	278
CsiI	ACCWGGT	1 cut(s)	625
Csp6I	GTAC	2 cut(s)	368, 553
CspAI	ACCGGT	1 cut(s)	212
CviAII	CATG	1 cut(s)	515
CviJI	RGCY	8 cut(s)	59, 76, 173, 242, 380, 575, 770, 869
CviKI_1	RGCY	8 cut(s)	59, 76, 173, 242, 380, 575, 770, 869
CviQI	GTAC	2 cut(s)	368, 553
DdeI	CTNAG	2 cut(s)	508, 620
DpnI	GATC	3 cut(s)	318, 506, 759
DpnII	GATC	3 cut(s)	316, 504, 757
EaeI	YGGCCR	1 cut(s)	171
Eam1104I	CTCTTC	2 cut(s)	49, 750
EarI	CTCTTC	2 cut(s)	49, 750
EciI	GGCGGA	1 cut(s)	142
EcoRII	CCWGG	2 cut(s)	334, 625
FaeI	CATG	1 cut(s)	518
FaiI	YATR	7 cut(s)	120, 233, 235, 351, 516, 635, 637
FalI	AAGNNNNNCTT	2 cut(s)	485, 517
FatI	CATG	1 cut(s)	514
FauI	CCCGC	1 cut(s)	76
Fnu4HI	GCNGC	5 cut(s)	243, 415, 471, 804, 870
FokI	GGATG	2 cut(s)	374, 668
Fsp4HI	GCNGC	5 cut(s)	243, 415, 471, 804, 870
GluI	GCNGC	5 cut(s)	243, 415, 471, 804, 870
HaeIII	GGCC	3 cut(s)	76, 173, 380
HapII	CCGG	4 cut(s)	54, 73, 213, 303
HgaI	GACGC	1 cut(s)	278
Hin1I	GRCGYC	1 cut(s)	289
Hin1II	CATG	1 cut(s)	518
HincII	GTYRAC	1 cut(s)	325
HindII	GTYRAC	1 cut(s)	325
HinfI	GANTC	3 cut(s)	578, 667, 816
HpaI	GTTAAC	1 cut(s)	325
HpaII	CCGG	4 cut(s)	54, 73, 213, 303
HphI	GGTGA	2 cut(s)	139, 698
Hpy166II	GTNNAC	3 cut(s)	325, 355, 689
Hpy188I	TCNGA	3 cut(s)	583, 757, 821
Hpy188III	TCNNGA	2 cut(s)	252, 502
Hpy8I	GTNNAC	3 cut(s)	325, 355, 689
Hpy99I	CGWCG	1 cut(s)	10
HpyAV	CCTTC	2 cut(s)	469, 871
HpyCH4III	ACNGT	3 cut(s)	104, 388, 735
HpyCH4IV	ACGT	1 cut(s)	165
HpyCH4V	TGCA	7 cut(s)	16, 114, 237, 430, 514, 797, 854
HpyF10VI	GCNNNNNNNGC	2 cut(s)	467, 803
HpyF3I	CTNAG	2 cut(s)	508, 620
HpySE526I	ACGT	1 cut(s)	165
Hsp92I	GRCGYC	1 cut(s)	289
Hsp92II	CATG	1 cut(s)	518
KspAI	GTTAAC	1 cut(s)	325
Kzo9I	GATC	3 cut(s)	316, 504, 757
LmnI	GCTCC	3 cut(s)	42, 56, 775
Lsp1109I	GCAGC	4 cut(s)	229, 426, 457, 856
LweI	GCATC	2 cut(s)	417, 761
MabI	ACCWGGT	1 cut(s)	625
MaeII	ACGT	1 cut(s)	165
MalI	GATC	3 cut(s)	318, 506, 759
MboI	GATC	3 cut(s)	316, 504, 757
MboII	GAAGA	4 cut(s)	36, 55, 737, 826
MluCI	AATT	3 cut(s)	148, 604, 858
MseI	TTAA	7 cut(s)	108, 180, 324, 438, 465, 708, 861
MslI	CAYNNNNRTG	2 cut(s)	102, 386
MspI	CCGG	4 cut(s)	54, 73, 213, 303
MspR9I	CCNGG	4 cut(s)	54, 73, 336, 627
MvaI	CCWGG	2 cut(s)	336, 627
MwoI	GCNNNNNNNGC	2 cut(s)	467, 803
NciI	CCSGG	2 cut(s)	54, 73
NdeII	GATC	3 cut(s)	316, 504, 757
NlaIII	CATG	1 cut(s)	518
NlaIV	GGNNCC	4 cut(s)	211, 333, 379, 771
OliI	CACNNNNGTG	2 cut(s)	102, 386
PcsI	WCGNNNNNNNCGW	1 cut(s)	171
PfeI	GAWTC	3 cut(s)	578, 667, 816
PfoI	TCCNGGA	1 cut(s)	52
PinAI	ACCGGT	1 cut(s)	212
PkrI	GCNGC	5 cut(s)	244, 416, 472, 805, 871
PshBI	ATTAAT	2 cut(s)	438, 708
Psp6I	CCWGG	2 cut(s)	334, 625
PspGI	CCWGG	2 cut(s)	334, 625
PspN4I	GGNNCC	4 cut(s)	211, 333, 379, 771
PspPI	GGNCC	2 cut(s)	75, 378
PstNI	CAGNNNCTG	1 cut(s)	755
RsaI	GTAC	2 cut(s)	369, 554
RsaNI	GTAC	2 cut(s)	368, 553
RseI	CAYNNNNRTG	2 cut(s)	102, 386
SaqAI	TTAA	7 cut(s)	108, 180, 324, 438, 465, 708, 861
SatI	GCNGC	5 cut(s)	243, 415, 471, 804, 870
Sau3AI	GATC	3 cut(s)	316, 504, 757
Sau96I	GGNCC	2 cut(s)	75, 378
ScaI	AGTACT	1 cut(s)	369
ScrFI	CCNGG	4 cut(s)	54, 73, 336, 627
SexAI	ACCWGGT	1 cut(s)	625
SfaNI	GCATC	2 cut(s)	417, 761
SmiMI	CAYNNNNRTG	2 cut(s)	102, 386
Sse9I	AATT	3 cut(s)	148, 604, 858
SsiI	CCGC	5 cut(s)	69, 153, 245, 804, 872
StyD4I	CCNGG	4 cut(s)	52, 71, 334, 625
TaaI	ACNGT	3 cut(s)	104, 388, 735
TaiI	ACGT	1 cut(s)	168
TaqI	TCGA	1 cut(s)	786
TasI	AATT	3 cut(s)	148, 604, 858
TatI	WGTACW	1 cut(s)	367
TauI	GCSGC	1 cut(s)	806
TfiI	GAWTC	3 cut(s)	578, 667, 816
Tru1I	TTAA	7 cut(s)	108, 180, 324, 438, 465, 708, 861
Tru9I	TTAA	7 cut(s)	108, 180, 324, 438, 465, 708, 861
TscAI	CASTG	2 cut(s)	391, 516
TseI	GCWGC	4 cut(s)	242, 414, 470, 869
TspDTI	ATGAA	3 cut(s)	378, 534, 622
TspGWI	ACGGA	3 cut(s)	155, 498, 661
TspRI	CASTG	2 cut(s)	391, 516
VspI	ATTAAT	2 cut(s)	438, 708
ZrmI	AGTACT	1 cut(s)	369

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.