AT1G33440

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0013921
Physical Location & Seq
Reverse (-)
12127389 .. 12130444
3056 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G33440.1

Sequence Viewer

Length: 1806 bp
ATGGATGTTCATGATCTATCTGAAGAAGCCAAAAGAGGAGTAATACACACGTCGGAGGAATCATTGGACGATCTTTGTGTTGATTTTCGAGGTCGGCCATGCAGGCCAAGCAAGCATGGTGGTACAAGAGCTGCCCTCTTTGTTCTAGGCTTCCAAGCTTTCGAGATGATGGCGATCGCAGCTGTTGGAAACAACTTGATCACATACGTATTCAATGAGATGCATTTCCCTTTGTCAAAATCCGCAAACCTCGTTACTAACTTCATAGGAACGGTGTTTCTCCTCTCGCTTCTTGGTGGCTTCCTCTCTGATTCATACCTTGGAAGCTTTCGTACCATGCTCGTTTTTGGCGTCATCGAAATCTCTGGTTTCATATTATTGTCAGTCCAAGCTCATCTCCCAGAGCTAAGACCACCAGAATGCAACATGAAGAGCACCACAATACATTGCGTAGAGGCAAATGGTTACAAGGCAGCCACTCTCTACACCGCACTCTGTTTAGTGGCCTTAGGGAGCGGCTGCCTCAAACCGAACATCATCTCTCACGGTGCAAACCAGTTTCAAAGGAAAGATTTGAGGAAGCTCTCGAGTTTCTTCAATGCGGCTTACTTCGCCTTCTCAATGGGTCAGCTTATTGCACTCACACTCTTAGTTTGGGTGCAAACACATTCTGGTATGGACGTTGGTTTTGGTGTCTCCGCGGCTGTTATGGCCGCGGGGATGATCAGCCTTGTCGCTGGAACTAGCTTTTATAGGAATAAACCGCCTAGTGGTAGCATCTTCACACCAATTGCTCAGGTCTTCGTAGCCGCGATCACTAAACGAAAACAGATTTGCCCATCAAACCCAAACATGGTTCACCAACCTTCAACGGATCTTGTTCGTGTCAAACCTCTCCTCCATTCTAATAAATTCAGGTTTCTTGACAAGGCTTGCATCAAGACGCAAGGCAAGGCAATGGAGAGTCCATGGAGACTCTGCACAATCGAACAAGTCCATCAAGTCAAGATATTGTTATCAGTCATACCGATATTCGCGTGTACTATCATCTTCAACACCATCTTAGCTCAGCTTCAAACTTTCTCTGTTCAGCAAGGAAGTTCCATGAACACACACATTACAAAAACTTTCCAAATTCCACCAGCTTCTCTTCAAGCCATTCCTTACATCATTCTCATCTTCTTTGTGCCTCTCTACGAGACGTTCTTCGTCCCACTCGCTAGAAAACTCACGGGAAACGATTCTGGAATCTCTCCTCTTCAGAGAATTGGCACTGGACTGTTCTTAGCAACTTTCTCGATGGTTGCAGCCGCTTTAGTGGAGAAGAAGAGACGAGAATCGTTCTTGGAACAGAACGTAATGCTCTCGATCTTTTGGATCGCGCCGCAGTTTCTGATATTTGGTTTATCAGAGATGTTTACAGCCGTTGGATTAGTGGAGTTTTTTTACAAACAGTCTTCACAGAGTATGCAATCTTTTCTCACAGCAATGACATACTGTTCTTACTCTTTCGGGTTTTATCTTAGCTCTGTTCTTGTCTCGACCGTGAATAGAGTTACTTCTAGTAATGGTTCCGGGACCAAAGAAGGTTGGTTAGGCGATAATGACCTTAACAAAGATAGATTAGATCACTTTTACTGGCTTTTAGCTTCATTGAGTTTCATCAACTTCTTTAATTATCTTTTCTGGTCAAGATGGTATTCGTGTGATCCGTCCGCGACACATCATAGCGCCGAAGTCAATAGCTTGGAGGCTTTAGAAAATGGCGAAATCAAGGATTCCACGACGGAGAAGCCTAGAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009605 GO:0009607 GO:0009624 GO:0016020 GO:0043207 GO:0044464 GO:0050896 GO:0051704 GO:0051707 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

601

Amino Acids

66.7

Weight (kDa)

8.71

Isoelectric Point (pI)

41.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 110 - 526 7.9e-93 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013921)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33440
fragaria_vesca FvH4_2g00200
malus_domestica MD05G1000900.v1.1
prunus_persica Prupe.8G001900_v2.0.a1 Prupe.8G001900_v2.0.a1
pyrus_communis pycom05g00010
rosa_chinensis RchiOBHm_Chr6g0249481
rosa_laevigata RLG00000015577
rosa_multiflora Rmu_sc0002916.1_g000002
rosa_roxburghii Rroxscaffold_7G00217660
rosa_rugosa Rorug05G0492600 Rorug05G0492700
rosa_samantha Rh6AG002400 Rh6BG007700 Rh6CG001600 Rh6DG001600
rosa_wichuraiana Rw6G000300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 516
AccII CGCG 6 cut(s) 701, 716, 812, 1037, 1382, 1718
AclWI GGATC 3 cut(s) 882, 1385, 1703
AcoI YGGCCR 2 cut(s) 95, 711
AcsI RAATTY 3 cut(s) 911, 1134, 1800
AcuI CTGAAG 2 cut(s) 42, 1244
AcyI GRCGYC 1 cut(s) 351
AfaI GTAC 3 cut(s) 124, 334, 1042
AfiI CCNNNNNNNGG 2 cut(s) 770, 853
AflIII ACRYGT 1 cut(s) 48
AgsI TTSAA 7 cut(s) 214, 563, 598, 870, 1054, 1076, 1154
AjiI CACGTC 1 cut(s) 51
Alw21I GWGCWC 1 cut(s) 437
Alw26I GTCTC 5 cut(s) 700, 967, 1193, 1324, 1543
AlwI GGATC 3 cut(s) 882, 1385, 1703
AlwNI CAGNNNCTG 1 cut(s) 1393
Ama87I CYCGRG 1 cut(s) 586
AoxI GGCC 4 cut(s) 95, 104, 504, 711
ApeKI GCWGC 5 cut(s) 131, 179, 473, 519, 1307
ApoI RAATTY 3 cut(s) 911, 1134, 1800
ArsI GACNNNNNNTTYG 2 cut(s) 671, 703
AsiSI GCGATCGC 1 cut(s) 177
Asp700I GAANNNNTTC 1 cut(s) 1240
AspLEI GCGC 2 cut(s) 1384, 1733
AspS9I GGNCC 1 cut(s) 1578
AsuC2I CCSGG 1 cut(s) 1576
AsuHPI GGTGA 1 cut(s) 851
AvaI CYCGRG 1 cut(s) 586
AvaII GGWCC 1 cut(s) 1578
AxyI CCTNAGG 1 cut(s) 508
BarI GAAGNNNNNNTAC 2 cut(s) 316, 348
BbsI GAAGAC 2 cut(s) 793, 1449
Bbv12I GWGCWC 1 cut(s) 437
BbvI GCAGC 5 cut(s) 118, 191, 485, 506, 1319
BccI CCATC 6 cut(s) 163, 847, 1005, 1067, 1294, 1689
BceAI ACGGC 1 cut(s) 1409
BcgI CGANNNNNNTGC 2 cut(s) 1278, 1312
BclI TGATCA 2 cut(s) 198, 723
BcnI CCSGG 1 cut(s) 1576
BcoDI GTCTC 5 cut(s) 700, 967, 1193, 1324, 1543
BfaI CTAG 6 cut(s) 146, 744, 768, 1221, 1563, 1797
BfoI RGCGCY 1 cut(s) 1734
BglI GCCNNNNNGGC 1 cut(s) 103
BlpI GCTNAGC 1 cut(s) 1068
Bme1390I CCNGG 1 cut(s) 1576
Bme18I GGWCC 1 cut(s) 1578
BmeT110I CYCGRG 1 cut(s) 586
BmgBI CACGTC 1 cut(s) 51
BmgT120I GGNCC 1 cut(s) 1578
BmiI GGNNCC 2 cut(s) 1573, 1579
BmrFI CCNGG 1 cut(s) 1576
BmsI GCATC 3 cut(s) 210, 786, 945
BpiI GAAGAC 2 cut(s) 793, 1449
BplI GAGNNNNNCTC 2 cut(s) 120, 152
Bpu10I CCTNAGC 1 cut(s) 795
Bpu1102I GCTNAGC 1 cut(s) 1068
BpuMI CCSGG 1 cut(s) 1576
BsaAI YACGTR 1 cut(s) 208
BsaBI GATNNNNATC 1 cut(s) 173
BsaHI GRCGYC 1 cut(s) 351
BsaJI CCNNGG 4 cut(s) 319, 699, 714, 968
BsaXI ACNNNNNCTCC 2 cut(s) 882, 912
Bsc4I CCNNNNNNNGG 2 cut(s) 770, 853
Bse1I ACTGG 3 cut(s) 556, 1279, 1643
Bse21I CCTNAGG 1 cut(s) 508
Bse3DI GCAATG 3 cut(s) 445, 963, 1494
Bse8I GATNNNNATC 1 cut(s) 173
BseDI CCNNGG 4 cut(s) 319, 699, 714, 968
BseGI GGATG 2 cut(s) 10, 726
BseJI GATNNNNATC 1 cut(s) 173
BseLI CCNNNNNNNGG 2 cut(s) 770, 853
BseMI GCAATG 3 cut(s) 445, 963, 1494
BseMII CTCAG 2 cut(s) 809, 1082
BseNI ACTGG 3 cut(s) 556, 1279, 1643
BseRI GAGGAG 4 cut(s) 51, 272, 887, 1245
BseXI GCAGC 5 cut(s) 118, 191, 485, 506, 1319
BsgI GTGCAG 1 cut(s) 964
Bsh1236I CGCG 6 cut(s) 701, 716, 812, 1037, 1382, 1718
Bsh1285I CGRYCG 2 cut(s) 177, 1545
BshFI GGCC 4 cut(s) 97, 106, 506, 713
BsiEI CGRYCG 2 cut(s) 177, 1545
BsiHKAI GWGCWC 1 cut(s) 437
BsiHKCI CYCGRG 1 cut(s) 586
BsiSI CCGG 1 cut(s) 1575
BslFI GGGAC 2 cut(s) 1196, 1591
BslI CCNNNNNNNGG 2 cut(s) 770, 853
BsmAI GTCTC 5 cut(s) 700, 967, 1193, 1324, 1543
BsmBI CGTCTC 2 cut(s) 1193, 1324
BsmFI GGGAC 2 cut(s) 1196, 1591
BsmI GAATGC 1 cut(s) 425
BsnI GGCC 4 cut(s) 97, 106, 506, 713
BsoBI CYCGRG 1 cut(s) 586
Bsp1286I GDGCHC 1 cut(s) 437
Bsp1720I GCTNAGC 1 cut(s) 1068
Bsp19I CCATGG 1 cut(s) 968
BspANI GGCC 4 cut(s) 97, 106, 506, 713
BspCNI CTCAG 2 cut(s) 808, 1081
BspFNI CGCG 6 cut(s) 701, 716, 812, 1037, 1382, 1718
BspHI TCATGA 1 cut(s) 10
BspLI GGNNCC 2 cut(s) 1573, 1579
BspPI GGATC 3 cut(s) 882, 1385, 1703
BspQI GCTCTTC 1 cut(s) 425
BsrBI CCGCTC 1 cut(s) 516
BsrDI GCAATG 3 cut(s) 445, 963, 1494
BsrI ACTGG 3 cut(s) 556, 1279, 1643
BssECI CCNNGG 4 cut(s) 319, 699, 714, 968
BssNI GRCGYC 1 cut(s) 351
BssT1I CCWWGG 2 cut(s) 319, 968
Bst4CI ACNGT 6 cut(s) 274, 548, 1281, 1455, 1499, 1546
Bst6I CTCTTC 4 cut(s) 425, 1155, 1263, 1322
BstACI GRCGYC 1 cut(s) 351
BstBAI YACGTR 1 cut(s) 208
BstC8I GCNNGC 3 cut(s) 104, 113, 934
BstDEI CTNAG 8 cut(s) 407, 508, 649, 795, 1063, 1068, 1285, 1523
BstDSI CCRYGG 3 cut(s) 699, 714, 968
BstF5I GGATG 2 cut(s) 10, 726
BstFNI CGCG 6 cut(s) 701, 716, 812, 1037, 1382, 1718
BstH2I RGCGCY 1 cut(s) 1734
BstHHI GCGC 2 cut(s) 1384, 1733
BstMAI GTCTC 5 cut(s) 700, 967, 1193, 1324, 1543
BstMCI CGRYCG 2 cut(s) 177, 1545
BstMWI GCNNNNNNNGC 6 cut(s) 103, 108, 112, 179, 611, 710
BstSCI CCNGG 1 cut(s) 1574
BstSNI TACGTA 1 cut(s) 208
BstUI CGCG 6 cut(s) 701, 716, 812, 1037, 1382, 1718
BstV1I GCAGC 5 cut(s) 118, 191, 485, 506, 1319
BstV2I GAAGAC 2 cut(s) 793, 1449
BstX2I RGATCY 1 cut(s) 874
BstYI RGATCY 1 cut(s) 874
Bsu36I CCTNAGG 1 cut(s) 508
BsuRI GGCC 4 cut(s) 97, 106, 506, 713
BtgI CCRYGG 3 cut(s) 699, 714, 968
BtrI CACGTC 1 cut(s) 51
BtsCI GGATG 2 cut(s) 10, 726
BtsIMutI CAGTG 1 cut(s) 1272
Cac8I GCNNGC 3 cut(s) 104, 113, 934
CaiI CAGNNNCTG 1 cut(s) 1393
CciI TCATGA 1 cut(s) 10
CfoI GCGC 2 cut(s) 1384, 1733
Cfr13I GGNCC 1 cut(s) 1578
Cfr42I CCGCGG 2 cut(s) 702, 717
CseI GACGC 2 cut(s) 340, 952
Csp6I GTAC 3 cut(s) 123, 333, 1041
CspCI CAANNNNNGTGG 2 cut(s) 100, 135
CviAII CATG 8 cut(s) 11, 99, 116, 337, 427, 853, 969, 1105
CviQI GTAC 3 cut(s) 123, 333, 1041
DdeI CTNAG 8 cut(s) 407, 508, 649, 795, 1063, 1068, 1285, 1523
EaeI YGGCCR 2 cut(s) 95, 711
Eam1104I CTCTTC 4 cut(s) 425, 1155, 1263, 1322
EarI CTCTTC 4 cut(s) 425, 1155, 1263, 1322
Eco105I TACGTA 1 cut(s) 208
Eco130I CCWWGG 2 cut(s) 319, 968
Eco47I GGWCC 1 cut(s) 1578
Eco57I CTGAAG 2 cut(s) 42, 1244
Eco81I CCTNAGG 1 cut(s) 508
Eco88I CYCGRG 1 cut(s) 586
EcoT14I CCWWGG 2 cut(s) 319, 968
EcoT22I ATGCAT 1 cut(s) 225
ErhI CCWWGG 2 cut(s) 319, 968
Esp3I CGTCTC 2 cut(s) 1193, 1324
FaeI CATG 8 cut(s) 14, 102, 119, 340, 430, 856, 972, 1108
FaqI GGGAC 2 cut(s) 1196, 1591
FatI CATG 8 cut(s) 10, 98, 115, 336, 426, 852, 968, 1104
FauI CCCGC 1 cut(s) 709
FbaI TGATCA 2 cut(s) 198, 723
FokI GGATG 2 cut(s) 17, 733
FspBI CTAG 6 cut(s) 146, 744, 768, 1221, 1563, 1797
GlaI GCGC 2 cut(s) 1383, 1732
HaeII RGCGCY 1 cut(s) 1734
HaeIII GGCC 4 cut(s) 97, 106, 506, 713
HapII CCGG 1 cut(s) 1575
HgaI GACGC 2 cut(s) 340, 952
HhaI GCGC 2 cut(s) 1384, 1733
Hin1I GRCGYC 1 cut(s) 351
Hin1II CATG 8 cut(s) 14, 102, 119, 340, 430, 856, 972, 1108
Hin6I GCGC 2 cut(s) 1382, 1731
HinP1I GCGC 2 cut(s) 1382, 1731
HindIII AAGCTT 2 cut(s) 156, 325
HinfI GANTC 8 cut(s) 59, 311, 964, 975, 1241, 1248, 1337, 1778
HpaII CCGG 1 cut(s) 1575
HphI GGTGA 1 cut(s) 851
Hpy166II GTNNAC 3 cut(s) 859, 1041, 1419
Hpy188I TCNGA 6 cut(s) 22, 55, 310, 1263, 1395, 1411
Hpy8I GTNNAC 3 cut(s) 859, 1041, 1419
Hpy99I CGWCG 2 cut(s) 55, 1789
HpyAV CCTTC 3 cut(s) 625, 876, 1580
HpyCH4III ACNGT 6 cut(s) 274, 548, 1281, 1455, 1499, 1546
HpyCH4IV ACGT 5 cut(s) 50, 207, 681, 1202, 1356
HpyF10VI GCNNNNNNNGC 6 cut(s) 103, 108, 112, 179, 611, 710
HpyF3I CTNAG 8 cut(s) 407, 508, 649, 795, 1063, 1068, 1285, 1523
HpySE526I ACGT 5 cut(s) 50, 207, 681, 1202, 1356
Hsp92I GRCGYC 1 cut(s) 351
Hsp92II CATG 8 cut(s) 14, 102, 119, 340, 430, 856, 972, 1108
HspAI GCGC 2 cut(s) 1382, 1731
Ksp22I TGATCA 2 cut(s) 198, 723
KspI CCGCGG 2 cut(s) 702, 717
LguI GCTCTTC 1 cut(s) 425
LmnI GCTCC 1 cut(s) 513
Lsp1109I GCAGC 5 cut(s) 118, 191, 485, 506, 1319
LweI GCATC 3 cut(s) 210, 786, 945
MaeI CTAG 6 cut(s) 146, 744, 768, 1221, 1563, 1797
MaeII ACGT 5 cut(s) 50, 207, 681, 1202, 1356
MaeIII GTNAC 3 cut(s) 253, 464, 1555
MbiI CCGCTC 1 cut(s) 516
MfeI CAATTG 1 cut(s) 789
MflI RGATCY 1 cut(s) 874
MhlI GDGCHC 1 cut(s) 437
MluCI AATT 6 cut(s) 789, 911, 1134, 1266, 1675, 1800
MlyI GAGTC 2 cut(s) 969, 973
MmeI TCCRAC 3 cut(s) 33, 166, 1408
Mph1103I ATGCAT 1 cut(s) 225
MroXI GAANNNNTTC 1 cut(s) 1240
MseI TTAA 2 cut(s) 1611, 1674
MslI CAYNNNNRTG 2 cut(s) 418, 1487
MspA1I CMGCKG 3 cut(s) 182, 701, 716
MspI CCGG 1 cut(s) 1575
MspR9I CCNGG 1 cut(s) 1576
MunI CAATTG 1 cut(s) 789
Mva1269I GAATGC 1 cut(s) 425
MvnI CGCG 6 cut(s) 701, 716, 812, 1037, 1382, 1718
MwoI GCNNNNNNNGC 6 cut(s) 103, 108, 112, 179, 611, 710
NciI CCSGG 1 cut(s) 1576
NcoI CCATGG 1 cut(s) 968
NlaIII CATG 8 cut(s) 14, 102, 119, 340, 430, 856, 972, 1108
NlaIV GGNNCC 2 cut(s) 1573, 1579
NsiI ATGCAT 1 cut(s) 225
PaeR7I CTCGAG 1 cut(s) 586
PagI TCATGA 1 cut(s) 10
PciSI GCTCTTC 1 cut(s) 425
PcsI WCGNNNNNNNCGW 2 cut(s) 348, 1709
PctI GAATGC 1 cut(s) 425
PdmI GAANNNNTTC 1 cut(s) 1240
PfeI GAWTC 6 cut(s) 59, 311, 1241, 1248, 1337, 1778
PfoI TCCNGGA 1 cut(s) 1574
Ple19I CGATCG 1 cut(s) 177
PleI GAGTC 2 cut(s) 969, 972
PpsI GAGTC 2 cut(s) 969, 972
Ppu21I YACGTR 1 cut(s) 208
PspN4I GGNNCC 2 cut(s) 1573, 1579
PspPI GGNCC 1 cut(s) 1578
PstNI CAGNNNCTG 1 cut(s) 1393
PsuI RGATCY 1 cut(s) 874
PvuI CGATCG 1 cut(s) 177
PvuII CAGCTG 1 cut(s) 182
RgaI GCGATCGC 1 cut(s) 177
RsaI GTAC 3 cut(s) 124, 334, 1042
RsaNI GTAC 3 cut(s) 123, 333, 1041
RseI CAYNNNNRTG 2 cut(s) 418, 1487
SacII CCGCGG 2 cut(s) 702, 717
SapI GCTCTTC 1 cut(s) 425
SaqAI TTAA 2 cut(s) 1611, 1674
Sau96I GGNCC 1 cut(s) 1578
SchI GAGTC 2 cut(s) 969, 973
ScrFI CCNGG 1 cut(s) 1576
SduI GDGCHC 1 cut(s) 437
SfaAI GCGATCGC 1 cut(s) 177
SfaNI GCATC 3 cut(s) 210, 786, 945
SfiI GGCCNNNNNGGCC 1 cut(s) 103
Sfr274I CTCGAG 1 cut(s) 586
Sfr303I CCGCGG 2 cut(s) 702, 717
SgfI GCGATCGC 1 cut(s) 177
SgrBI CCGCGG 2 cut(s) 702, 717
SinI GGWCC 1 cut(s) 1578
SlaI CTCGAG 1 cut(s) 586
SmiMI CAYNNNNRTG 2 cut(s) 418, 1487
SmlI CTYRAG 1 cut(s) 586
SmoI CTYRAG 1 cut(s) 586
SnaBI TACGTA 1 cut(s) 208
Sse9I AATT 6 cut(s) 789, 911, 1134, 1266, 1675, 1800
SspMI CTAG 6 cut(s) 146, 744, 768, 1221, 1563, 1797
StyD4I CCNGG 1 cut(s) 1574
StyI CCWWGG 2 cut(s) 319, 968
TaaI ACNGT 6 cut(s) 274, 548, 1281, 1455, 1499, 1546
TaiI ACGT 5 cut(s) 53, 210, 684, 1205, 1359
TaqI TCGA 8 cut(s) 88, 162, 357, 587, 987, 1298, 1367, 1541
TasI AATT 6 cut(s) 789, 911, 1134, 1266, 1675, 1800
TatI WGTACW 1 cut(s) 1040
TauI GCSGC 7 cut(s) 519, 605, 704, 716, 812, 1313, 1387
TfiI GAWTC 6 cut(s) 59, 311, 1241, 1248, 1337, 1778
Tru1I TTAA 2 cut(s) 1611, 1674
Tru9I TTAA 2 cut(s) 1611, 1674
TscAI CASTG 1 cut(s) 1279
TseI GCWGC 5 cut(s) 131, 179, 473, 519, 1307
TspDTI ATGAA 7 cut(s) 253, 303, 361, 443, 1121, 1641, 1651
TspGWI ACGGA 3 cut(s) 887, 1701, 1802
TspRI CASTG 1 cut(s) 1279
VpaK11BI GGWCC 1 cut(s) 1578
XapI RAATTY 3 cut(s) 911, 1134, 1800
XhoI CTCGAG 1 cut(s) 586
XmnI GAANNNNTTC 1 cut(s) 1240
XspI CTAG 6 cut(s) 146, 744, 768, 1221, 1563, 1797
Zsp2I ATGCAT 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.