FvH4_2g00200

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0013921
Physical Location & Seq
Forward (+)
293067 .. 297269
4203 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g00200.t1

Sequence Viewer

Length: 1827 bp
ATGTCCATCTCTGAGGCTGCAGAAGCGGTGGAGGATATCTCTGTTGATTGGAGAGGCAGACCCTGCTGTAAGCCAACCAAACATGGTGGAATGAAGGCTGCTGTTTTTGTCCTAGGTCTCCAAGGTTTTGAGATGATGGCAATAGCAGCTGTTGGGAACAATCTCATCACCTATGTACTAAATGAGATGCACTTTCCTCTGTCAAAGTCTGCCAACATAGTCACCAACTTTATAGGGACAGTCTTCCTCCTCTCCCTCTTTGGTGGGTTCCTATCAGATTCTTATCTTGGGAGCTTCTGGACCATGCTCATCTTTGGCTTTGTGGAGCTTTCTGGTTTCATACTACTCTCGGTTCAAGCCCATCTCCCCCAACTAAGGCCTCCCTCCTGCAAGGTTATGTCGTCCGAGTACTGTCAGGAAGCAAAGGGATACAAGGCTCTCATATTCTTTGCTGCAATTTACTTGGTAGCCTTAGGAAGTGGTTGTCTAAAACCAAACATAATCTCTCATGGAGCTGACCAGTTCAGGAAGGAGGATTCCAAGCAATCCAAGAAGTTATCGACTTACTTCAATTGCGCATACTTTTCCTTCTGCATGGGAGAACTCATTGCATTAACAGTCCTTGTTTGGGTCCAAACACACTCCGGCATGGATGTTGGATTTGGAGTCTCTGCTGCTGCGATGACACTCGGACTAATTAGCCTGGTGTCCGGTACGTCTATGTATCGGAACAAACCACCTCGCGGAAGCATTTTCACTCCCATTGCTCAAGTTTTCGTTGCTGCATTTACAAAGAGAAAGCAAATATGCCCTTCAAATACAGAAATGCTTCATGGAAACCGAAACAATGTGCCAAACCACCTCTTTGTCACCATGTCGCCTAACGTCACCACTCTCCTCCACACCGAAAAGTTCAGATTTCTGGACAAGGCATGCATCAAAACAGAAGATGGAACTGAGAGGACCGAAAGTCCATGGAGATTATGCACCGTGTCTCAGGTGGAGCAAGTCAAAATAATTATCTCAGTTGTGCCCATTTTCGCCTGTACCATCATCTTCAACACAATTTTGGCTCAACTCCAAACATTTTCAGTCCAACAAGGAAGCACAATGAACGCAAGGATCACAGGAAGCTTCAAAATCCCCCCAGCTTCACTCCAAGCCATCCCTTACATCATGCTAATCTTTCTAGTCCCTCTATACGAAACAGTCTTCGTACCCACTGCTCGGAAAGTCACCGGAAGGGACTCTGGGATTTCTCCTTTACAAAGAGTTGGCACTGGCCTTTTTATTGCCACCTTTTCAATGGTTTCAGCTGCAGTTATTGAGAGAAAGAGAAGAAACTCGGCTGAAGCTCTTTCGATCTTTTGGATTGCTCCACAGTTTCTCATCTTCGGACTCTCAGAGATGTTCACTGCAGTAGGGCTTATAGAGTTCTTCTACAAGCAGTCATTGGAAGGAATGCAATCATTTCTGACTGCCATGACATACTGCTCGTACTCATTTGGCTTCTATTTGAGCTCGCTCCTTGTTTCTCTTGTGAACAAAGTGACCTCAACTTCTTCGAGGGGAGGGTGGCTCAGCGACAATGATCTCAACAAGGATAAGCTGGACCGTTTCTATTGGCTGCTGGCTGCTCTCAGCCTCATCAACTTCTTCAATTATCTCTTCTGGTCAAATTGGTATCGTTACAACCCCTCTTTATTAGCACCGGCCACTAATACTACTAATTCACCCCATCAAGCTGAAGATGGAAAAGTAGTCGACTTGGAAAATAACATGCAGCAGCAGCAGCAGCAACTTCAAGTATCCTTCAAGCCATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

609

Amino Acids

67.64

Weight (kDa)

8.92

Isoelectric Point (pI)

39.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 99 - 526 9.9e-93 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013921)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33440
fragaria_vesca FvH4_2g00200
malus_domestica MD05G1000900.v1.1
prunus_persica Prupe.8G001900_v2.0.a1 Prupe.8G001900_v2.0.a1
pyrus_communis pycom05g00010
rosa_chinensis RchiOBHm_Chr6g0249481
rosa_laevigata RLG00000015577
rosa_multiflora Rmu_sc0002916.1_g000002
rosa_roxburghii Rroxscaffold_7G00217660
rosa_rugosa Rorug05G0492600 Rorug05G0492700
rosa_samantha Rh6AG002400 Rh6BG007700 Rh6CG001600 Rh6DG001600
rosa_wichuraiana Rw6G000300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 577
AccI GTMKAC 1 cut(s) 1764
AccII CGCG 1 cut(s) 744
AciI CCGC 2 cut(s) 26, 744
AclWI GGATC 1 cut(s) 1130
AcoI YGGCCR 1 cut(s) 1713
AcuI CTGAAG 2 cut(s) 1371, 1767
AfaI GTAC 6 cut(s) 177, 410, 715, 1048, 1218, 1499
AfiI CCNNNNNNNGG 4 cut(s) 375, 628, 743, 1227
AgsI TTSAA 9 cut(s) 356, 571, 816, 1060, 1138, 1305, 1660, 1805, 1816
AhdI GACNNNNNGTC 1 cut(s) 969
AjnI CCWGG 1 cut(s) 702
Alw21I GWGCWC 1 cut(s) 1523
Alw26I GTCTC 3 cut(s) 122, 673, 999
AlwI GGATC 1 cut(s) 1130
AlwNI CAGNNNCTG 1 cut(s) 63
AoxI GGCC 3 cut(s) 377, 1282, 1713
Asp700I GAANNNNTTC 1 cut(s) 828
AspA2I CCTAGG 1 cut(s) 112
AspLEI GCGC 1 cut(s) 578
AspS9I GGNCC 4 cut(s) 300, 631, 963, 1612
AsuHPI GGTGA 6 cut(s) 160, 214, 862, 880, 1228, 1725
AvaII GGWCC 4 cut(s) 300, 631, 963, 1612
AvrII CCTAGG 1 cut(s) 112
AxyI CCTNAGG 1 cut(s) 472
BaeGI GKGCMC 1 cut(s) 1035
BanII GRGCYC 1 cut(s) 1523
BbsI GAAGAC 2 cut(s) 235, 1204
Bbv12I GWGCWC 1 cut(s) 1523
BccI CCATC 8 cut(s) 14, 130, 369, 944, 1058, 1172, 1745, 1746
BciT130I CCWGG 1 cut(s) 704
BciVI GTATCC 2 cut(s) 422, 1819
BcoDI GTCTC 3 cut(s) 122, 673, 999
BfaI CTAG 2 cut(s) 113, 1190
BfmI CTRYAG 3 cut(s) 18, 1317, 1416
BfuI GTATCC 2 cut(s) 422, 1819
BlnI CCTAGG 1 cut(s) 112
BlpI GCTNAGC 1 cut(s) 1580
BmcAI AGTACT 1 cut(s) 410
Bme1390I CCNGG 1 cut(s) 704
Bme18I GGWCC 4 cut(s) 300, 631, 963, 1612
BmeRI GACNNNNNGTC 1 cut(s) 969
BmgT120I GGNCC 4 cut(s) 300, 631, 963, 1612
BmiI GGNNCC 2 cut(s) 269, 632
BmrFI CCNGG 1 cut(s) 704
BmsI GCATC 2 cut(s) 177, 945
BpiI GAAGAC 2 cut(s) 235, 1204
BplI GAGNNNNNCTC 4 cut(s) 23, 55, 1563, 1595
Bpu1102I GCTNAGC 1 cut(s) 1580
BpuEI CTTGAG 1 cut(s) 753
BsaBI GATNNNNATC 1 cut(s) 282
BsaI GGTCTC 1 cut(s) 122
BsaJI CCNNGG 3 cut(s) 112, 121, 974
BsaWI WCCGGW 2 cut(s) 710, 1238
Bsc4I CCNNNNNNNGG 4 cut(s) 375, 628, 743, 1227
Bse118I RCCGGY 1 cut(s) 1711
Bse1I ACTGG 2 cut(s) 520, 1285
Bse21I CCTNAGG 1 cut(s) 472
Bse3DI GCAATG 2 cut(s) 606, 762
Bse8I GATNNNNATC 1 cut(s) 282
BseBI CCWGG 1 cut(s) 704
BseDI CCNNGG 3 cut(s) 112, 121, 974
BseGI GGATG 2 cut(s) 658, 1164
BseJI GATNNNNATC 1 cut(s) 282
BseLI CCNNNNNNNGG 4 cut(s) 375, 628, 743, 1227
BseMI GCAATG 2 cut(s) 606, 762
BseMII CTCAG 6 cut(s) 948, 1010, 1038, 1416, 1594, 1654
BseNI ACTGG 2 cut(s) 520, 1285
BseRI GAGGAG 2 cut(s) 239, 887
BseSI GKGCMC 1 cut(s) 1035
BseYI CCCAGC 1 cut(s) 1147
Bsh1236I CGCG 1 cut(s) 744
BshFI GGCC 3 cut(s) 379, 1284, 1715
BsiHKAI GWGCWC 1 cut(s) 1523
BsiSI CCGG 4 cut(s) 645, 711, 1239, 1712
BslFI GGGAC 3 cut(s) 250, 1178, 1259
BslI CCNNNNNNNGG 4 cut(s) 375, 628, 743, 1227
BsmAI GTCTC 3 cut(s) 122, 673, 999
BsmFI GGGAC 3 cut(s) 250, 1178, 1259
BsmI GAATGC 1 cut(s) 1467
BsnI GGCC 3 cut(s) 379, 1284, 1715
Bso31I GGTCTC 1 cut(s) 122
Bsp1286I GDGCHC 2 cut(s) 1035, 1523
Bsp143I GATC 3 cut(s) 1122, 1362, 1591
Bsp1720I GCTNAGC 1 cut(s) 1580
Bsp19I CCATGG 1 cut(s) 974
BspACI CCGC 2 cut(s) 26, 744
BspANI GGCC 3 cut(s) 379, 1284, 1715
BspCNI CTCAG 7 cut(s) 4, 949, 1009, 1037, 1415, 1593, 1653
BspFNI CGCG 1 cut(s) 744
BspLI GGNNCC 2 cut(s) 269, 632
BspMAI CTGCAG 3 cut(s) 22, 1321, 1420
BspPI GGATC 1 cut(s) 1130
BspTNI GGTCTC 1 cut(s) 122
BsrDI GCAATG 2 cut(s) 606, 762
BsrFI RCCGGY 1 cut(s) 1711
BsrI ACTGG 2 cut(s) 520, 1285
BssAI RCCGGY 1 cut(s) 1711
BssECI CCNNGG 3 cut(s) 112, 121, 974
BssMI GATC 3 cut(s) 1122, 1362, 1591
BssT1I CCWWGG 3 cut(s) 112, 121, 974
Bst2UI CCWGG 1 cut(s) 704
Bst4CI ACNGT 7 cut(s) 241, 413, 619, 991, 1210, 1383, 1616
Bst6I CTCTTC 1 cut(s) 1673
BstAPI GCANNNNNTGC 1 cut(s) 63
BstC8I GCNNGC 3 cut(s) 934, 1523, 1632
BstDEI CTNAG 9 cut(s) 12, 374, 472, 957, 996, 1024, 1402, 1580, 1640
BstDSI CCRYGG 1 cut(s) 974
BstF5I GGATG 2 cut(s) 658, 1164
BstFNI CGCG 1 cut(s) 744
BstHHI GCGC 1 cut(s) 578
BstKTI GATC 3 cut(s) 1125, 1365, 1594
BstMAI GTCTC 3 cut(s) 122, 673, 999
BstMBI GATC 3 cut(s) 1122, 1362, 1591
BstMWI GCNNNNNNNGC 6 cut(s) 23, 63, 146, 1789, 1792, 1795
BstNI CCWGG 1 cut(s) 704
BstNSI RCATGY 2 cut(s) 936, 1783
BstSCI CCNGG 1 cut(s) 702
BstSFI CTRYAG 3 cut(s) 18, 1317, 1416
BstSLI GKGCMC 1 cut(s) 1035
BstUI CGCG 1 cut(s) 744
BstV2I GAAGAC 2 cut(s) 235, 1204
Bsu36I CCTNAGG 1 cut(s) 472
BsuI GTATCC 2 cut(s) 422, 1819
BsuRI GGCC 3 cut(s) 379, 1284, 1715
BtgI CCRYGG 1 cut(s) 974
BtgZI GCGATG 1 cut(s) 695
BtsCI GGATG 2 cut(s) 658, 1164
BtsI GCAGTG 2 cut(s) 1221, 1413
BtsIMutI CAGTG 3 cut(s) 1221, 1278, 1413
Cac8I GCNNGC 3 cut(s) 934, 1523, 1632
CaiI CAGNNNCTG 1 cut(s) 63
CfoI GCGC 1 cut(s) 578
Cfr10I RCCGGY 1 cut(s) 1711
Cfr13I GGNCC 4 cut(s) 300, 631, 963, 1612
Csp6I GTAC 6 cut(s) 176, 409, 714, 1047, 1217, 1498
CspCI CAANNNNNGTGG 2 cut(s) 67, 102
CviQI GTAC 6 cut(s) 176, 409, 714, 1047, 1217, 1498
DdeI CTNAG 9 cut(s) 12, 374, 472, 957, 996, 1024, 1402, 1580, 1640
DpnI GATC 3 cut(s) 1124, 1364, 1593
DpnII GATC 3 cut(s) 1122, 1362, 1591
DriI GACNNNNNGTC 1 cut(s) 969
EaeI YGGCCR 1 cut(s) 1713
Eam1104I CTCTTC 1 cut(s) 1673
Eam1105I GACNNNNNGTC 1 cut(s) 969
EarI CTCTTC 1 cut(s) 1673
Ecl136II GAGCTC 1 cut(s) 1521
Eco130I CCWWGG 3 cut(s) 112, 121, 974
Eco147I AGGCCT 1 cut(s) 379
Eco24I GRGCYC 1 cut(s) 1523
Eco31I GGTCTC 1 cut(s) 122
Eco32I GATATC 1 cut(s) 37
Eco47I GGWCC 4 cut(s) 300, 631, 963, 1612
Eco53kI GAGCTC 1 cut(s) 1521
Eco57I CTGAAG 2 cut(s) 1371, 1767
Eco81I CCTNAGG 1 cut(s) 472
EcoICRI GAGCTC 1 cut(s) 1521
EcoRII CCWGG 1 cut(s) 702
EcoRV GATATC 1 cut(s) 37
EcoT14I CCWWGG 3 cut(s) 112, 121, 974
EcoT22I ATGCAT 1 cut(s) 938
EcoT38I GRGCYC 1 cut(s) 1523
ErhI CCWWGG 3 cut(s) 112, 121, 974
FaqI GGGAC 3 cut(s) 250, 1178, 1259
FblI GTMKAC 1 cut(s) 1764
FokI GGATG 2 cut(s) 665, 1151
FriOI GRGCYC 1 cut(s) 1523
FspBI CTAG 2 cut(s) 113, 1190
FspI TGCGCA 1 cut(s) 577
GlaI GCGC 1 cut(s) 577
GsaI CCCAGC 1 cut(s) 1151
HaeIII GGCC 3 cut(s) 379, 1284, 1715
HapII CCGG 4 cut(s) 645, 711, 1239, 1712
HhaI GCGC 1 cut(s) 578
Hin6I GCGC 1 cut(s) 576
HinP1I GCGC 1 cut(s) 576
HincII GTYRAC 1 cut(s) 1765
HindII GTYRAC 1 cut(s) 1765
HindIII AAGCTT 1 cut(s) 1132
HinfI GANTC 5 cut(s) 278, 536, 666, 1247, 1398
HpaII CCGG 4 cut(s) 645, 711, 1239, 1712
HphI GGTGA 6 cut(s) 160, 214, 862, 880, 1228, 1725
Hpy166II GTNNAC 3 cut(s) 1413, 1543, 1765
Hpy188III TCNNGA 4 cut(s) 298, 416, 526, 923
Hpy8I GTNNAC 3 cut(s) 1413, 1543, 1765
HpyAV CCTTC 7 cut(s) 88, 523, 598, 822, 1236, 1451, 1822
HpyCH4III ACNGT 7 cut(s) 241, 413, 619, 991, 1210, 1383, 1616
HpyCH4IV ACGT 2 cut(s) 716, 885
HpyF10VI GCNNNNNNNGC 6 cut(s) 23, 63, 146, 1789, 1792, 1795
HpyF3I CTNAG 9 cut(s) 12, 374, 472, 957, 996, 1024, 1402, 1580, 1640
HpySE526I ACGT 2 cut(s) 716, 885
HspAI GCGC 1 cut(s) 576
Kzo9I GATC 3 cut(s) 1122, 1362, 1591
LmnI GCTCC 6 cut(s) 291, 325, 512, 1003, 1381, 1530
LweI GCATC 2 cut(s) 177, 945
MaeI CTAG 2 cut(s) 113, 1190
MaeII ACGT 2 cut(s) 716, 885
MaeIII GTNAC 6 cut(s) 220, 868, 886, 1234, 1549, 1688
MalI GATC 3 cut(s) 1124, 1364, 1593
MboI GATC 3 cut(s) 1122, 1362, 1591
MfeI CAATTG 1 cut(s) 571
MhlI GDGCHC 2 cut(s) 1035, 1523
MluCI AATT 8 cut(s) 456, 571, 696, 1017, 1065, 1660, 1678, 1729
MlyI GAGTC 3 cut(s) 675, 1241, 1392
MmeI TCCRAC 2 cut(s) 637, 1120
Mph1103I ATGCAT 1 cut(s) 938
MroXI GAANNNNTTC 1 cut(s) 828
MseI TTAA 1 cut(s) 614
MspA1I CMGCKG 2 cut(s) 149, 1316
MspI CCGG 4 cut(s) 645, 711, 1239, 1712
MspR9I CCNGG 1 cut(s) 704
MunI CAATTG 1 cut(s) 571
Mva1269I GAATGC 1 cut(s) 1467
MvaI CCWGG 1 cut(s) 704
MvnI CGCG 1 cut(s) 744
MwoI GCNNNNNNNGC 6 cut(s) 23, 63, 146, 1789, 1792, 1795
NcoI CCATGG 1 cut(s) 974
NdeII GATC 3 cut(s) 1122, 1362, 1591
NlaIV GGNNCC 2 cut(s) 269, 632
NmeAIII GCCGAG 1 cut(s) 1325
NmuCI GTSAC 5 cut(s) 220, 868, 886, 1234, 1549
NsbI TGCGCA 1 cut(s) 577
NsiI ATGCAT 1 cut(s) 938
NspI RCATGY 2 cut(s) 936, 1783
PaeI GCATGC 1 cut(s) 936
PceI AGGCCT 1 cut(s) 379
PctI GAATGC 1 cut(s) 1467
PdmI GAANNNNTTC 1 cut(s) 828
PfeI GAWTC 2 cut(s) 278, 536
PleI GAGTC 3 cut(s) 674, 1241, 1392
PpsI GAGTC 3 cut(s) 674, 1241, 1392
Psp124BI GAGCTC 1 cut(s) 1523
Psp6I CCWGG 1 cut(s) 702
PspFI CCCAGC 1 cut(s) 1147
PspGI CCWGG 1 cut(s) 702
PspN4I GGNNCC 2 cut(s) 269, 632
PspPI GGNCC 4 cut(s) 300, 631, 963, 1612
PstI CTGCAG 3 cut(s) 22, 1321, 1420
PstNI CAGNNNCTG 1 cut(s) 63
PvuII CAGCTG 2 cut(s) 149, 1316
RsaI GTAC 6 cut(s) 177, 410, 715, 1048, 1218, 1499
RsaNI GTAC 6 cut(s) 176, 409, 714, 1047, 1217, 1498
SacI GAGCTC 1 cut(s) 1523
SalI GTCGAC 1 cut(s) 1763
SaqAI TTAA 1 cut(s) 614
Sau3AI GATC 3 cut(s) 1122, 1362, 1591
Sau96I GGNCC 4 cut(s) 300, 631, 963, 1612
ScaI AGTACT 1 cut(s) 410
SchI GAGTC 3 cut(s) 675, 1241, 1392
ScrFI CCNGG 1 cut(s) 704
SduI GDGCHC 2 cut(s) 1035, 1523
SfaNI GCATC 2 cut(s) 177, 945
SfcI CTRYAG 3 cut(s) 18, 1317, 1416
SinI GGWCC 4 cut(s) 300, 631, 963, 1612
SmlI CTYRAG 1 cut(s) 768
SmoI CTYRAG 1 cut(s) 768
SphI GCATGC 1 cut(s) 936
Sse9I AATT 8 cut(s) 456, 571, 696, 1017, 1065, 1660, 1678, 1729
SseBI AGGCCT 1 cut(s) 379
SsiI CCGC 2 cut(s) 26, 744
SspMI CTAG 2 cut(s) 113, 1190
SstI GAGCTC 1 cut(s) 1523
StuI AGGCCT 1 cut(s) 379
StyD4I CCNGG 1 cut(s) 702
StyI CCWWGG 3 cut(s) 112, 121, 974
TaaI ACNGT 7 cut(s) 241, 413, 619, 991, 1210, 1383, 1616
TaiI ACGT 2 cut(s) 719, 888
TaqI TCGA 4 cut(s) 560, 1361, 1565, 1764
TaqII GACCGA 1 cut(s) 980
TasI AATT 8 cut(s) 456, 571, 696, 1017, 1065, 1660, 1678, 1729
TatI WGTACW 2 cut(s) 175, 408
TfiI GAWTC 2 cut(s) 278, 536
Tru1I TTAA 1 cut(s) 614
Tru9I TTAA 1 cut(s) 614
TscAI CASTG 3 cut(s) 1228, 1285, 1420
TseFI GTSAC 5 cut(s) 220, 868, 886, 1234, 1549
Tsp45I GTSAC 5 cut(s) 220, 868, 886, 1234, 1549
TspDTI ATGAA 4 cut(s) 107, 328, 821, 1127
TspRI CASTG 3 cut(s) 1228, 1285, 1420
VpaK11BI GGWCC 4 cut(s) 300, 631, 963, 1612
XceI RCATGY 2 cut(s) 936, 1783
XcmI CCANNNNNNNNNTGG 1 cut(s) 1303
XmaJI CCTAGG 1 cut(s) 112
XmiI GTMKAC 1 cut(s) 1764
XmnI GAANNNNTTC 1 cut(s) 828
XspI CTAG 2 cut(s) 113, 1190
ZrmI AGTACT 1 cut(s) 410
Zsp2I ATGCAT 1 cut(s) 938
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.