pycom05g00010

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0013921
Physical Location & Seq
Forward (+)
28845 .. 33187
4343 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g00010.3

Sequence Viewer

Length: 1914 bp
ATGAAATTAAGAGGAGATTCAAAGAGTGAGGGTGCAGTGGTGGATTCGGAGGATATGTCTGTTGATTGGAGAGGCAGACCCTGCAAGCCTACCAACCATGGTGGAATGAGGGCTGCTGTTTTTGTCCTTGGTCTGCAGGGTTTTGAGATGATGGCAATAGCAGGTGTTGGGAACAATCTGATTACGTATGTACTAAATGAGATGCACTTTTCACTGTCAAAGTCTGCAAACATAGTCACGAACTTCATAGGGACAGTCTTCCTCCTCTCCCTCCTTGGTGGCTACCTTTCTGATTCATACCTTGGGAGCTTCTGGACCATGATCATCTTCGGCTTTGTCGAGCTCTCTGGTTTTATATTGCTGTGCGTGCAAGCCCATCTCCCACAATTAAGGCCACCCTCCTGCAACAATATTATGTCATCCCATGAGCATGAGGAGGAGTTATGTCCTGAGGCAAAGGGCTACAAGGCCCTGATATTCTTTGCTGCAATATACCTGGTGGCCTTAGGAAGCGGTTGCCTAAAACCGAACATAATCTCTCATGGGGCCGACCAGTTCAGGAGGGACGACTCCAAGCAGTCTAAGAAGCTATCATCTTACTTCAACTGCGCTTACTTCGCCTTCTGCACCGGAGAACTCATTGCACTAACAGTCCTTGTTTGGGTCCAAACACACTCCGGGATGGATGTTGGGTTTGGAGTCTCAGCAGCTGCCATGGCAATGGGATTATTCTGCTTGACTTTTGGTACATCTCTTTACAGGAACAAACCCCCTCGAGGAAGTATTTTCACTCCTATTGCTCAAGTAATTAACCAACCTGATGATCTCACTAATTGCCTCCCTTCTCCCTGCTTCTCTAACTTCTCCAATGTTTTTGTTGCTGCGTTTACAAAGAGAAAGCAAATCTGTCCTTCAAATACAGAAATGCTGCATGGAAACCGAAACAGTGTGCCAATGTCAAACCACCTCTTTGTTCCCATGTCACCAAACGTCACTACTCTCCTCCACACTGAAAAGTTCAGATTCCTGGACAAGGCATGCATCAAAATTGAAGATGGAAGTCAGCGGATCGAAAGTTCTTGGAGATTATGCACCGTCGCACAGGTGGAGCAAGTCAAAATCATTTTCTCAGTTGTGCCCATTTTTGCATGTACCATCATCTTCAACACAATTTTAGCTCAACTCCAGACATTCTCCGTCCAGCAAGGAAGTTCCATGAACACTAGGATCGCGAAAGGTTTCAAAGTTCCCCCAGCTTCGCTTCAAGCCATCCCTTACATTATGCTCATCTTCCTTGTCCCTCTCTATGAAATTGTCTTTGTCCCTGCTGCAAGAAAAGTGACAGGAAGGGACTCGGGGATCTCACCTTTGCAAAGAGTTGGCACTGGCCTCTTTATTGCTACTTTTTCCATGGTTTCAGCTGCATTGATTGAGCAAAAGAGAAGAAACGCAGCCTTGTATTCGAACCAAGCCCTTTCTATCTTTTGGATTGCTCCACAGTTTCTCATCTTTGGCCTTTCGGAGATGTTCACCGCGGTGGGGCTCATAGAGTTCTTCTACAAGCAGTCATTGGAGGGGATGCAGTCCTTTCTGACTGCCATGACATACTGCTCATACTCGTTCGGCTTCTATTTGAGCTCCCTCCTTGTTTCTCTGGTGAACAAGGTGACCTCAACTTCTTCAAATGGTGGGTGGCTCAGTGACAATGATCTCAACAGGGATAGGCTAGACCGTTTCTATTGGCTGCTCGCTGCGCTCAGCCTCGCCAACTTCTTCAATTACCTTTTCTGGTCTAATTGGTATTCTTACAACCCGTCTTTATCACCGAAAGAGTCCTCTTCCTCCTCCTACGGAAAGGACTTGGAAAGCAATAGCTTCAACCCTTCAAAGCCTGTTGTTGAACCTCACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

638

Amino Acids

70.64

Weight (kDa)

8.5

Isoelectric Point (pI)

45.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013921)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33440
fragaria_vesca FvH4_2g00200
malus_domestica MD05G1000900.v1.1
prunus_persica Prupe.8G001900_v2.0.a1 Prupe.8G001900_v2.0.a1
pyrus_communis pycom05g00010
rosa_chinensis RchiOBHm_Chr6g0249481
rosa_laevigata RLG00000015577
rosa_multiflora Rmu_sc0002916.1_g000002
rosa_roxburghii Rroxscaffold_7G00217660
rosa_rugosa Rorug05G0492600 Rorug05G0492700
rosa_samantha Rh6AG002400 Rh6BG007700 Rh6CG001600 Rh6DG001600
rosa_wichuraiana Rw6G000300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 152
AbsI CCTCGAGG 1 cut(s) 774
Acc36I ACCTGC 1 cut(s) 152
AccII CGCG 2 cut(s) 1232, 1535
AciI CCGC 4 cut(s) 513, 1066, 1533, 1535
AclWI GGATC 3 cut(s) 1076, 1235, 1367
AfaI GTAC 3 cut(s) 192, 748, 1153
AfiI CCNNNNNNNGG 4 cut(s) 661, 776, 1033, 1539
AjnI CCWGG 2 cut(s) 495, 1026
AleI CACNNNNGTG 1 cut(s) 1535
AloI GAACNNNNNNTCC 2 cut(s) 1060, 1092
AluBI AGCT 9 cut(s) 309, 343, 589, 710, 1178, 1256, 1421, 1638, 1875
AluI AGCT 9 cut(s) 309, 343, 589, 710, 1178, 1256, 1421, 1638, 1875
Alw21I GWGCWC 2 cut(s) 345, 1640
Alw26I GTCTC 1 cut(s) 706
AlwI GGATC 3 cut(s) 1076, 1235, 1367
AlwNI CAGNNNCTG 2 cut(s) 81, 710
Ama87I CYCGRG 2 cut(s) 774, 1354
AoxI GGCC 6 cut(s) 392, 468, 501, 546, 1387, 1513
Asp700I GAANNNNTTC 1 cut(s) 1238
AspLEI GCGC 2 cut(s) 611, 1756
AspS9I GGNCC 4 cut(s) 315, 469, 546, 664
AsuC2I CCSGG 1 cut(s) 679
AsuHPI GGTGA 6 cut(s) 975, 1356, 1522, 1669, 1678, 1815
AsuII TTCGAA 1 cut(s) 1463
AvaI CYCGRG 2 cut(s) 774, 1354
AvaII GGWCC 2 cut(s) 315, 664
AxyI CCTNAGG 2 cut(s) 450, 505
BaeGI GKGCMC 1 cut(s) 1140
BanII GRGCYC 3 cut(s) 345, 1545, 1640
BbsI GAAGAC 1 cut(s) 250
Bbv12I GWGCWC 2 cut(s) 345, 1640
BccI CCATC 6 cut(s) 145, 384, 676, 1049, 1163, 1277
BciT130I CCWGG 2 cut(s) 497, 1028
BclI TGATCA 1 cut(s) 321
BcnI CCSGG 1 cut(s) 679
BcoDI GTCTC 1 cut(s) 706
BfaI CTAG 2 cut(s) 1224, 1727
BfmI CTRYAG 1 cut(s) 134
BfuAI ACCTGC 1 cut(s) 152
BlpI GCTNAGC 1 cut(s) 1757
Bme1390I CCNGG 3 cut(s) 497, 679, 1028
Bme18I GGWCC 2 cut(s) 315, 664
BmeT110I CYCGRG 2 cut(s) 774, 1354
BmgT120I GGNCC 4 cut(s) 315, 469, 546, 664
BmiI GGNNCC 2 cut(s) 547, 665
BmrFI CCNGG 3 cut(s) 497, 679, 1028
BmsI GCATC 3 cut(s) 192, 1050, 1569
BpiI GAAGAC 1 cut(s) 250
BpmI CTGGAG 1 cut(s) 1169
Bpu1102I GCTNAGC 1 cut(s) 1757
Bpu14I TTCGAA 1 cut(s) 1463
BpuEI CTTGAG 1 cut(s) 786
BpuMI CCSGG 1 cut(s) 679
BsaAI YACGTR 1 cut(s) 186
BsaJI CCNNGG 7 cut(s) 97, 127, 274, 301, 714, 1410, 1533
BsaWI WCCGGW 1 cut(s) 629
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bsc4I CCNNNNNNNGG 4 cut(s) 661, 776, 1033, 1539
Bse1I ACTGG 2 cut(s) 553, 1390
Bse21I CCTNAGG 2 cut(s) 450, 505
Bse3DI GCAATG 2 cut(s) 639, 726
BseBI CCWGG 2 cut(s) 497, 1028
BseDI CCNNGG 7 cut(s) 97, 127, 274, 301, 714, 1410, 1533
BseGI GGATG 5 cut(s) 419, 687, 691, 1269, 1584
BseLI CCNNNNNNNGG 4 cut(s) 661, 776, 1033, 1539
BseMI GCAATG 2 cut(s) 639, 726
BseMII CTCAG 5 cut(s) 441, 717, 1143, 1711, 1771
BseNI ACTGG 2 cut(s) 553, 1390
BseRI GAGGAG 6 cut(s) 27, 254, 449, 452, 992, 1834
BseSI GKGCMC 1 cut(s) 1140
BseYI CCCAGC 1 cut(s) 1252
BsgI GTGCAG 2 cut(s) 54, 610
Bsh1236I CGCG 2 cut(s) 1232, 1535
BshFI GGCC 6 cut(s) 394, 470, 503, 548, 1389, 1515
BsiHKAI GWGCWC 2 cut(s) 345, 1640
BsiHKCI CYCGRG 2 cut(s) 774, 1354
BsiSI CCGG 2 cut(s) 630, 678
BslFI GGGAC 5 cut(s) 265, 578, 1283, 1307, 1364
BslI CCNNNNNNNGG 4 cut(s) 661, 776, 1033, 1539
BsmAI GTCTC 1 cut(s) 706
BsmFI GGGAC 5 cut(s) 265, 578, 1283, 1307, 1364
BsnI GGCC 6 cut(s) 394, 470, 503, 548, 1389, 1515
BsoBI CYCGRG 2 cut(s) 774, 1354
Bsp119I TTCGAA 1 cut(s) 1463
Bsp1286I GDGCHC 4 cut(s) 345, 1140, 1545, 1640
Bsp143I GATC 6 cut(s) 321, 823, 1068, 1227, 1359, 1708
Bsp1720I GCTNAGC 1 cut(s) 1757
Bsp19I CCATGG 3 cut(s) 97, 714, 1410
Bsp68I TCGCGA 1 cut(s) 1232
BspACI CCGC 4 cut(s) 513, 1066, 1533, 1535
BspANI GGCC 6 cut(s) 394, 470, 503, 548, 1389, 1515
BspCNI CTCAG 5 cut(s) 442, 716, 1142, 1710, 1770
BspFNI CGCG 2 cut(s) 1232, 1535
BspLI GGNNCC 2 cut(s) 547, 665
BspMAI CTGCAG 1 cut(s) 138
BspMI ACCTGC 1 cut(s) 152
BspPI GGATC 3 cut(s) 1076, 1235, 1367
BspT104I TTCGAA 1 cut(s) 1463
BsrDI GCAATG 2 cut(s) 639, 726
BsrI ACTGG 2 cut(s) 553, 1390
BssECI CCNNGG 7 cut(s) 97, 127, 274, 301, 714, 1410, 1533
BssMI GATC 6 cut(s) 321, 823, 1068, 1227, 1359, 1708
BssT1I CCWWGG 6 cut(s) 97, 127, 274, 301, 714, 1410
Bst2UI CCWGG 2 cut(s) 497, 1028
Bst4CI ACNGT 7 cut(s) 216, 256, 652, 947, 1096, 1500, 1733
Bst6I CTCTTC 1 cut(s) 1843
BstAPI GCANNNNNTGC 1 cut(s) 81
BstBAI YACGTR 1 cut(s) 186
BstBI TTCGAA 1 cut(s) 1463
BstC8I GCNNGC 5 cut(s) 86, 368, 372, 1039, 1749
BstDEI CTNAG 7 cut(s) 450, 505, 582, 703, 1129, 1697, 1757
BstDSI CCRYGG 4 cut(s) 97, 714, 1410, 1533
BstEII GGTNACC 1 cut(s) 1666
BstENI CCTNNNNNAGG 1 cut(s) 1031
BstF5I GGATG 5 cut(s) 419, 687, 691, 1269, 1584
BstFNI CGCG 2 cut(s) 1232, 1535
BstHHI GCGC 2 cut(s) 611, 1756
BstKTI GATC 6 cut(s) 324, 826, 1071, 1230, 1362, 1711
BstMAI GTCTC 1 cut(s) 706
BstMBI GATC 6 cut(s) 321, 823, 1068, 1227, 1359, 1708
BstMWI GCNNNNNNNGC 5 cut(s) 81, 367, 617, 716, 1753
BstNI CCWGG 2 cut(s) 497, 1028
BstNSI RCATGY 2 cut(s) 1041, 1152
BstPI GGTNACC 1 cut(s) 1666
BstSCI CCNGG 3 cut(s) 495, 677, 1026
BstSFI CTRYAG 1 cut(s) 134
BstSLI GKGCMC 1 cut(s) 1140
BstSNI TACGTA 1 cut(s) 186
BstUI CGCG 2 cut(s) 1232, 1535
BstV2I GAAGAC 1 cut(s) 250
BstX2I RGATCY 1 cut(s) 1359
BstXI CCANNNNNNTGG 1 cut(s) 721
BstYI RGATCY 1 cut(s) 1359
Bsu36I CCTNAGG 2 cut(s) 450, 505
BsuRI GGCC 6 cut(s) 394, 470, 503, 548, 1389, 1515
BtgI CCRYGG 4 cut(s) 97, 714, 1410, 1533
BtsCI GGATG 5 cut(s) 419, 687, 691, 1269, 1584
BtsI GCAGTG 1 cut(s) 42
BtsIMutI CAGTG 6 cut(s) 42, 212, 952, 1008, 1383, 1705
BtuMI TCGCGA 1 cut(s) 1232
BveI ACCTGC 1 cut(s) 152
Cac8I GCNNGC 5 cut(s) 86, 368, 372, 1039, 1749
CaiI CAGNNNCTG 2 cut(s) 81, 710
CfoI GCGC 2 cut(s) 611, 1756
Cfr13I GGNCC 4 cut(s) 315, 469, 546, 664
Cfr42I CCGCGG 1 cut(s) 1536
CsiI ACCWGGT 1 cut(s) 495
Csp6I GTAC 3 cut(s) 191, 747, 1152
CspCI CAANNNNNGTGG 2 cut(s) 82, 117
CviQI GTAC 3 cut(s) 191, 747, 1152
DdeI CTNAG 7 cut(s) 450, 505, 582, 703, 1129, 1697, 1757
DpnI GATC 6 cut(s) 323, 825, 1070, 1229, 1361, 1710
DpnII GATC 6 cut(s) 321, 823, 1068, 1227, 1359, 1708
Eam1104I CTCTTC 1 cut(s) 1843
EarI CTCTTC 1 cut(s) 1843
Ecl136II GAGCTC 2 cut(s) 343, 1638
Eco105I TACGTA 1 cut(s) 186
Eco130I CCWWGG 6 cut(s) 97, 127, 274, 301, 714, 1410
Eco24I GRGCYC 3 cut(s) 345, 1545, 1640
Eco47I GGWCC 2 cut(s) 315, 664
Eco53kI GAGCTC 2 cut(s) 343, 1638
Eco81I CCTNAGG 2 cut(s) 450, 505
Eco88I CYCGRG 2 cut(s) 774, 1354
Eco91I GGTNACC 1 cut(s) 1666
EcoICRI GAGCTC 2 cut(s) 343, 1638
EcoNI CCTNNNNNAGG 1 cut(s) 1031
EcoO109I RGGNCCY 1 cut(s) 469
EcoO65I GGTNACC 1 cut(s) 1666
EcoRII CCWGG 2 cut(s) 495, 1026
EcoT14I CCWWGG 6 cut(s) 97, 127, 274, 301, 714, 1410
EcoT22I ATGCAT 1 cut(s) 1043
EcoT38I GRGCYC 3 cut(s) 345, 1545, 1640
ErhI CCWWGG 6 cut(s) 97, 127, 274, 301, 714, 1410
FaqI GGGAC 5 cut(s) 265, 578, 1283, 1307, 1364
FbaI TGATCA 1 cut(s) 321
FokI GGATG 5 cut(s) 406, 694, 698, 1256, 1591
FriOI GRGCYC 3 cut(s) 345, 1545, 1640
FspBI CTAG 2 cut(s) 1224, 1727
GlaI GCGC 2 cut(s) 610, 1755
GsaI CCCAGC 1 cut(s) 1256
GsuI CTGGAG 1 cut(s) 1169
HaeIII GGCC 6 cut(s) 394, 470, 503, 548, 1389, 1515
HapII CCGG 2 cut(s) 630, 678
HhaI GCGC 2 cut(s) 611, 1756
Hin6I GCGC 2 cut(s) 609, 1754
HinP1I GCGC 2 cut(s) 609, 1754
HinfI GANTC 8 cut(s) 17, 44, 293, 569, 699, 1023, 1352, 1832
HpaII CCGG 2 cut(s) 630, 678
HphI GGTGA 6 cut(s) 975, 1356, 1522, 1669, 1678, 1815
Hpy166II GTNNAC 3 cut(s) 888, 1530, 1660
Hpy188I TCNGA 6 cut(s) 49, 180, 292, 1022, 1522, 1593
Hpy188III TCNNGA 6 cut(s) 238, 313, 449, 559, 1186, 1231
Hpy8I GTNNAC 3 cut(s) 888, 1530, 1660
Hpy99I CGWCG 1 cut(s) 1100
HpyAV CCTTC 5 cut(s) 631, 852, 921, 1341, 1893
HpyCH4III ACNGT 7 cut(s) 216, 256, 652, 947, 1096, 1500, 1733
HpyCH4IV ACGT 2 cut(s) 185, 990
HpyF10VI GCNNNNNNNGC 5 cut(s) 81, 367, 617, 716, 1753
HpyF3I CTNAG 7 cut(s) 450, 505, 582, 703, 1129, 1697, 1757
HpySE526I ACGT 2 cut(s) 185, 990
HspAI GCGC 2 cut(s) 609, 1754
Ksp22I TGATCA 1 cut(s) 321
KspI CCGCGG 1 cut(s) 1536
Kzo9I GATC 6 cut(s) 321, 823, 1068, 1227, 1359, 1708
LmnI GCTCC 4 cut(s) 306, 1108, 1498, 1643
LweI GCATC 3 cut(s) 192, 1050, 1569
MabI ACCWGGT 1 cut(s) 495
MaeI CTAG 2 cut(s) 1224, 1727
MaeII ACGT 2 cut(s) 185, 990
MaeIII GTNAC 6 cut(s) 235, 981, 991, 1339, 1666, 1700
MalI GATC 6 cut(s) 323, 825, 1070, 1229, 1361, 1710
MboI GATC 6 cut(s) 321, 823, 1068, 1227, 1359, 1708
MflI RGATCY 1 cut(s) 1359
MhlI GDGCHC 4 cut(s) 345, 1140, 1545, 1640
MlyI GAGTC 4 cut(s) 563, 708, 1346, 1841
Mph1103I ATGCAT 1 cut(s) 1043
MroXI GAANNNNTTC 1 cut(s) 1238
MseI TTAA 4 cut(s) 8, 389, 810, 1912
MslI CAYNNNNRTG 3 cut(s) 429, 719, 1535
MspA1I CMGCKG 4 cut(s) 710, 1066, 1421, 1535
MspI CCGG 2 cut(s) 630, 678
MspR9I CCNGG 3 cut(s) 497, 679, 1028
MvaI CCWGG 2 cut(s) 497, 1028
MvnI CGCG 2 cut(s) 1232, 1535
MwoI GCNNNNNNNGC 5 cut(s) 81, 367, 617, 716, 1753
NciI CCSGG 1 cut(s) 679
NcoI CCATGG 3 cut(s) 97, 714, 1410
NdeII GATC 6 cut(s) 321, 823, 1068, 1227, 1359, 1708
NlaIV GGNNCC 2 cut(s) 547, 665
NmuCI GTSAC 6 cut(s) 235, 981, 991, 1339, 1666, 1700
NruI TCGCGA 1 cut(s) 1232
NsiI ATGCAT 1 cut(s) 1043
NspI RCATGY 2 cut(s) 1041, 1152
NspV TTCGAA 1 cut(s) 1463
OliI CACNNNNGTG 1 cut(s) 1535
PaeI GCATGC 1 cut(s) 1041
PaeR7I CTCGAG 1 cut(s) 774
PaqCI CACCTGC 1 cut(s) 152
PcsI WCGNNNNNNNCGW 1 cut(s) 336
PdmI GAANNNNTTC 1 cut(s) 1238
PfeI GAWTC 4 cut(s) 17, 44, 293, 1023
PfoI TCCNGGA 2 cut(s) 677, 1026
PleI GAGTC 4 cut(s) 563, 707, 1346, 1840
PpsI GAGTC 4 cut(s) 563, 707, 1346, 1840
Ppu21I YACGTR 1 cut(s) 186
Psp124BI GAGCTC 2 cut(s) 345, 1640
Psp6I CCWGG 2 cut(s) 495, 1026
PspEI GGTNACC 1 cut(s) 1666
PspFI CCCAGC 1 cut(s) 1252
PspGI CCWGG 2 cut(s) 495, 1026
PspN4I GGNNCC 2 cut(s) 547, 665
PspPI GGNCC 4 cut(s) 315, 469, 546, 664
PspXI VCTCGAGB 1 cut(s) 774
PstI CTGCAG 1 cut(s) 138
PstNI CAGNNNCTG 2 cut(s) 81, 710
PsuI RGATCY 1 cut(s) 1359
PvuII CAGCTG 2 cut(s) 710, 1421
RruI TCGCGA 1 cut(s) 1232
RsaI GTAC 3 cut(s) 192, 748, 1153
RsaNI GTAC 3 cut(s) 191, 747, 1152
RseI CAYNNNNRTG 3 cut(s) 429, 719, 1535
SacI GAGCTC 2 cut(s) 345, 1640
SacII CCGCGG 1 cut(s) 1536
SaqAI TTAA 4 cut(s) 8, 389, 810, 1912
Sau3AI GATC 6 cut(s) 321, 823, 1068, 1227, 1359, 1708
Sau96I GGNCC 4 cut(s) 315, 469, 546, 664
SchI GAGTC 4 cut(s) 563, 708, 1346, 1841
ScrFI CCNGG 3 cut(s) 497, 679, 1028
SduI GDGCHC 4 cut(s) 345, 1140, 1545, 1640
SexAI ACCWGGT 1 cut(s) 495
SfaNI GCATC 3 cut(s) 192, 1050, 1569
SfcI CTRYAG 1 cut(s) 134
Sfr274I CTCGAG 1 cut(s) 774
Sfr303I CCGCGG 1 cut(s) 1536
SfuI TTCGAA 1 cut(s) 1463
SgrBI CCGCGG 1 cut(s) 1536
SinI GGWCC 2 cut(s) 315, 664
SlaI CTCGAG 1 cut(s) 774
SmiMI CAYNNNNRTG 3 cut(s) 429, 719, 1535
SmlI CTYRAG 2 cut(s) 774, 801
SmoI CTYRAG 2 cut(s) 774, 801
SnaBI TACGTA 1 cut(s) 186
SphI GCATGC 1 cut(s) 1041
SsiI CCGC 4 cut(s) 513, 1066, 1533, 1535
SspI AATATT 1 cut(s) 412
SspMI CTAG 2 cut(s) 1224, 1727
SstI GAGCTC 2 cut(s) 345, 1640
StyD4I CCNGG 3 cut(s) 495, 677, 1026
StyI CCWWGG 6 cut(s) 97, 127, 274, 301, 714, 1410
TaaI ACNGT 7 cut(s) 216, 256, 652, 947, 1096, 1500, 1733
TaiI ACGT 2 cut(s) 188, 993
TaqI TCGA 4 cut(s) 339, 775, 1071, 1463
TatI WGTACW 1 cut(s) 190
TfiI GAWTC 4 cut(s) 17, 44, 293, 1023
Tru1I TTAA 4 cut(s) 8, 389, 810, 1912
Tru9I TTAA 4 cut(s) 8, 389, 810, 1912
TscAI CASTG 6 cut(s) 42, 219, 952, 1015, 1390, 1705
TseFI GTSAC 6 cut(s) 235, 981, 991, 1339, 1666, 1700
Tsp45I GTSAC 6 cut(s) 235, 981, 991, 1339, 1666, 1700
TspDTI ATGAA 5 cut(s) 17, 235, 285, 1232, 1323
TspGWI ACGGA 2 cut(s) 1186, 1866
TspRI CASTG 6 cut(s) 42, 219, 952, 1015, 1390, 1705
VpaK11BI GGWCC 2 cut(s) 315, 664
XagI CCTNNNNNAGG 1 cut(s) 1031
XceI RCATGY 2 cut(s) 1041, 1152
XhoI CTCGAG 1 cut(s) 774
XmnI GAANNNNTTC 1 cut(s) 1238
XspI CTAG 2 cut(s) 1224, 1727
Zsp2I ATGCAT 1 cut(s) 1043
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.