MD05G1000900.v1.1

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0013921
Physical Location & Seq
Forward (+)
293868 .. 298577
4710 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1000900.v1.1.491

Sequence Viewer

Length: 1845 bp
ATGAAATTAAGAGGAGATTCAAAAAGTGAGGTTGCAGTGGTGGATTCGGAGGATATGTCTGTTGATTGGAGAGGCAGACCCTGTAAGCCTACCAACCATGGTGGAATGAGGGCTGCTGTTTTTGTCCTTGGTCTGCAGGGTTTTGAGATGATGGCAATAGCAGGTGTTGGGAACAACCTGATTACGTATGTACTAAGTGAGATGCACTTTTCACTGTCAAAGTCTGCAAACATAGTCACGAACTTCATAGGGACAGTCTTCCTCCTCTCCCTCCTTGGTGGCTACCTCTCTGATTCATACCTTGGGAGCTTCTGGACCATGATCATCTTCGGCTTTGTCGAGCTCTCTGGTTTTATATTGCTGTGCGTGCAAGCCCATCTCCCACAATTAAGGCCACTCTCCTGCAACAATATTATGTCATCCCATGAGCATGACGAGGAGTTATGTCCTGAGGCAAAGGGCTACAAGGCCTTGATATTCTTTGCTGCAATATACCTGGTGGCCTTAGGAAGCGGTTGCCTAAAACCAAACATAATCTCTCATGGGGCCGACCAGTTCAGGAGGGTCGACTCCAAGCAGTCTAAGAAGCTATCGTCTTACTTCAACTGCGCTTACTTCGCCTTCTGCACCGGAGAACTCATTGCACTAACAGTCCTTGTTTGGGTCCAAACAAACTCCGGGATGGATGTTGGGTTTGGAGTCTCAGCAGCTGCCATGGCAATGGGATTATTCTGCTTGACTTTTGGTACACCTCTTTACAGGAACAAACCCCCTCGAGGAAGTATTTTCACTCCTATTGCTCAAGTTTTTGTTGCTGCGTTTACAAAGAGAAAGCAAATCTGTCCTTCAAATACAGAAATGCTTCATGGAAACCGAAACAGTGTGCCAACGTCAAACCACCTCTTTGTTCCCATGTCACCAAACGTCACTACTCTCCTCCACACTGAAAAGTTCAGATTCCTGGACAAGGCATGCATCAAAATTGAAGATGGAAGTCAGCGGATTGAAAGTTCTTGGAGATTATGCACCGTCGCACAGGTGGAGCAAGTCAAAATCATTTTCTCAATTGTGCCCATTTTTGCATGTACCATCATCTTCAACACCATTTTAGCTCAACTCCAGACATTCTCCGTCCAGCAAGGAAGTTCCATGAACACTAAGATCACGAAAGGTTTCAAAGTTCCCCCAGCTTCGCTTCAAGCCATCCCTTACATTATGCTCATCTTCCTTGTCCCTCTCTATGAAATTGTCTTTGTCCCTGCTGCAAGAAAAGTGACAGGAAGGGACTCGGGGATCTCACCTTTGCAAAGAGTTGGCGCTGGCCTCTTTATTGCTACTTTTTCCATGGTTTCAGCTGCATTGATTGAACAAAAGAGAAGAAACGCAGCCTTATATTCGAACCAAGCCCTTTCTATCTTTTGGATTGCTCCACAGTTTCTCATCTTTGGCCTTTCAGAGATGTTCACCGCGGTGGGGCTCATAGAGTTCTTCTACAAGCAGTCACTGGAGGGGATGCAGTCCTTTCTGACTGCCATGACATACTGCTCATACTCGTTCGGCTTCTATTTGAGCTCCCTCCTTGTTTCTCTGGTGAACAAGGTGACCTCAACTTCTTCAAATGGTGGGTGGCTCAGTGACAATGATCTCAACAGGGATAGGCTAGACCGTTTCTATTGGCTGCTCGCTGCGCTCAGCCTCGTCAACTTCTTCAATTACCTTTTCTGGTCTAATTGGTATTCTTACAACCCGTCTTTATCACCGAAAGAGTCCTCTTCCTCCTACGGAAAGGACTTGGAAAGCAATGGCTTCAACCCTTCAAAGCCTGTTGTTGAACCTCACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

615

Amino Acids

68.09

Weight (kDa)

8.81

Isoelectric Point (pI)

43.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 104 - 543 2.8e-87 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013921)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33440
fragaria_vesca FvH4_2g00200
malus_domestica MD05G1000900.v1.1
prunus_persica Prupe.8G001900_v2.0.a1 Prupe.8G001900_v2.0.a1
pyrus_communis pycom05g00010
rosa_chinensis RchiOBHm_Chr6g0249481
rosa_laevigata RLG00000015577
rosa_multiflora Rmu_sc0002916.1_g000002
rosa_roxburghii Rroxscaffold_7G00217660
rosa_rugosa Rorug05G0492600 Rorug05G0492700
rosa_samantha Rh6AG002400 Rh6BG007700 Rh6CG001600 Rh6DG001600
rosa_wichuraiana Rw6G000300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 152
AbsI CCTCGAGG 1 cut(s) 774
Acc36I ACCTGC 1 cut(s) 152
AccI GTMKAC 1 cut(s) 567
AccII CGCG 1 cut(s) 1469
AciI CCGC 4 cut(s) 513, 1000, 1467, 1469
AclWI GGATC 1 cut(s) 1301
AfaI GTAC 3 cut(s) 192, 748, 1087
AfiI CCNNNNNNNGG 4 cut(s) 661, 776, 967, 1473
AjnI CCWGG 2 cut(s) 495, 960
AleI CACNNNNGTG 1 cut(s) 1469
AloI GAACNNNNNNTCC 2 cut(s) 994, 1026
AluBI AGCT 8 cut(s) 309, 343, 589, 710, 1112, 1190, 1355, 1572
AluI AGCT 8 cut(s) 309, 343, 589, 710, 1112, 1190, 1355, 1572
Alw21I GWGCWC 2 cut(s) 345, 1574
Alw26I GTCTC 1 cut(s) 706
AlwI GGATC 1 cut(s) 1301
AlwNI CAGNNNCTG 3 cut(s) 81, 710, 1504
Ama87I CYCGRG 2 cut(s) 774, 1288
AoxI GGCC 6 cut(s) 392, 468, 501, 546, 1321, 1447
Asp700I GAANNNNTTC 2 cut(s) 861, 1172
AspLEI GCGC 3 cut(s) 611, 1319, 1690
AspS9I GGNCC 3 cut(s) 315, 546, 664
AsuC2I CCSGG 1 cut(s) 679
AsuHPI GGTGA 6 cut(s) 909, 1290, 1456, 1603, 1612, 1749
AsuII TTCGAA 1 cut(s) 1397
AvaI CYCGRG 2 cut(s) 774, 1288
AvaII GGWCC 2 cut(s) 315, 664
AxyI CCTNAGG 2 cut(s) 450, 505
BaeGI GKGCMC 1 cut(s) 1074
BanII GRGCYC 3 cut(s) 345, 1479, 1574
BbsI GAAGAC 1 cut(s) 250
Bbv12I GWGCWC 2 cut(s) 345, 1574
BccI CCATC 6 cut(s) 145, 384, 676, 983, 1097, 1211
BciT130I CCWGG 2 cut(s) 497, 962
BclI TGATCA 1 cut(s) 321
BcnI CCSGG 1 cut(s) 679
BcoDI GTCTC 1 cut(s) 706
BfaI CTAG 1 cut(s) 1661
BfmI CTRYAG 1 cut(s) 134
BfoI RGCGCY 1 cut(s) 1320
BfuAI ACCTGC 1 cut(s) 152
BlpI GCTNAGC 1 cut(s) 1691
Bme1390I CCNGG 3 cut(s) 497, 679, 962
Bme18I GGWCC 2 cut(s) 315, 664
BmeT110I CYCGRG 2 cut(s) 774, 1288
BmgT120I GGNCC 3 cut(s) 315, 546, 664
BmiI GGNNCC 2 cut(s) 547, 665
BmrFI CCNGG 3 cut(s) 497, 679, 962
BmsI GCATC 3 cut(s) 192, 984, 1503
BpiI GAAGAC 1 cut(s) 250
BpmI CTGGAG 2 cut(s) 1103, 1526
Bpu1102I GCTNAGC 1 cut(s) 1691
Bpu14I TTCGAA 1 cut(s) 1397
BpuEI CTTGAG 1 cut(s) 786
BpuMI CCSGG 1 cut(s) 679
BsaAI YACGTR 1 cut(s) 186
BsaJI CCNNGG 7 cut(s) 97, 127, 274, 301, 714, 1344, 1467
BsaWI WCCGGW 1 cut(s) 629
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bsc4I CCNNNNNNNGG 4 cut(s) 661, 776, 967, 1473
Bse1I ACTGG 2 cut(s) 553, 1509
Bse21I CCTNAGG 2 cut(s) 450, 505
Bse3DI GCAATG 3 cut(s) 639, 726, 1807
BseBI CCWGG 2 cut(s) 497, 962
BseDI CCNNGG 7 cut(s) 97, 127, 274, 301, 714, 1344, 1467
BseGI GGATG 5 cut(s) 419, 687, 691, 1203, 1518
BseLI CCNNNNNNNGG 4 cut(s) 661, 776, 967, 1473
BseMI GCAATG 3 cut(s) 639, 726, 1807
BseMII CTCAG 4 cut(s) 441, 717, 1645, 1705
BseNI ACTGG 2 cut(s) 553, 1509
BseRI GAGGAG 4 cut(s) 27, 254, 452, 926
BseSI GKGCMC 1 cut(s) 1074
BseYI CCCAGC 1 cut(s) 1186
BsgI GTGCAG 1 cut(s) 610
Bsh1236I CGCG 1 cut(s) 1469
BshFI GGCC 6 cut(s) 394, 470, 503, 548, 1323, 1449
BsiHKAI GWGCWC 2 cut(s) 345, 1574
BsiHKCI CYCGRG 2 cut(s) 774, 1288
BsiSI CCGG 2 cut(s) 630, 678
BslFI GGGAC 4 cut(s) 265, 1217, 1241, 1298
BslI CCNNNNNNNGG 4 cut(s) 661, 776, 967, 1473
BsmAI GTCTC 1 cut(s) 706
BsmFI GGGAC 4 cut(s) 265, 1217, 1241, 1298
BsnI GGCC 6 cut(s) 394, 470, 503, 548, 1323, 1449
BsoBI CYCGRG 2 cut(s) 774, 1288
Bsp119I TTCGAA 1 cut(s) 1397
Bsp1286I GDGCHC 4 cut(s) 345, 1074, 1479, 1574
Bsp143I GATC 4 cut(s) 321, 1161, 1293, 1642
Bsp1720I GCTNAGC 1 cut(s) 1691
Bsp19I CCATGG 3 cut(s) 97, 714, 1344
BspACI CCGC 4 cut(s) 513, 1000, 1467, 1469
BspANI GGCC 6 cut(s) 394, 470, 503, 548, 1323, 1449
BspCNI CTCAG 4 cut(s) 442, 716, 1644, 1704
BspFNI CGCG 1 cut(s) 1469
BspLI GGNNCC 2 cut(s) 547, 665
BspMAI CTGCAG 1 cut(s) 138
BspMI ACCTGC 1 cut(s) 152
BspPI GGATC 1 cut(s) 1301
BspT104I TTCGAA 1 cut(s) 1397
BsrDI GCAATG 3 cut(s) 639, 726, 1807
BsrI ACTGG 2 cut(s) 553, 1509
BssECI CCNNGG 7 cut(s) 97, 127, 274, 301, 714, 1344, 1467
BssMI GATC 4 cut(s) 321, 1161, 1293, 1642
BssT1I CCWWGG 6 cut(s) 97, 127, 274, 301, 714, 1344
Bst2UI CCWGG 2 cut(s) 497, 962
Bst4CI ACNGT 7 cut(s) 216, 256, 652, 881, 1030, 1434, 1667
Bst6I CTCTTC 1 cut(s) 1777
BstBAI YACGTR 1 cut(s) 186
BstBI TTCGAA 1 cut(s) 1397
BstC8I GCNNGC 5 cut(s) 368, 372, 973, 1321, 1683
BstDEI CTNAG 8 cut(s) 194, 450, 505, 582, 703, 1158, 1631, 1691
BstDSI CCRYGG 4 cut(s) 97, 714, 1344, 1467
BstEII GGTNACC 1 cut(s) 1600
BstENI CCTNNNNNAGG 1 cut(s) 965
BstF5I GGATG 5 cut(s) 419, 687, 691, 1203, 1518
BstFNI CGCG 1 cut(s) 1469
BstH2I RGCGCY 1 cut(s) 1320
BstHHI GCGC 3 cut(s) 611, 1319, 1690
BstKTI GATC 4 cut(s) 324, 1164, 1296, 1645
BstMAI GTCTC 1 cut(s) 706
BstMBI GATC 4 cut(s) 321, 1161, 1293, 1642
BstMWI GCNNNNNNNGC 4 cut(s) 367, 617, 716, 1687
BstNI CCWGG 2 cut(s) 497, 962
BstNSI RCATGY 2 cut(s) 975, 1086
BstPI GGTNACC 1 cut(s) 1600
BstSCI CCNGG 3 cut(s) 495, 677, 960
BstSFI CTRYAG 1 cut(s) 134
BstSLI GKGCMC 1 cut(s) 1074
BstSNI TACGTA 1 cut(s) 186
BstUI CGCG 1 cut(s) 1469
BstV2I GAAGAC 1 cut(s) 250
BstX2I RGATCY 1 cut(s) 1293
BstXI CCANNNNNNTGG 1 cut(s) 721
BstYI RGATCY 1 cut(s) 1293
Bsu36I CCTNAGG 2 cut(s) 450, 505
BsuRI GGCC 6 cut(s) 394, 470, 503, 548, 1323, 1449
BtgI CCRYGG 4 cut(s) 97, 714, 1344, 1467
BtsCI GGATG 5 cut(s) 419, 687, 691, 1203, 1518
BtsI GCAGTG 1 cut(s) 42
BtsIMutI CAGTG 6 cut(s) 42, 212, 886, 942, 1502, 1639
BveI ACCTGC 1 cut(s) 152
Cac8I GCNNGC 5 cut(s) 368, 372, 973, 1321, 1683
CaiI CAGNNNCTG 3 cut(s) 81, 710, 1504
CfoI GCGC 3 cut(s) 611, 1319, 1690
Cfr13I GGNCC 3 cut(s) 315, 546, 664
Cfr42I CCGCGG 1 cut(s) 1470
CsiI ACCWGGT 1 cut(s) 495
Csp6I GTAC 3 cut(s) 191, 747, 1086
CspCI CAANNNNNGTGG 2 cut(s) 82, 117
CviQI GTAC 3 cut(s) 191, 747, 1086
DdeI CTNAG 8 cut(s) 194, 450, 505, 582, 703, 1158, 1631, 1691
DpnI GATC 4 cut(s) 323, 1163, 1295, 1644
DpnII GATC 4 cut(s) 321, 1161, 1293, 1642
Eam1104I CTCTTC 1 cut(s) 1777
EarI CTCTTC 1 cut(s) 1777
Ecl136II GAGCTC 2 cut(s) 343, 1572
Eco105I TACGTA 1 cut(s) 186
Eco130I CCWWGG 6 cut(s) 97, 127, 274, 301, 714, 1344
Eco147I AGGCCT 1 cut(s) 470
Eco24I GRGCYC 3 cut(s) 345, 1479, 1574
Eco47I GGWCC 2 cut(s) 315, 664
Eco53kI GAGCTC 2 cut(s) 343, 1572
Eco81I CCTNAGG 2 cut(s) 450, 505
Eco88I CYCGRG 2 cut(s) 774, 1288
Eco91I GGTNACC 1 cut(s) 1600
EcoICRI GAGCTC 2 cut(s) 343, 1572
EcoNI CCTNNNNNAGG 1 cut(s) 965
EcoO65I GGTNACC 1 cut(s) 1600
EcoRII CCWGG 2 cut(s) 495, 960
EcoT14I CCWWGG 6 cut(s) 97, 127, 274, 301, 714, 1344
EcoT22I ATGCAT 1 cut(s) 977
EcoT38I GRGCYC 3 cut(s) 345, 1479, 1574
ErhI CCWWGG 6 cut(s) 97, 127, 274, 301, 714, 1344
FaqI GGGAC 4 cut(s) 265, 1217, 1241, 1298
FbaI TGATCA 1 cut(s) 321
FblI GTMKAC 1 cut(s) 567
FokI GGATG 5 cut(s) 406, 694, 698, 1190, 1525
FriOI GRGCYC 3 cut(s) 345, 1479, 1574
FspBI CTAG 1 cut(s) 1661
GlaI GCGC 3 cut(s) 610, 1318, 1689
GsaI CCCAGC 1 cut(s) 1190
GsuI CTGGAG 2 cut(s) 1103, 1526
HaeII RGCGCY 1 cut(s) 1320
HaeIII GGCC 6 cut(s) 394, 470, 503, 548, 1323, 1449
HapII CCGG 2 cut(s) 630, 678
HhaI GCGC 3 cut(s) 611, 1319, 1690
Hin6I GCGC 3 cut(s) 609, 1317, 1688
HinP1I GCGC 3 cut(s) 609, 1317, 1688
HincII GTYRAC 2 cut(s) 568, 1702
HindII GTYRAC 2 cut(s) 568, 1702
HinfI GANTC 8 cut(s) 17, 44, 293, 569, 699, 957, 1286, 1766
HpaII CCGG 2 cut(s) 630, 678
HphI GGTGA 6 cut(s) 909, 1290, 1456, 1603, 1612, 1749
Hpy166II GTNNAC 6 cut(s) 568, 749, 822, 1464, 1594, 1702
Hpy188I TCNGA 5 cut(s) 49, 292, 956, 1456, 1527
Hpy188III TCNNGA 6 cut(s) 238, 313, 449, 559, 1120, 1165
Hpy8I GTNNAC 6 cut(s) 568, 749, 822, 1464, 1594, 1702
Hpy99I CGWCG 1 cut(s) 1034
HpyAV CCTTC 4 cut(s) 631, 855, 1275, 1824
HpyCH4III ACNGT 7 cut(s) 216, 256, 652, 881, 1030, 1434, 1667
HpyCH4IV ACGT 3 cut(s) 185, 890, 924
HpyF10VI GCNNNNNNNGC 4 cut(s) 367, 617, 716, 1687
HpyF3I CTNAG 8 cut(s) 194, 450, 505, 582, 703, 1158, 1631, 1691
HpySE526I ACGT 3 cut(s) 185, 890, 924
HspAI GCGC 3 cut(s) 609, 1317, 1688
Ksp22I TGATCA 1 cut(s) 321
KspI CCGCGG 1 cut(s) 1470
Kzo9I GATC 4 cut(s) 321, 1161, 1293, 1642
LmnI GCTCC 4 cut(s) 306, 1042, 1432, 1577
LweI GCATC 3 cut(s) 192, 984, 1503
MabI ACCWGGT 1 cut(s) 495
MaeI CTAG 1 cut(s) 1661
MaeII ACGT 3 cut(s) 185, 890, 924
MaeIII GTNAC 7 cut(s) 235, 915, 925, 1273, 1500, 1600, 1634
MalI GATC 4 cut(s) 323, 1163, 1295, 1644
MboI GATC 4 cut(s) 321, 1161, 1293, 1642
MfeI CAATTG 1 cut(s) 1065
MflI RGATCY 1 cut(s) 1293
MhlI GDGCHC 4 cut(s) 345, 1074, 1479, 1574
MluCI AATT 8 cut(s) 5, 386, 981, 1065, 1245, 1711, 1729, 1840
MlyI GAGTC 4 cut(s) 563, 708, 1280, 1775
Mph1103I ATGCAT 1 cut(s) 977
MroXI GAANNNNTTC 2 cut(s) 861, 1172
MseI TTAA 3 cut(s) 8, 389, 1843
MslI CAYNNNNRTG 3 cut(s) 429, 719, 1469
MspA1I CMGCKG 4 cut(s) 710, 1000, 1355, 1469
MspI CCGG 2 cut(s) 630, 678
MspR9I CCNGG 3 cut(s) 497, 679, 962
MunI CAATTG 1 cut(s) 1065
MvaI CCWGG 2 cut(s) 497, 962
MvnI CGCG 1 cut(s) 1469
MwoI GCNNNNNNNGC 4 cut(s) 367, 617, 716, 1687
NciI CCSGG 1 cut(s) 679
NcoI CCATGG 3 cut(s) 97, 714, 1344
NdeII GATC 4 cut(s) 321, 1161, 1293, 1642
NlaIV GGNNCC 2 cut(s) 547, 665
NmuCI GTSAC 7 cut(s) 235, 915, 925, 1273, 1500, 1600, 1634
NsiI ATGCAT 1 cut(s) 977
NspI RCATGY 2 cut(s) 975, 1086
NspV TTCGAA 1 cut(s) 1397
OliI CACNNNNGTG 1 cut(s) 1469
PaeI GCATGC 1 cut(s) 975
PaeR7I CTCGAG 1 cut(s) 774
PaqCI CACCTGC 1 cut(s) 152
PceI AGGCCT 1 cut(s) 470
PcsI WCGNNNNNNNCGW 1 cut(s) 336
PdmI GAANNNNTTC 2 cut(s) 861, 1172
PfeI GAWTC 4 cut(s) 17, 44, 293, 957
PfoI TCCNGGA 2 cut(s) 677, 960
PleI GAGTC 4 cut(s) 563, 707, 1280, 1774
PpsI GAGTC 4 cut(s) 563, 707, 1280, 1774
Ppu21I YACGTR 1 cut(s) 186
Psp124BI GAGCTC 2 cut(s) 345, 1574
Psp6I CCWGG 2 cut(s) 495, 960
PspEI GGTNACC 1 cut(s) 1600
PspFI CCCAGC 1 cut(s) 1186
PspGI CCWGG 2 cut(s) 495, 960
PspN4I GGNNCC 2 cut(s) 547, 665
PspPI GGNCC 3 cut(s) 315, 546, 664
PspXI VCTCGAGB 1 cut(s) 774
PstI CTGCAG 1 cut(s) 138
PstNI CAGNNNCTG 3 cut(s) 81, 710, 1504
PsuI RGATCY 1 cut(s) 1293
PvuII CAGCTG 2 cut(s) 710, 1355
RsaI GTAC 3 cut(s) 192, 748, 1087
RsaNI GTAC 3 cut(s) 191, 747, 1086
RseI CAYNNNNRTG 3 cut(s) 429, 719, 1469
SacI GAGCTC 2 cut(s) 345, 1574
SacII CCGCGG 1 cut(s) 1470
SalI GTCGAC 1 cut(s) 566
SaqAI TTAA 3 cut(s) 8, 389, 1843
Sau3AI GATC 4 cut(s) 321, 1161, 1293, 1642
Sau96I GGNCC 3 cut(s) 315, 546, 664
SchI GAGTC 4 cut(s) 563, 708, 1280, 1775
ScrFI CCNGG 3 cut(s) 497, 679, 962
SduI GDGCHC 4 cut(s) 345, 1074, 1479, 1574
SexAI ACCWGGT 1 cut(s) 495
SfaNI GCATC 3 cut(s) 192, 984, 1503
SfcI CTRYAG 1 cut(s) 134
Sfr274I CTCGAG 1 cut(s) 774
Sfr303I CCGCGG 1 cut(s) 1470
SfuI TTCGAA 1 cut(s) 1397
SgrBI CCGCGG 1 cut(s) 1470
SinI GGWCC 2 cut(s) 315, 664
SlaI CTCGAG 1 cut(s) 774
SmiMI CAYNNNNRTG 3 cut(s) 429, 719, 1469
SmlI CTYRAG 2 cut(s) 774, 801
SmoI CTYRAG 2 cut(s) 774, 801
SnaBI TACGTA 1 cut(s) 186
SphI GCATGC 1 cut(s) 975
Sse9I AATT 8 cut(s) 5, 386, 981, 1065, 1245, 1711, 1729, 1840
SseBI AGGCCT 1 cut(s) 470
SsiI CCGC 4 cut(s) 513, 1000, 1467, 1469
SspI AATATT 1 cut(s) 412
SspMI CTAG 1 cut(s) 1661
SstI GAGCTC 2 cut(s) 345, 1574
StuI AGGCCT 1 cut(s) 470
StyD4I CCNGG 3 cut(s) 495, 677, 960
StyI CCWWGG 6 cut(s) 97, 127, 274, 301, 714, 1344
TaaI ACNGT 7 cut(s) 216, 256, 652, 881, 1030, 1434, 1667
TaiI ACGT 3 cut(s) 188, 893, 927
TaqI TCGA 4 cut(s) 339, 567, 775, 1397
TasI AATT 8 cut(s) 5, 386, 981, 1065, 1245, 1711, 1729, 1840
TatI WGTACW 1 cut(s) 190
TfiI GAWTC 4 cut(s) 17, 44, 293, 957
Tru1I TTAA 3 cut(s) 8, 389, 1843
Tru9I TTAA 3 cut(s) 8, 389, 1843
TscAI CASTG 6 cut(s) 42, 219, 886, 949, 1509, 1639
TseFI GTSAC 7 cut(s) 235, 915, 925, 1273, 1500, 1600, 1634
Tsp45I GTSAC 7 cut(s) 235, 915, 925, 1273, 1500, 1600, 1634
TspDTI ATGAA 6 cut(s) 17, 235, 285, 854, 1166, 1257
TspGWI ACGGA 2 cut(s) 1120, 1797
TspRI CASTG 6 cut(s) 42, 219, 886, 949, 1509, 1639
VpaK11BI GGWCC 2 cut(s) 315, 664
XagI CCTNNNNNAGG 1 cut(s) 965
XceI RCATGY 2 cut(s) 975, 1086
XhoI CTCGAG 1 cut(s) 774
XmiI GTMKAC 1 cut(s) 567
XmnI GAANNNNTTC 2 cut(s) 861, 1172
XspI CTAG 1 cut(s) 1661
Zsp2I ATGCAT 1 cut(s) 977
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.