RLG00000015577

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0013921
Physical Location & Seq
Reverse (-)
68017410 .. 68021336
3927 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015577

Sequence Viewer

Length: 1848 bp
ATGAAACTGAGAGGAGATTCCATGTCCATCTCTGAGGCTGCAGATGCGGTGGAGGATATCTCTGCTGATTGGAGAGGCAGACCCTGTAAGCCAACCAAACATGGTGGAATGAAGGCTGCTGTTTTTGTCCTAGGTCTACAAGGTTTTGAGATGATGGCAATAGCAGCCGTTGGGAACAATCTCATCACCTATGTACTAAATGAGATGCACTTTCCACTGTCAAAGTCTGCAAACATAGTGACCAACTTTATAGGGACAGTCTTCCTCCTCTCCCTCTTTGGAGGGTTCCTATCAGATTCTTATCTTGGGAGCTTCTGGACCATGTTAATCTTTGGGTTTGTGGAGCTTTCTGGTTTTATACTACTCTCGGTTCAAGCCCATCTCCCCCAGCTAAGGCCACCCTCCTGCAAGGTTATGTCGTCCGAGTACTGTCAGGAGGCAAAGGGCTACAAGGCTCTGATATTCTTTGCTGCAATTTACTTGGTGGCCTTAGGAAGTGGTTGTCTAAAACCAAACATAATCTCTCATGGAGCTGACCAGTTCAGGAAGGAGGATACCAAGCAATCCAAGAAGTTATCAACTTACTTCAATTGTGCATACTTTGCCTTCTGCATGGGAGAACTCATTGCATTAACTGTCCTTGTTTGGGTCCAAACACACTCCGGCATGGATGTTGGATTTGGAGTCTCTGCAGCTGCCATGACACTAGGACTAATTAGCTTGGTGTTTGGTACGTCTCTGTATCGGAACAAACCACCTCGCGGAAGCATTTTCACTCCAATTGCTCAAGTTTTCGTTGCTGCATTTACAAAGAGAAAGCAAATATGCCCCTCAAATATAGAAATGCTTCATGGAAACCAAAACAATGTGCCAAACCACCTCTTTGTCACCATGTCGCCTAACGTCACCACTCTCCTCCACACCGAAAAGTTCAGATTCCTAGACCAGGCATGCATCAAAACAGAAGATGGAACTGAGAGGACCGAAAGTCCATGGAGATTATGCACCGTGTCTCAGGTGGAGCAAGTCAAAATAATTATCTCAGTTGTGCCCATTTTTGCCTGTACCATCATCTTCAACACAATTTTGGCTCAACTCCAAACATTTTCAGTCCAACAAGGAAGTACAATGAACGCAAGGATCACCGAAAGCTTCAAAATCCCCCCAGCTTCACTCCAAGCCATCCCTTACATCATGCTAATCTTTCTTGTCCCTCTCTACGAAACCGTCTTTGTACCGACTGCTCGAAAAGTCACTGGAAGGGACTCTGGGATTTCTCCTTTACAAAGAGTTGGCACTGGCCTTTTTATTGCCACCTTTTCAATGGTTTCAGCTGCAGTTATTGAGAGAAAGAGAAGAAACTCGGCCTTGCATTCGAATGAAGCTCTTTCAATCTTTTGGATTGCTCCACAGTTTCTCATCTTCGGCCTGTCAGAGATGTTCACTGCAGTAGGGCTAATAGAGTTCTTCTATAAGCAGTCATTGGAAGGAATGCAATCATTTCTAACTGCCATGACATACTGCTCATACTCATTTGGCTTCTATCTGAGCTCACTCCTTGTTTCTCTTGTGAACAAAGTTACCTCAACTTCTTCAAGGGGAGGGTGGCTTAGTGACAATGATCTCAACAAGGATAGGCTGGACCGTTTCTATTGGCTGCTGGCTGCTCTCAGTCTTATCAACTTCTTCAATTATCTCTTCTGGTCAAATTGGTATTGTTACAACCCCACTCTATCACCCACTAATACGAATTCACCCCCAGAAGATGGAAAAGACCTGGAAAACAGCAGCAACTTCAAGTACCCTTTAAGGCATATTGAAGGTTCTCATAATATTATTATAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

616

Amino Acids

68.45

Weight (kDa)

8.68

Isoelectric Point (pI)

43.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 105 - 536 5.9e-92 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013921)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33440
fragaria_vesca FvH4_2g00200
malus_domestica MD05G1000900.v1.1
prunus_persica Prupe.8G001900_v2.0.a1 Prupe.8G001900_v2.0.a1
pyrus_communis pycom05g00010
rosa_chinensis RchiOBHm_Chr6g0249481
rosa_laevigata RLG00000015577
rosa_multiflora Rmu_sc0002916.1_g000002
rosa_roxburghii Rroxscaffold_7G00217660
rosa_rugosa Rorug05G0492600 Rorug05G0492700
rosa_samantha Rh6AG002400 Rh6BG007700 Rh6CG001600 Rh6DG001600
rosa_wichuraiana Rw6G000300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1841
AccB7I CCANNNNNTGG 1 cut(s) 1766
AccI GTMKAC 1 cut(s) 136
AccII CGCG 1 cut(s) 762
AciI CCGC 2 cut(s) 47, 762
AclWI GGATC 1 cut(s) 1148
AcsI RAATTY 1 cut(s) 1750
AfaI GTAC 7 cut(s) 195, 428, 733, 1066, 1126, 1236, 1802
AfiI CCNNNNNNNGG 5 cut(s) 393, 646, 761, 946, 1766
AhdI GACNNNNNGTC 1 cut(s) 987
AjnI CCWGG 2 cut(s) 945, 1776
Alw21I GWGCWC 1 cut(s) 1553
Alw26I GTCTC 3 cut(s) 691, 741, 1017
AlwI GGATC 1 cut(s) 1148
AlwNI CAGNNNCTG 1 cut(s) 84
AoxI GGCC 5 cut(s) 395, 486, 1300, 1365, 1426
ApoI RAATTY 1 cut(s) 1750
Asp700I GAANNNNTTC 1 cut(s) 846
AspA2I CCTAGG 1 cut(s) 130
AspS9I GGNCC 4 cut(s) 318, 649, 981, 1642
AsuHPI GGTGA 6 cut(s) 178, 880, 898, 1135, 1728, 1746
AsuII TTCGAA 1 cut(s) 1376
AvaII GGWCC 4 cut(s) 318, 649, 981, 1642
AvrII CCTAGG 1 cut(s) 130
AxyI CCTNAGG 1 cut(s) 490
BaeGI GKGCMC 1 cut(s) 1053
BanII GRGCYC 1 cut(s) 1553
BbsI GAAGAC 1 cut(s) 253
Bbv12I GWGCWC 1 cut(s) 1553
BccI CCATC 7 cut(s) 35, 148, 387, 962, 1076, 1190, 1760
BceAI ACGGC 1 cut(s) 152
BciT130I CCWGG 2 cut(s) 947, 1778
BciVI GTATCC 1 cut(s) 547
BcoDI GTCTC 3 cut(s) 691, 741, 1017
BfaI CTAG 3 cut(s) 131, 707, 941
BfmI CTRYAG 4 cut(s) 39, 690, 1335, 1446
BfuI GTATCC 1 cut(s) 547
BlnI CCTAGG 1 cut(s) 130
BmcAI AGTACT 1 cut(s) 428
Bme1390I CCNGG 2 cut(s) 947, 1778
Bme18I GGWCC 4 cut(s) 318, 649, 981, 1642
BmeRI GACNNNNNGTC 1 cut(s) 987
BmgT120I GGNCC 4 cut(s) 318, 649, 981, 1642
BmiI GGNNCC 2 cut(s) 287, 650
BmrFI CCNGG 2 cut(s) 947, 1778
BmsI GCATC 3 cut(s) 34, 195, 963
BpiI GAAGAC 1 cut(s) 253
BplI GAGNNNNNCTC 2 cut(s) 44, 76
Bpu10I CCTNAGC 1 cut(s) 392
Bpu14I TTCGAA 1 cut(s) 1376
BpuEI CTTGAG 1 cut(s) 771
BsaBI GATNNNNATC 1 cut(s) 300
BsaJI CCNNGG 2 cut(s) 130, 992
Bsc4I CCNNNNNNNGG 5 cut(s) 393, 646, 761, 946, 1766
Bse1I ACTGG 3 cut(s) 538, 1261, 1303
Bse21I CCTNAGG 1 cut(s) 490
Bse3DI GCAATG 1 cut(s) 624
Bse8I GATNNNNATC 1 cut(s) 300
BseBI CCWGG 2 cut(s) 947, 1778
BseDI CCNNGG 2 cut(s) 130, 992
BseGI GGATG 2 cut(s) 676, 1182
BseJI GATNNNNATC 1 cut(s) 300
BseLI CCNNNNNNNGG 5 cut(s) 393, 646, 761, 946, 1766
BseMI GCAATG 1 cut(s) 624
BseMII CTCAG 6 cut(s) 24, 966, 1028, 1056, 1538, 1684
BseNI ACTGG 3 cut(s) 538, 1261, 1303
BseRI GAGGAG 3 cut(s) 27, 257, 905
BseSI GKGCMC 1 cut(s) 1053
BseYI CCCAGC 2 cut(s) 387, 1165
Bsh1236I CGCG 1 cut(s) 762
BshFI GGCC 5 cut(s) 397, 488, 1302, 1367, 1428
BsiHKAI GWGCWC 1 cut(s) 1553
BsiSI CCGG 1 cut(s) 663
BslFI GGGAC 3 cut(s) 268, 1196, 1277
BslI CCNNNNNNNGG 5 cut(s) 393, 646, 761, 946, 1766
BsmAI GTCTC 3 cut(s) 691, 741, 1017
BsmBI CGTCTC 1 cut(s) 741
BsmFI GGGAC 3 cut(s) 268, 1196, 1277
BsmI GAATGC 2 cut(s) 1372, 1497
BsnI GGCC 5 cut(s) 397, 488, 1302, 1367, 1428
Bsp119I TTCGAA 1 cut(s) 1376
Bsp1286I GDGCHC 2 cut(s) 1053, 1553
Bsp143I GATC 2 cut(s) 1140, 1621
Bsp19I CCATGG 1 cut(s) 992
BspACI CCGC 2 cut(s) 47, 762
BspANI GGCC 5 cut(s) 397, 488, 1302, 1367, 1428
BspCNI CTCAG 6 cut(s) 25, 967, 1027, 1055, 1539, 1683
BspFNI CGCG 1 cut(s) 762
BspLI GGNNCC 2 cut(s) 287, 650
BspMAI CTGCAG 4 cut(s) 43, 694, 1339, 1450
BspPI GGATC 1 cut(s) 1148
BspT104I TTCGAA 1 cut(s) 1376
BsrDI GCAATG 1 cut(s) 624
BsrI ACTGG 3 cut(s) 538, 1261, 1303
BssECI CCNNGG 2 cut(s) 130, 992
BssMI GATC 2 cut(s) 1140, 1621
BssT1I CCWWGG 2 cut(s) 130, 992
Bst2UI CCWGG 2 cut(s) 947, 1778
Bst4CI ACNGT 8 cut(s) 219, 259, 431, 637, 1009, 1228, 1413, 1646
Bst6I CTCTTC 1 cut(s) 1703
BstAPI GCANNNNNTGC 1 cut(s) 602
BstBI TTCGAA 1 cut(s) 1376
BstC8I GCNNGC 2 cut(s) 952, 1662
BstDSI CCRYGG 1 cut(s) 992
BstENI CCTNNNNNAGG 1 cut(s) 944
BstF5I GGATG 2 cut(s) 676, 1182
BstFNI CGCG 1 cut(s) 762
BstKTI GATC 2 cut(s) 1143, 1624
BstMAI GTCTC 3 cut(s) 691, 741, 1017
BstMBI GATC 2 cut(s) 1140, 1621
BstMWI GCNNNNNNNGC 3 cut(s) 44, 164, 602
BstNI CCWGG 2 cut(s) 947, 1778
BstNSI RCATGY 1 cut(s) 954
BstSCI CCNGG 2 cut(s) 945, 1776
BstSFI CTRYAG 4 cut(s) 39, 690, 1335, 1446
BstSLI GKGCMC 1 cut(s) 1053
BstUI CGCG 1 cut(s) 762
BstV2I GAAGAC 1 cut(s) 253
Bsu36I CCTNAGG 1 cut(s) 490
BsuI GTATCC 1 cut(s) 547
BsuRI GGCC 5 cut(s) 397, 488, 1302, 1367, 1428
BtgI CCRYGG 1 cut(s) 992
BtsCI GGATG 2 cut(s) 676, 1182
BtsI GCAGTG 1 cut(s) 1443
BtsIMutI CAGTG 4 cut(s) 215, 1254, 1296, 1443
Cac8I GCNNGC 2 cut(s) 952, 1662
CaiI CAGNNNCTG 1 cut(s) 84
Cfr13I GGNCC 4 cut(s) 318, 649, 981, 1642
Csp6I GTAC 7 cut(s) 194, 427, 732, 1065, 1125, 1235, 1801
CspCI CAANNNNNGTGG 2 cut(s) 85, 120
CviQI GTAC 7 cut(s) 194, 427, 732, 1065, 1125, 1235, 1801
DpnI GATC 2 cut(s) 1142, 1623
DpnII GATC 2 cut(s) 1140, 1621
DriI GACNNNNNGTC 1 cut(s) 987
Eam1104I CTCTTC 1 cut(s) 1703
Eam1105I GACNNNNNGTC 1 cut(s) 987
EarI CTCTTC 1 cut(s) 1703
Ecl136II GAGCTC 1 cut(s) 1551
Eco130I CCWWGG 2 cut(s) 130, 992
Eco24I GRGCYC 1 cut(s) 1553
Eco32I GATATC 1 cut(s) 58
Eco47I GGWCC 4 cut(s) 318, 649, 981, 1642
Eco53kI GAGCTC 1 cut(s) 1551
Eco81I CCTNAGG 1 cut(s) 490
EcoICRI GAGCTC 1 cut(s) 1551
EcoNI CCTNNNNNAGG 1 cut(s) 944
EcoRI GAATTC 1 cut(s) 1750
EcoRII CCWGG 2 cut(s) 945, 1776
EcoRV GATATC 1 cut(s) 58
EcoT14I CCWWGG 2 cut(s) 130, 992
EcoT22I ATGCAT 1 cut(s) 956
EcoT38I GRGCYC 1 cut(s) 1553
ErhI CCWWGG 2 cut(s) 130, 992
Esp3I CGTCTC 1 cut(s) 741
FaqI GGGAC 3 cut(s) 268, 1196, 1277
FblI GTMKAC 1 cut(s) 136
FokI GGATG 2 cut(s) 683, 1169
FriOI GRGCYC 1 cut(s) 1553
FspBI CTAG 3 cut(s) 131, 707, 941
GsaI CCCAGC 2 cut(s) 391, 1169
HaeIII GGCC 5 cut(s) 397, 488, 1302, 1367, 1428
HapII CCGG 1 cut(s) 663
HindIII AAGCTT 1 cut(s) 1150
HinfI GANTC 5 cut(s) 17, 296, 684, 936, 1265
HpaII CCGG 1 cut(s) 663
HphI GGTGA 6 cut(s) 178, 880, 898, 1135, 1728, 1746
Hpy166II GTNNAC 3 cut(s) 137, 1443, 1573
Hpy188I TCNGA 8 cut(s) 34, 295, 424, 459, 747, 935, 1435, 1548
Hpy188III TCNNGA 3 cut(s) 316, 434, 544
Hpy8I GTNNAC 3 cut(s) 137, 1443, 1573
HpyAV CCTTC 6 cut(s) 106, 541, 616, 1254, 1481, 1814
HpyCH4III ACNGT 8 cut(s) 219, 259, 431, 637, 1009, 1228, 1413, 1646
HpyCH4IV ACGT 2 cut(s) 734, 903
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 164, 602
HpySE526I ACGT 2 cut(s) 734, 903
Kzo9I GATC 2 cut(s) 1140, 1621
LmnI GCTCC 5 cut(s) 309, 343, 530, 1021, 1411
LweI GCATC 3 cut(s) 34, 195, 963
MaeI CTAG 3 cut(s) 131, 707, 941
MaeII ACGT 2 cut(s) 734, 903
MaeIII GTNAC 7 cut(s) 238, 886, 904, 1252, 1579, 1613, 1718
MalI GATC 2 cut(s) 1142, 1623
MboI GATC 2 cut(s) 1140, 1621
MfeI CAATTG 2 cut(s) 589, 780
MhlI GDGCHC 2 cut(s) 1053, 1553
MluCI AATT 9 cut(s) 474, 589, 714, 780, 1035, 1083, 1690, 1708, 1750
MlyI GAGTC 2 cut(s) 693, 1259
MmeI TCCRAC 2 cut(s) 655, 1138
Mph1103I ATGCAT 1 cut(s) 956
MroXI GAANNNNTTC 1 cut(s) 846
MseI TTAA 3 cut(s) 326, 632, 1808
MslI CAYNNNNRTG 1 cut(s) 1377
MspA1I CMGCKG 2 cut(s) 695, 1334
MspI CCGG 1 cut(s) 663
MspR9I CCNGG 2 cut(s) 947, 1778
MunI CAATTG 2 cut(s) 589, 780
Mva1269I GAATGC 2 cut(s) 1372, 1497
MvaI CCWGG 2 cut(s) 947, 1778
MvnI CGCG 1 cut(s) 762
MwoI GCNNNNNNNGC 3 cut(s) 44, 164, 602
NcoI CCATGG 1 cut(s) 992
NdeII GATC 2 cut(s) 1140, 1621
NlaIV GGNNCC 2 cut(s) 287, 650
NmeAIII GCCGAG 1 cut(s) 1343
NmuCI GTSAC 5 cut(s) 238, 886, 904, 1252, 1613
NsiI ATGCAT 1 cut(s) 956
NspI RCATGY 1 cut(s) 954
NspV TTCGAA 1 cut(s) 1376
PaeI GCATGC 1 cut(s) 954
PctI GAATGC 2 cut(s) 1372, 1497
PdmI GAANNNNTTC 1 cut(s) 846
PfeI GAWTC 3 cut(s) 17, 296, 936
PflMI CCANNNNNTGG 1 cut(s) 1766
PleI GAGTC 2 cut(s) 692, 1259
PpsI GAGTC 2 cut(s) 692, 1259
PsiI TTATAA 1 cut(s) 1841
Psp124BI GAGCTC 1 cut(s) 1553
Psp6I CCWGG 2 cut(s) 945, 1776
PspFI CCCAGC 2 cut(s) 387, 1165
PspGI CCWGG 2 cut(s) 945, 1776
PspN4I GGNNCC 2 cut(s) 287, 650
PspPI GGNCC 4 cut(s) 318, 649, 981, 1642
PstI CTGCAG 4 cut(s) 43, 694, 1339, 1450
PstNI CAGNNNCTG 1 cut(s) 84
PvuII CAGCTG 2 cut(s) 695, 1334
RsaI GTAC 7 cut(s) 195, 428, 733, 1066, 1126, 1236, 1802
RsaNI GTAC 7 cut(s) 194, 427, 732, 1065, 1125, 1235, 1801
RseI CAYNNNNRTG 1 cut(s) 1377
SacI GAGCTC 1 cut(s) 1553
SaqAI TTAA 3 cut(s) 326, 632, 1808
Sau3AI GATC 2 cut(s) 1140, 1621
Sau96I GGNCC 4 cut(s) 318, 649, 981, 1642
ScaI AGTACT 1 cut(s) 428
SchI GAGTC 2 cut(s) 693, 1259
ScrFI CCNGG 2 cut(s) 947, 1778
SduI GDGCHC 2 cut(s) 1053, 1553
SfaNI GCATC 3 cut(s) 34, 195, 963
SfcI CTRYAG 4 cut(s) 39, 690, 1335, 1446
SfuI TTCGAA 1 cut(s) 1376
SinI GGWCC 4 cut(s) 318, 649, 981, 1642
SmiMI CAYNNNNRTG 1 cut(s) 1377
SmlI CTYRAG 1 cut(s) 786
SmoI CTYRAG 1 cut(s) 786
SphI GCATGC 1 cut(s) 954
Sse9I AATT 9 cut(s) 474, 589, 714, 780, 1035, 1083, 1690, 1708, 1750
SsiI CCGC 2 cut(s) 47, 762
SspI AATATT 1 cut(s) 1834
SspMI CTAG 3 cut(s) 131, 707, 941
SstI GAGCTC 1 cut(s) 1553
StyD4I CCNGG 2 cut(s) 945, 1776
StyI CCWWGG 2 cut(s) 130, 992
TaaI ACNGT 8 cut(s) 219, 259, 431, 637, 1009, 1228, 1413, 1646
TaiI ACGT 2 cut(s) 737, 906
TaqI TCGA 2 cut(s) 1246, 1376
TaqII GACCGA 1 cut(s) 998
TasI AATT 9 cut(s) 474, 589, 714, 780, 1035, 1083, 1690, 1708, 1750
TatI WGTACW 3 cut(s) 193, 426, 1124
TfiI GAWTC 3 cut(s) 17, 296, 936
Tru1I TTAA 3 cut(s) 326, 632, 1808
Tru9I TTAA 3 cut(s) 326, 632, 1808
TscAI CASTG 4 cut(s) 222, 1261, 1303, 1450
TseFI GTSAC 5 cut(s) 238, 886, 904, 1252, 1613
Tsp45I GTSAC 5 cut(s) 238, 886, 904, 1252, 1613
TspDTI ATGAA 5 cut(s) 17, 125, 839, 1145, 1395
TspRI CASTG 4 cut(s) 222, 1261, 1303, 1450
Van91I CCANNNNNTGG 1 cut(s) 1766
VpaK11BI GGWCC 4 cut(s) 318, 649, 981, 1642
XagI CCTNNNNNAGG 1 cut(s) 944
XapI RAATTY 1 cut(s) 1750
XceI RCATGY 1 cut(s) 954
XcmI CCANNNNNNNNNTGG 1 cut(s) 1321
XmaJI CCTAGG 1 cut(s) 130
XmiI GTMKAC 1 cut(s) 136
XmnI GAANNNNTTC 1 cut(s) 846
XspI CTAG 3 cut(s) 131, 707, 941
ZrmI AGTACT 1 cut(s) 428
Zsp2I ATGCAT 1 cut(s) 956
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.