Rroxscaffold_7G00217660

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0013921
Physical Location & Seq
Reverse (-)
68111262 .. 68115244
3983 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_7G00217660.1

Sequence Viewer

Length: 1200 bp
ATGAAACTGAGAGGAGATTCCATGTCCATCTCTGAGGCTGCAGATGCGGTGGGGGATATCTCTGTTGATTGGAGAGGCAGACCCTGTAAGCCAACCAAACATGGTGGAATGAAGGCTGCTGTTTTTGTCCTAGGTCTACAAGGTTTTGAGATGATGGCAATAGCAGCTGTTGGGAACAATCTCATCACCTATGTACTAAATGAGATGCACTTTCCACTGTCAAAGTCTGCAAACATAGTGACCAACTTTATAGGGACAGTCTTCCTCCTCTCCCTCTTTGGAGGGTTCCTATCAGATTCTTATCTTGGGAGCTTCTGGACCATGTTAATCTTTGGGTTTGTGGAGCTTTCTGGTTTTATACTACTCTCTGTTCAAGCCCATCTCCCCCAGTTAAGGCCACCCTCCTGCAAGGTGATGTCGTCCGAGTACTGTCAGGAGGCAAAGGGCTACAAGGCTCTCATATTCTTTGCTGCAATTTACTTGGTGGCCTTAGGAAGTGGTTGTCTAAAACCAAACATAATCTCTCATGGAGCTGACCAATTTAGGAAGGAGGATACCAAGCAATCCAAGAAGTTATCAACTTACTTCAATTGTGCATACTTTGCCTTCTGCATGGGAGAACTCATTGCATTAACTGTCCTTGTTTGGGTCCAAACACACTCCGGCATGGATGTTGGATTTGGAGTCTCTGCAGCTGCCATGACACTAGGACTAATTAGCTTGGTGTTTGGTACGTCTCTGTATCGGAACAAACCACCTCACGGAAGCATTTTCACTCCAATTGCTCAAGTTTTCGTTGCTGCATTTACGAAGAGAAAGCAAATATGCCCCTCAAATACAGAAATGCTTCATGGAAACCAAAACAATGTGCCAAACCACCTCTTTGTCACCATGTCGCCTAACGTCACCACTCTCCTCCACACCGAAAAGTTCAGATTCCTAGACAAGGCATGCATCAAAACAGAAGATGGAACTGAGAGGACCGAAAGTCCATGGAGATTATGCACCGTGTCTCAGGTGGAGCAAGTCAAAATAATTATCTCAGTTGTGCCCATTTTTGCCTGTACCATCATCTTCAACACAATTTTGGCTCAACTCCAAACATTTTCAGTCCAACAAGGAAGTACAATGAACGCAAGGATCACCGAAAGCTTCAAAATCCCCCAGCTTCACTCCAAGCCATCCCTTACATCATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

399

Amino Acids

43.84

Weight (kDa)

8.89

Isoelectric Point (pI)

37.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 105 - 388 1.3e-56 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013921)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33440
fragaria_vesca FvH4_2g00200
malus_domestica MD05G1000900.v1.1
prunus_persica Prupe.8G001900_v2.0.a1 Prupe.8G001900_v2.0.a1
pyrus_communis pycom05g00010
rosa_chinensis RchiOBHm_Chr6g0249481
rosa_laevigata RLG00000015577
rosa_multiflora Rmu_sc0002916.1_g000002
rosa_roxburghii Rroxscaffold_7G00217660
rosa_rugosa Rorug05G0492600 Rorug05G0492700
rosa_samantha Rh6AG002400 Rh6BG007700 Rh6CG001600 Rh6DG001600
rosa_wichuraiana Rw6G000300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 136
AciI CCGC 1 cut(s) 47
AclWI GGATC 1 cut(s) 1148
AfaI GTAC 5 cut(s) 195, 428, 733, 1066, 1126
AfiI CCNNNNNNNGG 4 cut(s) 393, 646, 761, 946
AgsI TTSAA 4 cut(s) 374, 589, 1078, 1156
AhdI GACNNNNNGTC 1 cut(s) 987
AluBI AGCT 8 cut(s) 167, 312, 346, 533, 695, 720, 1152, 1168
AluI AGCT 8 cut(s) 167, 312, 346, 533, 695, 720, 1152, 1168
Alw26I GTCTC 3 cut(s) 691, 741, 1017
AlwI GGATC 1 cut(s) 1148
AlwNI CAGNNNCTG 1 cut(s) 84
AoxI GGCC 2 cut(s) 395, 486
ApeKI GCWGC 7 cut(s) 38, 116, 164, 470, 692, 695, 800
Asp700I GAANNNNTTC 1 cut(s) 846
AspA2I CCTAGG 1 cut(s) 130
AspS9I GGNCC 3 cut(s) 318, 649, 981
AsuHPI GGTGA 5 cut(s) 178, 424, 880, 898, 1135
AvaII GGWCC 3 cut(s) 318, 649, 981
AvrII CCTAGG 1 cut(s) 130
AxyI CCTNAGG 1 cut(s) 490
BaeGI GKGCMC 1 cut(s) 1053
BbsI GAAGAC 1 cut(s) 253
BbvI GCAGC 7 cut(s) 25, 103, 176, 457, 682, 704, 787
BccI CCATC 6 cut(s) 35, 148, 387, 962, 1076, 1189
BciVI GTATCC 1 cut(s) 547
BcoDI GTCTC 3 cut(s) 691, 741, 1017
BfaI CTAG 3 cut(s) 131, 707, 941
BfmI CTRYAG 2 cut(s) 39, 690
BfuI GTATCC 1 cut(s) 547
BisI GCNGC 7 cut(s) 39, 117, 165, 471, 693, 696, 801
BlnI CCTAGG 1 cut(s) 130
BlsI GCNGC 7 cut(s) 40, 118, 166, 472, 694, 697, 802
BmcAI AGTACT 1 cut(s) 428
Bme18I GGWCC 3 cut(s) 318, 649, 981
BmeRI GACNNNNNGTC 1 cut(s) 987
BmgT120I GGNCC 3 cut(s) 318, 649, 981
BmiI GGNNCC 2 cut(s) 287, 650
BmrI ACTGGG 1 cut(s) 382
BmsI GCATC 3 cut(s) 34, 195, 963
BmuI ACTGGG 1 cut(s) 382
BpiI GAAGAC 1 cut(s) 253
BpuEI CTTGAG 1 cut(s) 771
BsaBI GATNNNNATC 1 cut(s) 300
BsaJI CCNNGG 2 cut(s) 130, 992
Bsc4I CCNNNNNNNGG 4 cut(s) 393, 646, 761, 946
Bse1I ACTGG 1 cut(s) 388
Bse21I CCTNAGG 1 cut(s) 490
Bse3DI GCAATG 1 cut(s) 624
Bse8I GATNNNNATC 1 cut(s) 300
BseDI CCNNGG 2 cut(s) 130, 992
BseGI GGATG 2 cut(s) 676, 1181
BseJI GATNNNNATC 1 cut(s) 300
BseLI CCNNNNNNNGG 4 cut(s) 393, 646, 761, 946
BseMI GCAATG 1 cut(s) 624
BseMII CTCAG 4 cut(s) 24, 966, 1028, 1056
BseNI ACTGG 1 cut(s) 388
BseRI GAGGAG 3 cut(s) 27, 257, 905
BseSI GKGCMC 1 cut(s) 1053
BseXI GCAGC 7 cut(s) 25, 103, 176, 457, 682, 704, 787
BseYI CCCAGC 1 cut(s) 1164
BshFI GGCC 2 cut(s) 397, 488
BsiSI CCGG 1 cut(s) 663
BslFI GGGAC 1 cut(s) 268
BslI CCNNNNNNNGG 4 cut(s) 393, 646, 761, 946
BsmAI GTCTC 3 cut(s) 691, 741, 1017
BsmBI CGTCTC 1 cut(s) 741
BsmFI GGGAC 1 cut(s) 268
BsnI GGCC 2 cut(s) 397, 488
Bsp1286I GDGCHC 1 cut(s) 1053
Bsp143I GATC 1 cut(s) 1140
Bsp19I CCATGG 1 cut(s) 992
BspACI CCGC 1 cut(s) 47
BspANI GGCC 2 cut(s) 397, 488
BspCNI CTCAG 4 cut(s) 25, 967, 1027, 1055
BspLI GGNNCC 2 cut(s) 287, 650
BspMAI CTGCAG 2 cut(s) 43, 694
BspPI GGATC 1 cut(s) 1148
BsrDI GCAATG 1 cut(s) 624
BsrI ACTGG 1 cut(s) 388
BssECI CCNNGG 2 cut(s) 130, 992
BssMI GATC 1 cut(s) 1140
BssT1I CCWWGG 2 cut(s) 130, 992
Bst4CI ACNGT 5 cut(s) 219, 259, 431, 637, 1009
Bst6I CTCTTC 1 cut(s) 806
BstAPI GCANNNNNTGC 1 cut(s) 602
BstC8I GCNNGC 1 cut(s) 952
BstDEI CTNAG 6 cut(s) 8, 33, 490, 975, 1014, 1042
BstDSI CCRYGG 1 cut(s) 992
BstENI CCTNNNNNAGG 1 cut(s) 944
BstF5I GGATG 2 cut(s) 676, 1181
BstKTI GATC 1 cut(s) 1143
BstMAI GTCTC 3 cut(s) 691, 741, 1017
BstMBI GATC 1 cut(s) 1140
BstMWI GCNNNNNNNGC 3 cut(s) 44, 164, 602
BstNSI RCATGY 1 cut(s) 954
BstSFI CTRYAG 2 cut(s) 39, 690
BstSLI GKGCMC 1 cut(s) 1053
BstV1I GCAGC 7 cut(s) 25, 103, 176, 457, 682, 704, 787
BstV2I GAAGAC 1 cut(s) 253
Bsu36I CCTNAGG 1 cut(s) 490
BsuI GTATCC 1 cut(s) 547
BsuRI GGCC 2 cut(s) 397, 488
BtgI CCRYGG 1 cut(s) 992
BtsCI GGATG 2 cut(s) 676, 1181
BtsIMutI CAGTG 1 cut(s) 215
Cac8I GCNNGC 1 cut(s) 952
CaiI CAGNNNCTG 1 cut(s) 84
Cfr13I GGNCC 3 cut(s) 318, 649, 981
Csp6I GTAC 5 cut(s) 194, 427, 732, 1065, 1125
CspCI CAANNNNNGTGG 2 cut(s) 85, 120
CviQI GTAC 5 cut(s) 194, 427, 732, 1065, 1125
DdeI CTNAG 6 cut(s) 8, 33, 490, 975, 1014, 1042
DpnI GATC 1 cut(s) 1142
DpnII GATC 1 cut(s) 1140
DriI GACNNNNNGTC 1 cut(s) 987
Eam1104I CTCTTC 1 cut(s) 806
Eam1105I GACNNNNNGTC 1 cut(s) 987
EarI CTCTTC 1 cut(s) 806
Eco130I CCWWGG 2 cut(s) 130, 992
Eco32I GATATC 1 cut(s) 58
Eco47I GGWCC 3 cut(s) 318, 649, 981
Eco81I CCTNAGG 1 cut(s) 490
EcoNI CCTNNNNNAGG 1 cut(s) 944
EcoRV GATATC 1 cut(s) 58
EcoT14I CCWWGG 2 cut(s) 130, 992
EcoT22I ATGCAT 1 cut(s) 956
ErhI CCWWGG 2 cut(s) 130, 992
Esp3I CGTCTC 1 cut(s) 741
FaqI GGGAC 1 cut(s) 268
FblI GTMKAC 1 cut(s) 136
Fnu4HI GCNGC 7 cut(s) 39, 117, 165, 471, 693, 696, 801
FokI GGATG 2 cut(s) 683, 1168
Fsp4HI GCNGC 7 cut(s) 39, 117, 165, 471, 693, 696, 801
FspBI CTAG 3 cut(s) 131, 707, 941
GluI GCNGC 7 cut(s) 39, 117, 165, 471, 693, 696, 801
GsaI CCCAGC 1 cut(s) 1168
HaeIII GGCC 2 cut(s) 397, 488
HapII CCGG 1 cut(s) 663
HindIII AAGCTT 1 cut(s) 1150
HinfI GANTC 4 cut(s) 17, 296, 684, 936
HpaII CCGG 1 cut(s) 663
HphI GGTGA 5 cut(s) 178, 424, 880, 898, 1135
Hpy166II GTNNAC 1 cut(s) 137
Hpy188I TCNGA 5 cut(s) 34, 295, 424, 747, 935
Hpy188III TCNNGA 2 cut(s) 316, 434
Hpy8I GTNNAC 1 cut(s) 137
HpyAV CCTTC 3 cut(s) 106, 541, 616
HpyCH4III ACNGT 5 cut(s) 219, 259, 431, 637, 1009
HpyCH4IV ACGT 2 cut(s) 734, 903
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 164, 602
HpyF3I CTNAG 6 cut(s) 8, 33, 490, 975, 1014, 1042
HpySE526I ACGT 2 cut(s) 734, 903
Kzo9I GATC 1 cut(s) 1140
LmnI GCTCC 4 cut(s) 309, 343, 530, 1021
Lsp1109I GCAGC 7 cut(s) 25, 103, 176, 457, 682, 704, 787
LweI GCATC 3 cut(s) 34, 195, 963
MaeI CTAG 3 cut(s) 131, 707, 941
MaeII ACGT 2 cut(s) 734, 903
MaeIII GTNAC 3 cut(s) 238, 886, 904
MalI GATC 1 cut(s) 1142
MboI GATC 1 cut(s) 1140
MboII GAAGA 4 cut(s) 253, 823, 977, 1066
MfeI CAATTG 2 cut(s) 589, 780
MhlI GDGCHC 1 cut(s) 1053
MluCI AATT 7 cut(s) 474, 539, 589, 714, 780, 1035, 1083
MlyI GAGTC 1 cut(s) 693
MmeI TCCRAC 2 cut(s) 655, 1138
Mph1103I ATGCAT 1 cut(s) 956
MroXI GAANNNNTTC 1 cut(s) 846
MseI TTAA 3 cut(s) 326, 392, 632
MspA1I CMGCKG 2 cut(s) 167, 695
MspI CCGG 1 cut(s) 663
MunI CAATTG 2 cut(s) 589, 780
MwoI GCNNNNNNNGC 3 cut(s) 44, 164, 602
NcoI CCATGG 1 cut(s) 992
NdeII GATC 1 cut(s) 1140
NlaIV GGNNCC 2 cut(s) 287, 650
NmuCI GTSAC 3 cut(s) 238, 886, 904
NsiI ATGCAT 1 cut(s) 956
NspI RCATGY 1 cut(s) 954
PaeI GCATGC 1 cut(s) 954
PdmI GAANNNNTTC 1 cut(s) 846
PfeI GAWTC 3 cut(s) 17, 296, 936
PkrI GCNGC 7 cut(s) 40, 118, 166, 472, 694, 697, 802
PleI GAGTC 1 cut(s) 692
PpsI GAGTC 1 cut(s) 692
PspFI CCCAGC 1 cut(s) 1164
PspN4I GGNNCC 2 cut(s) 287, 650
PspPI GGNCC 3 cut(s) 318, 649, 981
PstI CTGCAG 2 cut(s) 43, 694
PstNI CAGNNNCTG 1 cut(s) 84
PvuII CAGCTG 2 cut(s) 167, 695
RsaI GTAC 5 cut(s) 195, 428, 733, 1066, 1126
RsaNI GTAC 5 cut(s) 194, 427, 732, 1065, 1125
SaqAI TTAA 3 cut(s) 326, 392, 632
SatI GCNGC 7 cut(s) 39, 117, 165, 471, 693, 696, 801
Sau3AI GATC 1 cut(s) 1140
Sau96I GGNCC 3 cut(s) 318, 649, 981
ScaI AGTACT 1 cut(s) 428
SchI GAGTC 1 cut(s) 693
SduI GDGCHC 1 cut(s) 1053
SfaNI GCATC 3 cut(s) 34, 195, 963
SfcI CTRYAG 2 cut(s) 39, 690
SinI GGWCC 3 cut(s) 318, 649, 981
SmlI CTYRAG 1 cut(s) 786
SmoI CTYRAG 1 cut(s) 786
SphI GCATGC 1 cut(s) 954
Sse9I AATT 7 cut(s) 474, 539, 589, 714, 780, 1035, 1083
SsiI CCGC 1 cut(s) 47
SspMI CTAG 3 cut(s) 131, 707, 941
StyI CCWWGG 2 cut(s) 130, 992
TaaI ACNGT 5 cut(s) 219, 259, 431, 637, 1009
TaiI ACGT 2 cut(s) 737, 906
TaqII GACCGA 1 cut(s) 998
TasI AATT 7 cut(s) 474, 539, 589, 714, 780, 1035, 1083
TatI WGTACW 3 cut(s) 193, 426, 1124
TfiI GAWTC 3 cut(s) 17, 296, 936
Tru1I TTAA 3 cut(s) 326, 392, 632
Tru9I TTAA 3 cut(s) 326, 392, 632
TscAI CASTG 1 cut(s) 222
TseFI GTSAC 3 cut(s) 238, 886, 904
TseI GCWGC 7 cut(s) 38, 116, 164, 470, 692, 695, 800
Tsp45I GTSAC 3 cut(s) 238, 886, 904
TspDTI ATGAA 4 cut(s) 17, 125, 839, 1145
TspGWI ACGGA 1 cut(s) 777
TspRI CASTG 1 cut(s) 222
VpaK11BI GGWCC 3 cut(s) 318, 649, 981
XagI CCTNNNNNAGG 1 cut(s) 944
XceI RCATGY 1 cut(s) 954
XmaJI CCTAGG 1 cut(s) 130
XmiI GTMKAC 1 cut(s) 136
XmnI GAANNNNTTC 1 cut(s) 846
XspI CTAG 3 cut(s) 131, 707, 941
ZrmI AGTACT 1 cut(s) 428
Zsp2I ATGCAT 1 cut(s) 956
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.