Rh6DG001600

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0013921
Physical Location & Seq
Forward (+)
286827 .. 290521
3695 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG001600.1

Sequence Viewer

Length: 1824 bp
ATGTCCATCTCTGAGGCTGCAGATGCGGTGGAGGATATCTCTGTTGATTGGAGAGGCAGACCCTGTAAGCCAACCAAATATGGTGGAATGAAGGCTGCTGTTTTTGTCCTAGGTCTACAAGGTTTTGAGATGATGGCAATAGCAGCTGTTGGGAACAATCTCATCACCTATGTACTAAATGAGATGCACTTTCCACTGTCAAAGTCTGCAAACATAGTGACCAACTTTATAGGGACAGTCTTCCTCCTCTCCCTCTTTGGAGGGTTCCTATCAGATTCTTATCTTGGGAGCTTCTGGACCATGTTAATCTTTGGGTTTGTGGAGCTTTCTGGTTTTATACTACTCTCTGTTCAAGCCCATCTCCCCCAGCTAAGGCCACCCTCCTGCAAGGTTATGTCGTCCGAGTACTGTCAGGAGGCAAAGGGTTACAAGGCTCTCATATTCTTTGCTGCAATTTACTTGGTGGCCTTAGGAAGTGGTTGTCTAAAACCAAACATAATCTCTCATGGAGCTGACCAATTCAGGAAGGAGGATACCGAGCAATCCAAGAAGTTATCAACTTACTTCAATTGTGCATACTTTGCCTTCTGCATGGGAGAACTCATTGCATTAACTGTCCTTGTTTGGGTCCAAACACACTCCGGCATGGATGTTGGATTTGGAGTCTCTGCAGCTGCCATGACACTAGGACTAATTAGCTTGGTGTTTGGTACGTCTCTGTATCGGAACAAACCACCTCGCGGAAGCATTTTCACTCCAATTGCTCAAGTTTTCGTTGCTGCATTTACAAAGAGAAAGCAAATATGCCCCTCAAATACAGAAATGCTTCATGGAAACCAAAACAATGTGCCAAACCACCTCTTTGTCACCATGTCGCCTAACGTCACCACTCTCCTCCACACCGAAAAGTTCAGATTCCTAGACAAGGCATGCATCAAAACAGAAGATGGAACTGAGAGGACCGAAACTCCATGGAGATTATGCACCGTGTCTCAGGTGGAGCAAGTCAAAATAATTATCTCAGTAGTGCCCATTTTTGCCTGTACCATCATCTTCAACACAATTTTGGCTCAACTTCAAACATTTTCAGTCCAACAAGGAAGTACAATGAACGCAAGGATCACTGAAAGCTTCAAAATCCCCCCAGCTTCACTCCAAGCTATCCCTTACATCATGCTAATCTTTCTTGTCCCTCTCTACGAAACCGTCTTTGTACCGACTGCTCGAAAAGTCACCGGAAGGGACTCTGGGATTTCTCCTTTACAAAGAGTTGGCACTGGCCTTTTTATTGCCACCTTTTCAATGGTTTCAGCTGCAGTTATTGAGAGAAAGAGAAGAAACTCGGCCTTGCACAATGAAGCTCTTTCAATCTTTTGGATTGCTCCACAGTTTCTCATCTTCGGCCTGTCAGAGATGTTCACTGCAGTAGGGCTTATAGAGTTCTTCTACAAGCAGTCATTGGAAGGAATGCAATCATTTCTAACTGCCATGACATACTGCTCATACTCATTTGGCTTCTATTTGAGCTCACTCCTTGTTTCTCTTGTGAACAAAGTTACCTCAACTTCTTCAAGGGGAGGGTGGCTCAGTGACAATGATCTCAACAAGGATAGGCTGGACCGTTTCTATTGGCTGCTGGCTGCTCTCAGTCTTATCAACTTCTTCAATTATCTCTTCTGGTCAAATTGGTATTGTTACAACCCCACTCTATCACCCACTAATACTAATTCACCCCCAGAAGATGGAAAAGACCTGGAAAACAGCAGCAACTTCAAGTACCCTTCAAGGCATATTGAAGGTTCTCATAATATTATTATAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

607

Amino Acids

67.6

Weight (kDa)

8.47

Isoelectric Point (pI)

42.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 98 - 528 3.9e-92 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013921)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33440
fragaria_vesca FvH4_2g00200
malus_domestica MD05G1000900.v1.1
prunus_persica Prupe.8G001900_v2.0.a1 Prupe.8G001900_v2.0.a1
pyrus_communis pycom05g00010
rosa_chinensis RchiOBHm_Chr6g0249481
rosa_laevigata RLG00000015577
rosa_multiflora Rmu_sc0002916.1_g000002
rosa_roxburghii Rroxscaffold_7G00217660
rosa_rugosa Rorug05G0492600 Rorug05G0492700
rosa_samantha Rh6AG002400 Rh6BG007700 Rh6CG001600 Rh6DG001600
rosa_wichuraiana Rw6G000300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1817
AccB7I CCANNNNNTGG 1 cut(s) 1742
AccI GTMKAC 1 cut(s) 115
AccII CGCG 1 cut(s) 741
AciI CCGC 2 cut(s) 26, 741
AclWI GGATC 1 cut(s) 1127
AfaI GTAC 7 cut(s) 174, 407, 712, 1045, 1105, 1215, 1778
AfiI CCNNNNNNNGG 5 cut(s) 372, 625, 740, 925, 1742
AjnI CCWGG 1 cut(s) 1752
Alw21I GWGCWC 1 cut(s) 1529
Alw26I GTCTC 3 cut(s) 670, 720, 996
AlwI GGATC 1 cut(s) 1127
AlwNI CAGNNNCTG 1 cut(s) 63
AoxI GGCC 5 cut(s) 374, 465, 1279, 1344, 1402
Asp700I GAANNNNTTC 1 cut(s) 825
AspA2I CCTAGG 1 cut(s) 109
AspS9I GGNCC 4 cut(s) 297, 628, 960, 1618
AsuHPI GGTGA 6 cut(s) 157, 859, 877, 1225, 1704, 1722
AvaII GGWCC 4 cut(s) 297, 628, 960, 1618
AvrII CCTAGG 1 cut(s) 109
AxyI CCTNAGG 1 cut(s) 469
BaeGI GKGCMC 1 cut(s) 1032
BanII GRGCYC 1 cut(s) 1529
BbsI GAAGAC 1 cut(s) 232
Bbv12I GWGCWC 1 cut(s) 1529
BccI CCATC 6 cut(s) 14, 127, 366, 941, 1055, 1736
BciT130I CCWGG 1 cut(s) 1754
BciVI GTATCC 1 cut(s) 526
BcoDI GTCTC 3 cut(s) 670, 720, 996
BfaI CTAG 3 cut(s) 110, 686, 920
BfmI CTRYAG 4 cut(s) 18, 669, 1314, 1422
BfuI GTATCC 1 cut(s) 526
BlnI CCTAGG 1 cut(s) 109
BmcAI AGTACT 1 cut(s) 407
Bme1390I CCNGG 1 cut(s) 1754
Bme18I GGWCC 4 cut(s) 297, 628, 960, 1618
BmgT120I GGNCC 4 cut(s) 297, 628, 960, 1618
BmiI GGNNCC 2 cut(s) 266, 629
BmrFI CCNGG 1 cut(s) 1754
BmsI GCATC 3 cut(s) 13, 174, 942
BpiI GAAGAC 1 cut(s) 232
BplI GAGNNNNNCTC 4 cut(s) 23, 55, 1569, 1601
Bpu10I CCTNAGC 1 cut(s) 371
BpuEI CTTGAG 1 cut(s) 750
BsaBI GATNNNNATC 1 cut(s) 279
BsaJI CCNNGG 2 cut(s) 109, 971
BsaWI WCCGGW 1 cut(s) 1235
BsaXI ACNNNNNCTCC 2 cut(s) 952, 982
Bsc4I CCNNNNNNNGG 5 cut(s) 372, 625, 740, 925, 1742
Bse1I ACTGG 1 cut(s) 1282
Bse21I CCTNAGG 1 cut(s) 469
Bse3DI GCAATG 1 cut(s) 603
Bse8I GATNNNNATC 1 cut(s) 279
BseBI CCWGG 1 cut(s) 1754
BseDI CCNNGG 2 cut(s) 109, 971
BseGI GGATG 1 cut(s) 655
BseJI GATNNNNATC 1 cut(s) 279
BseLI CCNNNNNNNGG 5 cut(s) 372, 625, 740, 925, 1742
BseMI GCAATG 1 cut(s) 603
BseMII CTCAG 5 cut(s) 945, 1007, 1035, 1600, 1660
BseNI ACTGG 1 cut(s) 1282
BseRI GAGGAG 2 cut(s) 236, 884
BseSI GKGCMC 1 cut(s) 1032
BseYI CCCAGC 2 cut(s) 366, 1144
Bsh1236I CGCG 1 cut(s) 741
BshFI GGCC 5 cut(s) 376, 467, 1281, 1346, 1404
BsiHKAI GWGCWC 1 cut(s) 1529
BsiSI CCGG 2 cut(s) 642, 1236
BslFI GGGAC 3 cut(s) 247, 1175, 1256
BslI CCNNNNNNNGG 5 cut(s) 372, 625, 740, 925, 1742
BsmAI GTCTC 3 cut(s) 670, 720, 996
BsmBI CGTCTC 1 cut(s) 720
BsmFI GGGAC 3 cut(s) 247, 1175, 1256
BsmI GAATGC 1 cut(s) 1473
BsnI GGCC 5 cut(s) 376, 467, 1281, 1346, 1404
Bsp1286I GDGCHC 2 cut(s) 1032, 1529
Bsp143I GATC 2 cut(s) 1119, 1597
Bsp19I CCATGG 1 cut(s) 971
BspACI CCGC 2 cut(s) 26, 741
BspANI GGCC 5 cut(s) 376, 467, 1281, 1346, 1404
BspCNI CTCAG 6 cut(s) 4, 946, 1006, 1034, 1599, 1659
BspFNI CGCG 1 cut(s) 741
BspLI GGNNCC 2 cut(s) 266, 629
BspMAI CTGCAG 4 cut(s) 22, 673, 1318, 1426
BspPI GGATC 1 cut(s) 1127
BsrDI GCAATG 1 cut(s) 603
BsrI ACTGG 1 cut(s) 1282
BssECI CCNNGG 2 cut(s) 109, 971
BssMI GATC 2 cut(s) 1119, 1597
BssT1I CCWWGG 2 cut(s) 109, 971
Bst2UI CCWGG 1 cut(s) 1754
Bst4CI ACNGT 8 cut(s) 198, 238, 410, 616, 988, 1207, 1389, 1622
Bst6I CTCTTC 1 cut(s) 1679
BstAPI GCANNNNNTGC 1 cut(s) 581
BstC8I GCNNGC 2 cut(s) 931, 1638
BstDEI CTNAG 8 cut(s) 12, 371, 469, 954, 993, 1021, 1586, 1646
BstDSI CCRYGG 1 cut(s) 971
BstENI CCTNNNNNAGG 1 cut(s) 923
BstF5I GGATG 1 cut(s) 655
BstFNI CGCG 1 cut(s) 741
BstKTI GATC 2 cut(s) 1122, 1600
BstMAI GTCTC 3 cut(s) 670, 720, 996
BstMBI GATC 2 cut(s) 1119, 1597
BstMWI GCNNNNNNNGC 3 cut(s) 23, 143, 581
BstNI CCWGG 1 cut(s) 1754
BstNSI RCATGY 1 cut(s) 933
BstSCI CCNGG 1 cut(s) 1752
BstSFI CTRYAG 4 cut(s) 18, 669, 1314, 1422
BstSLI GKGCMC 1 cut(s) 1032
BstUI CGCG 1 cut(s) 741
BstV2I GAAGAC 1 cut(s) 232
Bsu36I CCTNAGG 1 cut(s) 469
BsuI GTATCC 1 cut(s) 526
BsuRI GGCC 5 cut(s) 376, 467, 1281, 1346, 1404
BtgI CCRYGG 1 cut(s) 971
BtsCI GGATG 1 cut(s) 655
BtsI GCAGTG 1 cut(s) 1419
BtsIMutI CAGTG 5 cut(s) 194, 1122, 1275, 1419, 1594
Cac8I GCNNGC 2 cut(s) 931, 1638
CaiI CAGNNNCTG 1 cut(s) 63
Cfr13I GGNCC 4 cut(s) 297, 628, 960, 1618
Csp6I GTAC 7 cut(s) 173, 406, 711, 1044, 1104, 1214, 1777
CspCI CAANNNNNGTGG 2 cut(s) 64, 99
CviQI GTAC 7 cut(s) 173, 406, 711, 1044, 1104, 1214, 1777
DdeI CTNAG 8 cut(s) 12, 371, 469, 954, 993, 1021, 1586, 1646
DpnI GATC 2 cut(s) 1121, 1599
DpnII GATC 2 cut(s) 1119, 1597
Eam1104I CTCTTC 1 cut(s) 1679
EarI CTCTTC 1 cut(s) 1679
Ecl136II GAGCTC 1 cut(s) 1527
Eco130I CCWWGG 2 cut(s) 109, 971
Eco24I GRGCYC 1 cut(s) 1529
Eco32I GATATC 1 cut(s) 37
Eco47I GGWCC 4 cut(s) 297, 628, 960, 1618
Eco53kI GAGCTC 1 cut(s) 1527
Eco81I CCTNAGG 1 cut(s) 469
EcoICRI GAGCTC 1 cut(s) 1527
EcoNI CCTNNNNNAGG 1 cut(s) 923
EcoRII CCWGG 1 cut(s) 1752
EcoRV GATATC 1 cut(s) 37
EcoT14I CCWWGG 2 cut(s) 109, 971
EcoT22I ATGCAT 1 cut(s) 935
EcoT38I GRGCYC 1 cut(s) 1529
ErhI CCWWGG 2 cut(s) 109, 971
Esp3I CGTCTC 1 cut(s) 720
FaqI GGGAC 3 cut(s) 247, 1175, 1256
FblI GTMKAC 1 cut(s) 115
FokI GGATG 1 cut(s) 662
FriOI GRGCYC 1 cut(s) 1529
FspBI CTAG 3 cut(s) 110, 686, 920
GsaI CCCAGC 2 cut(s) 370, 1148
HaeIII GGCC 5 cut(s) 376, 467, 1281, 1346, 1404
HapII CCGG 2 cut(s) 642, 1236
HindIII AAGCTT 1 cut(s) 1129
HinfI GANTC 4 cut(s) 275, 663, 915, 1244
HpaII CCGG 2 cut(s) 642, 1236
HphI GGTGA 6 cut(s) 157, 859, 877, 1225, 1704, 1722
Hpy166II GTNNAC 3 cut(s) 116, 1419, 1549
Hpy188I TCNGA 6 cut(s) 13, 274, 403, 726, 914, 1411
Hpy188III TCNNGA 3 cut(s) 295, 413, 523
Hpy8I GTNNAC 3 cut(s) 116, 1419, 1549
HpyAV CCTTC 7 cut(s) 85, 520, 595, 1233, 1457, 1790, 1791
HpyCH4III ACNGT 8 cut(s) 198, 238, 410, 616, 988, 1207, 1389, 1622
HpyCH4IV ACGT 2 cut(s) 713, 882
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 143, 581
HpyF3I CTNAG 8 cut(s) 12, 371, 469, 954, 993, 1021, 1586, 1646
HpySE526I ACGT 2 cut(s) 713, 882
Kzo9I GATC 2 cut(s) 1119, 1597
LmnI GCTCC 5 cut(s) 288, 322, 509, 1000, 1387
LweI GCATC 3 cut(s) 13, 174, 942
MaeI CTAG 3 cut(s) 110, 686, 920
MaeII ACGT 2 cut(s) 713, 882
MaeIII GTNAC 8 cut(s) 217, 425, 865, 883, 1231, 1555, 1589, 1694
MalI GATC 2 cut(s) 1121, 1599
MboI GATC 2 cut(s) 1119, 1597
MfeI CAATTG 2 cut(s) 568, 759
MhlI GDGCHC 2 cut(s) 1032, 1529
MlyI GAGTC 2 cut(s) 672, 1238
MmeI TCCRAC 2 cut(s) 634, 1117
Mph1103I ATGCAT 1 cut(s) 935
MroXI GAANNNNTTC 1 cut(s) 825
MseI TTAA 2 cut(s) 305, 611
MspA1I CMGCKG 3 cut(s) 146, 674, 1313
MspI CCGG 2 cut(s) 642, 1236
MspR9I CCNGG 1 cut(s) 1754
MunI CAATTG 2 cut(s) 568, 759
Mva1269I GAATGC 1 cut(s) 1473
MvaI CCWGG 1 cut(s) 1754
MvnI CGCG 1 cut(s) 741
MwoI GCNNNNNNNGC 3 cut(s) 23, 143, 581
NcoI CCATGG 1 cut(s) 971
NdeII GATC 2 cut(s) 1119, 1597
NlaIV GGNNCC 2 cut(s) 266, 629
NmeAIII GCCGAG 1 cut(s) 1322
NmuCI GTSAC 5 cut(s) 217, 865, 883, 1231, 1589
NsiI ATGCAT 1 cut(s) 935
NspI RCATGY 1 cut(s) 933
PaeI GCATGC 1 cut(s) 933
PctI GAATGC 1 cut(s) 1473
PdmI GAANNNNTTC 1 cut(s) 825
PfeI GAWTC 2 cut(s) 275, 915
PflMI CCANNNNNTGG 1 cut(s) 1742
PleI GAGTC 2 cut(s) 671, 1238
PpsI GAGTC 2 cut(s) 671, 1238
PsiI TTATAA 1 cut(s) 1817
Psp124BI GAGCTC 1 cut(s) 1529
Psp6I CCWGG 1 cut(s) 1752
PspFI CCCAGC 2 cut(s) 366, 1144
PspGI CCWGG 1 cut(s) 1752
PspN4I GGNNCC 2 cut(s) 266, 629
PspPI GGNCC 4 cut(s) 297, 628, 960, 1618
PstI CTGCAG 4 cut(s) 22, 673, 1318, 1426
PstNI CAGNNNCTG 1 cut(s) 63
PvuII CAGCTG 3 cut(s) 146, 674, 1313
RsaI GTAC 7 cut(s) 174, 407, 712, 1045, 1105, 1215, 1778
RsaNI GTAC 7 cut(s) 173, 406, 711, 1044, 1104, 1214, 1777
SacI GAGCTC 1 cut(s) 1529
SaqAI TTAA 2 cut(s) 305, 611
Sau3AI GATC 2 cut(s) 1119, 1597
Sau96I GGNCC 4 cut(s) 297, 628, 960, 1618
ScaI AGTACT 1 cut(s) 407
SchI GAGTC 2 cut(s) 672, 1238
ScrFI CCNGG 1 cut(s) 1754
SduI GDGCHC 2 cut(s) 1032, 1529
SfaNI GCATC 3 cut(s) 13, 174, 942
SfcI CTRYAG 4 cut(s) 18, 669, 1314, 1422
SinI GGWCC 4 cut(s) 297, 628, 960, 1618
SmlI CTYRAG 1 cut(s) 765
SmoI CTYRAG 1 cut(s) 765
SphI GCATGC 1 cut(s) 933
SsiI CCGC 2 cut(s) 26, 741
SspI AATATT 1 cut(s) 1810
SspMI CTAG 3 cut(s) 110, 686, 920
SstI GAGCTC 1 cut(s) 1529
StyD4I CCNGG 1 cut(s) 1752
StyI CCWWGG 2 cut(s) 109, 971
TaaI ACNGT 8 cut(s) 198, 238, 410, 616, 988, 1207, 1389, 1622
TaiI ACGT 2 cut(s) 716, 885
TaqI TCGA 1 cut(s) 1225
TaqII GACCGA 1 cut(s) 977
TatI WGTACW 3 cut(s) 172, 405, 1103
TfiI GAWTC 2 cut(s) 275, 915
Tru1I TTAA 2 cut(s) 305, 611
Tru9I TTAA 2 cut(s) 305, 611
TscAI CASTG 5 cut(s) 201, 1129, 1282, 1426, 1594
TseFI GTSAC 5 cut(s) 217, 865, 883, 1231, 1589
Tsp45I GTSAC 5 cut(s) 217, 865, 883, 1231, 1589
TspDTI ATGAA 4 cut(s) 104, 818, 1124, 1371
TspRI CASTG 5 cut(s) 201, 1129, 1282, 1426, 1594
Van91I CCANNNNNTGG 1 cut(s) 1742
VpaK11BI GGWCC 4 cut(s) 297, 628, 960, 1618
XagI CCTNNNNNAGG 1 cut(s) 923
XceI RCATGY 1 cut(s) 933
XcmI CCANNNNNNNNNTGG 1 cut(s) 1300
XmaJI CCTAGG 1 cut(s) 109
XmiI GTMKAC 1 cut(s) 115
XmnI GAANNNNTTC 1 cut(s) 825
XspI CTAG 3 cut(s) 110, 686, 920
ZrmI AGTACT 1 cut(s) 407
Zsp2I ATGCAT 1 cut(s) 935
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.