AT1G33490

Uncharacterized protein conserved in bacteria (DUF2062)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007285
Physical Location & Seq
Reverse (-)
12150674 .. 12152460
1787 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G33490.1

Sequence Viewer

Length: 528 bp
ATGGCGATTTCGAAAGGTAGATTGAAAGTTTGGTTCGATAAGAAGATCAGTGAACCACTTATCCAGATCTTGCGCAGAGGTGCTGAGCCAAAGCAGCTGGCTTTTTCTGCGGCATTGGGTATTACCATGGGAGTATTTCCAATCTGTGGTGTTACGGTGTTACTATGTGGAGTGGCTATTGCGTCGCTTGGATCTCTTTGTCATGCTCCAACTGTGATGTTGGCTAACTTCATTGCTACTCCAATAGAACTAAGTCTTGTGGTGCCTTTCTTACGACTTGGCGAAAAAATAACCGGTGGACCTCATTTTCCTTTAACATCTGATGCCCTAAAGAAGGTTTTCACAGGCCAAGCTTCCCGAGATGTTTTTTTAAGCATTGGAAATGCGCTACTAGGATGGCTCATAGCGGCACCATTTGTGATCGTTGCACTATACATCATGTTTCTCCCATGTTTCAAAATACTTGTCCGCAAATTCAGCTCTGTTGATTCAACCGCAAAGACACCTACAAAGGAAAGGATCGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2062

Protein Analysis

175

Amino Acids

18.83

Weight (kDa)

9.86

Isoelectric Point (pI)

30.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2062 PF09835 19 - 158 5.7e-08 Uncharacterized protein conserved in bacteria (DUF2062)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 74
AccB1I GGYRCC 2 cut(s) 262, 409
AccB7I CCANNNNNTGG 1 cut(s) 146
AccII CGCG 1 cut(s) 524
AciI CCGC 4 cut(s) 110, 407, 469, 495
AclWI GGATC 2 cut(s) 199, 527
AcsI RAATTY 1 cut(s) 473
AfiI CCNNNNNNNGG 2 cut(s) 146, 334
AgeI ACCGGT 1 cut(s) 293
AgsI TTSAA 3 cut(s) 25, 457, 492
AloI GAACNNNNNNTCC 2 cut(s) 45, 77
AluBI AGCT 3 cut(s) 97, 353, 480
AluI AGCT 3 cut(s) 97, 353, 480
AlwI GGATC 2 cut(s) 199, 527
Ama87I CYCGRG 1 cut(s) 357
AoxI GGCC 1 cut(s) 346
ApeKI GCWGC 1 cut(s) 94
ApoI RAATTY 1 cut(s) 473
ArsI GACNNNNNNTTYG 2 cut(s) 450, 482
AsiGI ACCGGT 1 cut(s) 293
Asp700I GAANNNNTTC 1 cut(s) 338
AspLEI GCGC 2 cut(s) 75, 388
AspS9I GGNCC 1 cut(s) 299
AsuII TTCGAA 1 cut(s) 11
AvaI CYCGRG 1 cut(s) 357
AvaII GGWCC 1 cut(s) 299
BanI GGYRCC 2 cut(s) 262, 409
BbvI GCAGC 1 cut(s) 106
BccI CCATC 1 cut(s) 390
BfaI CTAG 1 cut(s) 392
BglII AGATCT 1 cut(s) 66
BisI GCNGC 3 cut(s) 95, 111, 408
BlpI GCTNAGC 1 cut(s) 84
BlsI GCNGC 3 cut(s) 96, 112, 409
Bme18I GGWCC 1 cut(s) 299
BmeT110I CYCGRG 1 cut(s) 357
BmgT120I GGNCC 1 cut(s) 299
BmiI GGNNCC 2 cut(s) 264, 411
BmsI GCATC 1 cut(s) 313
Bpu1102I GCTNAGC 1 cut(s) 84
Bpu14I TTCGAA 1 cut(s) 11
BsaJI CCNNGG 1 cut(s) 126
BsaWI WCCGGW 1 cut(s) 293
Bsc4I CCNNNNNNNGG 2 cut(s) 146, 334
Bse118I RCCGGY 1 cut(s) 293
Bse3DI GCAATG 1 cut(s) 231
BseDI CCNNGG 1 cut(s) 126
BseGI GGATG 1 cut(s) 401
BseLI CCNNNNNNNGG 2 cut(s) 146, 334
BseMI GCAATG 1 cut(s) 231
BseMII CTCAG 1 cut(s) 75
BseXI GCAGC 1 cut(s) 106
Bsh1236I CGCG 1 cut(s) 524
BshFI GGCC 1 cut(s) 348
BshNI GGYRCC 2 cut(s) 262, 409
BshTI ACCGGT 1 cut(s) 293
BsiHKCI CYCGRG 1 cut(s) 357
BsiSI CCGG 1 cut(s) 294
BslI CCNNNNNNNGG 2 cut(s) 146, 334
BsnI GGCC 1 cut(s) 348
BsoBI CYCGRG 1 cut(s) 357
Bsp119I TTCGAA 1 cut(s) 11
Bsp143I GATC 5 cut(s) 45, 66, 191, 420, 519
Bsp1720I GCTNAGC 1 cut(s) 84
Bsp19I CCATGG 1 cut(s) 126
BspACI CCGC 4 cut(s) 110, 407, 469, 495
BspANI GGCC 1 cut(s) 348
BspCNI CTCAG 1 cut(s) 76
BspFNI CGCG 1 cut(s) 524
BspLI GGNNCC 2 cut(s) 264, 411
BspPI GGATC 2 cut(s) 199, 527
BspT104I TTCGAA 1 cut(s) 11
BspT107I GGYRCC 2 cut(s) 262, 409
BsrDI GCAATG 1 cut(s) 231
BsrFI RCCGGY 1 cut(s) 293
BssAI RCCGGY 1 cut(s) 293
BssECI CCNNGG 1 cut(s) 126
BssMI GATC 5 cut(s) 45, 66, 191, 420, 519
BssT1I CCWWGG 1 cut(s) 126
Bst4CI ACNGT 2 cut(s) 157, 214
BstBI TTCGAA 1 cut(s) 11
BstC8I GCNNGC 1 cut(s) 99
BstDEI CTNAG 2 cut(s) 84, 251
BstDSI CCRYGG 1 cut(s) 126
BstENI CCTNNNNNAGG 1 cut(s) 332
BstF5I GGATG 1 cut(s) 401
BstFNI CGCG 1 cut(s) 524
BstHHI GCGC 2 cut(s) 75, 388
BstKTI GATC 5 cut(s) 48, 69, 194, 423, 522
BstMBI GATC 5 cut(s) 45, 66, 191, 420, 519
BstMWI GCNNNNNNNGC 3 cut(s) 94, 107, 477
BstUI CGCG 1 cut(s) 524
BstV1I GCAGC 1 cut(s) 106
BstX2I RGATCY 2 cut(s) 66, 191
BstYI RGATCY 2 cut(s) 66, 191
BsuRI GGCC 1 cut(s) 348
BtgI CCRYGG 1 cut(s) 126
BtsCI GGATG 1 cut(s) 401
BtsIMutI CAGTG 1 cut(s) 55
Cac8I GCNNGC 1 cut(s) 99
CfoI GCGC 2 cut(s) 75, 388
Cfr10I RCCGGY 1 cut(s) 293
Cfr13I GGNCC 1 cut(s) 299
CseI GACGC 1 cut(s) 171
CspAI ACCGGT 1 cut(s) 293
CviAII CATG 4 cut(s) 127, 203, 439, 450
CviJI RGCY 9 cut(s) 88, 97, 101, 176, 224, 348, 353, 400, 480
CviKI_1 RGCY 9 cut(s) 88, 97, 101, 176, 224, 348, 353, 400, 480
DdeI CTNAG 2 cut(s) 84, 251
DpnI GATC 5 cut(s) 47, 68, 193, 422, 521
DpnII GATC 5 cut(s) 45, 66, 191, 420, 519
Eco130I CCWWGG 1 cut(s) 126
Eco47I GGWCC 1 cut(s) 299
Eco88I CYCGRG 1 cut(s) 357
EcoNI CCTNNNNNAGG 1 cut(s) 332
EcoT14I CCWWGG 1 cut(s) 126
ErhI CCWWGG 1 cut(s) 126
FaeI CATG 4 cut(s) 130, 206, 442, 453
FaiI YATR 7 cut(s) 128, 166, 204, 404, 433, 440, 451
FatI CATG 4 cut(s) 126, 202, 438, 449
Fnu4HI GCNGC 3 cut(s) 95, 111, 408
FokI GGATG 1 cut(s) 408
Fsp4HI GCNGC 3 cut(s) 95, 111, 408
FspBI CTAG 1 cut(s) 392
FspI TGCGCA 1 cut(s) 74
GlaI GCGC 2 cut(s) 74, 387
GluI GCNGC 3 cut(s) 95, 111, 408
HaeIII GGCC 1 cut(s) 348
HapII CCGG 1 cut(s) 294
HgaI GACGC 1 cut(s) 171
HhaI GCGC 2 cut(s) 75, 388
Hin1II CATG 4 cut(s) 130, 206, 442, 453
Hin6I GCGC 2 cut(s) 73, 386
HinP1I GCGC 2 cut(s) 73, 386
HindIII AAGCTT 1 cut(s) 351
HinfI GANTC 1 cut(s) 488
HpaII CCGG 1 cut(s) 294
Hpy166II GTNNAC 2 cut(s) 53, 299
Hpy188I TCNGA 1 cut(s) 322
Hpy188III TCNNGA 2 cut(s) 64, 357
Hpy8I GTNNAC 2 cut(s) 53, 299
Hpy99I CGWCG 1 cut(s) 187
HpyAV CCTTC 1 cut(s) 328
HpyCH4III ACNGT 2 cut(s) 157, 214
HpyCH4V TGCA 1 cut(s) 428
HpyF10VI GCNNNNNNNGC 3 cut(s) 94, 107, 477
HpyF3I CTNAG 2 cut(s) 84, 251
Hsp92II CATG 4 cut(s) 130, 206, 442, 453
HspAI GCGC 2 cut(s) 73, 386
Kzo9I GATC 5 cut(s) 45, 66, 191, 420, 519
LmnI GCTCC 1 cut(s) 211
LpnPI CCDG 4 cut(s) 77, 83, 307, 330
Lsp1109I GCAGC 1 cut(s) 106
LweI GCATC 1 cut(s) 313
MaeI CTAG 1 cut(s) 392
MaeIII GTNAC 2 cut(s) 151, 159
MalI GATC 5 cut(s) 47, 68, 193, 422, 521
MboI GATC 5 cut(s) 45, 66, 191, 420, 519
MboII GAAGA 1 cut(s) 55
MflI RGATCY 2 cut(s) 66, 191
MluCI AATT 1 cut(s) 473
MmeI TCCRAC 1 cut(s) 233
MnlI CCTC 2 cut(s) 71, 312
MroXI GAANNNNTTC 1 cut(s) 338
MseI TTAA 2 cut(s) 314, 371
MspA1I CMGCKG 1 cut(s) 97
MspI CCGG 1 cut(s) 294
MvnI CGCG 1 cut(s) 524
MwoI GCNNNNNNNGC 3 cut(s) 94, 107, 477
NcoI CCATGG 1 cut(s) 126
NdeII GATC 5 cut(s) 45, 66, 191, 420, 519
NlaIII CATG 4 cut(s) 130, 206, 442, 453
NlaIV GGNNCC 2 cut(s) 264, 411
NsbI TGCGCA 1 cut(s) 74
NspV TTCGAA 1 cut(s) 11
PdmI GAANNNNTTC 1 cut(s) 338
PfeI GAWTC 1 cut(s) 488
PflMI CCANNNNNTGG 1 cut(s) 146
PinAI ACCGGT 1 cut(s) 293
PkrI GCNGC 3 cut(s) 96, 112, 409
PspN4I GGNNCC 2 cut(s) 264, 411
PspPI GGNCC 1 cut(s) 299
PsuI RGATCY 2 cut(s) 66, 191
PvuII CAGCTG 1 cut(s) 97
SaqAI TTAA 2 cut(s) 314, 371
SatI GCNGC 3 cut(s) 95, 111, 408
Sau3AI GATC 5 cut(s) 45, 66, 191, 420, 519
Sau96I GGNCC 1 cut(s) 299
SetI ASST 8 cut(s) 19, 82, 99, 304, 339, 355, 482, 508
SfaNI GCATC 1 cut(s) 313
SfuI TTCGAA 1 cut(s) 11
SinI GGWCC 1 cut(s) 299
Sse9I AATT 1 cut(s) 473
SsiI CCGC 4 cut(s) 110, 407, 469, 495
SspMI CTAG 1 cut(s) 392
StyI CCWWGG 1 cut(s) 126
TaaI ACNGT 2 cut(s) 157, 214
TaqI TCGA 2 cut(s) 11, 36
TasI AATT 1 cut(s) 473
TauI GCSGC 2 cut(s) 113, 410
TfiI GAWTC 1 cut(s) 488
Tru1I TTAA 2 cut(s) 314, 371
Tru9I TTAA 2 cut(s) 314, 371
TscAI CASTG 1 cut(s) 55
TseI GCWGC 1 cut(s) 94
TspDTI ATGAA 1 cut(s) 220
TspRI CASTG 1 cut(s) 55
Van91I CCANNNNNTGG 1 cut(s) 146
VpaK11BI GGWCC 1 cut(s) 299
XagI CCTNNNNNAGG 1 cut(s) 332
XapI RAATTY 1 cut(s) 473
XmnI GAANNNNTTC 1 cut(s) 338
XspI CTAG 1 cut(s) 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.