RchiOBHm_Chr6g0249591

Uncharacterized protein conserved in bacteria (DUF2062)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0007285
Physical Location & Seq
Reverse (-)
5553410 .. 5557151
3742 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22377

Sequence Viewer

Length: 552 bp
ATGGCAAATATTGGGCTGCTCACCATTTGGCTCAACAAGAAGATCGTCGAGCCTCTACTCGAAATCCTTCGCAAGGGTGCCGAGCCTAAGCTGTTGGCCTTCTCTGCAGCTCTTGGCTTCACCATTGGATTGTTTCCAATCTGTGGGACAACTGTGATTATCTGTGGGTTGGCTATTGCATCTCTTGGATCCCACTGCCATGCGCCAACTGTCATGGTGATTAATCTCATTGCTACACCAATCGAACTGAGTTTAATGGTACCGTTCCTGCGCTTTGGTGAATACGTCTCTGGTGGACCTCATTTTCCATTGACTTCTGATGCTTTGAAGAAAGTTTTAACCCTCCAAGCTTCGCATGAAGTCTTATTAAGTATTGCACATGCGTTGTTGGGATGGCTTGTTGCGGCTCCTTTTATTTTTGCCGCAGTGTATATACTCTCTTTACCATTGTTTAAGGTGTTGGTTCACAAGTTTAGCACGCGTCCTTTAAGCCCAAAGAGATCACTTTCACCAACAGAAGGCAGGCTTAAGGATGACGAAAAAGAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2062

Protein Analysis

183

Amino Acids

19.8

Weight (kDa)

9.07

Isoelectric Point (pI)

44.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2062 PF09835 19 - 157 7.5e-07 Uncharacterized protein conserved in bacteria (DUF2062)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 259
AccB1I GGYRCC 2 cut(s) 77, 259
AccB7I CCANNNNNTGG 1 cut(s) 143
AccII CGCG 1 cut(s) 481
AciI CCGC 2 cut(s) 404, 423
AclWI GGATC 2 cut(s) 183, 196
AcsI RAATTY 1 cut(s) 546
AfaI GTAC 1 cut(s) 261
AfiI CCNNNNNNNGG 3 cut(s) 73, 143, 518
AflII CTTAAG 1 cut(s) 527
AflIII ACRYGT 1 cut(s) 479
AgsI TTSAA 1 cut(s) 328
AluBI AGCT 3 cut(s) 91, 110, 350
AluI AGCT 3 cut(s) 91, 110, 350
Alw26I GTCTC 1 cut(s) 292
AlwI GGATC 2 cut(s) 183, 196
AoxI GGCC 1 cut(s) 96
ApeKI GCWGC 2 cut(s) 16, 107
ApoI RAATTY 1 cut(s) 546
AseI ATTAAT 1 cut(s) 222
Asp700I GAANNNNTTC 1 cut(s) 66
Asp718I GGTACC 1 cut(s) 259
AspLEI GCGC 2 cut(s) 205, 273
AspS9I GGNCC 1 cut(s) 296
AsuHPI GGTGA 5 cut(s) 13, 112, 229, 290, 501
AvaII GGWCC 1 cut(s) 296
BamHI GGATCC 1 cut(s) 188
BanI GGYRCC 2 cut(s) 77, 259
BbvI GCAGC 2 cut(s) 3, 119
BccI CCATC 1 cut(s) 387
BcoDI GTCTC 1 cut(s) 292
BfmI CTRYAG 1 cut(s) 105
BfrI CTTAAG 1 cut(s) 527
BisI GCNGC 4 cut(s) 17, 108, 405, 423
BlsI GCNGC 4 cut(s) 18, 109, 406, 424
Bme18I GGWCC 1 cut(s) 296
BmgT120I GGNCC 1 cut(s) 296
BmiI GGNNCC 4 cut(s) 79, 190, 261, 408
BmsI GCATC 2 cut(s) 188, 310
Bpu10I CCTNAGC 1 cut(s) 87
Bsc4I CCNNNNNNNGG 3 cut(s) 73, 143, 518
Bse3DI GCAATG 1 cut(s) 228
BseGI GGATG 2 cut(s) 398, 538
BseLI CCNNNNNNNGG 3 cut(s) 73, 143, 518
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 1 cut(s) 239
BseXI GCAGC 2 cut(s) 3, 119
Bsh1236I CGCG 1 cut(s) 481
BshFI GGCC 1 cut(s) 98
BshNI GGYRCC 2 cut(s) 77, 259
BslFI GGGAC 1 cut(s) 160
BslI CCNNNNNNNGG 3 cut(s) 73, 143, 518
BsmAI GTCTC 1 cut(s) 292
BsmBI CGTCTC 1 cut(s) 292
BsmFI GGGAC 1 cut(s) 160
BsnI GGCC 1 cut(s) 98
Bsp143I GATC 3 cut(s) 42, 188, 500
BspACI CCGC 2 cut(s) 404, 423
BspANI GGCC 1 cut(s) 98
BspCNI CTCAG 1 cut(s) 240
BspFNI CGCG 1 cut(s) 481
BspLI GGNNCC 4 cut(s) 79, 190, 261, 408
BspMAI CTGCAG 1 cut(s) 109
BspPI GGATC 2 cut(s) 183, 196
BspT107I GGYRCC 2 cut(s) 77, 259
BspTI CTTAAG 1 cut(s) 527
BsrDI GCAATG 1 cut(s) 228
BssMI GATC 3 cut(s) 42, 188, 500
Bst4CI ACNGT 3 cut(s) 154, 211, 264
BstAFI CTTAAG 1 cut(s) 527
BstC8I GCNNGC 2 cut(s) 479, 524
BstDEI CTNAG 2 cut(s) 87, 248
BstENI CCTNNNNNAGG 1 cut(s) 71
BstF5I GGATG 2 cut(s) 398, 538
BstFNI CGCG 1 cut(s) 481
BstHHI GCGC 2 cut(s) 205, 273
BstKTI GATC 3 cut(s) 45, 191, 503
BstMAI GTCTC 1 cut(s) 292
BstMBI GATC 3 cut(s) 42, 188, 500
BstMWI GCNNNNNNNGC 1 cut(s) 104
BstNSI RCATGY 1 cut(s) 383
BstSFI CTRYAG 1 cut(s) 105
BstUI CGCG 1 cut(s) 481
BstV1I GCAGC 2 cut(s) 3, 119
BstX2I RGATCY 1 cut(s) 188
BstYI RGATCY 1 cut(s) 188
BsuRI GGCC 1 cut(s) 98
BtsCI GGATG 2 cut(s) 398, 538
BtsI GCAGTG 2 cut(s) 193, 432
BtsIMutI CAGTG 2 cut(s) 193, 432
Cac8I GCNNGC 2 cut(s) 479, 524
CfoI GCGC 2 cut(s) 205, 273
Cfr13I GGNCC 1 cut(s) 296
CseI GACGC 1 cut(s) 470
Csp6I GTAC 1 cut(s) 260
CviAII CATG 4 cut(s) 200, 214, 356, 380
CviQI GTAC 1 cut(s) 260
DdeI CTNAG 2 cut(s) 87, 248
DpnI GATC 3 cut(s) 44, 190, 502
DpnII GATC 3 cut(s) 42, 188, 500
Eco47I GGWCC 1 cut(s) 296
EcoNI CCTNNNNNAGG 1 cut(s) 71
Esp3I CGTCTC 1 cut(s) 292
FaeI CATG 4 cut(s) 203, 217, 359, 383
FaiI YATR 6 cut(s) 201, 215, 357, 381, 432, 434
FalI AAGNNNNNCTT 2 cut(s) 510, 542
FaqI GGGAC 1 cut(s) 160
FatI CATG 4 cut(s) 199, 213, 355, 379
Fnu4HI GCNGC 4 cut(s) 17, 108, 405, 423
FokI GGATG 2 cut(s) 405, 545
Fsp4HI GCNGC 4 cut(s) 17, 108, 405, 423
GlaI GCGC 2 cut(s) 204, 272
GluI GCNGC 4 cut(s) 17, 108, 405, 423
HaeIII GGCC 1 cut(s) 98
HgaI GACGC 1 cut(s) 470
HhaI GCGC 2 cut(s) 205, 273
Hin1II CATG 4 cut(s) 203, 217, 359, 383
Hin6I GCGC 2 cut(s) 203, 271
HinP1I GCGC 2 cut(s) 203, 271
HindIII AAGCTT 1 cut(s) 348
HphI GGTGA 5 cut(s) 13, 112, 229, 290, 501
Hpy166II GTNNAC 2 cut(s) 296, 466
Hpy188I TCNGA 1 cut(s) 319
Hpy8I GTNNAC 2 cut(s) 296, 466
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 3 cut(s) 77, 109, 512
HpyCH4III ACNGT 3 cut(s) 154, 211, 264
HpyCH4IV ACGT 1 cut(s) 285
HpyCH4V TGCA 3 cut(s) 107, 179, 377
HpyF10VI GCNNNNNNNGC 1 cut(s) 104
HpyF3I CTNAG 2 cut(s) 87, 248
HpySE526I ACGT 1 cut(s) 285
Hsp92II CATG 4 cut(s) 203, 217, 359, 383
HspAI GCGC 2 cut(s) 203, 271
KpnI GGTACC 1 cut(s) 263
Kzo9I GATC 3 cut(s) 42, 188, 500
LmnI GCTCC 1 cut(s) 412
LpnPI CCDG 3 cut(s) 276, 281, 508
Lsp1109I GCAGC 2 cut(s) 3, 119
LweI GCATC 2 cut(s) 188, 310
MaeII ACGT 1 cut(s) 285
MalI GATC 3 cut(s) 44, 190, 502
MboI GATC 3 cut(s) 42, 188, 500
MboII GAAGA 2 cut(s) 52, 340
MflI RGATCY 1 cut(s) 188
MluCI AATT 1 cut(s) 546
MluI ACGCGT 1 cut(s) 479
MnlI CCTC 3 cut(s) 63, 309, 353
MroXI GAANNNNTTC 1 cut(s) 66
MseI TTAA 7 cut(s) 222, 254, 338, 368, 453, 488, 528
MslI CAYNNNNRTG 1 cut(s) 198
MspCI CTTAAG 1 cut(s) 527
MvnI CGCG 1 cut(s) 481
MwoI GCNNNNNNNGC 1 cut(s) 104
NdeII GATC 3 cut(s) 42, 188, 500
NlaIII CATG 4 cut(s) 203, 217, 359, 383
NlaIV GGNNCC 4 cut(s) 79, 190, 261, 408
NmeAIII GCCGAG 1 cut(s) 106
NspI RCATGY 1 cut(s) 383
PdmI GAANNNNTTC 1 cut(s) 66
PflMI CCANNNNNTGG 1 cut(s) 143
PkrI GCNGC 4 cut(s) 18, 109, 406, 424
PshBI ATTAAT 1 cut(s) 222
PspN4I GGNNCC 4 cut(s) 79, 190, 261, 408
PspPI GGNCC 1 cut(s) 296
PstI CTGCAG 1 cut(s) 109
PsuI RGATCY 1 cut(s) 188
RsaI GTAC 1 cut(s) 261
RsaNI GTAC 1 cut(s) 260
RseI CAYNNNNRTG 1 cut(s) 198
SaqAI TTAA 7 cut(s) 222, 254, 338, 368, 453, 488, 528
SatI GCNGC 4 cut(s) 17, 108, 405, 423
Sau3AI GATC 3 cut(s) 42, 188, 500
Sau96I GGNCC 1 cut(s) 296
SetI ASST 6 cut(s) 93, 112, 288, 301, 352, 459
SfaNI GCATC 2 cut(s) 188, 310
SfcI CTRYAG 1 cut(s) 105
SinI GGWCC 1 cut(s) 296
SmiMI CAYNNNNRTG 1 cut(s) 198
SmlI CTYRAG 1 cut(s) 527
SmoI CTYRAG 1 cut(s) 527
Sse9I AATT 1 cut(s) 546
SsiI CCGC 2 cut(s) 404, 423
SspI AATATT 1 cut(s) 10
TaaI ACNGT 3 cut(s) 154, 211, 264
TaiI ACGT 1 cut(s) 288
TaqI TCGA 3 cut(s) 48, 60, 243
TasI AATT 1 cut(s) 546
TauI GCSGC 2 cut(s) 407, 425
Tru1I TTAA 7 cut(s) 222, 254, 338, 368, 453, 488, 528
Tru9I TTAA 7 cut(s) 222, 254, 338, 368, 453, 488, 528
TscAI CASTG 2 cut(s) 200, 432
TseI GCWGC 2 cut(s) 16, 107
TspDTI ATGAA 1 cut(s) 372
TspRI CASTG 2 cut(s) 200, 432
Van91I CCANNNNNTGG 1 cut(s) 143
Vha464I CTTAAG 1 cut(s) 527
VpaK11BI GGWCC 1 cut(s) 296
VspI ATTAAT 1 cut(s) 222
XagI CCTNNNNNAGG 1 cut(s) 71
XapI RAATTY 1 cut(s) 546
XceI RCATGY 1 cut(s) 383
XmnI GAANNNNTTC 1 cut(s) 66
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.