transcription, DNA-templated
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G35660 |
| fragaria_vesca | FvH4_2g11773 |
| malus_domestica | MD05G1041500.v1.1 MD10G1047900.v1.1 |
| prunus_persica | Prupe.8G057200_v2.0.a1 Prupe.8G057200_v2.0.a1 Prupe.8G057200_v2.0.a1 |
| pyrus_communis | pycom05g03420 pycom10g03390 |
| rosa_chinensis | RchiOBHm_Chr6g0272031 |
| rosa_laevigata | RLG00000013708 |
| rosa_multiflora | Rmu_sc0023166.1_g000001 |
| rosa_roxburghii | Rroxscaffold_173G00434950 Rroxscaffold_7G00196840 |
| rosa_rugosa | Rorug06G0065000 |
| rosa_samantha | Rh6AG182500 Rh6BG184700 Rh6CG183600 Rh6DG175200 |
| rosa_wichuraiana | Rw6G015700 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 2 cut(s) | 842, 1237 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 1660 |
| Acc36I | ACCTGC | 4 cut(s) | 736, 787, 842, 1237 |
| Acc65I | GGTACC | 1 cut(s) | 231 |
| AccB1I | GGYRCC | 2 cut(s) | 231, 1510 |
| AccI | GTMKAC | 2 cut(s) | 3435, 3949 |
| AccIII | TCCGGA | 1 cut(s) | 4115 |
| AciI | CCGC | 6 cut(s) | 138, 312, 506, 514, 2593, 3498 |
| AclI | AACGTT | 1 cut(s) | 297 |
| AclWI | GGATC | 8 cut(s) | 335, 1298, 1311, 1712, 2105, 2302, 3859, 4107 |
| AcsI | RAATTY | 4 cut(s) | 874, 944, 2366, 4022 |
| AcuI | CTGAAG | 6 cut(s) | 1800, 1917, 2034, 2592, 3162, 3267 |
| AcyI | GRCGYC | 1 cut(s) | 3147 |
| AdeI | CACNNNGTG | 1 cut(s) | 1551 |
| AfaI | GTAC | 7 cut(s) | 233, 604, 1225, 1590, 1945, 2589, 2722 |
| AfeI | AGCGCT | 1 cut(s) | 1333 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 205, 1705, 2458, 4082 |
| AflII | CTTAAG | 2 cut(s) | 458, 3991 |
| AflIII | ACRYGT | 2 cut(s) | 2806, 4103 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 3729 |
| AhlI | ACTAGT | 2 cut(s) | 1928, 1964 |
| AjnI | CCWGG | 3 cut(s) | 541, 1306, 2917 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 3509, 3541 |
| Alw21I | GWGCWC | 3 cut(s) | 2719, 3132, 3241 |
| AlwI | GGATC | 8 cut(s) | 335, 1298, 1311, 1712, 2105, 2302, 3859, 4107 |
| Ama87I | CYCGRG | 5 cut(s) | 492, 709, 1124, 3116, 3752 |
| Aor13HI | TCCGGA | 1 cut(s) | 4115 |
| Aor51HI | AGCGCT | 1 cut(s) | 1333 |
| AoxI | GGCC | 5 cut(s) | 1261, 2411, 3958, 4029, 4062 |
| ApeKI | GCWGC | 9 cut(s) | 14, 2655, 2700, 2993, 3365, 3500, 3503, 3662, 3705 |
| ApoI | RAATTY | 4 cut(s) | 874, 944, 2366, 4022 |
| AseI | ATTAAT | 1 cut(s) | 1622 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 874, 1447, 3255 |
| Asp718I | GGTACC | 1 cut(s) | 231 |
| AspLEI | GCGC | 4 cut(s) | 1334, 1412, 1535, 1573 |
| AspS9I | GGNCC | 6 cut(s) | 545, 1696, 2411, 3833, 4003, 4030 |
| AsuC2I | CCSGG | 1 cut(s) | 334 |
| AsuHPI | GGTGA | 8 cut(s) | 115, 322, 1349, 1410, 2120, 2213, 2873, 4203 |
| AvaI | CYCGRG | 5 cut(s) | 492, 709, 1124, 3116, 3752 |
| AvaII | GGWCC | 4 cut(s) | 545, 1696, 3833, 4003 |
| BaeGI | GKGCMC | 1 cut(s) | 1125 |
| BamHI | GGATCC | 1 cut(s) | 1303 |
| BanI | GGYRCC | 2 cut(s) | 231, 1510 |
| BanII | GRGCYC | 2 cut(s) | 3132, 3241 |
| BauI | CACGAG | 4 cut(s) | 1604, 2635, 2787, 3507 |
| BbsI | GAAGAC | 4 cut(s) | 894, 1773, 2856, 3150 |
| Bbv12I | GWGCWC | 3 cut(s) | 2719, 3132, 3241 |
| BbvI | GCAGC | 8 cut(s) | 2667, 2712, 2980, 3377, 3487, 3515, 3649, 3692 |
| BceAI | ACGGC | 7 cut(s) | 22, 299, 1168, 1276, 2051, 2820, 3945 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 4166, 4200 |
| BciT130I | CCWGG | 3 cut(s) | 543, 1308, 2919 |
| BclI | TGATCA | 1 cut(s) | 1576 |
| BcnI | CCSGG | 1 cut(s) | 334 |
| BcuI | ACTAGT | 2 cut(s) | 1928, 1964 |
| BfmI | CTRYAG | 7 cut(s) | 780, 1905, 2357, 3049, 3162, 3393, 3501 |
| BfoI | RGCGCY | 1 cut(s) | 1335 |
| BfrI | CTTAAG | 2 cut(s) | 458, 3991 |
| BfuAI | ACCTGC | 4 cut(s) | 736, 787, 842, 1237 |
| BglII | AGATCT | 1 cut(s) | 256 |
| Bme1390I | CCNGG | 4 cut(s) | 334, 543, 1308, 2919 |
| Bme18I | GGWCC | 4 cut(s) | 545, 1696, 3833, 4003 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 3729 |
| BmeT110I | CYCGRG | 5 cut(s) | 492, 709, 1124, 3116, 3752 |
| BmgT120I | GGNCC | 6 cut(s) | 545, 1696, 2411, 3833, 4003, 4030 |
| BmiI | GGNNCC | 9 cut(s) | 142, 233, 1305, 1512, 1541, 2183, 3110, 3258, 4005 |
| BmrFI | CCNGG | 4 cut(s) | 334, 543, 1308, 2919 |
| BmsI | GCATC | 8 cut(s) | 24, 694, 1164, 1558, 2053, 2962, 3148, 3292 |
| BoxI | GACNNNNGTC | 1 cut(s) | 530 |
| BpiI | GAAGAC | 4 cut(s) | 894, 1773, 2856, 3150 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 692, 724, 3868, 3900 |
| BpmI | CTGGAG | 8 cut(s) | 741, 753, 927, 1329, 1900, 2901, 2906, 3740 |
| BpuEI | CTTGAG | 5 cut(s) | 1375, 1992, 2936, 3592, 4142 |
| BpuMI | CCSGG | 1 cut(s) | 334 |
| Bsa29I | ATCGAT | 1 cut(s) | 3818 |
| BsaBI | GATNNNNATC | 1 cut(s) | 261 |
| BsaHI | GRCGYC | 1 cut(s) | 3147 |
| BsaI | GGTCTC | 2 cut(s) | 1742, 2788 |
| BsaJI | CCNNGG | 5 cut(s) | 227, 333, 541, 1162, 3999 |
| BsaWI | WCCGGW | 1 cut(s) | 4115 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2309, 2339 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 205, 1705, 2458, 4082 |
| Bse118I | RCCGGY | 1 cut(s) | 862 |
| Bse1I | ACTGG | 3 cut(s) | 122, 1917, 3597 |
| Bse3DI | GCAATG | 3 cut(s) | 1024, 2710, 4194 |
| Bse8I | GATNNNNATC | 1 cut(s) | 261 |
| BseAI | TCCGGA | 1 cut(s) | 4115 |
| BseBI | CCWGG | 3 cut(s) | 543, 1308, 2919 |
| BseCI | ATCGAT | 1 cut(s) | 3818 |
| BseDI | CCNNGG | 5 cut(s) | 227, 333, 541, 1162, 3999 |
| BseGI | GGATG | 9 cut(s) | 622, 663, 814, 1060, 1225, 1237, 1573, 2068, 2478 |
| BseJI | GATNNNNATC | 1 cut(s) | 261 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 205, 1705, 2458, 4082 |
| BseMI | GCAATG | 3 cut(s) | 1024, 2710, 4194 |
| BseNI | ACTGG | 3 cut(s) | 122, 1917, 3597 |
| BseRI | GAGGAG | 3 cut(s) | 185, 1718, 2330 |
| BseSI | GKGCMC | 1 cut(s) | 1125 |
| BseXI | GCAGC | 8 cut(s) | 2667, 2712, 2980, 3377, 3487, 3515, 3649, 3692 |
| BshFI | GGCC | 5 cut(s) | 1263, 2413, 3960, 4031, 4064 |
| BshNI | GGYRCC | 2 cut(s) | 231, 1510 |
| BshVI | ATCGAT | 1 cut(s) | 3818 |
| BsiHKAI | GWGCWC | 3 cut(s) | 2719, 3132, 3241 |
| BsiHKCI | CYCGRG | 5 cut(s) | 492, 709, 1124, 3116, 3752 |
| BsiSI | CCGG | 3 cut(s) | 333, 863, 4116 |
| BslFI | GGGAC | 3 cut(s) | 95, 2557, 3334 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 205, 1705, 2458, 4082 |
| BsmBI | CGTCTC | 1 cut(s) | 3119 |
| BsmFI | GGGAC | 3 cut(s) | 95, 2557, 3334 |
| BsmI | GAATGC | 6 cut(s) | 646, 3170, 3477, 3665, 4062, 4189 |
| BsnI | GGCC | 5 cut(s) | 1263, 2413, 3960, 4031, 4064 |
| Bso31I | GGTCTC | 2 cut(s) | 1742, 2788 |
| BsoBI | CYCGRG | 5 cut(s) | 492, 709, 1124, 3116, 3752 |
| Bsp1286I | GDGCHC | 4 cut(s) | 1125, 2719, 3132, 3241 |
| Bsp13I | TCCGGA | 1 cut(s) | 4115 |
| Bsp1407I | TGTACA | 1 cut(s) | 602 |
| BspACI | CCGC | 6 cut(s) | 138, 312, 506, 514, 2593, 3498 |
| BspANI | GGCC | 5 cut(s) | 1263, 2413, 3960, 4031, 4064 |
| BspDI | ATCGAT | 1 cut(s) | 3818 |
| BspEI | TCCGGA | 1 cut(s) | 4115 |
| BspHI | TCATGA | 5 cut(s) | 745, 1144, 2071, 2371, 3538 |
| BspLI | GGNNCC | 9 cut(s) | 142, 233, 1305, 1512, 1541, 2183, 3110, 3258, 4005 |
| BspMAI | CTGCAG | 5 cut(s) | 784, 1909, 2361, 3397, 3505 |
| BspMI | ACCTGC | 4 cut(s) | 736, 787, 842, 1237 |
| BspPI | GGATC | 8 cut(s) | 335, 1298, 1311, 1712, 2105, 2302, 3859, 4107 |
| BspQI | GCTCTTC | 2 cut(s) | 827, 3016 |
| BspT107I | GGYRCC | 2 cut(s) | 231, 1510 |
| BspTI | CTTAAG | 2 cut(s) | 458, 3991 |
| BspTNI | GGTCTC | 2 cut(s) | 1742, 2788 |
| BsrDI | GCAATG | 3 cut(s) | 1024, 2710, 4194 |
| BsrFI | RCCGGY | 1 cut(s) | 862 |
| BsrGI | TGTACA | 1 cut(s) | 602 |
| BsrI | ACTGG | 3 cut(s) | 122, 1917, 3597 |
| BssAI | RCCGGY | 1 cut(s) | 862 |
| BssECI | CCNNGG | 5 cut(s) | 227, 333, 541, 1162, 3999 |
| BssNI | GRCGYC | 1 cut(s) | 3147 |
| BssSI | CACGAG | 4 cut(s) | 1604, 2635, 2787, 3507 |
| BssT1I | CCWWGG | 1 cut(s) | 1162 |
| Bst2BI | CACGAG | 4 cut(s) | 1604, 2635, 2787, 3507 |
| Bst2UI | CCWGG | 3 cut(s) | 543, 1308, 2919 |
| Bst6I | CTCTTC | 4 cut(s) | 552, 827, 2459, 3016 |
| BstACI | GRCGYC | 1 cut(s) | 3147 |
| BstAFI | CTTAAG | 2 cut(s) | 458, 3991 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 4193 |
| BstAUI | TGTACA | 1 cut(s) | 602 |
| BstC8I | GCNNGC | 8 cut(s) | 1043, 1414, 2281, 2677, 2776, 3447, 4062, 4194 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1703 |
| BstF5I | GGATG | 9 cut(s) | 622, 663, 814, 1060, 1225, 1237, 1573, 2068, 2478 |
| BstH2I | RGCGCY | 1 cut(s) | 1335 |
| BstHHI | GCGC | 4 cut(s) | 1334, 1412, 1535, 1573 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 728, 2685, 3146, 3167, 3602, 3668, 3794, 4193 |
| BstNI | CCWGG | 3 cut(s) | 543, 1308, 2919 |
| BstNSI | RCATGY | 2 cut(s) | 4107, 4196 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 530 |
| BstSCI | CCNGG | 4 cut(s) | 332, 541, 1306, 2917 |
| BstSFI | CTRYAG | 7 cut(s) | 780, 1905, 2357, 3049, 3162, 3393, 3501 |
| BstSLI | GKGCMC | 1 cut(s) | 1125 |
| BstV1I | GCAGC | 8 cut(s) | 2667, 2712, 2980, 3377, 3487, 3515, 3649, 3692 |
| BstV2I | GAAGAC | 4 cut(s) | 894, 1773, 2856, 3150 |
| BstX2I | RGATCY | 3 cut(s) | 256, 1303, 4112 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 4027 |
| BstYI | RGATCY | 3 cut(s) | 256, 1303, 4112 |
| Bsu15I | ATCGAT | 1 cut(s) | 3818 |
| BsuRI | GGCC | 5 cut(s) | 1263, 2413, 3960, 4031, 4064 |
| BsuTUI | ATCGAT | 1 cut(s) | 3818 |
| BtsCI | GGATG | 9 cut(s) | 622, 663, 814, 1060, 1225, 1237, 1573, 2068, 2478 |
| BtsI | GCAGTG | 6 cut(s) | 219, 1914, 2354, 2686, 2695, 3390 |
| BveI | ACCTGC | 4 cut(s) | 736, 787, 842, 1237 |
| Cac8I | GCNNGC | 8 cut(s) | 1043, 1414, 2281, 2677, 2776, 3447, 4062, 4194 |
| CciI | TCATGA | 5 cut(s) | 745, 1144, 2071, 2371, 3538 |
| CfoI | GCGC | 4 cut(s) | 1334, 1412, 1535, 1573 |
| Cfr10I | RCCGGY | 1 cut(s) | 862 |
| Cfr13I | GGNCC | 6 cut(s) | 545, 1696, 2411, 3833, 4003, 4030 |
| ClaI | ATCGAT | 1 cut(s) | 3818 |
| CseI | GACGC | 4 cut(s) | 691, 3155, 3611, 3719 |
| Csp6I | GTAC | 7 cut(s) | 232, 603, 1224, 1589, 1944, 2588, 2721 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 594, 629 |
| CviQI | GTAC | 7 cut(s) | 232, 603, 1224, 1589, 1944, 2588, 2721 |
| DraI | TTTAAA | 4 cut(s) | 633, 1557, 2559, 2818 |
| DraIII | CACNNNGTG | 1 cut(s) | 1551 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1660 |
| DriI | GACNNNNNGTC | 1 cut(s) | 3729 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1660 |
| Eam1104I | CTCTTC | 4 cut(s) | 552, 827, 2459, 3016 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 3729 |
| EarI | CTCTTC | 4 cut(s) | 552, 827, 2459, 3016 |
| Ecl136II | GAGCTC | 2 cut(s) | 3130, 3239 |
| Eco130I | CCWWGG | 1 cut(s) | 1162 |
| Eco24I | GRGCYC | 2 cut(s) | 3132, 3241 |
| Eco31I | GGTCTC | 2 cut(s) | 1742, 2788 |
| Eco32I | GATATC | 2 cut(s) | 2077, 2137 |
| Eco47I | GGWCC | 4 cut(s) | 545, 1696, 3833, 4003 |
| Eco47III | AGCGCT | 1 cut(s) | 1333 |
| Eco53kI | GAGCTC | 2 cut(s) | 3130, 3239 |
| Eco57I | CTGAAG | 6 cut(s) | 1800, 1917, 2034, 2592, 3162, 3267 |
| Eco88I | CYCGRG | 5 cut(s) | 492, 709, 1124, 3116, 3752 |
| EcoICRI | GAGCTC | 2 cut(s) | 3130, 3239 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1703 |
| EcoO109I | RGGNCCY | 1 cut(s) | 4003 |
| EcoRI | GAATTC | 1 cut(s) | 874 |
| EcoRII | CCWGG | 3 cut(s) | 541, 1306, 2917 |
| EcoRV | GATATC | 2 cut(s) | 2077, 2137 |
| EcoT14I | CCWWGG | 1 cut(s) | 1162 |
| EcoT22I | ATGCAT | 3 cut(s) | 648, 2068, 4194 |
| EcoT38I | GRGCYC | 2 cut(s) | 3132, 3241 |
| ErhI | CCWWGG | 1 cut(s) | 1162 |
| Esp3I | CGTCTC | 1 cut(s) | 3119 |
| FaqI | GGGAC | 3 cut(s) | 95, 2557, 3334 |
| FauI | CCCGC | 1 cut(s) | 507 |
| FauNDI | CATATG | 2 cut(s) | 1597, 3979 |
| FbaI | TGATCA | 1 cut(s) | 1576 |
| FblI | GTMKAC | 2 cut(s) | 3435, 3949 |
| FokI | GGATG | 9 cut(s) | 629, 670, 821, 1067, 1224, 1232, 1580, 2075, 2485 |
| FriOI | GRGCYC | 2 cut(s) | 3132, 3241 |
| GlaI | GCGC | 4 cut(s) | 1333, 1411, 1534, 1572 |
| GsuI | CTGGAG | 8 cut(s) | 741, 753, 927, 1329, 1900, 2901, 2906, 3740 |
| HaeII | RGCGCY | 1 cut(s) | 1335 |
| HaeIII | GGCC | 5 cut(s) | 1263, 2413, 3960, 4031, 4064 |
| HapII | CCGG | 3 cut(s) | 333, 863, 4116 |
| HgaI | GACGC | 4 cut(s) | 691, 3155, 3611, 3719 |
| HhaI | GCGC | 4 cut(s) | 1334, 1412, 1535, 1573 |
| Hin1I | GRCGYC | 1 cut(s) | 3147 |
| Hin6I | GCGC | 4 cut(s) | 1332, 1410, 1533, 1571 |
| HinP1I | GCGC | 4 cut(s) | 1332, 1410, 1533, 1571 |
| HincII | GTYRAC | 2 cut(s) | 2610, 4164 |
| HindII | GTYRAC | 2 cut(s) | 2610, 4164 |
| HindIII | AAGCTT | 3 cut(s) | 66, 1092, 2226 |
| HpaI | GTTAAC | 1 cut(s) | 2610 |
| HpaII | CCGG | 3 cut(s) | 333, 863, 4116 |
| HphI | GGTGA | 8 cut(s) | 115, 322, 1349, 1410, 2120, 2213, 2873, 4203 |
| Hpy99I | CGWCG | 4 cut(s) | 34, 218, 685, 3237 |
| HpyAV | CCTTC | 6 cut(s) | 210, 1892, 3686, 3858, 3950, 4076 |
| HpyCH4IV | ACGT | 4 cut(s) | 297, 1678, 2806, 3580 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 728, 2685, 3146, 3167, 3602, 3668, 3794, 4193 |
| HpySE526I | ACGT | 4 cut(s) | 297, 1678, 2806, 3580 |
| Hsp92I | GRCGYC | 1 cut(s) | 3147 |
| HspAI | GCGC | 4 cut(s) | 1332, 1410, 1533, 1571 |
| Kpn2I | TCCGGA | 1 cut(s) | 4115 |
| KpnI | GGTACC | 1 cut(s) | 235 |
| Ksp22I | TGATCA | 1 cut(s) | 1576 |
| KspAI | GTTAAC | 1 cut(s) | 2610 |
| LguI | GCTCTTC | 2 cut(s) | 827, 3016 |
| Lsp1109I | GCAGC | 8 cut(s) | 2667, 2712, 2980, 3377, 3487, 3515, 3649, 3692 |
| LweI | GCATC | 8 cut(s) | 24, 694, 1164, 1558, 2053, 2962, 3148, 3292 |
| MaeII | ACGT | 4 cut(s) | 297, 1678, 2806, 3580 |
| MfeI | CAATTG | 1 cut(s) | 4034 |
| MflI | RGATCY | 3 cut(s) | 256, 1303, 4112 |
| MhlI | GDGCHC | 4 cut(s) | 1125, 2719, 3132, 3241 |
| MlyI | GAGTC | 3 cut(s) | 520, 1670, 4115 |
| MmeI | TCCRAC | 5 cut(s) | 53, 393, 499, 1812, 3086 |
| Mph1103I | ATGCAT | 3 cut(s) | 648, 2068, 4194 |
| MroI | TCCGGA | 1 cut(s) | 4115 |
| MroXI | GAANNNNTTC | 3 cut(s) | 874, 1447, 3255 |
| MslI | CAYNNNNRTG | 4 cut(s) | 750, 1242, 1743, 3195 |
| MspA1I | CMGCKG | 4 cut(s) | 514, 2593, 2658, 3500 |
| MspCI | CTTAAG | 2 cut(s) | 458, 3991 |
| MspI | CCGG | 3 cut(s) | 333, 863, 4116 |
| MspR9I | CCNGG | 4 cut(s) | 334, 543, 1308, 2919 |
| MunI | CAATTG | 1 cut(s) | 4034 |
| Mva1269I | GAATGC | 6 cut(s) | 646, 3170, 3477, 3665, 4062, 4189 |
| MvaI | CCWGG | 3 cut(s) | 543, 1308, 2919 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 728, 2685, 3146, 3167, 3602, 3668, 3794, 4193 |
| NciI | CCSGG | 1 cut(s) | 334 |
| NdeI | CATATG | 2 cut(s) | 1597, 3979 |
| NlaIV | GGNNCC | 9 cut(s) | 142, 233, 1305, 1512, 1541, 2183, 3110, 3258, 4005 |
| NmuCI | GTSAC | 9 cut(s) | 217, 1634, 1753, 1845, 2666, 3224, 3345, 3599, 3962 |
| NsiI | ATGCAT | 3 cut(s) | 648, 2068, 4194 |
| NspI | RCATGY | 2 cut(s) | 4107, 4196 |
| PaeI | GCATGC | 1 cut(s) | 4196 |
| PaeR7I | CTCGAG | 1 cut(s) | 709 |
| PagI | TCATGA | 5 cut(s) | 745, 1144, 2071, 2371, 3538 |
| PaqCI | CACCTGC | 2 cut(s) | 842, 1237 |
| PciI | ACATGT | 1 cut(s) | 4103 |
| PciSI | GCTCTTC | 2 cut(s) | 827, 3016 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 3273 |
| PctI | GAATGC | 6 cut(s) | 646, 3170, 3477, 3665, 4062, 4189 |
| PdmI | GAANNNNTTC | 3 cut(s) | 874, 1447, 3255 |
| PfoI | TCCNGGA | 2 cut(s) | 1306, 2917 |
| PleI | GAGTC | 3 cut(s) | 520, 1669, 4115 |
| PpsI | GAGTC | 3 cut(s) | 520, 1669, 4115 |
| PpuMI | RGGWCCY | 1 cut(s) | 4003 |
| PscI | ACATGT | 1 cut(s) | 4103 |
| PshAI | GACNNNNGTC | 1 cut(s) | 530 |
| PshBI | ATTAAT | 1 cut(s) | 1622 |
| Psp124BI | GAGCTC | 2 cut(s) | 3132, 3241 |
| Psp1406I | AACGTT | 1 cut(s) | 297 |
| Psp5II | RGGWCCY | 1 cut(s) | 4003 |
| Psp6I | CCWGG | 3 cut(s) | 541, 1306, 2917 |
| PspGI | CCWGG | 3 cut(s) | 541, 1306, 2917 |
| PspN4I | GGNNCC | 9 cut(s) | 142, 233, 1305, 1512, 1541, 2183, 3110, 3258, 4005 |
| PspPI | GGNCC | 6 cut(s) | 545, 1696, 2411, 3833, 4003, 4030 |
| PspPPI | RGGWCCY | 1 cut(s) | 4003 |
| PspXI | VCTCGAGB | 1 cut(s) | 709 |
| PstI | CTGCAG | 5 cut(s) | 784, 1909, 2361, 3397, 3505 |
| PsuI | RGATCY | 3 cut(s) | 256, 1303, 4112 |
| PvuII | CAGCTG | 1 cut(s) | 2658 |
| RsaI | GTAC | 7 cut(s) | 233, 604, 1225, 1590, 1945, 2589, 2722 |
| RsaNI | GTAC | 7 cut(s) | 232, 603, 1224, 1589, 1944, 2588, 2721 |
| RseI | CAYNNNNRTG | 4 cut(s) | 750, 1242, 1743, 3195 |
| SacI | GAGCTC | 2 cut(s) | 3132, 3241 |
| SapI | GCTCTTC | 2 cut(s) | 827, 3016 |
| Sau96I | GGNCC | 6 cut(s) | 545, 1696, 2411, 3833, 4003, 4030 |
| SbfI | CCTGCAGG | 1 cut(s) | 784 |
| SchI | GAGTC | 3 cut(s) | 520, 1670, 4115 |
| ScrFI | CCNGG | 4 cut(s) | 334, 543, 1308, 2919 |
| SdaI | CCTGCAGG | 1 cut(s) | 784 |
| SduI | GDGCHC | 4 cut(s) | 1125, 2719, 3132, 3241 |
| SfaNI | GCATC | 8 cut(s) | 24, 694, 1164, 1558, 2053, 2962, 3148, 3292 |
| SfcI | CTRYAG | 7 cut(s) | 780, 1905, 2357, 3049, 3162, 3393, 3501 |
| Sfr274I | CTCGAG | 1 cut(s) | 709 |
| SinI | GGWCC | 4 cut(s) | 545, 1696, 3833, 4003 |
| SlaI | CTCGAG | 1 cut(s) | 709 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 750, 1242, 1743, 3195 |
| SmlI | CTYRAG | 8 cut(s) | 458, 709, 1390, 2007, 2951, 3571, 3991, 4157 |
| SmoI | CTYRAG | 8 cut(s) | 458, 709, 1390, 2007, 2951, 3571, 3991, 4157 |
| SpeI | ACTAGT | 2 cut(s) | 1928, 1964 |
| SphI | GCATGC | 1 cut(s) | 4196 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 784 |
| SsiI | CCGC | 6 cut(s) | 138, 312, 506, 514, 2593, 3498 |
| SspI | AATATT | 2 cut(s) | 1801, 2940 |
| SstI | GAGCTC | 2 cut(s) | 3132, 3241 |
| StyD4I | CCNGG | 4 cut(s) | 332, 541, 1306, 2917 |
| StyI | CCWWGG | 1 cut(s) | 1162 |
| TaiI | ACGT | 4 cut(s) | 300, 1681, 2809, 3583 |
| TaqII | GACCGA | 1 cut(s) | 1475 |
| TatI | WGTACW | 2 cut(s) | 602, 2587 |
| TauI | GCSGC | 3 cut(s) | 141, 314, 2596 |
| TseFI | GTSAC | 9 cut(s) | 217, 1634, 1753, 1845, 2666, 3224, 3345, 3599, 3962 |
| TseI | GCWGC | 9 cut(s) | 14, 2655, 2700, 2993, 3365, 3500, 3503, 3662, 3705 |
| Tsp45I | GTSAC | 9 cut(s) | 217, 1634, 1753, 1845, 2666, 3224, 3345, 3599, 3962 |
| TspGWI | ACGGA | 5 cut(s) | 49, 435, 1709, 2129, 2870 |
| Vha464I | CTTAAG | 2 cut(s) | 458, 3991 |
| VpaK11BI | GGWCC | 4 cut(s) | 545, 1696, 3833, 4003 |
| VspI | ATTAAT | 1 cut(s) | 1622 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1703 |
| XapI | RAATTY | 4 cut(s) | 874, 944, 2366, 4022 |
| XceI | RCATGY | 2 cut(s) | 4107, 4196 |
| XhoI | CTCGAG | 1 cut(s) | 709 |
| XmiI | GTMKAC | 2 cut(s) | 3435, 3949 |
| XmnI | GAANNNNTTC | 3 cut(s) | 874, 1447, 3255 |
| Zsp2I | ATGCAT | 3 cut(s) | 648, 2068, 4194 |