MD10G1047900.v1.1

transcription, DNA-templated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011317
Physical Location & Seq
Forward (+)
6383066 .. 6389718
6653 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1047900.v1.1.491

Sequence Viewer

Length: 4374 bp
ATGGAGAAATCATGGTCATCGTCGGCACCGTTGAGCTCCGATGGCTCCCGAGAGCTTCAGTGCATCGGCAAGTTAGAAATTGAAAGGCCGAACCCCGTCGGCTTCCTCTGCGGCTCCATCCCTGTTCCCACCGACAAAGCCTTCCACTCTTGCGATTCCGCTCTCATTCCCTCCCGCCAAACAGTGAGTGCTCCGCGGTATCGAATGCTTCCGACGGAGACGGACCTGAAGAGCCCGCCGTCGCCGGACAATGTTCTTCCCATTGCTGCTGTGCACCCCAAGGCCGCCGGAGATATAGCTTGGGATAGTGGTACTGTTTCATCAAATCTCACTAGAAAATGTGAGGCCCTTGCGGTGTCTGGATTGGTTGATTATGGGGATGAGATCGACGTCATTGCTCCAGCTGACATTCTGAAGCAGATCTTCAAAATGCCGTATTCCAAGGCTCGATTATCAATTGCAGTTCAGCGTGTTGGACAGACGCTTGTTCTCAATACTGGGCCTGACATCGAAGAGGGGGAAAAGTTAATAAGGAGACGTAAAAATCAATCAAAATGTGGAGATCAGTCTTTATTTTTGAATTTTGCTATGCATTCTGTTCGGATGGAAGCTTGTGATTGCCCGCCAACTCATCATGTTCCATCACAAGAGCAATCAAAATCCTCTGTTCTTCCTGGGGCAAATACTCAGTTTGTGGGACAGCATGATGATGTTGCTCCAGGTGGAAAATCAAATAGTCCAGAGTACACACAGGTTAAAAGGGATGATTTCTTTTTGGCAAATAAGAAGGATAAGAAAAATAAGGGGCGCAATCCTGTCAAAAAGACTTCACAAGTTGGCGAGAAGTCCAGGAGCTCAATACAAGAGTCTGAAAAACATAGAAGAGTTGGTAACGACGGGTTTCTAAGGGTTTTGTTTTGGCAATTTCACAATTTCCGCATGCTCATAGGGAGTGACTTGCTTCTGTTTAGTAATGAAAAATATGTTGCTGTTAGCTTGCATCTCTGGGATGTGTCACGACAGGTTACACCATTAACTTGGCTTGAGGCCTGGCTTGACAATGTCATGGCAAGTGTGCCTGAGATGGCCATTTGTTATCATGAAAACGGTGTTGTGCAGGGATACGAGCTTTTGAAAACAGATGATATATTTCTACTAAAAGGGATCTCTGAGGATGGCACTCCTGCTTTTCATCCTCATGTGGTGCAACAAAACGGCCTTTCTGTTTTGAGGTTCCTTCAAGAAAACTGCAAGCAAGATCCTGGAGCTTATTGGCTGTATAAAAATGCTGGGGAAGATGTCATTCAACTCTTCGATCTTTCTGTGATTCCCAAGAACTGTTCTTCTGATGATTGTGATGATAGCTCAAGCTCCCTTCCTTCTCTATTGCATCGAGGAAGAAGTGATTCTCTGTATTCATTGGGGACTCTCTTGTATCGAATTGCTCACAGGCTTTCACTTTCAGTGGCTCCAAATAATATGGCAAGGTGTGCTAGGTTTTTCAAAAAATGTTTGGAATTCCTAGATGCGCCAGATCATCTGGTTGTGCGGGCATCTGCACATGAACAATTTGCAAGGCTTATTTTGAACCACGATGAAGAGCTGGAATTAGAGCCTGATGATCTGCCTGTAGAATGTGAACTTACAGTCACTGATGCTGAAGAAGATTCTTTGGACTTTTTAAGTAGCAATTCTGAATCGATGGTACATGAAGAACCTCAACTGGTGGGACAGGAAAAATCATATGACGATGATCAAAATGTTGAAGATTTAGTAACTGAAGCTTCTGTAAAGATGACTTTAGAAGCAAATGCATACTCTCCCAGAAATTTGATAGCAGAAGGTAGCACAGACTACGGAGATTTGACAGAAGCTGCACCAAATTCCTCTGGTATTGAAAGCTCTGCAGTTTGCAAATTGCCTGCAACAACCGGACATGTAGTTCAAACTGTTGCTGAACCAATCTCTTCTAAGCTAGCTGCAATACATCATGTTTCTCAAGCTATTAAATCTCTCAGATGGATGCGCCAACTGCAAACTACTGAATCAAAGTTGATGGACCAGGAGAGTGAAACCCATGATAGGCCACCTTCATCTGTTAATCTTTCTGTTTGTGCATGTGGTGATGCTGACTGTATCGAAGTTTGTGACATTAGAGAATGGCTTCCAACATCAACATTGGATCACAAGTTGTGGAAACTTGTTCTTTTGCTTGGAGAGTCATATTTGGCACTTGGACAAGCTTACAAGGAGGATGGTCAGTTGCATCAAGCTTTGAAGGTTGTAGAATTGGCCTGTTCTGTATATGGATCAATGCCTCAACATCTTGAAGACACAAAATTCATTTCTTCCATGAATAGTTGTTTTCCATCACCAACAAAATTCAGTTATACAAATAAAAAGATTACATCATCTAATAGTGATCGGGAAGATCTGAGCTCCAGCTCCACTCATGGTTGTCTTTCTTTCGAGCAGTTCTCATCCATCTACCTCTTCTGGGCCAAGGCATGGACACTGGTTGGAGATGTTTATGTTGAATTCCATTTGGCTAAGGATAGTATCACCTCTCCACCAGCAGAGGGAAAATATTCTATTAGAGAGTTGAAAGTTTCATCCGAGGTTGTCAAAGAAGTTAAAAGGCTAAAGAAGAAGTTGGGGCACAATACACAAAACTGCAGTTCATGTTCTCTAGTAAATTGCAGCTGCCAGAGTGATAGGGCAAGCAGTGGTAGCAGTGCAAGTAGTAGTAGTGGCAATATGCGCTTGGTAAATTCTGGCAGGAAATACAGTAAAAGACCATATGCTAAGAGCAATGCCTTCTCGCTTGCAAGAGAGACTGAAGATGATAATCCTTGTTTGAAAACTGAGAGTGGCAAGGTGTCTGATTGTGGATATCTACACCAAAAGAGAAACGGTGAAACCATAGAACAATCTTCAAATATGGATAAGCTTACAGTGAAACATGTGTCATCTGATAATTCTGACAATTTAGAGGGCACTTTAGGAGTGCATGACATGGGACCGATACTGGCCTCTCAATCTAATGCTTCTTTGAGAGAAACAACGAAAGTGAAAAATGGTGGGATATTCAAGTTCCTTGGAGGTCCTGCAGTTGGAGATGCTGAAAACAATCTTTCGGAAGCCTGTTGCTGTTACGAAGCTGCTACAAAGGCATTAGGTGGTCTTCCTTCCGGTTCTGCTGAACTACAATCTATAATGAAAAAGAAAGGGTGGGTATGCAATGAACTGGGTCGAAACAGACTTGAAAGGAAAGAACTAAAAAAAGCTGAATTTGCTTTTGTAGATGCTATAAAAGCATTCAGAGAAGTCTCTGATCATACCAACATCATATTGATAAACTGTAACTTGGGGCATGGAAGACGGGCATTGGCAGAAGAGATGGTATCAAAGATTGACGGTCTCAAGGGGCATCCGATCTTTCATAACGCATACAACCATGCATTGGAGACCACAAAGTTAGAATATAGTGAATCCCTTAAGTATTATGGGGCAGCAAAAGCTGAACTCAATGCTTTTGTTGCAGAGGCTGGCTCTGAACTAAACAATTTGAGGACTGAGGTATATACACAGTTTGCTCATACGTATCTGAGGCTCGGAATGTTCTTGGCAAGAGAAGATACAACTGTTGATGTCTATGAAACTGGAGTCGTGCATGTCAGTTCCACTAGTCCTAGTGGCAGAAAATCAAGAAAACAATTTCGTAAGCATGAGATTTCAGCCAACGATGCCATTAGAGAGGCACTGTCTGTTTATGAATCATTAGGTGAATTGCGGAAACAGGAGGCTGCATATTCCTATTTTCAGTTGGCTTGCTACCAAAGGGATTGTTGTATGAAATTCTTAGAGCTAGATCATAAGAAAAACAACTTACCCAAAGGCGAAAACACAATTCTCCAACGAGTCAAGCAGTATGCATCCTTGGCAGAGAGGAACATGCAAAAGGCCATGGATTTCTACAGCCCAAAAACGCATCCTACCATGTACCTAACTATTCTTATAGAGAGATCAGCTCTTTCATTGAGCCTTTCAAGCCCCTTACACTCAAATGCAATGTTGGAATCAGCTCTGTCTTGCATGCTTGAAGGACGGTATGTGTCAGAAACAGACTCGGACTCTTGGAAAACCGATCACCCCGAGGTACACGCCAAGTTTTGGAATCAGTTGCAGATGATTTTGAAGAAGATGCTGGGTGTGGCACTTTCTTCACGCACAAGCAAATCTTCTGTTTCCCAGCCAGCTTCAGTATCCAGCAGGTGTGGGGATACTGAAAAATTGAGGGGGCTTTACAAACTCTCTCTCAAATCCACAAACTTAAATCAATTGGATGCTATTCACTCCTTGTGGACATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1458

Amino Acids

161.53

Weight (kDa)

6.14

Isoelectric Point (pI)

46.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDRF1_N PF23788 67 - 533 1.2e-44 EDRF1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 4263
AatII GACGTC 1 cut(s) 393
Acc36I ACCTGC 1 cut(s) 4263
AccB1I GGYRCC 1 cut(s) 25
AccB7I CCANNNNNTGG 3 cut(s) 2508, 3463, 4173
AccBSI CCGCTC 1 cut(s) 161
AccII CGCG 1 cut(s) 196
AclWI GGATC 4 cut(s) 1172, 1253, 2190, 2317
AcoI YGGCCR 1 cut(s) 1086
AcuI CTGAAG 7 cut(s) 41, 248, 434, 1680, 1800, 2859, 4245
AcyI GRCGYC 1 cut(s) 390
AfaI GTAC 5 cut(s) 313, 746, 1707, 4004, 4161
AfiI CCNNNNNNNGG 7 cut(s) 2082, 2497, 2508, 2579, 3113, 3463, 4173
AflII CTTAAG 1 cut(s) 3497
AflIII ACRYGT 2 cut(s) 1936, 2962
AhlI ACTAGT 1 cut(s) 3686
AjnI CCWGG 6 cut(s) 673, 718, 848, 1049, 1261, 2061
AloI GAACNNNNNNTCC 2 cut(s) 1926, 1958
Alw21I GWGCWC 5 cut(s) 38, 193, 276, 857, 2441
Alw26I GTCTC 6 cut(s) 212, 529, 2828, 3334, 3425, 3461
Alw44I GTGCAC 1 cut(s) 272
AlwI GGATC 4 cut(s) 1172, 1253, 2190, 2317
AlwNI CAGNNNCTG 3 cut(s) 1652, 1874, 3548
Ama87I CYCGRG 2 cut(s) 48, 4154
ApaLI GTGCAC 1 cut(s) 272
ApeKI GCWGC 8 cut(s) 266, 1874, 1979, 2700, 2703, 3161, 3512, 3806
Asp700I GAANNNNTTC 3 cut(s) 1405, 2163, 2587
AspLEI GCGC 4 cut(s) 810, 1531, 2028, 2763
AspS9I GGNCC 7 cut(s) 223, 346, 500, 2059, 2499, 3020, 3104
AsuHPI GGTGA 6 cut(s) 2135, 2364, 2554, 2927, 3797, 4142
AsuNHI GCTAGC 1 cut(s) 1975
AvaI CYCGRG 2 cut(s) 48, 4154
AvaII GGWCC 4 cut(s) 223, 2059, 3020, 3104
BaeGI GKGCMC 3 cut(s) 276, 2661, 2999
BaeI ACNNNNGTAYC 2 cut(s) 3630, 3663
BalI TGGCCA 1 cut(s) 1088
BanI GGYRCC 1 cut(s) 25
BanII GRGCYC 4 cut(s) 38, 236, 857, 2441
BbsI GAAGAC 3 cut(s) 2337, 3176, 3385
Bbv12I GWGCWC 5 cut(s) 38, 193, 276, 857, 2441
BbvI GCAGC 8 cut(s) 253, 1861, 1966, 2690, 2712, 3148, 3524, 3793
BceAI ACGGC 3 cut(s) 223, 418, 1231
BcgI CGANNNNNNTGC 4 cut(s) 377, 411, 581, 615
BciT130I CCWGG 6 cut(s) 675, 720, 850, 1051, 1263, 2063
BciVI GTATCC 3 cut(s) 1115, 4276, 4276
BclI TGATCA 2 cut(s) 1753, 3334
BcoDI GTCTC 6 cut(s) 212, 529, 2828, 3334, 3425, 3461
BcuI ACTAGT 1 cut(s) 3686
BfaI CTAG 8 cut(s) 333, 1494, 1523, 1976, 2690, 3687, 3693, 3869
BfmI CTRYAG 5 cut(s) 1629, 1905, 2674, 3108, 3976
BfrI CTTAAG 1 cut(s) 3497
BfuAI ACCTGC 1 cut(s) 4263
BfuI GTATCC 3 cut(s) 1115, 4276, 4276
BglII AGATCT 2 cut(s) 420, 2431
Bme1390I CCNGG 6 cut(s) 675, 720, 850, 1051, 1263, 2063
Bme18I GGWCC 4 cut(s) 223, 2059, 3020, 3104
BmeT110I CYCGRG 2 cut(s) 48, 4154
BmgT120I GGNCC 7 cut(s) 223, 346, 500, 2059, 2499, 3020, 3104
BmiI GGNNCC 6 cut(s) 27, 46, 115, 1235, 1470, 3021
BmrFI CCNGG 6 cut(s) 675, 720, 850, 1051, 1263, 2063
BmrI ACTGGG 2 cut(s) 507, 3257
BmtI GCTAGC 1 cut(s) 1979
BmuI ACTGGG 2 cut(s) 507, 3257
BpiI GAAGAC 3 cut(s) 2337, 3176, 3385
BplI GAGNNNNNCTC 6 cut(s) 2462, 2494, 3536, 3568, 4015, 4047
BpmI CTGGAG 5 cut(s) 384, 702, 1284, 2425, 3684
Bpu10I CCTNAGC 1 cut(s) 2550
BpuEI CTTGAG 4 cut(s) 1064, 1351, 1983, 3407
Bsa29I ATCGAT 1 cut(s) 1700
BsaAI YACGTR 1 cut(s) 3603
BsaBI GATNNNNATC 1 cut(s) 2847
BsaHI GRCGYC 1 cut(s) 390
BsaI GGTCTC 2 cut(s) 3425, 3461
BsaWI WCCGGW 2 cut(s) 1931, 3191
Bsc4I CCNNNNNNNGG 7 cut(s) 2082, 2497, 2508, 2579, 3113, 3463, 4173
Bse1I ACTGG 6 cut(s) 502, 1728, 2520, 3033, 3252, 3667
Bse3DI GCAATG 5 cut(s) 261, 393, 2818, 3247, 4077
Bse8I GATNNNNATC 1 cut(s) 2847
BseBI CCWGG 6 cut(s) 675, 720, 850, 1051, 1263, 2063
BseCI ATCGAT 1 cut(s) 1700
BseJI GATNNNNATC 1 cut(s) 2847
BseLI CCNNNNNNNGG 7 cut(s) 2082, 2497, 2508, 2579, 3113, 3463, 4173
BseMI GCAATG 5 cut(s) 261, 393, 2818, 3247, 4077
BseMII CTCAG 8 cut(s) 701, 1071, 1161, 2029, 2426, 2856, 3567, 3599
BseNI ACTGG 6 cut(s) 502, 1728, 2520, 3033, 3252, 3667
BseSI GKGCMC 3 cut(s) 276, 2661, 2999
BseXI GCAGC 8 cut(s) 253, 1861, 1966, 2690, 2712, 3148, 3524, 3793
BseYI CCCAGC 3 cut(s) 1289, 4207, 4251
BsgI GTGCAG 3 cut(s) 1136, 1542, 1860
Bsh1236I CGCG 1 cut(s) 196
BshNI GGYRCC 1 cut(s) 25
BshVI ATCGAT 1 cut(s) 1700
BsiHKAI GWGCWC 5 cut(s) 38, 193, 276, 857, 2441
BsiHKCI CYCGRG 2 cut(s) 48, 4154
BsiSI CCGG 4 cut(s) 245, 288, 1932, 3192
BslFI GGGAC 4 cut(s) 711, 1438, 1743, 3033
BslI CCNNNNNNNGG 7 cut(s) 2082, 2497, 2508, 2579, 3113, 3463, 4173
BsmAI GTCTC 6 cut(s) 212, 529, 2828, 3334, 3425, 3461
BsmBI CGTCTC 2 cut(s) 212, 529
BsmFI GGGAC 4 cut(s) 711, 1438, 1743, 3033
BsmI GAATGC 3 cut(s) 210, 592, 3317
Bso31I GGTCTC 2 cut(s) 3425, 3461
BsoBI CYCGRG 2 cut(s) 48, 4154
Bsp1286I GDGCHC 8 cut(s) 38, 193, 236, 276, 857, 2441, 2661, 2999
Bsp19I CCATGG 1 cut(s) 3966
BspCNI CTCAG 8 cut(s) 700, 1072, 1162, 2028, 2427, 2857, 3568, 3600
BspDI ATCGAT 1 cut(s) 1700
BspFNI CGCG 1 cut(s) 196
BspHI TCATGA 1 cut(s) 1099
BspLI GGNNCC 6 cut(s) 27, 46, 115, 1235, 1470, 3021
BspMAI CTGCAG 3 cut(s) 1909, 2678, 3112
BspMI ACCTGC 1 cut(s) 4263
BspOI GCTAGC 1 cut(s) 1979
BspPI GGATC 4 cut(s) 1172, 1253, 2190, 2317
BspQI GCTCTTC 2 cut(s) 224, 1593
BspT107I GGYRCC 1 cut(s) 25
BspTI CTTAAG 1 cut(s) 3497
BspTNI GGTCTC 2 cut(s) 3425, 3461
BsrBI CCGCTC 1 cut(s) 161
BsrDI GCAATG 5 cut(s) 261, 393, 2818, 3247, 4077
BsrI ACTGG 6 cut(s) 502, 1728, 2520, 3033, 3252, 3667
BssNI GRCGYC 1 cut(s) 390
BssT1I CCWWGG 6 cut(s) 279, 441, 2502, 3097, 3939, 3966
Bst2UI CCWGG 6 cut(s) 675, 720, 850, 1051, 1263, 2063
Bst6I CTCTTC 8 cut(s) 224, 507, 877, 1316, 1593, 1972, 2498, 3390
BstACI GRCGYC 1 cut(s) 390
BstAFI CTTAAG 1 cut(s) 3497
BstAPI GCANNNNNTGC 1 cut(s) 1490
BstBAI YACGTR 1 cut(s) 3603
BstDSI CCRYGG 2 cut(s) 194, 3966
BstFNI CGCG 1 cut(s) 196
BstHHI GCGC 4 cut(s) 810, 1531, 2028, 2763
BstMAI GTCTC 6 cut(s) 212, 529, 2828, 3334, 3425, 3461
BstNI CCWGG 6 cut(s) 675, 720, 850, 1051, 1263, 2063
BstNSI RCATGY 7 cut(s) 943, 1940, 2121, 2966, 3677, 3958, 4099
BstSCI CCNGG 6 cut(s) 673, 718, 848, 1049, 1261, 2061
BstSFI CTRYAG 5 cut(s) 1629, 1905, 2674, 3108, 3976
BstSLI GKGCMC 3 cut(s) 276, 2661, 2999
BstSNI TACGTA 1 cut(s) 3603
BstUI CGCG 1 cut(s) 196
BstV1I GCAGC 8 cut(s) 253, 1861, 1966, 2690, 2712, 3148, 3524, 3793
BstV2I GAAGAC 3 cut(s) 2337, 3176, 3385
BstX2I RGATCY 4 cut(s) 420, 1164, 1258, 2431
BstXI CCANNNNNNTGG 1 cut(s) 1038
BstYI RGATCY 4 cut(s) 420, 1164, 1258, 2431
Bsu15I ATCGAT 1 cut(s) 1700
BsuI GTATCC 3 cut(s) 1115, 4276, 4276
BsuTUI ATCGAT 1 cut(s) 1700
BtgI CCRYGG 2 cut(s) 194, 3966
BtsI GCAGTG 2 cut(s) 2731, 2740
BtsIMutI CAGTG 9 cut(s) 65, 189, 1470, 1650, 2513, 2731, 2740, 2961, 3761
BveI ACCTGC 1 cut(s) 4263
CaiI CAGNNNCTG 3 cut(s) 1652, 1874, 3548
CciI TCATGA 1 cut(s) 1099
CfoI GCGC 4 cut(s) 810, 1531, 2028, 2763
Cfr13I GGNCC 7 cut(s) 223, 346, 500, 2059, 2499, 3020, 3104
Cfr42I CCGCGG 1 cut(s) 197
ClaI ATCGAT 1 cut(s) 1700
CseI GACGC 1 cut(s) 490
Csp6I GTAC 5 cut(s) 312, 745, 1706, 4003, 4160
CviQI GTAC 5 cut(s) 312, 745, 1706, 4003, 4160
EaeI YGGCCR 1 cut(s) 1086
Eam1104I CTCTTC 8 cut(s) 224, 507, 877, 1316, 1593, 1972, 2498, 3390
EarI CTCTTC 8 cut(s) 224, 507, 877, 1316, 1593, 1972, 2498, 3390
Ecl136II GAGCTC 3 cut(s) 36, 855, 2439
Eco105I TACGTA 1 cut(s) 3603
Eco130I CCWWGG 6 cut(s) 279, 441, 2502, 3097, 3939, 3966
Eco147I AGGCCT 1 cut(s) 1049
Eco24I GRGCYC 4 cut(s) 38, 236, 857, 2441
Eco31I GGTCTC 2 cut(s) 3425, 3461
Eco32I GATATC 1 cut(s) 2894
Eco47I GGWCC 4 cut(s) 223, 2059, 3020, 3104
Eco53kI GAGCTC 3 cut(s) 36, 855, 2439
Eco57I CTGAAG 7 cut(s) 41, 248, 434, 1680, 1800, 2859, 4245
Eco88I CYCGRG 2 cut(s) 48, 4154
EcoICRI GAGCTC 3 cut(s) 36, 855, 2439
EcoO109I RGGNCCY 2 cut(s) 346, 3104
EcoRI GAATTC 2 cut(s) 1517, 2537
EcoRII CCWGG 6 cut(s) 673, 718, 848, 1049, 1261, 2061
EcoRV GATATC 1 cut(s) 2894
EcoT14I CCWWGG 6 cut(s) 279, 441, 2502, 3097, 3939, 3966
EcoT22I ATGCAT 4 cut(s) 594, 1816, 3463, 3937
EcoT38I GRGCYC 4 cut(s) 38, 236, 857, 2441
ErhI CCWWGG 6 cut(s) 279, 441, 2502, 3097, 3939, 3966
Esp3I CGTCTC 2 cut(s) 212, 529
FalI AAGNNNNNCTT 4 cut(s) 407, 439, 4225, 4257
FaqI GGGAC 4 cut(s) 711, 1438, 1743, 3033
FauI CCCGC 4 cut(s) 182, 243, 630, 1542
FauNDI CATATG 2 cut(s) 1744, 2800
FbaI TGATCA 2 cut(s) 1753, 3334
FriOI GRGCYC 4 cut(s) 38, 236, 857, 2441
FspBI CTAG 8 cut(s) 333, 1494, 1523, 1976, 2690, 3687, 3693, 3869
GlaI GCGC 4 cut(s) 809, 1530, 2027, 2762
GsaI CCCAGC 3 cut(s) 1293, 4211, 4255
GsuI CTGGAG 5 cut(s) 384, 702, 1284, 2425, 3684
HapII CCGG 4 cut(s) 245, 288, 1932, 3192
HgaI GACGC 1 cut(s) 490
HhaI GCGC 4 cut(s) 810, 1531, 2028, 2763
Hin1I GRCGYC 1 cut(s) 390
Hin6I GCGC 4 cut(s) 808, 1529, 2026, 2761
HinP1I GCGC 4 cut(s) 808, 1529, 2026, 2761
HindIII AAGCTT 5 cut(s) 609, 1782, 2241, 2271, 2948
HpaII CCGG 4 cut(s) 245, 288, 1932, 3192
HphI GGTGA 6 cut(s) 2135, 2364, 2554, 2927, 3797, 4142
Hpy166II GTNNAC 5 cut(s) 274, 747, 1640, 4162, 4365
Hpy188III TCNNGA 9 cut(s) 48, 360, 740, 1017, 1100, 1241, 2327, 2426, 3708
Hpy8I GTNNAC 5 cut(s) 274, 747, 1640, 4162, 4365
Hpy99I CGWCG 6 cut(s) 25, 101, 217, 244, 392, 899
HpyCH4IV ACGT 3 cut(s) 390, 538, 3602
HpySE526I ACGT 3 cut(s) 390, 538, 3602
Hsp92I GRCGYC 1 cut(s) 390
HspAI GCGC 4 cut(s) 808, 1529, 2026, 2761
Ksp22I TGATCA 2 cut(s) 1753, 3334
KspI CCGCGG 1 cut(s) 197
LguI GCTCTTC 2 cut(s) 224, 1593
Lsp1109I GCAGC 8 cut(s) 253, 1861, 1966, 2690, 2712, 3148, 3524, 3793
MaeI CTAG 8 cut(s) 333, 1494, 1523, 1976, 2690, 3687, 3693, 3869
MaeII ACGT 3 cut(s) 390, 538, 3602
MaeIII GTNAC 9 cut(s) 890, 953, 1014, 1024, 1648, 1774, 2147, 3152, 3362
MbiI CCGCTC 1 cut(s) 161
MfeI CAATTG 2 cut(s) 456, 4340
MflI RGATCY 4 cut(s) 420, 1164, 1258, 2431
MhlI GDGCHC 8 cut(s) 38, 193, 236, 276, 857, 2441, 2661, 2999
MlsI TGGCCA 1 cut(s) 1088
MluNI TGGCCA 1 cut(s) 1088
MlyI GAGTC 7 cut(s) 875, 1420, 2228, 3675, 3930, 4121, 4127
MmeI TCCRAC 7 cut(s) 236, 454, 2192, 2500, 3094, 3940, 4056
Mox20I TGGCCA 1 cut(s) 1088
Mph1103I ATGCAT 4 cut(s) 594, 1816, 3463, 3937
MroXI GAANNNNTTC 3 cut(s) 1405, 2163, 2587
MscI TGGCCA 1 cut(s) 1088
MseI TTAA 9 cut(s) 527, 756, 1034, 1682, 2007, 2100, 2634, 3498, 4334
MslI CAYNNNNRTG 3 cut(s) 708, 1197, 4065
Msp20I TGGCCA 1 cut(s) 1088
MspA1I CMGCKG 3 cut(s) 196, 404, 2703
MspCI CTTAAG 1 cut(s) 3497
MspI CCGG 4 cut(s) 245, 288, 1932, 3192
MspR9I CCNGG 6 cut(s) 675, 720, 850, 1051, 1263, 2063
MunI CAATTG 2 cut(s) 456, 4340
Mva1269I GAATGC 3 cut(s) 210, 592, 3317
MvaI CCWGG 6 cut(s) 675, 720, 850, 1051, 1263, 2063
MvnI CGCG 1 cut(s) 196
NcoI CCATGG 1 cut(s) 3966
NdeI CATATG 2 cut(s) 1744, 2800
NheI GCTAGC 1 cut(s) 1975
NlaIV GGNNCC 6 cut(s) 27, 46, 115, 1235, 1470, 3021
NmuCI GTSAC 4 cut(s) 953, 1014, 1648, 2147
NsiI ATGCAT 4 cut(s) 594, 1816, 3463, 3937
NspI RCATGY 7 cut(s) 943, 1940, 2121, 2966, 3677, 3958, 4099
PaeI GCATGC 2 cut(s) 943, 4099
PagI TCATGA 1 cut(s) 1099
PaqCI CACCTGC 1 cut(s) 4263
PceI AGGCCT 1 cut(s) 1049
PciI ACATGT 2 cut(s) 1936, 2962
PciSI GCTCTTC 2 cut(s) 224, 1593
PcsI WCGNNNNNNNCGW 1 cut(s) 26
PctI GAATGC 3 cut(s) 210, 592, 3317
PdmI GAANNNNTTC 3 cut(s) 1405, 2163, 2587
PflFI GACNNNGTC 1 cut(s) 1061
PflMI CCANNNNNTGG 3 cut(s) 2508, 3463, 4173
PfoI TCCNGGA 2 cut(s) 848, 1261
PleI GAGTC 7 cut(s) 874, 1420, 2227, 3674, 3929, 4121, 4127
PpsI GAGTC 7 cut(s) 874, 1420, 2227, 3674, 3929, 4121, 4127
Ppu21I YACGTR 1 cut(s) 3603
PpuMI RGGWCCY 1 cut(s) 3104
PscI ACATGT 2 cut(s) 1936, 2962
Psp124BI GAGCTC 3 cut(s) 38, 857, 2441
Psp5II RGGWCCY 1 cut(s) 3104
Psp6I CCWGG 6 cut(s) 673, 718, 848, 1049, 1261, 2061
PspFI CCCAGC 3 cut(s) 1289, 4207, 4251
PspGI CCWGG 6 cut(s) 673, 718, 848, 1049, 1261, 2061
PspN4I GGNNCC 6 cut(s) 27, 46, 115, 1235, 1470, 3021
PspPI GGNCC 7 cut(s) 223, 346, 500, 2059, 2499, 3020, 3104
PspPPI RGGWCCY 1 cut(s) 3104
PstI CTGCAG 3 cut(s) 1909, 2678, 3112
PstNI CAGNNNCTG 3 cut(s) 1652, 1874, 3548
PsuI RGATCY 4 cut(s) 420, 1164, 1258, 2431
PsyI GACNNNGTC 1 cut(s) 1061
PvuII CAGCTG 2 cut(s) 404, 2703
RsaI GTAC 5 cut(s) 313, 746, 1707, 4004, 4161
RsaNI GTAC 5 cut(s) 312, 745, 1706, 4003, 4160
RseI CAYNNNNRTG 3 cut(s) 708, 1197, 4065
SacI GAGCTC 3 cut(s) 38, 857, 2441
SacII CCGCGG 1 cut(s) 197
SapI GCTCTTC 2 cut(s) 224, 1593
SaqAI TTAA 9 cut(s) 527, 756, 1034, 1682, 2007, 2100, 2634, 3498, 4334
Sau96I GGNCC 7 cut(s) 223, 346, 500, 2059, 2499, 3020, 3104
SchI GAGTC 7 cut(s) 875, 1420, 2228, 3675, 3930, 4121, 4127
ScrFI CCNGG 6 cut(s) 675, 720, 850, 1051, 1263, 2063
SduI GDGCHC 8 cut(s) 38, 193, 236, 276, 857, 2441, 2661, 2999
SfcI CTRYAG 5 cut(s) 1629, 1905, 2674, 3108, 3976
Sfr303I CCGCGG 1 cut(s) 197
SgrBI CCGCGG 1 cut(s) 197
SinI GGWCC 4 cut(s) 223, 2059, 3020, 3104
SmiMI CAYNNNNRTG 3 cut(s) 708, 1197, 4065
SmlI CTYRAG 5 cut(s) 1043, 1366, 1998, 3422, 3497
SmoI CTYRAG 5 cut(s) 1043, 1366, 1998, 3422, 3497
SnaBI TACGTA 1 cut(s) 3603
SpeI ACTAGT 1 cut(s) 3686
SphI GCATGC 2 cut(s) 943, 4099
SseBI AGGCCT 1 cut(s) 1049
SspI AATATT 1 cut(s) 2588
SspMI CTAG 8 cut(s) 333, 1494, 1523, 1976, 2690, 3687, 3693, 3869
SstI GAGCTC 3 cut(s) 38, 857, 2441
StuI AGGCCT 1 cut(s) 1049
StyD4I CCNGG 6 cut(s) 673, 718, 848, 1049, 1261, 2061
StyI CCWWGG 6 cut(s) 279, 441, 2502, 3097, 3939, 3966
TaiI ACGT 3 cut(s) 393, 541, 3605
TaqII GACCGA 1 cut(s) 3037
TatI WGTACW 1 cut(s) 744
TauI GCSGC 2 cut(s) 114, 287
Tru1I TTAA 9 cut(s) 527, 756, 1034, 1682, 2007, 2100, 2634, 3498, 4334
Tru9I TTAA 9 cut(s) 527, 756, 1034, 1682, 2007, 2100, 2634, 3498, 4334
TscAI CASTG 9 cut(s) 65, 189, 1470, 1657, 2520, 2731, 2740, 2961, 3768
TseFI GTSAC 4 cut(s) 953, 1014, 1648, 2147
TseI GCWGC 8 cut(s) 266, 1874, 1979, 2700, 2703, 3161, 3512, 3806
Tsp45I GTSAC 4 cut(s) 953, 1014, 1648, 2147
TspGWI ACGGA 3 cut(s) 230, 236, 1872
TspRI CASTG 9 cut(s) 65, 189, 1470, 1657, 2520, 2731, 2740, 2961, 3768
Tth111I GACNNNGTC 1 cut(s) 1061
Van91I CCANNNNNTGG 3 cut(s) 2508, 3463, 4173
Vha464I CTTAAG 1 cut(s) 3497
VneI GTGCAC 1 cut(s) 272
VpaK11BI GGWCC 4 cut(s) 223, 2059, 3020, 3104
XceI RCATGY 7 cut(s) 943, 1940, 2121, 2966, 3677, 3958, 4099
XmnI GAANNNNTTC 3 cut(s) 1405, 2163, 2587
XspI CTAG 8 cut(s) 333, 1494, 1523, 1976, 2690, 3687, 3693, 3869
ZraI GACGTC 1 cut(s) 391
Zsp2I ATGCAT 4 cut(s) 594, 1816, 3463, 3937
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.