MD05G1041500.v1.1

transcription, DNA-templated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011317
Physical Location & Seq
Forward (+)
6977277 .. 6984032
6756 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1041500.v1.1.491

Sequence Viewer

Length: 4383 bp
ATGGAGAAATCAGGGGTGTCATCGGTACCGTTGAGCTCCGATGGCTCTCGAGAGCTTCAGTGCATCGGCAAGTTAGAAATCGAAAGGCCGAACCCCGTCGGCTTCCTCTGCGGCTCCATCCCTGTTCCCACCGATAAAGCCTTCCACTCTTTCGATTCCGCTCTCATTCCCTCCCGCCAAACAGTGAGTGCTCCACGGTATCGAATGCTTCCGACAGAGACCGACCTCAAGAGCCCGCCGCTCCTTTCGCCCGACAAAGTTCTTCCCATTGCTGCCGTGCACTCCAAGGCCGCCGGAGATATAGCTTGGGATGGTGGCACTGTTACATCAAATCTCACTAGAAAATGCGAGGCCCTTGCGGTGTCTGGATTGGTTGAGTATGGGGATGAGATCGACGTCATTGCTCCAGCTGACATTCTGAAGCAGATCTTCAAAATGCCTTATTCCAAGGCTCGATTATCAATTGCAGTTCAGCGTGTTGATCAGACGCTTGTTCTTAATACTGGGCCTGACATTGAAGAGGGGGAAAAGTTAATAAGAAGACGTAAAAATCAATCAAAAGGTGCAGATCAGTCTTTGTTTTTGAATTTTGCTATGCATTCTGTTCGGATGGAAGCTTGTGATTGCCCGCCAACTCATCATGTCCCATCACGAGAGCAATCAAACTCCTCTGTTCTTCCTGGGGCAAATACTCAGTTTGTGGGACAGCACAATGATGTTGCTCCAGGTGGAAAATCAAATTGTCCAGAATACGAACAGGTTAAAGGGGATGATTTCTTTATGGCAAAAAAGAAGGATAAGAAAAATAAGGGGTGCAATCCTGTCAAAAAGGCTTCACAAGTTGGCGAGAAGTCCAGGAACTCAATGCAAGAGTCTGAAAAACATAGAAGAGCTGGTAACGATGGGTTTCTAAGGGTTTTGTTTTGGCAATTTCACAATTTCCGCATGCTCATAGGGAGTGACTTGCTTCTGTTCAGTAATGAAAAATATGTTGCTGTTAGCTTGCATCTCTGGGATGTGTCACGACAGGTTACACCATTAACTTGGCTTGAAGCCTGGCTTGACAATGTCATGGCAAGTGTGCCTGAGATGGCCATTTGTTATCATGAAAACGGTGTTGTGCAGGGATACGAGCTTTTGAAAACAGATGATATATTTCTACTAAAAGGGATCTCTGAGGAAGGCGCTCCTGCTTTTCATCCTTATGTGGTGCAGCAAAATGGCCTTTCCGTTTTGAGGTTCCTTCAAGAAAACTGCAAGCAAGATCCTGGAGCTTATTGGCTGTATAAAAATGCTGGGGAAGATGTCATTCAACTCTTTGATCTTTCTGTTATTCCCAAGAACTGTTCTTCTAATGAATGTGATGATAGCTCAAGCTCCCTTCCTTCTATATTGCATCGAGGAAGAAGTGATTCTCTGTATTCATTGGGGACTCTCTTGTATAGAAGTGCTCACAGGCTTTCACTTTCAGTGGCTCCAAATAATATGGCAAAGTGTGCCAGGTTCTTCCAAAAATGTTTGGAATTTCTAGATGCGCCAGATCATCTGGTTGTGCGTGCATCTGCACATGAACAATTTGCGAGGCTTATTTTGAACCACGATGAAGAGCTGGAATTAGCGTCTGATGATCTGCCTGTAGGATGTGAACTTACTGTTACTGATGCTGAAGAAGATTCTTTGGACTTTTTAAGTAGCATTTCTGAATCAACAGTCCATGAAGAACCCAGCCTAGTGGGAGAGGAGAAAGCATATGACTATGGTCAAAATGTTGAAGATTTAGTAACTGAAGCTTCGGTAAAGATGACTTTAGAAGCAAATGCATATTCTCCAAGAAAATTGATAGCAGAAGGTAGCATGGACTACGGAGATTTGACAGAAGCTGTACCAAATTCCTCTGGTATTGAAAGCTCTGCAGTTTGCAAATTGCCTGCAACAACCGGACATGTAGTTCAAATTGTTGCTGAACCAATCTCTTCTAAGCTAGCTGCAATACATCATGTTTCTCAAGCCATTAAATCTCTCAGATGGATGCGCCAACTGCAAACTACTGAATCCAAGTTAATGGACCAGGAGAGTGAAACCCATGATAGGCCACCTTCATCTGTTAATCTTTCTGTTTGTGCATGTGGTGATGCTGACTGTATCGAAGTGTGTGACATTAGAGAATGGCTTCCAACATCAACATTGGACCACAAGTTGTGGAAACTTGTTCTTTTGCTTGGAGAGTCATATTTGGCGCTTGGACAAGCCTATAAGGAGGATTGTCAGTTGCATCAAGCTTTGAAGGTTGTAGAATTGGCATGTTCTGTATATGGATCAATGCCTCAGCATCTTGAAGACACAAAATTCATTTCTTCCATGAATAGTTTCTTCTCATCACCAACAAAATTCAATTATACTAATAAAAAGACTACATCATCTATTAGTGATCAGGAAGATCTGAGCTCCAGCTCCACTCATGGTTGTCTTTCTTTCGAGCAGTTCTCGTCCATTTACCTCTTCTGGGCCAAGGCATGGACACTGGTTGGAGATGTTTATGTTGAATTCCATTTGGCTAAGGATAGTGTGATCTGTCCACCAGTAGAGAGAAAATATTCTATTAGAGAGTTGAAAGTTTCATCTGAGGTTGTCAAAGAAGTTAAAAGGCTCAAGAAGAAGTTGGGGCAGCACACGCAAAACTGCAGTTCATGTTCTCTAGTAAATTGCAGCTGCCAGAGTGATAGGGCAAGCAGTGGTAGCAGTGCAAGTAGTAGTGGTGATATGAGCTCAGTAAATTCTGGCAGGAAATACAGTAAAAGACCACATGCTAAGAGCAATGCCTTCCCCCTTTTAAGAGACCCAGAAGATGATAGTCTTTGTTTAAAAATGGAGAATGGAAAGGTTTCTGATTGCGGATATCTAAACCAAAAGAGAAACGGCGAAACCATTGTACAGTCTTCGAGTACAGATAAGTTTGTAGGGAAACATGTGGAATCTGGTAGTTCTGGCAATTTAGAAGGCACTTTAGGAGTGGATGACATGGGGCCAATACTGGCCTCCCAAACTAATTCTTCTCTGAGAGAAACAATGAAATTGAAAAATGGAGGGATATTCAAGTACCTTGGTGGTCCTGCAGTTAGAGATGCTGAAAACAATCTTTCAGAAGCCCTATGCTGTTATGGAGAAGCTACAAAAGCATTAGGTGGTCTTCCATCCAGTTCTGCAGAACTACAATCTATAAGGAAAAAGAAAGGGTGGGTATGCAATGAACTGGGTCGAAACAGGCTTGAAAAGAAAGAATTAAAAAAAGCTGAACTTGCTTTTGTAGATGCTATAAAAGCATTCAGAGAAGTCTCTGATCATACCAACATCATATTGATAAACTGCAACTTGGGGCATGGAAGACGGGCATTGGCAGAAGAGATGGTATCAAAGATTGATAATCTCAAGGGGCATCCGATCTTTCATAACGCATACAACCAAGCATTGGAGACTGCCAAGTTAGAATATAGTGAATCCCTTAAGTATTATGGGGCAGCAAAAGCGGAACTCGATGCTTTTGTTGAAGAGGCTGGCTCTGATTTAAACAGTTTGAGGACTGAGGTATATACACAGTTTGCTCATACGTATCTGAGGCTAGGAATGCTCTTGGCAAGAGAAGATACAACTGTTGATGTCTATGAAACTGGAGTGATGGTAGATGTGCGACGTTCCACCAGCCCTAGTGGCAGAAAATCAAGAAAACAATCACGAAAGCATGAGATTTCAGCCAATGATGCCATCCGGGAGGCACTGTCTGTTTATGAGTCATTAGGTGAATTGCGGAAACAGGAGGCTGCATATTCATATTTTCAGTTGGCTTGCTACCACAGGGATCATTGTTTGAAATTCTTAGAGCCAGATCATAAGAAAAACAACTTATCCAAAGGTGAAAACACAATTATCCAACGGGTCAAGCAATATGCATCCTTGGCAGAGAGGAACATGCAAAAGGCCATGGATTTTTATGGCCCAAAAACACATCCTACCATGTACTTAACTATTCTTATAGAGAGATCAGCTCTTTCATTGAGCCTTTCAAGCCCTTTACACTCAAATGCAATGTTGGAATCAGCTTTGTCTTGCTTGCTTGAAGGGCGGTATGTGTCAGAAACAGACTCGGACTGTTTGAAAACCAACCACCCCGAGGTACATGCCAAGTTTTGGAATCAGTTGCAGATGCTTTTGAAGAAGTTGCTGGGGATGGCGCTTTCCTCACGCGCAAGCAAATCTTCGGTTTCTCAGCCAGCTTCAGTATCCAACAGGTGTGGGGATACTGAAAAACTTAGGCAGCTTTACAAATTCTCTCTCAATTCCACAAAGTTGAATCAATTGGATGCTATGCACTTCTTGTGGACATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1461

Amino Acids

161.87

Weight (kDa)

6.25

Isoelectric Point (pI)

44.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDRF1_N PF23788 286 - 556 3.8e-37 EDRF1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 399
Acc65I GGTACC 1 cut(s) 25
AccB1I GGYRCC 1 cut(s) 25
AccB7I CCANNNNNTGG 3 cut(s) 2514, 3466, 4182
AccBSI CCGCTC 2 cut(s) 161, 241
AccII CGCG 1 cut(s) 4239
AclWI GGATC 4 cut(s) 1178, 1259, 2323, 3861
AcoI YGGCCR 1 cut(s) 1092
AcsI RAATTY 9 cut(s) 586, 1523, 1888, 2345, 2387, 2543, 2773, 3866, 4319
AcuI CTGAAG 5 cut(s) 41, 440, 1686, 1806, 4254
AcyI GRCGYC 1 cut(s) 396
AfaI GTAC 7 cut(s) 27, 1884, 2931, 2944, 3098, 4013, 4170
AfiI CCNNNNNNNGG 7 cut(s) 1236, 2088, 2503, 2514, 3466, 4165, 4182
AflII CTTAAG 1 cut(s) 3500
AflIII ACRYGT 2 cut(s) 1942, 2965
AjnI CCWGG 7 cut(s) 679, 724, 854, 1055, 1267, 1499, 2067
AloI GAACNNNNNNTCC 2 cut(s) 1932, 1964
Alw21I GWGCWC 6 cut(s) 38, 193, 282, 1453, 2447, 2768
Alw26I GTCTC 4 cut(s) 212, 2829, 3337, 3464
Alw44I GTGCAC 1 cut(s) 278
AlwI GGATC 4 cut(s) 1178, 1259, 2323, 3861
AlwNI CAGNNNCTG 1 cut(s) 1880
Ama87I CYCGRG 2 cut(s) 48, 4163
ApaLI GTGCAC 1 cut(s) 278
ApeKI GCWGC 9 cut(s) 272, 1213, 1985, 2665, 2706, 2709, 3515, 3815, 4309
ApoI RAATTY 9 cut(s) 586, 1523, 1888, 2345, 2387, 2543, 2773, 3866, 4319
Asp700I GAANNNNTTC 5 cut(s) 1411, 2169, 2366, 2593, 2881
Asp718I GGTACC 1 cut(s) 25
AspLEI GCGC 6 cut(s) 1187, 1537, 2034, 2239, 4228, 4241
AspS9I GGNCC 8 cut(s) 352, 506, 2065, 2188, 2505, 3023, 3107, 3989
AsuC2I CCSGG 1 cut(s) 3764
AsuHPI GGTGA 5 cut(s) 2141, 2370, 2768, 3806, 3920
AsuNHI GCTAGC 1 cut(s) 1981
AvaI CYCGRG 2 cut(s) 48, 4163
AvaII GGWCC 3 cut(s) 2065, 2188, 3107
BaeGI GKGCMC 1 cut(s) 282
BaeI ACNNNNGTAYC 2 cut(s) 3633, 3666
BalI TGGCCA 1 cut(s) 1094
BanI GGYRCC 1 cut(s) 25
BanII GRGCYC 4 cut(s) 38, 236, 2447, 2768
BauI CACGAG 1 cut(s) 651
BbsI GAAGAC 5 cut(s) 547, 2343, 2928, 3179, 3388
Bbv12I GWGCWC 6 cut(s) 38, 193, 282, 1453, 2447, 2768
BbvCI CCTCAGC 1 cut(s) 2325
BbvI GCAGC 9 cut(s) 259, 1225, 1972, 2677, 2696, 2718, 3527, 3802, 4321
BceAI ACGGC 2 cut(s) 260, 2932
BcgI CGANNNNNNTGC 8 cut(s) 338, 372, 383, 417, 587, 621, 4154, 4188
BciT130I CCWGG 7 cut(s) 681, 726, 856, 1057, 1269, 1501, 2069
BciVI GTATCC 3 cut(s) 1121, 4285, 4285
BclI TGATCA 3 cut(s) 481, 2428, 3337
BcnI CCSGG 1 cut(s) 3764
BcoDI GTCTC 4 cut(s) 212, 2829, 3337, 3464
BfaI CTAG 7 cut(s) 339, 1529, 1730, 1982, 2696, 3617, 3702
BfmI CTRYAG 5 cut(s) 1635, 1911, 2680, 3111, 3201
BfoI RGCGCY 3 cut(s) 1188, 2240, 4229
BfrI CTTAAG 1 cut(s) 3500
BfuI GTATCC 3 cut(s) 1121, 4285, 4285
BglI GCCNNNNNGGC 1 cut(s) 3705
BglII AGATCT 2 cut(s) 426, 2437
Bme1390I CCNGG 8 cut(s) 681, 726, 856, 1057, 1269, 1501, 2069, 3764
Bme18I GGWCC 3 cut(s) 2065, 2188, 3107
BmeT110I CYCGRG 2 cut(s) 48, 4163
BmgT120I GGNCC 8 cut(s) 352, 506, 2065, 2188, 2505, 3023, 3107, 3989
BmiI GGNNCC 5 cut(s) 27, 115, 1241, 1476, 3024
BmrFI CCNGG 8 cut(s) 681, 726, 856, 1057, 1269, 1501, 2069, 3764
BmrI ACTGGG 2 cut(s) 513, 3260
BmtI GCTAGC 1 cut(s) 1985
BmuI ACTGGG 2 cut(s) 513, 3260
BoxI GACNNNNGTC 1 cut(s) 1758
BpiI GAAGAC 5 cut(s) 547, 2343, 2928, 3179, 3388
BplI GAGNNNNNCTC 6 cut(s) 2468, 2500, 3539, 3571, 4024, 4056
BpmI CTGGAG 5 cut(s) 390, 708, 1290, 2431, 3687
Bpu10I CCTNAGC 2 cut(s) 2325, 2556
BpuEI CTTGAG 5 cut(s) 212, 1357, 1989, 2633, 3410
BpuMI CCSGG 1 cut(s) 3764
BsaAI YACGTR 1 cut(s) 3606
BsaHI GRCGYC 1 cut(s) 396
BsaI GGTCTC 2 cut(s) 212, 2829
BsaJI CCNNGG 9 cut(s) 194, 285, 447, 680, 2508, 3100, 3948, 3975, 4164
BsaWI WCCGGW 1 cut(s) 1937
BsaXI ACNNNNNCTCC 4 cut(s) 3660, 3690, 3758, 3788
Bsc4I CCNNNNNNNGG 7 cut(s) 1236, 2088, 2503, 2514, 3466, 4165, 4182
Bse1I ACTGG 7 cut(s) 508, 2526, 2579, 3036, 3195, 3255, 3670
Bse3DI GCAATG 5 cut(s) 267, 399, 2821, 3250, 4086
BseBI CCWGG 7 cut(s) 681, 726, 856, 1057, 1269, 1501, 2069
BseDI CCNNGG 9 cut(s) 194, 285, 447, 680, 2508, 3100, 3948, 3975, 4164
BseLI CCNNNNNNNGG 7 cut(s) 1236, 2088, 2503, 2514, 3466, 4165, 4182
BseMI GCAATG 5 cut(s) 267, 399, 2821, 3250, 4086
BseNI ACTGG 7 cut(s) 508, 2526, 2579, 3036, 3195, 3255, 3670
BseRI GAGGAG 2 cut(s) 658, 1754
BseSI GKGCMC 1 cut(s) 282
BseXI GCAGC 9 cut(s) 259, 1225, 1972, 2677, 2696, 2718, 3527, 3802, 4321
BseYI CCCAGC 3 cut(s) 1295, 1724, 4216
BsgI GTGCAG 4 cut(s) 585, 1142, 1232, 1548
Bsh1236I CGCG 1 cut(s) 4239
BshNI GGYRCC 1 cut(s) 25
BsiHKAI GWGCWC 6 cut(s) 38, 193, 282, 1453, 2447, 2768
BsiHKCI CYCGRG 2 cut(s) 48, 4163
BsiSI CCGG 3 cut(s) 294, 1938, 3763
BslFI GGGAC 3 cut(s) 629, 717, 1444
BslI CCNNNNNNNGG 7 cut(s) 1236, 2088, 2503, 2514, 3466, 4165, 4182
BsmAI GTCTC 4 cut(s) 212, 2829, 3337, 3464
BsmFI GGGAC 3 cut(s) 629, 717, 1444
BsmI GAATGC 4 cut(s) 210, 598, 3320, 3627
Bso31I GGTCTC 2 cut(s) 212, 2829
BsoBI CYCGRG 2 cut(s) 48, 4163
Bsp1286I GDGCHC 7 cut(s) 38, 193, 236, 282, 1453, 2447, 2768
Bsp1407I TGTACA 1 cut(s) 2929
Bsp19I CCATGG 1 cut(s) 3975
BspFNI CGCG 1 cut(s) 4239
BspHI TCATGA 1 cut(s) 1105
BspLI GGNNCC 5 cut(s) 27, 115, 1241, 1476, 3024
BspMAI CTGCAG 4 cut(s) 1915, 2684, 3115, 3205
BspOI GCTAGC 1 cut(s) 1985
BspPI GGATC 4 cut(s) 1178, 1259, 2323, 3861
BspQI GCTCTTC 2 cut(s) 883, 1599
BspT107I GGYRCC 1 cut(s) 25
BspTI CTTAAG 1 cut(s) 3500
BspTNI GGTCTC 2 cut(s) 212, 2829
BsrBI CCGCTC 2 cut(s) 161, 241
BsrDI GCAATG 5 cut(s) 267, 399, 2821, 3250, 4086
BsrGI TGTACA 1 cut(s) 2929
BsrI ACTGG 7 cut(s) 508, 2526, 2579, 3036, 3195, 3255, 3670
BssECI CCNNGG 9 cut(s) 194, 285, 447, 680, 2508, 3100, 3948, 3975, 4164
BssNI GRCGYC 1 cut(s) 396
BssSI CACGAG 1 cut(s) 651
BssT1I CCWWGG 6 cut(s) 285, 447, 2508, 3100, 3948, 3975
Bst2BI CACGAG 1 cut(s) 651
Bst2UI CCWGG 7 cut(s) 681, 726, 856, 1057, 1269, 1501, 2069
Bst6I CTCTTC 7 cut(s) 513, 883, 1599, 1978, 2504, 3393, 3540
BstACI GRCGYC 1 cut(s) 396
BstAFI CTTAAG 1 cut(s) 3500
BstAPI GCANNNNNTGC 1 cut(s) 1496
BstAUI TGTACA 1 cut(s) 2929
BstBAI YACGTR 1 cut(s) 3606
BstDSI CCRYGG 2 cut(s) 194, 3975
BstFNI CGCG 1 cut(s) 4239
BstH2I RGCGCY 3 cut(s) 1188, 2240, 4229
BstHHI GCGC 6 cut(s) 1187, 1537, 2034, 2239, 4228, 4241
BstMAI GTCTC 4 cut(s) 212, 2829, 3337, 3464
BstNI CCWGG 7 cut(s) 681, 726, 856, 1057, 1269, 1501, 2069
BstNSI RCATGY 8 cut(s) 949, 1946, 2127, 2304, 2807, 2969, 3967, 4175
BstPAI GACNNNNGTC 1 cut(s) 1758
BstSCI CCNGG 8 cut(s) 679, 724, 854, 1055, 1267, 1499, 2067, 3762
BstSFI CTRYAG 5 cut(s) 1635, 1911, 2680, 3111, 3201
BstSLI GKGCMC 1 cut(s) 282
BstSNI TACGTA 1 cut(s) 3606
BstUI CGCG 1 cut(s) 4239
BstV1I GCAGC 9 cut(s) 259, 1225, 1972, 2677, 2696, 2718, 3527, 3802, 4321
BstV2I GAAGAC 5 cut(s) 547, 2343, 2928, 3179, 3388
BstX2I RGATCY 4 cut(s) 426, 1170, 1264, 2437
BstXI CCANNNNNNTGG 3 cut(s) 1044, 1732, 2062
BstYI RGATCY 4 cut(s) 426, 1170, 1264, 2437
BsuI GTATCC 3 cut(s) 1121, 4285, 4285
BtgI CCRYGG 2 cut(s) 194, 3975
BtsI GCAGTG 2 cut(s) 2737, 2746
BtsIMutI CAGTG 8 cut(s) 65, 189, 318, 1476, 2519, 2737, 2746, 3770
CaiI CAGNNNCTG 1 cut(s) 1880
CciI TCATGA 1 cut(s) 1105
CfoI GCGC 6 cut(s) 1187, 1537, 2034, 2239, 4228, 4241
Cfr13I GGNCC 8 cut(s) 352, 506, 2065, 2188, 2505, 3023, 3107, 3989
CseI GACGC 2 cut(s) 496, 1608
Csp6I GTAC 7 cut(s) 26, 1883, 2930, 2943, 3097, 4012, 4169
CspCI CAANNNNNGTGG 2 cut(s) 4267, 4302
CviQI GTAC 7 cut(s) 26, 1883, 2930, 2943, 3097, 4012, 4169
DraI TTTAAA 2 cut(s) 2862, 3564
EaeI YGGCCR 1 cut(s) 1092
Eam1104I CTCTTC 7 cut(s) 513, 883, 1599, 1978, 2504, 3393, 3540
EarI CTCTTC 7 cut(s) 513, 883, 1599, 1978, 2504, 3393, 3540
Ecl136II GAGCTC 3 cut(s) 36, 2445, 2766
Eco105I TACGTA 1 cut(s) 3606
Eco130I CCWWGG 6 cut(s) 285, 447, 2508, 3100, 3948, 3975
Eco24I GRGCYC 4 cut(s) 38, 236, 2447, 2768
Eco31I GGTCTC 2 cut(s) 212, 2829
Eco32I GATATC 1 cut(s) 2897
Eco47I GGWCC 3 cut(s) 2065, 2188, 3107
Eco53kI GAGCTC 3 cut(s) 36, 2445, 2766
Eco57I CTGAAG 5 cut(s) 41, 440, 1686, 1806, 4254
Eco88I CYCGRG 2 cut(s) 48, 4163
EcoICRI GAGCTC 3 cut(s) 36, 2445, 2766
EcoO109I RGGNCCY 1 cut(s) 352
EcoRI GAATTC 1 cut(s) 2543
EcoRII CCWGG 7 cut(s) 679, 724, 854, 1055, 1267, 1499, 2067
EcoRV GATATC 1 cut(s) 2897
EcoT14I CCWWGG 6 cut(s) 285, 447, 2508, 3100, 3948, 3975
EcoT22I ATGCAT 3 cut(s) 600, 1822, 3946
EcoT38I GRGCYC 4 cut(s) 38, 236, 2447, 2768
ErhI CCWWGG 6 cut(s) 285, 447, 2508, 3100, 3948, 3975
FalI AAGNNNNNCTT 8 cut(s) 413, 445, 1044, 1076, 3279, 3311, 4234, 4266
FaqI GGGAC 3 cut(s) 629, 717, 1444
FauI CCCGC 3 cut(s) 182, 243, 636
FauNDI CATATG 1 cut(s) 1750
FbaI TGATCA 3 cut(s) 481, 2428, 3337
FriOI GRGCYC 4 cut(s) 38, 236, 2447, 2768
FspBI CTAG 7 cut(s) 339, 1529, 1730, 1982, 2696, 3617, 3702
GlaI GCGC 6 cut(s) 1186, 1536, 2033, 2238, 4227, 4240
GsaI CCCAGC 3 cut(s) 1299, 1728, 4220
GsuI CTGGAG 5 cut(s) 390, 708, 1290, 2431, 3687
HaeII RGCGCY 3 cut(s) 1188, 2240, 4229
HapII CCGG 3 cut(s) 294, 1938, 3763
HgaI GACGC 2 cut(s) 496, 1608
HhaI GCGC 6 cut(s) 1187, 1537, 2034, 2239, 4228, 4241
Hin1I GRCGYC 1 cut(s) 396
Hin6I GCGC 6 cut(s) 1185, 1535, 2032, 2237, 4226, 4239
HinP1I GCGC 6 cut(s) 1185, 1535, 2032, 2237, 4226, 4239
HindIII AAGCTT 3 cut(s) 615, 1788, 2277
HpaII CCGG 3 cut(s) 294, 1938, 3763
HphI GGTGA 5 cut(s) 2141, 2370, 2768, 3806, 3920
Hpy166II GTNNAC 4 cut(s) 280, 1646, 2576, 4374
Hpy8I GTNNAC 4 cut(s) 280, 1646, 2576, 4374
Hpy99I CGWCG 3 cut(s) 101, 398, 3690
HpyCH4IV ACGT 4 cut(s) 396, 544, 3605, 3688
HpySE526I ACGT 4 cut(s) 396, 544, 3605, 3688
Hsp92I GRCGYC 1 cut(s) 396
HspAI GCGC 6 cut(s) 1185, 1535, 2032, 2237, 4226, 4239
KpnI GGTACC 1 cut(s) 29
Ksp22I TGATCA 3 cut(s) 481, 2428, 3337
LguI GCTCTTC 2 cut(s) 883, 1599
Lsp1109I GCAGC 9 cut(s) 259, 1225, 1972, 2677, 2696, 2718, 3527, 3802, 4321
MaeI CTAG 7 cut(s) 339, 1529, 1730, 1982, 2696, 3617, 3702
MaeII ACGT 4 cut(s) 396, 544, 3605, 3688
MaeIII GTNAC 8 cut(s) 322, 896, 959, 1020, 1030, 1654, 1780, 2153
MbiI CCGCTC 2 cut(s) 161, 241
MfeI CAATTG 2 cut(s) 462, 4349
MflI RGATCY 4 cut(s) 426, 1170, 1264, 2437
MhlI GDGCHC 7 cut(s) 38, 193, 236, 282, 1453, 2447, 2768
MlsI TGGCCA 1 cut(s) 1094
MluNI TGGCCA 1 cut(s) 1094
MlyI GAGTC 5 cut(s) 881, 1426, 2234, 3794, 4130
MmeI TCCRAC 6 cut(s) 236, 2198, 2506, 3949, 4065, 4302
Mox20I TGGCCA 1 cut(s) 1094
Mph1103I ATGCAT 3 cut(s) 600, 1822, 3946
MroXI GAANNNNTTC 5 cut(s) 1411, 2169, 2366, 2593, 2881
MscI TGGCCA 1 cut(s) 1094
MslI CAYNNNNRTG 3 cut(s) 714, 1203, 4074
Msp20I TGGCCA 1 cut(s) 1094
MspA1I CMGCKG 2 cut(s) 410, 2709
MspCI CTTAAG 1 cut(s) 3500
MspI CCGG 3 cut(s) 294, 1938, 3763
MspR9I CCNGG 8 cut(s) 681, 726, 856, 1057, 1269, 1501, 2069, 3764
MunI CAATTG 2 cut(s) 462, 4349
Mva1269I GAATGC 4 cut(s) 210, 598, 3320, 3627
MvaI CCWGG 7 cut(s) 681, 726, 856, 1057, 1269, 1501, 2069
MvnI CGCG 1 cut(s) 4239
NciI CCSGG 1 cut(s) 3764
NcoI CCATGG 1 cut(s) 3975
NdeI CATATG 1 cut(s) 1750
NheI GCTAGC 1 cut(s) 1981
NlaIV GGNNCC 5 cut(s) 27, 115, 1241, 1476, 3024
NmuCI GTSAC 3 cut(s) 959, 1020, 2153
NsiI ATGCAT 3 cut(s) 600, 1822, 3946
NspI RCATGY 8 cut(s) 949, 1946, 2127, 2304, 2807, 2969, 3967, 4175
PaeI GCATGC 1 cut(s) 949
PaeR7I CTCGAG 1 cut(s) 48
PagI TCATGA 1 cut(s) 1105
PciI ACATGT 2 cut(s) 1942, 2965
PciSI GCTCTTC 2 cut(s) 883, 1599
PctI GAATGC 4 cut(s) 210, 598, 3320, 3627
PdmI GAANNNNTTC 5 cut(s) 1411, 2169, 2366, 2593, 2881
PflFI GACNNNGTC 1 cut(s) 1067
PflMI CCANNNNNTGG 3 cut(s) 2514, 3466, 4182
PfoI TCCNGGA 3 cut(s) 854, 1267, 3762
PleI GAGTC 5 cut(s) 880, 1426, 2233, 3793, 4130
PpsI GAGTC 5 cut(s) 880, 1426, 2233, 3793, 4130
Ppu21I YACGTR 1 cut(s) 3606
PscI ACATGT 2 cut(s) 1942, 2965
PshAI GACNNNNGTC 1 cut(s) 1758
Psp124BI GAGCTC 3 cut(s) 38, 2447, 2768
Psp6I CCWGG 7 cut(s) 679, 724, 854, 1055, 1267, 1499, 2067
PspFI CCCAGC 3 cut(s) 1295, 1724, 4216
PspGI CCWGG 7 cut(s) 679, 724, 854, 1055, 1267, 1499, 2067
PspN4I GGNNCC 5 cut(s) 27, 115, 1241, 1476, 3024
PspPI GGNCC 8 cut(s) 352, 506, 2065, 2188, 2505, 3023, 3107, 3989
PstI CTGCAG 4 cut(s) 1915, 2684, 3115, 3205
PstNI CAGNNNCTG 1 cut(s) 1880
PsuI RGATCY 4 cut(s) 426, 1170, 1264, 2437
PsyI GACNNNGTC 1 cut(s) 1067
PvuII CAGCTG 2 cut(s) 410, 2709
RsaI GTAC 7 cut(s) 27, 1884, 2931, 2944, 3098, 4013, 4170
RsaNI GTAC 7 cut(s) 26, 1883, 2930, 2943, 3097, 4012, 4169
RseI CAYNNNNRTG 3 cut(s) 714, 1203, 4074
SacI GAGCTC 3 cut(s) 38, 2447, 2768
SapI GCTCTTC 2 cut(s) 883, 1599
Sau96I GGNCC 8 cut(s) 352, 506, 2065, 2188, 2505, 3023, 3107, 3989
SchI GAGTC 5 cut(s) 881, 1426, 2234, 3794, 4130
ScrFI CCNGG 8 cut(s) 681, 726, 856, 1057, 1269, 1501, 2069, 3764
SduI GDGCHC 7 cut(s) 38, 193, 236, 282, 1453, 2447, 2768
SfcI CTRYAG 5 cut(s) 1635, 1911, 2680, 3111, 3201
Sfr274I CTCGAG 1 cut(s) 48
SinI GGWCC 3 cut(s) 2065, 2188, 3107
SlaI CTCGAG 1 cut(s) 48
SmiMI CAYNNNNRTG 3 cut(s) 714, 1203, 4074
SmlI CTYRAG 7 cut(s) 48, 227, 1372, 2004, 2648, 3425, 3500
SmoI CTYRAG 7 cut(s) 48, 227, 1372, 2004, 2648, 3425, 3500
SnaBI TACGTA 1 cut(s) 3606
SphI GCATGC 1 cut(s) 949
SspI AATATT 1 cut(s) 2594
SspMI CTAG 7 cut(s) 339, 1529, 1730, 1982, 2696, 3617, 3702
SstI GAGCTC 3 cut(s) 38, 2447, 2768
StyD4I CCNGG 8 cut(s) 679, 724, 854, 1055, 1267, 1499, 2067, 3762
StyI CCWWGG 6 cut(s) 285, 447, 2508, 3100, 3948, 3975
TaiI ACGT 4 cut(s) 399, 547, 3608, 3691
TaqII GACCGA 1 cut(s) 236
TatI WGTACW 3 cut(s) 2929, 2942, 4011
TauI GCSGC 3 cut(s) 114, 241, 293
TscAI CASTG 8 cut(s) 65, 189, 325, 1476, 2526, 2737, 2746, 3777
TseFI GTSAC 3 cut(s) 959, 1020, 2153
TseI GCWGC 9 cut(s) 272, 1213, 1985, 2665, 2706, 2709, 3515, 3815, 4309
Tsp45I GTSAC 3 cut(s) 959, 1020, 2153
TspGWI ACGGA 2 cut(s) 1219, 1878
TspRI CASTG 8 cut(s) 65, 189, 325, 1476, 2526, 2737, 2746, 3777
Tth111I GACNNNGTC 1 cut(s) 1067
Van91I CCANNNNNTGG 3 cut(s) 2514, 3466, 4182
Vha464I CTTAAG 1 cut(s) 3500
VneI GTGCAC 1 cut(s) 278
VpaK11BI GGWCC 3 cut(s) 2065, 2188, 3107
XapI RAATTY 9 cut(s) 586, 1523, 1888, 2345, 2387, 2543, 2773, 3866, 4319
XbaI TCTAGA 1 cut(s) 1528
XceI RCATGY 8 cut(s) 949, 1946, 2127, 2304, 2807, 2969, 3967, 4175
XhoI CTCGAG 1 cut(s) 48
XmnI GAANNNNTTC 5 cut(s) 1411, 2169, 2366, 2593, 2881
XspI CTAG 7 cut(s) 339, 1529, 1730, 1982, 2696, 3617, 3702
ZraI GACGTC 1 cut(s) 397
Zsp2I ATGCAT 3 cut(s) 600, 1822, 3946
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.