Rroxscaffold_173G00434950

transcription, DNA-templated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000173
OG0011317
Physical Location & Seq
Forward (+)
2583 .. 10133
7551 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_173G00434950.1

Sequence Viewer

Length: 3453 bp
ATGCACGAGTCTGAAAAACATAAACGAGGTGGTAACGATGAGTTTTTACGGGTATTGTTTTGGCAATTTCACAATTTTCGCATGCTACTTGGAAGTGACTTGCTTCTGTTCAGTAATGAAAAATATGTTGCTGTTAGCCTGCATCTATGGGACGTTTCCCGAGAGGTTAAACCTATAACTTGGCTTGAAGCCTGGCTAGACAATGTCATGGCTAGTGTGCCAGAGATGGCCATCTGTTATCATAAAAATGGTGTGGTCCATAGTTACGAGCTTCTGAAAACAGATGATATATTTCTACTGAAAGGAATATCTGATGATGGCACTCCTGCTTTTCATCCTTATGTGGTACAGCAAAATGGCCTTGCTGTTTTAAGGTTCCTTCAAGAAAACTGCAAGCAAGATCCTGGAGCTTATTGGCTGTATAAAAGTGCTGGGGAAAATGACATTCAACTTTTCGATCTTTCTGTTATTCCAAAGAACCACTCTTCTAATGATAGCGATGATAGCTCAAGCTCTCTGTCTTCTTTATTGCATCGACGGAGAAGTGATTCCTTGTATTCATTGGGGACTCTCTTGTATCGAAGTGCTCACAGGCTCTCACTTGCAATGGCTCCAAACAATGTGGCAAAGTGTGCGAGGTTCTTTAGAAAATGTTTGGAACTTCTTGATGAGCCAAATCATCTGGTTGTACGTGCATCTGCCCATGAACAATTTGCTAGGCTTATTTTGAACAATGATGATGAGCTGGAATTAACGTCTGATGCTCTGCCAGCAGAATGTGAGCTTACAGTTAGCCATGCTCAAGAAGAGTCTATAGACTTTTTAAGTAGTATTTCTGAATCGTTAGTCCATGATCAGTTATTATCTCTAGTGGGAGAGGAAAAGTCATGTGAAGATGGACCAAGTTTTCAAGATATAGTACCAGGTGCTTCTGTAAATATGACTCTAGAAGCTAATGCACATTCTCCAAGAAAATTGATAACAGCAGGTGACATGGACTTTGGAGATTTGCCAGAACCCATACCAAATTTCTCTTGTGATGAAAGATCTACAGTTTGCAAATTGTCTCCAACAACAACAGACGTAGTTCAGAGTATTGCTGAAACCATCTCCTCAAAACTTGCTGCAATACACCATGTTTCTCAAGCTATTAAATCTCTTAGGTTGATGCGGCAACTGCATATTACAGAACCCAAGTTAGTGGGCCAGGAATCCCAGGATAGTGAAACTCAAGTTGAAAGTAGTAGGTCAGCTTCATCTGTTAATCTTTCTGTTTGTGCATGTGGTGATGTTGACTGTATCGAAGTTTGTGACATTCGGGAATGGCTTCCCACATCAACGTTGGACCATAAGTTATGGAAGCTGGTTCTTTTGCTTGGGGAATCTTATTTGGCACTTGGACAAGCTTATTTAGAGGATGACCAGTTACATCAAGCTTTGAAGGTTGTAGAATTAGCATGCTCTGTCTATGGATCAATGCCTCAGCATCTTGAAGATACAAAATTCATTTCTTCAATGAATAGTTGCTGGTCTTCAAAGTCAAAAAATAATAAAACAAATAAAAAGACGAGATCATATAATAGTGATCAGAGATTTCTAAAGAGCTCAAATGGTGATTGTCTGACTTTTGAGCAATTCTCATCCATTTACCTCTTCTGGGCCAAGGCATGGATATTAGTGGGAGATGTGTATGTTGAGTTCCATAAAGCTAAGGATAGTATTATATCTGAACCGGAAGAGAGAAAACATTCTACTTCAGAGGTAAAGGTATCATCTGAGGTTGTGAAAGAAGTTAAAAGGCTTAAGAGGAAGCTGGGGCAGCATGTGCAGAACTGCACTTCATGTTCTTTAGTAAATTGCAGCTGTCAAAGTGATAGGGCAAGCAGTGGTAGCAGTGCTAGTAGCAGTAGTGGTCCAAGTGGTGATATGCGCTCGGTAAGTTATGGCAGGAAATACATTAAAAGACCATATCCTAAGAGCAACACCTACCCACATTTGAGAGACCTGGAAGATAGTCTTTGTTTGGAAGCGGAGAATAAAAACATACTGGATGTTGAGTCGACAGTGGAGAATAAAAACATACTGGATGTTGAGTCTACAGTGGTGTCTCAGTGCGATGTTGCTTTGAAAAAAACTGGGGGAATATTCAAGTACCTTGGAGGTCCTGCAATTGGAGATGTTGAATACAATCTTTCAGCAGCCCTATGCTGTTATGAAGAAGCTAGAAAGGCATTAGGTGGTTTTCCATCTAGTTCAGAAGAGCTACAATCTGTAATGAAAAAGAAAGGGTGGGTATGCAATGAATTGGGCCGCAATAGACTTGCGAGGAAAGAACTGAGCAAAGCTGAATCTGCTTTTATAGATGCTATAAAATCATTCAGAGAAGTTTCTGATCATACCAACATTGTACTAATAAACTGCAACTTGGGCCATGGAAGACGAGCGGTGGCAGAAGAGGTGGTATCAAAGATTGATGGTCTTAAGATGCATTCCACATATCCTAATGCTTATGTTCATGTACTGGAGACTGCAAAATTGGAATATAGCGAATCACTTAAATATTACGGGGCAGCAAAGGCAGAGCTGAGTGCTTTTGTTGAAGACTCTGGCTCTGTGTTGAATGATTTGAGGACTGAGGTATGCACACAGTTCGCTCATACTTATCTGAGACTCGGAATGCTATTGGCAAGAGAAGATAGTACAGTGGAAGTTTATGGAACTGGAGTGTTGGAAGACATGCATACAGGGTCCACTAGTCCTACTGGAAGAAAATCAAGAAAAGAATCACGAAAGCATGAGATTTCAGCAAATGATGCCATTAGGAAGGCACTGTCTGTTTATGAATCATTAGGTGAACTACGGAAACAGGAGGCGGCATATGCTTATTATCAGCTCGCTTGCTACCAAAGGGATTGTTGTTTGAAGTTTTTGGAGCCAGATCAAAAGAAATGCAACTTATCCATTGGTGAAAACAGTATTCTTCAGCGGGTCAAGCAGTATGCATCCTTGGCAGAGAGGAACTGGCAAAAGGCCATGGGTTTCTATGGCCCAAAAACACATCCTACAATGTACCTAACTATTCTTATAGAGAGATCAGAGCTCTCATTGAAAGTTTCAAGCCACTTGCACTCAAATGTGATGCTGGAATCAGCTGTGTCTTGTTTGCTTGAAGGACGGTATGTATCGTCGGAAACAGATTCAGACTCTTTGAAGAGTGGTGACTCTGAGGTACATGCCAAATTTTGGAATCAGTTGCAGATGGTTTTGAAGAAGATGTTGGCCGTGACACTTTCAGCTAGTGGCAACAAATTACCTGTTTCTCAACCTCGGCAACTGCAGACTATATCCAACAGATCTGGGGATGCTGAAAAGCTCAGGGAGCTTTACAAAATATCTCTCAAGTGCACGGAGTTGAGTCAGTTAGGTGCTATGCACAACTTGTGGACCTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1150

Amino Acids

128.54

Weight (kDa)

6.04

Isoelectric Point (pI)

50.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDRF1_N PF23788 6 - 260 5.6e-35 EDRF1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 977
Acc36I ACCTGC 1 cut(s) 977
AccB7I CCANNNNNTGG 2 cut(s) 1668, 3243
AccBSI CCGCTC 1 cut(s) 2444
AccI GTMKAC 2 cut(s) 2060, 2096
AciI CCGC 6 cut(s) 1171, 2030, 2311, 2444, 2873, 2986
AclI AACGTT 1 cut(s) 1340
AclWI GGATC 2 cut(s) 395, 1480
AcoI YGGCCR 2 cut(s) 228, 3279
AcsI RAATTY 3 cut(s) 1027, 1502, 3239
AcuI CTGAAG 2 cut(s) 1740, 2966
AfaI GTAC 9 cut(s) 348, 690, 921, 2153, 2409, 2520, 2701, 3071, 3231
AfiI CCNNNNNNNGG 4 cut(s) 1657, 1668, 2171, 3243
AflII CTTAAG 2 cut(s) 1802, 2480
AhlI ACTAGT 1 cut(s) 2753
AjnI CCWGG 6 cut(s) 191, 403, 922, 1206, 1215, 2004
AjuI GAANNNNNNNTTGG 2 cut(s) 3260, 3292
Alw21I GWGCWC 4 cut(s) 589, 1607, 3102, 3407
Alw26I GTCTC 5 cut(s) 1071, 1995, 2112, 2519, 2662
Alw44I GTGCAC 1 cut(s) 3403
AlwI GGATC 2 cut(s) 395, 1480
Ama87I CYCGRG 1 cut(s) 159
AoxI GGCC 9 cut(s) 228, 358, 1204, 1659, 2308, 2428, 3030, 3046, 3279
ApaLI GTGCAC 1 cut(s) 3403
ApeKI GCWGC 5 cut(s) 1124, 1819, 1860, 2198, 2570
ApoI RAATTY 3 cut(s) 1027, 1502, 3239
ArsI GACNNNNNNTTYG 2 cut(s) 983, 1015
Asp700I GAANNNNTTC 3 cut(s) 547, 1326, 1747
AspLEI GCGC 1 cut(s) 1932
AsuHPI GGTGA 7 cut(s) 1001, 1298, 1625, 1934, 2864, 2978, 3230
AvaI CYCGRG 1 cut(s) 159
AvaII GGWCC 7 cut(s) 256, 899, 1345, 1913, 2162, 2748, 3444
BaeGI GKGCMC 1 cut(s) 3407
BalI TGGCCA 1 cut(s) 230
BanII GRGCYC 2 cut(s) 1607, 3102
BarI GAAGNNNNNNTAC 2 cut(s) 1237, 1269
BauI CACGAG 1 cut(s) 5
BbsI GAAGAC 5 cut(s) 513, 1524, 2443, 2607, 2739
Bbv12I GWGCWC 4 cut(s) 589, 1607, 3102, 3407
BbvCI CCTCAGC 1 cut(s) 1482
BbvI GCAGC 5 cut(s) 1111, 1831, 1872, 2210, 2582
BccI CCATC 8 cut(s) 220, 239, 311, 890, 1115, 2254, 2468, 3253
BceAI ACGGC 1 cut(s) 3266
BcgI CGANNNNNNTGC 2 cut(s) 2632, 2666
BciT130I CCWGG 6 cut(s) 193, 405, 924, 1208, 1217, 2006
BclI TGATCA 3 cut(s) 853, 1585, 2392
BcoDI GTCTC 5 cut(s) 1071, 1995, 2112, 2519, 2662
BcuI ACTAGT 1 cut(s) 2753
BfmI CTRYAG 4 cut(s) 813, 1050, 2097, 3335
BfrI CTTAAG 2 cut(s) 1802, 2480
BfuAI ACCTGC 1 cut(s) 977
BglII AGATCT 2 cut(s) 1046, 3353
BisI GCNGC 8 cut(s) 1125, 1172, 1820, 1861, 2199, 2311, 2571, 2874
BlsI GCNGC 8 cut(s) 1126, 1173, 1821, 1862, 2200, 2312, 2572, 2875
Bme1390I CCNGG 6 cut(s) 193, 405, 924, 1208, 1217, 2006
Bme18I GGWCC 7 cut(s) 256, 899, 1345, 1913, 2162, 2748, 3444
BmeT110I CYCGRG 1 cut(s) 159
BmiI GGNNCC 4 cut(s) 377, 612, 2749, 2934
BmrFI CCNGG 6 cut(s) 193, 405, 924, 1208, 1217, 2006
BmrI ACTGGG 1 cut(s) 2145
BmuI ACTGGG 1 cut(s) 2145
BpiI GAAGAC 5 cut(s) 513, 1524, 2443, 2607, 2739
BplI GAGNNNNNCTC 2 cut(s) 1622, 1654
BpmI CTGGAG 3 cut(s) 426, 2543, 2742
Bpu10I CCTNAGC 3 cut(s) 1482, 1710, 3374
BpuEI CTTGAG 5 cut(s) 493, 786, 1128, 1215, 3383
BsaAI YACGTR 1 cut(s) 692
BsaBI GATNNNNATC 1 cut(s) 230
BsaI GGTCTC 1 cut(s) 1995
BsaJI CCNNGG 7 cut(s) 1215, 1662, 2155, 2431, 3006, 3033, 3326
BsaWI WCCGGW 1 cut(s) 1732
Bsc4I CCNNNNNNNGG 4 cut(s) 1657, 1668, 2171, 3243
Bse1I ACTGG 8 cut(s) 1423, 2052, 2088, 2140, 2526, 2725, 2767, 3026
Bse3DI GCAATG 2 cut(s) 612, 2305
Bse8I GATNNNNATC 1 cut(s) 230
BseBI CCWGG 6 cut(s) 193, 405, 924, 1208, 1217, 2006
BseDI CCNNGG 7 cut(s) 1215, 1662, 2155, 2431, 3006, 3033, 3326
BseGI GGATG 8 cut(s) 334, 1423, 1640, 2056, 2092, 3002, 3058, 3367
BseJI GATNNNNATC 1 cut(s) 230
BseLI CCNNNNNNNGG 4 cut(s) 1657, 1668, 2171, 3243
BseMI GCAATG 2 cut(s) 612, 2305
BseMII CTCAG 9 cut(s) 1496, 1767, 2123, 2327, 2576, 2625, 2657, 3216, 3388
BseNI ACTGG 8 cut(s) 1423, 2052, 2088, 2140, 2526, 2725, 2767, 3026
BseRI GAGGAG 1 cut(s) 1102
BseSI GKGCMC 1 cut(s) 3407
BseXI GCAGC 5 cut(s) 1111, 1831, 1872, 2210, 2582
BseYI CCCAGC 2 cut(s) 431, 1813
BsgI GTGCAG 2 cut(s) 1819, 1847
BshFI GGCC 9 cut(s) 230, 360, 1206, 1661, 2310, 2430, 3032, 3048, 3281
BsiHKAI GWGCWC 4 cut(s) 589, 1607, 3102, 3407
BsiHKCI CYCGRG 1 cut(s) 159
BsiSI CCGG 1 cut(s) 1733
BslFI GGGAC 2 cut(s) 164, 580
BslI CCNNNNNNNGG 4 cut(s) 1657, 1668, 2171, 3243
BsmAI GTCTC 5 cut(s) 1071, 1995, 2112, 2519, 2662
BsmFI GGGAC 2 cut(s) 164, 580
BsmI GAATGC 2 cut(s) 2488, 2682
BsnI GGCC 9 cut(s) 230, 360, 1206, 1661, 2310, 2430, 3032, 3048, 3281
Bso31I GGTCTC 1 cut(s) 1995
BsoBI CYCGRG 1 cut(s) 159
Bsp1286I GDGCHC 4 cut(s) 589, 1607, 3102, 3407
Bsp19I CCATGG 2 cut(s) 2431, 3033
BspACI CCGC 6 cut(s) 1171, 2030, 2311, 2444, 2873, 2986
BspANI GGCC 9 cut(s) 230, 360, 1206, 1661, 2310, 2430, 3032, 3048, 3281
BspCNI CTCAG 9 cut(s) 1495, 1768, 2122, 2328, 2577, 2626, 2658, 3217, 3387
BspLI GGNNCC 4 cut(s) 377, 612, 2749, 2934
BspMAI CTGCAG 1 cut(s) 3339
BspMI ACCTGC 1 cut(s) 977
BspPI GGATC 2 cut(s) 395, 1480
BspQI GCTCTTC 1 cut(s) 2253
BspTI CTTAAG 2 cut(s) 1802, 2480
BspTNI GGTCTC 1 cut(s) 1995
BsrBI CCGCTC 1 cut(s) 2444
BsrDI GCAATG 2 cut(s) 612, 2305
BsrI ACTGG 8 cut(s) 1423, 2052, 2088, 2140, 2526, 2725, 2767, 3026
BssECI CCNNGG 7 cut(s) 1215, 1662, 2155, 2431, 3006, 3033, 3326
BssSI CACGAG 1 cut(s) 5
BssT1I CCWWGG 5 cut(s) 1662, 2155, 2431, 3006, 3033
Bst2BI CACGAG 1 cut(s) 5
Bst2UI CCWGG 6 cut(s) 193, 405, 924, 1208, 1217, 2006
Bst6I CTCTTC 7 cut(s) 490, 801, 1658, 1731, 2253, 2448, 3206
BstAFI CTTAAG 2 cut(s) 1802, 2480
BstAPI GCANNNNNTGC 3 cut(s) 632, 1825, 2813
BstBAI YACGTR 1 cut(s) 692
BstC8I GCNNGC 8 cut(s) 83, 140, 395, 771, 1459, 1882, 2895, 2899
BstDSI CCRYGG 2 cut(s) 2431, 3033
BstF5I GGATG 8 cut(s) 334, 1423, 1640, 2056, 2092, 3002, 3058, 3367
BstHHI GCGC 1 cut(s) 1932
BstMAI GTCTC 5 cut(s) 1071, 1995, 2112, 2519, 2662
BstNI CCWGG 6 cut(s) 193, 405, 924, 1208, 1217, 2006
BstNSI RCATGY 6 cut(s) 85, 1284, 1461, 1826, 2740, 3236
BstSCI CCNGG 6 cut(s) 191, 403, 922, 1206, 1215, 2004
BstSFI CTRYAG 4 cut(s) 813, 1050, 2097, 3335
BstSLI GKGCMC 1 cut(s) 3407
BstV1I GCAGC 5 cut(s) 1111, 1831, 1872, 2210, 2582
BstV2I GAAGAC 5 cut(s) 513, 1524, 2443, 2607, 2739
BstX2I RGATCY 3 cut(s) 400, 1046, 3353
BstXI CCANNNNNNTGG 1 cut(s) 1201
BstYI RGATCY 3 cut(s) 400, 1046, 3353
BsuRI GGCC 9 cut(s) 230, 360, 1206, 1661, 2310, 2430, 3032, 3048, 3281
BtgI CCRYGG 2 cut(s) 2431, 3033
BtgZI GCGATG 2 cut(s) 513, 2130
BtsCI GGATG 8 cut(s) 334, 1423, 1640, 2056, 2092, 3002, 3058, 3367
BtsI GCAGTG 2 cut(s) 1891, 1900
BtsIMutI CAGTG 7 cut(s) 1891, 1900, 2070, 2106, 2117, 2709, 2828
BveI ACCTGC 1 cut(s) 977
Cac8I GCNNGC 8 cut(s) 83, 140, 395, 771, 1459, 1882, 2895, 2899
CfoI GCGC 1 cut(s) 1932
CsiI ACCWGGT 1 cut(s) 922
Csp6I GTAC 9 cut(s) 347, 689, 920, 2152, 2408, 2519, 2700, 3070, 3230
CspCI CAANNNNNGTGG 2 cut(s) 603, 638
CviQI GTAC 9 cut(s) 347, 689, 920, 2152, 2408, 2519, 2700, 3070, 3230
EaeI YGGCCR 2 cut(s) 228, 3279
Eam1104I CTCTTC 7 cut(s) 490, 801, 1658, 1731, 2253, 2448, 3206
EarI CTCTTC 7 cut(s) 490, 801, 1658, 1731, 2253, 2448, 3206
Ecl136II GAGCTC 2 cut(s) 1605, 3100
Eco130I CCWWGG 5 cut(s) 1662, 2155, 2431, 3006, 3033
Eco24I GRGCYC 2 cut(s) 1607, 3102
Eco31I GGTCTC 1 cut(s) 1995
Eco47I GGWCC 7 cut(s) 256, 899, 1345, 1913, 2162, 2748, 3444
Eco53kI GAGCTC 2 cut(s) 1605, 3100
Eco57I CTGAAG 2 cut(s) 1740, 2966
Eco88I CYCGRG 1 cut(s) 159
EcoICRI GAGCTC 2 cut(s) 1605, 3100
EcoO109I RGGNCCY 1 cut(s) 2162
EcoRII CCWGG 6 cut(s) 191, 403, 922, 1206, 1215, 2004
EcoT14I CCWWGG 5 cut(s) 1662, 2155, 2431, 3006, 3033
EcoT22I ATGCAT 3 cut(s) 2490, 2742, 3004
EcoT38I GRGCYC 2 cut(s) 1607, 3102
ErhI CCWWGG 5 cut(s) 1662, 2155, 2431, 3006, 3033
FalI AAGNNNNNCTT 2 cut(s) 2001, 2033
FaqI GGGAC 2 cut(s) 164, 580
FauI CCCGC 1 cut(s) 2979
FauNDI CATATG 1 cut(s) 2878
FbaI TGATCA 3 cut(s) 853, 1585, 2392
FblI GTMKAC 2 cut(s) 2060, 2096
Fnu4HI GCNGC 8 cut(s) 1125, 1172, 1820, 1861, 2199, 2311, 2571, 2874
FokI GGATG 8 cut(s) 321, 1430, 1627, 2063, 2099, 2989, 3045, 3374
FriOI GRGCYC 2 cut(s) 1607, 3102
Fsp4HI GCNGC 8 cut(s) 1125, 1172, 1820, 1861, 2199, 2311, 2571, 2874
GlaI GCGC 1 cut(s) 1931
GluI GCNGC 8 cut(s) 1125, 1172, 1820, 1861, 2199, 2311, 2571, 2874
GsaI CCCAGC 2 cut(s) 435, 1817
GsuI CTGGAG 3 cut(s) 426, 2543, 2742
HaeIII GGCC 9 cut(s) 230, 360, 1206, 1661, 2310, 2430, 3032, 3048, 3281
HapII CCGG 1 cut(s) 1733
HhaI GCGC 1 cut(s) 1932
Hin6I GCGC 1 cut(s) 1930
HinP1I GCGC 1 cut(s) 1930
HincII GTYRAC 2 cut(s) 1294, 2061
HindII GTYRAC 2 cut(s) 1294, 2061
HindIII AAGCTT 2 cut(s) 1404, 1434
HpaII CCGG 1 cut(s) 1733
HphI GGTGA 7 cut(s) 1001, 1298, 1625, 1934, 2864, 2978, 3230
Hpy166II GTNNAC 7 cut(s) 1294, 2061, 2097, 2751, 2855, 3405, 3444
Hpy8I GTNNAC 7 cut(s) 1294, 2061, 2097, 2751, 2855, 3405, 3444
Hpy99I CGWCG 2 cut(s) 540, 3190
HpyAV CCTTC 4 cut(s) 389, 1435, 2818, 3164
HpyCH4IV ACGT 5 cut(s) 153, 691, 755, 1083, 1340
HpySE526I ACGT 5 cut(s) 153, 691, 755, 1083, 1340
HspAI GCGC 1 cut(s) 1930
Ksp22I TGATCA 3 cut(s) 853, 1585, 2392
LguI GCTCTTC 1 cut(s) 2253
LmnI GCTCC 4 cut(s) 407, 616, 2932, 3379
Lsp1109I GCAGC 5 cut(s) 1111, 1831, 1872, 2210, 2582
MabI ACCWGGT 1 cut(s) 922
MaeII ACGT 5 cut(s) 153, 691, 755, 1083, 1340
MaeIII GTNAC 8 cut(s) 32, 95, 263, 989, 1310, 1425, 3218, 3283
MbiI CCGCTC 1 cut(s) 2444
MfeI CAATTG 1 cut(s) 2169
MflI RGATCY 3 cut(s) 400, 1046, 3353
MhlI GDGCHC 4 cut(s) 589, 1607, 3102, 3407
MlsI TGGCCA 1 cut(s) 230
MluNI TGGCCA 1 cut(s) 230
MmeI TCCRAC 5 cut(s) 1094, 1323, 2709, 3168, 3372
Mox20I TGGCCA 1 cut(s) 230
Mph1103I ATGCAT 3 cut(s) 2490, 2742, 3004
MroXI GAANNNNTTC 3 cut(s) 547, 1326, 1747
MscI TGGCCA 1 cut(s) 230
MslI CAYNNNNRTG 3 cut(s) 246, 339, 3132
Msp20I TGGCCA 1 cut(s) 230
MspA1I CMGCKG 3 cut(s) 1863, 2986, 3152
MspCI CTTAAG 2 cut(s) 1802, 2480
MspI CCGG 1 cut(s) 1733
MspR9I CCNGG 6 cut(s) 193, 405, 924, 1208, 1217, 2006
MunI CAATTG 1 cut(s) 2169
Mva1269I GAATGC 2 cut(s) 2488, 2682
MvaI CCWGG 6 cut(s) 193, 405, 924, 1208, 1217, 2006
NcoI CCATGG 2 cut(s) 2431, 3033
NdeI CATATG 1 cut(s) 2878
NlaIV GGNNCC 4 cut(s) 377, 612, 2749, 2934
NmeAIII GCCGAG 1 cut(s) 3307
NmuCI GTSAC 5 cut(s) 95, 989, 1310, 3218, 3283
NsiI ATGCAT 3 cut(s) 2490, 2742, 3004
NspI RCATGY 6 cut(s) 85, 1284, 1461, 1826, 2740, 3236
PaeI GCATGC 2 cut(s) 85, 1461
PaqCI CACCTGC 1 cut(s) 977
PciSI GCTCTTC 1 cut(s) 2253
PctI GAATGC 2 cut(s) 2488, 2682
PdmI GAANNNNTTC 3 cut(s) 547, 1326, 1747
PflFI GACNNNGTC 1 cut(s) 203
PflMI CCANNNNNTGG 2 cut(s) 1668, 3243
PfoI TCCNGGA 1 cut(s) 403
PkrI GCNGC 8 cut(s) 1126, 1173, 1821, 1862, 2200, 2312, 2572, 2875
Ppu21I YACGTR 1 cut(s) 692
PpuMI RGGWCCY 1 cut(s) 2162
Psp124BI GAGCTC 2 cut(s) 1607, 3102
Psp1406I AACGTT 1 cut(s) 1340
Psp5II RGGWCCY 1 cut(s) 2162
Psp6I CCWGG 6 cut(s) 191, 403, 922, 1206, 1215, 2004
PspFI CCCAGC 2 cut(s) 431, 1813
PspGI CCWGG 6 cut(s) 191, 403, 922, 1206, 1215, 2004
PspN4I GGNNCC 4 cut(s) 377, 612, 2749, 2934
PspPPI RGGWCCY 1 cut(s) 2162
PsrI GAACNNNNNNTAC 2 cut(s) 1682, 1714
PstI CTGCAG 1 cut(s) 3339
PsuI RGATCY 3 cut(s) 400, 1046, 3353
PsyI GACNNNGTC 1 cut(s) 203
PvuII CAGCTG 2 cut(s) 1863, 3152
RsaI GTAC 9 cut(s) 348, 690, 921, 2153, 2409, 2520, 2701, 3071, 3231
RsaNI GTAC 9 cut(s) 347, 689, 920, 2152, 2408, 2519, 2700, 3070, 3230
RseI CAYNNNNRTG 3 cut(s) 246, 339, 3132
SacI GAGCTC 2 cut(s) 1607, 3102
SalI GTCGAC 1 cut(s) 2059
SapI GCTCTTC 1 cut(s) 2253
SatI GCNGC 8 cut(s) 1125, 1172, 1820, 1861, 2199, 2311, 2571, 2874
ScrFI CCNGG 6 cut(s) 193, 405, 924, 1208, 1217, 2006
SduI GDGCHC 4 cut(s) 589, 1607, 3102, 3407
SexAI ACCWGGT 1 cut(s) 922
SfcI CTRYAG 4 cut(s) 813, 1050, 2097, 3335
SinI GGWCC 7 cut(s) 256, 899, 1345, 1913, 2162, 2748, 3444
SmiMI CAYNNNNRTG 3 cut(s) 246, 339, 3132
SmlI CTYRAG 7 cut(s) 508, 801, 1143, 1230, 1802, 2480, 3398
SmoI CTYRAG 7 cut(s) 508, 801, 1143, 1230, 1802, 2480, 3398
SpeI ACTAGT 1 cut(s) 2753
SphI GCATGC 2 cut(s) 85, 1461
SsiI CCGC 6 cut(s) 1171, 2030, 2311, 2444, 2873, 2986
SspI AATATT 2 cut(s) 2145, 2561
SstI GAGCTC 2 cut(s) 1607, 3102
StyD4I CCNGG 6 cut(s) 191, 403, 922, 1206, 1215, 2004
StyI CCWWGG 5 cut(s) 1662, 2155, 2431, 3006, 3033
TaiI ACGT 5 cut(s) 156, 694, 758, 1086, 1343
TaqI TCGA 5 cut(s) 456, 535, 580, 1302, 2060
TatI WGTACW 3 cut(s) 2407, 2518, 2699
TauI GCSGC 3 cut(s) 1174, 2313, 2876
TscAI CASTG 7 cut(s) 1891, 1900, 2070, 2106, 2117, 2709, 2835
TseFI GTSAC 5 cut(s) 95, 989, 1310, 3218, 3283
TseI GCWGC 5 cut(s) 1124, 1819, 1860, 2198, 2570
Tsp45I GTSAC 5 cut(s) 95, 989, 1310, 3218, 3283
TspGWI ACGGA 3 cut(s) 553, 2875, 3422
TspRI CASTG 7 cut(s) 1891, 1900, 2070, 2106, 2117, 2709, 2835
Tth111I GACNNNGTC 1 cut(s) 203
Van91I CCANNNNNTGG 2 cut(s) 1668, 3243
Vha464I CTTAAG 2 cut(s) 1802, 2480
VneI GTGCAC 1 cut(s) 3403
VpaK11BI GGWCC 7 cut(s) 256, 899, 1345, 1913, 2162, 2748, 3444
XapI RAATTY 3 cut(s) 1027, 1502, 3239
XbaI TCTAGA 1 cut(s) 946
XceI RCATGY 6 cut(s) 85, 1284, 1461, 1826, 2740, 3236
XcmI CCANNNNNNNNNTGG 1 cut(s) 1339
XmiI GTMKAC 2 cut(s) 2060, 2096
XmnI GAANNNNTTC 3 cut(s) 547, 1326, 1747
Zsp2I ATGCAT 3 cut(s) 2490, 2742, 3004
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.