Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0006478

Physical Location & Seq

Genomic Coordinates Forward (+)

15926676 .. 15935529

Gene Structure 8854 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G42470.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3783 bp

ATGCAGATTCTGTTTGTTGGTTATGGAGTGGAAGCAAAACAATCAGCAGGATATTGTGCTATGTATGATATCTGTGGAGCAAGAAGTGATGGGAAAGTACTAAATTGCCCTTTCAACATTCCTTCTGTGAAGCCTGATGATTTGTTATCTTCAAAGATTCAAAGCTTATGTCCAACAATCACTGGCAATGTTTGTTGCACAGAGACTCAATTTGATACATTACGTTCGCAAGTTCAACAGGCTATTCCTTTTATTGTTGGTTGTCCAGCATGTTTGAGGAACTTTCTGAATCTTTTTTGTGAACTTACTTGTTCTCCGGATCAGAGTTTGTTTATCAATGTAACTTCTACTACAAAGGTCAAGAATAATTCTACTGTAGATGGAATTCAGTACTATATAACTGATGATTTTGGAGCTGGGATGTATGAATCTTGCAAAAATGTAAAATTTGGTAGCTCGAATAGCCGAGCTTTAGACTTTCTCGGAGCCGGTGCAAAGAACTTTAAGGAGTGGTTTACATTTATTGGTCAGAAAGCGGGTGTCAATTTGCCAGGCTCACCGTATGGGATCGCTTTTTTGCCAACACCGCCTGTGTCGTCTGGAATGAGGCCTATGAATGTGTCTATTTATTCATGTGGTGATGAGTCTCTTGGCTGTTCTTGTGGTGATTGTCCTTCTGCAGCTACTTGCTCTAGTAAAGCAGAAGTGCCTACTCAGAAAAAACATTCTTGCTCTATCAAGATTGGATCACTTGAGGTGAAATGTGTTGATTTCATATTAGCCATTTTGTATATTGTATTGGTTTCATTGTTTCTTGGAGGAGGTTTACTTCACCCAGTAAGGGGTAAGAAAAAGACCTCACAGATGGGAACATTGTCAGAAGCTAGTGGAGAACGAAATTCTGTTAATCAGCAAAAACCAGACACTATTCAATCGCAGATGTTACAAAATACTCCACAGAGAAACTGGGGTCAGCTATCGACAGTACAAGGACACTTGGCCAACTTTTACGGGAAGTATGGGATTTGGGTAGCAAGACACCCCACTCTTGTTTTGTGTTTGTCAGTCTCTGTAGTTCTTCTCCTTTGTGTGGGTCTGATCCGGTTCAAAGTTGAGACACGGCCTGATAAGCTGTGGGTAGGATCAGGTAGCAGAGCTGCTGAAGAGAAACAATTCTTTGACACTCATCTTGCTCCTTTCTACAGAATTGAACAGCTAATAATAGCAACAGTTCAAACATCTTCTCACGAAAAGGCGCCAGAGATTTTGACAGATGACAATATTAAGTTACTTTTTGACATACAAAAGAAGGTCGATGGACTTCGGGCAAATCACTCAGGTTCAATGGTTTCTCTGACAGATATCTGCATGAAACCCCTGGGAGAGGATTGTGCCACACAGAGTGTTCTGCAGTATTTCAAAATGAAACCAGAAAATTATGACGACTATGGAGGCGTTGATCATGTTAAATATTGTTTTGAGCATTTCACCTCGACAGAATCATGTTTGAGTGCATTTAAGGGTCCACTTGATCCAACTACTGCACTGGGAGGTTTCTCAGGGAACAGTTTTAGTGAGGCTTCTGCTTTTCTTGTGACTTATCCTGTGGACAATTTTGTTGACAATAAAGGTAACAAAACTGAGAAAGCAGTAGCATGGGAGAAAGCCTTTATCCAACTGGCCAAGGATGAGTTGTTGCCAATGGTTCAAGCAAAAAATTTAACTCTTTCTTTCTCGTCGGAAAGTTCTATTGAAGAAGAACTGAAAAGAGAAAGCACAGCAGATGTCATAACTATAGCCATAAGTTATCTTGTCATGTTTGCCTATATATCACTCACACTTGGAGATTCACCTCGCTTAAAGTCCTTTTACATTACATCCAAGGTTCTGCTTGGTCTATCTGGTGTTCTACTTGTCATGCTTTCTGTCCTTGGCTCTGTAGGATTTTTCAGTGCCGTTGGGATGAAATCGACTCTAATCATAATGGAAGTTATACCTTTTCTTGTTTTGGCTGTCGGTGTTGATAATATGTGCATCCTGGTTCATGCTGTCAAGAGGCAAGAGCAAGAGCTGCCTTTGGAAAGAAGAATTAGCAATGCCCTTATGGAAGTTGGACCATCGATTACTCTAGCAAGTTTAGCCGAGATTTTAGCTTTCGCTGTTGGTGCTTTCATTAAAATGCCAGCTGTTCGAGTATTCTCCATGTTTGCTGCATTGGCTGTCCTTTTGGACTTTCTTCTCCAGATTACCGCGTTTGTAGCTTTGATAGTTTTTGACTTCCGACGGACGGAGGATAAGCGAGTTGATTGCTTCCCGTGTATTAAGACATCAAAATCATCTATTAGTGCTGAAAAAGGGGTTGGTCAAAGAAAAGCTGGCCTTTTGACTCGATATATGAAGGAAGTCCATGCACCTGTTCTTAGCCATTGGATAGTCAAAATAGTGGTCATTGCCTTCTTCTTTGGCTTAGCAATGGCTGGAATTGCATTGTCGACTCGTATTGAGCCTGGTTTGGAGCAACAGATTGTTCTTCCTCAAGACTCATATCTTCAGGGTTACTTCAACAATATTTCTACATATCTTCGAATCGGTCCACCTTTATACTTTGTTCTGAAGAATTATAACTACAGCTCAGAATCAAGACATACAAATCAACTTTGTTCCATTAACAAGTGTAATCCTAACTCTCTTTTAAATGAGATTGCAAGGGCTTCTTTGACTCCGGAACTAAGCTACATAGCTAAGCCAGCTGCTTCGTGGCTCGATGACTTTCTTGTGTGGTTATCTCCCGAGGCGTTTGGATGTTGCAGAAAGTTTACAAATGGTACCTTTTGCCCCCCTGATGACCAGCCTCCTTGTTGCCCTCCTGGTCAAACGAGCTGTGGCCTAAGTGAAGTATGCAAAGATTGTACCACGTGCTTCCGGCATGCAGATTTAAGTAGTGACCGCCCTTCCACAACACAATTCAAAGAGAAACTTCCTTGGTTCCTCAACGCGCTCCCTTCTGCTGATTGTGCTAAAGGTGGACATGGTGCTTATTCTAGTAGTGTTGATTTGCAAGGCTATGCAAATGGTATCATTCAAGCTTCATCGTTCCGTACTTATCACACACCCCTTAACAAGCAGGTTGACTTTGTTAATTCAATGAGAGCTGCTCAAGAATTTAGTGCAAAAGTTTCCCGTTCCTTAAAGATGGAGATATATCCTTATTCAGTGTTTTATATGTTCTTTGAGCAATATCTTGACATATGGAAAACTGCATTAATCAACCTCTCCATAGCCATCGCTGCCGTGTTTGTCGTGTGTTTAATCATCACATGCAGCTTTTGGAGCTCGGCAATTATTTTGCTGGTGATCGCGATGATCATTATTGATCTCCTGGGAGTTATGGCAGTCTTTCATATCCAGCTAAACGCACTATCGGTTGTAAATCTAATCATGTCCGTGGGCATAGCGGTTGAGTTTTGCGTACACATAACACACGCTTTCTCGATAAGCACTGGGGACAGAAACCATCGGATGAAAGAGGCGTTGGGTGGGATGGGAGCTTCAGTTTTCAGTGGAATTACATTGACGAAACTAGTTGGGGTGATTGTGCTCGGTTTCTCAAGATCAGAAGTCTTTGTGGTGTACTACTTCAAGATGTATTTGGCACTAGTCCTTCTCGGTTTCTTACACGGCCTTGTATTTTTACCGGTGTTCTTGAGCATGTTTGGTCCAGCTCCAAGACATGTAGAAGGGGAAAGGCAAGATCATCGACCTTCGGTTTCATCAGTACCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0003674 GO:0005488 GO:0005496 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005886 GO:0005887 GO:0006082 GO:0006464 GO:0006486 GO:0006629 GO:0006807 GO:0006810 GO:0006869 GO:0007034 GO:0007041 GO:0008150 GO:0008152 GO:0008202 GO:0008206 GO:0008289 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0010876 GO:0012505 GO:0015485 GO:0015850 GO:0015918 GO:0016020 GO:0016021 GO:0019538 GO:0019752 GO:0030301 GO:0031224 GO:0031226 GO:0031967 GO:0031975 GO:0032787 GO:0032934 GO:0033036 GO:0034645 GO:0036094 GO:0036211 GO:0042592 GO:0042632 GO:0043170 GO:0043178 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0048471 GO:0048878 GO:0051179 GO:0051234 GO:0055088 GO:0055092 GO:0065007 GO:0065008 GO:0070085 GO:0071702 GO:0071704 GO:0071944 GO:0097159 GO:1901135 GO:1901137 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901615

KEGG Pathways

Metabolic & Signaling

ko04142 ko04979 map04142 map04979

Pfam Domains

Protein Families

NPC1_N Patched

Protein Analysis

1260

Amino Acids

138.28

Weight (kDa)

7.72

Isoelectric Point (pI)

39.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NPC1_N	PF16414	17 - 246	2.4e-67	Niemann-Pick C1 N terminus
NPC1_MLD	PF22314	390 - 581	3.9e-37	NPC1, middle luminal domain
Patched	PF02460	603 - 757	1.6e-34	Patched SSD
Sterol-sensing	PF12349	625 - 775	1.8e-50	Sterol-sensing domain of SREBP cleavage-activation

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0006478)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G42470
fragaria_vesca	FvH4_5g25290 FvH4_5g25290 FvH4_5g25290 FvH4_5g25290 FvH4_5g25290 FvH4_5g25290 FvH4_5g25290
malus_domestica	MD06G1009400.v1.1
prunus_persica	Prupe.5G011100_v2.0.a1 Prupe.5G011100_v2.0.a1 Prupe.5G011100_v2.0.a1 Prupe.5G011100_v2.0.a1
pyrus_communis	pycom06g00780 pycom16g25890
rosa_chinensis	RchiOBHm_Chr7g0215501
rosa_laevigata	RLG00000002657
rosa_multiflora	Rmu_sc0005928.1_g000005
rosa_roxburghii	Rroxscaffold_3G00244190
rosa_rugosa	Rorug07G0154100 Rorug07G0154200
rosa_samantha	Rh7AG290300 Rh7BG282300 Rh7CG310300 Rh7DG294100
rosa_wichuraiana	Rw7G024790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2622
Acc36I	ACCTGC	1 cut(s)	3115
Acc65I	GGTACC	1 cut(s)	2825
AccB1I	GGYRCC	2 cut(s)	1255, 2825
AccI	GTMKAC	1 cut(s)	2492
AccII	CGCG	3 cut(s)	2252, 2996, 3359
AccIII	TCCGGA	2 cut(s)	316, 2722
AciI	CCGC	5 cut(s)	536, 587, 2250, 2947, 3455
AclWI	GGATC	6 cut(s)	327, 575, 754, 1093, 1150, 1526
AcoI	YGGCCR	2 cut(s)	999, 1680
AcsI	RAATTY	5 cut(s)	384, 446, 898, 1717, 3161
AcuI	CTGAAG	4 cut(s)	1182, 2534, 2633, 3534
AcvI	CACGTG	1 cut(s)	2916
AcyI	GRCGYC	1 cut(s)	1256
AdeI	CACNNNGTG	1 cut(s)	1403
AfaI	GTAC	9 cut(s)	99, 392, 987, 2827, 2911, 3100, 3471, 3632, 3777
AfiI	CCNNNNNNNGG	5 cut(s)	841, 842, 1090, 1384, 2287
AflIII	ACRYGT	1 cut(s)	3730
AgeI	ACCGGT	1 cut(s)	3694
AhlI	ACTAGT	2 cut(s)	3580, 3655
AjnI	CCWGG	6 cut(s)	550, 1377, 2037, 2506, 2866, 3378
AjuI	GAANNNNNNNTTGG	4 cut(s)	2343, 2375, 3515, 3547
Alw21I	GWGCWC	2 cut(s)	3335, 3600
Alw26I	GTCTC	4 cut(s)	197, 651, 1072, 1109
AlwI	GGATC	6 cut(s)	327, 575, 754, 1093, 1150, 1526
AlwNI	CAGNNNCTG	2 cut(s)	10, 1070
Ama87I	CYCGRG	1 cut(s)	2789
Aor13HI	TCCGGA	2 cut(s)	316, 2722
AoxI	GGCC	7 cut(s)	608, 999, 1121, 1680, 2377, 2884, 3679
ApeKI	GCWGC	8 cut(s)	680, 1157, 2071, 2210, 2750, 3152, 3287, 3321
ApoI	RAATTY	5 cut(s)	384, 446, 898, 1717, 3161
ArsI	GACNNNNNNTTYG	4 cut(s)	154, 186, 2430, 2462
AseI	ATTAAT	1 cut(s)	3263
AsiGI	ACCGGT	1 cut(s)	3694
Asp700I	GAANNNNTTC	1 cut(s)	1172
Asp718I	GGTACC	1 cut(s)	2825
AspLEI	GCGC	2 cut(s)	1258, 2998
AspS9I	GGNCC	4 cut(s)	1523, 2114, 2591, 3716
AsuHPI	GGTGA	9 cut(s)	549, 650, 677, 769, 824, 1480, 1842, 3364, 3601
AsuII	TTCGAA	1 cut(s)	2584
AvaI	CYCGRG	1 cut(s)	2789
AvaII	GGWCC	4 cut(s)	1523, 2114, 2591, 3716
BalI	TGGCCA	2 cut(s)	1001, 1682
BanI	GGYRCC	2 cut(s)	1255, 2825
BanII	GRGCYC	1 cut(s)	3335
BbrPI	CACGTG	1 cut(s)	2916
Bbv12I	GWGCWC	2 cut(s)	3335, 3600
BbvI	GCAGC	8 cut(s)	692, 1144, 2058, 2197, 2737, 3139, 3274, 3333
BccI	CCATC	9 cut(s)	83, 374, 859, 1310, 2125, 3187, 3290, 3522, 3535
BceAI	ACGGC	4 cut(s)	1136, 1940, 3275, 3694
BcgI	CGANNNNNNTGC	4 cut(s)	2289, 2323, 3737, 3771
BciT130I	CCWGG	6 cut(s)	552, 1379, 2039, 2508, 2868, 3380
BclI	TGATCA	2 cut(s)	1459, 3363
BcoDI	GTCTC	4 cut(s)	197, 651, 1072, 1109
BcuI	ACTAGT	2 cut(s)	3580, 3655
BfaI	CTAG	6 cut(s)	693, 885, 2129, 3042, 3581, 3656
BfmI	CTRYAG	8 cut(s)	375, 678, 1071, 1201, 1409, 1794, 1938, 2626
BfoI	RGCGCY	1 cut(s)	1259
BfuAI	ACCTGC	1 cut(s)	3115
BisI	GCNGC	8 cut(s)	681, 1158, 2072, 2211, 2751, 3153, 3288, 3322
BlpI	GCTNAGC	2 cut(s)	2467, 2742
BlsI	GCNGC	8 cut(s)	682, 1159, 2073, 2212, 2752, 3154, 3289, 3323
BmcAI	AGTACT	2 cut(s)	99, 392
Bme1390I	CCNGG	6 cut(s)	552, 1379, 2039, 2508, 2868, 3380
Bme18I	GGWCC	4 cut(s)	1523, 2114, 2591, 3716
BmeT110I	CYCGRG	1 cut(s)	2789
BmgT120I	GGNCC	4 cut(s)	1523, 2114, 2591, 3716
BmiI	GGNNCC	5 cut(s)	487, 1257, 1524, 2827, 2987
BmrFI	CCNGG	6 cut(s)	552, 1379, 2039, 2508, 2868, 3380
BmrI	ACTGGG	4 cut(s)	830, 976, 1556, 3510
BmsI	GCATC	1 cut(s)	2043
BmuI	ACTGGG	4 cut(s)	830, 976, 1556, 3510
BplI	GAGNNNNNCTC	2 cut(s)	3139, 3171
BpmI	CTGGAG	1 cut(s)	2225
Bpu1102I	GCTNAGC	2 cut(s)	2467, 2742
Bpu14I	TTCGAA	1 cut(s)	2584
BpuEI	CTTGAG	5 cut(s)	773, 2520, 3141, 3592, 3724
Bsa29I	ATCGAT	1 cut(s)	2120
BsaAI	YACGTR	1 cut(s)	2916
BsaHI	GRCGYC	1 cut(s)	1256
BsaJI	CCNNGG	9 cut(s)	1377, 1378, 1683, 1881, 1930, 2790, 2981, 3379, 3444
BsaWI	WCCGGW	4 cut(s)	316, 1101, 2722, 3694
BsaXI	ACNNNNNCTCC	4 cut(s)	298, 328, 3537, 3567
Bsc4I	CCNNNNNNNGG	5 cut(s)	841, 842, 1090, 1384, 2287
Bse118I	RCCGGY	2 cut(s)	488, 3694
Bse1I	ACTGG	6 cut(s)	187, 836, 971, 1551, 1683, 3505
Bse3DI	GCAATG	4 cut(s)	193, 2101, 2448, 2478
BseAI	TCCGGA	2 cut(s)	316, 2722
BseBI	CCWGG	6 cut(s)	552, 1379, 2039, 2508, 2868, 3380
BseCI	ATCGAT	1 cut(s)	2120
BseDI	CCNNGG	9 cut(s)	1377, 1378, 1683, 1881, 1930, 2790, 2981, 3379, 3444
BseGI	GGATG	8 cut(s)	426, 1693, 1877, 1968, 2034, 2807, 3525, 3546
BseLI	CCNNNNNNNGG	5 cut(s)	841, 842, 1090, 1384, 2287
BseMI	GCAATG	4 cut(s)	193, 2101, 2448, 2478
BseMII	CTCAG	5 cut(s)	728, 1350, 1572, 1632, 2646
BseNI	ACTGG	6 cut(s)	187, 836, 971, 1551, 1683, 3505
BseRI	GAGGAG	1 cut(s)	834
BseXI	GCAGC	8 cut(s)	692, 1144, 2058, 2197, 2737, 3139, 3274, 3333
BseYI	CCCAGC	1 cut(s)	416
BsgI	GTGCAG	1 cut(s)	1527
Bsh1236I	CGCG	3 cut(s)	2252, 2996, 3359
BshFI	GGCC	7 cut(s)	610, 1001, 1123, 1682, 2379, 2886, 3681
BshNI	GGYRCC	2 cut(s)	1255, 2825
BshTI	ACCGGT	1 cut(s)	3694
BshVI	ATCGAT	1 cut(s)	2120
BsiHKAI	GWGCWC	2 cut(s)	3335, 3600
BsiHKCI	CYCGRG	1 cut(s)	2789
BsiSI	CCGG	6 cut(s)	317, 489, 1102, 2723, 2923, 3695
BslFI	GGGAC	1 cut(s)	3518
BslI	CCNNNNNNNGG	5 cut(s)	841, 842, 1090, 1384, 2287
BsmAI	GTCTC	4 cut(s)	197, 651, 1072, 1109
BsmFI	GGGAC	1 cut(s)	3518
BsnI	GGCC	7 cut(s)	610, 1001, 1123, 1682, 2379, 2886, 3681
BsoBI	CYCGRG	1 cut(s)	2789
Bsp119I	TTCGAA	1 cut(s)	2584
Bsp1286I	GDGCHC	2 cut(s)	3335, 3600
Bsp13I	TCCGGA	2 cut(s)	316, 2722
Bsp1720I	GCTNAGC	2 cut(s)	2467, 2742
Bsp68I	TCGCGA	1 cut(s)	3359
BspACI	CCGC	5 cut(s)	536, 587, 2250, 2947, 3455
BspANI	GGCC	7 cut(s)	610, 1001, 1123, 1682, 2379, 2886, 3681
BspCNI	CTCAG	5 cut(s)	727, 1349, 1571, 1633, 2645
BspDI	ATCGAT	1 cut(s)	2120
BspEI	TCCGGA	2 cut(s)	316, 2722
BspFNI	CGCG	3 cut(s)	2252, 2996, 3359
BspLI	GGNNCC	5 cut(s)	487, 1257, 1524, 2827, 2987
BspMAI	CTGCAG	2 cut(s)	682, 1413
BspMI	ACCTGC	1 cut(s)	3115
BspPI	GGATC	6 cut(s)	327, 575, 754, 1093, 1150, 1526
BspT104I	TTCGAA	1 cut(s)	2584
BspT107I	GGYRCC	2 cut(s)	1255, 2825
BsrDI	GCAATG	4 cut(s)	193, 2101, 2448, 2478
BsrFI	RCCGGY	2 cut(s)	488, 3694
BsrI	ACTGG	6 cut(s)	187, 836, 971, 1551, 1683, 3505
BssAI	RCCGGY	2 cut(s)	488, 3694
BssECI	CCNNGG	9 cut(s)	1377, 1378, 1683, 1881, 1930, 2790, 2981, 3379, 3444
BssNI	GRCGYC	1 cut(s)	1256
BssT1I	CCWWGG	4 cut(s)	1683, 1881, 1930, 2981
Bst2UI	CCWGG	6 cut(s)	552, 1379, 2039, 2508, 2868, 3380
Bst4CI	ACNGT	5 cut(s)	376, 561, 985, 1231, 1568
Bst6I	CTCTTC	1 cut(s)	1158
BstACI	GRCGYC	1 cut(s)	1256
BstAPI	GCANNNNNTGC	1 cut(s)	2071
BstBAI	YACGTR	1 cut(s)	2916
BstBI	TTCGAA	1 cut(s)	2584
BstC8I	GCNNGC	4 cut(s)	2184, 2377, 2748, 2928
BstDSI	CCRYGG	1 cut(s)	3444
BstENI	CCTNNNNNAGG	1 cut(s)	1382
BstF5I	GGATG	8 cut(s)	426, 1693, 1877, 1968, 2034, 2807, 3525, 3546
BstFNI	CGCG	3 cut(s)	2252, 2996, 3359
BstH2I	RGCGCY	1 cut(s)	1259
BstHHI	GCGC	2 cut(s)	1258, 2998
BstMAI	GTCTC	4 cut(s)	197, 651, 1072, 1109
BstNI	CCWGG	6 cut(s)	552, 1379, 2039, 2508, 2868, 3380
BstNSI	RCATGY	5 cut(s)	273, 2930, 3321, 3712, 3734
BstSCI	CCNGG	6 cut(s)	550, 1377, 2037, 2506, 2866, 3378
BstSFI	CTRYAG	8 cut(s)	375, 678, 1071, 1201, 1409, 1794, 1938, 2626
BstUI	CGCG	3 cut(s)	2252, 2996, 3359
BstV1I	GCAGC	8 cut(s)	692, 1144, 2058, 2197, 2737, 3139, 3274, 3333
Bsu15I	ATCGAT	1 cut(s)	2120
BsuRI	GGCC	7 cut(s)	610, 1001, 1123, 1682, 2379, 2886, 3681
BsuTUI	ATCGAT	1 cut(s)	2120
BtgI	CCRYGG	1 cut(s)	3444
BtgZI	GCGATG	2 cut(s)	3268, 3374
BtsCI	GGATG	8 cut(s)	426, 1693, 1877, 1968, 2034, 2807, 3525, 3546
BtsIMutI	CAGTG	6 cut(s)	180, 1544, 1957, 3219, 3498, 3565
BtuMI	TCGCGA	1 cut(s)	3359
BveI	ACCTGC	1 cut(s)	3115
Cac8I	GCNNGC	4 cut(s)	2184, 2377, 2748, 2928
CaiI	CAGNNNCTG	2 cut(s)	10, 1070
CfoI	GCGC	2 cut(s)	1258, 2998
Cfr10I	RCCGGY	2 cut(s)	488, 3694
Cfr13I	GGNCC	4 cut(s)	1523, 2114, 2591, 3716
ClaI	ATCGAT	1 cut(s)	2120
Csp6I	GTAC	9 cut(s)	98, 391, 986, 2826, 2910, 3099, 3470, 3631, 3776
CspAI	ACCGGT	1 cut(s)	3694
CviQI	GTAC	9 cut(s)	98, 391, 986, 2826, 2910, 3099, 3470, 3631, 3776
DinI	GGCGCC	1 cut(s)	1257
DraI	TTTAAA	1 cut(s)	2694
DraIII	CACNNNGTG	1 cut(s)	1403
EaeI	YGGCCR	2 cut(s)	999, 1680
Eam1104I	CTCTTC	1 cut(s)	1158
EarI	CTCTTC	1 cut(s)	1158
Ecl136II	GAGCTC	1 cut(s)	3333
Eco130I	CCWWGG	4 cut(s)	1683, 1881, 1930, 2981
Eco147I	AGGCCT	1 cut(s)	610
Eco24I	GRGCYC	1 cut(s)	3335
Eco32I	GATATC	2 cut(s)	70, 1363
Eco47I	GGWCC	4 cut(s)	1523, 2114, 2591, 3716
Eco53kI	GAGCTC	1 cut(s)	3333
Eco57I	CTGAAG	4 cut(s)	1182, 2534, 2633, 3534
Eco72I	CACGTG	1 cut(s)	2916
Eco88I	CYCGRG	1 cut(s)	2789
EcoICRI	GAGCTC	1 cut(s)	3333
EcoNI	CCTNNNNNAGG	1 cut(s)	1382
EcoRI	GAATTC	1 cut(s)	384
EcoRII	CCWGG	6 cut(s)	550, 1377, 2037, 2506, 2866, 3378
EcoRV	GATATC	2 cut(s)	70, 1363
EcoT14I	CCWWGG	4 cut(s)	1683, 1881, 1930, 2981
EcoT38I	GRGCYC	1 cut(s)	3335
EgeI	GGCGCC	1 cut(s)	1257
EheI	GGCGCC	1 cut(s)	1257
ErhI	CCWWGG	4 cut(s)	1683, 1881, 1930, 2981
FalI	AAGNNNNNCTT	6 cut(s)	2364, 2396, 2698, 2730, 2961, 2993
FaqI	GGGAC	1 cut(s)	3518
FauI	CCCGC	1 cut(s)	529
FauNDI	CATATG	1 cut(s)	3248
FbaI	TGATCA	2 cut(s)	1459, 3363
FblI	GTMKAC	1 cut(s)	2492
Fnu4HI	GCNGC	8 cut(s)	681, 1158, 2072, 2211, 2751, 3153, 3288, 3322
FokI	GGATG	8 cut(s)	433, 1700, 1864, 1975, 2021, 2814, 3532, 3553
FriOI	GRGCYC	1 cut(s)	3335
Fsp4HI	GCNGC	8 cut(s)	681, 1158, 2072, 2211, 2751, 3153, 3288, 3322
FspBI	CTAG	6 cut(s)	693, 885, 2129, 3042, 3581, 3656
GlaI	GCGC	2 cut(s)	1257, 2997
GluI	GCNGC	8 cut(s)	681, 1158, 2072, 2211, 2751, 3153, 3288, 3322
GsaI	CCCAGC	1 cut(s)	420
GsuI	CTGGAG	1 cut(s)	2225
HaeII	RGCGCY	1 cut(s)	1259
HaeIII	GGCC	7 cut(s)	610, 1001, 1123, 1682, 2379, 2886, 3681
HapII	CCGG	6 cut(s)	317, 489, 1102, 2723, 2923, 3695
HhaI	GCGC	2 cut(s)	1258, 2998
Hin1I	GRCGYC	1 cut(s)	1256
Hin6I	GCGC	2 cut(s)	1256, 2996
HinP1I	GCGC	2 cut(s)	1256, 2996
HincII	GTYRAC	3 cut(s)	1621, 2493, 3130
HindII	GTYRAC	3 cut(s)	1621, 2493, 3130
HindIII	AAGCTT	2 cut(s)	163, 3084
HpaII	CCGG	6 cut(s)	317, 489, 1102, 2723, 2923, 3695
HphI	GGTGA	9 cut(s)	549, 650, 677, 769, 824, 1480, 1842, 3364, 3601
Hpy99I	CGWCG	2 cut(s)	1741, 2286
HpyCH4III	ACNGT	5 cut(s)	376, 561, 985, 1231, 1568
HpyCH4IV	ACGT	2 cut(s)	223, 2915
HpySE526I	ACGT	2 cut(s)	223, 2915
Hsp92I	GRCGYC	1 cut(s)	1256
HspAI	GCGC	2 cut(s)	1256, 2996
KasI	GGCGCC	1 cut(s)	1255
Kpn2I	TCCGGA	2 cut(s)	316, 2722
KpnI	GGTACC	1 cut(s)	2829
Ksp22I	TGATCA	2 cut(s)	1459, 3363
LmnI	GCTCC	9 cut(s)	77, 413, 485, 1198, 2515, 3003, 3330, 3545, 3727
Lsp1109I	GCAGC	8 cut(s)	692, 1144, 2058, 2197, 2737, 3139, 3274, 3333
LweI	GCATC	1 cut(s)	2043
MaeI	CTAG	6 cut(s)	693, 885, 2129, 3042, 3581, 3656
MaeII	ACGT	2 cut(s)	223, 2915
MaeIII	GTNAC	7 cut(s)	340, 942, 1287, 1594, 1631, 2555, 2942
MhlI	GDGCHC	2 cut(s)	3335, 3600
MlsI	TGGCCA	2 cut(s)	1001, 1682
MluNI	TGGCCA	2 cut(s)	1001, 1682
Mly113I	GGCGCC	1 cut(s)	1256
MlyI	GAGTC	7 cut(s)	199, 653, 1966, 2380, 2488, 2534, 2713
MmeI	TCCRAC	6 cut(s)	197, 1559, 1699, 1719, 2092, 2305
Mox20I	TGGCCA	2 cut(s)	1001, 1682
MroI	TCCGGA	2 cut(s)	316, 2722
MroXI	GAANNNNTTC	1 cut(s)	1172
MscI	TGGCCA	2 cut(s)	1001, 1682
MslI	CAYNNNNRTG	2 cut(s)	2177, 3443
Msp20I	TGGCCA	2 cut(s)	1001, 1682
MspA1I	CMGCKG	2 cut(s)	2186, 2750
MspI	CCGG	6 cut(s)	317, 489, 1102, 2723, 2923, 3695
MspR9I	CCNGG	6 cut(s)	552, 1379, 2039, 2508, 2868, 3380
MvaI	CCWGG	6 cut(s)	552, 1379, 2039, 2508, 2868, 3380
MvnI	CGCG	3 cut(s)	2252, 2996, 3359
NarI	GGCGCC	1 cut(s)	1256
NdeI	CATATG	1 cut(s)	3248
NlaIV	GGNNCC	5 cut(s)	487, 1257, 1524, 2827, 2987
NmeAIII	GCCGAG	3 cut(s)	491, 2167, 3314
NmuCI	GTSAC	2 cut(s)	1594, 2942
NruI	TCGCGA	1 cut(s)	3359
NspI	RCATGY	5 cut(s)	273, 2930, 3321, 3712, 3734
NspV	TTCGAA	1 cut(s)	2584
PaeI	GCATGC	1 cut(s)	2930
PasI	CCCWGGG	1 cut(s)	1378
PceI	AGGCCT	1 cut(s)	610
PciI	ACATGT	1 cut(s)	3730
PdmI	GAANNNNTTC	1 cut(s)	1172
PfeI	GAWTC	8 cut(s)	7, 157, 289, 428, 1499, 1847, 2586, 2636
PinAI	ACCGGT	1 cut(s)	3694
PkrI	GCNGC	8 cut(s)	682, 1159, 2073, 2212, 2752, 3154, 3289, 3323
PleI	GAGTC	7 cut(s)	199, 652, 1966, 2380, 2488, 2534, 2713
PluTI	GGCGCC	1 cut(s)	1259
PmaCI	CACGTG	1 cut(s)	2916
PmlI	CACGTG	1 cut(s)	2916
PpsI	GAGTC	7 cut(s)	199, 652, 1966, 2380, 2488, 2534, 2713
Ppu21I	YACGTR	1 cut(s)	2916
PscI	ACATGT	1 cut(s)	3730
PshBI	ATTAAT	1 cut(s)	3263
PsiI	TTATAA	1 cut(s)	2622
Psp124BI	GAGCTC	1 cut(s)	3335
Psp6I	CCWGG	6 cut(s)	550, 1377, 2037, 2506, 2866, 3378
PspCI	CACGTG	1 cut(s)	2916
PspFI	CCCAGC	1 cut(s)	416
PspGI	CCWGG	6 cut(s)	550, 1377, 2037, 2506, 2866, 3378
PspN4I	GGNNCC	5 cut(s)	487, 1257, 1524, 2827, 2987
PspPI	GGNCC	4 cut(s)	1523, 2114, 2591, 3716
PstI	CTGCAG	2 cut(s)	682, 1413
PstNI	CAGNNNCTG	2 cut(s)	10, 1070
PvuII	CAGCTG	2 cut(s)	2186, 2750
RruI	TCGCGA	1 cut(s)	3359
RsaI	GTAC	9 cut(s)	99, 392, 987, 2827, 2911, 3100, 3471, 3632, 3777
RsaNI	GTAC	9 cut(s)	98, 391, 986, 2826, 2910, 3099, 3470, 3631, 3776
RseI	CAYNNNNRTG	2 cut(s)	2177, 3443
SacI	GAGCTC	1 cut(s)	3335
SalI	GTCGAC	1 cut(s)	2491
SatI	GCNGC	8 cut(s)	681, 1158, 2072, 2211, 2751, 3153, 3288, 3322
Sau96I	GGNCC	4 cut(s)	1523, 2114, 2591, 3716
ScaI	AGTACT	2 cut(s)	99, 392
SchI	GAGTC	7 cut(s)	199, 653, 1966, 2380, 2488, 2534, 2713
ScrFI	CCNGG	6 cut(s)	552, 1379, 2039, 2508, 2868, 3380
SduI	GDGCHC	2 cut(s)	3335, 3600
SfaNI	GCATC	1 cut(s)	2043
SfcI	CTRYAG	8 cut(s)	375, 678, 1071, 1201, 1409, 1794, 1938, 2626
SfoI	GGCGCC	1 cut(s)	1257
SfuI	TTCGAA	1 cut(s)	2584
SinI	GGWCC	4 cut(s)	1523, 2114, 2591, 3716
SmiMI	CAYNNNNRTG	2 cut(s)	2177, 3443
SmlI	CTYRAG	5 cut(s)	752, 2535, 3156, 3607, 3703
SmoI	CTYRAG	5 cut(s)	752, 2535, 3156, 3607, 3703
SpeI	ACTAGT	2 cut(s)	3580, 3655
SphI	GCATGC	1 cut(s)	2930
SseBI	AGGCCT	1 cut(s)	610
SsiI	CCGC	5 cut(s)	536, 587, 2250, 2947, 3455
SspDI	GGCGCC	1 cut(s)	1255
SspI	AATATT	3 cut(s)	1282, 1472, 2569
SspMI	CTAG	6 cut(s)	693, 885, 2129, 3042, 3581, 3656
SstI	GAGCTC	1 cut(s)	3335
StuI	AGGCCT	1 cut(s)	610
StyD4I	CCNGG	6 cut(s)	550, 1377, 2037, 2506, 2866, 3378
StyI	CCWWGG	4 cut(s)	1683, 1881, 1930, 2981
TaaI	ACNGT	5 cut(s)	376, 561, 985, 1231, 1568
TaiI	ACGT	2 cut(s)	226, 2918
TaqII	GACCGA	1 cut(s)	2579
TatI	WGTACW	4 cut(s)	97, 390, 985, 3630
TfiI	GAWTC	8 cut(s)	7, 157, 289, 428, 1499, 1847, 2586, 2636
TscAI	CASTG	6 cut(s)	187, 1551, 1957, 3219, 3505, 3565
TseFI	GTSAC	2 cut(s)	1594, 2942
TseI	GCWGC	8 cut(s)	680, 1157, 2071, 2210, 2750, 3152, 3287, 3321
Tsp45I	GTSAC	2 cut(s)	1594, 2942
TspGWI	ACGGA	4 cut(s)	2299, 2303, 3086, 3433
TspRI	CASTG	6 cut(s)	187, 1551, 1957, 3219, 3505, 3565
VpaK11BI	GGWCC	4 cut(s)	1523, 2114, 2591, 3716
VspI	ATTAAT	1 cut(s)	3263
XagI	CCTNNNNNAGG	1 cut(s)	1382
XapI	RAATTY	5 cut(s)	384, 446, 898, 1717, 3161
XceI	RCATGY	5 cut(s)	273, 2930, 3321, 3712, 3734
XcmI	CCANNNNNNNNNTGG	2 cut(s)	963, 2754
XmiI	GTMKAC	1 cut(s)	2492
XmnI	GAANNNNTTC	1 cut(s)	1172
XspI	CTAG	6 cut(s)	693, 885, 2129, 3042, 3581, 3656
ZrmI	AGTACT	2 cut(s)	99, 392

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.