Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0006478

Physical Location & Seq

Genomic Coordinates Reverse (-)

30799330 .. 30814316

Gene Structure 14987 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G024790.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3861 bp

ATGGCGCTTCTCCGCCTACGGCTTGTCGTGTTCCTCTCTCTCTTCCAGGTGTTATGGATTCTGCCTCTGGCATTTGCTCAGAGTTCTATAAAGTCCAGTGTTACCAGGCATTCTGAAGAATATTGTGCTATGTATGACATTTGTGGTAAACGTACTGATGGAAAAGTATTGAACTGTCCGTACGGTTCCCCATCTGTAAAGCCAAGTGACTTACTTTCATCTAAGATCCAAAGTTTGTGTCCAACAATTACTGGAAATGTCTGTTGTACAGCAGCTCAATTTGACACATTACGGACATCGGTTCAGCAAGCAATTCCCTTTCTTGTAGGATGTCCAGCATGCTTAAGAAACTTTTTGAACCTCTTCTGTGAGCTTACCTGCTCTCCAAATCAGAGTTTATTTATCAATGTGACTTCTGTAAACAAGGTAAATAACAATATGACCGTTGGAGGGATTGATTATTACATAAGTGATACTTTTGGTGAGGGGTTGTATGACTCCTGCAAGGATGTCAAGTTTGGTACCATGAATTCTCGAGCTATGGAATTCATTGGTGCTGGTGCTCAAAATTTTAAAGAGTGGTTTACTTTTATCGGTAGACAAGCAGCTCCTAATCTGCCAGGTTCACCATATGCCATTAGATTCCGGTCAAATGCTACTGACTCATCCAAGATGATACCTATGAATGTATCTACTTATTCTTGTGGTGATAATTCACTGGGCTGTTCCTGTGGTGATTGCCCGTCATCCACAGTTTGTTCTAACACAGCTGTTCCAGTTTCCCCCAAAAAAGGGTCATGTACAGTGAAAATTGGATCTATTAAGGCAAAATGTATTGACCTTGCTGTCGCCGTTCTATATATCGTACTGGTTTCTGTGTTTTTTGGGTGGGGTTTGTTTAATCGGACTAGGAAGCAAACCCCGGCTTCTAGGAAAAAACCGTTGTGGAATAATGTAATGGATGATGGCGAAGTTCAGTCTATAAGCAGGGAGAAGATCGAGAATCCTCCAATGCAGGTTCTTGAAGATCCTCCCCACATGAGAAGCGGTGTCCAACTTTCAATTGTGCAAGGATACATGTCAAAATTTTATAGGGGATATGGGAAGTGGGCTGCTAGAAATCCAATCACATTGTTGGGTTCATCGTCTGCTATAGTTCTTCTACTTTGTCTTGGTCTTATCCGGTTCAAGGTGGAAACAAGGCCTGAGAAGCTATGGGTAGGGCCAGGGAGTAAAGCAGCAGAGGAGAAGCAATTTTTTGATAGTCACCTAGCCCCTTTTTACAGAATTGAGCAGCTTATATTAGCAACAATACCAGATGCCAAGCATGGTAAGTCACCTAGTATTGTGACGGAGGACAACATCAAGTTACTTTTTGAGATACAAAAAAAGGTTGACGGAATTAAAGCAAATTATTCTGGGTCAATGATATCTCTTGCTGATATCTGCATGAAGCCACTAGACCAAGATTGCGCCAGTCAAAGTGTCATACAGTATTTCAAATTGGATCCTGAAAATTTTGACAATTATGGTGGGATTGAACACCTAACGTATTGTTTTGAGCACTATTCCTCTGCTGACAAATGTATGAGTGCATATAAAGCTCCTCTTGATCCCAGCACTGCTTTAGGTGGTTTCTCTGGGAAAAACTATTCCGAGGCATCAGCATTTATAATAACTTATCCTGTAAACAATGCTATTAATAAAGAGGGGAATGAAACTGAAAGAGCAGTGGCATGGGAGAAGGCCTTTATTCAGTTGGCAAAGGCTGAGCTGTTGCAAATGGTGCAATCTAAAAACTTAACACTATCTTTCTCATCGGAGAGTTCCGTTGAAGAAGAACTTAAGAGAGAAAGCACTGCAGATGCTATTACAATATTGATAAGCTATCTTGTGATGTTCGCTTATATTTCTCTTACTTTGGGTGACTCTCCTCGTTTGTCTTCCTTTTACATTTCATCGAAGGTATTGCTAGGTCTCTCTGGAGTTGTTCTTGTCATGCTCTCCGTTCTTGGATCAGTGGGATTGTTCAGTGCCATTGGAGTTAAATCTACCCTAATTATTATGGAAGTCATCCCATTTCTTGTTCTAGCTGTTGGGGTAGATAATATGTGTATACTGGTTCATGCTGTCAAACGGCAACCATTGACGATGCCTTTAGAAGGGCGAATAAGCAATGCACTTGTGGAAGTTGGACCATCCATAACTCTTGCTAGTCTTTCAGAAGTTTTAGCATTTGCGGTTGGCAGCTTTATTCCTATGCCAGCTTGTCGTGTGTTCTCCATGTTTGCCGCACTAGCTGTTCTTTTGGACTTCCTCCTTCAAGTTACTGCTTTTGTAGCTCTGATTGTTCTTGATTTTCGCCGAACTGAGGATAATAGGGTTGATTGTTTCCCTTGTATGAAAGTTTCATCATATACCAACTCTGATAAAGGCATTGAAGTAAGAAAACCAGGTTTGCTGGCACGCTATATGAAGGGAATCCATGCTCCCATTCTAAGTGTTTGGGTTGTTAAGATAGTTGTCATTTCCATTTTTGTTGCGTTTTCACTGGCTAGCATTGCGTTGTGCACCAGAATTCAGCCTGGTTTGGAACAAAAAATTGTTCTTCCCCAAGATTCCTATCTTCAGGGTTATTTCAATAATATATCAGCGTATCTCCGAATTGGACCCCCACTATATTTTGTTGTGAAGAACTACAACTATAGTTCAGAATCAAGACATACAAACCAATTGTGCTCCATTAGCCAATGTGATTCAGATTCTCTTTTGAATGAGATTGCAAGAGCATCCTTAATTCCAGAATCAAGTTACATTGCTAAGCCTGCTGCTTCATGGCTTGATGATTTTCTTGTGTGGATTTCCCCAGAAGCTTTTGGGTGCTGTCGGAAGTTTACAAATGCAACTTATTGTCCTCCTGATGATCAGCCTCCTTGCTGTTCTTCCTCTGATGGTACCTGTAGCCTGGGTGGAGTGTGCAAAGATTGTACCACGTGTTTTCGTCACTCGGATCTGCATAATGGTCGTCCGTCGACCACACAATTTGAAGAAAAACTTCCATGGTTCCTCGATGCTCTGCCCTCTTCAGATTGTGCTAAAGGTGGCCATGGAGCTTACACTAGCAGTGTGGAATTGAAAGGCTATGAAAGTGGCATTATTCAAGCGTCATCATTTCGTACATATCATACACCTCTCAACAAACAGATTGACTATGTAAACTCAATGAGGGCTGCTCGAGAGCTCTGTTCTAGAGTTTCTGATTCTTTGAATATCGAGATATTCCCATATTCAGTGTTTTACATGTTCTTTGAGCAGTACCTCGATATTTGGAAAACAGCACTTATGAGCCTTTCTATAGCTATTGGTGCTGTATTTATCGTTTGCCTAGTCATCACATGCAGCTTGTGGAGTTCAGCAATTATCCTTTTGGTGTTGGCAATGATTGTTGTAGATCTCATGGGAGTGATGGCAATTCTTCACATTCAATTAAATGCAGTCTCTGTTGTTAACCTTGTTATGGCAGTGGGCATTTCTGTTGAGTTTTGTGTGCATATGACACATGCTTTCTCGGTTAGCACAGGAGATAGAGATCAACGAATAAAGGAGGCTCTGGGTACAATGGGTGCGTCTGTCTTCAGTGGAATCACACTTACGAAGTTAGTTGGGGTTATTGTACTTTGTTTCTCAAGAACAGAAGTCTTTGTGGTGTATTACTTTCAAATGTATCTAGCACTAGTTCTTCTTGGTTTCTTACATGGGCTTGTATTTTTGCCGGTGGTGTTGAGCATTTTTGGTCCCCCTTCGAGATGTCTGCTCAGGGAGCAGCAACAAGATCGGCCATCTGTACCATCACAACAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0003674 GO:0005488 GO:0005496 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005886 GO:0005887 GO:0006082 GO:0006464 GO:0006486 GO:0006629 GO:0006807 GO:0006810 GO:0006869 GO:0007034 GO:0007041 GO:0008150 GO:0008152 GO:0008202 GO:0008206 GO:0008289 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0010876 GO:0012505 GO:0015485 GO:0015850 GO:0015918 GO:0016020 GO:0016021 GO:0019538 GO:0019752 GO:0030301 GO:0031224 GO:0031226 GO:0031967 GO:0031975 GO:0032787 GO:0032934 GO:0033036 GO:0034645 GO:0036094 GO:0036211 GO:0042592 GO:0042632 GO:0043170 GO:0043178 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0048471 GO:0048878 GO:0051179 GO:0051234 GO:0055088 GO:0055092 GO:0065007 GO:0065008 GO:0070085 GO:0071702 GO:0071704 GO:0071944 GO:0097159 GO:1901135 GO:1901137 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901615

KEGG Pathways

Metabolic & Signaling

ko04142 ko04979 map04142 map04979

Pfam Domains

Protein Families

NPC1_N Patched

Protein Analysis

1286

Amino Acids

141.31

Weight (kDa)

7.67

Isoelectric Point (pI)

44.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NPC1_N	PF16414	40 - 269	7.1e-67	Niemann-Pick C1 N terminus
NPC1_MLD	PF22314	417 - 608	2.1e-38	NPC1, middle luminal domain
Patched	PF02460	631 - 784	1.3e-31	Patched SSD
Sterol-sensing	PF12349	652 - 802	4.6e-49	Sterol-sensing domain of SREBP cleavage-activation

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0006478)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G42470
fragaria_vesca	FvH4_5g25290 FvH4_5g25290 FvH4_5g25290 FvH4_5g25290 FvH4_5g25290 FvH4_5g25290 FvH4_5g25290
malus_domestica	MD06G1009400.v1.1
prunus_persica	Prupe.5G011100_v2.0.a1 Prupe.5G011100_v2.0.a1 Prupe.5G011100_v2.0.a1 Prupe.5G011100_v2.0.a1
pyrus_communis	pycom06g00780 pycom16g25890
rosa_chinensis	RchiOBHm_Chr7g0215501
rosa_laevigata	RLG00000002657
rosa_multiflora	Rmu_sc0005928.1_g000005
rosa_roxburghii	Rroxscaffold_3G00244190
rosa_rugosa	Rorug07G0154100 Rorug07G0154200
rosa_samantha	Rh7AG290300 Rh7BG282300 Rh7CG310300 Rh7DG294100
rosa_wichuraiana	Rw7G024790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1673
AasI	GACNNNNNNGTC	1 cut(s)	845
Acc36I	ACCTGC	2 cut(s)	386, 1006
Acc65I	GGTACC	2 cut(s)	521, 2954
AccB1I	GGYRCC	2 cut(s)	521, 2954
AccI	GTMKAC	3 cut(s)	598, 2116, 3032
AciI	CCGC	4 cut(s)	13, 1047, 2240, 2292
AclWI	GGATC	8 cut(s)	220, 823, 1022, 1502, 1515, 1607, 2023, 3018
AcoI	YGGCCR	2 cut(s)	3103, 3836
AcsI	RAATTY	6 cut(s)	529, 545, 568, 1085, 1516, 2577
AcuI	CTGAAG	4 cut(s)	135, 2612, 3069, 3619
AcvI	CACGTG	1 cut(s)	2994
AfiI	CCNNNNNNNGG	7 cut(s)	450, 791, 792, 1189, 2162, 2371, 3517
AflII	CTTAAG	2 cut(s)	343, 1844
AflIII	ACRYGT	3 cut(s)	1077, 2993, 3300
AhlI	ACTAGT	1 cut(s)	3733
AjnI	CCWGG	7 cut(s)	45, 104, 619, 1225, 2452, 2584, 2964
Alw21I	GWGCWC	5 cut(s)	565, 1566, 2573, 2741, 3243
Alw26I	GTCTC	2 cut(s)	1982, 3502
Alw44I	GTGCAC	1 cut(s)	2569
AlwI	GGATC	8 cut(s)	220, 823, 1022, 1502, 1515, 1607, 2023, 3018
AlwNI	CAGNNNCTG	1 cut(s)	3500
Ama87I	CYCGRG	2 cut(s)	534, 3234
AoxI	GGCC	5 cut(s)	1202, 1223, 1746, 3103, 3836
ApaLI	GTGCAC	1 cut(s)	2569
ApoI	RAATTY	6 cut(s)	529, 545, 568, 1085, 1516, 2577
ArsI	GACNNNNNNTTYG	2 cut(s)	223, 255
AseI	ATTAAT	1 cut(s)	1701
Asp700I	GAANNNNTTC	2 cut(s)	362, 3054
Asp718I	GGTACC	2 cut(s)	521, 2954
AspLEI	GCGC	2 cut(s)	7, 1475
AspS9I	GGNCC	4 cut(s)	1223, 2195, 2669, 3794
AsuC2I	CCSGG	1 cut(s)	923
AsuHPI	GGTGA	7 cut(s)	494, 618, 719, 746, 1259, 1329, 1937
AsuNHI	GCTAGC	1 cut(s)	2555
AvaI	CYCGRG	2 cut(s)	534, 3234
AvaII	GGWCC	3 cut(s)	2195, 2669, 3794
BaeGI	GKGCMC	1 cut(s)	2573
BalI	TGGCCA	1 cut(s)	3105
BamHI	GGATCC	1 cut(s)	1507
BanI	GGYRCC	2 cut(s)	521, 2954
BanII	GRGCYC	1 cut(s)	3243
BbrPI	CACGTG	1 cut(s)	2994
BbsI	GAAGAC	2 cut(s)	1935, 3625
Bbv12I	GWGCWC	5 cut(s)	565, 1566, 2573, 2741, 3243
BccI	CCATC	8 cut(s)	152, 199, 959, 2206, 2945, 3460, 3847, 3856
BceAI	ACGGC	3 cut(s)	35, 836, 2153
BcgI	CGANNNNNNTGC	8 cut(s)	1951, 1985, 3005, 3039, 3793, 3815, 3827, 3849
BciT130I	CCWGG	7 cut(s)	47, 106, 621, 1227, 2454, 2586, 2966
BciVI	GTATCC	1 cut(s)	1067
BclI	TGATCA	1 cut(s)	2923
BcnI	CCSGG	1 cut(s)	923
BcoDI	GTCTC	2 cut(s)	1982, 3502
BcuI	ACTAGT	1 cut(s)	3733
BfmI	CTRYAG	5 cut(s)	1152, 1860, 2704, 2959, 3354
BfoI	RGCGCY	1 cut(s)	8
BfrI	CTTAAG	2 cut(s)	343, 1844
BfuAI	ACCTGC	2 cut(s)	386, 1006
BfuI	GTATCC	1 cut(s)	1067
BglII	AGATCT	1 cut(s)	3451
BlpI	GCTNAGC	2 cut(s)	1770, 2820
Bme1390I	CCNGG	8 cut(s)	47, 106, 621, 923, 1227, 2454, 2586, 2966
Bme18I	GGWCC	3 cut(s)	2195, 2669, 3794
BmeT110I	CYCGRG	2 cut(s)	534, 3234
BmgT120I	GGNCC	4 cut(s)	1223, 2195, 2669, 3794
BmiI	GGNNCC	7 cut(s)	187, 523, 1509, 2671, 2956, 3065, 3796
BmrFI	CCNGG	8 cut(s)	47, 106, 621, 923, 1227, 2454, 2586, 2966
BmrI	ACTGGG	1 cut(s)	728
BmsI	GCATC	6 cut(s)	1309, 1670, 1855, 2142, 2798, 3061
BmtI	GCTAGC	1 cut(s)	2559
BmuI	ACTGGG	1 cut(s)	728
BpiI	GAAGAC	2 cut(s)	1935, 3625
BplI	GAGNNNNNCTC	2 cut(s)	3217, 3249
BpmI	CTGGAG	1 cut(s)	2004
Bpu10I	CCTNAGC	1 cut(s)	3815
Bpu1102I	GCTNAGC	2 cut(s)	1770, 2820
BpuEI	CTTGAG	1 cut(s)	3670
BpuMI	CCSGG	1 cut(s)	923
BsaAI	YACGTR	1 cut(s)	2994
BsaBI	GATNNNNATC	2 cut(s)	2622, 3588
BsaI	GGTCTC	1 cut(s)	1982
BsaJI	CCNNGG	6 cut(s)	921, 1226, 1656, 2965, 3059, 3106
BsaWI	WCCGGW	2 cut(s)	645, 1182
BsaXI	ACNNNNNCTCC	2 cut(s)	367, 397
Bsc4I	CCNNNNNNNGG	7 cut(s)	450, 791, 792, 1189, 2162, 2371, 3517
Bse118I	RCCGGY	1 cut(s)	3772
Bse1I	ACTGG	8 cut(s)	96, 256, 723, 776, 873, 1476, 2124, 2556
Bse3DI	GCAATG	4 cut(s)	2182, 2559, 2814, 3444
Bse8I	GATNNNNATC	2 cut(s)	2622, 3588
BseBI	CCWGG	7 cut(s)	47, 106, 621, 1227, 2454, 2586, 2966
BseDI	CCNNGG	6 cut(s)	921, 1226, 1656, 2965, 3059, 3106
BseGI	GGATG	8 cut(s)	335, 514, 665, 746, 967, 2073, 2198, 2789
BseJI	GATNNNNATC	2 cut(s)	2622, 3588
BseLI	CCNNNNNNNGG	7 cut(s)	450, 791, 792, 1189, 2162, 2371, 3517
BseMI	GCAATG	4 cut(s)	2182, 2559, 2814, 3444
BseMII	CTCAG	5 cut(s)	92, 1197, 1761, 2361, 3829
BseNI	ACTGG	8 cut(s)	96, 256, 723, 776, 873, 1476, 2124, 2556
BseRI	GAGGAG	3 cut(s)	1259, 1596, 1923
BseSI	GKGCMC	1 cut(s)	2573
BseYI	CCCAGC	1 cut(s)	1616
BshFI	GGCC	5 cut(s)	1204, 1225, 1748, 3105, 3838
BshNI	GGYRCC	2 cut(s)	521, 2954
BsiHKAI	GWGCWC	5 cut(s)	565, 1566, 2573, 2741, 3243
BsiHKCI	CYCGRG	2 cut(s)	534, 3234
BsiSI	CCGG	4 cut(s)	646, 923, 1183, 3773
BsiWI	CGTACG	1 cut(s)	180
BslFI	GGGAC	1 cut(s)	3780
BslI	CCNNNNNNNGG	7 cut(s)	450, 791, 792, 1189, 2162, 2371, 3517
BsmAI	GTCTC	2 cut(s)	1982, 3502
BsmFI	GGGAC	1 cut(s)	3780
BsmI	GAATGC	1 cut(s)	109
BsnI	GGCC	5 cut(s)	1204, 1225, 1748, 3105, 3838
Bso31I	GGTCTC	1 cut(s)	1982
BsoBI	CYCGRG	2 cut(s)	534, 3234
Bsp1286I	GDGCHC	5 cut(s)	565, 1566, 2573, 2741, 3243
Bsp1407I	TGTACA	2 cut(s)	266, 800
Bsp1720I	GCTNAGC	2 cut(s)	1770, 2820
Bsp19I	CCATGG	2 cut(s)	3059, 3106
BspACI	CCGC	4 cut(s)	13, 1047, 2240, 2292
BspANI	GGCC	5 cut(s)	1204, 1225, 1748, 3105, 3838
BspCNI	CTCAG	5 cut(s)	91, 1198, 1762, 2362, 3828
BspLI	GGNNCC	7 cut(s)	187, 523, 1509, 2671, 2956, 3065, 3796
BspMAI	CTGCAG	1 cut(s)	1864
BspMI	ACCTGC	2 cut(s)	386, 1006
BspOI	GCTAGC	1 cut(s)	2559
BspPI	GGATC	8 cut(s)	220, 823, 1022, 1502, 1515, 1607, 2023, 3018
BspT107I	GGYRCC	2 cut(s)	521, 2954
BspTI	CTTAAG	2 cut(s)	343, 1844
BspTNI	GGTCTC	1 cut(s)	1982
BsrDI	GCAATG	4 cut(s)	2182, 2559, 2814, 3444
BsrFI	RCCGGY	1 cut(s)	3772
BsrGI	TGTACA	2 cut(s)	266, 800
BsrI	ACTGG	8 cut(s)	96, 256, 723, 776, 873, 1476, 2124, 2556
BssAI	RCCGGY	1 cut(s)	3772
BssECI	CCNNGG	6 cut(s)	921, 1226, 1656, 2965, 3059, 3106
BssNAI	GTATAC	1 cut(s)	2117
BssT1I	CCWWGG	2 cut(s)	3059, 3106
Bst1107I	GTATAC	1 cut(s)	2117
Bst2UI	CCWGG	7 cut(s)	47, 106, 621, 1227, 2454, 2586, 2966
Bst4CI	ACNGT	7 cut(s)	176, 185, 445, 754, 805, 942, 1494
Bst6I	CTCTTC	3 cut(s)	47, 368, 3088
BstAFI	CTTAAG	2 cut(s)	343, 1844
BstAPI	GCANNNNNTGC	1 cut(s)	1786
BstAUI	TGTACA	2 cut(s)	266, 800
BstBAI	YACGTR	1 cut(s)	2994
BstC8I	GCNNGC	7 cut(s)	309, 340, 2265, 2463, 2467, 2557, 2826
BstDEI	CTNAG	8 cut(s)	78, 222, 1206, 1770, 2370, 2498, 2820, 3815
BstDSI	CCRYGG	2 cut(s)	3059, 3106
BstENI	CCTNNNNNAGG	1 cut(s)	2160
BstF5I	GGATG	8 cut(s)	335, 514, 665, 746, 967, 2073, 2198, 2789
BstH2I	RGCGCY	1 cut(s)	8
BstHHI	GCGC	2 cut(s)	7, 1475
BstMAI	GTCTC	2 cut(s)	1982, 3502
BstNI	CCWGG	7 cut(s)	47, 106, 621, 1227, 2454, 2586, 2966
BstNSI	RCATGY	5 cut(s)	342, 1081, 3304, 3399, 3563
BstSCI	CCNGG	8 cut(s)	45, 104, 619, 921, 1225, 2452, 2584, 2964
BstSFI	CTRYAG	5 cut(s)	1152, 1860, 2704, 2959, 3354
BstSLI	GKGCMC	1 cut(s)	2573
BstV2I	GAAGAC	2 cut(s)	1935, 3625
BstX2I	RGATCY	6 cut(s)	225, 815, 1027, 1507, 3010, 3451
BstYI	RGATCY	6 cut(s)	225, 815, 1027, 1507, 3010, 3451
BstZ17I	GTATAC	1 cut(s)	2117
BsuI	GTATCC	1 cut(s)	1067
BsuRI	GGCC	5 cut(s)	1204, 1225, 1748, 3105, 3838
BtgI	CCRYGG	2 cut(s)	3059, 3106
BtsCI	GGATG	8 cut(s)	335, 514, 665, 746, 967, 2073, 2198, 2789
BtsI	GCAGTG	5 cut(s)	1620, 1737, 1857, 3130, 3528
BveI	ACCTGC	2 cut(s)	386, 1006
Cac8I	GCNNGC	7 cut(s)	309, 340, 2265, 2463, 2467, 2557, 2826
CaiI	CAGNNNCTG	1 cut(s)	3500
CfoI	GCGC	2 cut(s)	7, 1475
Cfr10I	RCCGGY	1 cut(s)	3772
Cfr13I	GGNCC	4 cut(s)	1223, 2195, 2669, 3794
CseI	GACGC	2 cut(s)	3153, 3615
CsiI	ACCWGGT	1 cut(s)	2452
CspCI	CAANNNNNGTGG	4 cut(s)	1513, 1548, 3025, 3060
DdeI	CTNAG	8 cut(s)	78, 222, 1206, 1770, 2370, 2498, 2820, 3815
DraI	TTTAAA	1 cut(s)	574
DrdI	GACNNNNNNGTC	1 cut(s)	845
DseDI	GACNNNNNNGTC	1 cut(s)	845
EaeI	YGGCCR	2 cut(s)	3103, 3836
Eam1104I	CTCTTC	3 cut(s)	47, 368, 3088
EarI	CTCTTC	3 cut(s)	47, 368, 3088
Ecl136II	GAGCTC	1 cut(s)	3241
Eco130I	CCWWGG	2 cut(s)	3059, 3106
Eco147I	AGGCCT	2 cut(s)	1204, 1748
Eco24I	GRGCYC	1 cut(s)	3243
Eco31I	GGTCTC	1 cut(s)	1982
Eco32I	GATATC	2 cut(s)	1431, 1444
Eco47I	GGWCC	3 cut(s)	2195, 2669, 3794
Eco53kI	GAGCTC	1 cut(s)	3241
Eco57I	CTGAAG	4 cut(s)	135, 2612, 3069, 3619
Eco72I	CACGTG	1 cut(s)	2994
Eco88I	CYCGRG	2 cut(s)	534, 3234
EcoICRI	GAGCTC	1 cut(s)	3241
EcoNI	CCTNNNNNAGG	1 cut(s)	2160
EcoRI	GAATTC	3 cut(s)	529, 545, 2577
EcoRII	CCWGG	7 cut(s)	45, 104, 619, 1225, 2452, 2584, 2964
EcoRV	GATATC	2 cut(s)	1431, 1444
EcoT14I	CCWWGG	2 cut(s)	3059, 3106
EcoT38I	GRGCYC	1 cut(s)	3243
ErhI	CCWWGG	2 cut(s)	3059, 3106
FalI	AAGNNNNNCTT	8 cut(s)	460, 492, 1593, 1625, 1827, 1859, 3039, 3071
FaqI	GGGAC	1 cut(s)	3780
FauNDI	CATATG	2 cut(s)	631, 3552
FbaI	TGATCA	1 cut(s)	2923
FblI	GTMKAC	3 cut(s)	598, 2116, 3032
FokI	GGATG	8 cut(s)	342, 521, 652, 733, 974, 2060, 2185, 2776
FriOI	GRGCYC	1 cut(s)	3243
GlaI	GCGC	2 cut(s)	6, 1474
GsaI	CCCAGC	1 cut(s)	1620
GsuI	CTGGAG	1 cut(s)	2004
HaeII	RGCGCY	1 cut(s)	8
HaeIII	GGCC	5 cut(s)	1204, 1225, 1748, 3105, 3838
HapII	CCGG	4 cut(s)	646, 923, 1183, 3773
HgaI	GACGC	2 cut(s)	3153, 3615
HhaI	GCGC	2 cut(s)	7, 1475
Hin6I	GCGC	2 cut(s)	5, 1473
HinP1I	GCGC	2 cut(s)	5, 1473
HincII	GTYRAC	3 cut(s)	1396, 3033, 3508
HindII	GTYRAC	3 cut(s)	1396, 3033, 3508
HindIII	AAGCTT	1 cut(s)	2871
HpaI	GTTAAC	1 cut(s)	3508
HpaII	CCGG	4 cut(s)	646, 923, 1183, 3773
HphI	GGTGA	7 cut(s)	494, 618, 719, 746, 1259, 1329, 1937
Hpy99I	CGWCG	1 cut(s)	3034
HpyAV	CCTTC	6 cut(s)	1738, 1957, 2156, 2330, 2470, 3810
HpyCH4III	ACNGT	7 cut(s)	176, 185, 445, 754, 805, 942, 1494
HpyCH4IV	ACGT	3 cut(s)	151, 1550, 2993
HpyF3I	CTNAG	8 cut(s)	78, 222, 1206, 1770, 2370, 2498, 2820, 3815
HpySE526I	ACGT	3 cut(s)	151, 1550, 2993
HspAI	GCGC	2 cut(s)	5, 1473
KpnI	GGTACC	2 cut(s)	525, 2958
Ksp22I	TGATCA	1 cut(s)	2923
KspAI	GTTAAC	1 cut(s)	3508
LmnI	GCTCC	6 cut(s)	613, 1609, 2494, 2744, 3110, 3820
LweI	GCATC	6 cut(s)	1309, 1670, 1855, 2142, 2798, 3061
MabI	ACCWGGT	1 cut(s)	2452
MaeII	ACGT	3 cut(s)	151, 1550, 2993
MfeI	CAATTG	2 cut(s)	1062, 2732
MflI	RGATCY	6 cut(s)	225, 815, 1027, 1507, 3010, 3451
MhlI	GDGCHC	5 cut(s)	565, 1566, 2573, 2741, 3243
MlsI	TGGCCA	1 cut(s)	3105
MluNI	TGGCCA	1 cut(s)	3105
MlyI	GAGTC	3 cut(s)	491, 656, 1922
MmeI	TCCRAC	5 cut(s)	266, 427, 1078, 2173, 2867
Mox20I	TGGCCA	1 cut(s)	3105
MroXI	GAANNNNTTC	2 cut(s)	362, 3054
MscI	TGGCCA	1 cut(s)	3105
MslI	CAYNNNNRTG	1 cut(s)	3461
Msp20I	TGGCCA	1 cut(s)	3105
MspA1I	CMGCKG	1 cut(s)	770
MspCI	CTTAAG	2 cut(s)	343, 1844
MspI	CCGG	4 cut(s)	646, 923, 1183, 3773
MspR9I	CCNGG	8 cut(s)	47, 106, 621, 923, 1227, 2454, 2586, 2966
MunI	CAATTG	2 cut(s)	1062, 2732
Mva1269I	GAATGC	1 cut(s)	109
MvaI	CCWGG	7 cut(s)	47, 106, 621, 1227, 2454, 2586, 2966
NciI	CCSGG	1 cut(s)	923
NcoI	CCATGG	2 cut(s)	3059, 3106
NdeI	CATATG	2 cut(s)	631, 3552
NheI	GCTAGC	1 cut(s)	2555
NlaIV	GGNNCC	7 cut(s)	187, 523, 1509, 2671, 2956, 3065, 3796
NmuCI	GTSAC	7 cut(s)	206, 409, 1265, 1335, 1348, 1925, 3002
NspI	RCATGY	5 cut(s)	342, 1081, 3304, 3399, 3563
PaeI	GCATGC	1 cut(s)	342
PaeR7I	CTCGAG	2 cut(s)	534, 3234
PceI	AGGCCT	2 cut(s)	1204, 1748
PciI	ACATGT	2 cut(s)	1077, 3300
PctI	GAATGC	1 cut(s)	109
PdmI	GAANNNNTTC	2 cut(s)	362, 3054
Pfl23II	CGTACG	1 cut(s)	180
PleI	GAGTC	3 cut(s)	491, 656, 1922
PmaCI	CACGTG	1 cut(s)	2994
PmlI	CACGTG	1 cut(s)	2994
PpsI	GAGTC	3 cut(s)	491, 656, 1922
Ppu21I	YACGTR	1 cut(s)	2994
PscI	ACATGT	2 cut(s)	1077, 3300
PshBI	ATTAAT	1 cut(s)	1701
PsiI	TTATAA	1 cut(s)	1673
Psp124BI	GAGCTC	1 cut(s)	3243
Psp6I	CCWGG	7 cut(s)	45, 104, 619, 1225, 2452, 2584, 2964
PspCI	CACGTG	1 cut(s)	2994
PspFI	CCCAGC	1 cut(s)	1616
PspGI	CCWGG	7 cut(s)	45, 104, 619, 1225, 2452, 2584, 2964
PspLI	CGTACG	1 cut(s)	180
PspN4I	GGNNCC	7 cut(s)	187, 523, 1509, 2671, 2956, 3065, 3796
PspPI	GGNCC	4 cut(s)	1223, 2195, 2669, 3794
PsrI	GAACNNNNNNTAC	2 cut(s)	164, 196
PstI	CTGCAG	1 cut(s)	1864
PstNI	CAGNNNCTG	1 cut(s)	3500
PsuI	RGATCY	6 cut(s)	225, 815, 1027, 1507, 3010, 3451
PvuII	CAGCTG	1 cut(s)	770
RseI	CAYNNNNRTG	1 cut(s)	3461
SacI	GAGCTC	1 cut(s)	3243
SalI	GTCGAC	1 cut(s)	3031
Sau96I	GGNCC	4 cut(s)	1223, 2195, 2669, 3794
SchI	GAGTC	3 cut(s)	491, 656, 1922
ScrFI	CCNGG	8 cut(s)	47, 106, 621, 923, 1227, 2454, 2586, 2966
SduI	GDGCHC	5 cut(s)	565, 1566, 2573, 2741, 3243
SexAI	ACCWGGT	1 cut(s)	2452
SfaNI	GCATC	6 cut(s)	1309, 1670, 1855, 2142, 2798, 3061
SfcI	CTRYAG	5 cut(s)	1152, 1860, 2704, 2959, 3354
Sfr274I	CTCGAG	2 cut(s)	534, 3234
SinI	GGWCC	3 cut(s)	2195, 2669, 3794
SlaI	CTCGAG	2 cut(s)	534, 3234
SmiMI	CAYNNNNRTG	1 cut(s)	3461
SmlI	CTYRAG	5 cut(s)	343, 534, 1844, 3234, 3685
SmoI	CTYRAG	5 cut(s)	343, 534, 1844, 3234, 3685
SpeI	ACTAGT	1 cut(s)	3733
SphI	GCATGC	1 cut(s)	342
SseBI	AGGCCT	2 cut(s)	1204, 1748
SsiI	CCGC	4 cut(s)	13, 1047, 2240, 2292
SspI	AATATT	2 cut(s)	122, 1878
SstI	GAGCTC	1 cut(s)	3243
StuI	AGGCCT	2 cut(s)	1204, 1748
StyD4I	CCNGG	8 cut(s)	45, 104, 619, 921, 1225, 2452, 2584, 2964
StyI	CCWWGG	2 cut(s)	3059, 3106
TaaI	ACNGT	7 cut(s)	176, 185, 445, 754, 805, 942, 1494
TaiI	ACGT	3 cut(s)	154, 1553, 2996
TaqI	TCGA	9 cut(s)	535, 999, 1961, 3032, 3069, 3235, 3273, 3321, 3803
TatI	WGTACW	3 cut(s)	266, 800, 3673
TauI	GCSGC	1 cut(s)	2294
TseFI	GTSAC	7 cut(s)	206, 409, 1265, 1335, 1348, 1925, 3002
Tsp45I	GTSAC	7 cut(s)	206, 409, 1265, 1335, 1348, 1925, 3002
TspGWI	ACGGA	7 cut(s)	168, 307, 1367, 1413, 1819, 1996, 3018
Vha464I	CTTAAG	2 cut(s)	343, 1844
VneI	GTGCAC	1 cut(s)	2569
VpaK11BI	GGWCC	3 cut(s)	2195, 2669, 3794
VspI	ATTAAT	1 cut(s)	1701
XagI	CCTNNNNNAGG	1 cut(s)	2160
XapI	RAATTY	6 cut(s)	529, 545, 568, 1085, 1516, 2577
XbaI	TCTAGA	1 cut(s)	3248
XceI	RCATGY	5 cut(s)	342, 1081, 3304, 3399, 3563
XhoI	CTCGAG	2 cut(s)	534, 3234
XmiI	GTMKAC	3 cut(s)	598, 2116, 3032
XmnI	GAANNNNTTC	2 cut(s)	362, 3054

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.