Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0006478

Physical Location & Seq

Genomic Coordinates Reverse (-)

16767164 .. 16782486

Gene Structure 15323 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g25290.t6

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3855 bp

ATGGCGCTCCTCCGCGTACGGCACGTCGTTTTCCTCTCTCTCTTCCAGGTGCTATGGATTCTACCTGTGGTGTATGCTCAGAGTTCTATAAGTTCCAATGCAACAAGGCATTCTGAAGAATATTGTGCTATGTATGACATTTGCGGTAAACGTAGTGATGGAAAAGTATTGAACTGTCCGTTCGGTTCCCCATCTGTAAAGCCAAGTGACTTGCTTTCATCTAAGATTCAAAGTTTATGTCCAACAATTACTGGAAATGTCTGTTGTACAGCAGCCCAGTTTGACACATTACGTGCATCGGTTCAGCAAGCAATTCCCTTCCTTGTAGGTTGTCCAGCATGCTTAAGAAACTTTTTGAACCTCTTCTGTGAGCTTACCTGCTCTCCAAATCAGAGTTTATTTATCAATGTGACATCTGTAAACAAGGTAAATAACAATATGACCATTGGAGGGATTGACTTTTACATAACTGATGCTTTTGGTGAGGGTCTGTATGACTCCTGCAAGGATGTCAAGTTTGGTACTATGAATTCTCGAGCTATGGAATTCATTGGTGCTGGTGCTCAGAATTTTAAAGAGTGGTTTACTTTTATTGGTAGACAAGCAGCTCCTAACCTGCCAGGTTCACCATATGCCATTAGATTCCGTTCAAATGCTACTGACTCATCCGAGATGGTACCTATGAATGTGTCTACTTATTCTTGTGGTGATAGTTCACTGGGCTGTTCCTGTGGTGATTGCCCCTTATCCACTGTTTGTTCTAACACAGCTGCTCCAGTTTCCCCCAAAAAAGTGTCATGTGCAGTGAAAATTGGTTCTATTAAGGCCAAATGTATTGACCTTGCAGTTGCAATTCTATATATCGTGCTGCTTTCTGTGTTTTTTGGGTGGGGTTTGTTTCATCGGACTAGGAAGCAAAGCCCAGCTTCTAAGACAAAACCGTTGTGGAATAATGTAGTGGATGATGGTGAAGTTCAGTCTATAAGAAGGGAGAAGAATCCTCCAATGCAGGTGCTTGAAGATCCTGTCCACATGAGAAGCGGTGTCCAACTTTCAATTGTGCAAGGATACATGTCAAAATTTTACAGGGGATATGGGAAGTGGGTTGCTAGAAATCCAATCACATTGTTGGGTTCATCATCAGCTATAGTTCTTCTGCTTTGTCTTGGTCTTATCCGTTTCAAGGTGGAAACAAGGCCTGAGAAGCTATGGGTAGGGCCTGGGAGTAAAGCAGCAGAGGAGAAGCAATTTTTTGATAATCACCTAGCCCCTTTTTACAGAATTGAGCAGCTTATAATAGCAACAATGCCTGATGTCAAGCATGGTAAGGCACCTAGTATTGTGACGGAGGACAACATTAAGTTACTTTTTGAGATAGAAAAAAAGGTCGATGGAATTAAAGCAAATTATTCCGGGTCAATGGTATCTCTTTCTGATATCTGCATGAAGCCACTAGACCAAGACTGCGCCAGTCAAAGTGTCATACAGTATTTCAAATTGGATCCTGAAAATTATGACAATTATGGTGGGATTGAACACCTAACGTACTGTTTTGAGCACTATTCCTCTGCTGATGAATGTATGAGTGCCTATAAAGCTCCTCTTGATCCCAGCACTGCGTTAGGTGGTTTCTCAGGGAAAAACTATTCCGAGGCATCGGCGTTTTTGATTACTTATCCTGTAATTAATGCTATTAATAAAGAGGGGAATGAAACTGAAAGAGCAGTTGCATGGGAGAAGGCTTTTATTGAGTTAGCAAAGGGTGAGTTGTTGCAAATGGTGCAATCCAAAAATTTAACACTATCTTTCTCATCGGAGAGTTCCATCGAAGAAGAACTTAAGAGAGAAAGCACTGCAGATGCCATTACAATATTGATAAGCTATCTTGTGATGTTCGCTTATATTTCTCTTACACTGGGTGACTCTCCTCGTTTGTCTTCCTTCTATATTTCATCGAAGGTATTGCTAGGTCTCTCAGGAGTTGTTCTTGTCATGCTCTCCGTTCTTGGATCAGTGGGATTTTTCAGTGCCATTGGAGTTAAATCTACCCTAATTATTATGGAAGTCATCCCATTTCTAGTTCTAGCTGTCGGGGTAGATAATATGTGTATTCTGGTTCATGCTGTCAAACGGCAACCATTGACATTGCCTTTAGAAGTGCGAATAAGCAATGCACTTGTGGAAGTTGGACCATCCATAACTCTTGCTAGTCTATCAGAAGTTTTAGCATTTGCGGTTGGCAGCTTTATTCCTATGCCAGCTTGTCGTGTGTTCTCCATGTTTGCTGCACTAGCTGTTCTATTGGACTTCCTCCTTCAAGTTACTGCCTTTGTAGCTCTGATTGTTCTTGATTTCCGCCGAACTGAGGATAAGAGGGTTGATTGTTTCCCATGTATGAAAATCTCATCATATTCCAACTCTGATAAAGGCACTGAAGAAAGAAAACGCGGTTTGCTGGCACGCTATATGAAGGAGATCCATGCTCCCATTCTAAGTCTTTGGATTGTCAAGATAGTTGTCATTTCTATTTTTGTTGCGTTTTCTCTCGCAAGCATTGCATTGTGCACCAGAATTCAGCCAGGTTTGGAACAGAAAATTGTTCTTCCCCAAGATTCCTATCTTCAGGGTTATTTCAATAATATATCGGAGTACCTCCGAATTGGACCCCCACTATACTTTGTTGTGAAGAACTTCAACTATAGTTCAGAATCAAGACATACAAACCAATTGTGCTCCATTAGCCAATGTGATTCAGAATCTCTTTTGAATGAGATTGCAAGAGCATCCTTAATTCCAGAATCAAGTTACGTTGCTAAGCCTGCTGCTTCATGGCTTGATGATTTTCTTGTGTGGATCTCCCCAGAAGCTTTTGGGTGCTGTCGGAAGTTTACAAATGCAACGTATTGTCCTCCGGATGATCAGCCTCCTTGCTGTTCTTCCTCTGATGGTTCCTGTAGCCTGGGTGGAGTATGCAAAGACTGTACTACGTGTTTTCGTCACTCAGATCTGCATAATGGTCGTCCATCTACCACACAATTTAAAGAAAAACTTCCATGGTTCCTTGAAGCTCTACCCTCTTCGGATTGTGCTAAAGGTGGCCATGGAGCTTACACTAGCAGTGTAGAATTGAAAGGCTATGAACGTGGCATTATTCAAGCATCATCATTTCGTACATATCATACACCTCTCAACAAACAGGTTGACTATGTAAACTCAATGAGGGCCACTCGAGAGCTCTGTTCTAGAATTTCGGATTCTTTGAAGATTGAGGTATTCCCATATTCGGTGTTTTACATGTTCTTTGAGCAGTATCTTGATATTTGGAAAACAGCACTTATGAGCCTTTCTATAGCTATTGGCGCTGTATTTATCGTGTGCCTAGTCATCACATGCAGCTTGTGGAGTTCGGGAATTATCCTATTGGTGTTGGCAATGATTGTTGTAGATCTCATGGGCGTGATGGCAATTCTTGACATTCAGTTAAATGCAGTCTCTGTTGTTAACCTTGTAATGGCAGTGGGCATTTCTGTTGAGTTTTGTGTGCATATGACGCATGCTTTCTCGGTTAGCACTGGAGATAGAGATCAACGAATAAAGGAGGCTCTGGCTACAATGGGTGCATCTGTCTTCAGCGGAATCACACTTACAAAGTTAGTTGGGGTTATTGTGCTTTGTTTCTCAAGAACAGAAATCTTTGTGGTGTATTACTTTCAAATGTATCTAGCGTTAGTTCTTCTTGGTTTCTTACATGGGCTAGTATTTTTGCCGGTGGTGTTGAGCATGTTTGGTCCACCTTCAAGATGTGTGCTCAGGGAGCAGCAACAAGATCGGCCATCTGTACCATCACAACAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0003674 GO:0005488 GO:0005496 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005886 GO:0005887 GO:0006082 GO:0006464 GO:0006486 GO:0006629 GO:0006807 GO:0006810 GO:0006869 GO:0007034 GO:0007041 GO:0008150 GO:0008152 GO:0008202 GO:0008206 GO:0008289 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0010876 GO:0012505 GO:0015485 GO:0015850 GO:0015918 GO:0016020 GO:0016021 GO:0019538 GO:0019752 GO:0030301 GO:0031224 GO:0031226 GO:0031967 GO:0031975 GO:0032787 GO:0032934 GO:0033036 GO:0034645 GO:0036094 GO:0036211 GO:0042592 GO:0042632 GO:0043170 GO:0043178 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0048471 GO:0048878 GO:0051179 GO:0051234 GO:0055088 GO:0055092 GO:0065007 GO:0065008 GO:0070085 GO:0071702 GO:0071704 GO:0071944 GO:0097159 GO:1901135 GO:1901137 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901615

KEGG Pathways

Metabolic & Signaling

ko04142 ko04979 map04142 map04979

Pfam Domains

Protein Families

NPC1_N Patched

Protein Analysis

1285

Amino Acids

141.47

Weight (kDa)

6.95

Isoelectric Point (pI)

47.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NPC1_N	PF16414	40 - 269	4.5e-65	Niemann-Pick C1 N terminus
NPC1_MLD	PF22314	415 - 606	5.8e-36	NPC1, middle luminal domain
Patched	PF02460	629 - 782	2.3e-31	Patched SSD
Sterol-sensing	PF12349	650 - 800	2.7e-48	Sterol-sensing domain of SREBP cleavage-activation

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0006478)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G42470
fragaria_vesca	FvH4_5g25290 FvH4_5g25290 FvH4_5g25290 FvH4_5g25290 FvH4_5g25290 FvH4_5g25290 FvH4_5g25290
malus_domestica	MD06G1009400.v1.1
prunus_persica	Prupe.5G011100_v2.0.a1 Prupe.5G011100_v2.0.a1 Prupe.5G011100_v2.0.a1 Prupe.5G011100_v2.0.a1
pyrus_communis	pycom06g00780 pycom16g25890
rosa_chinensis	RchiOBHm_Chr7g0215501
rosa_laevigata	RLG00000002657
rosa_multiflora	Rmu_sc0005928.1_g000005
rosa_roxburghii	Rroxscaffold_3G00244190
rosa_rugosa	Rorug07G0154100 Rorug07G0154200
rosa_samantha	Rh7AG290300 Rh7BG282300 Rh7CG310300 Rh7DG294100
rosa_wichuraiana	Rw7G024790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1295
AarI	CACCTGC	1 cut(s)	1000
Acc36I	ACCTGC	3 cut(s)	386, 624, 1000
Acc65I	GGTACC	1 cut(s)	676
AccB1I	GGYRCC	2 cut(s)	676, 1330
AccI	GTMKAC	2 cut(s)	598, 692
AccII	CGCG	2 cut(s)	15, 2448
AccIII	TCCGGA	1 cut(s)	2911
AciI	CCGC	7 cut(s)	13, 144, 1041, 2234, 2356, 2448, 3633
AclWI	GGATC	7 cut(s)	1016, 1496, 1509, 1601, 2017, 2470, 2861
AcoI	YGGCCR	2 cut(s)	3097, 3830
AcsI	RAATTY	7 cut(s)	529, 545, 568, 1079, 1792, 2571, 3246
AcuI	CTGAAG	4 cut(s)	135, 2454, 2606, 3613
AdeI	CACNNNGTG	1 cut(s)	1919
AfaI	GTAC	9 cut(s)	18, 268, 523, 678, 1547, 2651, 2983, 3172, 3840
AfiI	CCNNNNNNNGG	5 cut(s)	450, 1183, 2365, 3283, 3511
AflII	CTTAAG	2 cut(s)	343, 1838
AflIII	ACRYGT	3 cut(s)	1071, 2987, 3294
AjiI	CACGTC	1 cut(s)	25
AjnI	CCWGG	5 cut(s)	45, 619, 1219, 2578, 2958
Alw21I	GWGCWC	6 cut(s)	565, 1560, 2567, 2735, 3237, 3810
Alw26I	GTCTC	2 cut(s)	1976, 3496
Alw44I	GTGCAC	1 cut(s)	2563
AlwI	GGATC	7 cut(s)	1016, 1496, 1509, 1601, 2017, 2470, 2861
AlwNI	CAGNNNCTG	1 cut(s)	3494
Ama87I	CYCGRG	2 cut(s)	534, 3228
Aor13HI	TCCGGA	1 cut(s)	2911
AoxI	GGCC	6 cut(s)	825, 1196, 1217, 3097, 3222, 3830
ApaLI	GTGCAC	1 cut(s)	2563
ApoI	RAATTY	7 cut(s)	529, 545, 568, 1079, 1792, 2571, 3246
ArsI	GACNNNNNNTTYG	2 cut(s)	223, 255
AseI	ATTAAT	2 cut(s)	1686, 1695
Asp700I	GAANNNNTTC	3 cut(s)	362, 2690, 3048
Asp718I	GGTACC	1 cut(s)	676
AspLEI	GCGC	3 cut(s)	7, 1469, 3362
AspS9I	GGNCC	5 cut(s)	1217, 2189, 2663, 3222, 3788
AsuC2I	CCSGG	1 cut(s)	1414
AsuHPI	GGTGA	8 cut(s)	494, 618, 719, 746, 980, 1253, 1775, 1931
AvaI	CYCGRG	2 cut(s)	534, 3228
AvaII	GGWCC	3 cut(s)	2189, 2663, 3788
BaeGI	GKGCMC	1 cut(s)	2567
BalI	TGGCCA	1 cut(s)	3099
BamHI	GGATCC	1 cut(s)	1501
BanI	GGYRCC	2 cut(s)	676, 1330
BanII	GRGCYC	1 cut(s)	3237
BbsI	GAAGAC	2 cut(s)	1929, 3619
Bbv12I	GWGCWC	6 cut(s)	565, 1560, 2567, 2735, 3237, 3810
BceAI	ACGGC	2 cut(s)	35, 2147
BcgI	CGANNNNNNTGC	6 cut(s)	1945, 1979, 2999, 3033, 3809, 3843
BciT130I	CCWGG	5 cut(s)	47, 621, 1221, 2580, 2960
BciVI	GTATCC	1 cut(s)	1061
BclI	TGATCA	1 cut(s)	2917
BcnI	CCSGG	1 cut(s)	1414
BcoDI	GTCTC	2 cut(s)	1976, 3496
BfmI	CTRYAG	5 cut(s)	1146, 1854, 2698, 2953, 3348
BfoI	RGCGCY	2 cut(s)	8, 3363
BfrI	CTTAAG	2 cut(s)	343, 1838
BfuAI	ACCTGC	3 cut(s)	386, 624, 1000
BfuI	GTATCC	1 cut(s)	1061
BglII	AGATCT	2 cut(s)	3004, 3445
BlpI	GCTNAGC	1 cut(s)	2814
Bme1390I	CCNGG	6 cut(s)	47, 621, 1221, 1414, 2580, 2960
Bme18I	GGWCC	3 cut(s)	2189, 2663, 3788
BmeT110I	CYCGRG	2 cut(s)	534, 3228
BmgBI	CACGTC	1 cut(s)	25
BmgT120I	GGNCC	5 cut(s)	1217, 2189, 2663, 3222, 3788
BmiI	GGNNCC	7 cut(s)	187, 678, 1332, 1503, 2665, 2950, 3059
BmrFI	CCNGG	6 cut(s)	47, 621, 1221, 1414, 2580, 2960
BmrI	ACTGGG	3 cut(s)	271, 728, 1925
BmsI	GCATC	7 cut(s)	305, 463, 1664, 1849, 2792, 3167, 3629
BmuI	ACTGGG	3 cut(s)	271, 728, 1925
BpiI	GAAGAC	2 cut(s)	1929, 3619
BplI	GAGNNNNNCTC	2 cut(s)	3211, 3243
BpmI	CTGGAG	2 cut(s)	759, 3594
Bpu10I	CCTNAGC	1 cut(s)	3809
Bpu1102I	GCTNAGC	1 cut(s)	2814
BpuEI	CTTGAG	1 cut(s)	3664
BpuMI	CCSGG	1 cut(s)	1414
BsaAI	YACGTR	2 cut(s)	293, 2988
BsaBI	GATNNNNATC	2 cut(s)	2616, 3582
BsaI	GGTCTC	1 cut(s)	1976
BsaJI	CCNNGG	5 cut(s)	1220, 1650, 2959, 3053, 3100
BsaWI	WCCGGW	1 cut(s)	2911
BsaXI	ACNNNNNCTCC	4 cut(s)	367, 397, 757, 787
Bsc4I	CCNNNNNNNGG	5 cut(s)	450, 1183, 2365, 3283, 3511
Bse118I	RCCGGY	1 cut(s)	3766
Bse1I	ACTGG	7 cut(s)	256, 277, 723, 776, 1470, 1920, 3577
Bse3DI	GCAATG	4 cut(s)	2144, 2176, 2553, 3438
Bse8I	GATNNNNATC	2 cut(s)	2616, 3582
BseAI	TCCGGA	1 cut(s)	2911
BseBI	CCWGG	5 cut(s)	47, 621, 1221, 2580, 2960
BseDI	CCNNGG	5 cut(s)	1220, 1650, 2959, 3053, 3100
BseGI	GGATG	7 cut(s)	514, 665, 967, 2067, 2192, 2783, 2920
BseJI	GATNNNNATC	2 cut(s)	2616, 3582
BseLI	CCNNNNNNNGG	5 cut(s)	450, 1183, 2365, 3283, 3511
BseMI	GCAATG	4 cut(s)	2144, 2176, 2553, 3438
BseMII	CTCAG	8 cut(s)	92, 578, 1191, 1647, 1989, 2355, 3015, 3823
BseNI	ACTGG	7 cut(s)	256, 277, 723, 776, 1470, 1920, 3577
BseRI	GAGGAG	3 cut(s)	1253, 1590, 1917
BseSI	GKGCMC	1 cut(s)	2567
BseYI	CCCAGC	2 cut(s)	922, 1610
BsgI	GTGCAG	2 cut(s)	822, 2271
Bsh1236I	CGCG	2 cut(s)	15, 2448
BshFI	GGCC	6 cut(s)	827, 1198, 1219, 3099, 3224, 3832
BshNI	GGYRCC	2 cut(s)	676, 1330
BsiHKAI	GWGCWC	6 cut(s)	565, 1560, 2567, 2735, 3237, 3810
BsiHKCI	CYCGRG	2 cut(s)	534, 3228
BsiSI	CCGG	3 cut(s)	1413, 2912, 3767
BsiWI	CGTACG	1 cut(s)	16
BslI	CCNNNNNNNGG	5 cut(s)	450, 1183, 2365, 3283, 3511
BsmAI	GTCTC	2 cut(s)	1976, 3496
BsmI	GAATGC	1 cut(s)	109
BsnI	GGCC	6 cut(s)	827, 1198, 1219, 3099, 3224, 3832
Bso31I	GGTCTC	1 cut(s)	1976
BsoBI	CYCGRG	2 cut(s)	534, 3228
Bsp1286I	GDGCHC	6 cut(s)	565, 1560, 2567, 2735, 3237, 3810
Bsp13I	TCCGGA	1 cut(s)	2911
Bsp1407I	TGTACA	1 cut(s)	266
Bsp1720I	GCTNAGC	1 cut(s)	2814
Bsp19I	CCATGG	2 cut(s)	3053, 3100
BspACI	CCGC	7 cut(s)	13, 144, 1041, 2234, 2356, 2448, 3633
BspANI	GGCC	6 cut(s)	827, 1198, 1219, 3099, 3224, 3832
BspCNI	CTCAG	8 cut(s)	91, 577, 1192, 1646, 1988, 2356, 3014, 3822
BspEI	TCCGGA	1 cut(s)	2911
BspFNI	CGCG	2 cut(s)	15, 2448
BspLI	GGNNCC	7 cut(s)	187, 678, 1332, 1503, 2665, 2950, 3059
BspMAI	CTGCAG	1 cut(s)	1858
BspMI	ACCTGC	3 cut(s)	386, 624, 1000
BspPI	GGATC	7 cut(s)	1016, 1496, 1509, 1601, 2017, 2470, 2861
BspT107I	GGYRCC	2 cut(s)	676, 1330
BspTI	CTTAAG	2 cut(s)	343, 1838
BspTNI	GGTCTC	1 cut(s)	1976
BsrDI	GCAATG	4 cut(s)	2144, 2176, 2553, 3438
BsrFI	RCCGGY	1 cut(s)	3766
BsrGI	TGTACA	1 cut(s)	266
BsrI	ACTGG	7 cut(s)	256, 277, 723, 776, 1470, 1920, 3577
BssAI	RCCGGY	1 cut(s)	3766
BssECI	CCNNGG	5 cut(s)	1220, 1650, 2959, 3053, 3100
BssT1I	CCWWGG	2 cut(s)	3053, 3100
Bst2UI	CCWGG	5 cut(s)	47, 621, 1221, 2580, 2960
Bst4CI	ACNGT	6 cut(s)	176, 754, 942, 1488, 1550, 2981
Bst6I	CTCTTC	3 cut(s)	47, 368, 3082
BstAFI	CTTAAG	2 cut(s)	343, 1838
BstAPI	GCANNNNNTGC	2 cut(s)	1780, 2555
BstAUI	TGTACA	1 cut(s)	266
BstBAI	YACGTR	2 cut(s)	293, 2988
BstC8I	GCNNGC	8 cut(s)	309, 340, 2259, 2457, 2461, 2551, 2820, 3555
BstDSI	CCRYGG	2 cut(s)	3053, 3100
BstF5I	GGATG	7 cut(s)	514, 665, 967, 2067, 2192, 2783, 2920
BstFNI	CGCG	2 cut(s)	15, 2448
BstH2I	RGCGCY	2 cut(s)	8, 3363
BstHHI	GCGC	3 cut(s)	7, 1469, 3362
BstMAI	GTCTC	2 cut(s)	1976, 3496
BstNI	CCWGG	5 cut(s)	47, 621, 1221, 2580, 2960
BstNSI	RCATGY	6 cut(s)	342, 1075, 3298, 3393, 3557, 3784
BstSCI	CCNGG	6 cut(s)	45, 619, 1219, 1412, 2578, 2958
BstSFI	CTRYAG	5 cut(s)	1146, 1854, 2698, 2953, 3348
BstSLI	GKGCMC	1 cut(s)	2567
BstUI	CGCG	2 cut(s)	15, 2448
BstV2I	GAAGAC	2 cut(s)	1929, 3619
BstX2I	RGATCY	6 cut(s)	1021, 1501, 2475, 2853, 3004, 3445
BstYI	RGATCY	6 cut(s)	1021, 1501, 2475, 2853, 3004, 3445
BsuI	GTATCC	1 cut(s)	1061
BsuRI	GGCC	6 cut(s)	827, 1198, 1219, 3099, 3224, 3832
BtgI	CCRYGG	2 cut(s)	3053, 3100
BtrI	CACGTC	1 cut(s)	25
BtsCI	GGATG	7 cut(s)	514, 665, 967, 2067, 2192, 2783, 2920
BtsI	GCAGTG	5 cut(s)	810, 1614, 1851, 3124, 3522
BveI	ACCTGC	3 cut(s)	386, 624, 1000
Cac8I	GCNNGC	8 cut(s)	309, 340, 2259, 2457, 2461, 2551, 2820, 3555
CaiI	CAGNNNCTG	1 cut(s)	3494
CfoI	GCGC	3 cut(s)	7, 1469, 3362
Cfr10I	RCCGGY	1 cut(s)	3766
Cfr13I	GGNCC	5 cut(s)	1217, 2189, 2663, 3222, 3788
CseI	GACGC	1 cut(s)	3559
Csp6I	GTAC	9 cut(s)	17, 267, 522, 677, 1546, 2650, 2982, 3171, 3839
CspCI	CAANNNNNGTGG	2 cut(s)	1507, 1542
CviQI	GTAC	9 cut(s)	17, 267, 522, 677, 1546, 2650, 2982, 3171, 3839
DraI	TTTAAA	2 cut(s)	574, 3040
DraIII	CACNNNGTG	1 cut(s)	1919
EaeI	YGGCCR	2 cut(s)	3097, 3830
Eam1104I	CTCTTC	3 cut(s)	47, 368, 3082
EarI	CTCTTC	3 cut(s)	47, 368, 3082
EciI	GGCGGA	1 cut(s)	2345
Ecl136II	GAGCTC	1 cut(s)	3235
Eco130I	CCWWGG	2 cut(s)	3053, 3100
Eco147I	AGGCCT	1 cut(s)	1198
Eco24I	GRGCYC	1 cut(s)	3237
Eco31I	GGTCTC	1 cut(s)	1976
Eco32I	GATATC	1 cut(s)	1438
Eco47I	GGWCC	3 cut(s)	2189, 2663, 3788
Eco53kI	GAGCTC	1 cut(s)	3235
Eco57I	CTGAAG	4 cut(s)	135, 2454, 2606, 3613
Eco88I	CYCGRG	2 cut(s)	534, 3228
EcoICRI	GAGCTC	1 cut(s)	3235
EcoO109I	RGGNCCY	1 cut(s)	1217
EcoRI	GAATTC	3 cut(s)	529, 545, 2571
EcoRII	CCWGG	5 cut(s)	45, 619, 1219, 2578, 2958
EcoRV	GATATC	1 cut(s)	1438
EcoT14I	CCWWGG	2 cut(s)	3053, 3100
EcoT38I	GRGCYC	1 cut(s)	3237
ErhI	CCWWGG	2 cut(s)	3053, 3100
FalI	AAGNNNNNCTT	8 cut(s)	910, 942, 1587, 1619, 1821, 1853, 3033, 3065
FauNDI	CATATG	2 cut(s)	631, 3546
FbaI	TGATCA	1 cut(s)	2917
FblI	GTMKAC	2 cut(s)	598, 692
FokI	GGATG	7 cut(s)	521, 652, 974, 2054, 2179, 2770, 2927
FriOI	GRGCYC	1 cut(s)	3237
GlaI	GCGC	3 cut(s)	6, 1468, 3361
GsaI	CCCAGC	2 cut(s)	926, 1614
GsuI	CTGGAG	2 cut(s)	759, 3594
HaeII	RGCGCY	2 cut(s)	8, 3363
HaeIII	GGCC	6 cut(s)	827, 1198, 1219, 3099, 3224, 3832
HapII	CCGG	3 cut(s)	1413, 2912, 3767
HgaI	GACGC	1 cut(s)	3559
HhaI	GCGC	3 cut(s)	7, 1469, 3362
Hin6I	GCGC	3 cut(s)	5, 1467, 3360
HinP1I	GCGC	3 cut(s)	5, 1467, 3360
HincII	GTYRAC	2 cut(s)	3202, 3502
HindII	GTYRAC	2 cut(s)	3202, 3502
HindIII	AAGCTT	1 cut(s)	2865
HpaI	GTTAAC	1 cut(s)	3502
HpaII	CCGG	3 cut(s)	1413, 2912, 3767
HphI	GGTGA	8 cut(s)	494, 618, 719, 746, 980, 1253, 1775, 1931
Hpy99I	CGWCG	1 cut(s)	29
HpyAV	CCTTC	8 cut(s)	328, 981, 1732, 1951, 1951, 2324, 2464, 3804
HpyCH4III	ACNGT	6 cut(s)	176, 754, 942, 1488, 1550, 2981
HpyCH4IV	ACGT	8 cut(s)	24, 151, 292, 1544, 2808, 2900, 2987, 3142
HpySE526I	ACGT	8 cut(s)	24, 151, 292, 1544, 2808, 2900, 2987, 3142
HspAI	GCGC	3 cut(s)	5, 1467, 3360
Kpn2I	TCCGGA	1 cut(s)	2911
KpnI	GGTACC	1 cut(s)	680
Ksp22I	TGATCA	1 cut(s)	2917
KspAI	GTTAAC	1 cut(s)	3502
LmnI	GCTCC	8 cut(s)	12, 613, 778, 1603, 2488, 2738, 3104, 3814
LweI	GCATC	7 cut(s)	305, 463, 1664, 1849, 2792, 3167, 3629
MaeII	ACGT	8 cut(s)	24, 151, 292, 1544, 2808, 2900, 2987, 3142
MaeIII	GTNAC	8 cut(s)	206, 409, 1342, 1362, 1919, 2320, 2804, 2996
MfeI	CAATTG	2 cut(s)	1056, 2726
MflI	RGATCY	6 cut(s)	1021, 1501, 2475, 2853, 3004, 3445
MhlI	GDGCHC	6 cut(s)	565, 1560, 2567, 2735, 3237, 3810
MlsI	TGGCCA	1 cut(s)	3099
MluNI	TGGCCA	1 cut(s)	3099
MlyI	GAGTC	3 cut(s)	491, 656, 1916
MmeI	TCCRAC	5 cut(s)	266, 1072, 2167, 2439, 2861
Mox20I	TGGCCA	1 cut(s)	3099
MroI	TCCGGA	1 cut(s)	2911
MroXI	GAANNNNTTC	3 cut(s)	362, 2690, 3048
MscI	TGGCCA	1 cut(s)	3099
MslI	CAYNNNNRTG	1 cut(s)	3455
Msp20I	TGGCCA	1 cut(s)	3099
MspA1I	CMGCKG	2 cut(s)	770, 3633
MspCI	CTTAAG	2 cut(s)	343, 1838
MspI	CCGG	3 cut(s)	1413, 2912, 3767
MspR9I	CCNGG	6 cut(s)	47, 621, 1221, 1414, 2580, 2960
MunI	CAATTG	2 cut(s)	1056, 2726
Mva1269I	GAATGC	1 cut(s)	109
MvaI	CCWGG	5 cut(s)	47, 621, 1221, 2580, 2960
MvnI	CGCG	2 cut(s)	15, 2448
NciI	CCSGG	1 cut(s)	1414
NcoI	CCATGG	2 cut(s)	3053, 3100
NdeI	CATATG	2 cut(s)	631, 3546
NlaIV	GGNNCC	7 cut(s)	187, 678, 1332, 1503, 2665, 2950, 3059
NmuCI	GTSAC	5 cut(s)	206, 409, 1342, 1919, 2996
NspI	RCATGY	6 cut(s)	342, 1075, 3298, 3393, 3557, 3784
PaeI	GCATGC	2 cut(s)	342, 3557
PaeR7I	CTCGAG	2 cut(s)	534, 3228
PaqCI	CACCTGC	1 cut(s)	1000
PceI	AGGCCT	1 cut(s)	1198
PciI	ACATGT	2 cut(s)	1071, 3294
PctI	GAATGC	1 cut(s)	109
PdmI	GAANNNNTTC	3 cut(s)	362, 2690, 3048
Pfl23II	CGTACG	1 cut(s)	16
PleI	GAGTC	3 cut(s)	491, 656, 1916
PpsI	GAGTC	3 cut(s)	491, 656, 1916
Ppu21I	YACGTR	2 cut(s)	293, 2988
PscI	ACATGT	2 cut(s)	1071, 3294
PshBI	ATTAAT	2 cut(s)	1686, 1695
PsiI	TTATAA	1 cut(s)	1295
Psp124BI	GAGCTC	1 cut(s)	3237
Psp6I	CCWGG	5 cut(s)	45, 619, 1219, 2578, 2958
PspFI	CCCAGC	2 cut(s)	922, 1610
PspGI	CCWGG	5 cut(s)	45, 619, 1219, 2578, 2958
PspLI	CGTACG	1 cut(s)	16
PspN4I	GGNNCC	7 cut(s)	187, 678, 1332, 1503, 2665, 2950, 3059
PspPI	GGNCC	5 cut(s)	1217, 2189, 2663, 3222, 3788
PstI	CTGCAG	1 cut(s)	1858
PstNI	CAGNNNCTG	1 cut(s)	3494
PsuI	RGATCY	6 cut(s)	1021, 1501, 2475, 2853, 3004, 3445
PvuII	CAGCTG	1 cut(s)	770
RsaI	GTAC	9 cut(s)	18, 268, 523, 678, 1547, 2651, 2983, 3172, 3840
RsaNI	GTAC	9 cut(s)	17, 267, 522, 677, 1546, 2650, 2982, 3171, 3839
RseI	CAYNNNNRTG	1 cut(s)	3455
SacI	GAGCTC	1 cut(s)	3237
Sau96I	GGNCC	5 cut(s)	1217, 2189, 2663, 3222, 3788
SchI	GAGTC	3 cut(s)	491, 656, 1916
ScrFI	CCNGG	6 cut(s)	47, 621, 1221, 1414, 2580, 2960
SduI	GDGCHC	6 cut(s)	565, 1560, 2567, 2735, 3237, 3810
SfaNI	GCATC	7 cut(s)	305, 463, 1664, 1849, 2792, 3167, 3629
SfcI	CTRYAG	5 cut(s)	1146, 1854, 2698, 2953, 3348
Sfr274I	CTCGAG	2 cut(s)	534, 3228
SinI	GGWCC	3 cut(s)	2189, 2663, 3788
SlaI	CTCGAG	2 cut(s)	534, 3228
SmiMI	CAYNNNNRTG	1 cut(s)	3455
SmlI	CTYRAG	5 cut(s)	343, 534, 1838, 3228, 3679
SmoI	CTYRAG	5 cut(s)	343, 534, 1838, 3228, 3679
SphI	GCATGC	2 cut(s)	342, 3557
SseBI	AGGCCT	1 cut(s)	1198
SsiI	CCGC	7 cut(s)	13, 144, 1041, 2234, 2356, 2448, 3633
SspI	AATATT	2 cut(s)	122, 1872
SstI	GAGCTC	1 cut(s)	3237
StuI	AGGCCT	1 cut(s)	1198
StyD4I	CCNGG	6 cut(s)	45, 619, 1219, 1412, 2578, 2958
StyI	CCWWGG	2 cut(s)	3053, 3100
TaaI	ACNGT	6 cut(s)	176, 754, 942, 1488, 1550, 2981
TaiI	ACGT	8 cut(s)	27, 154, 295, 1547, 2811, 2903, 2990, 3145
TaqI	TCGA	5 cut(s)	535, 1389, 1827, 1955, 3229
TatI	WGTACW	2 cut(s)	266, 2981
TseFI	GTSAC	5 cut(s)	206, 409, 1342, 1919, 2996
Tsp45I	GTSAC	5 cut(s)	206, 409, 1342, 1919, 2996
TspGWI	ACGGA	5 cut(s)	168, 635, 1166, 1361, 1990
Vha464I	CTTAAG	2 cut(s)	343, 1838
VneI	GTGCAC	1 cut(s)	2563
VpaK11BI	GGWCC	3 cut(s)	2189, 2663, 3788
VspI	ATTAAT	2 cut(s)	1686, 1695
XapI	RAATTY	7 cut(s)	529, 545, 568, 1079, 1792, 2571, 3246
XbaI	TCTAGA	1 cut(s)	3242
XceI	RCATGY	6 cut(s)	342, 1075, 3298, 3393, 3557, 3784
XhoI	CTCGAG	2 cut(s)	534, 3228
XmiI	GTMKAC	2 cut(s)	598, 692
XmnI	GAANNNNTTC	3 cut(s)	362, 2690, 3048

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.