Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0006478

Physical Location & Seq

Genomic Coordinates Reverse (-)

1064533 .. 1079801

Gene Structure 15269 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1009400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3870 bp

ATGGCCCTCCGCCGTCCGGGGCTTCTCCTCCCCCTCTCTTTCTTCCAGGTGTTATTTATTCTGGGCGTAGCATACGCTGCTCAGACCTCCGTGACGTCTAATACTACGGCGGCGGGGGAAAGACATTCGGAAGGGTATTGCGCAATGTATGGCATTTGCGGGCAACGTAGTGATGGAAAGTACATAAACTGCCCCTTCGGTACCCCATCCGTTAAGCCGGATGATTTACTTTCGTCCAAGGTCCAAAGTCTGTGTCCAACAATTACTGGAAATGTTTGTTGTACAGAAGCTCAATTTGACACACTACGGTCACAGGTCCAGCAAGCGATTCCCTTTCTTGTAGGTTGTCCGGCATGCTTAAGAAACTTTTTGAATTTGTTTTGTGAGCTTACCTGCTCTCCAAATCAGAGTTTGTTTATCAATGTGACTTCTGTAGCCAAGGTTAACAGAAACATGACTGTCAATGGGATTGATTATTATATAACTGATGACTTTGGTGAAGGGTTATTTAACTCCTGCAAGGATGTCAAGTTCGGTACCATGAATTCTCGAGCTATAGAATTTATTGGTGCTGGTGCTAAAAATTTTAAAGACTGGTTTGCTTTTATCGGCAGACAAGCACCTTCTAATGTGCCAGGTTCTCCATATGCCATTAGATTCCCTTCAAGTGTTACTGTGTCATCTGGGATGAAACCTATGAATGTATCTACTTATTCATGTGGTGATAATTCACTGGGCTGTTCCTGTGGTGATTGCCCTTCAGCTACAGTTTGTTCTAACACAGTTTCTCCGGTTTCCCGAAAAGGAGGTTCATGTTCAGTGAGAATTGGATCGATTAAGGCTAAATGTATTGACCTTGCTGTTGCTGTTCTATATATTGCACTGGTTTCTGTGTTTTTTGGGTGGGGTTTGTTTCATCGGACTAGAAAAACCAAGCCAGCTTCTGGGACAAAACCATGGTGGAATGTAATGGATGATGGCGAAGTTCATTCTAACAGGGAGAAGAATGAGAACCCTCCGATGCAGGTATTTGAAGATGCTTCTCACGTTAGAAATGATGTCCAACTTTCAATTGTGCAAGGATACATGTCAAAATTTTTCAGGAGATATGGAACATGGGTTGCTAGGAATCCAATTATAGTGTTGTGCTCATCATTGGCTGTAGTTCTTCTGCTTTGTTTGGGTCTTATCCGTTTCAAGGTGGAAACAAGGCCTGAAAAGCTGTGGGTAGGGCCTGGGAGTAAAGCAGCAGAAGAGAAGCAATTTTTTGATAGCCACCTAGCCCCTTTCTACAGAATTGAGCAGCTAATATTAGCAACAATTCCAGAGGGTAAGCTTGGTAACTCACCTAGTATTGTGACGGAGGACAATATTAAGTTACTTTTTGAGATACAGAAGAAGGTTGATGGAATTAAAGCAAATTATTCTGGATCCGCGGTATCTCTTGCTGATATTTGCATGAAGCCAATGGACAAAGATTGCGCCACTCAAAGTGTCCTACAGTACTTCAAAATGGATCCTGCAAATTATGATGGTTATGGTGGGGTTGAACACCTAAAGTATTGTTTTGAGCACTATTCATCTGCCGACACATGTATGAGTGCATTTAAAGGTCCTCTTGATCCTAGCACAGCATTAGGAGGCTTCTCTGGGAAAAACTACTCTGAGGCGTCAGCATTTCTTGTAACTTATCCTGTAAACAATGCTCGTAGTAATGATGGGAATGAAACTGAAAGGGCATTGGCATGGGAGAAAGCCTTTATTGAGCTTGCAAAGGATGAATTGTTGCAAATGGTGCAATCTAGAAACTTAACACTTTCTTTTTCATCGGAAAGCTCTGTTGAAGAAGAACTTAAAAGAGAAAGTACAGCAGATGCTATTACAATATTGATAAGCTATCTTGTGATGTTTGCTTATATATCTCTTACTTTGGGTGATTCACCTCGTTTATCATCATTTTACATTTCGTCTAAGGTATTGCTTGGTCTCTCTGGGGTTGTGCTTGTCATGCTGTCAGTTCTTGGATCAGTGGGATTTTTCAGTGTTATAGGTGTAAAATCTACCTTAATCATCATGGAAGTTATTCCATTTCTTGTTCTAGCTGTTGGGGTAGATAATATGTGTATACTGGTGAATGCTGTCAAACGGCAACCCTTGGAGTTGCCTTTAGAAGGGCGAATAAGCAATGCACTTGTGGAAGTTGGACCATCAATTACACTAGCTAGTCTATCAGAGGTCTTAGCGTTTGCAGTTGGAAGTTTTATTCCTATGCCAGCATGCCGCGTGTTCTCCATGTTTGCAGCACTTGCTGTTCTGCTGGACTTTCTCCTTCAAGTTACTGCATTTGTAGCTTTGATTGTTTTTGATTTTCGGAGAACTGAGGATAAGAGGGTTGATTGTTTCCCATGCATGAAAATTTCATCATATGCCAACTCTGACAAAGGTATTGATCAGAACAATCCTGGTTTATTGACTCGTTATATGAAGGAGATCCATGCTCCCATTCTCAGTCTTTGGGGAGTCAAGATGGTTGTCATTTCCATCTTTGTTGCATTTGCATTGGCTAGTATTGCATTATGCACCAGAATTCAGCCTGGTTTGGAACAAAAGATTGTTCTTCCCCGAGACTCCTATCTTCAGGGGTATTTCAATAATGTTTCGGAGTATCTCCGAATTGGACCCCCACTATACTTTGTTGTGAAGAACTTCAATTATAGTTCAGAATCAAGACATACGAACCAATTATGCTCAATTAGCCAGTGTGATTCAGATTCTCTTTTGAATGAGATTGCAAGAGCATCCTTAAATCCAGAATCAAGTTACATTGCTAAGCCTGCTGCTTCATGGCTTGATGATTTTCTTGTGTGGATATCTCCAGAAGCATTTGGGTGCTGTCGGAAGTTTACAGATGGGACTTACTGCCCTCCTGATGATCAGCCACCTTGCTGCTCTCCCAGTGATAGTTCCTGTAGCCTTGGTGGAGTGTGCAAAGACTGTACTACGTGCTTTCGTCACTCAGATCTTCACAATGACCGTCCCTCTACCACACAATTTAAAGAAAAACTTCCATGGTTCCTCAATGCTCTACCCTCTAATGATTGTGCTAAAGGTGGCCATGGAGCTTACACTAGCAGTGTGGAATTCAAAGGCAATGAAAGTGGCATTATTCAAGCATCATCATTTCGAACATATCATACTCCTCTCAACAAGCAGGTTGACTACGTAAATTCAATGAGAGCTGCTCGGGAGCTCAGTTCTAGACTTTCTGATTCTTTGAAGATTGAGATATTCCCATATTCAGTTTACTATATGTTCTTTGAGCAATACCTTGATATTTGGAGAACAGCACTGATCAACCTTTCTATAGCCATTGCTGCTGTGTTTATTGTTTGTTTAGCTATCACATGCAGTTTATGGAGTTCAGCTATTATTCTGTTGGTGTTGGCAATGATTTTAGTGGATCTCATGGGTGTGATGGCAATTCTGAACATCCAATTGAATGCAGTCTCTGTTGTGAATCTTGTAATGGCAGTGGGCATTTCGGTTGAGTTTTGTGTGCATATGACACATGCTTTCTCGGTTAGCACAGGAGATAAAGACCAGCGCACCAAGGAGGCTCTGGCTACAATGGGTGCCTCTGTATTCAGTGGAATCACATTAACAAAGTTAGTTGGGGTTATTGTACTTTGTTTCTCGAGGACGGAAATCTTTGTGGTGTATTATTTCCAAATGTACCTAGCATTGGTTCTTCTTGGTTTCTTACATGGGCTTGTGTTCTTGCCGGTCTTCTTGAGCGTGTTTGGTCCGCCTTCAAGACGCGTGCCTGTTGAGCAGAGACAAGATCAGACATCTGTTTCCCCACAACTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0003674 GO:0005488 GO:0005496 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005886 GO:0005887 GO:0006082 GO:0006464 GO:0006486 GO:0006629 GO:0006807 GO:0006810 GO:0006869 GO:0007034 GO:0007041 GO:0008150 GO:0008152 GO:0008202 GO:0008206 GO:0008289 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0010876 GO:0012505 GO:0015485 GO:0015850 GO:0015918 GO:0016020 GO:0016021 GO:0019538 GO:0019752 GO:0030301 GO:0031224 GO:0031226 GO:0031967 GO:0031975 GO:0032787 GO:0032934 GO:0033036 GO:0034645 GO:0036094 GO:0036211 GO:0042592 GO:0042632 GO:0043170 GO:0043178 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0048471 GO:0048878 GO:0051179 GO:0051234 GO:0055088 GO:0055092 GO:0065007 GO:0065008 GO:0070085 GO:0071702 GO:0071704 GO:0071944 GO:0097159 GO:1901135 GO:1901137 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901615

KEGG Pathways

Metabolic & Signaling

ko04142 ko04979 map04142 map04979

Pfam Domains

Protein Families

NPC1_N Patched

Protein Analysis

1290

Amino Acids

141.53

Weight (kDa)

6.76

Isoelectric Point (pI)

42.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NPC1_N	PF16414	45 - 274	3.7e-65	Niemann-Pick C1 N terminus
NPC1_MLD	PF22314	420 - 611	5.3e-37	NPC1, middle luminal domain
Patched	PF02460	634 - 787	1.8e-31	Patched SSD
Sterol-sensing	PF12349	655 - 805	2e-48	Sterol-sensing domain of SREBP cleavage-activation

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006478)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G42470
fragaria_vesca	FvH4_5g25290 FvH4_5g25290 FvH4_5g25290 FvH4_5g25290 FvH4_5g25290 FvH4_5g25290 FvH4_5g25290
malus_domestica	MD06G1009400.v1.1
prunus_persica	Prupe.5G011100_v2.0.a1 Prupe.5G011100_v2.0.a1 Prupe.5G011100_v2.0.a1 Prupe.5G011100_v2.0.a1
pyrus_communis	pycom06g00780 pycom16g25890
rosa_chinensis	RchiOBHm_Chr7g0215501
rosa_laevigata	RLG00000002657
rosa_multiflora	Rmu_sc0005928.1_g000005
rosa_roxburghii	Rroxscaffold_3G00244190
rosa_rugosa	Rorug07G0154100 Rorug07G0154200
rosa_samantha	Rh7AG290300 Rh7BG282300 Rh7CG310300 Rh7DG294100
rosa_wichuraiana	Rw7G024790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	98
Acc16I	TGCGCA	1 cut(s)	142
Acc36I	ACCTGC	3 cut(s)	401, 1015, 3202
Acc65I	GGTACC	2 cut(s)	200, 536
AccB1I	GGYRCC	3 cut(s)	200, 536, 3632
AccB7I	CCANNNNNTGG	1 cut(s)	944
AccI	GTMKAC	1 cut(s)	2125
AccII	CGCG	3 cut(s)	1436, 2283, 3819
AciI	CCGC	8 cut(s)	10, 110, 113, 159, 1434, 1436, 2281, 3806
AclWI	GGATC	9 cut(s)	838, 1425, 1438, 1511, 1524, 1616, 2032, 2485, 3468
AcoI	YGGCCR	1 cut(s)	3112
AcsI	RAATTY	9 cut(s)	373, 544, 560, 583, 1094, 2415, 2586, 3140, 3226
AcuI	CTGAAG	2 cut(s)	744, 2621
AcyI	GRCGYC	2 cut(s)	95, 1670
AfaI	GTAC	9 cut(s)	182, 202, 283, 538, 1505, 1867, 2998, 3684, 3734
AfiI	CCNNNNNNNGG	5 cut(s)	16, 944, 1198, 2171, 3742
AflII	CTTAAG	1 cut(s)	358
AflIII	ACRYGT	3 cut(s)	1086, 1592, 3817
AjnI	CCWGG	5 cut(s)	45, 634, 1234, 2461, 2593
AloI	GAACNNNNNNTCC	2 cut(s)	515, 547
Alw21I	GWGCWC	3 cut(s)	1151, 1575, 3252
Alw26I	GTCTC	4 cut(s)	1991, 2619, 3511, 3829
AlwI	GGATC	9 cut(s)	838, 1425, 1438, 1511, 1524, 1616, 2032, 2485, 3468
AlwNI	CAGNNNCTG	2 cut(s)	944, 3509
Ama87I	CYCGRG	4 cut(s)	549, 2622, 3243, 3694
AoxI	GGCC	4 cut(s)	3, 1211, 1232, 3112
ApeKI	GCWGC	8 cut(s)	77, 1247, 1303, 2300, 2837, 2946, 3239, 3374
ApoI	RAATTY	9 cut(s)	373, 544, 560, 583, 1094, 2415, 2586, 3140, 3226
ArsI	GACNNNNNNTTYG	2 cut(s)	238, 270
Asp700I	GAANNNNTTC	3 cut(s)	2082, 2705, 3063
Asp718I	GGTACC	2 cut(s)	200, 536
AspLEI	GCGC	3 cut(s)	143, 1484, 3606
AspS9I	GGNCC	8 cut(s)	4, 241, 316, 1232, 1613, 2204, 2678, 3803
AsuC2I	CCSGG	1 cut(s)	18
AsuHPI	GGTGA	7 cut(s)	509, 734, 761, 1338, 1932, 1946, 2143
AsuII	TTCGAA	1 cut(s)	3184
AvaI	CYCGRG	4 cut(s)	549, 2622, 3243, 3694
AvaII	GGWCC	6 cut(s)	241, 316, 1613, 2204, 2678, 3803
BalI	TGGCCA	1 cut(s)	3114
BamHI	GGATCC	2 cut(s)	1430, 1516
BanI	GGYRCC	3 cut(s)	200, 536, 3632
BanII	GRGCYC	1 cut(s)	3252
BbsI	GAAGAC	1 cut(s)	3778
Bbv12I	GWGCWC	3 cut(s)	1151, 1575, 3252
BbvI	GCAGC	8 cut(s)	64, 1259, 1315, 2312, 2824, 2933, 3226, 3361
BceAI	ACGGC	2 cut(s)	123, 2162
BciT130I	CCWGG	5 cut(s)	47, 636, 1236, 2463, 2595
BciVI	GTATCC	1 cut(s)	1076
BclI	TGATCA	3 cut(s)	2449, 2932, 3351
BcnI	CCSGG	1 cut(s)	18
BcoDI	GTCTC	4 cut(s)	1991, 2619, 3511, 3829
BfmI	CTRYAG	8 cut(s)	432, 555, 765, 1161, 1291, 1499, 2968, 3363
BfrI	CTTAAG	1 cut(s)	358
BfuAI	ACCTGC	3 cut(s)	401, 1015, 3202
BfuI	GTATCC	1 cut(s)	1076
BglII	AGATCT	1 cut(s)	3019
BlpI	GCTNAGC	1 cut(s)	2829
BmcAI	AGTACT	1 cut(s)	1505
Bme1390I	CCNGG	6 cut(s)	18, 47, 636, 1236, 2463, 2595
Bme18I	GGWCC	6 cut(s)	241, 316, 1613, 2204, 2678, 3803
BmeT110I	CYCGRG	4 cut(s)	549, 2622, 3243, 3694
BmgT120I	GGNCC	8 cut(s)	4, 241, 316, 1232, 1613, 2204, 2678, 3803
BmiI	GGNNCC	7 cut(s)	202, 538, 1432, 1518, 2680, 3074, 3634
BmrFI	CCNGG	6 cut(s)	18, 47, 636, 1236, 2463, 2595
BmrI	ACTGGG	2 cut(s)	743, 2949
BmsI	GCATC	5 cut(s)	1011, 1027, 1864, 2807, 3182
BmuI	ACTGGG	2 cut(s)	743, 2949
BpiI	GAAGAC	1 cut(s)	3778
BplI	GAGNNNNNCTC	2 cut(s)	3226, 3258
BpmI	CTGGAG	1 cut(s)	2859
Bpu1102I	GCTNAGC	1 cut(s)	2829
Bpu14I	TTCGAA	1 cut(s)	3184
BpuEI	CTTGAG	1 cut(s)	3811
BpuMI	CCSGG	1 cut(s)	18
Bsa29I	ATCGAT	1 cut(s)	833
BsaAI	YACGTR	2 cut(s)	3003, 3223
BsaHI	GRCGYC	2 cut(s)	95, 1670
BsaI	GGTCTC	1 cut(s)	1991
BsaWI	WCCGGW	1 cut(s)	790
BsaXI	ACNNNNNCTCC	4 cut(s)	382, 412, 772, 802
Bsc4I	CCNNNNNNNGG	5 cut(s)	16, 944, 1198, 2171, 3742
Bse118I	RCCGGY	1 cut(s)	3781
Bse1I	ACTGG	7 cut(s)	271, 599, 738, 888, 2133, 2758, 2955
Bse3DI	GCAATG	6 cut(s)	150, 2191, 2823, 3157, 3369, 3453
BseBI	CCWGG	5 cut(s)	47, 636, 1236, 2463, 2595
BseCI	ATCGAT	1 cut(s)	833
BseGI	GGATG	8 cut(s)	206, 226, 529, 693, 979, 1783, 2798, 3489
BseLI	CCNNNNNNNGG	5 cut(s)	16, 944, 1198, 2171, 3742
BseMI	GCAATG	6 cut(s)	150, 2191, 2823, 3157, 3369, 3453
BseMII	CTCAG	6 cut(s)	95, 1656, 2370, 2521, 3030, 3265
BseNI	ACTGG	7 cut(s)	271, 599, 738, 888, 2133, 2758, 2955
BseRI	GAGGAG	2 cut(s)	17, 3189
BseXI	GCAGC	8 cut(s)	64, 1259, 1315, 2312, 2824, 2933, 3226, 3361
Bsh1236I	CGCG	3 cut(s)	1436, 2283, 3819
BshFI	GGCC	4 cut(s)	5, 1213, 1234, 3114
BshNI	GGYRCC	3 cut(s)	200, 536, 3632
BshVI	ATCGAT	1 cut(s)	833
BsiHKAI	GWGCWC	3 cut(s)	1151, 1575, 3252
BsiHKCI	CYCGRG	4 cut(s)	549, 2622, 3243, 3694
BsiSI	CCGG	5 cut(s)	17, 218, 350, 791, 3782
BslFI	GGGAC	3 cut(s)	961, 2926, 3021
BslI	CCNNNNNNNGG	5 cut(s)	16, 944, 1198, 2171, 3742
BsmAI	GTCTC	4 cut(s)	1991, 2619, 3511, 3829
BsmFI	GGGAC	3 cut(s)	961, 2926, 3021
BsmI	GAATGC	2 cut(s)	2140, 3505
BsnI	GGCC	4 cut(s)	5, 1213, 1234, 3114
Bso31I	GGTCTC	1 cut(s)	1991
BsoBI	CYCGRG	4 cut(s)	549, 2622, 3243, 3694
Bsp119I	TTCGAA	1 cut(s)	3184
Bsp1286I	GDGCHC	3 cut(s)	1151, 1575, 3252
Bsp1407I	TGTACA	1 cut(s)	281
Bsp1720I	GCTNAGC	1 cut(s)	2829
Bsp19I	CCATGG	3 cut(s)	956, 3068, 3115
BspACI	CCGC	8 cut(s)	10, 110, 113, 159, 1434, 1436, 2281, 3806
BspANI	GGCC	4 cut(s)	5, 1213, 1234, 3114
BspCNI	CTCAG	6 cut(s)	94, 1657, 2371, 2520, 3029, 3264
BspDI	ATCGAT	1 cut(s)	833
BspFNI	CGCG	3 cut(s)	1436, 2283, 3819
BspLI	GGNNCC	7 cut(s)	202, 538, 1432, 1518, 2680, 3074, 3634
BspMI	ACCTGC	3 cut(s)	401, 1015, 3202
BspPI	GGATC	9 cut(s)	838, 1425, 1438, 1511, 1524, 1616, 2032, 2485, 3468
BspT104I	TTCGAA	1 cut(s)	3184
BspT107I	GGYRCC	3 cut(s)	200, 536, 3632
BspTI	CTTAAG	1 cut(s)	358
BspTNI	GGTCTC	1 cut(s)	1991
BsrDI	GCAATG	6 cut(s)	150, 2191, 2823, 3157, 3369, 3453
BsrFI	RCCGGY	1 cut(s)	3781
BsrGI	TGTACA	1 cut(s)	281
BsrI	ACTGG	7 cut(s)	271, 599, 738, 888, 2133, 2758, 2955
BssAI	RCCGGY	1 cut(s)	3781
BssNAI	GTATAC	1 cut(s)	2126
BssNI	GRCGYC	2 cut(s)	95, 1670
BssT1I	CCWWGG	8 cut(s)	237, 438, 956, 2154, 2974, 3068, 3115, 3609
Bst1107I	GTATAC	1 cut(s)	2126
Bst2UI	CCWGG	5 cut(s)	47, 636, 1236, 2463, 2595
Bst4CI	ACNGT	8 cut(s)	309, 460, 676, 769, 784, 1503, 2996, 3035
Bst6I	CTCTTC	1 cut(s)	1248
BstACI	GRCGYC	2 cut(s)	95, 1670
BstAFI	CTTAAG	1 cut(s)	358
BstAPI	GCANNNNNTGC	3 cut(s)	77, 1795, 2306
BstAUI	TGTACA	1 cut(s)	281
BstBAI	YACGTR	2 cut(s)	3003, 3223
BstBI	TTCGAA	1 cut(s)	3184
BstC8I	GCNNGC	9 cut(s)	161, 324, 355, 939, 1770, 2274, 2278, 2835, 3821
BstDEI	CTNAG	9 cut(s)	81, 1665, 1971, 2239, 2379, 2507, 2829, 3016, 3251
BstDSI	CCRYGG	4 cut(s)	956, 1434, 3068, 3115
BstENI	CCTNNNNNAGG	1 cut(s)	2169
BstF5I	GGATG	8 cut(s)	206, 226, 529, 693, 979, 1783, 2798, 3489
BstFNI	CGCG	3 cut(s)	1436, 2283, 3819
BstHHI	GCGC	3 cut(s)	143, 1484, 3606
BstMAI	GTCTC	4 cut(s)	1991, 2619, 3511, 3829
BstNI	CCWGG	5 cut(s)	47, 636, 1236, 2463, 2595
BstNSI	RCATGY	6 cut(s)	357, 1090, 1596, 2280, 3408, 3572
BstSCI	CCNGG	6 cut(s)	16, 45, 634, 1234, 2461, 2593
BstSFI	CTRYAG	8 cut(s)	432, 555, 765, 1161, 1291, 1499, 2968, 3363
BstSNI	TACGTA	1 cut(s)	3223
BstUI	CGCG	3 cut(s)	1436, 2283, 3819
BstV1I	GCAGC	8 cut(s)	64, 1259, 1315, 2312, 2824, 2933, 3226, 3361
BstV2I	GAAGAC	1 cut(s)	3778
BstX2I	RGATCY	5 cut(s)	1430, 1516, 2490, 3019, 3460
BstYI	RGATCY	5 cut(s)	1430, 1516, 2490, 3019, 3460
BstZ17I	GTATAC	1 cut(s)	2126
Bsu15I	ATCGAT	1 cut(s)	833
BsuI	GTATCC	1 cut(s)	1076
BsuRI	GGCC	4 cut(s)	5, 1213, 1234, 3114
BsuTUI	ATCGAT	1 cut(s)	833
BtgI	CCRYGG	4 cut(s)	956, 1434, 3068, 3115
BtsCI	GGATG	8 cut(s)	206, 226, 529, 693, 979, 1783, 2798, 3489
BtsI	GCAGTG	2 cut(s)	3139, 3537
BveI	ACCTGC	3 cut(s)	401, 1015, 3202
Cac8I	GCNNGC	9 cut(s)	161, 324, 355, 939, 1770, 2274, 2278, 2835, 3821
CaiI	CAGNNNCTG	2 cut(s)	944, 3509
CfoI	GCGC	3 cut(s)	143, 1484, 3606
Cfr10I	RCCGGY	1 cut(s)	3781
Cfr13I	GGNCC	8 cut(s)	4, 241, 316, 1232, 1613, 2204, 2678, 3803
Cfr42I	CCGCGG	1 cut(s)	1437
ClaI	ATCGAT	1 cut(s)	833
CseI	GACGC	2 cut(s)	1659, 3825
Csp6I	GTAC	9 cut(s)	181, 201, 282, 537, 1504, 1866, 2997, 3683, 3733
CspCI	CAANNNNNGTGG	2 cut(s)	3139, 3174
CviQI	GTAC	9 cut(s)	181, 201, 282, 537, 1504, 1866, 2997, 3683, 3733
DdeI	CTNAG	9 cut(s)	81, 1665, 1971, 2239, 2379, 2507, 2829, 3016, 3251
DraI	TTTAAA	3 cut(s)	589, 1609, 3055
EaeI	YGGCCR	1 cut(s)	3112
Eam1104I	CTCTTC	1 cut(s)	1248
EarI	CTCTTC	1 cut(s)	1248
EciI	GGCGGA	1 cut(s)	3795
Ecl136II	GAGCTC	1 cut(s)	3250
Eco105I	TACGTA	1 cut(s)	3223
Eco130I	CCWWGG	8 cut(s)	237, 438, 956, 2154, 2974, 3068, 3115, 3609
Eco147I	AGGCCT	1 cut(s)	1213
Eco24I	GRGCYC	1 cut(s)	3252
Eco31I	GGTCTC	1 cut(s)	1991
Eco32I	GATATC	1 cut(s)	2871
Eco47I	GGWCC	6 cut(s)	241, 316, 1613, 2204, 2678, 3803
Eco53kI	GAGCTC	1 cut(s)	3250
Eco57I	CTGAAG	2 cut(s)	744, 2621
Eco88I	CYCGRG	4 cut(s)	549, 2622, 3243, 3694
EcoICRI	GAGCTC	1 cut(s)	3250
EcoNI	CCTNNNNNAGG	1 cut(s)	2169
EcoO109I	RGGNCCY	2 cut(s)	1232, 1613
EcoRI	GAATTC	3 cut(s)	544, 2586, 3140
EcoRII	CCWGG	5 cut(s)	45, 634, 1234, 2461, 2593
EcoRV	GATATC	1 cut(s)	2871
EcoT14I	CCWWGG	8 cut(s)	237, 438, 956, 2154, 2974, 3068, 3115, 3609
EcoT22I	ATGCAT	1 cut(s)	2411
EcoT38I	GRGCYC	1 cut(s)	3252
ErhI	CCWWGG	8 cut(s)	237, 438, 956, 2154, 2974, 3068, 3115, 3609
FalI	AAGNNNNNCTT	6 cut(s)	1602, 1634, 1836, 1868, 3048, 3080
FaqI	GGGAC	3 cut(s)	961, 2926, 3021
FauI	CCCGC	2 cut(s)	106, 152
FauNDI	CATATG	3 cut(s)	646, 2425, 3561
FbaI	TGATCA	3 cut(s)	2449, 2932, 3351
FblI	GTMKAC	1 cut(s)	2125
FokI	GGATG	8 cut(s)	193, 233, 536, 700, 986, 1790, 2785, 3476
FriOI	GRGCYC	1 cut(s)	3252
FspI	TGCGCA	1 cut(s)	142
GlaI	GCGC	3 cut(s)	142, 1483, 3605
GsuI	CTGGAG	1 cut(s)	2859
HaeIII	GGCC	4 cut(s)	5, 1213, 1234, 3114
HapII	CCGG	5 cut(s)	17, 218, 350, 791, 3782
HgaI	GACGC	2 cut(s)	1659, 3825
HhaI	GCGC	3 cut(s)	143, 1484, 3606
Hin1I	GRCGYC	2 cut(s)	95, 1670
Hin6I	GCGC	3 cut(s)	141, 1482, 3604
HinP1I	GCGC	3 cut(s)	141, 1482, 3604
HincII	GTYRAC	2 cut(s)	445, 3217
HindII	GTYRAC	2 cut(s)	445, 3217
HindIII	AAGCTT	1 cut(s)	1334
HpaI	GTTAAC	1 cut(s)	445
HpaII	CCGG	5 cut(s)	17, 218, 350, 791, 3782
HphI	GGTGA	7 cut(s)	509, 734, 761, 1338, 1932, 1946, 2143
Hpy166II	GTNNAC	6 cut(s)	445, 1699, 2126, 2904, 3217, 3304
Hpy8I	GTNNAC	6 cut(s)	445, 1699, 2126, 2904, 3217, 3304
HpyCH4III	ACNGT	8 cut(s)	309, 460, 676, 769, 784, 1503, 2996, 3035
HpyCH4IV	ACGT	5 cut(s)	95, 166, 1047, 3002, 3222
HpyF3I	CTNAG	9 cut(s)	81, 1665, 1971, 2239, 2379, 2507, 2829, 3016, 3251
HpySE526I	ACGT	5 cut(s)	95, 166, 1047, 3002, 3222
Hsp92I	GRCGYC	2 cut(s)	95, 1670
HspAI	GCGC	3 cut(s)	141, 1482, 3604
KpnI	GGTACC	2 cut(s)	204, 540
Ksp22I	TGATCA	3 cut(s)	2449, 2932, 3351
KspAI	GTTAAC	1 cut(s)	445
KspI	CCGCGG	1 cut(s)	1437
LmnI	GCTCC	3 cut(s)	2503, 3119, 3247
Lsp1109I	GCAGC	8 cut(s)	64, 1259, 1315, 2312, 2824, 2933, 3226, 3361
LweI	GCATC	5 cut(s)	1011, 1027, 1864, 2807, 3182
MaeII	ACGT	5 cut(s)	95, 166, 1047, 3002, 3222
MfeI	CAATTG	2 cut(s)	1071, 3494
MflI	RGATCY	5 cut(s)	1430, 1516, 2490, 3019, 3460
MhlI	GDGCHC	3 cut(s)	1151, 1575, 3252
MlsI	TGGCCA	1 cut(s)	3114
MluI	ACGCGT	1 cut(s)	3817
MluNI	TGGCCA	1 cut(s)	3114
MlyI	GAGTC	3 cut(s)	2467, 2529, 2621
MmeI	TCCRAC	5 cut(s)	281, 1087, 2182, 2233, 2876
Mox20I	TGGCCA	1 cut(s)	3114
Mph1103I	ATGCAT	1 cut(s)	2411
MroXI	GAANNNNTTC	3 cut(s)	2082, 2705, 3063
MscI	TGGCCA	1 cut(s)	3114
MslI	CAYNNNNRTG	5 cut(s)	1595, 1744, 2887, 3470, 3865
Msp20I	TGGCCA	1 cut(s)	3114
MspA1I	CMGCKG	1 cut(s)	1436
MspCI	CTTAAG	1 cut(s)	358
MspI	CCGG	5 cut(s)	17, 218, 350, 791, 3782
MspR9I	CCNGG	6 cut(s)	18, 47, 636, 1236, 2463, 2595
MunI	CAATTG	2 cut(s)	1071, 3494
Mva1269I	GAATGC	2 cut(s)	2140, 3505
MvaI	CCWGG	5 cut(s)	47, 636, 1236, 2463, 2595
MvnI	CGCG	3 cut(s)	1436, 2283, 3819
NciI	CCSGG	1 cut(s)	18
NcoI	CCATGG	3 cut(s)	956, 3068, 3115
NdeI	CATATG	3 cut(s)	646, 2425, 3561
NlaIV	GGNNCC	7 cut(s)	202, 538, 1432, 1518, 2680, 3074, 3634
NmuCI	GTSAC	5 cut(s)	91, 309, 424, 1357, 3011
NsbI	TGCGCA	1 cut(s)	142
NsiI	ATGCAT	1 cut(s)	2411
NspI	RCATGY	6 cut(s)	357, 1090, 1596, 2280, 3408, 3572
NspV	TTCGAA	1 cut(s)	3184
PaeI	GCATGC	2 cut(s)	357, 2280
PaeR7I	CTCGAG	2 cut(s)	549, 3694
PceI	AGGCCT	1 cut(s)	1213
PciI	ACATGT	2 cut(s)	1086, 1592
PctI	GAATGC	2 cut(s)	2140, 3505
PdmI	GAANNNNTTC	3 cut(s)	2082, 2705, 3063
PflMI	CCANNNNNTGG	1 cut(s)	944
PleI	GAGTC	3 cut(s)	2467, 2528, 2621
PpsI	GAGTC	3 cut(s)	2467, 2528, 2621
Ppu21I	YACGTR	2 cut(s)	3003, 3223
PpuMI	RGGWCCY	1 cut(s)	1613
PscI	ACATGT	2 cut(s)	1086, 1592
Psp124BI	GAGCTC	1 cut(s)	3252
Psp5II	RGGWCCY	1 cut(s)	1613
Psp6I	CCWGG	5 cut(s)	45, 634, 1234, 2461, 2593
PspGI	CCWGG	5 cut(s)	45, 634, 1234, 2461, 2593
PspN4I	GGNNCC	7 cut(s)	202, 538, 1432, 1518, 2680, 3074, 3634
PspPI	GGNCC	8 cut(s)	4, 241, 316, 1232, 1613, 2204, 2678, 3803
PspPPI	RGGWCCY	1 cut(s)	1613
PsrI	GAACNNNNNNTAC	2 cut(s)	3178, 3210
PstNI	CAGNNNCTG	2 cut(s)	944, 3509
PsuI	RGATCY	5 cut(s)	1430, 1516, 2490, 3019, 3460
RsaI	GTAC	9 cut(s)	182, 202, 283, 538, 1505, 1867, 2998, 3684, 3734
RsaNI	GTAC	9 cut(s)	181, 201, 282, 537, 1504, 1866, 2997, 3683, 3733
RseI	CAYNNNNRTG	5 cut(s)	1595, 1744, 2887, 3470, 3865
SacI	GAGCTC	1 cut(s)	3252
SacII	CCGCGG	1 cut(s)	1437
Sau96I	GGNCC	8 cut(s)	4, 241, 316, 1232, 1613, 2204, 2678, 3803
ScaI	AGTACT	1 cut(s)	1505
SchI	GAGTC	3 cut(s)	2467, 2529, 2621
ScrFI	CCNGG	6 cut(s)	18, 47, 636, 1236, 2463, 2595
SduI	GDGCHC	3 cut(s)	1151, 1575, 3252
SfaNI	GCATC	5 cut(s)	1011, 1027, 1864, 2807, 3182
SfcI	CTRYAG	8 cut(s)	432, 555, 765, 1161, 1291, 1499, 2968, 3363
Sfr274I	CTCGAG	2 cut(s)	549, 3694
Sfr303I	CCGCGG	1 cut(s)	1437
SfuI	TTCGAA	1 cut(s)	3184
SgrBI	CCGCGG	1 cut(s)	1437
SinI	GGWCC	6 cut(s)	241, 316, 1613, 2204, 2678, 3803
SlaI	CTCGAG	2 cut(s)	549, 3694
SmiMI	CAYNNNNRTG	5 cut(s)	1595, 1744, 2887, 3470, 3865
SmlI	CTYRAG	4 cut(s)	358, 549, 3694, 3790
SmoI	CTYRAG	4 cut(s)	358, 549, 3694, 3790
SnaBI	TACGTA	1 cut(s)	3223
SphI	GCATGC	2 cut(s)	357, 2280
SseBI	AGGCCT	1 cut(s)	1213
SsiI	CCGC	8 cut(s)	10, 110, 113, 159, 1434, 1436, 2281, 3806
SspI	AATATT	3 cut(s)	1311, 1372, 1887
SstI	GAGCTC	1 cut(s)	3252
StuI	AGGCCT	1 cut(s)	1213
StyD4I	CCNGG	6 cut(s)	16, 45, 634, 1234, 2461, 2593
StyI	CCWWGG	8 cut(s)	237, 438, 956, 2154, 2974, 3068, 3115, 3609
TaaI	ACNGT	8 cut(s)	309, 460, 676, 769, 784, 1503, 2996, 3035
TaiI	ACGT	5 cut(s)	98, 169, 1050, 3005, 3225
TaqI	TCGA	4 cut(s)	550, 833, 3184, 3695
TatI	WGTACW	6 cut(s)	180, 281, 1503, 1865, 2996, 3682
TauI	GCSGC	2 cut(s)	113, 2283
TseFI	GTSAC	5 cut(s)	91, 309, 424, 1357, 3011
TseI	GCWGC	8 cut(s)	77, 1247, 1303, 2300, 2837, 2946, 3239, 3374
Tsp45I	GTSAC	5 cut(s)	91, 309, 424, 1357, 3011
TspGWI	ACGGA	5 cut(s)	79, 199, 1181, 1376, 3716
Van91I	CCANNNNNTGG	1 cut(s)	944
Vha464I	CTTAAG	1 cut(s)	358
VpaK11BI	GGWCC	6 cut(s)	241, 316, 1613, 2204, 2678, 3803
XagI	CCTNNNNNAGG	1 cut(s)	2169
XapI	RAATTY	9 cut(s)	373, 544, 560, 583, 1094, 2415, 2586, 3140, 3226
XbaI	TCTAGA	2 cut(s)	1802, 3257
XceI	RCATGY	6 cut(s)	357, 1090, 1596, 2280, 3408, 3572
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3616
XhoI	CTCGAG	2 cut(s)	549, 3694
XmiI	GTMKAC	1 cut(s)	2125
XmnI	GAANNNNTTC	3 cut(s)	2082, 2705, 3063
ZraI	GACGTC	1 cut(s)	96
ZrmI	AGTACT	1 cut(s)	1505
Zsp2I	ATGCAT	1 cut(s)	2411

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.