Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7C

Orthogroup ID

OG0006478

Physical Location & Seq

Genomic Coordinates Reverse (-)

32778957 .. 32794776

Gene Structure 15820 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7CG310300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3810 bp

ATGGAATCATTTTTATGCTCATATGTTCAGGTGTTATGGATTCTGCCTCTGGCATTTGCTCAGAGTTCTATAAAGTCCAGTGTTACCAGGCATTCTGAAGAATATTGTGCTATGTATGACATTTGTGGTAAACGTACTGATGGAAAAGTATTGAACTGTCCGTACGGTTCCCCATCTGTAAAGCCAAGTGACTTACTTTCATCTAAGATCCAAAGTTTGTGTCCAACAATTACTGGAAATGTCTGTTGTACAGCAGCCCAATTTGACACATTACGGACATCGGTTCAGCCAGCAATTCCCTTTCTTGTAGGATGTCCAGCATGCTTAAGAAACTTTTTGAACCTCTTCTGTGAGCTTACCTGCTCTCCAAATCAGAGTTTATTTATCAATGTGACTTCTGTAAACAAGGTAAATAACAATATGACCGTTGGAGGGATTGATTATTACATAAGTGATACTTTTGGTGAGGGGTTGTATGACTCCTGCAAGGATGTCAAGTTTGGTACCATGAATTCTAGAGCTATGGAATTCATTGGTGCTGGTGCTCAAAATTTTAAAGAGTGGTTTACTTTTATCGGTAGACAAGCAGCTCCTACTCTGCCAGGTTCACCATATGCCATTAGATTCCGGTCAAATGCTACTGACTCATCCGAGATGATACCTATGAATGTATCTACTTATTCTTGTGGTGATAATTCACTGGGCTGTTCCTGTGGTGATTGCCCGTCATCCACAGTTTGTTCTAACACAGCTGTTCCAGTTTCCCCCAAAAAAGGGTCATGTACAGTGAAAATTGGATCTATTAAGGCAAAATGTATTGACCTTGCTGTCGCCGTTCTATATATCGTACTGGTTTCTGTGTTTTTTGGGTGGGGTTTGTTTAATCGGACTAGGAAGCAAACCCCGGCTTCTAGGAAAAAACCGTTGTGGAATAATGTAATGGATGATGGCGAAGTTCAGTCTATAAGCAGGGAGAAGATCGAGAATCCTCCAATGCAGGTTCTTGAAGATCCTCCCCACATGAGAAGCAGTGTCCAACTTTCAATTGTGCAAGGATACATGTCAAAATTTTATAGGGGATATGGGAAGTGGGTTGCTAGAAATCCGATCACAGTGTTGGGTTCATCGTCTGCTATAGTTCTCCTACTTTGTCTTGGTCTTATCCGGTTCAAGGTGGAAACAAGGCCTGAGAAGCTATGGGTAGGGCCAGGGAGTAAAGCAGCACAGGAGAAGCAATTTTTTGATAGTCACCTAGCCCCTTTTTACAGAATTGAGCAGCTTATATTAGCAACAATACCAGATGCCAAGCATGGTAAGTCACCTAGTATTGTGACGGAGGACAACATCAAGTTACTTTTTGAGATACAAAAAAAGGTTGACGGAATTAAAGCAAATTATTCTGGGTCAATGATATCTCTTGCTGATATCTGCATGAAGCCACTAGACCAAGATTGCGCCAGTCAAAGTGTCATACAGTATTTCAAATTGGATCCTGAAAATTTTGACAATTATGGTGGGATTGAACACCTAACGTATTGTTTTGAGCACTATTCCTCTGCTGACAAATGTATGAGTGCATATAAAGCTCCTCTTGATCCCAGCACTGCTTTAGGTGGTTTCTCTGGGAAAAACTATTCCGAGGCATCAGCATTTATAATAACTTATCCTGTAAACAATGCTATTAATAAAGAGGGGAATGAAACTGAAAGAGCAGTGGCATGGGAGAAGGCCTTTATTCAGTTGGCAAAGGCTGAGCTGTTGCAAATGGTGCAATCTAAAAACTTAACACTATCTTTCTCATCGGAGAGTTCCGTTGAAGAAGAACTTAAGAGAGAAAGCACTGCAGATGCTATTACAATATTGATAAGCTATCTTGTGATGTTCGCTTATATTTCTCTTACTTTGGGTGACTCTCCTCGTTTGTCTTCCTTTTACATTTCATCAAAGGTATTGCTAGGTCTCTCTGGAGTTGTTCTTGTCATGCTCTCCGTTCTTGGATCAGTGGGATTTTTCAGTGCCATTGGAGTTAAATCTACCCTAATTATTATGGAAGTCATCCCATTTCTTGTTCTAGCTGTCGGGGTAGATAATATGTGTATACTGGTTCATGCTGTCAAACGGCAACCATTGACGATGCCTTTAGAAGGGCGAATAAGCAATGCACTTGTGGAAGTTGGACCATCCATAACTCTTGCTAGTCTTTCAGAAGTTTTAGCATTTGCGGTTGGCAGCTTTATTCCTATGCCAGCTTGTCGTGTGTTCTCCATGTTTGCTGCACTAGCTGTTCTTTTGGACTTCCTCCTTCAAGTTACTGCTTTTGTAGCTCTGATTGTTCTTGATTTTCGCCGAACTGAGGATAATAGGGTTGATTGTTTCCCTTGTATGAAAGTTTCATCATATACCAACTCTGATAAAGGCATTGAAGTAAGAAAACCAGGTTTGCTGGCACGCTATATGAAGGGAATCCATGCTCCCATTCTAAGTGTTTGGGTTGTTAAGATAGTTGTCATTTCCATTTTTGTTGCGTTTTCACTGGCTAGCATTGCGTTGTGCACCAGAATTCAGCCTGGTTTGGAACAAAAAATTGTTCTTCCCCAAGATTCCTATCTTCAGGGTTATTTCAATAATATATCAGAGTATCTCCGAATTGGACCCCCACTATATTTTGTTGTGAAGAACTACAACTATAGTTCAGAATCAAGACATACAAACCAATTGTGCTCCATTAGCCAATGTGATTCAGATTCTCTTTTGAATGAGATTGCAAGAGCATCCTTAATTCCAGAATCAAGTTACATTGCTAAGCCTGCTGCTTCATGGCTTGATGATTTTCTTGTGTGGATTTCCCCAGAAGCTTTTGGGTGCTGTCGGAAGTTTACAAATGCAACTTATTGTCCTCCTGATGATCAGCCTCCTTGCTGTTTTTCCTCTGATGGTTCCTGTAGCCTGGGTGGAGTGTGCAAAGATTGTACCACGTGTTTTCGTCACTCAGATCTGCATAATGGTCGTCCATCGACCACACAATTTGAAGAAAAACTTCCATGGTTCCTCGATGCTCTGCCCTCTTCAGATTGTGCTAAAGGTGGCCATGGAGCTTACACTAGCAGTGTGGAATTGAAAGGCTATGAAAGTGGCATTATTCAAGCGTCATCATTTCGTACATATCATACACCTCTCAACAAACAGATTGACTATGTAAACTCAATGAGGGCTGCTCGAGAGCTCTGTTCTAGAGTTTCTGATTCTTTGAAGATCGAGATATTCCCATATTCAGTGTTTTACATGTTCTTTGAGCAGTACCTCGATATTTGGAAAACAGCACTTATGAGCCTTTCTATAGCTATTGGTGCTGTATTTATTGTTTGCCTAGTCATCACATGCAGCTTGTGGAGTTCAGCAATTATCCTTTTGGTGTTGGCAATGATTGTTGTAGATCTCATGGGAGTGATGGCAATTCTTCACATTCAATTAAATGCAGTCTCTGTTGTTAACCTTGTTATGGCAGTGGGCATTTCTGTTGAGTTTTGTGTGCATATGACACATGCTTTCTCGGTTAGCACAGGAGATAGAGATCAACGAATAAAGGAGGCTCTGGGTACAATGGGTGCATCTGTCTTCAGTGGAATCACACTTACGAAGTTAGTTGGGGTTATTGTACTTTGTTTCTCAAGAACAGAAGTCTTTGTGGTGTATTACTTTCAAATGTATCTAGCATTAGTTCTTCTTGGTTTCTTACATGGACTGGTATTTTTGCCGGTGGTGTTGAGCATTTTTGGTCCCCCTTCGAGATGTCTGCCCAGGGAGCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0003674 GO:0005488 GO:0005496 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005886 GO:0005887 GO:0006082 GO:0006464 GO:0006486 GO:0006629 GO:0006807 GO:0006810 GO:0006869 GO:0007034 GO:0007041 GO:0008150 GO:0008152 GO:0008202 GO:0008206 GO:0008289 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0010876 GO:0012505 GO:0015485 GO:0015850 GO:0015918 GO:0016020 GO:0016021 GO:0019538 GO:0019752 GO:0030301 GO:0031224 GO:0031226 GO:0031967 GO:0031975 GO:0032787 GO:0032934 GO:0033036 GO:0034645 GO:0036094 GO:0036211 GO:0042592 GO:0042632 GO:0043170 GO:0043178 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0048471 GO:0048878 GO:0051179 GO:0051234 GO:0055088 GO:0055092 GO:0065007 GO:0065008 GO:0070085 GO:0071702 GO:0071704 GO:0071944 GO:0097159 GO:1901135 GO:1901137 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901615

KEGG Pathways

Metabolic & Signaling

ko04142 ko04979 map04142 map04979

Pfam Domains

Protein Families

NPC1_N Patched

Protein Analysis

1269

Amino Acids

139.48

Weight (kDa)

6.93

Isoelectric Point (pI)

43.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NPC1_N	PF16414	34 - 263	2e-66	Niemann-Pick C1 N terminus
NPC1_MLD	PF22314	411 - 602	2.1e-38	NPC1, middle luminal domain
Patched	PF02460	625 - 778	1.5e-31	Patched SSD
Sterol-sensing	PF12349	646 - 796	9.5e-49	Sterol-sensing domain of SREBP cleavage-activation

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0006478)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G42470
fragaria_vesca	FvH4_5g25290 FvH4_5g25290 FvH4_5g25290 FvH4_5g25290 FvH4_5g25290 FvH4_5g25290 FvH4_5g25290
malus_domestica	MD06G1009400.v1.1
prunus_persica	Prupe.5G011100_v2.0.a1 Prupe.5G011100_v2.0.a1 Prupe.5G011100_v2.0.a1 Prupe.5G011100_v2.0.a1
pyrus_communis	pycom06g00780 pycom16g25890
rosa_chinensis	RchiOBHm_Chr7g0215501
rosa_laevigata	RLG00000002657
rosa_multiflora	Rmu_sc0005928.1_g000005
rosa_roxburghii	Rroxscaffold_3G00244190
rosa_rugosa	Rorug07G0154100 Rorug07G0154200
rosa_samantha	Rh7AG290300 Rh7BG282300 Rh7CG310300 Rh7DG294100
rosa_wichuraiana	Rw7G024790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1655
AasI	GACNNNNNNGTC	1 cut(s)	827
Acc36I	ACCTGC	2 cut(s)	368, 988
Acc65I	GGTACC	1 cut(s)	503
AccB1I	GGYRCC	1 cut(s)	503
AccI	GTMKAC	2 cut(s)	580, 2098
AciI	CCGC	1 cut(s)	2222
AclWI	GGATC	7 cut(s)	202, 805, 1004, 1484, 1497, 1589, 2005
AcoI	YGGCCR	1 cut(s)	3085
AcsI	RAATTY	6 cut(s)	511, 527, 550, 1067, 1498, 2559
AcuI	CTGAAG	4 cut(s)	117, 2594, 3051, 3601
AcvI	CACGTG	1 cut(s)	2976
AfiI	CCNNNNNNNGG	7 cut(s)	432, 773, 774, 1171, 2144, 2353, 3499
AflII	CTTAAG	2 cut(s)	325, 1826
AflIII	ACRYGT	3 cut(s)	1059, 2975, 3282
AjnI	CCWGG	7 cut(s)	86, 601, 1207, 2434, 2566, 2946, 3797
Alw21I	GWGCWC	5 cut(s)	547, 1548, 2555, 2723, 3225
Alw26I	GTCTC	2 cut(s)	1964, 3484
Alw44I	GTGCAC	1 cut(s)	2551
AlwI	GGATC	7 cut(s)	202, 805, 1004, 1484, 1497, 1589, 2005
AlwNI	CAGNNNCTG	1 cut(s)	3482
Ama87I	CYCGRG	1 cut(s)	3216
AoxI	GGCC	4 cut(s)	1184, 1205, 1728, 3085
ApaLI	GTGCAC	1 cut(s)	2551
ApeKI	GCWGC	9 cut(s)	254, 587, 1220, 1276, 2229, 2273, 2810, 3212, 3381
ApoI	RAATTY	6 cut(s)	511, 527, 550, 1067, 1498, 2559
ArsI	GACNNNNNNTTYG	2 cut(s)	205, 237
AseI	ATTAAT	1 cut(s)	1683
Asp700I	GAANNNNTTC	2 cut(s)	344, 3036
Asp718I	GGTACC	1 cut(s)	503
AspLEI	GCGC	1 cut(s)	1457
AspS9I	GGNCC	4 cut(s)	1205, 2177, 2651, 3776
AsuC2I	CCSGG	1 cut(s)	905
AsuHPI	GGTGA	7 cut(s)	476, 600, 701, 728, 1241, 1311, 1919
AsuNHI	GCTAGC	1 cut(s)	2537
AvaI	CYCGRG	1 cut(s)	3216
AvaII	GGWCC	3 cut(s)	2177, 2651, 3776
BaeGI	GKGCMC	1 cut(s)	2555
BalI	TGGCCA	1 cut(s)	3087
BamHI	GGATCC	1 cut(s)	1489
BanI	GGYRCC	1 cut(s)	503
BanII	GRGCYC	1 cut(s)	3225
BbrPI	CACGTG	1 cut(s)	2976
BbsI	GAAGAC	2 cut(s)	1917, 3607
Bbv12I	GWGCWC	5 cut(s)	547, 1548, 2555, 2723, 3225
BbvI	GCAGC	9 cut(s)	266, 599, 1232, 1288, 2241, 2260, 2797, 3199, 3393
BccI	CCATC	7 cut(s)	134, 181, 941, 2188, 2927, 3019, 3442
BceAI	ACGGC	2 cut(s)	818, 2135
BcgI	CGANNNNNNTGC	4 cut(s)	2987, 3021, 3775, 3809
BciT130I	CCWGG	7 cut(s)	88, 603, 1209, 2436, 2568, 2948, 3799
BciVI	GTATCC	1 cut(s)	1049
BclI	TGATCA	1 cut(s)	2905
BcnI	CCSGG	1 cut(s)	905
BcoDI	GTCTC	2 cut(s)	1964, 3484
BfmI	CTRYAG	5 cut(s)	1134, 1842, 2686, 2941, 3336
BfrI	CTTAAG	2 cut(s)	325, 1826
BfuAI	ACCTGC	2 cut(s)	368, 988
BfuI	GTATCC	1 cut(s)	1049
BglII	AGATCT	2 cut(s)	2992, 3433
BisI	GCNGC	9 cut(s)	255, 588, 1221, 1277, 2230, 2274, 2811, 3213, 3382
BlpI	GCTNAGC	2 cut(s)	1752, 2802
BlsI	GCNGC	9 cut(s)	256, 589, 1222, 1278, 2231, 2275, 2812, 3214, 3383
Bme1390I	CCNGG	8 cut(s)	88, 603, 905, 1209, 2436, 2568, 2948, 3799
Bme18I	GGWCC	3 cut(s)	2177, 2651, 3776
BmeT110I	CYCGRG	1 cut(s)	3216
BmgT120I	GGNCC	4 cut(s)	1205, 2177, 2651, 3776
BmiI	GGNNCC	7 cut(s)	169, 505, 1491, 2653, 2938, 3047, 3778
BmrFI	CCNGG	8 cut(s)	88, 603, 905, 1209, 2436, 2568, 2948, 3799
BmrI	ACTGGG	1 cut(s)	710
BmsI	GCATC	7 cut(s)	1291, 1652, 1837, 2124, 2780, 3043, 3617
BmtI	GCTAGC	1 cut(s)	2541
BmuI	ACTGGG	1 cut(s)	710
BpiI	GAAGAC	2 cut(s)	1917, 3607
BplI	GAGNNNNNCTC	2 cut(s)	3199, 3231
BpmI	CTGGAG	1 cut(s)	1986
Bpu1102I	GCTNAGC	2 cut(s)	1752, 2802
BpuEI	CTTGAG	1 cut(s)	3652
BpuMI	CCSGG	1 cut(s)	905
BsaAI	YACGTR	1 cut(s)	2976
BsaBI	GATNNNNATC	2 cut(s)	2604, 3570
BsaI	GGTCTC	1 cut(s)	1964
BsaJI	CCNNGG	8 cut(s)	903, 1208, 1638, 2947, 3041, 3088, 3797, 3798
BsaWI	WCCGGW	2 cut(s)	627, 1164
BsaXI	ACNNNNNCTCC	2 cut(s)	349, 379
Bsc4I	CCNNNNNNNGG	7 cut(s)	432, 773, 774, 1171, 2144, 2353, 3499
Bse118I	RCCGGY	1 cut(s)	3754
Bse1I	ACTGG	9 cut(s)	78, 238, 705, 758, 855, 1458, 2106, 2538, 3747
Bse3DI	GCAATG	4 cut(s)	2164, 2541, 2796, 3426
Bse8I	GATNNNNATC	2 cut(s)	2604, 3570
BseBI	CCWGG	7 cut(s)	88, 603, 1209, 2436, 2568, 2948, 3799
BseDI	CCNNGG	8 cut(s)	903, 1208, 1638, 2947, 3041, 3088, 3797, 3798
BseGI	GGATG	8 cut(s)	317, 496, 647, 728, 949, 2055, 2180, 2771
BseJI	GATNNNNATC	2 cut(s)	2604, 3570
BseLI	CCNNNNNNNGG	7 cut(s)	432, 773, 774, 1171, 2144, 2353, 3499
BseMI	GCAATG	4 cut(s)	2164, 2541, 2796, 3426
BseMII	CTCAG	5 cut(s)	74, 1179, 1743, 2343, 3003
BseNI	ACTGG	9 cut(s)	78, 238, 705, 758, 855, 1458, 2106, 2538, 3747
BseRI	GAGGAG	2 cut(s)	1578, 1905
BseSI	GKGCMC	1 cut(s)	2555
BseXI	GCAGC	9 cut(s)	266, 599, 1232, 1288, 2241, 2260, 2797, 3199, 3393
BseYI	CCCAGC	1 cut(s)	1598
BsgI	GTGCAG	1 cut(s)	2259
BshFI	GGCC	4 cut(s)	1186, 1207, 1730, 3087
BshNI	GGYRCC	1 cut(s)	503
BsiHKAI	GWGCWC	5 cut(s)	547, 1548, 2555, 2723, 3225
BsiHKCI	CYCGRG	1 cut(s)	3216
BsiSI	CCGG	4 cut(s)	628, 905, 1165, 3755
BsiWI	CGTACG	1 cut(s)	162
BslFI	GGGAC	1 cut(s)	3762
BslI	CCNNNNNNNGG	7 cut(s)	432, 773, 774, 1171, 2144, 2353, 3499
BsmAI	GTCTC	2 cut(s)	1964, 3484
BsmFI	GGGAC	1 cut(s)	3762
BsmI	GAATGC	1 cut(s)	91
BsnI	GGCC	4 cut(s)	1186, 1207, 1730, 3087
Bso31I	GGTCTC	1 cut(s)	1964
BsoBI	CYCGRG	1 cut(s)	3216
Bsp1286I	GDGCHC	5 cut(s)	547, 1548, 2555, 2723, 3225
Bsp1407I	TGTACA	2 cut(s)	248, 782
Bsp1720I	GCTNAGC	2 cut(s)	1752, 2802
Bsp19I	CCATGG	2 cut(s)	3041, 3088
BspACI	CCGC	1 cut(s)	2222
BspANI	GGCC	4 cut(s)	1186, 1207, 1730, 3087
BspCNI	CTCAG	5 cut(s)	73, 1180, 1744, 2344, 3002
BspLI	GGNNCC	7 cut(s)	169, 505, 1491, 2653, 2938, 3047, 3778
BspMAI	CTGCAG	1 cut(s)	1846
BspMI	ACCTGC	2 cut(s)	368, 988
BspOI	GCTAGC	1 cut(s)	2541
BspPI	GGATC	7 cut(s)	202, 805, 1004, 1484, 1497, 1589, 2005
BspT107I	GGYRCC	1 cut(s)	503
BspTI	CTTAAG	2 cut(s)	325, 1826
BspTNI	GGTCTC	1 cut(s)	1964
BsrDI	GCAATG	4 cut(s)	2164, 2541, 2796, 3426
BsrFI	RCCGGY	1 cut(s)	3754
BsrGI	TGTACA	2 cut(s)	248, 782
BsrI	ACTGG	9 cut(s)	78, 238, 705, 758, 855, 1458, 2106, 2538, 3747
BssAI	RCCGGY	1 cut(s)	3754
BssECI	CCNNGG	8 cut(s)	903, 1208, 1638, 2947, 3041, 3088, 3797, 3798
BssNAI	GTATAC	1 cut(s)	2099
BssT1I	CCWWGG	2 cut(s)	3041, 3088
Bst1107I	GTATAC	1 cut(s)	2099
Bst2UI	CCWGG	7 cut(s)	88, 603, 1209, 2436, 2568, 2948, 3799
Bst4CI	ACNGT	8 cut(s)	158, 167, 427, 736, 787, 924, 1114, 1476
Bst6I	CTCTTC	2 cut(s)	350, 3070
BstAFI	CTTAAG	2 cut(s)	325, 1826
BstAPI	GCANNNNNTGC	1 cut(s)	1768
BstAUI	TGTACA	2 cut(s)	248, 782
BstBAI	YACGTR	1 cut(s)	2976
BstC8I	GCNNGC	7 cut(s)	291, 322, 2247, 2445, 2449, 2539, 2808
BstDEI	CTNAG	8 cut(s)	60, 204, 1188, 1752, 2352, 2480, 2802, 2989
BstDSI	CCRYGG	2 cut(s)	3041, 3088
BstENI	CCTNNNNNAGG	1 cut(s)	2142
BstF5I	GGATG	8 cut(s)	317, 496, 647, 728, 949, 2055, 2180, 2771
BstHHI	GCGC	1 cut(s)	1457
BstMAI	GTCTC	2 cut(s)	1964, 3484
BstNI	CCWGG	7 cut(s)	88, 603, 1209, 2436, 2568, 2948, 3799
BstNSI	RCATGY	5 cut(s)	324, 1063, 3286, 3381, 3545
BstSCI	CCNGG	8 cut(s)	86, 601, 903, 1207, 2434, 2566, 2946, 3797
BstSFI	CTRYAG	5 cut(s)	1134, 1842, 2686, 2941, 3336
BstSLI	GKGCMC	1 cut(s)	2555
BstV1I	GCAGC	9 cut(s)	266, 599, 1232, 1288, 2241, 2260, 2797, 3199, 3393
BstV2I	GAAGAC	2 cut(s)	1917, 3607
BstX2I	RGATCY	6 cut(s)	207, 797, 1009, 1489, 2992, 3433
BstYI	RGATCY	6 cut(s)	207, 797, 1009, 1489, 2992, 3433
BstZ17I	GTATAC	1 cut(s)	2099
BsuI	GTATCC	1 cut(s)	1049
BsuRI	GGCC	4 cut(s)	1186, 1207, 1730, 3087
BtgI	CCRYGG	2 cut(s)	3041, 3088
BtsCI	GGATG	8 cut(s)	317, 496, 647, 728, 949, 2055, 2180, 2771
BtsI	GCAGTG	6 cut(s)	1036, 1602, 1719, 1839, 3112, 3510
BveI	ACCTGC	2 cut(s)	368, 988
Cac8I	GCNNGC	7 cut(s)	291, 322, 2247, 2445, 2449, 2539, 2808
CaiI	CAGNNNCTG	1 cut(s)	3482
CfoI	GCGC	1 cut(s)	1457
Cfr10I	RCCGGY	1 cut(s)	3754
Cfr13I	GGNCC	4 cut(s)	1205, 2177, 2651, 3776
CseI	GACGC	1 cut(s)	3135
CsiI	ACCWGGT	1 cut(s)	2434
CspCI	CAANNNNNGTGG	4 cut(s)	1495, 1530, 3007, 3042
DdeI	CTNAG	8 cut(s)	60, 204, 1188, 1752, 2352, 2480, 2802, 2989
DraI	TTTAAA	1 cut(s)	556
DrdI	GACNNNNNNGTC	1 cut(s)	827
DseDI	GACNNNNNNGTC	1 cut(s)	827
EaeI	YGGCCR	1 cut(s)	3085
Eam1104I	CTCTTC	2 cut(s)	350, 3070
EarI	CTCTTC	2 cut(s)	350, 3070
Ecl136II	GAGCTC	1 cut(s)	3223
Eco130I	CCWWGG	2 cut(s)	3041, 3088
Eco147I	AGGCCT	2 cut(s)	1186, 1730
Eco24I	GRGCYC	1 cut(s)	3225
Eco31I	GGTCTC	1 cut(s)	1964
Eco32I	GATATC	2 cut(s)	1413, 1426
Eco47I	GGWCC	3 cut(s)	2177, 2651, 3776
Eco53kI	GAGCTC	1 cut(s)	3223
Eco57I	CTGAAG	4 cut(s)	117, 2594, 3051, 3601
Eco72I	CACGTG	1 cut(s)	2976
Eco88I	CYCGRG	1 cut(s)	3216
EcoICRI	GAGCTC	1 cut(s)	3223
EcoNI	CCTNNNNNAGG	1 cut(s)	2142
EcoRI	GAATTC	3 cut(s)	511, 527, 2559
EcoRII	CCWGG	7 cut(s)	86, 601, 1207, 2434, 2566, 2946, 3797
EcoRV	GATATC	2 cut(s)	1413, 1426
EcoT14I	CCWWGG	2 cut(s)	3041, 3088
EcoT38I	GRGCYC	1 cut(s)	3225
ErhI	CCWWGG	2 cut(s)	3041, 3088
FalI	AAGNNNNNCTT	8 cut(s)	442, 474, 1575, 1607, 1809, 1841, 3021, 3053
FaqI	GGGAC	1 cut(s)	3762
FauNDI	CATATG	3 cut(s)	22, 613, 3534
FbaI	TGATCA	1 cut(s)	2905
FblI	GTMKAC	2 cut(s)	580, 2098
Fnu4HI	GCNGC	9 cut(s)	255, 588, 1221, 1277, 2230, 2274, 2811, 3213, 3382
FokI	GGATG	8 cut(s)	324, 503, 634, 715, 956, 2042, 2167, 2758
FriOI	GRGCYC	1 cut(s)	3225
Fsp4HI	GCNGC	9 cut(s)	255, 588, 1221, 1277, 2230, 2274, 2811, 3213, 3382
GlaI	GCGC	1 cut(s)	1456
GluI	GCNGC	9 cut(s)	255, 588, 1221, 1277, 2230, 2274, 2811, 3213, 3382
GsaI	CCCAGC	1 cut(s)	1602
GsuI	CTGGAG	1 cut(s)	1986
HaeIII	GGCC	4 cut(s)	1186, 1207, 1730, 3087
HapII	CCGG	4 cut(s)	628, 905, 1165, 3755
HgaI	GACGC	1 cut(s)	3135
HhaI	GCGC	1 cut(s)	1457
Hin6I	GCGC	1 cut(s)	1455
HinP1I	GCGC	1 cut(s)	1455
HincII	GTYRAC	2 cut(s)	1378, 3490
HindII	GTYRAC	2 cut(s)	1378, 3490
HindIII	AAGCTT	1 cut(s)	2853
HpaI	GTTAAC	1 cut(s)	3490
HpaII	CCGG	4 cut(s)	628, 905, 1165, 3755
HphI	GGTGA	7 cut(s)	476, 600, 701, 728, 1241, 1311, 1919
HpyAV	CCTTC	5 cut(s)	1720, 2138, 2312, 2452, 3792
HpyCH4III	ACNGT	8 cut(s)	158, 167, 427, 736, 787, 924, 1114, 1476
HpyCH4IV	ACGT	3 cut(s)	133, 1532, 2975
HpyF3I	CTNAG	8 cut(s)	60, 204, 1188, 1752, 2352, 2480, 2802, 2989
HpySE526I	ACGT	3 cut(s)	133, 1532, 2975
HspAI	GCGC	1 cut(s)	1455
KpnI	GGTACC	1 cut(s)	507
Ksp22I	TGATCA	1 cut(s)	2905
KspAI	GTTAAC	1 cut(s)	3490
LmnI	GCTCC	6 cut(s)	595, 1591, 2476, 2726, 3092, 3802
Lsp1109I	GCAGC	9 cut(s)	266, 599, 1232, 1288, 2241, 2260, 2797, 3199, 3393
LweI	GCATC	7 cut(s)	1291, 1652, 1837, 2124, 2780, 3043, 3617
MabI	ACCWGGT	1 cut(s)	2434
MaeII	ACGT	3 cut(s)	133, 1532, 2975
MfeI	CAATTG	2 cut(s)	1044, 2714
MflI	RGATCY	6 cut(s)	207, 797, 1009, 1489, 2992, 3433
MhlI	GDGCHC	5 cut(s)	547, 1548, 2555, 2723, 3225
MlsI	TGGCCA	1 cut(s)	3087
MluNI	TGGCCA	1 cut(s)	3087
MlyI	GAGTC	3 cut(s)	473, 638, 1904
MmeI	TCCRAC	5 cut(s)	248, 409, 1060, 2155, 2849
Mox20I	TGGCCA	1 cut(s)	3087
MroXI	GAANNNNTTC	2 cut(s)	344, 3036
MscI	TGGCCA	1 cut(s)	3087
MslI	CAYNNNNRTG	2 cut(s)	13, 3443
Msp20I	TGGCCA	1 cut(s)	3087
MspA1I	CMGCKG	1 cut(s)	752
MspCI	CTTAAG	2 cut(s)	325, 1826
MspI	CCGG	4 cut(s)	628, 905, 1165, 3755
MspR9I	CCNGG	8 cut(s)	88, 603, 905, 1209, 2436, 2568, 2948, 3799
MunI	CAATTG	2 cut(s)	1044, 2714
Mva1269I	GAATGC	1 cut(s)	91
MvaI	CCWGG	7 cut(s)	88, 603, 1209, 2436, 2568, 2948, 3799
NciI	CCSGG	1 cut(s)	905
NcoI	CCATGG	2 cut(s)	3041, 3088
NdeI	CATATG	3 cut(s)	22, 613, 3534
NheI	GCTAGC	1 cut(s)	2537
NlaIV	GGNNCC	7 cut(s)	169, 505, 1491, 2653, 2938, 3047, 3778
NmuCI	GTSAC	7 cut(s)	188, 391, 1247, 1317, 1330, 1907, 2984
NspI	RCATGY	5 cut(s)	324, 1063, 3286, 3381, 3545
PaeI	GCATGC	1 cut(s)	324
PaeR7I	CTCGAG	1 cut(s)	3216
PasI	CCCWGGG	1 cut(s)	3798
PceI	AGGCCT	2 cut(s)	1186, 1730
PciI	ACATGT	2 cut(s)	1059, 3282
PctI	GAATGC	1 cut(s)	91
PdmI	GAANNNNTTC	2 cut(s)	344, 3036
Pfl23II	CGTACG	1 cut(s)	162
PkrI	GCNGC	9 cut(s)	256, 589, 1222, 1278, 2231, 2275, 2812, 3214, 3383
PleI	GAGTC	3 cut(s)	473, 638, 1904
PmaCI	CACGTG	1 cut(s)	2976
PmlI	CACGTG	1 cut(s)	2976
PpsI	GAGTC	3 cut(s)	473, 638, 1904
Ppu21I	YACGTR	1 cut(s)	2976
PscI	ACATGT	2 cut(s)	1059, 3282
PshBI	ATTAAT	1 cut(s)	1683
PsiI	TTATAA	1 cut(s)	1655
Psp124BI	GAGCTC	1 cut(s)	3225
Psp6I	CCWGG	7 cut(s)	86, 601, 1207, 2434, 2566, 2946, 3797
PspCI	CACGTG	1 cut(s)	2976
PspFI	CCCAGC	1 cut(s)	1598
PspGI	CCWGG	7 cut(s)	86, 601, 1207, 2434, 2566, 2946, 3797
PspLI	CGTACG	1 cut(s)	162
PspN4I	GGNNCC	7 cut(s)	169, 505, 1491, 2653, 2938, 3047, 3778
PspPI	GGNCC	4 cut(s)	1205, 2177, 2651, 3776
PsrI	GAACNNNNNNTAC	2 cut(s)	146, 178
PstI	CTGCAG	1 cut(s)	1846
PstNI	CAGNNNCTG	1 cut(s)	3482
PsuI	RGATCY	6 cut(s)	207, 797, 1009, 1489, 2992, 3433
PvuII	CAGCTG	1 cut(s)	752
RseI	CAYNNNNRTG	2 cut(s)	13, 3443
SacI	GAGCTC	1 cut(s)	3225
SatI	GCNGC	9 cut(s)	255, 588, 1221, 1277, 2230, 2274, 2811, 3213, 3382
Sau96I	GGNCC	4 cut(s)	1205, 2177, 2651, 3776
SchI	GAGTC	3 cut(s)	473, 638, 1904
ScrFI	CCNGG	8 cut(s)	88, 603, 905, 1209, 2436, 2568, 2948, 3799
SduI	GDGCHC	5 cut(s)	547, 1548, 2555, 2723, 3225
SexAI	ACCWGGT	1 cut(s)	2434
SfaNI	GCATC	7 cut(s)	1291, 1652, 1837, 2124, 2780, 3043, 3617
SfcI	CTRYAG	5 cut(s)	1134, 1842, 2686, 2941, 3336
Sfr274I	CTCGAG	1 cut(s)	3216
SinI	GGWCC	3 cut(s)	2177, 2651, 3776
SlaI	CTCGAG	1 cut(s)	3216
SmiMI	CAYNNNNRTG	2 cut(s)	13, 3443
SmlI	CTYRAG	4 cut(s)	325, 1826, 3216, 3667
SmoI	CTYRAG	4 cut(s)	325, 1826, 3216, 3667
SphI	GCATGC	1 cut(s)	324
SseBI	AGGCCT	2 cut(s)	1186, 1730
SsiI	CCGC	1 cut(s)	2222
SspI	AATATT	2 cut(s)	104, 1860
SstI	GAGCTC	1 cut(s)	3225
StuI	AGGCCT	2 cut(s)	1186, 1730
StyD4I	CCNGG	8 cut(s)	86, 601, 903, 1207, 2434, 2566, 2946, 3797
StyI	CCWWGG	2 cut(s)	3041, 3088
TaaI	ACNGT	8 cut(s)	158, 167, 427, 736, 787, 924, 1114, 1476
TaiI	ACGT	3 cut(s)	136, 1535, 2978
TaqI	TCGA	7 cut(s)	981, 3014, 3051, 3217, 3255, 3303, 3785
TatI	WGTACW	3 cut(s)	248, 782, 3655
TseFI	GTSAC	7 cut(s)	188, 391, 1247, 1317, 1330, 1907, 2984
TseI	GCWGC	9 cut(s)	254, 587, 1220, 1276, 2229, 2273, 2810, 3212, 3381
Tsp45I	GTSAC	7 cut(s)	188, 391, 1247, 1317, 1330, 1907, 2984
TspGWI	ACGGA	6 cut(s)	150, 289, 1349, 1395, 1801, 1978
Vha464I	CTTAAG	2 cut(s)	325, 1826
VneI	GTGCAC	1 cut(s)	2551
VpaK11BI	GGWCC	3 cut(s)	2177, 2651, 3776
VspI	ATTAAT	1 cut(s)	1683
XagI	CCTNNNNNAGG	1 cut(s)	2142
XapI	RAATTY	6 cut(s)	511, 527, 550, 1067, 1498, 2559
XbaI	TCTAGA	2 cut(s)	515, 3230
XceI	RCATGY	5 cut(s)	324, 1063, 3286, 3381, 3545
XhoI	CTCGAG	1 cut(s)	3216
XmiI	GTMKAC	2 cut(s)	580, 2098
XmnI	GAANNNNTTC	2 cut(s)	344, 3036

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.