AT1G44835

Prolyl-tRNA synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009587
Physical Location & Seq
Forward (+)
16939692 .. 16942558
2867 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G44835.2

Sequence Viewer

Length: 924 bp
ATGGGTTTCACCAAAGATCAGTTGCTTGATCGACTTCAGGAACTTGAGATTGATTATTCCAAGTATGAACATCCGCCTGTACTAACCGTCGAAGAACAGGCCAAGTATGTTAGCAGTAGTAAGGGTGCACTGAGTAAGAATCTCTTCTTGAAGGACAAGAAACATCGGTATTATATTGTTTCAGCGATGGTGGATACGAAAGTTGACATGAAAGTTTTATCTCAGAGACTTGGTCTTGGAAAAGGAGGGATTAGAATGGCTCCAGAAGAAGCACTTGGTGAGCTTTTGCAGGTATCCCTCGGATGTGTTACCCCGTTTGCAGTTGTAAATGAATCAGCAAGAGACGTGTCTCTCTTGTTAGACCAAAAATTCAAGAACCAAACGCGTTGCATCTTCCACCCATTGTCTAACGATGTCTCAGTCTCTCTGAACACATTGGGTCTGGACAAGTTTCTGAAGTCGATTGGGAGAGATCCCGTATACGTTGACCTTGAGGCTAACCCGGTAGTTGGTAAAGACCAGGCTCCAGATCTAGCTGTTTGTGTTCCGTCTAATTCAGTTATTGTTCCCGAGATTCCTAATCAAACATCTTCTACACAAATTCCTCTTCCAAAAAGTGTGTCAGCAGAGGTCAAGCCTGTGGCTTCAGCCAAAACGAGCAAGCCAGCTTGTAAGGTGAAGAGCGTCGCTGAAAATTCAGCTCCATCAGCTTACAAGAACCCGGAGAAATTTGTGCAGGAGATTTTGGACAAAACTTCGGCTCTATTATTGTCCGAGGCTAAGGGAGAGTGCGTGGAGGCTTTGGCAGAAACACTAAGAAAACGACTTACCTCAGAGTTGACCCACCTCTCTATTATGTACAAGAACTCAGCGTACGCAGAAGGTTTTTATGCTGGTACACGCCACCAGCCGAAGCGATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2 tRNA_edit

Protein Analysis

307

Amino Acids

33.71

Weight (kDa)

8.62

Isoelectric Point (pI)

32.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_edit PF04073 24 - 151 1.1e-30 Aminoacyl-tRNA editing domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 280
AccI GTMKAC 1 cut(s) 480
AccII CGCG 1 cut(s) 385
AciI CCGC 1 cut(s) 74
AclWI GGATC 1 cut(s) 467
AcsI RAATTY 4 cut(s) 368, 600, 694, 728
AcuI CTGAAG 3 cut(s) 20, 476, 630
AdeI CACNNNGTG 1 cut(s) 278
AfaI GTAC 4 cut(s) 81, 860, 875, 898
AfiI CCNNNNNNNGG 1 cut(s) 509
AflIII ACRYGT 2 cut(s) 345, 383
AgsI TTSAA 2 cut(s) 151, 373
AjiI CACGTC 1 cut(s) 346
AjnI CCWGG 1 cut(s) 519
AjuI GAANNNNNNNTTGG 2 cut(s) 258, 290
AluBI AGCT 5 cut(s) 283, 536, 668, 701, 710
AluI AGCT 5 cut(s) 283, 536, 668, 701, 710
Alw21I GWGCWC 1 cut(s) 130
Alw26I GTCTC 5 cut(s) 220, 336, 354, 421, 427
Alw44I GTGCAC 1 cut(s) 126
AlwI GGATC 1 cut(s) 467
Ama87I CYCGRG 1 cut(s) 569
AoxI GGCC 1 cut(s) 99
ApaLI GTGCAC 1 cut(s) 126
ApoI RAATTY 4 cut(s) 368, 600, 694, 728
ArsI GACNNNNNNTTYG 2 cut(s) 605, 637
Asp700I GAANNNNTTC 1 cut(s) 143
AsuC2I CCSGG 2 cut(s) 503, 722
AsuHPI GGTGA 2 cut(s) 290, 688
AvaI CYCGRG 1 cut(s) 569
BaeGI GKGCMC 1 cut(s) 130
BaeI ACNNNNGTAYC 2 cut(s) 186, 219
Bbv12I GWGCWC 1 cut(s) 130
BccI CCATC 2 cut(s) 181, 712
BciT130I CCWGG 1 cut(s) 521
BciVI GTATCC 2 cut(s) 187, 304
BcnI CCSGG 2 cut(s) 503, 722
BcoDI GTCTC 5 cut(s) 220, 336, 354, 421, 427
BfaI CTAG 1 cut(s) 533
BfuAI ACCTGC 1 cut(s) 280
BfuI GTATCC 2 cut(s) 187, 304
BglII AGATCT 1 cut(s) 529
Bme1390I CCNGG 3 cut(s) 503, 521, 722
BmeT110I CYCGRG 1 cut(s) 569
BmgBI CACGTC 1 cut(s) 346
BmiI GGNNCC 2 cut(s) 261, 525
BmrFI CCNGG 3 cut(s) 503, 521, 722
BmsI GCATC 1 cut(s) 399
BpmI CTGGAG 2 cut(s) 246, 510
Bpu10I CCTNAGC 1 cut(s) 780
BpuEI CTTGAG 2 cut(s) 65, 512
BpuMI CCSGG 2 cut(s) 503, 722
BsaJI CCNNGG 2 cut(s) 298, 774
BsaXI ACNNNNNCTCC 2 cut(s) 777, 807
Bsc4I CCNNNNNNNGG 1 cut(s) 509
BseBI CCWGG 1 cut(s) 521
BseDI CCNNGG 2 cut(s) 298, 774
BseGI GGATG 2 cut(s) 70, 308
BseLI CCNNNNNNNGG 1 cut(s) 509
BseMII CTCAG 5 cut(s) 122, 236, 432, 846, 882
BseSI GKGCMC 1 cut(s) 130
BsgI GTGCAG 1 cut(s) 755
Bsh1236I CGCG 1 cut(s) 385
BshFI GGCC 1 cut(s) 101
BsiHKAI GWGCWC 1 cut(s) 130
BsiHKCI CYCGRG 1 cut(s) 569
BsiSI CCGG 2 cut(s) 503, 722
BsiWI CGTACG 1 cut(s) 873
BslI CCNNNNNNNGG 1 cut(s) 509
BsmAI GTCTC 5 cut(s) 220, 336, 354, 421, 427
BsmBI CGTCTC 1 cut(s) 336
BsnI GGCC 1 cut(s) 101
BsoBI CYCGRG 1 cut(s) 569
Bsp1286I GDGCHC 1 cut(s) 130
Bsp1407I TGTACA 1 cut(s) 858
Bsp143I GATC 4 cut(s) 16, 28, 472, 529
BspACI CCGC 1 cut(s) 74
BspANI GGCC 1 cut(s) 101
BspCNI CTCAG 5 cut(s) 123, 235, 431, 845, 881
BspFNI CGCG 1 cut(s) 385
BspLI GGNNCC 2 cut(s) 261, 525
BspMI ACCTGC 1 cut(s) 280
BspPI GGATC 1 cut(s) 467
BspQI GCTCTTC 1 cut(s) 674
BsrGI TGTACA 1 cut(s) 858
BssECI CCNNGG 2 cut(s) 298, 774
BssMI GATC 4 cut(s) 16, 28, 472, 529
BssNAI GTATAC 1 cut(s) 481
Bst1107I GTATAC 1 cut(s) 481
Bst2UI CCWGG 1 cut(s) 521
Bst4CI ACNGT 1 cut(s) 88
Bst6I CTCTTC 3 cut(s) 149, 612, 674
BstAUI TGTACA 1 cut(s) 858
BstC8I GCNNGC 2 cut(s) 662, 666
BstDEI CTNAG 7 cut(s) 131, 222, 418, 780, 815, 832, 868
BstF5I GGATG 2 cut(s) 70, 308
BstFNI CGCG 1 cut(s) 385
BstKTI GATC 4 cut(s) 19, 31, 475, 532
BstMAI GTCTC 5 cut(s) 220, 336, 354, 421, 427
BstMBI GATC 4 cut(s) 16, 28, 472, 529
BstMWI GCNNNNNNNGC 1 cut(s) 707
BstNI CCWGG 1 cut(s) 521
BstSCI CCNGG 3 cut(s) 501, 519, 720
BstSLI GKGCMC 1 cut(s) 130
BstUI CGCG 1 cut(s) 385
BstX2I RGATCY 2 cut(s) 472, 529
BstYI RGATCY 2 cut(s) 472, 529
BstZ17I GTATAC 1 cut(s) 481
BsuI GTATCC 2 cut(s) 187, 304
BsuRI GGCC 1 cut(s) 101
BtgZI GCGATG 1 cut(s) 200
BtrI CACGTC 1 cut(s) 346
BtsCI GGATG 2 cut(s) 70, 308
BtsIMutI CAGTG 1 cut(s) 128
BveI ACCTGC 1 cut(s) 280
Cac8I GCNNGC 2 cut(s) 662, 666
CseI GACGC 1 cut(s) 673
Csp6I GTAC 4 cut(s) 80, 859, 874, 897
CviAII CATG 1 cut(s) 208
CviQI GTAC 4 cut(s) 80, 859, 874, 897
DdeI CTNAG 7 cut(s) 131, 222, 418, 780, 815, 832, 868
DpnI GATC 4 cut(s) 18, 30, 474, 531
DpnII GATC 4 cut(s) 16, 28, 472, 529
DraIII CACNNNGTG 1 cut(s) 278
Eam1104I CTCTTC 3 cut(s) 149, 612, 674
EarI CTCTTC 3 cut(s) 149, 612, 674
EciI GGCGGA 1 cut(s) 63
Eco57I CTGAAG 3 cut(s) 20, 476, 630
Eco88I CYCGRG 1 cut(s) 569
EcoRII CCWGG 1 cut(s) 519
Esp3I CGTCTC 1 cut(s) 336
FaeI CATG 1 cut(s) 211
FaiI YATR 7 cut(s) 66, 108, 174, 209, 481, 857, 891
FalI AAGNNNNNCTT 4 cut(s) 128, 160, 258, 290
FatI CATG 1 cut(s) 207
FblI GTMKAC 1 cut(s) 480
FokI GGATG 2 cut(s) 57, 315
FspBI CTAG 1 cut(s) 533
GsuI CTGGAG 2 cut(s) 246, 510
HaeIII GGCC 1 cut(s) 101
HapII CCGG 2 cut(s) 503, 722
HgaI GACGC 1 cut(s) 673
Hin1II CATG 1 cut(s) 211
HincII GTYRAC 3 cut(s) 205, 487, 840
HindII GTYRAC 3 cut(s) 205, 487, 840
HinfI GANTC 3 cut(s) 139, 332, 574
HpaII CCGG 2 cut(s) 503, 722
HphI GGTGA 2 cut(s) 290, 688
Hpy166II GTNNAC 6 cut(s) 128, 205, 481, 487, 840, 899
Hpy188I TCNGA 6 cut(s) 225, 302, 429, 456, 775, 835
Hpy188III TCNNGA 7 cut(s) 38, 148, 263, 373, 443, 527, 569
Hpy8I GTNNAC 6 cut(s) 128, 205, 481, 487, 840, 899
Hpy99I CGWCG 2 cut(s) 92, 689
HpyAV CCTTC 2 cut(s) 145, 875
HpyCH4III ACNGT 1 cut(s) 88
HpyCH4IV ACGT 2 cut(s) 345, 483
HpyCH4V TGCA 5 cut(s) 128, 289, 320, 390, 736
HpyF10VI GCNNNNNNNGC 1 cut(s) 707
HpyF3I CTNAG 7 cut(s) 131, 222, 418, 780, 815, 832, 868
HpySE526I ACGT 2 cut(s) 345, 483
Hsp92II CATG 1 cut(s) 211
Kzo9I GATC 4 cut(s) 16, 28, 472, 529
LguI GCTCTTC 1 cut(s) 674
LmnI GCTCC 3 cut(s) 265, 529, 706
LweI GCATC 1 cut(s) 399
MaeI CTAG 1 cut(s) 533
MaeII ACGT 2 cut(s) 345, 483
MaeIII GTNAC 1 cut(s) 307
MalI GATC 4 cut(s) 18, 30, 474, 531
MboI GATC 4 cut(s) 16, 28, 472, 529
MboII GAAGA 7 cut(s) 104, 136, 278, 385, 582, 599, 691
MflI RGATCY 2 cut(s) 472, 529
MhlI GDGCHC 1 cut(s) 130
MluCI AATT 5 cut(s) 368, 553, 600, 694, 728
MluI ACGCGT 1 cut(s) 383
MnlI CCTC 9 cut(s) 239, 308, 487, 615, 622, 769, 790, 841, 857
MroXI GAANNNNTTC 1 cut(s) 143
MspI CCGG 2 cut(s) 503, 722
MspR9I CCNGG 3 cut(s) 503, 521, 722
MvaI CCWGG 1 cut(s) 521
MvnI CGCG 1 cut(s) 385
MwoI GCNNNNNNNGC 1 cut(s) 707
NciI CCSGG 2 cut(s) 503, 722
NdeII GATC 4 cut(s) 16, 28, 472, 529
NlaIII CATG 1 cut(s) 211
NlaIV GGNNCC 2 cut(s) 261, 525
PciSI GCTCTTC 1 cut(s) 674
PdmI GAANNNNTTC 1 cut(s) 143
PfeI GAWTC 3 cut(s) 139, 332, 574
Pfl23II CGTACG 1 cut(s) 873
PflFI GACNNNGTC 1 cut(s) 231
Psp6I CCWGG 1 cut(s) 519
PspGI CCWGG 1 cut(s) 519
PspLI CGTACG 1 cut(s) 873
PspN4I GGNNCC 2 cut(s) 261, 525
PsrI GAACNNNNNNTAC 2 cut(s) 857, 889
PsuI RGATCY 2 cut(s) 472, 529
PsyI GACNNNGTC 1 cut(s) 231
RsaI GTAC 4 cut(s) 81, 860, 875, 898
RsaNI GTAC 4 cut(s) 80, 859, 874, 897
SapI GCTCTTC 1 cut(s) 674
Sau3AI GATC 4 cut(s) 16, 28, 472, 529
ScrFI CCNGG 3 cut(s) 503, 521, 722
SduI GDGCHC 1 cut(s) 130
SfaNI GCATC 1 cut(s) 399
SmlI CTYRAG 2 cut(s) 44, 491
SmoI CTYRAG 2 cut(s) 44, 491
Sse9I AATT 5 cut(s) 368, 553, 600, 694, 728
SsiI CCGC 1 cut(s) 74
SspMI CTAG 1 cut(s) 533
StyD4I CCNGG 3 cut(s) 501, 519, 720
TaaI ACNGT 1 cut(s) 88
TaiI ACGT 2 cut(s) 348, 486
TaqI TCGA 3 cut(s) 31, 90, 461
TasI AATT 5 cut(s) 368, 553, 600, 694, 728
TatI WGTACW 2 cut(s) 79, 858
TfiI GAWTC 3 cut(s) 139, 332, 574
TscAI CASTG 1 cut(s) 135
TspDTI ATGAA 3 cut(s) 81, 224, 345
TspGWI ACGGA 1 cut(s) 537
TspRI CASTG 1 cut(s) 135
Tth111I GACNNNGTC 1 cut(s) 231
VneI GTGCAC 1 cut(s) 126
XapI RAATTY 4 cut(s) 368, 600, 694, 728
XmiI GTMKAC 1 cut(s) 480
XmnI GAANNNNTTC 1 cut(s) 143
XspI CTAG 1 cut(s) 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.