Rw6G031810

prolyl-tRNA synthetase associated domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0009587
Physical Location & Seq
Reverse (-)
55896815 .. 55900485
3671 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G031810.1

Sequence Viewer

Length: 945 bp
ATGGGTCTTTCCAAGGAACACTTGCTTGCCCGCTTAAAGGAGCTTCAGATTGATTTTTCCCAGTATGATCATCCTGTTGTTTTGACAGTTGAGGCTCAGGCAAAATATGTTGGACATCTGGGAGGTGGACTCAGTAAAAATTTGTTCTTGAAGGATAAGAAGAGTAGGTTCTATATTGTTTCCGCTTTGGCAGATACTAAAGTAGATCTGAAAGTTCTATCTGTGAGGCTTGGTTTGGGGAAAGGTGGCTTGAGAATGGCTCCTGAAGAAGCACTGGGAGAAATACTCCAGGTCCCATTGGGTTGTGTTACTCCTTTATCACTGGTGAACGAATCTGCATGCAATGTCTCTCTGCTGTTGGATCAAAAATTTAAAAGTCAGGAGTTTTGCTTCTTCCACCCATTATCGAATGACATGTCAATCTCACTAACCACCAATGGTCTCGACAAGTTTCTTAAGTCAATTAAGAGAGACCCCTCATATGTTGACTTGGAGGCTAACCCATCGGTTGGGAAGGACCAACCTCCTGATCTTGCATCTCTTGTTCCATCTAGTTCCGTAGTATTGCCAGATCCCCCGGAAACAGCAGCTTCCTCGAAAGATCGCACAGAAAATACAGTTTCTGTGGGTAACAAGTCCACAAAAAGTACAGTGAGCACTTCTAAGCCATCCATTAATGCTAAAAAAACCCAGGATAAACCAGCCAACATAACAGATGTTGGAAAAATGGTTGATGACTTGCTGGATTCAACATCTTCGCTAGTGCTGAAAGAGATTACAGAGGAGAATATTAAGGAACATGGAGAAGAGCTTGGAGAATTGTTATCAAAGAAAATTAAAAATCAATTAGCTGCAGAATTGCACAACACGATTATGATATTCAAAAACACTGCATACACAGAAGGATTCCACGCTGGGATGCACTGTCATCCAAAGCGTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2 tRNA_edit

Protein Analysis

314

Amino Acids

34.21

Weight (kDa)

6.76

Isoelectric Point (pI)

36.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_edit PF04073 24 - 151 1.8e-26 Aminoacyl-tRNA editing domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 509
AciI CCGC 2 cut(s) 31, 183
AclWI GGATC 2 cut(s) 369, 566
AcsI RAATTY 2 cut(s) 139, 368
AcuI CTGAAG 2 cut(s) 29, 285
AfaI GTAC 1 cut(s) 649
AfiI CCNNNNNNNGG 2 cut(s) 37, 509
AflII CTTAAG 1 cut(s) 455
AflIII ACRYGT 1 cut(s) 414
AgsI TTSAA 3 cut(s) 151, 750, 883
AjnI CCWGG 2 cut(s) 288, 690
AluBI AGCT 4 cut(s) 43, 590, 811, 851
AluI AGCT 4 cut(s) 43, 590, 811, 851
Alw21I GWGCWC 1 cut(s) 659
Alw26I GTCTC 3 cut(s) 352, 446, 465
AlwI GGATC 2 cut(s) 369, 566
AlwNI CAGNNNCTG 1 cut(s) 623
ApeKI GCWGC 2 cut(s) 587, 851
ApoI RAATTY 2 cut(s) 139, 368
ArsI GACNNNNNNTTYG 2 cut(s) 401, 433
AseI ATTAAT 1 cut(s) 675
AspS9I GGNCC 2 cut(s) 292, 517
AsuC2I CCSGG 1 cut(s) 578
AsuHPI GGTGA 1 cut(s) 337
AvaII GGWCC 2 cut(s) 292, 517
BaeI ACNNNNGTAYC 2 cut(s) 186, 219
Bbv12I GWGCWC 1 cut(s) 659
BbvI GCAGC 2 cut(s) 599, 838
BccI CCATC 3 cut(s) 511, 556, 676
BcgI CGANNNNNNTGC 2 cut(s) 576, 610
BciT130I CCWGG 2 cut(s) 290, 692
BclI TGATCA 1 cut(s) 67
BcnI CCSGG 1 cut(s) 578
BcoDI GTCTC 3 cut(s) 352, 446, 465
BfaI CTAG 2 cut(s) 552, 761
BfmI CTRYAG 1 cut(s) 852
BfrI CTTAAG 1 cut(s) 455
BglII AGATCT 1 cut(s) 205
BisI GCNGC 2 cut(s) 588, 852
BlsI GCNGC 2 cut(s) 589, 853
Bme1390I CCNGG 3 cut(s) 290, 578, 692
Bme18I GGWCC 2 cut(s) 292, 517
BmgT120I GGNCC 2 cut(s) 292, 517
BmiI GGNNCC 2 cut(s) 261, 294
BmrFI CCNGG 3 cut(s) 290, 578, 692
BmrI ACTGGG 2 cut(s) 55, 284
BmsI GCATC 2 cut(s) 545, 909
BmuI ACTGGG 2 cut(s) 55, 284
BplI GAGNNNNNCTC 6 cut(s) 114, 146, 244, 276, 270, 302
BpmI CTGGAG 1 cut(s) 272
Bpu10I CCTNAGC 1 cut(s) 96
BpuEI CTTGAG 1 cut(s) 271
BpuMI CCSGG 1 cut(s) 578
BsaI GGTCTC 2 cut(s) 446, 465
BsaJI CCNNGG 3 cut(s) 12, 576, 690
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 509
Bse1I ACTGG 3 cut(s) 61, 279, 327
Bse3DI GCAATG 1 cut(s) 349
BseBI CCWGG 2 cut(s) 290, 692
BseDI CCNNGG 3 cut(s) 12, 576, 690
BseGI GGATG 4 cut(s) 70, 668, 924, 928
BseLI CCNNNNNNNGG 2 cut(s) 37, 509
BseMI GCAATG 1 cut(s) 349
BseMII CTCAG 2 cut(s) 110, 145
BseNI ACTGG 3 cut(s) 61, 279, 327
BseRI GAGGAG 1 cut(s) 797
BseXI GCAGC 2 cut(s) 599, 838
BseYI CCCAGC 1 cut(s) 914
BsiHKAI GWGCWC 1 cut(s) 659
BsiSI CCGG 1 cut(s) 578
BslFI GGGAC 1 cut(s) 278
BslI CCNNNNNNNGG 2 cut(s) 37, 509
BsmAI GTCTC 3 cut(s) 352, 446, 465
BsmFI GGGAC 1 cut(s) 278
Bso31I GGTCTC 2 cut(s) 446, 465
Bsp1286I GDGCHC 1 cut(s) 659
Bsp143I GATC 6 cut(s) 67, 205, 361, 529, 571, 601
BspACI CCGC 2 cut(s) 31, 183
BspCNI CTCAG 2 cut(s) 109, 144
BspLI GGNNCC 2 cut(s) 261, 294
BspMAI CTGCAG 1 cut(s) 856
BspPI GGATC 2 cut(s) 369, 566
BspQI GCTCTTC 1 cut(s) 801
BspTI CTTAAG 1 cut(s) 455
BspTNI GGTCTC 2 cut(s) 446, 465
BsrDI GCAATG 1 cut(s) 349
BsrI ACTGG 3 cut(s) 61, 279, 327
BssECI CCNNGG 3 cut(s) 12, 576, 690
BssMI GATC 6 cut(s) 67, 205, 361, 529, 571, 601
BssT1I CCWWGG 1 cut(s) 12
Bst2UI CCWGG 2 cut(s) 290, 692
Bst4CI ACNGT 4 cut(s) 88, 619, 652, 926
Bst6I CTCTTC 2 cut(s) 155, 801
BstAFI CTTAAG 1 cut(s) 455
BstC8I GCNNGC 3 cut(s) 27, 31, 340
BstDEI CTNAG 4 cut(s) 96, 131, 663, 942
BstF5I GGATG 4 cut(s) 70, 668, 924, 928
BstKTI GATC 6 cut(s) 70, 208, 364, 532, 574, 604
BstMAI GTCTC 3 cut(s) 352, 446, 465
BstMBI GATC 6 cut(s) 67, 205, 361, 529, 571, 601
BstNI CCWGG 2 cut(s) 290, 692
BstNSI RCATGY 2 cut(s) 342, 418
BstSCI CCNGG 3 cut(s) 288, 576, 690
BstSFI CTRYAG 1 cut(s) 852
BstV1I GCAGC 2 cut(s) 599, 838
BstX2I RGATCY 2 cut(s) 205, 571
BstYI RGATCY 2 cut(s) 205, 571
BtsCI GGATG 4 cut(s) 70, 668, 924, 928
BtsI GCAGTG 1 cut(s) 888
BtsIMutI CAGTG 5 cut(s) 272, 320, 657, 888, 922
Cac8I GCNNGC 3 cut(s) 27, 31, 340
CaiI CAGNNNCTG 1 cut(s) 623
Cfr13I GGNCC 2 cut(s) 292, 517
Csp6I GTAC 1 cut(s) 648
CviAII CATG 3 cut(s) 339, 415, 800
CviQI GTAC 1 cut(s) 648
DdeI CTNAG 4 cut(s) 96, 131, 663, 942
DpnI GATC 6 cut(s) 69, 207, 363, 531, 573, 603
DpnII GATC 6 cut(s) 67, 205, 361, 529, 571, 601
DraI TTTAAA 1 cut(s) 373
Eam1104I CTCTTC 2 cut(s) 155, 801
EarI CTCTTC 2 cut(s) 155, 801
Eco130I CCWWGG 1 cut(s) 12
Eco31I GGTCTC 2 cut(s) 446, 465
Eco47I GGWCC 2 cut(s) 292, 517
Eco57I CTGAAG 2 cut(s) 29, 285
EcoO109I RGGNCCY 1 cut(s) 292
EcoRII CCWGG 2 cut(s) 288, 690
EcoT14I CCWWGG 1 cut(s) 12
ErhI CCWWGG 1 cut(s) 12
FaeI CATG 3 cut(s) 342, 418, 803
FalI AAGNNNNNCTT 1 cut(s) 37
FaqI GGGAC 1 cut(s) 278
FatI CATG 3 cut(s) 338, 414, 799
FauI CCCGC 1 cut(s) 38
FauNDI CATATG 1 cut(s) 481
FbaI TGATCA 1 cut(s) 67
Fnu4HI GCNGC 2 cut(s) 588, 852
FokI GGATG 4 cut(s) 57, 655, 915, 931
Fsp4HI GCNGC 2 cut(s) 588, 852
FspBI CTAG 2 cut(s) 552, 761
GluI GCNGC 2 cut(s) 588, 852
GsaI CCCAGC 1 cut(s) 918
GsuI CTGGAG 1 cut(s) 272
HapII CCGG 1 cut(s) 578
Hin1II CATG 3 cut(s) 342, 418, 803
HincII GTYRAC 1 cut(s) 487
HindII GTYRAC 1 cut(s) 487
HinfI GANTC 4 cut(s) 129, 332, 746, 906
HpaII CCGG 1 cut(s) 578
HphI GGTGA 1 cut(s) 337
Hpy166II GTNNAC 4 cut(s) 128, 328, 487, 639
Hpy188I TCNGA 2 cut(s) 48, 210
Hpy188III TCNNGA 5 cut(s) 148, 263, 380, 443, 527
Hpy8I GTNNAC 4 cut(s) 128, 328, 487, 639
HpyAV CCTTC 3 cut(s) 145, 508, 896
HpyCH4III ACNGT 4 cut(s) 88, 619, 652, 926
HpyCH4V TGCA 7 cut(s) 338, 342, 536, 854, 862, 893, 922
HpyF3I CTNAG 4 cut(s) 96, 131, 663, 942
Hsp92II CATG 3 cut(s) 342, 418, 803
Ksp22I TGATCA 1 cut(s) 67
Kzo9I GATC 6 cut(s) 67, 205, 361, 529, 571, 601
LguI GCTCTTC 1 cut(s) 801
LmnI GCTCC 2 cut(s) 40, 265
Lsp1109I GCAGC 2 cut(s) 599, 838
LweI GCATC 2 cut(s) 545, 909
MaeI CTAG 2 cut(s) 552, 761
MaeIII GTNAC 2 cut(s) 307, 629
MalI GATC 6 cut(s) 69, 207, 363, 531, 573, 603
MboI GATC 6 cut(s) 67, 205, 361, 529, 571, 601
MboII GAAGA 5 cut(s) 172, 278, 385, 747, 818
MflI RGATCY 2 cut(s) 205, 571
MhlI GDGCHC 1 cut(s) 659
MluCI AATT 7 cut(s) 139, 368, 462, 818, 834, 845, 857
MlyI GAGTC 1 cut(s) 123
MmeI TCCRAC 3 cut(s) 91, 339, 700
MnlI CCTC 8 cut(s) 85, 116, 219, 487, 487, 534, 604, 775
MseI TTAA 7 cut(s) 35, 372, 456, 465, 675, 792, 837
MslI CAYNNNNRTG 1 cut(s) 872
MspCI CTTAAG 1 cut(s) 455
MspI CCGG 1 cut(s) 578
MspR9I CCNGG 3 cut(s) 290, 578, 692
MvaI CCWGG 2 cut(s) 290, 692
NciI CCSGG 1 cut(s) 578
NdeI CATATG 1 cut(s) 481
NdeII GATC 6 cut(s) 67, 205, 361, 529, 571, 601
NlaIII CATG 3 cut(s) 342, 418, 803
NlaIV GGNNCC 2 cut(s) 261, 294
NspI RCATGY 2 cut(s) 342, 418
PaeI GCATGC 1 cut(s) 342
PciI ACATGT 1 cut(s) 414
PciSI GCTCTTC 1 cut(s) 801
PfeI GAWTC 3 cut(s) 332, 746, 906
PflMI CCANNNNNTGG 1 cut(s) 509
PkrI GCNGC 2 cut(s) 589, 853
PleI GAGTC 1 cut(s) 123
PpsI GAGTC 1 cut(s) 123
PpuMI RGGWCCY 1 cut(s) 292
PscI ACATGT 1 cut(s) 414
PshBI ATTAAT 1 cut(s) 675
Psp5II RGGWCCY 1 cut(s) 292
Psp6I CCWGG 2 cut(s) 288, 690
PspFI CCCAGC 1 cut(s) 914
PspGI CCWGG 2 cut(s) 288, 690
PspN4I GGNNCC 2 cut(s) 261, 294
PspPI GGNCC 2 cut(s) 292, 517
PspPPI RGGWCCY 1 cut(s) 292
PstI CTGCAG 1 cut(s) 856
PstNI CAGNNNCTG 1 cut(s) 623
PsuI RGATCY 2 cut(s) 205, 571
RsaI GTAC 1 cut(s) 649
RsaNI GTAC 1 cut(s) 648
RseI CAYNNNNRTG 1 cut(s) 872
SapI GCTCTTC 1 cut(s) 801
SaqAI TTAA 7 cut(s) 35, 372, 456, 465, 675, 792, 837
SatI GCNGC 2 cut(s) 588, 852
Sau3AI GATC 6 cut(s) 67, 205, 361, 529, 571, 601
Sau96I GGNCC 2 cut(s) 292, 517
SchI GAGTC 1 cut(s) 123
ScrFI CCNGG 3 cut(s) 290, 578, 692
SduI GDGCHC 1 cut(s) 659
SetI ASST 9 cut(s) 45, 127, 170, 247, 294, 526, 592, 813, 853
SfaNI GCATC 2 cut(s) 545, 909
SfcI CTRYAG 1 cut(s) 852
SinI GGWCC 2 cut(s) 292, 517
SmiMI CAYNNNNRTG 1 cut(s) 872
SmlI CTYRAG 2 cut(s) 250, 455
SmoI CTYRAG 2 cut(s) 250, 455
SphI GCATGC 1 cut(s) 342
Sse9I AATT 7 cut(s) 139, 368, 462, 818, 834, 845, 857
SsiI CCGC 2 cut(s) 31, 183
SspI AATATT 1 cut(s) 790
SspMI CTAG 2 cut(s) 552, 761
StyD4I CCNGG 3 cut(s) 288, 576, 690
StyI CCWWGG 1 cut(s) 12
TaaI ACNGT 4 cut(s) 88, 619, 652, 926
TaqI TCGA 3 cut(s) 407, 444, 596
TasI AATT 7 cut(s) 139, 368, 462, 818, 834, 845, 857
TatI WGTACW 1 cut(s) 647
TfiI GAWTC 3 cut(s) 332, 746, 906
Tru1I TTAA 7 cut(s) 35, 372, 456, 465, 675, 792, 837
Tru9I TTAA 7 cut(s) 35, 372, 456, 465, 675, 792, 837
TscAI CASTG 5 cut(s) 279, 327, 657, 895, 929
TseI GCWGC 2 cut(s) 587, 851
TspGWI ACGGA 1 cut(s) 547
TspRI CASTG 5 cut(s) 279, 327, 657, 895, 929
Van91I CCANNNNNTGG 1 cut(s) 509
Vha464I CTTAAG 1 cut(s) 455
VpaK11BI GGWCC 2 cut(s) 292, 517
VspI ATTAAT 1 cut(s) 675
XapI RAATTY 2 cut(s) 139, 368
XceI RCATGY 2 cut(s) 342, 418
XspI CTAG 2 cut(s) 552, 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.