MD15G1352500.v1.1

prolyl-tRNA synthetase associated domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009587
Physical Location & Seq
Forward (+)
42134526 .. 42139582
5057 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1352500.v1.1.491

Sequence Viewer

Length: 966 bp
ATGGGTCTCTCCAAGGAACAGTTGCTTGCCCACTTAAAGGAGCTACAAGTCGATTTTTCCCACCATGAACATCCTGTTGTTCTGACAGTTGAAGCTCAGGCCAAATATGTTGGACATCTGGGAGGTGGACTAAGTAAAAATTTATTCCTGAAGGACAAGAAAAGTAGGTTCTACATTGTTTCCGCTTTGGCAGATACTAAAGTAGATCTGAAAGTTCTATCTGTGAGGCTTGGTTTGGGAAAAGGTGGCTTGAGAATGGCTCCTGAAGAAGCACTTGGAGAAATACTTCAGGTGCCTCTGGGATGTGTTACTCCATTTTCTGTGGTGAATGAATCTGCACGCCATGTCTCTCTGTTGTTGGATCAAAAATTTAGAAGTCAGGAGCGGTGCTTCTTCCACCCTTTGTCGAATGACATGTCAATCTCACTTAGTACCCATGATCTTGACAAGTTTCTAAAATCAATTGGGAGAGACCCCTCATATGTTGACTTGGAGGCTAACCCATCGGTTGGAAAGGATCAACCACCTGATCTTGCATGCTTTGTTCCGTCTGGTTCCACTGTGTTGCCAGACCCTCCACAACCAGCAGCTTCCTCTAAAGGTCATGCAGAAATTCAAGTTTCTGTCTCTAACAAATCAACAGCAAGTACAGTCTCTTCTAAGCCATCCAGTAGTGCAAAAAGTGCCAAGGACAAACAGGTCAATGGTGTGAATGTGTCGAAGACCGTAGCAGATGTTGGAAGTTTGGTTGAAGATTTACTCGATAGAACATCTTCTCTATTGCTTTCAGAGATCACTGAGGAGACTATTAAGAAACATGAAGGACAATTGGGAACTAAGGTATCAGATAGTATCAGAAGCCGACTTCGAGAAGATTTCGAGAGAACCCTTATGATATTTAAATCCACCGCATATACCGAAGGGTTTTCTGCTGGCAAGCACCATCATCCACACCGTACGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2 tRNA_edit

Protein Analysis

322

Amino Acids

34.95

Weight (kDa)

7.79

Isoelectric Point (pI)

34.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_edit PF04073 24 - 151 8.6e-28 Aminoacyl-tRNA editing domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 698
AccB1I GGYRCC 1 cut(s) 292
AccB7I CCANNNNNTGG 1 cut(s) 509
AccBSI CCGCTC 1 cut(s) 385
AciI CCGC 3 cut(s) 183, 385, 909
AclWI GGATC 2 cut(s) 369, 525
AcsI RAATTY 3 cut(s) 139, 368, 612
AcuI CTGAAG 3 cut(s) 170, 272, 285
AfaI GTAC 3 cut(s) 433, 649, 958
AfiI CCNNNNNNNGG 2 cut(s) 37, 509
AflIII ACRYGT 1 cut(s) 414
AgsI TTSAA 3 cut(s) 92, 617, 752
AjuI GAANNNNNNNTTGG 2 cut(s) 258, 290
AluBI AGCT 3 cut(s) 43, 95, 590
AluI AGCT 3 cut(s) 43, 95, 590
Alw26I GTCTC 6 cut(s) 11, 352, 465, 631, 658, 797
AlwI GGATC 2 cut(s) 369, 525
AoxI GGCC 1 cut(s) 99
ApeKI GCWGC 1 cut(s) 587
ApoI RAATTY 3 cut(s) 139, 368, 612
ArsI GACNNNNNNTTYG 2 cut(s) 401, 433
Asp700I GAANNNNTTC 2 cut(s) 285, 772
AsuHPI GGTGA 1 cut(s) 337
BaeI ACNNNNGTAYC 4 cut(s) 186, 219, 825, 858
BanI GGYRCC 1 cut(s) 292
BarI GAAGNNNNNNTAC 2 cut(s) 640, 672
BbsI GAAGAC 1 cut(s) 728
BbvI GCAGC 1 cut(s) 599
BccI CCATC 3 cut(s) 511, 673, 951
BcoDI GTCTC 6 cut(s) 11, 352, 465, 631, 658, 797
BglII AGATCT 1 cut(s) 205
BisI GCNGC 1 cut(s) 588
BlsI GCNGC 1 cut(s) 589
BmiI GGNNCC 3 cut(s) 261, 294, 556
BpiI GAAGAC 1 cut(s) 728
BplI GAGNNNNNCTC 2 cut(s) 244, 276
Bpu10I CCTNAGC 1 cut(s) 96
BpuEI CTTGAG 1 cut(s) 271
BsaAI YACGTR 1 cut(s) 960
BsaI GGTCTC 2 cut(s) 11, 465
BsaJI CCNNGG 2 cut(s) 12, 687
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 509
Bse1I ACTGG 1 cut(s) 669
BseDI CCNNGG 2 cut(s) 12, 687
BseGI GGATG 4 cut(s) 70, 308, 665, 946
BseLI CCNNNNNNNGG 2 cut(s) 37, 509
BseMII CTCAG 2 cut(s) 110, 789
BseNI ACTGG 1 cut(s) 669
BseRI GAGGAG 1 cut(s) 815
BseXI GCAGC 1 cut(s) 599
BsgI GTGCAG 1 cut(s) 321
BshFI GGCC 1 cut(s) 101
BshNI GGYRCC 1 cut(s) 292
BsiWI CGTACG 1 cut(s) 956
BslI CCNNNNNNNGG 2 cut(s) 37, 509
BsmAI GTCTC 6 cut(s) 11, 352, 465, 631, 658, 797
BsnI GGCC 1 cut(s) 101
Bso31I GGTCTC 2 cut(s) 11, 465
Bsp143I GATC 6 cut(s) 205, 361, 439, 517, 529, 792
BspACI CCGC 3 cut(s) 183, 385, 909
BspANI GGCC 1 cut(s) 101
BspCNI CTCAG 2 cut(s) 109, 790
BspLI GGNNCC 3 cut(s) 261, 294, 556
BspPI GGATC 2 cut(s) 369, 525
BspT107I GGYRCC 1 cut(s) 292
BspTNI GGTCTC 2 cut(s) 11, 465
BsrBI CCGCTC 1 cut(s) 385
BsrI ACTGG 1 cut(s) 669
BssECI CCNNGG 2 cut(s) 12, 687
BssMI GATC 6 cut(s) 205, 361, 439, 517, 529, 792
BssT1I CCWWGG 2 cut(s) 12, 687
Bst4CI ACNGT 6 cut(s) 21, 88, 562, 652, 727, 956
Bst6I CTCTTC 1 cut(s) 661
BstAPI GCANNNNNTGC 1 cut(s) 683
BstBAI YACGTR 1 cut(s) 960
BstC8I GCNNGC 5 cut(s) 27, 340, 538, 934, 938
BstDEI CTNAG 6 cut(s) 96, 131, 428, 660, 798, 837
BstF5I GGATG 4 cut(s) 70, 308, 665, 946
BstKTI GATC 6 cut(s) 208, 364, 442, 520, 532, 795
BstMAI GTCTC 6 cut(s) 11, 352, 465, 631, 658, 797
BstMBI GATC 6 cut(s) 205, 361, 439, 517, 529, 792
BstMWI GCNNNNNNNGC 1 cut(s) 683
BstNSI RCATGY 2 cut(s) 418, 540
BstSNI TACGTA 1 cut(s) 960
BstV1I GCAGC 1 cut(s) 599
BstV2I GAAGAC 1 cut(s) 728
BstX2I RGATCY 1 cut(s) 205
BstYI RGATCY 1 cut(s) 205
BsuRI GGCC 1 cut(s) 101
BtsCI GGATG 4 cut(s) 70, 308, 665, 946
BtsIMutI CAGTG 2 cut(s) 558, 795
Cac8I GCNNGC 5 cut(s) 27, 340, 538, 934, 938
Csp6I GTAC 3 cut(s) 432, 648, 957
CviAII CATG 7 cut(s) 65, 344, 415, 437, 537, 605, 818
CviQI GTAC 3 cut(s) 432, 648, 957
DdeI CTNAG 6 cut(s) 96, 131, 428, 660, 798, 837
DpnI GATC 6 cut(s) 207, 363, 441, 519, 531, 794
DpnII GATC 6 cut(s) 205, 361, 439, 517, 529, 792
DraI TTTAAA 1 cut(s) 901
DrdI GACNNNNNNGTC 1 cut(s) 698
DseDI GACNNNNNNGTC 1 cut(s) 698
Eam1104I CTCTTC 1 cut(s) 661
EarI CTCTTC 1 cut(s) 661
Eco105I TACGTA 1 cut(s) 960
Eco130I CCWWGG 2 cut(s) 12, 687
Eco31I GGTCTC 2 cut(s) 11, 465
Eco57I CTGAAG 3 cut(s) 170, 272, 285
EcoT14I CCWWGG 2 cut(s) 12, 687
ErhI CCWWGG 2 cut(s) 12, 687
FaeI CATG 7 cut(s) 68, 347, 418, 440, 540, 608, 821
FalI AAGNNNNNCTT 2 cut(s) 258, 290
FatI CATG 7 cut(s) 64, 343, 414, 436, 536, 604, 817
FauNDI CATATG 1 cut(s) 481
Fnu4HI GCNGC 1 cut(s) 588
FokI GGATG 4 cut(s) 57, 315, 652, 933
Fsp4HI GCNGC 1 cut(s) 588
GluI GCNGC 1 cut(s) 588
HaeIII GGCC 1 cut(s) 101
Hin1II CATG 7 cut(s) 68, 347, 418, 440, 540, 608, 821
HincII GTYRAC 1 cut(s) 487
HindII GTYRAC 1 cut(s) 487
HinfI GANTC 1 cut(s) 332
HphI GGTGA 1 cut(s) 337
Hpy166II GTNNAC 2 cut(s) 128, 487
Hpy188I TCNGA 5 cut(s) 84, 210, 790, 847, 857
Hpy188III TCNNGA 6 cut(s) 148, 263, 380, 443, 869, 880
Hpy8I GTNNAC 2 cut(s) 128, 487
HpyAV CCTTC 3 cut(s) 145, 815, 914
HpyCH4III ACNGT 6 cut(s) 21, 88, 562, 652, 727, 956
HpyCH4IV ACGT 1 cut(s) 959
HpyCH4V TGCA 4 cut(s) 338, 536, 608, 677
HpyF10VI GCNNNNNNNGC 1 cut(s) 683
HpyF3I CTNAG 6 cut(s) 96, 131, 428, 660, 798, 837
HpySE526I ACGT 1 cut(s) 959
Hsp92II CATG 7 cut(s) 68, 347, 418, 440, 540, 608, 821
Kzo9I GATC 6 cut(s) 205, 361, 439, 517, 529, 792
LmnI GCTCC 3 cut(s) 40, 265, 382
Lsp1109I GCAGC 1 cut(s) 599
MaeII ACGT 1 cut(s) 959
MaeIII GTNAC 1 cut(s) 307
MalI GATC 6 cut(s) 207, 363, 441, 519, 531, 794
MbiI CCGCTC 1 cut(s) 385
MboI GATC 6 cut(s) 205, 361, 439, 517, 529, 792
MboII GAAGA 7 cut(s) 278, 385, 648, 733, 764, 765, 884
MfeI CAATTG 2 cut(s) 462, 827
MflI RGATCY 1 cut(s) 205
MluCI AATT 5 cut(s) 139, 368, 462, 612, 827
MmeI TCCRAC 4 cut(s) 91, 339, 490, 718
MnlI CCTC 8 cut(s) 116, 219, 306, 487, 487, 585, 604, 793
MroXI GAANNNNTTC 2 cut(s) 285, 772
MseI TTAA 3 cut(s) 35, 810, 900
MunI CAATTG 2 cut(s) 462, 827
MwoI GCNNNNNNNGC 1 cut(s) 683
NdeI CATATG 1 cut(s) 481
NdeII GATC 6 cut(s) 205, 361, 439, 517, 529, 792
NlaIII CATG 7 cut(s) 68, 347, 418, 440, 540, 608, 821
NlaIV GGNNCC 3 cut(s) 261, 294, 556
NspI RCATGY 2 cut(s) 418, 540
PaeI GCATGC 1 cut(s) 540
PciI ACATGT 1 cut(s) 414
PdmI GAANNNNTTC 2 cut(s) 285, 772
PfeI GAWTC 1 cut(s) 332
Pfl23II CGTACG 1 cut(s) 956
PflMI CCANNNNNTGG 1 cut(s) 509
PkrI GCNGC 1 cut(s) 589
Ppu21I YACGTR 1 cut(s) 960
PscI ACATGT 1 cut(s) 414
PspLI CGTACG 1 cut(s) 956
PspN4I GGNNCC 3 cut(s) 261, 294, 556
PsuI RGATCY 1 cut(s) 205
RsaI GTAC 3 cut(s) 433, 649, 958
RsaNI GTAC 3 cut(s) 432, 648, 957
SaqAI TTAA 3 cut(s) 35, 810, 900
SatI GCNGC 1 cut(s) 588
Sau3AI GATC 6 cut(s) 205, 361, 439, 517, 529, 792
SmiI ATTTAAAT 1 cut(s) 901
SmlI CTYRAG 1 cut(s) 250
SmoI CTYRAG 1 cut(s) 250
SnaBI TACGTA 1 cut(s) 960
SphI GCATGC 1 cut(s) 540
Sse9I AATT 5 cut(s) 139, 368, 462, 612, 827
SsiI CCGC 3 cut(s) 183, 385, 909
StyI CCWWGG 2 cut(s) 12, 687
SwaI ATTTAAAT 1 cut(s) 901
TaaI ACNGT 6 cut(s) 21, 88, 562, 652, 727, 956
TaiI ACGT 1 cut(s) 962
TaqI TCGA 6 cut(s) 51, 407, 719, 762, 868, 879
TasI AATT 5 cut(s) 139, 368, 462, 612, 827
TatI WGTACW 1 cut(s) 647
TfiI GAWTC 1 cut(s) 332
Tru1I TTAA 3 cut(s) 35, 810, 900
Tru9I TTAA 3 cut(s) 35, 810, 900
TscAI CASTG 2 cut(s) 565, 802
TseI GCWGC 1 cut(s) 587
TspDTI ATGAA 3 cut(s) 81, 345, 834
TspGWI ACGGA 1 cut(s) 537
TspRI CASTG 2 cut(s) 565, 802
Van91I CCANNNNNTGG 1 cut(s) 509
XapI RAATTY 3 cut(s) 139, 368, 612
XceI RCATGY 2 cut(s) 418, 540
XmnI GAANNNNTTC 2 cut(s) 285, 772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.